# EPRIMER3 RESULTS FOR NirK00001

# FORWARD PRIMER STATISTICS:
# considered 87313
# GC content failed 2766
# low tm 4300
# high tm 71893
# high any compl 40
# high end compl 740
# long poly-x seq 22
#high 3' stability 1345
# ok 6207

# REVERSE PRIMER STATISTICS:
# considered 87569
# GC content failed 2773
# low tm 4326
# high tm 72012
# high any compl 42
# high end compl 581
# long poly-x seq 22
#high 3' stability 1314
# ok 6499

# PRIMER PAIR STATISTICS:
# considered 2001307
# unacceptable product size 1993131
# high any compl 16
# high end compl 752
# no internal oligo 13
# ok 7395

# INTERNAL OLIGO STATISTICS:
# considered 99642
# GC content failed 2773
# low tm 4336
# high tm 82802
# long poly-x seq 22
# ok 9709

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 146
     FORWARD PRIMER    6708   20  59.99  50.00  ATACCGGGACAAGCACTTTG

     REVERSE PRIMER    6834   20  60.13  55.00  GTTCGCCGTCTTCTTCTCTG

     INTERNAL OLIGO    6786   20  60.74  50.00  CCAGAAACGAGCAATCGATC


   2 PRODUCT SIZE: 145
     FORWARD PRIMER    7389   20  60.10  55.00  ACGCCATAGACTCCCTCCTT

     REVERSE PRIMER    7514   20  60.01  55.00  GTGACACCATCACCTTCGTG

     INTERNAL OLIGO    7427   20  60.26  50.00  CTGTTCGTTCATCTTGCCCT


   3 PRODUCT SIZE: 143
     FORWARD PRIMER    6911   20  59.99  55.00  CGACCTGTCCAGTTCGGTAT

     REVERSE PRIMER    7034   20  60.11  50.00  TGGCTACCGCTATTTTACGG

     INTERNAL OLIGO    6968   20  59.97  50.00  TAGCCGAACAGCATCTCATG


   4 PRODUCT SIZE: 145
     FORWARD PRIMER    7112   20  59.79  50.00  TAGGATAGAAGCGCCGGATA

     REVERSE PRIMER    7237   20  59.97  50.00  AGTTCCCACGAATTGACAGG

     INTERNAL OLIGO    7149   20  60.59  50.00  GCGCTATTGCCATTATCGTC


   5 PRODUCT SIZE: 149
     FORWARD PRIMER    8499   20  60.09  55.00  GAGGAACAGGGTCTGGTCAA

     REVERSE PRIMER    8628   20  60.42  60.00  CTCGCTCGAGACCTGAAGAG

     INTERNAL OLIGO    8585   20  59.87  60.00  GTCCAAGACCTAGCTGGTGC




# EPRIMER3 RESULTS FOR NirK00002

# FORWARD PRIMER STATISTICS:
# considered 88668
# GC content failed 3605
# low tm 2712
# high tm 75304
# high any compl 61
# high end compl 519
# long poly-x seq 21
#high 3' stability 1180
# ok 5266

# REVERSE PRIMER STATISTICS:
# considered 88483
# GC content failed 3502
# low tm 2708
# high tm 75226
# high any compl 47
# high end compl 519
# long poly-x seq 22
#high 3' stability 1197
# ok 5262

# PRIMER PAIR STATISTICS:
# considered 1575212
# unacceptable product size 1569195
# high any compl 27
# high end compl 556
# no internal oligo 60
# ok 5374

# INTERNAL OLIGO STATISTICS:
# considered 99648
# GC content failed 3605
# low tm 2715
# high tm 85210
# high any compl 6
# long poly-x seq 22
# ok 8090

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 149
     FORWARD PRIMER    4623   20  60.25  50.00  GCCGAAGGTCGTAAAGTTCA

     REVERSE PRIMER    4752   20  60.02  55.00  GCGTCAGCTCCACATAGTCA

     INTERNAL OLIGO    4646   20  60.19  55.00  TGACCATCCAGGAGAAGGAG


   2 PRODUCT SIZE: 150
     FORWARD PRIMER    2605   20  59.70  55.00  GCCTGCTTTACCCAGATCAC

     REVERSE PRIMER    2735   20  59.97  55.00  GACCGACATGTCCCTTGTCT

     INTERNAL OLIGO    2626   20  59.83  50.00  TCGTCGAACAGGAGTTCATG


   3 PRODUCT SIZE: 147
     FORWARD PRIMER    4646   20  60.19  55.00  TGACCATCCAGGAGAAGGAG

     REVERSE PRIMER    4773   20  60.15  55.00  CGTTGGTCTCAGGGTTGACT

     INTERNAL OLIGO    4752   20  60.02  55.00  TGACTATGTGGAGCTGACGC


   4 PRODUCT SIZE: 145
     FORWARD PRIMER    1217   20  59.93  50.00  CGTGTCCTTCATGGATTCCT

     REVERSE PRIMER    1342   20  60.22  50.00  CAAGAACATCGCCCTCATCT

     INTERNAL OLIGO    1260   19  59.90  57.90  GGGCCGAGATAGGTCACAT


   5 PRODUCT SIZE: 150
     FORWARD PRIMER     981   20  59.60  45.00  AGCGAGTTGCCCATATTGTT

     REVERSE PRIMER    1111   20  59.85  50.00  CGTGCCGGTCTATAATGGTT

     INTERNAL OLIGO    1069   20  59.84  55.00  GAAAGTCCGCCAGTATCTGC




# EPRIMER3 RESULTS FOR NirK00003

# FORWARD PRIMER STATISTICS:
# considered 87331
# GC content failed 3312
# low tm 4606
# high tm 70606
# high any compl 100
# high end compl 551
# long poly-x seq 96
#high 3' stability 1288
# ok 6772

