1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
|
BLASTP 2.2.26+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: NCBI Protein Reference Sequences
11,879,989 sequences; 4,140,237,112 total letters
Query= gi|11464971:4-101 pleckstrin [Mus musculus]
Length=98
Score E
Sequences producing significant alignments: (Bits) Value
ref|NP_062422.1| pleckstrin [Mus musculus] 205 1e-63
ref|XP_003502426.1| PREDICTED: pleckstrin-like [Cricetulus griseus] 205 2e-63
ref|NP_002655.2| pleckstrin [Homo sapiens] 204 7e-63
ref|XP_002811995.1| PREDICTED: pleckstrin-like [Pongo abelii] 204 7e-63
ref|XP_003360649.2| PREDICTED: pleckstrin-like [Sus scrofa] 199 1e-62
ref|XP_001094492.1| PREDICTED: pleckstrin [Macaca mulatta] 203 1e-62
ref|NP_001020921.1| pleckstrin [Rattus norvegicus] 202 2e-62
ref|XP_002925348.1| PREDICTED: pleckstrin-like [Ailuropoda melan... 202 2e-62
ref|XP_001492163.1| PREDICTED: pleckstrin-like [Equus caballus] 202 2e-62
ref|XP_003262529.1| PREDICTED: pleckstrin [Nomascus leucogenys] 202 2e-62
ref|XP_002757729.1| PREDICTED: pleckstrin [Callithrix jacchus] 201 3e-62
ref|XP_515524.2| PREDICTED: pleckstrin [Pan troglodytes] 201 4e-62
ref|XP_003125127.2| PREDICTED: pleckstrin [Sus scrofa] 200 2e-61
ref|NP_001003363.1| pleckstrin [Canis lupus familiaris] 199 3e-61
ref|NP_001179425.1| pleckstrin [Bos taurus] 197 2e-60
ref|XP_001382141.2| PREDICTED: pleckstrin [Monodelphis domestica] 194 2e-59
ref|NP_990194.1| pleckstrin [Gallus gallus] 190 1e-57
ref|XP_003203732.1| PREDICTED: pleckstrin-like [Meleagris gallop... 190 1e-57
ref|XP_003468982.1| PREDICTED: pleckstrin-like [Cavia porcellus] 188 5e-57
ref|XP_002199907.1| PREDICTED: pleckstrin [Taeniopygia guttata] 187 1e-56
>ref|NP_062422.1| pleckstrin [Mus musculus]
Length=350
Score = 205 bits (522), Expect = 1e-63, Method: Compositional matrix adjust.
Identities = 98/98 (100%), Positives = 98/98 (100%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 43.5 bits (101), Expect = 0.002, Method: Compositional matrix adjust.
Identities = 29/100 (29%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS--PCQD 58
I++G L+K+G WK +L ED + +Y P G + L+G +TS D
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAVHLRGCVVTSVESSHD 305
Query 59 FGK--RMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
K + +I T + ++ QAA +ER W++ I+ A
Sbjct 306 VKKSDEENLFEIITADEVHYYLQAATSKERTEWIKAIQVA 345
>ref|XP_003502426.1| PREDICTED: pleckstrin-like [Cricetulus griseus]
Length=350
Score = 205 bits (521), Expect = 2e-63, Method: Compositional matrix adjust.
Identities = 98/98 (100%), Positives = 98/98 (100%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 43.9 bits (102), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 50/100 (50%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDF- 59
I++G L+K+G WK +L ED + +Y P G I L+G +TS +
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNHD 305
Query 60 GKRM---FVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
GK+ + +I T + ++ QAA +ER W++ I+ A
Sbjct 306 GKKSDDENLFEIITADEVHYYLQAAAPKERTEWIKAIQVA 345
>ref|NP_002655.2| pleckstrin [Homo sapiens]
Length=350
Score = 204 bits (518), Expect = 7e-63, Method: Compositional matrix adjust.
Identities = 97/98 (99%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 47.4 bits (111), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 31/100 (31%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
I++G L+K+G WK +L ED + +Y P G I L+G +TS +
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSN 305
Query 61 KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
R + +I T + +F QAA +ER W+R I+ A
Sbjct 306 GRKSEEENLFEIITADEVHYFLQAATPKERTEWIRAIQMA 345
>ref|XP_002811995.1| PREDICTED: pleckstrin-like [Pongo abelii]
Length=350
Score = 204 bits (518), Expect = 7e-63, Method: Compositional matrix adjust.
