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<HTML>
<HEAD>
<TITLE>BLAST Search Results </TITLE>
</HEAD>
<BODY BGCOLOR="#FFFFFF" LINK="#0000FF" VLINK="#660099" ALINK="#660099">
<A HREF="http://www.ncbi.nlm.nih.gov/BLAST/blast_form.map"> <IMG SRC="http://www.ncbi.nlm.nih.gov/BLAST/blast_results.gif" BORDER=0 ISMAP></A>
<BR><BR><PRE>
<b>BLASTX 2.0.10 [Aug-26-1999]</b>
<b><a href="http://www.ncbi.nlm.nih.gov/htbin-
post/Entrez/query?uid=9254694&form=6&db=m&Dopt=r">Reference</a>:</b>
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
<p>
<b>Query=</b> gi|1347369|gb|G25137.1|G25137 human STS EST48004.
(556 letters)
<b>Database:</b> Non-redundant SwissProt sequences
82,258 sequences; 29,652,561 total letters
<p> <p>If you have any problems or questions with the results of this search <br>please refer to the <b><a href=http://www.ncbi.nlm.nih.gov/BLAST/blast_FAQs.html>BLAST FAQs</a></b><br><p>
<FORM NAME="BLASTFORM">
</PRE>
<CENTER>
<H3><a href="/BLAST/newoptions.html#graphical-overview"> Distribution of 5 Blast Hits on the Query Sequence</a></H3>
<input name=defline size=80 value="Mouse-over to show defline and scores. Click to show alignments">
</CENTER>
<map name=img_map>
<area shape=rect coords=69,101,183,106 href="#1731448" ONMOUSEOVER='document.BLASTFORM.defline.value="P54103 ZUOTIN RELATED FACTOR-1..S=86.6 E=3e-17"' ONMOUSEOUT='document.BLASTFORM.defline.value="Mouse-over to show defline and scores. Click to show alignments"' >
<area shape=rect coords=96,108,395,113 href="#465911" ONMOUSEOVER='document.BLASTFORM.defline.value="P34454 HYPOTHETICAL 31.6 KD PROTEIN F54F2.9 IN CHROMOSOME III..S=41.8 E=0.001"' ONMOUSEOUT='document.BLASTFORM.defline.value="Mouse-over to show defline and scores. Click to show alignments"' >
<area shape=rect coords=69,115,395,120 href="#2494160" ONMOUSEOVER='document.BLASTFORM.defline.value="Q61712 DNAJ PROTEIN HOMOLOG MTJ1..S=36.7 E=0.033"' ONMOUSEOUT='document.BLASTFORM.defline.value="Mouse-over to show defline and scores. Click to show alignments"' >
<area shape=rect coords=110,122,353,127 href="#1730688" ONMOUSEOVER='document.BLASTFORM.defline.value="P53745 HYPOTHETICAL 68.1 KD PROTEIN IN BIO3-FRE4 INTERGENIC REGIO..S=29.0 E=7.4"' ONMOUSEOUT='document.BLASTFORM.defline.value="Mouse-over to show defline and scores. Click to show alignments"' >
</map>
<CENTER>
<IMG WIDTH=529 HEIGHT=129 USEMAP=#img_map BORDER=1 SRC="nph-getgif.cgi?miles&207891705327933.gif" ISMAP></CENTER>
<HR>
<PRE>
<PRE>
Score E
Sequences producing significant alignments: (bits) Value
<a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=01731448&dopt=GenPept">sp|P54103|ZRF1_MOUSE</a> ZUOTIN RELATED FACTOR-1 <a href = #1731448> 87</a> 3e-17
<a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=00465911&dopt=GenPept">sp|P34454|YMA9_CAEEL</a> HYPOTHETICAL 31.6 KD PROTEIN F54F2.9 I... <a href = #465911> 42</a> 0.001
<a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=02494160&dopt=GenPept">sp|Q61712|MTJ1_MOUSE</a> DNAJ PROTEIN HOMOLOG MTJ1 <a href = #2494160> 37</a> 0.033
<a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=01730688&dopt=GenPept">sp|P53745|YN8X_YEAST</a> HYPOTHETICAL 68.1 KD PROTEIN IN BIO3-F... <a href = #1730688> 29</a> 7.4
<PRE>
<a name = 1731448> </a><a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=01731448&dopt=GenPept">sp|P54103|ZRF1_MOUSE</a> ZUOTIN RELATED FACTOR-1
