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ID U87107 standard; DNA; SYN; 8840 BP.
XX
AC U87107;
XX
SV U87107.1
XX
DT 15-OCT-1997 (Rel. 52, Created)
DT 15-OCT-1997 (Rel. 52, Last updated, Version 4)
XX
DE Cloning vector pAL-F insertion sequence IS1 galactokinase (galK),
DE aminoglycoside 3'-phosphotransferase (kn), beta-galactosidase (lacZ), small
DE ribosomal protein and beta-lactamase (Ap) genes, complete cds.
XX
KW .
XX
OS Cloning vector pAL-F
OC artificial sequence; vectors.
XX
RN [1]
RP 1-8840
RA Ahmed A., Podemski L.;
RT "Use of ordered deletions in genome sequencing";
RL Gene 197:367-373(1997).
XX
RN [2]
RP 1-8840
RA Ahmed A.;
RT ;
RL Submitted (27-JAN-1997) to the EMBL/GenBank/DDBJ databases.
RL Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9,
RL Canada
XX
DR REMTREMBL; AAC53713; AAC53713.
DR REMTREMBL; AAC53714; AAC53714.
DR REMTREMBL; AAC53715; AAC53715.
DR REMTREMBL; AAC53716; AAC53716.
DR REMTREMBL; AAC53717; AAC53717.
XX
FH Key Location/Qualifiers
FH
FT source 1..8840
FT /db_xref="taxon:56954"
FT /organism="Cloning vector pAL-F"
FT /insertion_seq="IS1"
FT /specific_host="Escherichia coli"
FT CDS complement(933..2081)
FT /codon_start=1
FT /db_xref="REMTREMBL:AAC53713"
FT /transl_table=11
FT /gene="galK"
FT /product="galactokinase"
FT /protein_id="AAC53713.1"
FT /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVLP
FT CAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKHLQ
FT LRNNSFGGVDMVDHGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQEAEN
FT QFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTLVGSE
FT YNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTVEAASA
FT LEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGGGFGGCI
FT VALIPEELVPAVQQRVAEQYEAKTGIKETFYVCKPSQGAGQC"
FT CDS 2875..3669
FT /codon_start=1
FT /db_xref="REMTREMBL:AAC53714"
FT /transl_table=11
FT /gene="kn"
FT /function="kanamycin resistance"
FT /product="aminoglycoside 3'-phosphotransferase"
FT /protein_id="AAC53714.1"
FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
FT CDS 3978..4442
FT /codon_start=1
FT /db_xref="REMTREMBL:AAC53715"
FT /note="alpha fragment"
FT /transl_table=11
FT /gene="lacZ"
FT /product="beta-galactosidase"
FT /protein_id="AAC53715.1"
FT /translation="MTMITNSCRRPRARGTVDGSLIKFKSPARRARVDGTTSLALAVVL
FT QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRDKLALYALMQF
FT LCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWIDPAEFYADQ"
FT CDS complement(4555..4929)
FT /codon_start=1
FT /db_xref="REMTREMBL:AAC53716"
FT /transl_table=11
FT /function="streptomycin sensitivity"
FT /product="small ribosomal protein"
FT /protein_id="AAC53716.1"
FT /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKKP
FT NSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDC
FT SGVKDRKQARSKYGVKRPKA"
FT CDS complement(7087..7947)
FT /codon_start=1
FT /db_xref="REMTREMBL:AAC53717"
FT /transl_table=11
FT /gene="Ap"
FT /function="ampicillin resistance"
FT /product="beta-lactamase"
FT /protein_id="AAC53717.1"
FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
FT LIKHW"
XX
SQ Sequence 8840 BP; 2068 A; 2288 C; 2319 G; 2165 T; 0 other;
caattactgc aatgccctcg taattaagtg aatttacaat atcgtcctgt tcggagggaa 60
gaacgcggga tgttcattct tcatcacttt taattgatgt atatgctctc ttttctgacg 120
ttagtctccg acggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgct 180
caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg 240
ttctgcgggt tctgttcttc gttgacatga ggttgccccg tattcagtgt cgctgatttg 300
tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt 360
cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagatgat 420
aatcattatc actttacggg tcctttccgg tgatccgaca ggttacgggg cggcgacctc 480
gcgggttttc gctatttatg aaaattttcc ggtttaaggc gtttccgttc ttcttcgtca 540
taacttaatg tttttattta aaataccctc tgaaaagaaa ggaaacgaca ggtgctgaaa 600
gcgaggcttt ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg 660
gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac cattcagaac 720
tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga ggtgctttat 780
gactctgccg ccgtcataaa atggtatgcc gaaagggatg ctgaaattga gaacgaaaag 840
ctgcgccggg aggttgaaga actgcggcag gccagcgagg cagatcaaca gtcggtacgg 900
ctgaccatcg ggtgccagtg cgggagtttc gttcagcact gtcctgctcc ttgtgatggt 960
ttacaaacgt aaaaagtctc tttaatacct gtttttgctt catattgttc agcgacacgt 1020
tgctgtacgg caggcaccag ctcttccggg atcagcgcga cgatacagcc gccaaatccg 1080
ccgccggtca tgcgtacgcc acctttgtcg ccaatcacag ctttgacgat ttctaccaga 1140
gtgtcaattt gcggcacggt gatttcgaaa tcatcgcgca tagaggcatg agactccgcc 1200
atcaactcgc ccatacgttt caggtcgcct tgctccagcg cgctggcagc ttcaacggtg 1260
cgggcgtttt cagtcagtat atgacgcacg cgttttgcca cgatcgggtc cagttcatgc 1320
gcaacagcgt tgaactcttc aatggtgaca tcacgcaggg ctggctgctg gaagaaacgc 1380
gcaccggttt cgcactgttc acgacgggtg ttgtattcgc tgccaaccag ggtacgtttg 1440
aagttactgt tgatgatgac gacagccaca cctttgggca tggaaactgc tttggtcccc 1500
agtgagcggc aatcgatcag caaggcatga tctttcttgc cgagcgcgga aattagctga 1560
tccatgatcc cgcagttaca gcctacaaac tggttttctg cttcctgacc gttaagcgcg 1620
atttgtgcgc cgtccagcgg cagatgataa agctgctgca atacggttcc gaccgcgact 1680
tccagtgaag cggaagaact taacccggca ccctgcggca cattgccgtg atcaaccatg 1740
tccacgccgc cgaagctgtt gttacgcagt tgcagatgtt tcaccacgcc acgaacgtag 1800
ttagcccatt gatagttttc atgtgcgaca atgggcgcat cgagggaaaa ctcgtcgagc 1860
tgattttcat aatcggctgc catcacgcga actttacggt catcgcgtgg tgcacaactg 1920
atcacggttt gataatcaat cgcgcagggc agaacgaaac cgtcgttgta gtcggtgtgt 1980
tcaccaatca aattcacgcg gccaggcgcc tgaatggtgt gagtggcagg gtagccaaat 2040
gcgttggcaa acagagattg tgttttttct ttcagactca tttcttacac tccggattcg 2100
cgacggccta cagcaacctg ggcgatgtat ggcatcaggt tattcggaat gccttgcgga 2160
tcttcgccca tatcgcccga cggatgcgcg ccaaccgggt tgaagtagcg cacgagggca 2220
atgctccagt ccggctgggc tttttgcaga tcggtgagga tctgttccac catcagcttg 2280
cttttgccgt aagggctttg cggtgtgccg gtcgggaagc tataatgcgg tagtttatca 2340
cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc 2400
atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg 2460
ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg cgtgctgcta 2520
gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg gaacacgtag 2580
aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg 2640
acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga 2700
tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc 2760
tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc 2820
tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 2880
gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 2940
gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 3000
gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaagac 3060
gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 3120
gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 3180
ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 3240
ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 3300
cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 3360
caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc cgacggcgag 3420
gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 3480
ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 3540
ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 3600
ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 3660
ttcttctgag cgggactctg gggttcgcga tgataagctg tcaaacatga gaattacaac 3720
ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc 3780
tagaaatatt ttatctgatt cgattcatta atgcagctgg cacgacaggt ttcccgactg 3840
gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 3900
ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 3960
tcacacagga aacagctatg accatgatta cgaattcctg caggcggccg cgagctcgag 4020
gtaccgtcga cggatcctta attaaattta aatcaccggc gcgccgagct cgagtcgacg 4080
gtaccacaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 4140
ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 4200
aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcgata 4260
agctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg 4320
accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg 4380
cgatcatggc gaccacaccc gtcctgtgga tcgatccggc agaattttac gctgaccaat 4440
gacgcgacga cgtggcatgg aaatactccg ttgttaattc aggattgtcc aaaactctac 4500
gagtttagtt tgacatttaa gttaaaacgt ttagccttac ttaacggaga accattaagc 4560
cttaggacgc ttcacgccat acttggaacg agcctgctta cggtctttaa cgccggagca 4620
gtcaagcgca ccacgtacgg tgtggtaacg aacacccggg aggtctttaa cacgaccgcc 4680
acggatcagg atcacggagt gctcctgcag gttgtgacct tcaccaccga tgtaggaagt 4740
cacttcgaaa ccgttagtca gacgaacacg gcatacttta cgcagcgcgg agttcggttt 4800
tttaggagtg gtagtatata cacgagtaca tacgccacgt ttttgcgggc atgcttccag 4860
cgcaggcacg ttgcttttcg caactttgcg agcacgtggt ttgcgtacca gctggttaac 4920
tgttgccatt aaatagctcc tggttttagc ttttgcttcg taaacacgta ataaaacgtc 4980
ctcacacaat atgaggacgc cgaattttag ggcgatgccg aaaaggtgtc aagaaatata 5040
caacgatccc gccatcatca ccaggccatc tggctggggt gcttaaccgt aagtctgacg 5100
aaatcagtat agtcaatgag aatgatgtcg ttcgaaattt gaccagtcaa acccaaacca 5160
acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct 5220
cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc 5280
ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa 5340
cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg 5400
gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc 5460
ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga 5520
agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt 5580
gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga 5640
gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg 5700
aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc 5760
agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct 5820
gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg 5880
atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 5940
cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 6000
gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc 6060
atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 6120
aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 6180
ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 6240
agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6300
ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6360
caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6420
gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6480
cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6540
tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6600
gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6660
cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6720
tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6780
tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6840
caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6900
aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6960
cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 7020
ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 7080
tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 7140
atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 7200
tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 7260
aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7320
catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7380
gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7440
ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7500
aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7560
atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7620
cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7680
gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 7740
agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7800
gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7860
caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7920
ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7980
tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 8040
aggggttccg cgcacatttc cccgaaaagt gcggtaatga ctccaactta ttgatagtgt 8100
tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac 8160
agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt 8220
cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga ttcatacagc 8280
ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct cataagacgc 8340
cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt ccggagactg 8400
tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacatagcc ccactgttcg 8460
tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt taccgactgc 8520
ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata atgcgggctg 8580
ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggtt 8640
gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag cagagatagc 8700
gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg 8760
gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc 8820
agtaagttgg cagcatcacc 8840
//
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