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BLASTX 2.2.26+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: NCBI Protein Reference Sequences
11,879,989 sequences; 4,140,237,112 total letters
Query= gi|356995852:1-490 Mus musculus POU domain, class 5, transcription
factor 1 (Pou5f1), transcript variant 1, mRNA
Length=490
Score E
Sequences producing significant alignments: (Bits) Value
ref|NP_038661.2| POU domain, class 5, transcription factor 1 iso... 192 4e-57
ref|NP_001009178.1| POU class 5 homeobox 1 [Rattus norvegicus] 179 3e-52
ref|XP_003505911.1| PREDICTED: POU domain, class 5, transcriptio... 147 7e-40
ref|XP_001490158.1| PREDICTED: POU domain, class 5, transcriptio... 141 8e-38
ref|XP_001135162.1| PREDICTED: POU domain, class 5, transcriptio... 138 1e-36
ref|NP_002692.2| POU domain, class 5, transcription factor 1 iso... 138 2e-36
ref|XP_002809144.1| PREDICTED: POU domain, class 5, transcriptio... 137 2e-36
ref|XP_528230.1| PREDICTED: putative POU domain, class 5, transc... 133 1e-34
ref|XP_002746363.1| PREDICTED: POU domain, class 5, transcriptio... 133 1e-34
ref|NP_001108427.1| POU domain, class 5, transcription factor 1 ... 133 1e-34
ref|NP_001106531.1| POU domain, class 5, transcription factor 1 ... 133 1e-34
ref|XP_002752527.1| PREDICTED: POU domain, class 5, transcriptio... 132 2e-34
ref|NP_001166912.1| POU domain, class 5, transcription factor 1 ... 131 6e-34
ref|NP_777005.1| POU domain, class 5, transcription factor 1 [Bo... 131 6e-34
ref|NP_001153014.1| putative POU domain, class 5, transcription ... 128 6e-33
ref|NP_001093427.1| POU domain, class 5, transcription factor 1 ... 127 2e-32
ref|XP_538830.1| PREDICTED: POU domain, class 5, transcription f... 119 1e-29
ref|XP_003422494.1| PREDICTED: POU domain, class 5, transcriptio... 118 4e-29
ref|XP_003474038.1| PREDICTED: POU domain, class 5, transcriptio... 117 7e-29
ref|XP_003271964.1| PREDICTED: LOW QUALITY PROTEIN: POU domain, ... 111 7e-27
>ref|NP_038661.2| POU domain, class 5, transcription factor 1 isoform 1 [Mus musculus]
Length=352
Score = 192 bits (487), Expect = 4e-57
Identities = 140/140 (100%), Positives = 140/140 (100%), Gaps = 0/140 (0%)
Frame = +3
Query 69 MAGHLAsdfafspppgggdgsagLEPGWVDPRTWLSFQgppggpgigpgSEVLGISPCPP 248
MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPGIGPGSEVLGISPCPP
Sbjct 1 MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPGIGPGSEVLGISPCPP 60
Query 249 AYEFCGGMAYCgpqvglglvpqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE 428
AYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE
Sbjct 61 AYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE 120
Query 429 KVEPTPEESQDMKALQKELE 488
KVEPTPEESQDMKALQKELE
Sbjct 121 KVEPTPEESQDMKALQKELE 140
>ref|NP_001009178.1| POU class 5 homeobox 1 [Rattus norvegicus]
Length=352
Score = 179 bits (454), Expect = 3e-52
Identities = 133/140 (95%), Positives = 135/140 (96%), Gaps = 0/140 (0%)
Frame = +3
Query 69 MAGHLAsdfafspppgggdgsagLEPGWVDPRTWLSFQgppggpgigpgSEVLGISPCPP 248
MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPP GPGIGPGSEVLGISPCPP
Sbjct 1 MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPSGPGIGPGSEVLGISPCPP 60