# REVERSE PRIMER STATISTICS:
# considered 87147
# GC content failed 3324
# low tm 4605
# high tm 70441
# high any compl 97
# high end compl 482
# long poly-x seq 95
#high 3' stability 1316
# ok 6787

# PRIMER PAIR STATISTICS:
# considered 1642899
# unacceptable product size 1635653
# high any compl 9
# high end compl 676
# no internal oligo 7
# ok 6554

# INTERNAL OLIGO STATISTICS:
# considered 99153
# GC content failed 3314
# low tm 4609
# high tm 81068
# high any compl 42
# long poly-x seq 98
# ok 10022

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 148
     FORWARD PRIMER    8290   20  60.14  50.00  GACTATGTTGCGGCCAAGTT

     REVERSE PRIMER    8418   20  60.01  50.00  GACGAAACACGAACCACCTT

     INTERNAL OLIGO    8376   20  60.18  50.00  TCATGGCTTCAGCCCTTATC


   2 PRODUCT SIZE: 148
     FORWARD PRIMER    8016   20  59.84  40.00  TTAATTCGCAATGTGCTTCG

     REVERSE PRIMER    8144   20  59.99  50.00  TATGTGCCGGGTGTTGAGTA

     INTERNAL OLIGO    8122   20  59.87  60.00  GTAGGTCAGCCGGACTTCTG


   3 PRODUCT SIZE: 150
     FORWARD PRIMER    8122   20  59.87  60.00  GTAGGTCAGCCGGACTTCTG

     REVERSE PRIMER    8252   20  60.16  55.00  GATGTATCCGTGGAGGATGG

     INTERNAL OLIGO    8144   20  59.99  50.00  TACTCAACACCCGGCACATA


   4 PRODUCT SIZE: 147
     FORWARD PRIMER    8291   20  60.14  50.00  ACTATGTTGCGGCCAAGTTC

     REVERSE PRIMER    8418   20  60.01  50.00  GACGAAACACGAACCACCTT

     INTERNAL OLIGO    8376   20  60.18  50.00  TCATGGCTTCAGCCCTTATC


   5 PRODUCT SIZE: 147
     FORWARD PRIMER    8290   20  60.14  50.00  GACTATGTTGCGGCCAAGTT

     REVERSE PRIMER    8417   20  60.01  50.00  ACGAAACACGAACCACCTTC

     INTERNAL OLIGO    8376   20  60.18  50.00  TCATGGCTTCAGCCCTTATC




# EPRIMER3 RESULTS FOR NirK00004

# FORWARD PRIMER STATISTICS:
# considered 90150
# GC content failed 6237
# low tm 2247
# high tm 76114
# high any compl 84
# high end compl 398
# long poly-x seq 46
#high 3' stability 986
# ok 4038

# REVERSE PRIMER STATISTICS:
# considered 90580
# GC content failed 6342
# low tm 2247
# high tm 76409
# high any compl 67
# high end compl 368
# long poly-x seq 45
#high 3' stability 937
# ok 4165

# PRIMER PAIR STATISTICS:
# considered 1045647
# unacceptable product size 1042092
# high any compl 16
# high end compl 275
# no internal oligo 32
# ok 3232

# INTERNAL OLIGO STATISTICS:
# considered 99481
# GC content failed 6418
# low tm 2247
# high tm 84429
# high any compl 3
# long poly-x seq 46
# ok 6338

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 143
     FORWARD PRIMER    4901   20  60.18  55.00  GGCGTGTTCGTGTATCACTG

     REVERSE PRIMER    5024   20  60.01  60.00  TAGACCCGGTCGTACCGTAG

     INTERNAL OLIGO    4952   18  60.21  61.11  GTCGTCTCCGGCATGAAC


   2 PRODUCT SIZE: 150
     FORWARD PRIMER    5418   20  59.48  50.00  ACGCCTACCTCAACCACAAT

     REVERSE PRIMER    5548   20  60.18  55.00  ACGTGGCGTCTTAGTTGGTC

     INTERNAL OLIGO    5499   20  60.27  55.00  ACGACCTGATGAAGCAGGTC


   3 PRODUCT SIZE: 149
     FORWARD PRIMER    4629   20  59.50  45.00  TCGAATTCAAGATGGTGGTG

     REVERSE PRIMER    4758   20  59.83  50.00  GGGATTGACCAACGTCAGTT

     INTERNAL OLIGO    4654   20  60.11  50.00  AAAGGAAGTGGTGATCGACG


   4 PRODUCT SIZE: 149
     FORWARD PRIMER    5419   20  60.52  55.00  CGCCTACCTCAACCACAATC

     REVERSE PRIMER    5548   20  60.18  55.00  ACGTGGCGTCTTAGTTGGTC

     INTERNAL OLIGO    5499   20  60.27  55.00  ACGACCTGATGAAGCAGGTC


   5 PRODUCT SIZE: 145
     FORWARD PRIMER    3190   20  60.10  50.00  CTGACCGATATTCCGCTTGT

     REVERSE PRIMER    3315   20  59.60  45.00  AGTTTCGCAACCATGGAAGT

     INTERNAL OLIGO    3250   20  60.74  50.00  GACAATCGCGATTTAGCTGG




# EPRIMER3 RESULTS FOR NirK00005

# FORWARD PRIMER STATISTICS:
# considered 87251
# GC content failed 3476
# low tm 4270
# high tm 71031
# high any compl 97
# high end compl 737
#high 3' stability 1379
# ok 6261