Identities = 97/98 (99%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 45.4 bits (106), Expect = 4e-04, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
I++G L+K+G WK +L ED + +Y P G I L+G +TS +
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSN 305
Query 61 KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
R + +I T + +F QAA +ER W++ I+ A
Sbjct 306 GRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA 345
>ref|XP_003360649.2| PREDICTED: pleckstrin-like [Sus scrofa]
Length=228
Score = 199 bits (506), Expect = 1e-62, Method: Compositional matrix adjust.
Identities = 94/98 (96%), Positives = 96/98 (98%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGS+FNTWKPMWV+LLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSMFNTWKPMWVILLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERD WVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDGWVRDIKKAIK 101
>ref|XP_001094492.1| PREDICTED: pleckstrin [Macaca mulatta]
Length=350
Score = 203 bits (516), Expect = 1e-62, Method: Compositional matrix adjust.
Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVK+GSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKRGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 45.4 bits (106), Expect = 4e-04, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
I++G L+K+G WK +L ED + +Y P G I L+G +TS +
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSN 305
Query 61 KRMF----VLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
R + +I T + +F QAA +ER W++ I+ A
Sbjct 306 GRKSEEENLFEIITADEVHYFLQAASPKERTEWIKAIQVA 345
>ref|NP_001020921.1| pleckstrin [Rattus norvegicus]
Length=350
Score = 202 bits (515), Expect = 2e-62, Method: Compositional matrix adjust.
Identities = 96/98 (98%), Positives = 98/98 (100%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDN+PKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNNPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFVLKITTTKQQDHFFQAA+LEERDAWVRDIKKAIK
Sbjct 64 KRMFVLKITTTKQQDHFFQAAYLEERDAWVRDIKKAIK 101
Score = 44.3 bits (103), Expect = 8e-04, Method: Compositional matrix adjust.
Identities = 29/100 (29%), Positives = 47/100 (47%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS----PC 56
I++G L+K+G WK +L ED + +Y P G I L+G +TS P
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNPD 305
Query 57 QDFGKRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
+ +I T + ++ QAA +ER W++ I+ A
Sbjct 306 GKKSDEENLFEIITADEVHYYMQAATAKERTEWIKAIQVA 345
>ref|XP_002925348.1| PREDICTED: pleckstrin-like [Ailuropoda melanoleuca]
Length=350
Score = 202 bits (515), Expect = 2e-62, Method: Compositional matrix adjust.
Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVK+GSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKRGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 45.1 bits (105), Expect = 5e-04, Method: Compositional matrix adjust.
Identities = 30/100 (30%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS----PC 56
I++G L+K+G WK +L ED + +Y P G I L+G +TS P
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGGEDPLGAIHLRGCVVTSVESNPD 305
Query 57 QDFGKRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
+ + +I T + +F QAA +ER W++ I+ A
Sbjct 306 VRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA 345
>ref|XP_001492163.1| PREDICTED: pleckstrin-like [Equus caballus]
Length=350
Score = 202 bits (515), Expect = 2e-62, Method: Compositional matrix adjust.
Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVK+GSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKRGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Score = 46.6 bits (109), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 31/100 (31%), Positives = 48/100 (48%), Gaps = 6/100 (6%)
Query 3 IREGYLVKKGSVFNTWKPMWVVLLEDG--IEFYKKKSDNSPKGMIPLKGSTLTS----PC 56
I++G L+K+G WK VL ED + +Y P G I L+G +TS P
Sbjct 246 IKQGCLLKQGHRRKNWKVRKFVLREDPAYVHYYDPAGGEEPLGAIHLRGCVVTSVEGNPD 305
Query 57 QDFGKRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKA 96
+ + +I T + +F QAA +ER W++ I+ A
Sbjct 306 GKKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQVA 345
>ref|XP_003262529.1| PREDICTED: pleckstrin [Nomascus leucogenys]
Length=350
Score = 202 bits (514), Expect = 2e-62, Method: Compositional matrix adjust.
Identities = 96/98 (98%), Positives = 97/98 (99%), Gaps = 0/98 (0%)
Query 1 KRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 60
KRIREGYLVKKGS+FNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG
Sbjct 4 KRIREGYLVKKGSMFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFG 63
Query 61 KRMFVLKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 98
KRMFV KITTTKQQDHFFQAAFLEERDAWVRDIKKAIK
Sbjct 64 KRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIK 101
Lambda K H
0.321 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 103672613319
Database: NCBI Protein Reference Sequences
Posted date: Mar 18, 2012 8:41 PM
Number of letters in database: 4,140,237,112
Number of sequences in database: 11,879,989
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40
|
