ID   1A02_HUMAN     STANDARD;      PRT;   365 AA.
AC   P01892; P06338; P30514; P30444; P30445; P30446; Q29680; Q29899;
AC   Q95352; Q29837; Q95380;
DT   21-JUL-1986 (Rel. 01, Created)
DT   13-AUG-1987 (Rel. 05, Last sequence update)
DT   15-FEB-2000 (Rel. 39, Last annotation update)
DE   HLA CLASS I HISTOCOMPATIBILITY ANTIGEN, A-2 ALPHA CHAIN PRECURSOR.
GN   HLA-A OR HLAA.
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia;
OC   Eutheria; Primates; Catarrhini; Hominidae; Homo.
RN   [1]
RP   SEQUENCE FROM N.A. (A*0201).
RX   MEDLINE; 85132727.
RA   Koller B.H., Orr H.T.;
RT   "Cloning and complete sequence of an HLA-A2 gene: analysis of two
RT   HLA-A alleles at the nucleotide level.";
RL   J. Immunol. 134:2727-2733(1985).
RN   [2]
RP   SEQUENCE FROM N.A. (A*0201).
RX   MEDLINE; 89122144.
RA   Cianetti L., Testa U., Scotto L., la Valle R., Simeone A.,
RA   Boccoli G., Giannella G., Peschle C., Boncinelli E.;
RT   "Three new class I HLA alleles: structure of mRNAs and alternative
RT   mechanisms of processing.";
RL   Immunogenetics 29:80-91(1989).
RN   [3]
RP   SEQUENCE FROM N.A. (A*0201).
RX   MEDLINE; 90207291.
RA   Ennis P.D., Zemmour J., Salter R.D., Parham P.;
RT   "Rapid cloning of HLA-A,B cDNA by using the polymerase chain
RT   reaction: frequency and nature of errors produced in amplification.";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:2833-2837(1990).
RN   [4]
RP   SEQUENCE FROM N.A. (A*0201/A*0211/A*0212).
RX   MEDLINE; 92269955.
RA   Belich M.P., Madrigal J.A., Hildebrand W.H., Zemmour J.,
RA   Williams R.C., Luz R., Petzl-Erler M.L., Parham P.;
RT   "Unusual HLA-B alleles in two tribes of Brazilian Indians.";
RL   Nature 357:326-329(1992).
RN   [5]
RP   SEQUENCE OF 39-365 FROM N.A. (A*0201).
RX   MEDLINE; 85230571.
RA   Krangel M.S.;
RT   "Unusual RNA splicing generates a secreted form of HLA-A2 in a
RT   mutagenized B lymphoblastoid cell line.";
RL   EMBO J. 4:1205-1210(1985).
RN   [6]
RP   SEQUENCE OF 25-295 (A*0201).
RX   MEDLINE; 80056745.
RA   Orr H.T., Lopez de Castro J.A., Parham P., Ploegh H.L.,
RA   Strominger J.L.;
RT   "Comparison of amino acid sequences of two human histocompatibility
RT   antigens, HLA-A2 and HLA-B7: location of putative alloantigenic
RT   sites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:4395-4399(1979).
RN   [7]
RP   REVISIONS (A*0201).
RX   MEDLINE; 82247941.
RA   Lopez de Castro J.A., Strominger J.L., Strong D.M., Orr H.T.;
RT   "Structure of crossreactive human histocompatibility antigens HLA-A28
RT   and HLA-A2: possible implications for the generation of HLA
RT   polymorphism.";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:3813-3817(1982).
RN   [8]
RP   SEQUENCE OF 26-298 FROM N.A. (A*0202/A*0203).
RX   MEDLINE; 87306734.
RA   Mattson D.H., Handy D.E., Bradley D.A., Coligan J.E., Cowan E.P.,
RA   Biddison W.E.;
RT   "DNA sequences of the genes that encode the CTL-defined HLA-A2
RT   variants M7 and DK1.";
RL   Immunogenetics 26:190-192(1987).