# REVERSE PRIMER STATISTICS:
# considered 87244
# GC content failed 3476
# low tm 4373
# high tm 70845
# high any compl 84
# high end compl 587
#high 3' stability 1372
# ok 6507

# PRIMER PAIR STATISTICS:
# considered 2030304
# unacceptable product size 2022508
# high any compl 41
# high end compl 687
# no internal oligo 10
# ok 7058

# INTERNAL OLIGO STATISTICS:
# considered 99795
# GC content failed 3476
# low tm 4422
# high tm 81868
# high any compl 4
# ok 10025

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 150
     FORWARD PRIMER    4223   20  60.04  45.00  TGCATGATCGATGTCTCCAT

     REVERSE PRIMER    4353   20  60.21  45.00  AGAAGAAAGCCGACGGAAAT

     INTERNAL OLIGO    4276   20  60.02  50.00  GCAGTCGTTGAGATTACGCA


   2 PRODUCT SIZE: 150
     FORWARD PRIMER    1805   20  59.99  60.00  GTCTGTCCCAGCTCCTGAAG

     REVERSE PRIMER    1935   20  60.37  55.00  CGACGGCGAGGTAGAAGATA

     INTERNAL OLIGO    1832   20  59.80  55.00  CTTTCCTGACCTGGATCGAG


   3 PRODUCT SIZE: 147
     FORWARD PRIMER    4223   20  60.04  45.00  TGCATGATCGATGTCTCCAT

     REVERSE PRIMER    4350   20  60.21  45.00  AGAAAGCCGACGGAAATCTT

     INTERNAL OLIGO    4276   20  60.02  50.00  GCAGTCGTTGAGATTACGCA


   4 PRODUCT SIZE: 144
     FORWARD PRIMER    4547   20  59.87  50.00  TCAAGACTGCCAAACAGGTG

     REVERSE PRIMER    4671   20  59.96  50.00  CCTGGATGGTTAGCGTGAAT

     INTERNAL OLIGO    4624   20  59.95  50.00  GTCCATGCTCATCAGCAGAA


   5 PRODUCT SIZE: 149
     FORWARD PRIMER    4221   20  59.75  50.00  TCTGCATGATCGATGTCTCC

     REVERSE PRIMER    4350   20  60.21  45.00  AGAAAGCCGACGGAAATCTT

     INTERNAL OLIGO    4276   20  60.02  50.00  GCAGTCGTTGAGATTACGCA




# EPRIMER3 RESULTS FOR NirK00006

# FORWARD PRIMER STATISTICS:
# considered 85867
# GC content failed 1287
# low tm 7342
# high tm 65768
# high any compl 128
# high end compl 876
# long poly-x seq 128
#high 3' stability 1473
# ok 8865

# REVERSE PRIMER STATISTICS:
# considered 85290
# GC content failed 1272
# low tm 7456
# high tm 65061
# high any compl 90
# high end compl 796
# long poly-x seq 121
#high 3' stability 1546
# ok 8948

# PRIMER PAIR STATISTICS:
# considered 2902817
# unacceptable product size 2892752
# high any compl 8
# high end compl 858
# no internal oligo 3
# ok 9196

# INTERNAL OLIGO STATISTICS:
# considered 98877
# GC content failed 1294
# low tm 7456
# high tm 76729
# high any compl 33
# long poly-x seq 132
# ok 13233

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 149
     FORWARD PRIMER    8918   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     REVERSE PRIMER    9047   20  60.07  45.00  GCCTGGTCTGGCATTAAAAA

     INTERNAL OLIGO    8943   20  60.05  55.00  GGAGGAGTGTCATGAAGGGA


   2 PRODUCT SIZE: 148
     FORWARD PRIMER    9844   20  60.05  55.00  CGAGACCACGCTCTACAACA

     REVERSE PRIMER    9972   20  59.90  50.00  AAGCTTCTTGCAGCCTTCAG

     INTERNAL OLIGO    9898   20  59.96  50.00  GGCTGAAAATCTCAAGGCTG


   3 PRODUCT SIZE: 150
     FORWARD PRIMER    9043   20  60.07  45.00  TGGCTTTTTAATGCCAGACC

     REVERSE PRIMER    9173   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     INTERNAL OLIGO    9127   20  59.89  55.00  CGAGAGCTGCAAGGTTCTCT


   4 PRODUCT SIZE: 149
     FORWARD PRIMER    9044   20  60.07  45.00  GGCTTTTTAATGCCAGACCA

     REVERSE PRIMER    9173   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     INTERNAL OLIGO    9127   20  59.89  55.00  CGAGAGCTGCAAGGTTCTCT


   5 PRODUCT SIZE: 150
     FORWARD PRIMER    8918   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     REVERSE PRIMER    9048   20  59.71  45.00  AGCCTGGTCTGGCATTAAAA