Query 249 AYEFCGGMAYCgpqvglglvpqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE 428
AYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG SS PC RP+AVKLE
Sbjct 61 AYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGASSGPCTARPSAVKLE 120
Query 429 KVEPTPEESQDMKALQKELE 488
KVEP+PEESQDMKALQKELE
Sbjct 121 KVEPSPEESQDMKALQKELE 140
>ref|XP_003505911.1| PREDICTED: POU domain, class 5, transcription factor 1-like [Cricetulus
griseus]
Length=358
Score = 147 bits (370), Expect = 7e-40
Identities = 120/145 (83%), Positives = 128/145 (88%), Gaps = 5/145 (3%)
Frame = +3
Query 69 MAGHLAsdfafspppgggdgsagLEPGWVDPRTWLSFQgppggpgigpg----SEVLGIS 236
MAGHLASDFAFSPPPGGGDGS GLEPGWVDPRTWLSFQGPPGGPGIGPG SEVLGIS
Sbjct 1 MAGHLASDFAFSPPPGGGDGSGGLEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVLGIS 60
Query 237 PCPPAYEFCGGMAYCgpqvglglvpqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNA 416
PCPP YEFCGG+AYCGPQVGLGLVP VG+ET QPEGQ+GA VE++SE +S PC RP
Sbjct 61 PCPPPYEFCGGVAYCGPQVGLGLVPPVGLETSQPEGQSGAGVENDSEESSPGPCTARPIV 120
Query 417 -VKLEKVEPTPEESQDMKALQKELE 488
VKLEKVEP+PEESQD+KALQKELE
Sbjct 121 PVKLEKVEPSPEESQDVKALQKELE 145
>ref|XP_001490158.1| PREDICTED: POU domain, class 5, transcription factor 1-like [Equus
caballus]
Length=360
Score = 141 bits (356), Expect = 8e-38
Identities = 93/122 (76%), Positives = 101/122 (83%), Gaps = 6/122 (5%)
Frame = +3
Query 141 EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv 308
EPGWVDPRTWLSFQGPP G GIGPG +EV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct 26 EPGWVDPRTWLSFQGPPSGSGIGPGVGPGAEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV 85
Query 309 pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE 482
PQ +ET QPEG+AGARVESNSEG S EPCA P AVK+ EK+E PEESQD+KALQK+
Sbjct 86 PQGSLETSQPEGEAGARVESNSEGASPEPCAAPPGAVKVDKEKLEQNPEESQDIKALQKD 145
Query 483 LE 488
LE
Sbjct 146 LE 147
>ref|XP_001135162.1| PREDICTED: POU domain, class 5, transcription factor 1 isoform
2 [Pan troglodytes]
Length=359
Score = 138 bits (348), Expect = 1e-36
Identities = 95/121 (79%), Positives = 101/121 (83%), Gaps = 5/121 (4%)
Frame = +3
Query 141 EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv 308
EPGWVDPRTWLSFQGPPGGPGIGPG SEV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct 26 EPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV 85
Query 309 pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLE-KVEPTPEESQDMKALQKEL 485
PQ G+ET QPEG+AG VESNS+G S EPC P AVKLE K+E PEESQD+KALQKEL
Sbjct 86 PQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKKLEQNPEESQDIKALQKEL 145
Query 486 E 488
E
Sbjct 146 E 146
>ref|NP_002692.2| POU domain, class 5, transcription factor 1 isoform 1 [Homo sapiens]
ref|NP_001238970.1| POU domain, class 5, transcription factor 1 [Pan troglodytes]
Length=360
Score = 138 bits (347), Expect = 2e-36
Identities = 95/122 (78%), Positives = 101/122 (83%), Gaps = 6/122 (5%)
Frame = +3
Query 141 EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv 308
EPGWVDPRTWLSFQGPPGGPGIGPG SEV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct 26 EPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV 85
Query 309 pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE 482
PQ G+ET QPEG+AG VESNS+G S EPC P AVKL EK+E PEESQD+KALQKE
