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TBLASTN 2.2.26+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: minirefseq_mrna
23 sequences; 67,750 total letters
Query= random_s00
Length=32
***** No hits found *****
Effective search space used: 0
Query= gi|16080617|ref|NP_391444.1| membrane bound lipoprotein [Bacillus
subtilis subsp. subtilis str. 168]
Length=102
Score E
Sequences producing significant alignments: (Bits) Value
gi|145479850|ref|XM_001425911.1| Paramecium tetraurelia hypothe... 34.7 1e-05
gi|72012412|ref|XM_777959.1| PREDICTED: Strongylocentrotus purp... 31.6 1e-04
gi|115975252|ref|XM_001180111.1| PREDICTED: Strongylocentrotus ... 31.6 1e-04
> gi|145479850|ref|XM_001425911.1| Paramecium tetraurelia hypothetical
protein (GSPATT00004923001) partial mRNA
Length=4632
Score = 34.7 bits (78), Expect = 1e-05, Method: Composition-based stats.
Identities = 15/43 (35%), Positives = 26/43 (60%), Gaps = 0/43 (0%)
Frame = +1
Query 31 PDSNIETKEGTYVGLADTHTIEVTVDNEPVSLDITEESTSDLD 73
P + TK+GT +GL HTI + + +SL++ E++ D+D
Sbjct 1744 PKTATGTKKGTIIGLLSIHTILFILTSHALSLEVKEQT*KDID 1872
> gi|72012412|ref|XM_777959.1| PREDICTED: Strongylocentrotus purpuratus
hypothetical LOC577746 (LOC577746), mRNA
Length=1593
Score = 31.6 bits (70), Expect = 1e-04, Method: Composition-based stats.
Identities = 20/59 (34%), Positives = 29/59 (49%), Gaps = 8/59 (14%)
Frame = +1
Query 44 GLADTHTIEVTVDNEPVSLDITEESTSDLDKFNSG--------DKVTITYEKNDEGQLL 94
GL HT+ + V + LD+TEE ++LD+F S K + YE+N Q L
Sbjct 1057 GLVPDHTLILPVGHYQSMLDLTEEVQTELDQFKSALRKYYLSKGKTCVIYERNFRTQHL 1233
> gi|115975252|ref|XM_001180111.1| PREDICTED: Strongylocentrotus
purpuratus hypothetical LOC577746 (LOC577746), mRNA
Length=1593
Score = 31.6 bits (70), Expect = 1e-04, Method: Composition-based stats.
Identities = 20/59 (34%), Positives = 29/59 (49%), Gaps = 8/59 (14%)
Frame = +1
Query 44 GLADTHTIEVTVDNEPVSLDITEESTSDLDKFNSG--------DKVTITYEKNDEGQLL 94
GL HT+ + V + LD+TEE ++LD+F S K + YE+N Q L
Sbjct 1057 GLVPDHTLILPVGHYQSMLDLTEEVQTELDQFKSALRKYYLSKGKTCVIYERNFRTQHL 1233
Lambda K H
0.302 0.125 0.332
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 1205400
Query= gi|11464971:4-101 pleckstrin [Mus musculus]
Length=98
Score E
Sequences producing significant alignments: (Bits) Value
gi|350596019|ref|XM_003360601.2| PREDICTED: Sus scrofa pleckstr... 199 2e-67
gi|301779869|ref|XM_002925302.1| PREDICTED: Ailuropoda melanole... 202 2e-67
gi|296223671|ref|XM_002757683.1| PREDICTED: Callithrix jacchus ... 202 4e-67
gi|338714227|ref|XM_001492113.3| PREDICTED: Equus caballus plec... 202 2e-66
gi|365982352|ref|XM_003667962.1| Naumovozyma dairenensis CBS 42... 19.6 1.7
gi|94721341|ref|NM_001040441.1| Homo sapiens zinc finger and BT... 17.7 6.3
gi|332865370|ref|XM_527768.3| PREDICTED: Pan troglodytes zinc f... 17.3 8.7
gi|332865372|ref|XM_003318468.1| PREDICTED: Pan troglodytes zin... 17.3 8.7
> gi|350596019|ref|XM_003360601.2| PREDICTED: Sus scrofa pleckstrin-like
(LOC100626968), mRNA
Length=772
Score = 199 bits (506), Expect = 2e-67, Method: Compositional matrix adjust.
