File: test_Align_codonalign.py

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# Copyright (C) 2013 by Zheng Ruan (zruan1991@gmail.com)
# This code is part of the Biopython distribution and governed by its
# license. Please see the LICENSE file that should have been included
# as part of this package.

"""Unit tests for CodonAligner and Bio.Align.analysis."""

import unittest

try:
    import numpy as np
except ImportError:
    from Bio import MissingPythonDependencyError

    raise MissingPythonDependencyError(
        "Install numpy if you want to use Bio.Align."
    ) from None


from Bio import Align
from Bio import SeqIO
from Bio.Align import Alignment
from Bio.Align import CodonAligner
from Bio.Align.analysis import calculate_dn_ds
from Bio.Align.analysis import calculate_dn_ds_matrix
from Bio.Align.analysis import mktest
from Bio.Data import CodonTable
from Bio.Seq import Seq
from Bio.SeqRecord import SeqRecord


class TestBasic(unittest.TestCase):
    def test_aligner(self):
        aligner = CodonAligner()
        self.assertEqual(
            str(aligner),
            """\
Codon aligner with parameters
  wildcard: 'X'
  match_score: 1.0
  mismatch_score: 0.0
  frameshift_minus_two_score: -3.0
  frameshift_minus_one_score: -3.0
  frameshift_plus_one_score: -3.0
  frameshift_plus_two_score: -3.0
""",
        )
        aligner.wildcard = "Y"
        aligner.match_score = 2.0
        aligner.mismatch_score = -1.0
        aligner.frameshift_score = -5.0
        aligner.frameshift_two_score = -2.0
        aligner.frameshift_minus_score = -4.0
        self.assertEqual(
            str(aligner),
            """\
Codon aligner with parameters
  wildcard: 'Y'
  match_score: 2.0
  mismatch_score: -1.0
  frameshift_minus_two_score: -4.0
  frameshift_minus_one_score: -4.0
  frameshift_plus_one_score: -5.0
  frameshift_plus_two_score: -2.0
""",
        )
        self.assertEqual(aligner.wildcard, "Y")
        self.assertAlmostEqual(aligner.match_score, 2.0)
        self.assertAlmostEqual(aligner.mismatch_score, -1.0)
        self.assertAlmostEqual(aligner.frameshift_minus_two_score, -4.0)
        self.assertAlmostEqual(aligner.frameshift_minus_one_score, -4.0)
        self.assertAlmostEqual(aligner.frameshift_plus_one_score, -5.0)
        self.assertAlmostEqual(aligner.frameshift_plus_two_score, -2.0)

    def test_alignments(self):
        aligner = CodonAligner()
        aligner.frameshift_score = -1.0
        dna = SeqRecord(Seq("TTTAAAAAAAAATTT"), id="dna")
        pro = SeqRecord(Seq("FKKKF"), id="pro")
        alignments = aligner.align(pro, dna)
        self.assertEqual(len(alignments), 1)
        self.assertAlmostEqual(alignments.score, 5.0)
        alignment = alignments[0]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  F    5
dna               0 TTTAAAAAAAAATTT 15
""",
        )
        dna = SeqRecord(Seq("TTTAAAAAAAATTT"), id="dna")
        score = aligner.score(pro, dna)
        self.assertAlmostEqual(score, 4.0)
        alignments = aligner.align(pro, dna)
        self.assertEqual(len(alignments), 2)
        self.assertAlmostEqual(alignments.score, 4.0)
        alignment = alignments[0]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K   3
dna               0 TTTAAAAAA 9

pro               3 K  F    5
dna               8 AAATTT 14
""",
        )
        alignment = alignments[1]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K   2
dna               0 TTTAAA 6

pro               2 K  K  F    5
dna               5 AAAAAATTT 14
""",
        )
        dna = SeqRecord(Seq("TTTAAAAAAATTT"), id="dna")
        score = aligner.score(pro, dna)
        self.assertAlmostEqual(score, 4.0)
        alignments = aligner.align(pro, dna)
        self.assertEqual(len(alignments), 2)
        self.assertAlmostEqual(alignments.score, 4.0)
        alignment = alignments[0]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K   3
dna               0 TTTAAAAAA 9

pro               3 K  F    5
dna               7 AAATTT 13
""",
        )
        alignment = alignments[1]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K   2
dna               0 TTTAAA 6

pro               2 K  K  F    5
dna               4 AAAAAATTT 13
""",
        )
        dna = SeqRecord(Seq("TTTAAAAAATTT"), id="dna")
        score = aligner.score(pro, dna)
        self.assertAlmostEqual(score, 3.0)
        alignments = aligner.align(pro, dna)
        self.assertEqual(len(alignments), 2)
        self.assertAlmostEqual(alignments.score, 3.0)
        alignment = alignments[0]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K   2
dna               0 TTTAAA 6

pro               2 K   3
dna               5 AAA 8

pro               3 K  F    5
dna               6 AAATTT 12
""",
        )
        alignment = alignments[1]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K   2
dna               0 TTTAAA 6

pro               2 K   3
dna               4 AAA 7

pro               3 K  F    5
dna               6 AAATTT 12
""",
        )
        dna = SeqRecord(Seq("TTTAAAAATTT"), id="dna")
        score = aligner.score(pro, dna)
        self.assertAlmostEqual(score, 3.0)
        alignments = aligner.align(pro, dna)
        self.assertEqual(len(alignments), 1)
        self.assertAlmostEqual(alignments.score, 3.0)
        alignment = alignments[0]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K   2
dna               0 TTTAAA 6

pro               2 K   3
dna               4 AAA 7

pro               3 K  F    5
dna               5 AAATTT 11
""",
        )
        dna = SeqRecord(Seq("TTTAAAAAAAAAATTT"), id="dna")
        alignments = aligner.align(pro, dna)
        self.assertEqual(len(alignments), 6)
        self.assertAlmostEqual(alignments.score, 4.0)
        alignment = alignments[0]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  F    5
dna               0 TTTAAAAAAAAAATT 15
""",
        )
        alignment = alignments[1]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  F    5
dna               1 TTAAAAAAAAAATTT 16
""",
        )
        alignment = alignments[2]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  -K  K  K  F    5
dna               0 TTTAAAAAAAAAATTT 16
""",
        )
        alignment = alignments[3]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  -K  K  F    5
dna               0 TTTAAAAAAAAAATTT 16
""",
        )
        alignment = alignments[4]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  -K  F    5
dna               0 TTTAAAAAAAAAATTT 16
""",
        )
        alignment = alignments[5]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  -F    5
dna               0 TTTAAAAAAAAAATTT 16
""",
        )
        dna = SeqRecord(Seq("TTTAAAAAAAAAAATTT"), id="dna")
        alignments = aligner.align(pro, dna)
        self.assertEqual(len(alignments), 6)
        self.assertAlmostEqual(alignments.score, 4.0)
        alignment = alignments[0]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  F    5
dna               0 TTTAAAAAAAAAAAT 15
""",
        )
        alignment = alignments[1]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  F    5
dna               2 TAAAAAAAAAAATTT 17
""",
        )
        alignment = alignments[2]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  --K  K  K  F    5
dna               0 TTTAAAAAAAAAAATTT 17
""",
        )
        alignment = alignments[3]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  --K  K  F    5
dna               0 TTTAAAAAAAAAAATTT 17
""",
        )
        alignment = alignments[4]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  --K  F    5
dna               0 TTTAAAAAAAAAAATTT 17
""",
        )
        alignment = alignments[5]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  --F    5
dna               0 TTTAAAAAAAAAAATTT 17
""",
        )
        dna = SeqRecord(Seq("TTTAAAAAAAAAAAATTT"), id="dna")
        alignments = aligner.align(pro, dna)
        self.assertEqual(len(alignments), 2)
        self.assertAlmostEqual(alignments.score, 4.0)
        alignment = alignments[0]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  F    5
dna               0 TTTAAAAAAAAAAAA 15
""",
        )
        alignment = alignments[1]
        self.assertEqual(
            str(alignment),
            """\
pro               0 F  K  K  K  F    5
dna               3 AAAAAAAAAAAATTT 18
""",
        )


class TestBuildAndIO(unittest.TestCase):
    def test1(self):
        aligner = CodonAligner()
        nucleotide_records = SeqIO.parse("codonalign/nucl1.fa", "fasta")
        protein_alignment = Align.read("codonalign/pro1.aln", "clustal")
        self.assertEqual(len(protein_alignment.sequences), 3)
        codon_alignments = []
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[0]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 183], [0, 549]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig697         0 R  G  D  Q  R  S  N  F  Q  L  S  P  S  T  M  Q  I  S  T  G  
isotig697         0 AGAGGCGATCAACGCAGCAACTTCCAGCTGTCTCCCTCCACCATGCAGATCTCCACAGGG

isotig697        20 L  L  C  L  L  L  V  A  T  G  F  T  S  Q  V  L  A  H  P  G  
isotig697        60 CTTCTGTGCcTGCTGCTTGTGGCCACTGGCTTCACTTCCCAGGTGCTGGCTCACCCAGGC

isotig697        40 S  I  P  S  T  Y  C  F  V  M  T  S  K  K  I  P  K  S  L  L  
isotig697       120 TCTATCCCATCTACCTaCTGCTTTGTTATGACCAGTAAGAaGATCCCCAAATCACTACTG

isotig697        60 K  S  Y  K  R  I  S  N  S  R  C  T  L  K  A  I  L  F  K  T  
isotig697       180 AaGAGCTACAAAaGAATCTCCAACAGCAGATGCACCcTGAAAGCCATACTCTTCAAGACC

isotig697        80 K  S  G  K  E  I  C  A  D  P  K  K  K  W  V  Q  D  A  T  K  
isotig697       240 AAGTCGGGCAAAGAGATCTGTGCTGACCCCAAGAAGAAGTGGGTCcAGGATGCCACAAAG

isotig697       100 H  L  D  Q  I  L  Q  T  P  K  P  T  I  P  S  F  E  T  H  P  
isotig697       300 CACCTGGACCAAATCCTTCAAACTCCAAAACCGACAATCCCCTCTTTTGAGACTCACCCA

isotig697       120 E  T  K  K  C  F  I  H  S  P  F  L  R  R  A  P  R  S  T  Q  
isotig697       360 GAGACTAAGAAATGCTTCATTCATTCTCCATTCCTAAGACGTGCTCCAAGGTCAACTCAG

isotig697       140 H  H  S  P  R  T  W  L  H  L  V  M  D  R  T  E  S  H  Y  V  
isotig697       420 CACCATTCCCCAAGGACTTGGCTTCATTTAGTTATGGATAGAACTGAAAGTCATTATGTT

isotig697       160 Q  N  K  P  D  L  K  R  L  C  N  F  L  N  M  Q  N  L  K  R  
isotig697       480 CAGAATAAGCCAGACTTGAAGAGGTTGTGTAATTTCTTGAATATGCAAAATCTTAAAAGG

isotig697       180 G  A  C   183
isotig697       540 GGGGCATGC 549
""",
        )
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[1]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 65], [0, 195]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  K  V  S  A  A  L  L  C  L  L  L  I  A  A  T  F  I  P  Q  
ENSG00000         0 ATGAAAGTCTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCCAA

ENSG00000        20 G  L  A  Q  P  D  A  I  N  A  P  V  T  C  C  Y  N  F  T  N  
ENSG00000        60 GGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTATAACTTCACCAAT

ENSG00000        40 R  K  I  S  V  Q  R  L  A  S  Y  R  R  I  T  S  S  K  C  P  
ENSG00000       120 AGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAGAATCACCAGCAGCAAGTGTCCC

ENSG00000        60 K  E  A  V  M    65
ENSG00000       180 AAAGAAGCTGTGATG 195
""",
        )
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[2]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 99], [9, 306]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  K  V  S  A  A  L  L  C  L  L  L  I  A  A  T  F  I  P  Q  
ENSG00000         9 ATGAAAGTCTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCCAA

ENSG00000        20 G  L  A  Q  P  D  A  I  N  A  P  V  T  C  C  Y  N  F  T  N  
ENSG00000        69 GGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTATAACTTCACCAAT

ENSG00000        40 R  K  I  S  V  Q  R  L  A  S  Y  R  R  I  T  S  S  K  C  P  
ENSG00000       129 AGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAGAATCACCAGCAGCAAGTGTCCC

ENSG00000        60 K  E  A  V  I  F  K  T  I  V  A  K  E  I  C  A  D  P  K  Q  
ENSG00000       189 AAAGAAGCTGTGATCTTCAAGACCATTGTGGCCAAGGAGATCTGTGCTGACCCCAAGCAG

ENSG00000        80 K  W  V  Q  D  S  M  D  H  L  D  K  Q  T  Q  T  P  K  T  
ENSG00000       249 AAGTGGGTTCAGGATTCCATGGACCACCTGGACAAGCAAACCCAAACTCCGAAGACT