     INTERNAL OLIGO    8943   20  60.05  55.00  GGAGGAGTGTCATGAAGGGA




# EPRIMER3 RESULTS FOR NirK00007

# FORWARD PRIMER STATISTICS:
# considered 85153
# GC content failed 1250
# low tm 7464
# high tm 64879
# high any compl 96
# high end compl 843
# long poly-x seq 122
#high 3' stability 1562
# ok 8937

# REVERSE PRIMER STATISTICS:
# considered 85748
# GC content failed 1265
# low tm 7347
# high tm 65616
# high any compl 112
# high end compl 760
# long poly-x seq 129
#high 3' stability 1487
# ok 9032

# PRIMER PAIR STATISTICS:
# considered 2871561
# unacceptable product size 2861494
# high any compl 7
# high end compl 852
# no internal oligo 3
# ok 9205

# INTERNAL OLIGO STATISTICS:
# considered 98869
# GC content failed 1272
# low tm 7464
# high tm 76670
# high any compl 50
# long poly-x seq 133
# ok 13280

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 149
     FORWARD PRIMER     935   20  60.07  45.00  GCCTGGTCTGGCATTAAAAA

     REVERSE PRIMER    1064   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     INTERNAL OLIGO    1039   20  60.05  55.00  TCCCTTCATGACACTCCTCC


   2 PRODUCT SIZE: 148
     FORWARD PRIMER      10   20  59.90  50.00  AAGCTTCTTGCAGCCTTCAG

     REVERSE PRIMER     138   20  60.05  55.00  CGAGACCACGCTCTACAACA

     INTERNAL OLIGO      84   20  59.96  50.00  CAGCCTTGAGATTTTCAGCC


   3 PRODUCT SIZE: 150
     FORWARD PRIMER     809   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     REVERSE PRIMER     939   20  60.07  45.00  TGGCTTTTTAATGCCAGACC

     INTERNAL OLIGO     855   20  59.89  55.00  AGAGAACCTTGCAGCTCTCG


   4 PRODUCT SIZE: 149
     FORWARD PRIMER     809   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     REVERSE PRIMER     938   20  60.07  45.00  GGCTTTTTAATGCCAGACCA

     INTERNAL OLIGO     855   20  59.89  55.00  AGAGAACCTTGCAGCTCTCG


   5 PRODUCT SIZE: 150
     FORWARD PRIMER     934   20  59.71  45.00  AGCCTGGTCTGGCATTAAAA

     REVERSE PRIMER    1064   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     INTERNAL OLIGO    1039   20  60.05  55.00  TCCCTTCATGACACTCCTCC




# EPRIMER3 RESULTS FOR NirK00008

# FORWARD PRIMER STATISTICS:
# considered 85199
# GC content failed 1188
# low tm 7566
# high tm 64875
# high any compl 100
# high end compl 846
# long poly-x seq 125
#high 3' stability 1558
# ok 8941

# REVERSE PRIMER STATISTICS:
# considered 85783
# GC content failed 1203
# low tm 7452
# high tm 65587
# high any compl 111
# high end compl 769
# long poly-x seq 128
#high 3' stability 1485
# ok 9048

# PRIMER PAIR STATISTICS:
# considered 2844588
# unacceptable product size 2834600
# high any compl 7
# high end compl 802
# no internal oligo 3
# ok 9176

# INTERNAL OLIGO STATISTICS:
# considered 98877
# GC content failed 1210
# low tm 7566
# high tm 76619
# high any compl 52
# long poly-x seq 132
# ok 13298

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 149
     FORWARD PRIMER     936   20  60.07  45.00  GCCTGGTCTGGCATTAAAAA

     REVERSE PRIMER    1065   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     INTERNAL OLIGO    1040   20  60.05  55.00  TCCCTTCATGACACTCCTCC


   2 PRODUCT SIZE: 148
     FORWARD PRIMER      11   20  59.90  50.00  AAGCTTCTTGCAGCCTTCAG

     REVERSE PRIMER     139   20  60.05  55.00  CGAGACCACGCTCTACAACA

     INTERNAL OLIGO      85   20  59.96  50.00  CAGCCTTGAGATTTTCAGCC


   3 PRODUCT SIZE: 150
     FORWARD PRIMER     810   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     REVERSE PRIMER     940   20  60.07  45.00  TGGCTTTTTAATGCCAGACC

     INTERNAL OLIGO     856   20  59.89  55.00  AGAGAACCTTGCAGCTCTCG


   4 PRODUCT SIZE: 149
     FORWARD PRIMER     810   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     REVERSE PRIMER     939   20  60.07  45.00  GGCTTTTTAATGCCAGACCA

     INTERNAL OLIGO     856   20  59.89  55.00  AGAGAACCTTGCAGCTCTCG


   5 PRODUCT SIZE: 150
     FORWARD PRIMER     935   20  59.71  45.00  AGCCTGGTCTGGCATTAAAA

     REVERSE PRIMER    1065   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     INTERNAL OLIGO    1040   20  60.05  55.00  TCCCTTCATGACACTCCTCC




# EPRIMER3 RESULTS FOR NirK00009

# FORWARD PRIMER STATISTICS:
# considered 85199
# GC content failed 1188
# low tm 7566
# high tm 64875
# high any compl 100
# high end compl 846
# long poly-x seq 125
#high 3' stability 1558
# ok 8941