RN   [9]
RP   SEQUENCE FROM N.A. (A*0203/A*0205).
RX   MEDLINE; 87252273.
RA   Holmes N., Ennis P., Wan A.M., Denney D.W., Parham P.;
RT   "Multiple genetic mechanisms have contributed to the generation of
RT   the HLA-A2/A28 family of class I MHC molecules.";
RL   J. Immunol. 139:936-941(1987).
RN   [10]
RP   SEQUENCE FROM N.A. (A*0203/A*0205).
RA   Domena J.D.;
RL   Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
RN   [11]
RP   SEQUENCE OF 9-365 FROM N.A. (A*0204).
RX   MEDLINE; 92039809.
RA   Castano A.R., Lopez de Castro J.A.;
RT   "Structure of the HLA-A*0204 antigen, found in South American
RT   Indians. Spatial clustering of HLA-A2 subtype polymorphism.";
RL   Immunogenetics 34:281-285(1991).
RN   [12]
RP   SEQUENCE OF 9-365 FROM N.A. (A*0204).
RX   MEDLINE; 92269956.
RA   Watkins D.I., McAdam S.N., Liu X., Stang C.R., Milford E.L.,
RA   Levine C.G., Garber T.L., Dogon A.L., Lord C.I., Ghim S.H.,
RA   Troup G.M., Hughes A.L., Letvin N.L.;
RT   "New recombinant HLA-B alleles in a tribe of South American
RT   Amerindians indicate rapid evolution of MHC class I loci.";
RL   Nature 357:329-333(1992).
RN   [13]
RP   SEQUENCE FROM N.A. (A*0206).
RX   MEDLINE; 89235215.
RA   Parham P., Lawlor D.A., Lomen C.E., Ennis P.D.;
RT   "Diversity and diversification of HLA-A,B,C alleles.";
RL   J. Immunol. 142:3937-3950(1989).
RN   [14]
RP   PARTIAL SEQUENCE (A*0206).
RX   MEDLINE; 86305811.
RA   Ezquerra A., Domenech N., van der Poel J., Strominger J.L., Vega M.A.,
RA   Lopez de Castro J.A.;
RT   "Molecular analysis of an HLA-A2 functional variant CLA defined by
RT   cytolytic T lymphocytes.";
RL   J. Immunol. 137:1642-1649(1986).
RN   [15]
RP   PARTIAL SEQUENCE (A*0207).
RX   MEDLINE; 88113844.
RA   Domenech N., Ezquerra A., Castano R., Lopez de Castro J.A.;
RT   "Structural analysis of HLA-A2.4 functional variant KNE. Implications
RT   for the mapping of HLA-A2-specific T-cell epitopes.";
RL   Immunogenetics 27:196-202(1988).
RN   [16]
RP   PARTIAL SEQUENCE (A*0208).
RX   MEDLINE; 88314183.
RA   Domenech N., Castano R., Goulmy E., Lopez de Castro J.A.;
RT   "Molecular analysis of HLA-A2.4 functional variant KLO: close
RT   structural and evolutionary relatedness to the HLA-A2.2 subtype.";
RL   Immunogenetics 28:143-152(1988).
RN   [17]
RP   PARTIAL SEQUENCE (A*0209).
RX   MEDLINE; 88186100.
RA   Castano R., Ezquerra A., Domenech N., Lopez de Castro J.A.;
RT   "An HLA-A2 population variant with structural polymorphism in the
RT   alpha 3 region.";
RL   Immunogenetics 27:345-355(1988).
RN   [18]
RP   SEQUENCE FROM N.A. (A*0210).
RX   MEDLINE; 89122133.
RA   Epstein H., Kennedy L., Holmes N.;
RT   "An Oriental HLA-A2 subtype is closely related to a subset of
RT   Caucasoid HLA-A2 alleles.";
RL   Immunogenetics 29:112-116(1989).
