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<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<!-- saved from url=(0062)http://ndbserver.rutgers.edu/NDB/NDBATLAS/U/urx035/urx035.html -->
<HTML><HEAD><TITLE>NDB Atlas Entry for Structure URX035</TITLE>
<META http-equiv=Content-Type content="text/html; charset=windows-1252">
<META content="MSHTML 5.50.4134.600" name=GENERATOR></HEAD>
<BODY bgColor=#ffffff>
<H2>NDB ID: URX035 </H2>
<P>
<H2>Features </H2>
<P>RNA, RNA hammerhead ribozyme, catalytic RNA, loop
<P>
<H2>Compound name </H2>RNA hammerhead ribozyme
<P>
<H2>Sequence in asymmetric unit </H2>
<UL>
<LI>Chain A: G U G G U C U G A U G A G G C C
<LI>Chain B: G G C C G A A A C U C G U A A G A G U C A C C A C
<LI>Chain C: G U G G U C U G A U G A G G C C
<LI>Chain D: G G C C G A A A C U C G U A A G A G U C A C C A C </LI></UL>
<H2>Citation </H2>W.G. Scott, J.T. Finch, A. Klug<BR>The Crystal Structure of an
All-RNA Hammerhead Ribozyme: A Proposed Mechanism for RNA Catalytic
Cleavage<BR><I>Cell,</I> <B>81</B>, pp. 991-1002, 1995.
<P>
<H2>Space group </H2>P3<SUB>1</SUB>21
<P>
<H2>Cell constants </H2><PRE> A = 64.980 B = 64.980 C = 138.140 (ngstroms)
alpha = 90.00 beta = 90.00 gamma = 120.00 (degrees)
</PRE>
<P>
<P>
<H2>Crystallization conditions </H2>
<UL>
<LI>Method: vapor diffusion, sitting drop
<LI>Solution: nucleic acid, water, PEG 6000, glycerol, spermine, magnesium
acetate, ammonium acetate, ammonium cacodylate </LI></UL>
<H2>Refinement </H2>The structure was refined using the X-PLOR 3.1 program. The
R value is 25.1 for 6199 reflections in the resolution range 15.0 to 3.1 with
Fobs > 2.0 sigma(Fobs).
<P>
<H2>Coordinates </H2>The <A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/coords/pdb-coord/urx035.pdb">coordinates
for the asymmetric unit</A> of this structure are stored in the NDB archive.
<P>
<P>
<H2>Views of URX035 </H2>
<P><A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/U/urx035/urx035_1_gif.html">Four
views of URX035 (biological unit 1) </A>
<P><A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/U/urx035/urx035_2_gif.html">Four
views of URX035 (biological unit 2) </A>
<P><A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/U/urx035/urx035_3_gif.html">The
asymmetric unit of URX035 </A>
<P><A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/U/urx035/urx035_4_gif.html">A
crystal packing picture of URX035 </A>
<P><A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/U/urx035/urx035_5_gif.html">Another
crystal packing picture </A>
<P><PRE>
</PRE>
<HR>
<P align=center><I>1995,1996 The Nucleic Acid Database Project<BR>Rutgers, The
State University of New Jersey </I></P></BODY></HTML>
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