# REVERSE PRIMER STATISTICS:
# considered 85783
# GC content failed 1203
# low tm 7452
# high tm 65587
# high any compl 111
# high end compl 769
# long poly-x seq 128
#high 3' stability 1485
# ok 9048

# PRIMER PAIR STATISTICS:
# considered 2844588
# unacceptable product size 2834600
# high any compl 7
# high end compl 802
# no internal oligo 3
# ok 9176

# INTERNAL OLIGO STATISTICS:
# considered 98877
# GC content failed 1210
# low tm 7566
# high tm 76619
# high any compl 52
# long poly-x seq 132
# ok 13298

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 149
     FORWARD PRIMER     936   20  60.07  45.00  GCCTGGTCTGGCATTAAAAA

     REVERSE PRIMER    1065   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     INTERNAL OLIGO    1040   20  60.05  55.00  TCCCTTCATGACACTCCTCC


   2 PRODUCT SIZE: 148
     FORWARD PRIMER      11   20  59.90  50.00  AAGCTTCTTGCAGCCTTCAG

     REVERSE PRIMER     139   20  60.05  55.00  CGAGACCACGCTCTACAACA

     INTERNAL OLIGO      85   20  59.96  50.00  CAGCCTTGAGATTTTCAGCC


   3 PRODUCT SIZE: 150
     FORWARD PRIMER     810   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     REVERSE PRIMER     940   20  60.07  45.00  TGGCTTTTTAATGCCAGACC

     INTERNAL OLIGO     856   20  59.89  55.00  AGAGAACCTTGCAGCTCTCG


   4 PRODUCT SIZE: 149
     FORWARD PRIMER     810   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     REVERSE PRIMER     939   20  60.07  45.00  GGCTTTTTAATGCCAGACCA

     INTERNAL OLIGO     856   20  59.89  55.00  AGAGAACCTTGCAGCTCTCG


   5 PRODUCT SIZE: 150
     FORWARD PRIMER     935   20  59.71  45.00  AGCCTGGTCTGGCATTAAAA

     REVERSE PRIMER    1065   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     INTERNAL OLIGO    1040   20  60.05  55.00  TCCCTTCATGACACTCCTCC




# EPRIMER3 RESULTS FOR NirK00010

# FORWARD PRIMER STATISTICS:
# considered 85959
# GC content failed 1273
# low tm 7643
# high tm 65398
# high any compl 130
# high end compl 887
# long poly-x seq 103
#high 3' stability 1483
# ok 9042

# REVERSE PRIMER STATISTICS:
# considered 85404
# GC content failed 1251
# low tm 8174
# high tm 64238
# high any compl 86
# high end compl 792
# long poly-x seq 99
#high 3' stability 1551
# ok 9213

# PRIMER PAIR STATISTICS:
# considered 2884339
# unacceptable product size 2874225
# high any compl 8
# high end compl 833
# ok 9273

# INTERNAL OLIGO STATISTICS:
# considered 99030
# GC content failed 1273
# low tm 8174
# high tm 75932
# high any compl 25
# long poly-x seq 110
# ok 13516

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 149
     FORWARD PRIMER    8808   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     REVERSE PRIMER    8937   20  60.07  45.00  GCCTGGTCTGGCATTAAAAA

     INTERNAL OLIGO    8833   20  60.05  55.00  GGAGGAGTGTCATGAAGGGA


   2 PRODUCT SIZE: 148
     FORWARD PRIMER    9716   20  60.05  55.00  CGAGACCACGCTCTACAACA

     REVERSE PRIMER    9844   20  59.90  50.00  AAGCTTCTTGCAGCCTTCAG

     INTERNAL OLIGO    9770   20  59.96  50.00  GGCTGAAAATCTCAAGGCTG


   3 PRODUCT SIZE: 150
     FORWARD PRIMER    8933   20  60.07  45.00  TGGCTTTTTAATGCCAGACC

     REVERSE PRIMER    9063   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     INTERNAL OLIGO    9017   20  59.89  55.00  CGAGAGCTGCAAGGTTCTCT


   4 PRODUCT SIZE: 149
     FORWARD PRIMER    8934   20  60.07  45.00  GGCTTTTTAATGCCAGACCA

     REVERSE PRIMER    9063   20  59.79  45.00  TTGAACTGTTGCTGCTGCTT

     INTERNAL OLIGO    9017   20  59.89  55.00  CGAGAGCTGCAAGGTTCTCT


   5 PRODUCT SIZE: 150
     FORWARD PRIMER    8808   20  60.10  50.00  CCGGCACAAGAATAGACGAT

     REVERSE PRIMER    8938   20  59.71  45.00  AGCCTGGTCTGGCATTAAAA

     INTERNAL OLIGO    8833   20  60.05  55.00  GGAGGAGTGTCATGAAGGGA




# EPRIMER3 RESULTS FOR NirK00011

# FORWARD PRIMER STATISTICS:
# considered 89983
# GC content failed 6442
# low tm 3302
# high tm 73164
# high any compl 79
# high end compl 613
# long poly-x seq 21
#high 3' stability 1037
# ok 5325

# REVERSE PRIMER STATISTICS:
# considered 89739
# GC content failed 6442
# low tm 3317
# high tm 72931
# high any compl 60
# high end compl 490
# long poly-x seq 19
#high 3' stability 1068
# ok 5412