ENSG00000        99
ENSG00000       306
""",
        )
        alignment = protein_alignment.mapall(codon_alignments)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
                np.array([[0, 42, 126, 126, 231, 333, 549],
                          [0,  0,  84,  90, 195, 195, 195],
                          [9,  9,  93,  99, 204, 306, 306]])
                # fmt: on
            )
        )
        self.assertEqual(
            format(alignment, "clustal"),
            """\
isotig69710                         AGAGGCGATCAACGCAGCAACTTCCAGCTGTCTCCCTCCACCATGCAGAT
ENSG00000108691:ENST0000058090      ------------------------------------------ATGAAAGT
ENSG00000108691:ENST0000022583      ------------------------------------------ATGAAAGT

isotig69710                         CTCCACAGGGCTTCTGTGCcTGCTGCTTGTGGCCACTGGCTTCACTTCCC
ENSG00000108691:ENST0000058090      CTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCC
ENSG00000108691:ENST0000022583      CTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCC

isotig69710                         AGGTGCTGGCTCACCCAGGCTCTATC------CCATCTACCTaCTGCTTT
ENSG00000108691:ENST0000058090      AAGGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTAT
ENSG00000108691:ENST0000022583      AAGGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTAT

isotig69710                         GTTATGACCAGTAAGAaGATCCCCAAATCACTACTGAaGAGCTACAAAaG
ENSG00000108691:ENST0000058090      AACTTCACCAATAGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAG
ENSG00000108691:ENST0000022583      AACTTCACCAATAGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAG

isotig69710                         AATCTCCAACAGCAGATGCACCcTGAAAGCCATACTCTTCAAGACCAAGT
ENSG00000108691:ENST0000058090      AATCACCAGCAGCAAGTGTCCCAAAGAAGCTGTGATG-------------
ENSG00000108691:ENST0000022583      AATCACCAGCAGCAAGTGTCCCAAAGAAGCTGTGATCTTCAAGACCATTG

isotig69710                         CGGGCAAAGAGATCTGTGCTGACCCCAAGAAGAAGTGGGTCcAGGATGCC
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      TGGCCAAGGAGATCTGTGCTGACCCCAAGCAGAAGTGGGTTCAGGATTCC

isotig69710                         ACAAAGCACCTGGACCAAATCCTTCAAACTCCAAAACCGACAATCCCCTC
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      ATGGACCACCTGGACAAGCAAACCCAAACTCCGAAGACT-----------

isotig69710                         TTTTGAGACTCACCCAGAGACTAAGAAATGCTTCATTCATTCTCCATTCC
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      --------------------------------------------------

isotig69710                         TAAGACGTGCTCCAAGGTCAACTCAGCACCATTCCCCAAGGACTTGGCTT
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      --------------------------------------------------

isotig69710                         CATTTAGTTATGGATAGAACTGAAAGTCATTATGTTCAGAATAAGCCAGA
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      --------------------------------------------------

isotig69710                         CTTGAAGAGGTTGTGTAATTTCTTGAATATGCAAAATCTTAAAAGGGGGG
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      --------------------------------------------------

isotig69710                         CATGC
ENSG00000108691:ENST0000058090      -----
ENSG00000108691:ENST0000022583      -----


""",
        )

    def test2(self):
        aligner = CodonAligner()
        nucleotide_records = SeqIO.parse("codonalign/nucl2.fa", "fasta")
        protein_alignment = Align.read("codonalign/pro2.aln", "clustal")
        self.assertEqual(len(protein_alignment.sequences), 3)
        codon_alignments = []
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[0]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1094], [0, 3282]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig351         0 E  R  Q  G  R  W  C  V  P  G  E  A  V  E  A  R  V  S  R  S  
isotig351         0 GAAAGGCAGGGTCGctGGTGCGTGCCCGGCGAGGCTGTGGAGGCCcgTGTGTCTAGAAGC

isotig351        20 C  V  R  E  M  A  E  P  G  R  R  R  G  P  R  S  R  G  G  G  
isotig351        60 TGTGTGAGAGAGATGGCGGAACCCGGGAGGAGACGGGGTCCGAGGTCCCGCGGTGGCGGC

isotig351        40 A  G  R  G  A  R  R  A  R  V  A  R  G  R  R  P  R  A  P  Q  
isotig351       120 GCCGGCCGAGGCgCTCGAAGAGCCCGGGTCGCCCGTGGCCGGCGTCCTCGCGCCCCGCAG

isotig351        60 S  L  S  R  L  I  P  D  T  V  L  V  D  L  V  S  D  S  D  E  
isotig351       180 TCTCTGTCCCGGCTCATTCCAGACACGGTGCTTGTGGACTTGGTCAGTGACAGCGACGAG

isotig351        80 E  I  L  E  V  V  A  D  P  V  E  A  P  A  A  R  A  P  A  P  
isotig351       240 GAGATCCTGGAAGTCGTCGCGGACCCGGTAGAAGCGCCCGCCGCCCGGGCcCCcGCGCCG

isotig351       100 A  A  H  G  Q  D  S  D  S  D  S  A  G  A  D  E  G  P  A  G  
isotig351       300 GCCGCACATGGGCAGGACAGCGACAGCGACAGTGCAGGGGCGgACGAGGGGCCTGCAGGA

isotig351       120 A  P  Q  T  L  V  R  R  R  R  R  R  L  L  D  P  G  E  A  P  
isotig351       360 GCCCCTCAGACCTTGGTCCGGCGGCGGCGCCGGCGGCTGCTGGATCCCGGCGAGGCACCG

isotig351       140 V  V  P  V  Y  S  G  K  V  Q  S  S  L  N  L  I  P  D  N  S  
isotig351       420 GTGGTTCCTGTGTACTCCGGGAAGGTACAAAGCAGCCTCAACCTCATCCCAGATAATTCA

isotig351       160 S  L  L  K  L  C  P  S  E  P  E  D  E  A  D  V  T  D  C  G  
isotig351       480 TCCCTCTTGAAACTTTGCCCCTCAGAGCCTGAAGATGAGGCAGATGTGACAGATTGTGGC

isotig351       180 S  P  P  P  E  D  A  L  I  P  G  S  P  W  K  K  K  L  R  N  
isotig351       540 AGTCCTCCTCCTGAGGATGCCCTAATTCCAGGTTCTCCCTGGAAGAAGAAGCTGAGGAAT

isotig351       200 K  H  E  K  E  E  M  K  M  E  E  F  P  D  Q  D  I  S  P  L  
isotig351       600 AAGCATGAAAAaGAAGAGATGAAGATGGAAGAGTTtCCGGACCAGGACATCTCTCCTTTG

isotig351       220 P  R  P  S  S  R  N  K  S  R  K  H  T  E  A  L  Q  K  L  R  
isotig351       660 CCCCGACCTTCATCAAGAAaCAAAAGCAGAAAGCATACCGAGGCACTCCAGAAGTTGAGG

isotig351       240 E  V  N  K  R  L  Q  D  L  R  S  C  L  S  P  K  Q  H  Q  S  
isotig351       720 GAAGTGAACAAGCGCCTCCAAGATCTCCGATCCTGCCTGAGCCCCAAGCAGCACCAGAGT

isotig351       260 P  A  L  Q  N  P  D  D  E  V  V  L  V  D  G  P  V  L  S  Q  
isotig351       780 CCAGCCCTTCAGAACCCAGATGATGAGGTGGTCCTCGTGGACGGGCCTGtCTTGTCACAG

isotig351       280 S  P  R  L  F  T  L  K  I  R  C  R  A  D  L  V  R  L  P  V  
isotig351       840 AGCCCGAGACTCTTCACCCTCAAGATCCGGTGCCGGGCTGACCTAGTCAGATTGCCCGTC

isotig351       300 M  T  S  E  P  L  Q  N  V  V  D  Y  M  A  N  H  L  G  V  S  
isotig351       900 ATGACATCGGAACCCCTTCAGAATGTGGTGGATTACATGGCCAATCATCTTGGGGTGTCT

isotig351       320 P  S  R  I  L  L  L  F  G  E  T  E  L  S  P  T  A  T  P  R  
isotig351       960 CCAAGCAGGATTCTTTTACTCTTTGGAGAGACAGAACTGTCCCCTACTGCCACCCCTAGG

isotig351       340 T  L  K  L  G  V  A  D  I  I  D  C  V  V  L  T  S  S  S  E  
isotig351      1020 ACCCTAAAGCTTGGTGTGGCTGACATCATTGATTGTGTGGTGCTGACAAGTTCTTCAGAG

isotig351       360 A  T  E  T  T  Q  Q  L  C  L  R  V  Q  G  K  E  K  H  Q  M  
isotig351      1080 GCCACAGAGACAACCCAGCAGCTCTGCCTCCGGGTGCAGGGGAAGGAGAAGCACCAGATG

isotig351       380 L  E  I  S  L  S  P  D  S  P  L  E  V  L  M  A  H  Y  E  E  
isotig351      1140 TTGGAGATCTCACTCTCTCCTGACTCtCCTCTTGAGGTCCTCATGGCGCACTATGAGGAG

isotig351       400 A  M  G  L  S  G  H  K  L  S  F  F  F  D  G  T  K  L  S  G  
isotig351      1200 GCCATGGGACTCTCCGGACACAAGCTCTCCTTCTTCTTCGATGGGACAAAGCTGTCGGGC

isotig351       420 K  E  L  P  A  D  L  G  M  E  S  G  D  L  I  E  V  W  G  S  
isotig351      1260 AAGGAGCTGCCAGCTGATCTGGGCATGGAATCCGGGGATCTCATTGAAGTTTGGGGCAGC

isotig351       440 F  L  L  L  F  G  C  R  A  K  T  W  G  Q  Q  L  P  L  L  L  
isotig351      1320 TTCCTCCTCCTGTTTGGATGCAGAGCCAAGACTTGGGGACAACAGCTCCCACTTTtATTA

isotig351       460 L  F  F  A  P  G  L  T  E  T  E  L  E  L  V  Y  L  F  P  A  
isotig351      1380 TTATTTTTtGCCCcAGGGCTAACAGAAACCGAATTAGAACTCGTTTATTTATTTCCGGCA

isotig351       480 L  G  I  E  P  R  A  V  H  M  L  R  M  C  E  V  E  V  K  P  
isotig351      1440 CTGGGGATTGAACCCAGGGCTGTGCATATGCTAAGGATGTGTGAAGTTGAGGTAAAaCCA

isotig351       500 R  H  D  L  C  P  V  S  L  T  V  V  S  S  G  L  I  G  N  C  
isotig351      1500 AGGCATGACCTTTGCCcTGTCTCGTTGACCGTAGTCTCAAGCGGTCTGATTGGTAATTGT

isotig351       520 V  T  V  A  A  L  G  G  L  W  L  C  V  L  V  L  C  T  A  A  
isotig351      1560 GTGACTGTGGCTGCCCTGGGTGgCCTGTGGCTGTGTGTGTTGGTGCTGTGTACAGCAGCT

isotig351       540 P  G  A  W  R  R  G  I  G  F  P  T  I  S  F  S  S  A  Q  M  
isotig351      1620 CCTGGGGCATGGAGAAGGGGTATTGGCTTCCCTACCATTTCGTTCAGTAGTGCACAAATG

isotig351       560 S  L  A  F  G  C  P  G  L  F  M  P  H  W  G  P  T  G  I  L  
isotig351      1680 AGCCTTGCATTTGGGTGCCCAGGCTTGTTTATGCCACATTGGGGACCAACAGGGATTTTA

isotig351       580 I  L  I  W  G  L  R  C  R  Y  L  P  W  W  G  S  V  A  G  K  
isotig351      1740 ATTCTCATTTGGGGGCTGAGATGCAGGTACCTTCCCTGGTGGGGATCGGTTGCAGGGAAA

isotig351       600 T  S  N  D  I  Y  A  E  A  E  D  V  D  S  G  F  L  K  A  N  
isotig351      1800 ACAAGCAATGACATCTATGCTGAAGCTGAGGACGTAGACAGTGGGTTTTTAAAGGCTAAC

isotig351       620 R  S  G  V  F  S  P  G  H  P  L  S  Q  D  S  A  S  I  W  Y  
isotig351      1860 AGGAGTGGTGTCTTCAGCCCTGGGCATCCACTCTCCCAGGACTCGGCCAGCATCTGGTAC

isotig351       640 I  L  P  G  M  H  I  P  H  L  S  T  C  S  C  V  C  L  L  F  
isotig351      1920 ATACTTCCTGGCATGCACATCCCACATCTGAGCACATGCAGCTGTGTTTGTTTACTCTTC

isotig351       660 R  P  P  A  L  S  C  Q  H  P  L  G  I  F  V  S  E  C  L  Y  
isotig351      1980 cGTCCTCCCGCCCTCTCCTGTCAGCACCCACTTGGTATATTTGTATCTGAATGTCTTTAT

isotig351       680 K  M  I  F  M  C  V  I  L  V  Y  V  H  G  T  V  Q  T  F  C  
isotig351      2040 AAGATGATTTTCATGTGTGTGATTTTAGTGTATGTACATGGTACTGTGCAAACATTCTGT

isotig351       700 L  H  S  V  V  T  G  P  C  H  L  L  D  L  L  L  F  R  S  A  
isotig351      2100 CTTCACTCAGTGGTGACAGGCCCATGTCACCTTCTAGATCTGTTGTTGTTCCGATCTGCT

isotig351       720 V  A  A  P  W  H  M  I  L  L  T  H  C  L  N  W  R  D  T  V  
isotig351      2160 GTGGCTGCCCCATGGCATATGATATTACTCACACATTGCCTTAATTGGAGGGATACCGTC

isotig351       740 A  S  S  E  P  N  S  R  P  Q  Q  T  S  F  C  V  F  P  S  G  
isotig351      2220 GCCAGCTCCGAACCAAATTCCAGACCACAGCAAACATCTTTTTGTGTGTTCCCTTCTGGG