RN   [19]
RP   SEQUENCE OF 9-365 FROM N.A. (A*0211).
RX   MEDLINE; 92218010.
RA   Castano A.R., Lopez de Castro J.A.;
RT   "Structure of the HLA-A*0211 (A2.5) subtype: further evidence for
RT   selection-driven diversification of HLA-A2 antigens.";
RL   Immunogenetics 35:344-346(1992).
RN   [20]
RP   SEQUENCE FROM N.A. (A*0213).
RX   MEDLINE; 94222455.
RA   Barber D.F., Fernandez J.M., Lopez de Castro J.A.;
RT   "Primary structure of a new HLA-A2 subtype: HLA-A*0213.";
RL   Immunogenetics 39:378-378(1994).
RN   [21]
RP   SEQUENCE FROM N.A. (A*0216).
RX   MEDLINE; 95278976.
RA   Barouch D., Krausa P., Bodmer J., Browning M.J., McMichael A.J.;
RT   "Identification of a novel HLA-A2 subtype, HLA-A*0216.";
RL   Immunogenetics 41:388-388(1995).
RN   [22]
RP   SEQUENCE FROM N.A. (A*0217).
RC   TISSUE=BLOOD;
RX   MEDLINE; 95381236.
RA   Selvakumar A., Granja C.B., Salazar M., Alosco S.M., Yunis E.J.,
RA   Dupont B.;
RT   "A novel subtype of A2 (A*0217) isolated from the South American
RT   Indian B-cell line AMALA.";
RL   Tissue Antigens 45:343-347(1995).
RN   [23]
RP   SEQUENCE FROM N.A. (A*0218).
RC   TISSUE=BLOOD;
RA   Kashiwase K., Tokunaga K., Ishikawa Y., Oohashi H., Hashimoto M.,
RA   Akaza T., Tadokoro K., Juji T.;
RT   "A new A2 sequence HLA-A2K from Japanese.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [24]
RP   SEQUENCE FROM N.A. (A*0220).
RC   TISSUE=BLOOD;
RX   MEDLINE; 97161038.
RA   Fleischhauer K., Zino E., Mazzi B., Severini G.M., Benazzi E.,
RA   Bordignon C.;
RT   "HLA-A*02 subtype distribution in Caucasians from northern Italy:
RT   identification of A*0220.";
RL   Tissue Antigens 48:673-679(1996).
RN   [25]
RP   SEQUENCE FROM N.A. (A*0221).
RC   TISSUE=BLOOD;
RA   Szmania S., Baxter-Lowe L.A.;
RT   "Nucleotide sequence of a novel HLA-A2 gene.";
RL   Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN   [26]
RP   X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF A*0201.
RX   MEDLINE; 88014204.
RA   Bjorkman P.J., Saper M.A., Samraoui B., Bennett W.S.,
RA   Strominger J.L., Wiley D.C.;
RT   "Structure of the human class I histocompatibility antigen, HLA-A2.";
RL   Nature 329:506-512(1987).
RN   [27]
RP   X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF A*0201.
RX   MEDLINE; 91245570.
RA   Saper M.A., Bjorkman P.J., Wiley D.C.;
RT   "Refined structure of the human histocompatibility antigen HLA-A2 at
RT   2.6-A resolution.";
RL   J. Mol. Biol. 219:277-319(1991).
CC   -!- FUNCTION: INVOLVED IN THE PRESENTATION OF FOREIGN ANTIGENS TO
CC       THE IMMUNE SYSTEM.
CC   -!- SUBUNIT: DIMER OF ALPHA CHAIN AND A BETA CHAIN (BETA-2-
CC       MICROGLOBULIN).