# PRIMER PAIR STATISTICS:
# considered 2029923
# unacceptable product size 2021914
# high any compl 101
# high end compl 940
# no internal oligo 29
# ok 6939

# INTERNAL OLIGO STATISTICS:
# considered 99686
# GC content failed 6468
# low tm 3317
# high tm 81835
# high any compl 18
# long poly-x seq 21
# ok 8027

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 146
     FORWARD PRIMER    4625   20  60.16  55.00  GTGGAGTTCAAGCTGGTGGT

     REVERSE PRIMER    4751   20  59.99  50.00  TTGATCAGCGTCAGTTCGAC

     INTERNAL OLIGO    4731   20  59.86  55.00  TCGTGCATCAGGACGACTAC


   2 PRODUCT SIZE: 149
     FORWARD PRIMER    6301   20  60.29  55.00  CAGTATCGGTAGCCGCTTGT

     REVERSE PRIMER    6430   20  59.84  55.00  CAAATAGGGCACAGGCTCTC

     INTERNAL OLIGO    6325   20  58.62  45.00  ATTGTTGACGTTGAAGGTCG


   3 PRODUCT SIZE: 148
     FORWARD PRIMER    6301   20  60.29  55.00  CAGTATCGGTAGCCGCTTGT

     REVERSE PRIMER    6429   20  59.84  50.00  AAATAGGGCACAGGCTCTCA

     INTERNAL OLIGO    6325   20  58.62  45.00  ATTGTTGACGTTGAAGGTCG


   4 PRODUCT SIZE: 144
     FORWARD PRIMER    8972   20  60.37  50.00  GCAGCTGTTCGATCAGGAAT

     REVERSE PRIMER    9096   20  60.00  55.00  GTCTCGGCGAAGAAGTTGTC

     INTERNAL OLIGO    9030   20  59.86  50.00  AAGATCATCCCGGAGGAGAT


   5 PRODUCT SIZE: 142
     FORWARD PRIMER    6308   20  60.14  50.00  GGTAGCCGCTTGTTGACATT

     REVERSE PRIMER    6430   20  59.84  55.00  CAAATAGGGCACAGGCTCTC

     INTERNAL OLIGO    6332   19  60.82  52.63  ACGTTGAAGGTCGCGATCT




# EPRIMER3 RESULTS FOR NirK00012

# FORWARD PRIMER STATISTICS:
# considered 90415
# GC content failed 5828
# low tm 2402
# high tm 76341
# high any compl 73
# high end compl 348
# long poly-x seq 24
#high 3' stability 977
# ok 4422

# REVERSE PRIMER STATISTICS:
# considered 90692
# GC content failed 5866
# low tm 2396
# high tm 76578
# high any compl 57
# high end compl 335
# long poly-x seq 23
#high 3' stability 947
# ok 4490

# PRIMER PAIR STATISTICS:
# considered 993874
# unacceptable product size 990489
# high any compl 7
# high end compl 335
# no internal oligo 43
# ok 3000

# INTERNAL OLIGO STATISTICS:
# considered 99631
# GC content failed 5870
# low tm 2420
# high tm 84584
# high any compl 3
# long poly-x seq 24
# ok 6730

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 147
     FORWARD PRIMER    2867   20  60.02  55.00  GGCACGTGCAGATAGAGTGA

     REVERSE PRIMER    2994   20  60.15  50.00  AGTGAGCTACGGCGAAGAAA

     INTERNAL OLIGO    2969   20  60.00  60.00  GGATAGCTCGTGTAGCGAGG


   2 PRODUCT SIZE: 146
     FORWARD PRIMER    2867   20  60.02  55.00  GGCACGTGCAGATAGAGTGA

     REVERSE PRIMER    2993   20  60.15  55.00  GTGAGCTACGGCGAAGAAAG

     INTERNAL OLIGO    2969   20  60.00  60.00  GGATAGCTCGTGTAGCGAGG


   3 PRODUCT SIZE: 146
     FORWARD PRIMER    8657   20  59.91  45.00  TATTGGCGGCGATTATTAGG

     REVERSE PRIMER    8783   20  60.11  45.00  CGCAACTTGATGGGTTTTCT

     INTERNAL OLIGO    8693   20  59.81  50.00  CGTCAATGATACGGGGTTCT


   4 PRODUCT SIZE: 149
     FORWARD PRIMER    5297   20  59.69  60.00  GCGACTATGTCTGGGAGACC

     REVERSE PRIMER    5426   20  59.82  50.00  CTCGATCAGGTTATGCGTGA

     INTERNAL OLIGO    5382   20  59.87  50.00  GGCTGCGATGTACAAGTTCA


   5 PRODUCT SIZE: 142
     FORWARD PRIMER    2867   20  60.02  55.00  GGCACGTGCAGATAGAGTGA

     REVERSE PRIMER    2989   20  60.15  55.00  GCTACGGCGAAGAAAGACTG

     INTERNAL OLIGO    2969   20  60.00  60.00  GGATAGCTCGTGTAGCGAGG




# EPRIMER3 RESULTS FOR NirK00013

# FORWARD PRIMER STATISTICS:
# considered 87074
# GC content failed 3447
# low tm 4188
# high tm 71171
# high any compl 85
# high end compl 754
# long poly-x seq 43
#high 3' stability 1359
# ok 6027

