1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
|
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<!-- saved from url=(0062)http://ndbserver.rutgers.edu/NDB/NDBATLAS/P/pr0004/pr0004.html -->
<HTML><HEAD><TITLE>NDB Atlas Entry for Structure PR0004</TITLE>
<META http-equiv=Content-Type content="text/html; charset=windows-1252">
<META content="MSHTML 5.50.4134.600" name=GENERATOR></HEAD>
<BODY bgColor=#ffffff>
<H1>NDB ID: PR0004 </H1>
<P>
<H2>Nucleic acid features </H2>tRNA, modified, mismatch
<H2>Compound name </H2>ELONGATION FACTOR TU/CYSTEINYL TRNA COMPLEX
<H2>Sequence in asymmetric unit </H2>
<UL>
<LI>Chain A: G G C G C G U 4SU A A C A A A G C G G H2U H2U A U G U A G C G G A
PSU U G C A MIA A PSU C C G U C U A G U C C G G T PSU C G A C U C C G G A A C
G C G C C U C C A
<LI>Chain B: ALA LYS GLY GLU PHE ILE ARG THR LYS PRO HIS VAL ASN VAL GLY THR
ILE GLY HIS VAL ASP HIS GLY LYS THR THR LEU THR ALA ALA LEU THR TYR VAL ALA
ALA ALA GLU ASN PRO ASN VAL GLU VAL LYS ASP TYR GLY ASP ILE ASP LYS ALA PRO
GLU GLU ARG ALA ARG GLY ILE THR ILE ASN THR ALA HIS VAL GLU TYR GLU THR ALA
LYS ARG HIS TYR SER HIS VAL ASP CYS PRO GLY HIS ALA ASP TYR ILE LYS ASN MET
ILE THR GLY ALA ALA GLN MET ASP GLY ALA ILE LEU VAL VAL SER ALA ALA ASP GLY
PRO MET PRO GLN THR ARG GLU HIS ILE LEU LEU ALA ARG GLN VAL GLY VAL PRO TYR
ILE VAL VAL PHE MET ASN LYS VAL ASP MET VAL ASP ASP PRO GLU LEU LEU ASP LEU
VAL GLU MET GLU VAL ARG ASP LEU LEU ASN GLN TYR GLU PHE PRO GLY ASP GLU VAL
PRO VAL ILE ARG GLY SER ALA LEU LEU ALA LEU GLU GLU MET HIS LYS ASN PRO LYS
THR LYS ARG GLY GLU ASN GLU TRP VAL ASP LYS ILE TRP GLU LEU LEU ASP ALA ILE
ASP GLU TYR ILE PRO THR PRO VAL ARG ASP VAL ASP LYS PRO PHE LEU MET PRO VAL
GLU ASP VAL PHE THR ILE THR GLY ARG GLY THR VAL ALA THR GLY ARG ILE GLU ARG
GLY LYS VAL LYS VAL GLY ASP GLU VAL GLU ILE VAL GLY LEU ALA PRO GLU THR ARG
LYS THR VAL VAL THR GLY VAL GLU MET HIS ARG LYS THR LEU GLN GLU GLY ILE ALA
GLY ASP ASN VAL GLY LEU LEU LEU ARG GLY VAL SER ARG GLU GLU VAL GLU ARG GLY
GLN VAL LEU ALA LYS PRO GLY SER ILE THR PRO HIS THR LYS PHE GLU ALA SER VAL
TYR ILE LEU LYS LYS GLU GLU GLY GLY ARG HIS THR GLY PHE PHE THR GLY TYR ARG
PRO GLN PHE TYR PHE ARG THR THR ASP VAL THR GLY VAL VAL ARG LEU PRO GLN GLY
VAL GLU MET VAL MET PRO GLY ASP ASN VAL THR PHE THR VAL GLU LEU ILE LYS PRO
VAL ALA LEU GLU GLU GLY LEU ARG PHE ALA ILE ARG GLU GLY GLY ARG THR VAL GLY
ALA GLY VAL VAL THR LYS ILE LEU GLU </LI></UL>
<H2>Citation </H2>P. Nissen, S. Thirup, M. Kjeldgaard, J. Nyborg<BR>The Crystal
Structure of Cys-tRNA-EF-Tu-GDPNP Reveals General and Specific Features of the
Ternary Complex and in tRNA<BR><I>Structure,</I> <B>7</B>, pp. 143-156, 1999.
<P>
<H2>Space group </H2>F 2 2 2
<H2>Cell constants </H2><PRE> A = 126.750 B = 132.980 C = 154.880 (ngstroms)
alpha = 90.00 beta = 90.00 gamma = 90.00 (degrees)
</PRE>
<H2>Crystallization conditions </H2>
<UL>
<LI>Method: vapor diffusion, hanging drop
<LI>Drop: (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB>, tris, MES,
MgCl<SUB>2</SUB>, DTT </LI></UL>
<H2>Refinement </H2>The structure was refined using the X-PLOR 3.851 program.
The R value is 20.6 for 18043 reflections in the resolution range 10.0 to 2.6
with Fobs > 0.0 sigma(Fobs).
<P>
<H2>Coordinates </H2>The <A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/coords/pdb-coord/pr0004.pdb">coordinates
for the asymmetric unit</A> of this structure are stored in the NDB archive.
<P>
<P>
<H2>Views of PR0004 </H2><A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/P/pr0004/pr0004_1_gif.html">One
view of PR0004 </A>
<P><PRE>
</PRE>
<HR>
<P align=center><I>1995-1998 The Nucleic Acid Database Project<BR>Rutgers, The
State University of New Jersey </I></P></BODY></HTML>
|
