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{PDOC00640}
{PS00812; GLYCOSYL_HYDROL_F8}
{BEGIN}
******************************************
* Glycosyl hydrolases family 8 signature *
******************************************
The microbial degradation of cellulose and xylans requires several types of
enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91)
(exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces
a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the
basis of sequence similarities, can be classified into families. One of these
families is known as the cellulase family D [3] or as the glycosyl hydrolases
family 8 [4,E1]. The enzymes which are currently known to belong to this
family are listed below.
- Acetobacter xylinum endonuclease cmcAX.
- Bacillus strain KSM-330 acidic endonuclease K (Endo-K).
- Cellulomonas josui endoglucanase 2 (celB).
- Cellulomonas uda endoglucanase.
- Clostridium cellulolyticum endoglucanases C (celcCC).
- Clostridium thermocellum endoglucanases A (celA).
- Erwinia chrysanthemi minor endoglucanase y (celY).
- Bacillus circulans beta-glucanase (EC 3.2.1.73).
- Escherichia coli hypothetical protein yhjM.
The most conserved region in these enzymes is a stretch of about 20 residues
that contains two conserved aspartate. The first asparatate is thought [5] to
act as the nucleophile in the catalytic mechanism. We have used this region as
a signature pattern.
-Consensus pattern: A-[ST]-D-[AG]-D-x(2)-[IM]-A-x-[SA]-[LIVM]-[LIVMG]-x-A-
x(3)-[FW]
[The first D is an active site residue]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Expert(s) to contact by email:
Henrissat B.; bernie@afmb.cnrs-mrs.fr
-Last update: November 1997 / Text revised.
[ 1] Beguin P.
"Molecular biology of cellulose degradation."
Annu. Rev. Microbiol. 44:219-248(1990).
PubMed=2252383; DOI=10.1146/annurev.mi.44.100190.001251
[ 2] Gilkes N.R., Henrissat B., Kilburn D.G., Miller R.C. Jr., Warren R.A.J.
"Domains in microbial beta-1, 4-glycanases: sequence conservation,
function, and enzyme families."
Microbiol. Rev. 55:303-315(1991).
PubMed=1886523
[ 3] Henrissat B., Claeyssens M., Tomme P., Lemesle L., Mornon J.-P.
"Cellulase families revealed by hydrophobic cluster analysis."
Gene 81:83-95(1989).
PubMed=2806912
[ 4] Henrissat B.
"A classification of glycosyl hydrolases based on amino acid sequence
similarities."
Biochem. J. 280:309-316(1991).
PubMed=1747104
[ 5] Alzari P.M., Souchon H., Dominguez R.
"The crystal structure of endoglucanase CelA, a family 8 glycosyl
hydrolase from Clostridium thermocellum."
Structure 4:265-275(1996).
PubMed=8805535
[E1] http://www.expasy.org/cgi-bin/lists?glycosid.txt
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{END}
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