isotig351       760 P  G  V  I  V  W  H  T  C  P  G  W  D  C  G  L  R  R  F  I  
isotig351      2280 CCAGGGGTAATTGTCTGGCATACTTGCCCAGGATGGGACTgTGGGTTACGCAGGTTCATT

isotig351       780 S  L  V  F  L  G  F  S  S  E  C  G  L  L  F  L  E  L  L  F  
isotig351      2340 TcTCTGGTTTTTCTAGGgTTTTCTTCAGAATGTGGACTGCTTTTCTTGGAGCTTCTcTTT

isotig351       800 P  S  L  D  Q  Y  L  V  L  L  Y  F  L  I  C  Q  S  T  V  Y  
isotig351      2400 CCTTcTCTTGACCAGTATTTAGTGTTGttgTACTTTCTGATTTGCCAGTCTACTGTGTAT

isotig351       820 K  A  L  H  I  L  I  S  F  I  P  P  F  L  I  N  V  H  L  L  
isotig351      2460 AAAGCACTGCATATCTTAATATCCTTTATCCCACCATTTCTTATCAATGTCCACCTTCTG

isotig351       840 V  F  L  C  L  L  V  F  L  G  F  L  L  C  V  F  S  V  R  S  
isotig351      2520 GTTTTTCTTTGTCTGCTTGTTTTCTTGGGCTTCCTTTTGTGTGTTTTTtCTGTGAGGAGT

isotig351       860 C  L  C  S  R  Q  L  Q  I  T  F  R  I  A  L  C  V  P  R  G  
isotig351      2580 TGCTTGTGTTCTaGgCAATTGCAAATAACCTTCAGAATAGCACTTTGTGTACCCAGGGGG

isotig351       880 I  F  T  K  Q  T  F  L  T  L  M  Q  T  A  G  F  C  K  G  F  
isotig351      2640 ATTTTCACCAAACAGACATTCTTAACTTTAATGCAGACAGCTGGCTTTTGTAAAGGGTTT

isotig351       900 F  V  L  C  F  F  F  F  C  F  C  F  C  F  L  R  E  N  E  K  
isotig351      2700 TTTGTTTTGTGTTTtTTTTTtttttGCTTTTGTTTTtGcTTTTTAAGGGAAAATGAGAAA

isotig351       920 E  L  P  E  W  Q  G  F  R  G  V  S  G  L  L  L  L  D  L  T  
isotig351      2760 GAACTTCCAGAATGGCAGGGGTTCCGGGGAGTCTCAGGATTGCTACTTTTGGATCTCACT

isotig351       940 S  E  T  F  I  V  F  C  L  A  S  F  L  F  F  C  F  Y  L  S  
isotig351      2820 AGTGAAACTTTCATTGTCTTCTGTCTTGCTAGTTTTTTGTTCTTTTGTTTTTATCTCTCA

isotig351       960 Y  D  H  M  R  I  E  F  L  I  C  C  G  V  D  T  D  C  S  E  
isotig351      2880 TATGACCACATGAGAATAGAGTTCCTGATCTGCTGTGGTGTGGATaCTGACTGTTCTGAa

isotig351       980 L  S  T  L  L  L  T  G  Y  S  T  V  L  V  P  D  Y  C  M  L  
isotig351      2940 CTTTCCACTCTGCTTCTCACTGGTTACAGCACTGTACTGGTTCCTGACTATTGTATGTtA

isotig351      1000 R  L  N  H  L  K  C  T  F  V  W  E  S  K  F  Y  C  V  L  I  
isotig351      3000 CGTCTAAACCATCTGAAATGCACCTTTGTGTGGGAGAGCAAATTTTATTGTGTTTTAATT

isotig351      1020 I  M  S  Q  F  S  K  L  C  L  L  K  N  Y  V  V  L  S  Q  E  
isotig351      3060 ATAATGAGCCAGTTTTCtAAGCTTTGTCTaCTAAAAAATTATGTAGTCCTTTCACAAGAA

isotig351      1040 V  C  Q  L  L  K  N  F  F  C  E  N  M  L  Y  L  I  L  I  F  
isotig351      3120 GTATGTCAACTTCTtAAAAATTTTTTTtGtGAGAATATGTTATACCTTATTTTAATATTT

isotig351      1060 K  N  S  M  T  Y  S  F  Q  V  S  Q  L  L  Y  D  N  I  T  H  
isotig351      3180 AAAAATTCCATGACATATAGCTTCCAGGTTTCTCAGCTCTTGTATGACAATATCACACAT

isotig351      1080 Y  Y  Y  V  F  N  L  S  Q  R  E  M  P  L   1094
isotig351      3240 TaCTATTATGTATTCAATCTCAGCCAAAGGGAGATGCCTTTA 3282
""",
        )
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[1]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1104], [0, 3312]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig351         0 E  R  Q  G  R  W  C  V  P  G  E  A  V  E  A  R  V  S  R  S  
isotig351         0 GAAAGGCAGGGTCGctGGTGCGTGCCCGGCGAGGCTGTGGAGGCCcgTGTGTCTAGAAGC

isotig351        20 C  V  R  E  M  A  E  P  G  R  R  R  G  P  R  S  R  G  G  G  
isotig351        60 TGTGTGAGAGAGATGGCGGAACCCGGGAGGAGACGGGGTCCGAGGTCCCGCGGTGGCGGC

isotig351        40 A  G  R  G  A  R  R  A  R  V  A  R  G  R  R  P  R  A  P  Q  
isotig351       120 GCCGGCCGAGGCgCTCGAAGAGCCCGGGTCGCCCGTGGCCGGCGTCCTCGCGCCCCGCAG

isotig351        60 S  L  S  R  L  I  P  D  T  V  L  V  D  L  V  S  D  S  D  E  
isotig351       180 TCTCTGTCCCGGCTCATTCCAGACACGGTGCTTGTGGACTTGGTCAGTGACAGCGACGAG

isotig351        80 E  I  L  E  V  V  A  D  P  V  E  A  P  A  A  R  A  P  A  P  
isotig351       240 GAGATCCTGGAAGTCGTCGCGGACCCGGTAGAAGCGCCCGCCGCCCGGGCcCCcGCGCCG

isotig351       100 A  A  H  G  Q  D  S  D  S  D  S  A  G  A  D  E  G  P  A  G  
isotig351       300 GCCGCACATGGGCAGGACAGCGACAGCGACAGTGCAGGGGCGgACGAGGGGCCTGCAGGA

isotig351       120 A  P  Q  T  L  V  R  R  R  R  R  R  L  L  D  P  G  E  A  P  
isotig351       360 GCCCCTCAGACCTTGGTCCGGCGGCGGCGCCGGCGGCTGCTGGATCCCGGCGAGGCACCG

isotig351       140 V  V  P  V  Y  S  G  K  V  Q  S  S  L  N  L  I  P  D  N  S  
isotig351       420 GTGGTTCCTGTGTACTCCGGGAAGGTACAAAGCAGCCTCAACCTCATCCCAGATAATTCA

isotig351       160 S  L  L  K  L  C  P  S  E  P  E  D  E  A  D  V  T  D  C  G  
isotig351       480 TCCCTCTTGAAACTTTGCCCCTCAGAGCCTGAAGATGAGGCAGATGTGACAGATTGTGGC

isotig351       180 S  P  P  P  E  D  A  L  I  P  G  S  P  W  K  K  K  L  R  N  
isotig351       540 AGTCCTCCTCCTGAGGATGCCCTAATTCCAGGTTCTCCCTGGAAGAAGAAGCTGAGGAAT

isotig351       200 K  H  E  K  E  E  M  K  M  E  E  F  P  D  Q  D  I  S  P  L  
isotig351       600 AAGCATGAAAAaGAAGAGATGAAGATGGAAGAGTTtCCGGACCAGGACATCTCTCCTTTG

isotig351       220 P  R  P  S  S  R  N  K  S  R  K  H  T  E  A  L  Q  K  L  R  
isotig351       660 CCCCGACCTTCATCAAGAAaCAAAAGCAGAAAGCATACCGAGGCACTCCAGAAGTTGAGG

isotig351       240 E  V  N  K  R  L  Q  D  L  R  S  C  L  S  P  K  Q  H  Q  S  
isotig351       720 GAAGTGAACAAGCGCCTCCAAGATCTCCGATCCTGCCTGAGCCCCAAGCAGCACCAGAGT

isotig351       260 P  A  L  Q  N  P  D  D  E  V  V  L  V  D  G  P  V  L  S  Q  
isotig351       780 CCAGCCCTTCAGAACCCAGATGATGAGGTGGTCCTCGTGGACGGGCCTGtCTTGTCACAG

isotig351       280 S  P  R  L  F  T  L  K  I  R  C  R  A  D  L  V  R  L  P  V  
isotig351       840 AGCCCGAGACTCTTCACCCTCAAGATCCGGTGCCGGGCTGACCTAGTCAGATTGCCCGTC

isotig351       300 M  T  S  E  P  L  Q  N  V  V  D  Y  M  A  N  H  L  G  V  S  
isotig351       900 ATGACATCGGAACCCCTTCAGAATGTGGTGGATTACATGGCCAATCATCTTGGGGTGTCT

isotig351       320 P  S  R  I  L  L  L  F  G  E  T  E  L  S  P  T  A  T  P  R  
isotig351       960 CCAAGCAGGATTCTTTTACTCTTTGGAGAGACAGAACTGTCCCCTACTGCCACCCCTAGG

isotig351       340 T  L  K  L  G  V  A  D  I  I  D  C  V  V  L  T  S  S  S  E  
isotig351      1020 ACCCTAAAGCTTGGTGTGGCTGACATCATTGATTGTGTGGTGCTGACAAGTTCTTCAGAG

isotig351       360 A  T  E  T  T  Q  Q  L  C  L  R  V  Q  G  K  E  K  H  Q  M  
isotig351      1080 GCCACAGAGACAACCCAGCAGCTCTGCCTCCGGGTGCAGGGGAAGGAGAAGCACCAGATG

isotig351       380 L  E  I  S  L  S  P  D  S  P  L  E  V  L  M  A  H  Y  E  E  
isotig351      1140 TTGGAGATCTCACTCTCTCCTGACTCgCCTCTTGAGGTCCTCATGGCGCACTATGAGGAG

isotig351       400 A  M  G  L  S  G  H  K  L  S  F  F  F  D  G  T  K  L  S  G  
isotig351      1200 GCCATGGGACTCTCCGGACACAAGCTCTCCTTCTTCTTCGATGGGACAAAGCTGTCGGGC

isotig351       420 K  E  L  P  A  D  L  G  M  E  S  G  D  L  I  E  V  W  G  S  
isotig351      1260 AAGGAACTGCCAGCTGATCTGGGCATGGAATCCGGGGATCTCATTGAAGTTTGGGGCAGC

isotig351       440 F  L  L  L  F  G  C  R  A  K  T  W  G  Q  Q  L  P  L  L  L  
isotig351      1320 TTCCTCCTCCTGTTTGGATGCAGAGCCAAGACTTGGGGACAACAGCTCCCACTTTTATTA

isotig351       460 L  F  F  A  P  G  L  T  E  T  E  L  E  L  V  Y  L  F  P  A  
isotig351      1380 TTATTTTTTGCCCCAGGGCTAACAGAAACCGAATTAGAACTCGTTTATTTATTTCCGGCA

isotig351       480 L  G  I  E  P  W  A  V  H  M  L  R  M  C  E  V  E  V  K  P  
isotig351      1440 CTGGGGATTGAACCCTGGGCTGTGCATATGCTAAGGATGTGTGAAGTTGAGGTAAAACCA

isotig351       500 R  H  D  L  C  P  V  S  L  T  D  W  L  C  D  C  G  C  P  G  
isotig351      1500 AGGCATGACCTTTGCCCTGTCTCGTTGACCGATTGGTTGTGTGACTGTGGCTGCCCTGGG

isotig351       520 W  P  V  A  V  C  V  G  A  V  D  S  S  S  W  G  M  E  K  G  
isotig351      1560 TGGCCTGTGGCTGTGTGTGTTGGTGCTGTGGACAGCAGCTCCTGGGGCATGGAGAAGGGG

isotig351       540 Y  W  L  P  Y  H  F  V  Q  C  T  N  E  P  C  I  W  V  P  R  
isotig351      1620 TATTGGCTTCCCTACCATTTCGTTCAGTGCACAAATGAGCCTTGCATTTGGGTGCCCAGG

isotig351       560 L  V  I  Y  A  T  L  G  T  N  R  D  F  N  S  H  L  G  A  E  
isotig351      1680 CTTGTGATTTATGCCACATTGGGGACCAACAGGGATTTTAATTCTCATTTGGGGGCTGAG

isotig351       580 M  Q  V  P  S  L  V  G  I  V  G  C  R  E  I  N  K  Q  H  L  
isotig351      1740 ATGCAGGTACCTTCCCTGGTGGGGATAGTCGGTTGCAGGGAAATAAACAAGCAACATCTA

isotig351       600 C  S  G  L  G  R  Q  W  V  I  F  K  G  Q  E  W  C  L  Q  P  
isotig351      1800 TGCAGCGGACTAGGTAGACAGTGGGTAATTTTTAAAGGCCAGGAGTGGTGTCTTCAGCCC

