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^PLATFORM = Murine 15K long oligo array version 2.0
!Platform_title = Murine 15K long oligo array version 2.0
!Platform_technology = spotted oligonucleotide
!Platform_distribution = non-commercial
!Platform_organism = Mus musculus
!Platform_manufacturer = Un. London microarray facility
!Platform_manufacture_protocol = 1. Oligos are arrayed in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
!Platform_manufacture_protocol = 2. Resuspend oligos in water to 20 uM and rearray 5 L into 384-well, Genetix polystyrene V-bottom plates (cat# X6004).
!Platform_manufacture_protocol = 3. Allow Genetix plates to dry through passive water evaporation in a protected environment (e.g., chemical hood).
!Platform_manufacture_protocol = 4. Before printing, add 5 L of 1X Printing Buffer to each well. This can be done the night before a print run is started.
!Platform_manufacture_protocol = 5. Seal plates with Corning seals.
!Platform_manufacture_protocol = 6. Incubate at 37C for 30 minutes to aid resuspension of DNA.
!Platform_manufacture_protocol = 7. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
!Platform_manufacture_protocol = 8. Centrifuge plates at 2000 rpm for 3 minutes.
!Platform_manufacture_protocol = 9. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing. For second and third cassettes, wait until 30 minutes before next cassette is needed to begin centrifugation.
!Platform_manufacture_protocol = 10. Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm.
!Platform_manufacture_protocol = 11. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.
!Platform_manufacture_protocol = 12. For each subsequent preparation of these plates for a print run, add water to the wells instead of sodium phosphate buffer. The amount of water should be decreased by 0.25 L per print run, as this is the amount drawn up by the pin capillary during each dip.
!Platform_support = glass
!Platform_coating = polysine
!Platform_web_link = http://www.microarray.protocols.html
!Platform_contributor = Jane,Doe
!Platform_contributor = John,A,Smith
!Platform_contributor = Hans,van Elton
!Platform_contributor = John,Smithers Jr
!Platform_contributor = Jie,D,Chen
#ID =
#GB_ACC = GenBank accession number of sequence used to design oligonucleotide probe LINK_PRE:"http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=Nucleotide&term="
#Gene_Desc = Gene description
#Gene_Sym = Gene symbols
#SEQUENCE = Probe sequence information
#SPOT_ID = alternative identifier
!platform_table_begin
ID GB_ACC Gene_Desc Gene_Sym SPOT_ID SEQUENCE
1 U02079 nuclear factor of activated T-cells, cytoplasmic 2 Nfatc2 ACCTGGATGACGCAGCCACTTCAGAAAGCTGGGTTGGGACAGAAAGGTATATAGAGAGAAAATTTTGGAA
2 NM_008154 G-protein coupled receptor 3 Gpr3 CTGTACAATGCTCTCACTTACTACTCAGAGACAACGGTAACTCGGACTTATGTGATGCTGGCCTTGGTGT
3 AK015719 tropomodulin 2 Tmod2 CACCAGGCTCAGTGCCTAGTATCGGCTTCACCTAGTGTGGTTACTCAGGGCACGCAGAGCTACAGAACAC
4 AK003367 mitochondrial ribosomal protein L15 Mrpl15 CAAGAAGTCTAGAAATTCTGTGCAAGCCTATTCCATTCTTTCTGCGGGGACAACCAATTCCGAAAAGAAT
5 BC003333 RIKEN cDNA 0610033I05 gene 0610033I05Rik AGAACTGGGTGGCAGATATCCTAGAGTTTTGACCAACGTTCACAGCACACATATTGATCTTATAGGACCT
6 NM_008462 killer cell lectin-like receptor, subfamily A, member 2 Klra2 TGAATTGAAGTTCCTTAAATCCCAACTTCAAAGAAACACATACTGGATTTCACTGACACATCATAAAAGC
7 NM_008029 FMS-like tyrosine kinase 4 Flt4 GAGGTGCTGTGGGATGACCGCCGGGGCATGCGGGTGCCCACTCAACTGTTGCGCGATGCCCTGTACCTGC
8 NM_054088 adiponutrin Adpn GTCTGAGTTCCATTCCAAAGACGAAGTCGTGGATGCCCTGGTGTGTTCCTGCTTCATTCCCCTCTTCTCT
9 NM_009750 nerve growth factor receptor (TNFRSF16) associated protein 1 Ngfrap1 TACAGCTGAGAAATTGTCTACGCATCCTTATGGGGGAGCTGTCTAACCACCACGATCACCATGATGAATT
10 AB045323 DNA segment, Chr 8, ERATO Doi 594, expressed D8Ertd594e GATTCAGACTCGGGAGGAGCATCCCAACCTCTCCTTGAGGATAAAGGCCTGAGCGATTGCCCTGGGGAGC
11 AK005789 dynein, cytoplasmic, light chain 2B Dncl2b TGCAGAAGGCATTCCAATCCGAACAACCCTGGACAACTCCACAACGGTTCAGTATGCGGGTCTTCTCCAC
12 NM_010517 insulin-like growth factor binding protein 4 Igfbp4 GGAGAAGCTGGCGCGCTGCCGCCCCCCCGTGGGTTGCGAGGAGTTGGTGCGGGAGCCAGGCTGCGGTTGT
13 AK010722 RIKEN cDNA 2410075D05 gene 2410075D05Rik GGAGCATCTGGAGTTCCGCTTACCGGAAATAAAGTCTTTACTATCGGTGATTGGAGGGCAGTTCACTAAC
14 AK003755 DNA segment, Chr 4, ERATO Doi 421, expressed D4Ertd421e AGCAAAGAGATCTCCCTCAGTGTGCCCATAGGTGGCGGTGCGAGCTTGCGGTTATTGGCCAGTGACTTGC
15 BC003241 cleavage stimulation factor, 3' pre-RNA, subunit 3 Cstf3 AAATTAGAAGAAAATCCATATGACCTTGATGCTTGGAGCATTCTCATTCGAGAGGCACAGAATCAACCTA
16 AK004937 RIKEN cDNA 1300007O09 gene 1300007O09Rik CAGACACAAACCCTAGGTTGTATTGTAGACCGGAGTTTAAGCAGGCACTACCTGTCTGTCTTTTCTTCAT
17 AK004524 unnamed protein product; hypothetical SOCS domain CGGAGCCCTGCGCGCCCAGAGCCCCCTCCCACCCGCTTCCACCAAGTGCATGGAGCCAACATCCGCATGG
18 NM_025999 RIKEN cDNA 2610110L04 gene 2610110L04Rik TGCATTGATAAATGGAGTGATCGACACAGGAACTGCCCCATTTGTCGCCTACAGATGACTGGAGCAAATG
19 -- CONTROL
20 NM_023120 guanine nucleotide binding protein (G protein), beta polypeptide 1-like Gnb1l ACCGCCTGGTCCCAGATTTGTCCTCCGAGGCACACAGTCGGCTGTGAACACGCTCCATTTCTGCCCACCA
!platform_table_end
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