Length = 514
Score = 86.6 bits (211), Expect = 3e-17
Identities = 41/47 (87%), Positives = 44/47 (93%)
Frame = +1
Query: 1 DLQLLIKAVNLFPAGTNSRWEVIANYMNIHSSSGVKRTAKDVIGKAK 141
DLQLLIKAVNLFPAG NSRW+VIANYMNIHSSSGVKRTAKDVI + +
Sbjct: 458 DLQLLIKAVNLFPAGRNSRWDVIANYMNIHSSSGVKRTAKDVISEVR 504
</PRE>
<PRE>
<a name = 465911> </a><a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=00465911&dopt=GenPept">sp|P34454|YMA9_CAEEL</a> HYPOTHETICAL 31.6 KD PROTEIN F54F2.9 IN CHROMOSOME III
Length = 275
Score = 41.8 bits (96), Expect = 0.001
Identities = 30/122 (24%), Positives = 54/122 (43%)
Frame = +1
Query: 34 FPAGTNSRWEVIANYMNIHSSSGVKRTAKDVIGKAKSLQKLDPHQKDDINKKAFDKFKKE 213
+PAGT +RWE + +N R+A+DVI A ++++ +++D K ++
Sbjct: 159 YPAGTPNRWEQMGRVLN--------RSAEDVIAMAGKMKQM---KQEDYTKLLMTTIQQS 207
Query: 214 HGVVPQADNATPSERFXGPYTDFTPXTTEXQKLXEQALNTYPVNTXERWXXIAVAVPGRX 393
V ++++ + QK E AL YP T ERW I+ + +
Sbjct: 208 VPVEEKSED---------------DWSQAEQKAFETALQKYPKGTDERWERISEEIGSKT 252
Query: 394 KE 399
K+
Sbjct: 253 KK 254
</PRE>
<PRE>
<a name = 2494160> </a><a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=02494160&dopt=GenPept">sp|Q61712|MTJ1_MOUSE</a> DNAJ PROTEIN HOMOLOG MTJ1
Length = 552
Score = 36.7 bits (83), Expect = 0.033
Identities = 17/36 (47%), Positives = 19/36 (52%)
Frame = +1
Query: 292 TTEXQKLXEQALNTYPVNTXERWXXIAVAVPGRXKE 399
T QKL E AL YP +RW IA VP + KE
Sbjct: 496 TQSQQKLLELALQQYPKGASDRWDKIAKCVPSKSKE 531
</PRE>
<PRE>
Score = 31.3 bits (69), Expect = 1.5
Identities = 18/50 (36%), Positives = 26/50 (52%)
Frame = +1
Query: 1 DLQLLIKAVNLFPAGTNSRWEVIANYMNIHSSSGVKRTAKDVIGKAKSLQ 150
DL L +++ FP GT RW+ IA+ + R+ DV KAK L+
Sbjct: 332 DLSQLTRSMVKFPGGTPGRWDKIAHELG--------RSVTDVTTKAKELK 373
</PRE>
<PRE>
<a name = 1730688> </a><a href="http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=Protein&list_uids=01730688&dopt=GenPept">sp|P53745|YN8X_YEAST</a> HYPOTHETICAL 68.1 KD PROTEIN IN BIO3-FRE4 INTERGENIC REGION
Length = 580
Score = 29.0 bits (63), Expect = 7.4
Identities = 27/99 (27%), Positives = 41/99 (41%)
Frame = +1
Query: 52 SRWEVIANYMNIHSSSGVKRTAKDVIGKAKSLQKLDPHQKDDINKKAFDKFKKEHGVVPQ 231
+RW+ +Y V R+ KDV ++SL LD +QK +A H +
Sbjct: 75 NRWKSFISY--------VTRSRKDVKTVSRSLSNLDLYQKCSKEIRADQDISLLHSI--- 123
Query: 232 ADNATPSERFXGPYTDFTPXTTEXQKLXEQALNTYPVNT 348
E PY +FT + EQ+ N +PV+T
Sbjct: 124 -------ETKLFPYINFTALNS------EQSHNFWPVHT 149
</PRE>
<PRE>
Database: Non-redundant SwissProt sequences
Posted date: Dec 28, 1999 4:33 PM
Number of letters in database: 29,652,561
Number of sequences in database: 82,258
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.270 0.0470 0.230
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23174157
Number of Sequences: 82258
Number of extensions: 387821
Number of successful extensions: 980
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 973
Number of HSP's gapped (non-prelim): 7
length of query: 185
length of database: 29,652,561
effective HSP length: 49
effective length of query: 135
effective length of database: 25,621,919
effective search space: 3458959065
effective search space used: 3458959065
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.8 bits)
X3: 64 (24.9 bits)
S1: 41 (21.7 bits)
S2: 62 (28.6 bits)
</PRE>
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