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<HTML><HEAD><TITLE>RJR REBASE Enz 2540 - I-CreI</TITLE>
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<TD bgColor=#ffffff width="70%"><B><FONT color=#c90000
size=6>I-CreI<BR></FONT></B><FONT color=#dd7700 size=2>Homing
endonuclease </FONT></TD>
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<TD bgColor=#ffffff><FONT color=#000080 size=1><B>NOTE:</B> Homing
endonucleases do not really have recognition sequences in the way
that restriction enzymes do. The recognition sequence listed is one
site that is known to be recognized and cleaved. In general, single
base changes merely change the efficiency of cleavage and the
precise boundary of required bases is not known.
</FONT></TD></TR></TBODY></TABLE></CENTER>
<CENTER><BR><FONT color=#000000 size=2><B>Recognition Sequence:</B></FONT>
<PRE><FONT color=#0000dd size=2><B> C T G G G T T C A A A A C G T C G T G A^G A C A G T T T G G 0 <BR> G A C C C A A G T T T T G C A G^C A C T C T G T C A A A C C 0 </B></FONT></PRE></CENTER>
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<TD bgColor=#ffffff><FONT color=#000000 size=2><B>REBASE
enzyme #:</B> 2540<BR><B>Prototype:
</B>I-CreI<BR><B>Source: </B><A
href="http://rebase.neb.com/cgi-bin/sourget?502">J.-D.
Rochaix</A><BR><B>Microorganism: </B><A
href="http://rebase.neb.com/cgi-bin/reb_get.pl?searchcat=m&searchkey=Chlamydomonas+reinhardtii"><I>Chlamydomonas</I>
<I>reinhardtii</I> </A><BR><B>Growth Temperature: </B>26
<BR>Enzyme gene <B>cloned.</B><BR>Enzyme gene
<B>sequenced.</B><BR><B>Crystal data
present.</B><BR><B>Comment: </B><FONT size=1>Insertion
site CGT^CGT</FONT><BR><FONT size=1><B>Entered:</B> Jan
1 1979 ... <B>Modified:</B> Sep 9 1999
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<TD bgColor=#ffffff><FONT size=1><B># sites
on</B><BR><I>Adeno2: </I>0<BR><I>Lambda: </I>0<BR><I>pBR322:
</I>0<BR><I>PhiX174: </I>0<BR><I>SV40: </I>0
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<TD><A href="http://rebase.neb.com/cgi-bin/erefget?2540"><FONT
color=#802080 size=2><B>Related References...</B></FONT><BR><FONT
color=#802080 size=1>(sorted by date)</FONT></A> <A
href="http://rebase.neb.com/cgi-bin/erefget2?2540"><FONT
color=#802080 size=1>(sorted by authors)</FONT></A></TD>
<TD><FONT size=2><B>NOT commercially available</B></FONT></TD>
<TD><FONT size=2><A
href="http://rebase.neb.com/cgi-bin/seqget?I-CreI"><FONT
color=#5577cc><B>Sequence Data...</B></FONT></A> </FONT></TD>
<TD><A href="http://rebase.neb.com/cgi-bin/cryget?I-CreI"><FONT
color=#0088a8 size=2><B>Crystal
Data...</B></FONT></A></TD></TR></TBODY></TABLE></CENTER><!-- ___ Note: enz_code: R --><!-- ___ Note: enz_type: -8 --><!-- ___ Note: sub_type: -->
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<TD bgColor=#ffffff width="40%"><FONT color=#008000
size=1>07/13/2000</FONT><BR><A
href="mailto:roberts@neb.com"><FONT color=#008000 size=1>Dr.
Richard J. Roberts</FONT></A><BR><A
href="mailto:macelis@neb.com"><FONT color=#008000 size=1>and
Dana Macelis</FONT></A><BR></TD>
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