isotig351       620 W  A  S  T  L  P  G  L  G  Q  H  L  V  H  T  S  W  H  A  H  
isotig351      1860 TGGGCATCCACTCTCCCAGGACTCGGCCAGCATCTGGTACATACTTCCTGGCATGCACAT

isotig351       640 L  A  T  S  E  H  M  Q  L  L  S  L  M  F  T  L  V  P  S  S  
isotig351      1920 CTAGCCACATCTGAGCACATGCAGCTGTTGAGTTTGATGTTTACTCTAGTTCcGTCCTCC

isotig351       660 R  P  L  L  S  A  P  T  W  Y  I  C  I  M  S  L  D  D  F  L  
isotig351      1980 CGCCCTCTCCTGTCAGCACCCACTTGGTATATTTGTATCATGTCTTTAGATGATTTTCTG

isotig351       680 N  V  C  D  F  S  V  C  T  W  Y  C  A  I  D  I  L  S  S  L  
isotig351      2040 AATGTGTGTGATTTTAGTGTATGTACATGGTACTGTGCAATAGACATTCTGTCTTCACTC

isotig351       700 S  V  S  D  R  L  A  M  L  A  P  S  R  S  V  V  V  P  I  C  
isotig351      2100 AGTGTGAGTGACAGGCTAGCCATGTTAGCACCTTCTAGATCTGTTGTTGTTCCGATCTGC

isotig351       720 C  G  C  P  I  V  A  L  D  D  I  T  H  T  L  P  L  E  G  Y  
isotig351      2160 TGTGGCTGCCCCATAGTGGCATTAGATGATATTACTCACACATTGCCTTTGGAGGGATAC

isotig351       740 L  S  R  Q  L  L  G  T  K  F  Q  T  T  A  N  I  F  L  C  V  
isotig351      2220 CTGAGTCGCCAGCTCCTAGGAACCAAATTCCAGACCACAGCAAACATCTTTTTGTGTGTT

isotig351       760 P  F  W  A  R  V  G  N  C  L  A  Y  L  P  R  M  G  L  W  V  
isotig351      2280 CCCTTCTGGGCCAGGGTAGGTAATTGTCTGGCATACTTGCCCAGGATGGGACTgTGGGTT

isotig351       780 T  Q  V  H  F  S  G  F  S  R  V  F  F  R  M  W  T  A  F  L  
isotig351      2340 ACGCAGGTTCATTTcTCTGGTTTTTCTAGGgTTTTCTTCAGAATGTGGACTGCTTTTCTT

isotig351       800 G  A  S  L  S  F  S  P  V  F  S  V  V  V  L  S  D  L  P  V  
isotig351      2400 GGAGCTTCTcTTTCCTTcTCTCCAGTATTTAGTGTTGttgTACTTTCTGATTTGCCAGTC

isotig351       820 Y  C  V  S  T  A  Y  L  N  I  L  Y  L  T  T  I  S  Y  Q  C  
isotig351      2460 TACTGTGTAAGCACTGCATATCTTAATATCCTTTATCTGACCACCATTTCTTATCAATGT

isotig351       840 P  P  S  G  F  S  L  L  A  A  C  F  L  V  R  L  P  F  V  C  
isotig351      2520 CCACCTTCTGGTTTTTCTTTGTTAGCTGCTTGTTTTCTTGTGAGGCTTCCTTTTGTGTGT

isotig351       860 F  F  C  E  E  L  L  V  F  A  I  A  N  N  L  Q  N  S  T  L  
isotig351      2580 TTTTtCTGTGAGGAGTTGCTTGTGTTCgCAATTGCAAATAACCTTCAGAATAGCACTTTG

isotig351       880 C  T  Q  G  D  F  H  Q  T  D  I  L  N  F  N  A  D  S  W  L  
isotig351      2640 TGTACCCAGGGGGATTTTCACCAAACAGACATTCTTAACTTTAATGCAGACAGCTGGCTT

isotig351       900 L  L  E  R  V  F  C  F  V  F  F  F  F  L  L  L  F  L  L  F  
isotig351      2700 TTGTTAGAAAGGGTTTTTTGTTTTGTGTTTtTTTTTtttttGCTTTTGTTTTtGcTTTTT

isotig351       920 K  G  K  E  R  T  S  R  M  A  G  V  L  A  G  S  L  R  I  A  
isotig351      2760 AAGGGAAAAGAAAGAACTTCCAGAATGGCAGGGGTTCTAGCGGGGAGTCTCAGGATTGCT

isotig351       940 T  F  G  S  L  N  L  R  N  F  H  C  L  L  L  A  C  F  F  V  
isotig351      2820 ACTTTTGGATCTCTAAACTTAAGAAACTTTCATTGTCTTCTGTTAGCTTGCTTTTTTGTT

isotig351       960 L  L  F  L  S  L  I  L  R  P  L  N  E  N  R  V  L  T  D  L  
isotig351      2880 CTTTTGTTTTTATCTCTCATATTGAGACCACTAAATGAGAATAGAGTTCTAACTGATCTG

isotig351       980 L  W  C  G  Y  L  F  T  F  H  S  A  S  H  W  L  Q  L  D  C  
isotig351      2940 CTGTGGTGTGGATaCCTGTTCaCTTTCCACTCTGCTTCTCACTGGTTACAGCTAGACTGT

isotig351      1000 T  G  S  L  L  Y  V  T  S  K  P  S  E  M  H  L  C  V  G  E  
isotig351      3000 ACTGGTTCCCTATTGTATGTtACGTCTAAACCATCTGAAATGCACCTTTGTGTGGGAGAG

isotig351      1020 Q  I  L  L  C  F  N  Y  N  E  P  V  F  A  L  S  T  K  K  L  
isotig351      3060 CAAATTTTATTGTGTTTTAATTATAATGAGCCAGTTTTCGCTTTGTCTaCTAAAAAATTA

isotig351      1040 I  C  S  L  T  F  T  R  S  M  S  T  S  K  F  F  L  E  Y  V  
isotig351      3120 ATATGTAGTCTGACTTTCACAAGAAGTATGTCAACTTCTAAATTTTTTTtGGAATATGTT

isotig351      1060 I  P  L  N  F  N  I  K  F  H  D  I  V  L  P  G  F  S  A  L  
isotig351      3180 ATACCTTTGAATTTTAATATTAAATTCCATGACATAGTACTTCCAGGTTTCTCAGCTCTT

isotig351      1080 V  Q  L  M  N  L  N  T  L  L  L  C  I  Q  S  Q  P  I  K  G  
isotig351      3240 GTACAATTAATGAATCTGAACACATTaCTATTATGTATTCAATCTCAGCCAATAAAGGGA

isotig351      1100 D  A  F  N   1104
isotig351      3300 GATGCCTTTAAC 3312
""",
        )
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[2]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 419], [0, 1257]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  A  E  P  V  G  K  R  G  R  W  S  G  G  S  G  A  G  R  G  
ENSG00000         0 ATGGCGGAGCCTGTGGGGAAGCGGGGCCGCTGGTCCGGAGGTAGCGGTGCCGGCCGAGGG

ENSG00000        20 G  R  G  G  W  G  G  R  G  R  R  P  R  A  Q  R  S  P  S  R  
ENSG00000        60 GGTCGGGGCGGCTGGGGCGGTCGGGGCCGGCGTCCTCGGGCCCAGCGGTCTCCATCCCGG

ENSG00000        40 G  T  L  D  V  V  S  V  D  L  V  T  D  S  D  E  E  I  L  E  
ENSG00000       120 GGCACGCTGGACGTAGTGTCTGTGGACTTGGTCACCGACAGCGATGAGGAAATTCTGGAG

ENSG00000        60 V  A  T  A  R  G  A  A  D  E  V  E  V  E  P  P  E  P  P  G  
ENSG00000       180 GTCGCCACCGCTCGCGGTGCCGCGGACGAGGTTGAGGTGGAGCCCCCGGAGCCCCCGGGG

ENSG00000        80 P  V  A  S  R  D  N  S  N  S  D  S  E  G  E  D  R  R  P  A  
ENSG00000       240 CCGGTCGCGTCCCGGGATAACAGCAACAGTGACAGCGAAGGGGAGGACAGGCGGCCCGCA

ENSG00000       100 G  P  P  R  E  P  V  R  R  R  R  R  L  V  L  D  P  G  E  A  
ENSG00000       300 GGACCCCCGCGGGAGCCGGTCAGGCGGCGGCGGCGGCTGGTGCTGGATCCGGGGGAGGCG

ENSG00000       120 P  L  V  P  V  Y  S  G  K  V  K  S  S  L  R  L  I  P  D  D  
ENSG00000       360 CCGCTGGTTCCGGTGTACTCGGGGAAGGTTAAAAGCAGCCTTCGCCTTATCCCAGATGAT

ENSG00000       140 L  S  L  L  K  L  Y  P  P  G  D  E  E  E  A  E  L  A  D  S  
ENSG00000       420 CTATCCCTCCTGAAACTCTACCCTCCAGGGGATGAGGAAGAGGCAGAGCTGGCAGATTCG

ENSG00000       160 S  G  L  Y  H  E  G  S  P  S  P  G  S  P  W  K  T  K  L  R  
ENSG00000       480 AGTGGTCTCTACCATGAGGGCTCCCCATCACCAGGCTCTCCCTGGAAGACAAAGCTGAGG

ENSG00000       180 T  K  D  K  E  E  K  K  K  T  E  F  L  D  L  D  N  S  P  L  
ENSG00000       540 ACTAAGGATAAAGAAGAGAAGAAAAAGACAGAGTTTCTGGATCTGGACAACTCTCCTCTG

ENSG00000       200 S  P  P  S  P  R  T  K  S  R  T  H  T  R  A  L  K  K  L  S  
ENSG00000       600 TCCCCACCTTCACCAAGGACCAAAAGCAGAACGCATACTCGGGCACTCAAGAAGTTAAGT

ENSG00000       220 E  V  N  K  R  L  Q  D  L  R  S  C  L  S  P  K  P  P  Q  G  
ENSG00000       660 GAGGTGAACAAGCGCCTCCAGGATCTCCGTTCCTGTCTGAGCCCCAAGCCACCTCAGGGT

ENSG00000       240 Q  E  Q  Q  G  Q  E  D  E  V  V  L  V  E  G  P  T  L  P  E  
ENSG00000       720 CAAGAGCAACAGGGCCAAGAGGATGAAGTGGTCTTGGTGGAAGGGCCCACCCTCCCAGAG

ENSG00000       260 T  P  R  L  F  P  L  K  I  R  C  R  A  D  L  V  R  L  P  L  
ENSG00000       780 ACCCCCCGACTCTTCCCACTCAAAATCCGTTGCCGGGCTGACCTGGTCAGATTGCCCCTC

ENSG00000       280 R  M  S  E  P  L  Q  S  V  V  D  H  M  A  T  H  L  G  V  S  
ENSG00000       840 AGGATGTCGGAGCCCCTGCAGAGTGTGGTGGACCACATGGCCACCCACCTTGGGGTGTCC

ENSG00000       300 P  S  R  I  L  L  L  F  G  E  T  E  L  S  P  T  A  T  P  R  
ENSG00000       900 CCAAGCAGGATCCTTTTGCTTTTTGGAGAGACAGAGCTATCACCTACTGCCACTCCCAGG

ENSG00000       320 T  L  K  L  G  V  A  D  I  I  D  C  V  V  L  T  S  S  P  E  
ENSG00000       960 ACCCTAAAGCTCGGAGTGGCTGACATCATTGACTGTGTGGTACTAACAAGTTCTCCAGAG

ENSG00000       340 A  T  E  T  S  Q  Q  L  Q  L  R  V  Q  G  K  E  K  H  Q  T  
ENSG00000      1020 GCCACAGAGACGTCCCAACAGCTCCAGCTCCGGGTGCAGGGAAAGGAGAAACACCAGACA

ENSG00000       360 L  E  V  S  L  S  R  D  S  P  L  K  T  L  M  S  H  Y  E  E  
ENSG00000      1080 CTGGAAGTCTCACTGTCTCGAGATTCCCCTCTAAAGACCCTCATGTCCCACTATGAGGAG

ENSG00000       380 A  M  G  L  S  G  R  K  L  S  F  F  F  D  G  T  K  L  S  G  
ENSG00000      1140 GCCATGGGACTGTCGGGACGGAAGCTCTCCTTCTTCTTTGATGGGACAAAGCTTTCAGGC

ENSG00000       400 R  E  L  P  A  D  L  G  M  E  S  G  D  L  I  E  V  W  G  
ENSG00000      1200 AGGGAGCTGCCAGCTGACCTGGGCATGGAATCTGGGGACCTCATTGAGGTCTGGGGC

