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LOCUS pBAD30 4923 bp DNA circular
SOURCE
ORGANISM
COMMENT This file is best viewed with a free Vector NTI Viewer:
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|GenBank
COMMENT VNTREPLTYPE|Phagemid
COMMENT VNTEXTCHREPL|Bacteria
FEATURES Location/Qualifiers
rep_origin 1..843
/vntifkey="33"
/label=P15A_ORI
CDS complement(1082..1960)
/vntifkey="4"
/label=araC
/note="araC regulator of the arabinose BAD promoter"
misc_feature 1989..2006
/vntifkey="21"
/label=operator\O2
/note="operator O2"
promoter complement(2111..2139)
/vntifkey="30"
/label=araC\promoter
/note="araC promoter"
misc_feature 2147..2168
/vntifkey="21"
/label=operator\O1
/note="operator O1"
misc_feature 2190..2203
/vntifkey="21"
/label=CAP\site
/note="CAP site"
misc_feature 2199..2237
/vntifkey="21"
/label=operator\I2\and\I1
/note="operator I2 + I1"
promoter 2236..2263
/vntifkey="30"
/label=arabinose\BAD\promoter
/note="arabinose pBAD promoter"
misc_feature 2286..2348
/vntifkey="21"
/label=MCS
/note="multiple cloning site"
terminator 2349..2774
/vntifkey="43"
/label=rrnB\T1\T2
promoter 2829..2835
/vntifkey="30"
/label=P(BLA)
/note="bla P3 promoter"
CDS 2867..3730
/vntifkey="4"
/label=AP(R)
rep_origin 3766..4224
/vntifkey="33"
/label=M13\ORI
/note="M13 origin"
BASE COUNT 1240 a 1274 c 1181 g 1228 t
ORIGIN
1 gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct
61 tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata
121 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa
181 atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa
241 gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
301 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
361 ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
421 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca
481 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta
541 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca
601 gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac
661 tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt
721 ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca
781 agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata
841 tttgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
901 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
961 gatctgctca tgtttgacag cttatcatcg atgcataatg tgcctgtcaa atggacgaag
1021 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca
1081 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
1141 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
1201 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
1261 ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa
1321 gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat
1381 tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg
1441 gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat
1501 ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa
1561 acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa
1621 atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact
1681 ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga
1741 acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct
1801 gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa
1861 aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg
1921 agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat
1981 tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact
2041 ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg
2101 ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc
2161 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
2221 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt
2281 tttgggctag cgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat
2341 gcaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag
2401 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac
2461 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc
2521 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
2581 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
2641 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg
2701 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg
2761 tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
2821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
2881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
2941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
3001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
3061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
3121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
3181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
3241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
3301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
3361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
3421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
3481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
3541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
3601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
3661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
3721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttac gcgccctgta
3781 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
3841 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
3901 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
3961 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
4021 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
4081 aaacttgaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
4141 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
4201 acaaaatatt aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc
4261 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
4321 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
4381 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
4441 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
4501 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
4561 ttaccagtca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg
4621 taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt
4681 cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
4741 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
4801 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acagacggca
4861 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
4921 atg
//
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