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<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<!-- saved from url=(0062)http://ndbserver.rutgers.edu/NDB/NDBATLAS/P/pr0007/pr0007.html -->
<HTML><HEAD><TITLE>NDB Atlas Entry for Structure PR0007</TITLE>
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<META content="MSHTML 5.50.4134.600" name=GENERATOR></HEAD>
<BODY bgColor=#ffffff>
<H1>NDB ID: PR0007 </H1>
<P>
<H2>Features </H2>RNA single strand
<H2>Compound name </H2>POLYDENYLATE BINDING PROTEIN 1/RNA COMPLEX
<H2>Sequence in asymmetric unit </H2>
<UL>
<LI>Chain A: A A A A A A A A A A A
<LI>Chain B: A A A A A A A A A A A
<LI>Chain C: A A A A A A A A A A A
<LI>Chain D: A A A A A A A A A A A
<LI>Chain E: A A A A A A A A A A A
<LI>Chain F: A A A A A A A A A A A
<LI>Chain G: A A A A A A A A A A A
<LI>Chain H: A A A A A A A A A A A
<LI>Chain I: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY
ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY
PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR
ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN
PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER
LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP
ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL
VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU
ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL
PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA
LYS GLU PHE
<LI>Chain J: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY
ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY
PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR
ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN
PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER
LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP
ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL
VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU
ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL
PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA
LYS GLU PHE
<LI>Chain K: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY
ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY
PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR
ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN
PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER
LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP
ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL
VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU
ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL
PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA
LYS GLU PHE
<LI>Chain L: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY
ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY
PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR
ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN
PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER
LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP
ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL
VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU
ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL
PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA
LYS GLU PHE
<LI>Chain M: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY
ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY
PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR
ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN
PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER
LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP
ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL
VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU
ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL
PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA
LYS GLU PHE
<LI>Chain N: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY
ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY
PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR
ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN
PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER
LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP
ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL
VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU
ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL
PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA
LYS GLU PHE
<LI>Chain O: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY
ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY
PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR
ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN
PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER
LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP
ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL
VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU
ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL
PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA
LYS GLU PHE
<LI>Chain P: MET ASN PRO SER ALA PRO SER TYR PRO MET ALA SER LEU TYR VAL GLY
ASP LEU HIS PRO ASP VAL THR GLU ALA MET LEU TYR GLU LYS PHE SER PRO ALA GLY
PRO ILE LEU SER ILE ARG VAL CYS ARG ASP MET ILE THR ARG ARG SER LEU GLY TYR
ALA TYR VAL ASN PHE GLN GLN PRO ALA ASP ALA GLU ARG ALA LEU ASP THR MET ASN
PHE ASP VAL ILE LYS GLY LYS PRO VAL ARG ILE MET TRP SER GLN ARG ASP PRO SER
LEU ARG LYS SER GLY VAL GLY ASN ILE PHE ILE LYS ASN LEU ASP LYS SER ILE ASP
ASN LYS ALA LEU TYR ASP THR PHE SER ALA PHE GLY ASN ILE LEU SER CYS LYS VAL
VAL CYS ASP GLU ASN GLY SER LYS GLY TYR GLY PHE VAL HIS PHE GLU THR GLN GLU
ALA ALA GLU ARG ALA ILE GLU LYS MET ASN GLY MET LEU LEU ASN ASP ARG LYS VAL
PHE VAL GLY ARG PHE LYS SER ARG LYS GLU ARG GLU ALA GLU LEU GLY ALA ARG ALA
LYS GLU PHE </LI></UL>
<H2>Citation </H2>R.C. Deo, J.B. Bonanno, N. Sonenberg, S.K.
Burley<BR>Recognition of polyadenylate RNA by the poly(A)-binding
protein<BR><I>Cell (Cambridge,Mass. ),</I> <B>17</B>, pp. 835-845, 1999.
<P>
<H2>Space group </H2>P 2<SUB>1</SUB>
<H2>Cell constants </H2><PRE> A = 79.557 B = 176.797 C = 82.489 (ngstroms)
alpha = 90.00 beta = 117.67 gamma = 90.00 (degrees)
</PRE>
<H2>Crystallization conditions </H2>
<UL>
<LI>Method: vapor diffusion, hanging drop
<LI>Drop: tcep, tris-hcl, KCl, (NH4)SO4, glycerol
<LI>Reservoir: (NH4)SO4, glycerol </LI></UL>
<H2>Refinement </H2>The structure was refined using the CNS program. The R value
is 23.0 for 57149 reflections in the resolution range 20.0 to 2.6 with Fobs
> 2.0 sigma(Fobs).
<P>
<H2>Coordinates </H2>The <A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/coords/pdb-coord/pr0007.pdb">coordinates
for the asymmetric unit</A> of this structure are stored in the NDB archive.
<P>
<P>
<H2>Views of PR0007 </H2><A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/P/pr0007/pr0007_1_gif.html">A
view of PR0007 </A>
<P><PRE>
</PRE>
<HR>
<P align=center><I>1995-1998 The Nucleic Acid Database Project<BR>Rutgers, The
State University of New Jersey </I></P></BODY></HTML>
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