ENSG00000       419
ENSG00000      1257
""",
        )
        alignment = protein_alignment.mapall(codon_alignments)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
                np.array([[   0,   72,  255,  255,  603,  606, 1317, 1530,
                           1530, 1737, 1737, 1902, 1902, 1926, 1926, 2031,
                           2088, 2343, 2346, 2418, 2442, 2556, 2562, 2763,
                           2784, 2853, 2859, 3165, 3165, 3282, 3282],
                          [   0,   72,  255,  255,  603,  606, 1317, 1530,
                           1569, 1776, 1821, 1986, 1992, 2016, 2031, 2136,
                           2136, 2391, 2391, 2463, 2463, 2577, 2577, 2778,
                           2778, 2847, 2847, 3153, 3162, 3279, 3312],
                          [   0,    0,  183,  198,  546,  546, 1257, 1257,
                           1257, 1257, 1257, 1257, 1257, 1257, 1257, 1257,
                           1257, 1257, 1257, 1257, 1257, 1257, 1257, 1257,
                           1257, 1257, 1257, 1257, 1257, 1257, 1257]])
                # fmt: on
            )
        )
        self.assertEqual(
            format(alignment, "clustal"),
            """\
isotig35100                         GAAAGGCAGGGTCGctGGTGCGTGCCCGGCGAGGCTGTGGAGGCCcgTGT
isotig35101                         GAAAGGCAGGGTCGctGGTGCGTGCCCGGCGAGGCTGTGGAGGCCcgTGT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GTCTAGAAGCTGTGTGAGAGAGATGGCGGAACCCGGGAGGAGACGGGGTC
isotig35101                         GTCTAGAAGCTGTGTGAGAGAGATGGCGGAACCCGGGAGGAGACGGGGTC
ENSG00000176953:ENST0000032080      ----------------------ATGGCGGAGCCTGTGGGGAAGCGGGGCC

isotig35100                         CGAGGTCCCGCGGTGGCGGCGCCGGCCGAGGCgCTCGAAGAGCCCGGGTC
isotig35101                         CGAGGTCCCGCGGTGGCGGCGCCGGCCGAGGCgCTCGAAGAGCCCGGGTC
ENSG00000176953:ENST0000032080      GCTGGTCCGGAGGTAGCGGTGCCGGCCGAGGGGGTCGGGGCGGCTGGGGC

isotig35100                         GCCCGTGGCCGGCGTCCTCGCGCCCCGCAGTCTCTGTCCCGGCTCATTCC
isotig35101                         GCCCGTGGCCGGCGTCCTCGCGCCCCGCAGTCTCTGTCCCGGCTCATTCC
ENSG00000176953:ENST0000032080      GGTCGGGGCCGGCGTCCTCGGGCCCAGCGGTCTCCATCCCGGGGCACGCT

isotig35100                         AGACACGGTGCTTGTGGACTTGGTCAGTGACAGCGACGAGGAGATCCTGG
isotig35101                         AGACACGGTGCTTGTGGACTTGGTCAGTGACAGCGACGAGGAGATCCTGG
ENSG00000176953:ENST0000032080      GGACGTAGTGTCTGTGGACTTGGTCACCGACAGCGATGAGGAAATTCTGG

isotig35100                         AAGTC---------------GTCGCGGACCCGGTAGAAGCGCCCGCCGCC
isotig35101                         AAGTC---------------GTCGCGGACCCGGTAGAAGCGCCCGCCGCC
ENSG00000176953:ENST0000032080      AGGTCGCCACCGCTCGCGGTGCCGCGGACGAGGTTGAGGTGGAGCCCCCG

isotig35100                         CGGGCcCCcGCGCCGGCCGCACATGGGCAGGACAGCGACAGCGACAGTGC
isotig35101                         CGGGCcCCcGCGCCGGCCGCACATGGGCAGGACAGCGACAGCGACAGTGC
ENSG00000176953:ENST0000032080      GAGCCCCCGGGGCCGGTCGCGTCCCGGGATAACAGCAACAGTGACAGCGA

isotig35100                         AGGGGCGgACGAGGGGCCTGCAGGAGCCCCTCAGACCTTGGTCCGGCGGC
isotig35101                         AGGGGCGgACGAGGGGCCTGCAGGAGCCCCTCAGACCTTGGTCCGGCGGC
ENSG00000176953:ENST0000032080      AGGGGAGGACAGGCGGCCCGCAGGACCCCCGCGGGAGCCGGTCAGGCGGC

isotig35100                         GGCGCCGGCGGCTGCTGGATCCCGGCGAGGCACCGGTGGTTCCTGTGTAC
isotig35101                         GGCGCCGGCGGCTGCTGGATCCCGGCGAGGCACCGGTGGTTCCTGTGTAC
ENSG00000176953:ENST0000032080      GGCGGCGGCTGGTGCTGGATCCGGGGGAGGCGCCGCTGGTTCCGGTGTAC

isotig35100                         TCCGGGAAGGTACAAAGCAGCCTCAACCTCATCCCAGATAATTCATCCCT
isotig35101                         TCCGGGAAGGTACAAAGCAGCCTCAACCTCATCCCAGATAATTCATCCCT
ENSG00000176953:ENST0000032080      TCGGGGAAGGTTAAAAGCAGCCTTCGCCTTATCCCAGATGATCTATCCCT

isotig35100                         CTTGAAACTTTGCCCCTCAGAGCCTGAAGATGAGGCAGATGTGACAGATT
isotig35101                         CTTGAAACTTTGCCCCTCAGAGCCTGAAGATGAGGCAGATGTGACAGATT
ENSG00000176953:ENST0000032080      CCTGAAACTCTACCCTCCAGGGGATGAGGAAGAGGCAGAGCTGGCAGATT

isotig35100                         GTGGCAGTCCTCCTCCTGAGGATGCCCTAATTCCAGGTTCTCCCTGGAAG
isotig35101                         GTGGCAGTCCTCCTCCTGAGGATGCCCTAATTCCAGGTTCTCCCTGGAAG
ENSG00000176953:ENST0000032080      CGAGTGGTCTCTACCATGAGGGCTCCCCATCACCAGGCTCTCCCTGGAAG

isotig35100                         AAGAAGCTGAGGAATAAGCATGAAAAaGAAGAGATGAAGATGGAAGAGTT
isotig35101                         AAGAAGCTGAGGAATAAGCATGAAAAaGAAGAGATGAAGATGGAAGAGTT
ENSG00000176953:ENST0000032080      ACAAAGCTGAGGACTAAG---GATAAAGAAGAGAAGAAAAAGACAGAGTT

isotig35100                         tCCGGACCAGGACATCTCTCCTTTGCCCCGACCTTCATCAAGAAaCAAAA
isotig35101                         tCCGGACCAGGACATCTCTCCTTTGCCCCGACCTTCATCAAGAAaCAAAA
ENSG00000176953:ENST0000032080      TCTGGATCTGGACAACTCTCCTCTGTCCCCACCTTCACCAAGGACCAAAA

isotig35100                         GCAGAAAGCATACCGAGGCACTCCAGAAGTTGAGGGAAGTGAACAAGCGC
isotig35101                         GCAGAAAGCATACCGAGGCACTCCAGAAGTTGAGGGAAGTGAACAAGCGC
ENSG00000176953:ENST0000032080      GCAGAACGCATACTCGGGCACTCAAGAAGTTAAGTGAGGTGAACAAGCGC

isotig35100                         CTCCAAGATCTCCGATCCTGCCTGAGCCCCAAGCAGCACCAGAGTCCAGC
isotig35101                         CTCCAAGATCTCCGATCCTGCCTGAGCCCCAAGCAGCACCAGAGTCCAGC
ENSG00000176953:ENST0000032080      CTCCAGGATCTCCGTTCCTGTCTGAGCCCCAAGCCACCTCAGGGTCAAGA

isotig35100                         CCTTCAGAACCCAGATGATGAGGTGGTCCTCGTGGACGGGCCTGtCTTGT
isotig35101                         CCTTCAGAACCCAGATGATGAGGTGGTCCTCGTGGACGGGCCTGtCTTGT
ENSG00000176953:ENST0000032080      GCAACAGGGCCAAGAGGATGAAGTGGTCTTGGTGGAAGGGCCCACCCTCC

isotig35100                         CACAGAGCCCGAGACTCTTCACCCTCAAGATCCGGTGCCGGGCTGACCTA
isotig35101                         CACAGAGCCCGAGACTCTTCACCCTCAAGATCCGGTGCCGGGCTGACCTA
ENSG00000176953:ENST0000032080      CAGAGACCCCCCGACTCTTCCCACTCAAAATCCGTTGCCGGGCTGACCTG

isotig35100                         GTCAGATTGCCCGTCATGACATCGGAACCCCTTCAGAATGTGGTGGATTA
isotig35101                         GTCAGATTGCCCGTCATGACATCGGAACCCCTTCAGAATGTGGTGGATTA
ENSG00000176953:ENST0000032080      GTCAGATTGCCCCTCAGGATGTCGGAGCCCCTGCAGAGTGTGGTGGACCA

isotig35100                         CATGGCCAATCATCTTGGGGTGTCTCCAAGCAGGATTCTTTTACTCTTTG
isotig35101                         CATGGCCAATCATCTTGGGGTGTCTCCAAGCAGGATTCTTTTACTCTTTG
ENSG00000176953:ENST0000032080      CATGGCCACCCACCTTGGGGTGTCCCCAAGCAGGATCCTTTTGCTTTTTG

isotig35100                         GAGAGACAGAACTGTCCCCTACTGCCACCCCTAGGACCCTAAAGCTTGGT
isotig35101                         GAGAGACAGAACTGTCCCCTACTGCCACCCCTAGGACCCTAAAGCTTGGT
ENSG00000176953:ENST0000032080      GAGAGACAGAGCTATCACCTACTGCCACTCCCAGGACCCTAAAGCTCGGA

isotig35100                         GTGGCTGACATCATTGATTGTGTGGTGCTGACAAGTTCTTCAGAGGCCAC
isotig35101                         GTGGCTGACATCATTGATTGTGTGGTGCTGACAAGTTCTTCAGAGGCCAC
ENSG00000176953:ENST0000032080      GTGGCTGACATCATTGACTGTGTGGTACTAACAAGTTCTCCAGAGGCCAC

isotig35100                         AGAGACAACCCAGCAGCTCTGCCTCCGGGTGCAGGGGAAGGAGAAGCACC
isotig35101                         AGAGACAACCCAGCAGCTCTGCCTCCGGGTGCAGGGGAAGGAGAAGCACC
ENSG00000176953:ENST0000032080      AGAGACGTCCCAACAGCTCCAGCTCCGGGTGCAGGGAAAGGAGAAACACC

isotig35100                         AGATGTTGGAGATCTCACTCTCTCCTGACTCtCCTCTTGAGGTCCTCATG
isotig35101                         AGATGTTGGAGATCTCACTCTCTCCTGACTCgCCTCTTGAGGTCCTCATG
ENSG00000176953:ENST0000032080      AGACACTGGAAGTCTCACTGTCTCGAGATTCCCCTCTAAAGACCCTCATG

isotig35100                         GCGCACTATGAGGAGGCCATGGGACTCTCCGGACACAAGCTCTCCTTCTT
isotig35101                         GCGCACTATGAGGAGGCCATGGGACTCTCCGGACACAAGCTCTCCTTCTT
ENSG00000176953:ENST0000032080      TCCCACTATGAGGAGGCCATGGGACTGTCGGGACGGAAGCTCTCCTTCTT

isotig35100                         CTTCGATGGGACAAAGCTGTCGGGCAAGGAGCTGCCAGCTGATCTGGGCA
isotig35101                         CTTCGATGGGACAAAGCTGTCGGGCAAGGAACTGCCAGCTGATCTGGGCA
ENSG00000176953:ENST0000032080      CTTTGATGGGACAAAGCTTTCAGGCAGGGAGCTGCCAGCTGACCTGGGCA

isotig35100                         TGGAATCCGGGGATCTCATTGAAGTTTGGGGCAGCTTCCTCCTCCTGTTT
isotig35101                         TGGAATCCGGGGATCTCATTGAAGTTTGGGGCAGCTTCCTCCTCCTGTTT
ENSG00000176953:ENST0000032080      TGGAATCTGGGGACCTCATTGAGGTCTGGGGC------------------