# REVERSE PRIMER STATISTICS:
# considered 87306
# GC content failed 3420
# low tm 4123
# high tm 71502
# high any compl 95
# high end compl 748
# long poly-x seq 43
#high 3' stability 1334
# ok 6041

# PRIMER PAIR STATISTICS:
# considered 2359499
# unacceptable product size 2351372
# high any compl 59
# high end compl 834
# ok 7234

# INTERNAL OLIGO STATISTICS:
# considered 99489
# GC content failed 3449
# low tm 4195
# high tm 82287
# long poly-x seq 44
# ok 9514

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 148
     FORWARD PRIMER    4368   20  59.84  55.00  GAACAGGCAAAGAGGAGGTG

     REVERSE PRIMER    4496   20  60.14  50.00  GAGCATGTTTCGCTCTGTCA

     INTERNAL OLIGO    4476   20  60.92  55.00  CTCCTCCGCTACAAATGCTG


   2 PRODUCT SIZE: 142
     FORWARD PRIMER    1422   20  60.22  50.00  TCTGGACGTGGAAAAGGAAG

     REVERSE PRIMER    1544   20  59.89  45.00  CATCGGTTCATCGAAATCCT

     INTERNAL OLIGO    1474   20  60.12  45.00  TGCGGTGACATTCACTCAAT


   3 PRODUCT SIZE: 147
     FORWARD PRIMER    9699   20  60.33  55.00  GGGAAGCTCCTGGGTAATGT

     REVERSE PRIMER    9826   20  59.72  45.00  TTGGATATTCTTCCCGATGG

     INTERNAL OLIGO    9780   20  60.23  50.00  GACATCCTGCTCGACGAAAT


   4 PRODUCT SIZE: 144
     FORWARD PRIMER    2755   20  59.61  55.00  CTGATCCGGCCATCTATCTC

     REVERSE PRIMER    2879   20  60.07  55.00  GCTCCGGGTAAAGAAAGGTC

     INTERNAL OLIGO    2816   20  59.81  50.00  GCTTAAATCGCTGGATCGTC


   5 PRODUCT SIZE: 138
     FORWARD PRIMER    4368   20  59.84  55.00  GAACAGGCAAAGAGGAGGTG

     REVERSE PRIMER    4486   20  60.06  50.00  CGCTCTGTCACAGCATTTGT

     INTERNAL OLIGO    4442   21  59.87  42.86  AGCGAAGTTTGTTCCATCTCA




# EPRIMER3 RESULTS FOR NirK00014

# FORWARD PRIMER STATISTICS:
# considered 87323
# GC content failed 3420
# low tm 4119
# high tm 71497
# high any compl 103
# high end compl 836
# long poly-x seq 43
#high 3' stability 1333
# ok 5972

# REVERSE PRIMER STATISTICS:
# considered 87004
# GC content failed 3418
# low tm 4122
# high tm 71241
# high any compl 75
# high end compl 709
# long poly-x seq 43
#high 3' stability 1366
# ok 6030

# PRIMER PAIR STATISTICS:
# considered 2341928
# unacceptable product size 2333905
# high any compl 58
# high end compl 775
# ok 7190

# INTERNAL OLIGO STATISTICS:
# considered 99489
# GC content failed 3420
# low tm 4128
# high tm 82432
# long poly-x seq 44
# ok 9465

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 148
     FORWARD PRIMER    5554   20  60.14  50.00  GAGCATGTTTCGCTCTGTCA

     REVERSE PRIMER    5682   20  59.84  55.00  GAACAGGCAAAGAGGAGGTG

     INTERNAL OLIGO    5574   20  60.92  55.00  CAGCATTTGTAGCGGAGGAG


   2 PRODUCT SIZE: 142
     FORWARD PRIMER    8506   20  59.89  45.00  CATCGGTTCATCGAAATCCT

     REVERSE PRIMER    8628   20  60.22  50.00  TCTGGACGTGGAAAAGGAAG

     INTERNAL OLIGO    8576   20  60.12  45.00  ATTGAGTGAATGTCACCGCA


   3 PRODUCT SIZE: 147
     FORWARD PRIMER     224   20  59.72  45.00  TTGGATATTCTTCCCGATGG

     REVERSE PRIMER     351   20  60.33  55.00  GGGAAGCTCCTGGGTAATGT

     INTERNAL OLIGO     270   20  60.23  50.00  ATTTCGTCGAGCAGGATGTC


   4 PRODUCT SIZE: 144
     FORWARD PRIMER    7171   20  60.07  55.00  GCTCCGGGTAAAGAAAGGTC

     REVERSE PRIMER    7295   20  59.61  55.00  CTGATCCGGCCATCTATCTC

     INTERNAL OLIGO    7234   20  59.81  50.00  GACGATCCAGCGATTTAAGC


   5 PRODUCT SIZE: 138
     FORWARD PRIMER    5564   20  60.06  50.00  CGCTCTGTCACAGCATTTGT

     REVERSE PRIMER    5682   20  59.84  55.00  GAACAGGCAAAGAGGAGGTG

     INTERNAL OLIGO    5607   21  59.87  42.86  TGAGATGGAACAAACTTCGCT




# EPRIMER3 RESULTS FOR NirK00015

# FORWARD PRIMER STATISTICS:
# considered 87748
# GC content failed 3268
# low tm 9717
# high tm 63380
# high any compl 66
# high end compl 746
# long poly-x seq 148
#high 3' stability 1245
# ok 9178