Sbjct 86 PQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKE 145
Query 483 LE 488
LE
Sbjct 146 LE 147
>ref|XP_002809144.1| PREDICTED: POU domain, class 5, transcription factor 1-like [Pongo
abelii]
Length=360
Score = 137 bits (346), Expect = 2e-36
Identities = 94/122 (77%), Positives = 100/122 (82%), Gaps = 6/122 (5%)
Frame = +3
Query 141 EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv 308
EPGWVDPRTWLSFQGPPGGPGIGPG SEV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct 26 EPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV 85
Query 309 pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE 482
PQ +ET QPEG+AG VESNS+G S EPC P AVKL EK+E PEESQD+KALQKE
Sbjct 86 PQGSLETSQPEGEAGVGVESNSDGASPEPCTVPPGAVKLEKEKLEQNPEESQDIKALQKE 145
Query 483 LE 488
LE
Sbjct 146 LE 147
>ref|XP_528230.1| PREDICTED: putative POU domain, class 5, transcription factor
1B [Pan troglodytes]
Length=359
Score = 133 bits (334), Expect = 1e-34
Identities = 93/122 (76%), Positives = 98/122 (80%), Gaps = 6/122 (5%)
Frame = +3
Query 141 EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv 308
EPGWVDP TWL FQGPPGGPGIGPG SEV GI PCPP YEFCGGMAYCGPQVG GLV
Sbjct 26 EPGWVDPLTWLRFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGAGLV 85
Query 309 pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE 482
PQ G+ET QPEG+AG VESNS+G S EPC P AVKL EK+E PEESQD+KALQKE
Sbjct 86 PQGGLETSQPEGEAGVGVESNSDGASPEPCTVPPGAVKLEKEKLEQNPEESQDIKALQKE 145
Query 483 LE 488
LE
Sbjct 146 LE 147
>ref|XP_002746363.1| PREDICTED: POU domain, class 5, transcription factor 1-like [Callithrix
jacchus]
Length=360
Score = 133 bits (334), Expect = 1e-34
Identities = 94/122 (77%), Positives = 100/122 (82%), Gaps = 6/122 (5%)
Frame = +3
Query 141 EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv 308
E GWVDPRTWLSFQGPPGGPGIGPG +EV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct 26 ETGWVDPRTWLSFQGPPGGPGIGPGVGPGAEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV 85
Query 309 pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE 482
PQ G+ET QPEG+AGA VESNSEG S EPC P AVKL EK+E EESQD+KALQKE
Sbjct 86 PQGGLETSQPEGEAGAGVESNSEGASPEPCTIPPGAVKLEKEKLEQNTEESQDIKALQKE 145
Query 483 LE 488
LE
Sbjct 146 LE 147
>ref|NP_001108427.1| POU domain, class 5, transcription factor 1 [Macaca mulatta]
Length=360
Score = 133 bits (334), Expect = 1e-34
Identities = 94/122 (77%), Positives = 100/122 (82%), Gaps = 6/122 (5%)
Frame = +3
Query 141 EPGWVDPRTWLSFQgppggpgigpg----SEVLGISPCPPAYEFCGGMAYCgpqvglglv 308
E GWVDPRTWLSFQGPPGGPGIGPG SEV GI PCPP YEFCGGMAYCGPQVG+GLV
Sbjct 26 ETGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLV 85
Query 309 pqvgvETLQPEGQAGARVESNSEGTSSEPCADRPNAVKL--EKVEPTPEESQDMKALQKE 482
PQ G+ET QPEG+AGA VESNS+G S EPC AVKL EK+E PEESQD+KALQKE
Sbjct 86 PQGGLETSQPEGEAGAGVESNSDGASPEPCTVPTGAVKLEKEKLEQNPEESQDIKALQKE 145
Query 483 LE 488
LE
Sbjct 146 LE 147
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 104017620564
Database: NCBI Protein Reference Sequences
Posted date: Mar 18, 2012 8:41 PM
Number of letters in database: 4,140,237,112
Number of sequences in database: 11,879,989
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 12
Window for multiple hits: 40
|