CC   -!- POLYMORPHISM: THE FOLLOWING ALLELES OF A-2 ARE KNOWN: A*0201,
CC       A*0202, A*0203, A*0204, A*0205, A*0206 (A2.4A), A*0207, A*0208,
CC       A*0209, A*0210, A*0211 (A2.5), A*0212, A*0213 (A*02SLU), A*0216,
CC       A*0217, A*0218 (A2K), A*0219, A*0220 AND A*0221. THE SEQUENCE
CC       SHOWN IS THAT OF A*0201.
CC   -!- CAUTION: REF.6 AND REF.7 SEQUENCES DIFFER FROM THAT SHOWN
CC       EXTENSIVELY.
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DR   EMBL; K02883; AAA98727.1; -.
DR   EMBL; M84379; AAA59606.1; -.
DR   EMBL; X02457; CAA26297.1; -.
DR   EMBL; M19670; AAA03683.1; -.
DR   EMBL; M17690; AAB02120.1; -.
DR   EMBL; U03863; AAA03604.1; -.
DR   EMBL; M86404; -; NOT_ANNOTATED_CDS.
DR   EMBL; X57954; CAA41022.1; ALT_SEQ.
DR   EMBL; U03862; AAA03603.1; -.
DR   EMBL; M24042; AAA59653.1; -.
DR   EMBL; Z23071; CAA80612.1; -.
DR   EMBL; M84377; AAA59603.1; -.
DR   EMBL; X60764; -; NOT_ANNOTATED_CDS.
DR   EMBL; M84378; AAA59604.1; -.
DR   EMBL; Z27120; CAA81644.1; -.
DR   EMBL; Z46633; CAA86602.1; -.
DR   EMBL; U18930; AAA87076.1; -.
DR   EMBL; D83515; BAA11935.1; -.
DR   EMBL; X96724; CAA65501.1; -.
DR   EMBL; U56825; AAB17465.1; -.
DR   PIR; A02188; HLHUA2.
DR   PIR; A02191; HLHU2A.
DR   PIR; E35997; E35997.
DR   PIR; S19020; S19020.
DR   PIR; S23593; S23593.
DR   PDB; 1HLA; 15-JUL-90.
DR   PDB; 3HLA; 15-APR-90.
DR   PDB; 1HHG; 31-OCT-93.
DR   PDB; 1HHH; 31-OCT-93.
DR   PDB; 1HHI; 31-OCT-93.
DR   PDB; 1HHJ; 31-OCT-93.
DR   PDB; 1HHK; 31-OCT-93.
DR   PDB; 1AQD; 28-JAN-98.
DR   MIM; 142800; -.
DR   PROSITE; PS00290; IG_MHC; 1.
DR   PFAM; PF00047; ig; 1.
DR   PFAM; PF00129; MHC_I; 1.
KW   MHC I; Transmembrane; Glycoprotein; Signal; Polymorphism;
KW   3D-structure.
FT   SIGNAL        1     24
FT   CHAIN        25    365       HLA CLASS I HISTOCOMPATIBILITY ANTIGEN,
FT                                A-2 ALPHA CHAIN.
FT   DOMAIN       25    114       EXTRACELLULAR ALPHA-1.
FT   DOMAIN      115    206       EXTRACELLULAR ALPHA-2.
FT   DOMAIN      207    298       EXTRACELLULAR ALPHA-3.
FT   DOMAIN      299    308       CONNECTING PEPTIDE.
FT   TRANSMEM    309    332
FT   DOMAIN      333    365       CYTOPLASMIC TAIL.