# REVERSE PRIMER STATISTICS:
# considered 88011
# GC content failed 3280
# low tm 9717
# high tm 63581
# high any compl 51
# high end compl 657
# long poly-x seq 142
#high 3' stability 1218
# ok 9365

# PRIMER PAIR STATISTICS:
# considered 2139247
# unacceptable product size 2129956
# high any compl 38
# high end compl 791
# no internal oligo 33
# ok 8429

# INTERNAL OLIGO STATISTICS:
# considered 98798
# GC content failed 3278
# low tm 9717
# high tm 72841
# high any compl 6
# long poly-x seq 150
# ok 12806

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 148
     FORWARD PRIMER    1577   20  59.98  50.00  CGGTGATGTTCAAGAGCAGA

     REVERSE PRIMER    1705   20  59.94  50.00  CATCTGCTCAACGAGATGGA

     INTERNAL OLIGO    1681   20  60.33  50.00  TACTGCAGGTGTTGTGCGAT


   2 PRODUCT SIZE: 150
     FORWARD PRIMER     955   20  59.84  45.00  GGTGGTTTTCACGAACGAAT

     REVERSE PRIMER    1085   20  59.96  55.00  GACCCTGTACGGCCTATCAA

     INTERNAL OLIGO    1037   20  59.91  45.00  TTGATGATGAGCTCGATTGC


   3 PRODUCT SIZE: 150
     FORWARD PRIMER    1758   20  60.14  55.00  AGCCCTACACCTCACAATGC

     REVERSE PRIMER    1888   20  59.85  45.00  AGCGGGAAAGAGTGTTTTCA

     INTERNAL OLIGO    1784   20  59.63  55.00  GAACAGTCTGCACGCTGTTC


   4 PRODUCT SIZE: 148
     FORWARD PRIMER    1758   20  60.14  55.00  AGCCCTACACCTCACAATGC

     REVERSE PRIMER    1886   20  59.85  50.00  CGGGAAAGAGTGTTTTCAGC

     INTERNAL OLIGO    1784   20  59.63  55.00  GAACAGTCTGCACGCTGTTC


   5 PRODUCT SIZE: 144
     FORWARD PRIMER     251   20  60.01  55.00  CTTTCGCCTCGGTGACTTAG

     REVERSE PRIMER     375   20  60.19  55.00  GAACCCGGGACCTACTGATT

     INTERNAL OLIGO     308   20  60.05  45.00  CGCGATTCCATCGCTATAAT




# EPRIMER3 RESULTS FOR NirK00016

# FORWARD PRIMER STATISTICS:
# considered 83779
# GC content failed 729
# low tm 24977
# high tm 35695
# high any compl 315
# high end compl 1320
# long poly-x seq 241
#high 3' stability 1806
# ok 18696

# REVERSE PRIMER STATISTICS:
# considered 83707
# GC content failed 731
# low tm 25014
# high tm 35609
# high any compl 244
# high end compl 1147
# long poly-x seq 223
#high 3' stability 1809
# ok 18930

# PRIMER PAIR STATISTICS:
# considered 4438939
# unacceptable product size 4421225
# high any compl 31
# high end compl 1409
# ok 16274

# INTERNAL OLIGO STATISTICS:
# considered 98090
# GC content failed 729
# low tm 25257
# high tm 46152
# high any compl 58
# long poly-x seq 247
# ok 25647

#                      Start  Len   Tm     GC%   Sequence

   1 PRODUCT SIZE: 149
     FORWARD PRIMER    6353   20  60.02  50.00  ACTCACTTCGGCTGAATGCT

     REVERSE PRIMER    6482   20  60.15  45.00  CATTTAATCCGGATGCCAAC

     INTERNAL OLIGO    6435   20  59.43  50.00  CCCACCAATATTTGGCTAGC


   2 PRODUCT SIZE: 147
     FORWARD PRIMER    6202   20  59.97  55.00  GGCGATTAGCGCTGTCTATC

     REVERSE PRIMER    6329   20  60.06  50.00  TGGCCTTTCTCTCCTCTTCA

     INTERNAL OLIGO    6252   20  60.26  50.00  AATCTTCTGTGCACCTTGCC


   3 PRODUCT SIZE: 148
     FORWARD PRIMER    6353   20  60.02  50.00  ACTCACTTCGGCTGAATGCT

     REVERSE PRIMER    6481   20  60.15  40.00  ATTTAATCCGGATGCCAACA

     INTERNAL OLIGO    6435   20  59.43  50.00  CCCACCAATATTTGGCTAGC


   4 PRODUCT SIZE: 148
     FORWARD PRIMER    4535   20  59.97  55.00  TAGGCGTATAGACCGGGTTG

     REVERSE PRIMER    4663   20  59.85  50.00  CACACATTATTGGCGGTCAC

     INTERNAL OLIGO    4636   20  60.16  50.00  TGAGCCTGTGTTCCACACAT


   5 PRODUCT SIZE: 150
     FORWARD PRIMER    7896   20  59.91  50.00  AGCAAGCTGACCACTGGTTT

     REVERSE PRIMER    8026   20  60.23  50.00  TCTGAAACCACCAAGGAAGC

     INTERNAL OLIGO    7976   20  60.10  50.00  CTATGCCCTTCTGCCACAAT