Identities = 94/98 (96%), Positives = 96/98 (98%), Gaps = 0/98 (0%)
Frame = +2
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGS+FNTWKPMWV+LLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 95 KRIREGYLVKKGSMFNTWKPMWVILLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 274
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERD WVRDIKKAIK
Sbjct 275 KRMFVFKITTTKQQDHFFQAAFLEERDGWVRDIKKAIK 388
Score = 32.7 bits (73), Expect = 4e-05, Method: Compositional matrix adjust.
Identities = 21/71 (30%), Positives = 33/71 (46%), Gaps = 4/71 (6%)
Frame = +2
Query 30 IEFYKKKSDNSPKGMIPLKGSTLTS-PCQDFGKRMFVLK---ITTTKQQDHFFQAAFLEE 85
+ +Y P G I L+G +TS GK F+ + T + +F QAA +E
Sbjct 542 LHYYDPAGGEDPLGAIHLRGCVVTSVESNTDGKNGFLWERAXXITADEVHYFLQAANPKE 721
Query 86 RDAWVRDIKKA 96
R W++ I+ A
Sbjct 722 RTEWIKAIQVA 754
> gi|301779869|ref|XM_002925302.1| PREDICTED: Ailuropoda melanoleuca
pleckstrin-like (LOC100466932), mRNA
Length=1144
Score = 202 bits (515), Expect = 2e-67, Method: Compositional matrix adjust.
Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Frame = +3
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVK+GSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 78 KRIREGYLVKRGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 257
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 258 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 371
Score = 45.1 bits (105), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Frame = +3
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS----PC 56
I++G L+K+G WK +L ED + +Y P G I L+G +TS P
Sbjct 804 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNPD 983
Query 57 QDFGKRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
+ + +I T + +F QAA +ER W++ I+ A
Sbjct 984 VRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA 1103
> gi|296223671|ref|XM_002757683.1| PREDICTED: Callithrix jacchus
pleckstrin (PLEK), mRNA
Length=1183
Score = 202 bits (515), Expect = 4e-67, Method: Compositional matrix adjust.
Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Frame = +2
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGS+FNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 161 KRIREGYLVKKGSMFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 340
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 341 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 454
Score = 45.1 bits (105), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Frame = +2
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
I++G L+K+G WK +L ED + +Y P G I L+G +TS +
Sbjct 866 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNSD 1045
Query 61 KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
R + +I T + +F QAA +ER W++ I+ A
Sbjct 1046 GRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA 1165
> gi|338714227|ref|XM_001492113.3| PREDICTED: Equus caballus pleckstrin-like
(LOC100051039), mRNA
Length=1390
Score = 202 bits (515), Expect = 2e-66, Method: Compositional matrix adjust.
Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Frame = +2
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVK+GSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 173 KRIREGYLVKRGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 352
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 353 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 466
Score = 46.6 bits (109), Expect = 1e-09, Method: Compositional matrix adjust.
Identities = 31/100 (31%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Frame = +2
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS----PC 56
I++G L+K+G WK VL ED + +Y P G I L+G +TS P
Sbjct 899 IKQGCLLKQGHRRKNWKVRKFVLREDPAYVHYYDPAGGEEPLGAIHLRGCVVTSVEGNPD 1078
Query 57 QDFGKRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
+ + +I T + +F QAA +ER W++ I+ A
Sbjct 1079 GKKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA 1198
> gi|365982352|ref|XM_003667962.1| Naumovozyma dairenensis CBS
421 hypothetical protein (NDAI0A06120), mRNA
Length=4932
Score = 19.6 bits (39), Expect = 1.7, Method: Compositional matrix adjust.
Identities = 16/52 (31%), Positives = 23/52 (44%), Gaps = 9/52 (17%)
Frame = +1
Query 12 GSVFNTWKPMWVVLL---------EDGIEFYKKKSDNSPKGMIPLKGSTLTS 54
GS F TW +++ +L E E K D + KG++ L S TS
Sbjct 3181 GSCFPTWDLIFIEVLNPFLKEKLWEADNEEISKFVDLTLKGLVDLYPSHFTS 3336
Lambda K H
0.321 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 1119300
Database: minirefseq_mrna
Posted date: Jun 9, 2012 2:05 PM
Number of letters in database: 67,750
Number of sequences in database: 23
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40
|