FT   CARBOHYD    110    110
FT   DISULFID    125    188
FT   DISULFID    227    283
FT   STRAND       27     36
FT   STRAND       45     52
FT   TURN         53     54
FT   STRAND       55     61
FT   TURN         62     63
FT   STRAND       70     71
FT   HELIX        74     76
FT   TURN         77     78
FT   HELIX        81    108
FT   TURN        109    110
FT   TURN        113    114
FT   STRAND      118    127
FT   TURN        129    130
FT   STRAND      133    142
FT   TURN        143    144
FT   STRAND      145    150
FT   TURN        152    153
FT   STRAND      157    159
FT   TURN        163    163
FT   HELIX       164    173
FT   TURN        174    175
FT   HELIX       176    185
FT   TURN        186    186
FT   HELIX       187    198
FT   TURN        199    199
FT   HELIX       200    203
FT   TURN        204    204
FT   STRAND      207    207
FT   STRAND      210    219
FT   TURN        220    221
FT   STRAND      222    233
FT   STRAND      238    243
FT   TURN        244    245
FT   STRAND      246    247
FT   HELIX       249    251
FT   STRAND      253    254
FT   STRAND      258    259
FT   STRAND      265    274
FT   TURN        275    276
FT   HELIX       278    280
FT   STRAND      281    286
FT   TURN        288    289
FT   STRAND      294    297
FT   VARIANT      33     33       F -> Y (IN A*0205, A*0206, A*0208, A*0210
FT                                AND A*0221).
FT                                /FTId=VAR_004334.
FT   VARIANT      54     54       D -> N (IN A*0221).
FT                                /FTId=VAR_004335.
FT   VARIANT      67     67       Q -> R (IN A*0202, A*0205, AND A*0208).
FT                                /FTId=VAR_004336.
FT   VARIANT      90     90       K -> N (IN A*0208 AND A*0220).
FT                                /FTId=VAR_004337.
FT   VARIANT      97     98       TH -> ID (IN A*0211).
FT                                /FTId=VAR_004338.
FT   VARIANT     119    119       V -> L (IN A*0202, A*0205, A*0208 AND
FT                                A*0217).
FT                                /FTId=VAR_004339.
FT   VARIANT     121    121       R -> M (IN A*0204 AND A*0217).
FT                                /FTId=VAR_004340.
FT   VARIANT     123    123       Y -> C (IN A*0207 AND A*0218).
FT                                /FTId=VAR_004341.
FT   VARIANT     123    123       Y -> F (IN A*0210 AND A*0217).
FT                                /FTId=VAR_004342.
FT   VARIANT     131    131       W -> G (IN A*0210).
FT                                /FTId=VAR_004343.
FT   VARIANT     162    162       M -> K (IN A*0218).
FT                                /FTId=VAR_004344.
FT   VARIANT     173    173       A -> T (IN A*0203).
FT                                /FTId=VAR_004345.
FT   VARIANT     176    176       V -> E (IN A*0203 AND A*0213).
FT                                /FTId=VAR_004346.
FT   VARIANT     180    180       L -> W (IN A*0202, A*0203, A*0205 AND
FT                                A*0208).
FT                                /FTId=VAR_004347.
FT   VARIANT     180    180       L -> Q (IN A*0212 AND A*0213).
FT                                /FTId=VAR_004348.
FT   VARIANT     187    187       T -> E (IN A*0216).
FT                                /FTId=VAR_004349.
FT   VARIANT     260    260       A -> E (IN A*0209).
FT                                /FTId=VAR_004350.
SQ   SEQUENCE   365 AA;  40922 MW;  B54A97B24B337C08 CRC64;
     MAVMAPRTLV LLLSGALALT QTWAGSHSMR YFFTSVSRPG RGEPRFIAVG YVDDTQFVRF
     DSDAASQRME PRAPWIEQEG PEYWDGETRK VKAHSQTHRV DLGTLRGYYN QSEAGSHTVQ
     RMYGCDVGSD WRFLRGYHQY AYDGKDYIAL KEDLRSWTAA DMAAQTTKHK WEAAHVAEQL
     RAYLEGTCVE WLRRYLENGK ETLQRTDAPK THMTHHAVSD HEATLRCWAL SFYPAEITLT
     WQRDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGQEQR YTCHVQHEGL PKPLTLRWEP
     SSQPTIPIVG IIAGLVLFGA VITGAVVAAV MWRRKSSDRK GGSYSQAASS DSAQGSDVSL
     TACKV
//