isotig35100                         GGATGCAGAGCCAAGACTTGGGGACAACAGCTCCCACTTTtATTATTATT
isotig35101                         GGATGCAGAGCCAAGACTTGGGGACAACAGCTCCCACTTTTATTATTATT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TTTtGCCCcAGGGCTAACAGAAACCGAATTAGAACTCGTTTATTTATTTC
isotig35101                         TTTTGCCCCAGGGCTAACAGAAACCGAATTAGAACTCGTTTATTTATTTC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         CGGCACTGGGGATTGAACCCAGGGCTGTGCATATGCTAAGGATGTGTGAA
isotig35101                         CGGCACTGGGGATTGAACCCTGGGCTGTGCATATGCTAAGGATGTGTGAA
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GTTGAGGTAAAaCCAAGGCATGACCTTTGCCcTGTCTCGTTGACC-----
isotig35101                         GTTGAGGTAAAACCAAGGCATGACCTTTGCCCTGTCTCGTTGACCGATTG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         ----------------------------------GTAGTCTCAAGCGGTC
isotig35101                         GTTGTGTGACTGTGGCTGCCCTGGGTGGCCTGTGGCTGTGTGTGTTGGTG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TGATTGGTAATTGTGTGACTGTGGCTGCCCTGGGTGgCCTGTGGCTGTGT
isotig35101                         CTGTGGACAGCAGCTCCTGGGGCATGGAGAAGGGGTATTGGCTTCCCTAC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GTGTTGGTGCTGTGTACAGCAGCTCCTGGGGCATGGAGAAGGGGTATTGG
isotig35101                         CATTTCGTTCAGTGCACAAATGAGCCTTGCATTTGGGTGCCCAGGCTTGT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         CTTCCCTACCATTTCGTTCAGTAGTGCACAAATGAGCCTTGCATTTGGGT
isotig35101                         GATTTATGCCACATTGGGGACCAACAGGGATTTTAATTCTCATTTGGGGG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GCCCAGGCTTGTTTATGCCACATTGGGGACCAACAGGGATT---------
isotig35101                         CTGAGATGCAGGTACCTTCCCTGGTGGGGATAGTCGGTTGCAGGGAAATA
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         ------------------------------------TTAATTCTCATTTG
isotig35101                         AACAAGCAACATCTATGCAGCGGACTAGGTAGACAGTGGGTAATTTTTAA
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GGGGCTGAGATGCAGGTACCTTCCCTGGTGGGGATCGGTTGCAGGGAAAA
isotig35101                         AGGCCAGGAGTGGTGTCTTCAGCCCTGGGCATCCACTCTCCCAGGACTCG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         CAAGCAATGACATCTATGCTGAAGCTGAGGACGTAGACAGTGGGTTTTTA
isotig35101                         GCCAGCATCTGGTACATACTTCCTGGCATGCACATCTAGCCACATCTGAG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         AAGGCTAACAGGAGTGGTGTCTTCAGCCCTGGGCATCCACTCTCCCAGGA
isotig35101                         CACATGCAGCTGTTGAGTTTGATGTTTACTCTAGTTCcGTCCTCCCGCCC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         C------TCGGCCAGCATCTGGTACATACTT---------------CCTG
isotig35101                         TCTCCTGTCAGCACCCACTTGGTATATTTGTATCATGTCTTTAGATGATT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GCATGCACATCCCACATCTGAGCACATGCAGCTGTGTTTGTTTACTCTTC
isotig35101                         TTCTGAATGTGTGTGATTTTAGTGTATGTACATGGTACTGTGCAATAGAC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         cGTCCTCCCGCCCTCTCCTGTCAGCACCCACTTGGTATATTTGTATCTGA
isotig35101                         ATTCTGTCTTCACTCAGTGTGAGTGACAGGCTAGCCATGTTAGCACCTTC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         ATGTCTTTATAAGATGATTTTCATGTGTGTGATTTTAGTGTATGTACATG
isotig35101                         T-------------------------------------------------
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GTACTGTGCAAACATTCTGTCTTCACTCAGTGGTGACAGGCCCATGTCAC
isotig35101                         --------AGATCTGTTGTTGTTCCGATCTGCTGTGGCTGCCCCATAGTG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         CTTCTAGATCTGTTGTTGTTCCGATCTGCTGTGGCTGCCCCATGGCATAT
isotig35101                         GCATTAGATGATATTACTCACACATTGCCTTTGGAGGGATACCTGAGTCG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GATATTACTCACACATTGCCTTAATTGGAGGGATACCGTCGCCAGCTCCG
isotig35101                         CCAGCTCCTAGGAACCAAATTCCAGACCACAGCAAACATCTTTTTGTGTG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         AACCAAATTCCAGACCACAGCAAACATCTTTTTGTGTGTTCCCTTCTGGG
isotig35101                         TTCCCTTCTGGGCCAGGGTAGGTAATTGTCTGGCATACTTGCCCAGGATG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         CCAGGGGTAATTGTCTGGCATACTTGCCCAGGATGGGACTgTGGGTTACG
isotig35101                         GGACTgTGGGTTACGCAGGTTCATTTcTCTGGTTTTTCTAGGgTTTTCTT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         CAGGTTCATTTcTCTGGTTTTTCTAGGgTTTTCTTCAGAATGTGGACTGC
isotig35101                         CAGAATGTGGACT---GCTTTTCTTGGAGCTTCTcTTTCCTTcTCTCCAG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TTTTCTTGGAGCTTCTcTTTCCTTcTCTTGACCAGTATTTAGTGTTGttg
isotig35101                         TATTTAGTGTTGttgTACTTTCTGATTTGCCAGTCTAC------------
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TACTTTCTGATTTGCCAGTCTACTGTGTATAAAGCACTGCATATCTTAAT
isotig35101                         ------------TGTGTAAGCACTGCATATCTTAATATCCTTTATCTGAC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         ATCCTTTATCCCACCATTTCTTATCAATGTCCACCTTCTGGTTTTTCTTT
isotig35101                         CACCATTTCTTATCAATGTCCACCTTCTGGTTTTTCTTTGTTAGCTGCTT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GTCTGCTTGTTTTCTTGGGCTTCCTTTTGTGTGTTTTTtCTGTGAGGAGT
isotig35101                         GTTTTCTTGTGAGGCTTCCTTTTGTG------TGTTTTTtCTGTGAGGAG
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TGCTTGTGTTCTaGgCAATTGCAAATAACCTTCAGAATAGCACTTTGTGT
isotig35101                         TTGCTTGTGTTCgCAATTGCAAATAACCTTCAGAATAGCACTTTGTGTAC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         ACCCAGGGGGATTTTCACCAAACAGACATTCTTAACTTTAATGCAGACAG
isotig35101                         CCAGGGGGATTTTCACCAAACAGACATTCTTAACTTTAATGCAGACAGCT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         CTGGCTTTTGTAAAGGGTTTTTTGTTTTGTGTTTtTTTTTtttttGCTTT
isotig35101                         GGCTTTTGTTAGAAAGGGTTTTTTGTTTTGTGTTTtTTTTTtttttGCTT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TGTTTTtGcTTTTTAAGGGAAAATGAGAAAGAACTTCCAGAATGGCAGGG
isotig35101                         TTGTTTTtGcTTTTTAAGGGAAAAGAAAGAACT-----------------
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GTTCCGGGGAGTCTCAGGATTGCTACTTTTGGATCTCACTAGTGAAACTT
isotig35101                         ----TCCAGAATGGCAGGGGTTCTAGCGGGGAGTCTCAGGATTGCTACTT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TCATTGTCTTCTGTCTTGCTAGTTTTTTGTTCTTTTGTTTTTATCTCTCA
isotig35101                         TTGGATCTCTAAACTTAAGAAAC------TTTCATTGTCTTCTGTTAGCT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TATGACCACATGAGAATAGAGTTCCTGATCTGCTGTGGTGTGGATaCTGA
isotig35101                         TGCTTTTTTGTTCTTTTGTTTTTATCTCTCATATTGAGACCACTAAATGA
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         CTGTTCTGAaCTTTCCACTCTGCTTCTCACTGGTTACAGCACTGTACTGG
isotig35101                         GAATAGAGTTCTAACTGATCTGCTGTGGTGTGGATaCCTGTTCaCTTTCC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TTCCTGACTATTGTATGTtACGTCTAAACCATCTGAAATGCACCTTTGTG
isotig35101                         ACTCTGCTTCTCACTGGTTACAGCTAGACTGTACTGGTTCCCTATTGTAT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TGGGAGAGCAAATTTTATTGTGTTTTAATTATAATGAGCCAGTTTTCtAA
isotig35101                         GTtACGTCTAAACCATCTGAAATGCACCTTTGTGTGGGAGAGCAAATTTT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GCTTTGTCTaCTAAAAAATTATGTAGTCCTTTCACAAGAAGTATGTCAAC
isotig35101                         ATTGTGTTTTAATTATAATGAGCCAGTTTTCGCTTTGTCTaCTAAAAAAT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TTCTtAAAAATTTTTTTtGtGAGAATATGTTATAC---------CTTATT
isotig35101                         TAATATGTAGTCTGACTTTCACAAGAAGTATGTCAACTTCTAAATTTTTT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         TTAATATTTAAAAATTCCATGACATATAGCTTCCAGGTTTCTCAGCTCTT
isotig35101                         TtGGAATATGTTATACCTTTGAATTTTAATATTAAATTCCATGACATAGT
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         GTATGACAATATCACACATTaCTATTATGTATTCAATCTCAGCCAAAGGG
isotig35101                         ACTTCCAGGTTTCTCAGCTCTTGTACAATTAATGAATCTGAACACATTaC
ENSG00000176953:ENST0000032080      --------------------------------------------------

isotig35100                         AGATGCCTTTA---------------------------------
isotig35101                         TATTATGTATTCAATCTCAGCCAATAAAGGGAGATGCCTTTAAC
ENSG00000176953:ENST0000032080      --------------------------------------------


""",
        )

    def test3(self):
        aligner = CodonAligner()
        nucleotide_records = SeqIO.index("codonalign/nucl3.fa", "fasta")
        protein_alignment = Align.read("codonalign/pro3.aln", "clustal")
        self.assertEqual(len(protein_alignment.sequences), 10)
        codon_alignments = []
        protein_record = protein_alignment.sequences[0]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 847], [0, 2541]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  S  L  W  G  L  V  S  K  M  P  P  E  K  V  Q  R  L  Y  V  
ENSG00000         0 ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC

ENSG00000        20 D  F  P  Q  H  L  R  H  L  L  G  D  W  L  E  S  Q  P  W  E  
ENSG00000        60 GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG

ENSG00000        40 F  L  V  G  S  D  A  F  C  C  N  L  A  S  A  L  L  S  D  T  
ENSG00000       120 TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT

ENSG00000        60 V  Q  H  L  Q  A  S  V  G  E  Q  G  E  G  S  T  I  L  Q  H  
ENSG00000       180 GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC

ENSG00000        80 I  S  T  L  E  S  I  Y  Q  R  D  P  L  K  L  V  A  T  F  R  
ENSG00000       240 ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA

ENSG00000       100 Q  I  L  Q  G  E  K  K  A  V  M  E  Q  F  R  H  L  P  M  P  
ENSG00000       300 CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT

ENSG00000       120 F  H  W  K  Q  E  E  L  K  F  K  T  G  L  R  R  L  Q  H  R  
ENSG00000       360 TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA

ENSG00000       140 V  G  E  I  H  L  L  R  E  A  L  Q  K  G  A  E  A  G  Q  V  
ENSG00000       420 GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG

ENSG00000       160 S  L  H  S  L  I  E  T  P  A  N  G  T  G  P  S  E  A  L  A  
ENSG00000       480 TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC

ENSG00000       180 M  L  L  Q  E  T  T  G  E  L  E  A  A  K  A  L  V  L  K  R  
ENSG00000       540 ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG

ENSG00000       200 I  Q  I  W  K  R  Q  Q  Q  L  A  G  N  G  A  P  F  E  E  S  
ENSG00000       600 ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC

ENSG00000       220 L  A  P  L  Q  E  R  C  E  S  L  V  D  I  Y  S  Q  L  Q  Q  
ENSG00000       660 CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG

ENSG00000       240 E  V  G  A  A  G  G  E  L  E  P  K  T  R  A  S  L  T  G  R  
ENSG00000       720 GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG

ENSG00000       260 L  D  E  V  L  R  T  L  V  T  S  C  F  L  V  E  K  Q  P  P  
ENSG00000       780 CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC

ENSG00000       280 Q  V  L  K  T  Q  T  K  F  Q  A  G  V  R  F  L  L  G  L  R  
ENSG00000       840 CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG

ENSG00000       300 F  L  G  A  P  A  K  P  P  L  V  R  A  D  M  V  T  E  K  Q  
ENSG00000       900 TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG

ENSG00000       320 A  R  E  L  S  V  P  Q  G  P  G  A  G  A  E  S  T  G  E  I  
ENSG00000       960 GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC

ENSG00000       340 I  N  N  T  V  P  L  E  N  S  I  P  G  N  C  C  S  A  L  F  
ENSG00000      1020 ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC

ENSG00000       360 K  N  L  L  L  K  K  I  K  R  C  E  R  K  G  T  E  S  V  T  
ENSG00000      1080 AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA

ENSG00000       380 E  E  K  C  A  V  L  F  S  A  S  F  T  L  G  P  G  K  L  P  
ENSG00000      1140 GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC

ENSG00000       400 I  Q  L  Q  A  L  S  L  P  L  V  V  I  V  H  G  N  Q  D  N  
ENSG00000      1200 ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC

ENSG00000       420 N  A  K  A  T  I  L  W  D  N  A  F  S  E  M  D  R  V  P  F  
ENSG00000      1260 AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT

ENSG00000       440 V  V  A  E  R  V  P  W  E  K  M  C  E  T  L  N  L  K  F  M  
ENSG00000      1320 GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG

ENSG00000       460 A  E  V  G  T  N  R  G  L  L  P  E  H  F  L  F  L  A  Q  K  
ENSG00000      1380 GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG

ENSG00000       480 I  F  N  D  N  S  L  S  M  E  A  F  Q  H  R  S  V  S  W  S  
ENSG00000      1440 ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG

ENSG00000       500 Q  F  N  K  E  I  L  L  G  R  G  F  T  F  W  Q  W  F  D  G  
ENSG00000      1500 CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT

ENSG00000       520 V  L  D  L  T  K  R  C  L  R  S  Y  W  S  D  R  L  I  I  G  
ENSG00000      1560 GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC

ENSG00000       540 F  I  S  K  Q  Y  V  T  S  L  L  L  N  E  P  D  G  T  F  L  
ENSG00000      1620 TTCATCAGCAAACAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTC

ENSG00000       560 L  R  F  S  D  S  E  I  G  G  I  T  I  A  H  V  I  R  G  Q  
ENSG00000      1680 CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG

ENSG00000       580 D  G  S  P  Q  I  E  N  I  Q  P  F  S  A  K  D  L  S  I  R  
ENSG00000      1740 GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC

ENSG00000       600 S  L  G  D  R  I  R  D  L  A  Q  L  K  N  L  Y  P  K  K  P  
ENSG00000      1800 TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC

ENSG00000       620 K  D  E  A  F  R  S  H  Y  K  P  E  Q  M  G  K  D  G  R  G  
ENSG00000      1860 AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT

ENSG00000       640 Y  V  P  A  T  I  K  M  T  V  E  R  D  Q  P  L  P  T  P  E  
ENSG00000      1920 TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG

ENSG00000       660 L  Q  M  P  T  M  V  P  S  Y  D  L  G  M  A  P  D  S  S  M  
ENSG00000      1980 CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG

ENSG00000       680 S  M  Q  L  G  P  D  M  V  P  Q  V  Y  P  P  H  S  H  S  I  
ENSG00000      2040 AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC

ENSG00000       700 P  P  Y  Q  G  L  S  P  E  E  S  V  N  V  L  S  A  F  Q  E  
ENSG00000      2100 CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG

ENSG00000       720 P  H  L  Q  M  P  P  S  L  G  Q  M  S  L  P  F  D  Q  P  H  
ENSG00000      2160 CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC

ENSG00000       740 P  Q  G  L  L  P  C  Q  P  Q  E  H  A  V  S  S  P  D  P  L  
ENSG00000      2220 CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG

ENSG00000       760 L  C  S  D  V  T  M  V  E  D  S  C  L  S  Q  P  V  T  A  F  
ENSG00000      2280 CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT

ENSG00000       780 P  Q  G  T  W  I  G  E  D  I  F  P  P  L  L  P  P  T  E  Q  
ENSG00000      2340 CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG

ENSG00000       800 D  L  T  K  L  L  L  E  G  Q  G  E  S  G  G  G  S  L  G  A  
ENSG00000      2400 GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA

ENSG00000       820 Q  P  L  L  Q  P  S  H  Y  G  Q  S  G  I  S  M  S  H  M  D  
ENSG00000      2460 CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC

ENSG00000       840 L  R  A  N  P  S  W    847
ENSG00000      2520 CTAAGGGCCAACCCCAGTTGG 2541
""",
        )
        protein_record = protein_alignment.sequences[1]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 847], [0, 2541]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  S  L  W  G  L  V  S  K  M  P  P  E  K  V  Q  R  L  Y  V  
ENSG00000         0 ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC

ENSG00000        20 D  F  P  Q  H  L  R  H  L  L  G  D  W  L  E  S  Q  P  W  E  
ENSG00000        60 GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG

ENSG00000        40 F  L  V  G  S  D  A  F  C  C  N  L  A  S  A  L  L  S  D  T  
ENSG00000       120 TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT

ENSG00000        60 V  Q  H  L  Q  A  S  V  G  E  Q  G  E  G  S  T  I  L  Q  H  
ENSG00000       180 GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC

ENSG00000        80 I  S  T  L  E  S  I  Y  Q  R  D  P  L  K  L  V  A  T  F  R  
ENSG00000       240 ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA

ENSG00000       100 Q  I  L  Q  G  E  K  K  A  V  M  E  Q  F  R  H  L  P  M  P  
ENSG00000       300 CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT

ENSG00000       120 F  H  W  K  Q  E  E  L  K  F  K  T  G  L  R  R  L  Q  H  R  
ENSG00000       360 TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA

ENSG00000       140 V  G  E  I  H  L  L  R  E  A  L  Q  K  G  A  E  A  G  Q  V  
ENSG00000       420 GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG

ENSG00000       160 S  L  H  S  L  I  E  T  P  A  N  G  T  G  P  S  E  A  L  A  
ENSG00000       480 TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC

ENSG00000       180 M  L  L  Q  E  T  T  G  E  L  E  A  A  K  A  L  V  L  K  R  
ENSG00000       540 ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG

ENSG00000       200 I  Q  I  W  K  R  Q  Q  Q  L  A  G  N  G  A  P  F  E  E  S  
ENSG00000       600 ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC

ENSG00000       220 L  A  P  L  Q  E  R  C  E  S  L  V  D  I  Y  S  Q  L  Q  Q  
ENSG00000       660 CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG

ENSG00000       240 E  V  G  A  A  G  G  E  L  E  P  K  T  R  A  S  L  T  G  R  
ENSG00000       720 GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG

ENSG00000       260 L  D  E  V  L  R  T  L  V  T  S  C  F  L  V  E  K  Q  P  P  
ENSG00000       780 CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC

ENSG00000       280 Q  V  L  K  T  Q  T  K  F  Q  A  G  V  R  F  L  L  G  L  R  
ENSG00000       840 CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG

ENSG00000       300 F  L  G  A  P  A  K  P  P  L  V  R  A  D  M  V  T  E  K  Q  
ENSG00000       900 TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG

ENSG00000       320 A  R  E  L  S  V  P  Q  G  P  G  A  G  A  E  S  T  G  E  I  
ENSG00000       960 GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC

ENSG00000       340 I  N  N  T  V  P  L  E  N  S  I  P  G  N  C  C  S  A  L  F  
ENSG00000      1020 ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC

ENSG00000       360 K  N  L  L  L  K  K  I  K  R  C  E  R  K  G  T  E  S  V  T  
ENSG00000      1080 AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA

ENSG00000       380 E  E  K  C  A  V  L  F  S  A  S  F  T  L  G  P  G  K  L  P  
ENSG00000      1140 GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC

ENSG00000       400 I  Q  L  Q  A  L  S  L  P  L  V  V  I  V  H  G  N  Q  D  N  
ENSG00000      1200 ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC

ENSG00000       420 N  A  K  A  T  I  L  W  D  N  A  F  S  E  M  D  R  V  P  F  
ENSG00000      1260 AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT

ENSG00000       440 V  V  A  E  R  V  P  W  E  K  M  C  E  T  L  N  L  K  F  M  
ENSG00000      1320 GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG

ENSG00000       460 A  E  V  G  T  N  R  G  L  L  P  E  H  F  L  F  L  A  Q  K  
ENSG00000      1380 GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG

ENSG00000       480 I  F  N  D  N  S  L  S  M  E  A  F  Q  H  R  S  V  S  W  S  
ENSG00000      1440 ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG

ENSG00000       500 Q  F  N  K  E  I  L  L  G  R  G  F  T  F  W  Q  W  F  D  G  
ENSG00000      1500 CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT

ENSG00000       520 V  L  D  L  T  K  R  C  L  R  S  Y  W  S  D  R  L  I  I  G  
ENSG00000      1560 GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC

ENSG00000       540 F  I  S  K  Q  Y  V  T  S  L  L  L  N  E  P  D  G  T  F  L  
ENSG00000      1620 TTCATCAGCAAACAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTC

ENSG00000       560 L  R  F  S  D  S  E  I  G  G  I  T  I  A  H  V  I  R  G  Q  
ENSG00000      1680 CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG

ENSG00000       580 D  G  S  P  Q  I  E  N  I  Q  P  F  S  A  K  D  L  S  I  R  
ENSG00000      1740 GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC

ENSG00000       600 S  L  G  D  R  I  R  D  L  A  Q  L  K  N  L  Y  P  K  K  P  
ENSG00000      1800 TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC

ENSG00000       620 K  D  E  A  F  R  S  H  Y  K  P  E  Q  M  G  K  D  G  R  G  
ENSG00000      1860 AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT

ENSG00000       640 Y  V  P  A  T  I  K  M  T  V  E  R  D  Q  P  L  P  T  P  E  
ENSG00000      1920 TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG

ENSG00000       660 L  Q  M  P  T  M  V  P  S  Y  D  L  G  M  A  P  D  S  S  M  
ENSG00000      1980 CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG

ENSG00000       680 S  M  Q  L  G  P  D  M  V  P  Q  V  Y  P  P  H  S  H  S  I  
ENSG00000      2040 AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC

ENSG00000       700 P  P  Y  Q  G  L  S  P  E  E  S  V  N  V  L  S  A  F  Q  E  
ENSG00000      2100 CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG

ENSG00000       720 P  H  L  Q  M  P  P  S  L  G  Q  M  S  L  P  F  D  Q  P  H  
ENSG00000      2160 CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC

ENSG00000       740 P  Q  G  L  L  P  C  Q  P  Q  E  H  A  V  S  S  P  D  P  L  
ENSG00000      2220 CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG

ENSG00000       760 L  C  S  D  V  T  M  V  E  D  S  C  L  S  Q  P  V  T  A  F  
ENSG00000      2280 CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT

ENSG00000       780 P  Q  G  T  W  I  G  E  D  I  F  P  P  L  L  P  P  T  E  Q  
ENSG00000      2340 CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG

ENSG00000       800 D  L  T  K  L  L  L  E  G  Q  G  E  S  G  G  G  S  L  G  A  
ENSG00000      2400 GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA

ENSG00000       820 Q  P  L  L  Q  P  S  H  Y  G  Q  S  G  I  S  M  S  H  M  D  
ENSG00000      2460 CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC

ENSG00000       840 L  R  A  N  P  S  W    847
ENSG00000      2520 CTAAGGGCCAACCCCAGTTGG 2541
""",
        )
        protein_record = protein_alignment.sequences[2]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 847], [0, 2541]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  S  L  W  G  L  V  S  K  M  P  P  E  K  V  Q  R  L  Y  V  
ENSG00000         0 ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC

ENSG00000        20 D  F  P  Q  H  L  R  H  L  L  G  D  W  L  E  S  Q  P  W  E  
ENSG00000        60 GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG

ENSG00000        40 F  L  V  G  S  D  A  F  C  C  N  L  A  S  A  L  L  S  D  T  
ENSG00000       120 TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT

ENSG00000        60 V  Q  H  L  Q  A  S  V  G  E  Q  G  E  G  S  T  I  L  Q  H  
ENSG00000       180 GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC

ENSG00000        80 I  S  T  L  E  S  I  Y  Q  R  D  P  L  K  L  V  A  T  F  R  
ENSG00000       240 ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA

ENSG00000       100 Q  I  L  Q  G  E  K  K  A  V  M  E  Q  F  R  H  L  P  M  P  
ENSG00000       300 CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT

ENSG00000       120 F  H  W  K  Q  E  E  L  K  F  K  T  G  L  R  R  L  Q  H  R  
ENSG00000       360 TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA

ENSG00000       140 V  G  E  I  H  L  L  R  E  A  L  Q  K  G  A  E  A  G  Q  V  
ENSG00000       420 GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG

ENSG00000       160 S  L  H  S  L  I  E  T  P  A  N  G  T  G  P  S  E  A  L  A  
ENSG00000       480 TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC

ENSG00000       180 M  L  L  Q  E  T  T  G  E  L  E  A  A  K  A  L  V  L  K  R  
ENSG00000       540 ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG

ENSG00000       200 I  Q  I  W  K  R  Q  Q  Q  L  A  G  N  G  A  P  F  E  E  S  
ENSG00000       600 ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC

ENSG00000       220 L  A  P  L  Q  E  R  C  E  S  L  V  D  I  Y  S  Q  L  Q  Q  
ENSG00000       660 CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG

ENSG00000       240 E  V  G  A  A  G  G  E  L  E  P  K  T  R  A  S  L  T  G  R  
ENSG00000       720 GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG

ENSG00000       260 L  D  E  V  L  R  T  L  V  T  S  C  F  L  V  E  K  Q  P  P  
ENSG00000       780 CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC

ENSG00000       280 Q  V  L  K  T  Q  T  K  F  Q  A  G  V  R  F  L  L  G  L  R  
ENSG00000       840 CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG

ENSG00000       300 F  L  G  A  P  A  K  P  P  L  V  R  A  D  M  V  T  E  K  Q  
ENSG00000       900 TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG

ENSG00000       320 A  R  E  L  S  V  P  Q  G  P  G  A  G  A  E  S  T  G  E  I  
ENSG00000       960 GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC

ENSG00000       340 I  N  N  T  V  P  L  E  N  S  I  P  G  N  C  C  S  A  L  F  
ENSG00000      1020 ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC

ENSG00000       360 K  N  L  L  L  K  K  I  K  R  C  E  R  K  G  T  E  S  V  T  
ENSG00000      1080 AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA

ENSG00000       380 E  E  K  C  A  V  L  F  S  A  S  F  T  L  G  P  G  K  L  P  
ENSG00000      1140 GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC

ENSG00000       400 I  Q  L  Q  A  L  S  L  P  L  V  V  I  V  H  G  N  Q  D  N  
ENSG00000      1200 ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC

ENSG00000       420 N  A  K  A  T  I  L  W  D  N  A  F  S  E  M  D  R  V  P  F  
ENSG00000      1260 AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT

ENSG00000       440 V  V  A  E  R  V  P  W  E  K  M  C  E  T  L  N  L  K  F  M  
ENSG00000      1320 GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG

ENSG00000       460 A  E  V  G  T  N  R  G  L  L  P  E  H  F  L  F  L  A  Q  K  
ENSG00000      1380 GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG

ENSG00000       480 I  F  N  D  N  S  L  S  M  E  A  F  Q  H  R  S  V  S  W  S  
ENSG00000      1440 ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG

ENSG00000       500 Q  F  N  K  E  I  L  L  G  R  G  F  T  F  W  Q  W  F  D  G  
ENSG00000      1500 CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT

ENSG00000       520 V  L  D  L  T  K  R  C  L  R  S  Y  W  S  D  R  L  I  I  G  
ENSG00000      1560 GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC

ENSG00000       540 F  I  S  K  Q  Y  V  T  S  L  L  L  N  E  P  D  G  T  F  L  
ENSG00000      1620 TTCATCAGCAAACAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTC

ENSG00000       560 L  R  F  S  D  S  E  I  G  G  I  T  I  A  H  V  I  R  G  Q  
ENSG00000      1680 CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG

ENSG00000       580 D  G  S  P  Q  I  E  N  I  Q  P  F  S  A  K  D  L  S  I  R  
ENSG00000      1740 GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC

ENSG00000       600 S  L  G  D  R  I  R  D  L  A  Q  L  K  N  L  Y  P  K  K  P  
ENSG00000      1800 TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC

ENSG00000       620 K  D  E  A  F  R  S  H  Y  K  P  E  Q  M  G  K  D  G  R  G  
ENSG00000      1860 AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT

ENSG00000       640 Y  V  P  A  T  I  K  M  T  V  E  R  D  Q  P  L  P  T  P  E  
ENSG00000      1920 TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG

ENSG00000       660 L  Q  M  P  T  M  V  P  S  Y  D  L  G  M  A  P  D  S  S  M  
ENSG00000      1980 CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG

ENSG00000       680 S  M  Q  L  G  P  D  M  V  P  Q  V  Y  P  P  H  S  H  S  I  
ENSG00000      2040 AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC

ENSG00000       700 P  P  Y  Q  G  L  S  P  E  E  S  V  N  V  L  S  A  F  Q  E  
ENSG00000      2100 CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG

ENSG00000       720 P  H  L  Q  M  P  P  S  L  G  Q  M  S  L  P  F  D  Q  P  H  
ENSG00000      2160 CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC

ENSG00000       740 P  Q  G  L  L  P  C  Q  P  Q  E  H  A  V  S  S  P  D  P  L  
ENSG00000      2220 CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG

ENSG00000       760 L  C  S  D  V  T  M  V  E  D  S  C  L  S  Q  P  V  T  A  F  
ENSG00000      2280 CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT

ENSG00000       780 P  Q  G  T  W  I  G  E  D  I  F  P  P  L  L  P  P  T  E  Q  
ENSG00000      2340 CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG

ENSG00000       800 D  L  T  K  L  L  L  E  G  Q  G  E  S  G  G  G  S  L  G  A  
ENSG00000      2400 GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA

ENSG00000       820 Q  P  L  L  Q  P  S  H  Y  G  Q  S  G  I  S  M  S  H  M  D  
ENSG00000      2460 CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC

ENSG00000       840 L  R  A  N  P  S  W    847
ENSG00000      2520 CTAAGGGCCAACCCCAGTTGG 2541
""",
        )
        protein_record = protein_alignment.sequences[3]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 847], [0, 2541]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  S  L  W  G  L  V  S  K  M  P  P  E  K  V  Q  R  L  Y  V  
ENSG00000         0 ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC

ENSG00000        20 D  F  P  Q  H  L  R  H  L  L  G  D  W  L  E  S  Q  P  W  E  
ENSG00000        60 GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG

ENSG00000        40 F  L  V  G  S  D  A  F  C  C  N  L  A  S  A  L  L  S  D  T  
ENSG00000       120 TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT

ENSG00000        60 V  Q  H  L  Q  A  S  V  G  E  Q  G  E  G  S  T  I  L  Q  H  
ENSG00000       180 GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC

ENSG00000        80 I  S  T  L  E  S  I  Y  Q  R  D  P  L  K  L  V  A  T  F  R  
ENSG00000       240 ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA

ENSG00000       100 Q  I  L  Q  G  E  K  K  A  V  M  E  Q  F  R  H  L  P  M  P  
ENSG00000       300 CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT

ENSG00000       120 F  H  W  K  Q  E  E  L  K  F  K  T  G  L  R  R  L  Q  H  R  
ENSG00000       360 TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA

ENSG00000       140 V  G  E  I  H  L  L  R  E  A  L  Q  K  G  A  E  A  G  Q  V  
ENSG00000       420 GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG

ENSG00000       160 S  L  H  S  L  I  E  T  P  A  N  G  T  G  P  S  E  A  L  A  
ENSG00000       480 TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC

ENSG00000       180 M  L  L  Q  E  T  T  G  E  L  E  A  A  K  A  L  V  L  K  R  
ENSG00000       540 ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG

ENSG00000       200 I  Q  I  W  K  R  Q  Q  Q  L  A  G  N  G  A  P  F  E  E  S  
ENSG00000       600 ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC

ENSG00000       220 L  A  P  L  Q  E  R  C  E  S  L  V  D  I  Y  S  Q  L  Q  Q  
ENSG00000       660 CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG

ENSG00000       240 E  V  G  A  A  G  G  E  L  E  P  K  T  R  A  S  L  T  G  R  
ENSG00000       720 GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG

ENSG00000       260 L  D  E  V  L  R  T  L  V  T  S  C  F  L  V  E  K  Q  P  P  
ENSG00000       780 CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC

ENSG00000       280 Q  V  L  K  T  Q  T  K  F  Q  A  G  V  R  F  L  L  G  L  R  
ENSG00000       840 CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG

ENSG00000       300 F  L  G  A  P  A  K  P  P  L  V  R  A  D  M  V  T  E  K  Q  
ENSG00000       900 TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG

ENSG00000       320 A  R  E  L  S  V  P  Q  G  P  G  A  G  A  E  S  T  G  E  I  
ENSG00000       960 GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC

ENSG00000       340 I  N  N  T  V  P  L  E  N  S  I  P  G  N  C  C  S  A  L  F  
ENSG00000      1020 ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC

ENSG00000       360 K  N  L  L  L  K  K  I  K  R  C  E  R  K  G  T  E  S  V  T  
ENSG00000      1080 AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA

ENSG00000       380 E  E  K  C  A  V  L  F  S  A  S  F  T  L  G  P  G  K  L  P  
ENSG00000      1140 GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC

ENSG00000       400 I  Q  L  Q  A  L  S  L  P  L  V  V  I  V  H  G  N  Q  D  N  
ENSG00000      1200 ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC

ENSG00000       420 N  A  K  A  T  I  L  W  D  N  A  F  S  E  M  D  R  V  P  F  
ENSG00000      1260 AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT

ENSG00000       440 V  V  A  E  R  V  P  W  E  K  M  C  E  T  L  N  L  K  F  M  
ENSG00000      1320 GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG

ENSG00000       460 A  E  V  G  T  N  R  G  L  L  P  E  H  F  L  F  L  A  Q  K  
ENSG00000      1380 GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG

ENSG00000       480 I  F  N  D  N  S  L  S  M  E  A  F  Q  H  R  S  V  S  W  S  
ENSG00000      1440 ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG

ENSG00000       500 Q  F  N  K  E  I  L  L  G  R  G  F  T  F  W  Q  W  F  D  G  
ENSG00000      1500 CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT

ENSG00000       520 V  L  D  L  T  K  R  C  L  R  S  Y  W  S  D  R  L  I  I  G  
ENSG00000      1560 GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC

ENSG00000       540 F  I  S  K  Q  Y  V  T  S  L  L  L  N  E  P  D  G  T  F  L  
ENSG00000      1620 TTCATCAGCAAACAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTC

ENSG00000       560 L  R  F  S  D  S  E  I  G  G  I  T  I  A  H  V  I  R  G  Q  
ENSG00000      1680 CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG

ENSG00000       580 D  G  S  P  Q  I  E  N  I  Q  P  F  S  A  K  D  L  S  I  R  
ENSG00000      1740 GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC

ENSG00000       600 S  L  G  D  R  I  R  D  L  A  Q  L  K  N  L  Y  P  K  K  P  
ENSG00000      1800 TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC

ENSG00000       620 K  D  E  A  F  R  S  H  Y  K  P  E  Q  M  G  K  D  G  R  G  
ENSG00000      1860 AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT

ENSG00000       640 Y  V  P  A  T  I  K  M  T  V  E  R  D  Q  P  L  P  T  P  E  
ENSG00000      1920 TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG

ENSG00000       660 L  Q  M  P  T  M  V  P  S  Y  D  L  G  M  A  P  D  S  S  M  
ENSG00000      1980 CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG

ENSG00000       680 S  M  Q  L  G  P  D  M  V  P  Q  V  Y  P  P  H  S  H  S  I  
ENSG00000      2040 AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC

ENSG00000       700 P  P  Y  Q  G  L  S  P  E  E  S  V  N  V  L  S  A  F  Q  E  
ENSG00000      2100 CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG

ENSG00000       720 P  H  L  Q  M  P  P  S  L  G  Q  M  S  L  P  F  D  Q  P  H  
ENSG00000      2160 CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC

ENSG00000       740 P  Q  G  L  L  P  C  Q  P  Q  E  H  A  V  S  S  P  D  P  L  
ENSG00000      2220 CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG

ENSG00000       760 L  C  S  D  V  T  M  V  E  D  S  C  L  S  Q  P  V  T  A  F  
ENSG00000      2280 CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT

ENSG00000       780 P  Q  G  T  W  I  G  E  D  I  F  P  P  L  L  P  P  T  E  Q  
ENSG00000      2340 CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG

ENSG00000       800 D  L  T  K  L  L  L  E  G  Q  G  E  S  G  G  G  S  L  G  A  
ENSG00000      2400 GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA

ENSG00000       820 Q  P  L  L  Q  P  S  H  Y  G  Q  S  G  I  S  M  S  H  M  D  
ENSG00000      2460 CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC

ENSG00000       840 L  R  A  N  P  S  W    847
ENSG00000      2520 CTAAGGGCCAACCCCAGTTGG 2541
""",
        )
        protein_record = protein_alignment.sequences[4]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 737], [0, 2211]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  E  Q  F  R  H  L  P  M  P  F  H  W  K  Q  E  E  L  K  F  
ENSG00000         0 ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGAACTCAAGTTT

ENSG00000        20 K  T  G  L  R  R  L  Q  H  R  V  G  E  I  H  L  L  R  E  A  
ENSG00000        60 AAGACAGGCTTGCGGAGGCTGCAGCACCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCC

ENSG00000        40 L  Q  K  G  A  E  A  G  Q  V  S  L  H  S  L  I  E  T  P  A  
ENSG00000       120 CTGCAGAAGGGGGCTGAGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCT

ENSG00000        60 N  G  T  G  P  S  E  A  L  A  M  L  L  Q  E  T  T  G  E  L  
ENSG00000       180 AATGGGACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGAGCTA

ENSG00000        80 E  A  A  K  A  L  V  L  K  R  I  Q  I  W  K  R  Q  Q  Q  L  
ENSG00000       240 GAGGCAGCCAAAGCCCTAGTGCTGAAGAGGATCCAGATTTGGAAACGGCAGCAGCAGCTG

ENSG00000       100 A  G  N  G  A  P  F  E  E  S  L  A  P  L  Q  E  R  C  E  S  
ENSG00000       300 GCAGGGAATGGCGCACCGTTTGAGGAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGC

ENSG00000       120 L  V  D  I  Y  S  Q  L  Q  Q  E  V  G  A  A  G  G  E  L  E  
ENSG00000       360 CTGGTGGACATTTATTCCCAGCTACAGCAGGAGGTAGGGGCGGCTGGTGGGGAGCTTGAG

ENSG00000       140 P  K  T  R  A  S  L  T  G  R  L  D  E  V  L  R  T  L  V  T  
ENSG00000       420 CCCAAGACCCGGGCATCGCTGACTGGCCGGCTGGATGAAGTCCTGAGAACCCTCGTCACC

ENSG00000       160 S  C  F  L  V  E  K  Q  P  P  Q  V  L  K  T  Q  T  K  F  Q  
ENSG00000       480 AGTTGCTTCCTGGTGGAGAAGCAGCCCCCCCAGGTACTGAAGACTCAGACCAAGTTCCAG

ENSG00000       180 A  G  V  R  F  L  L  G  L  R  F  L  G  A  P  A  K  P  P  L  
ENSG00000       540 GCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTCCTGGGGGCCCCAGCCAAGCCTCCGCTG

ENSG00000       200 V  R  A  D  M  V  T  E  K  Q  A  R  E  L  S  V  P  Q  G  P  
ENSG00000       600 GTCAGGGCCGACATGGTGACAGAGAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCT

ENSG00000       220 G  A  G  A  E  S  T  G  E  I  I  N  N  T  V  P  L  E  N  S  
ENSG00000       660 GGGGCTGGAGCAGAAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGC

ENSG00000       240 I  P  G  N  C  C  S  A  L  F  K  N  L  L  L  K  K  I  K  R  
ENSG00000       720 ATTCCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGATCAAGCGG

ENSG00000       260 C  E  R  K  G  T  E  S  V  T  E  E  K  C  A  V  L  F  S  A  
ENSG00000       780 TGTGAGCGGAAGGGCACTGAGTCTGTCACAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCC

ENSG00000       280 S  F  T  L  G  P  G  K  L  P  I  Q  L  Q  A  L  S  L  P  L  
ENSG00000       840 AGCTTCACACTTGGCCCCGGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTG

ENSG00000       300 V  V  I  V  H  G  N  Q  D  N  N  A  K  A  T  I  L  W  D  N  
ENSG00000       900 GTGGTCATCGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACAAT

ENSG00000       320 A  F  S  E  M  D  R  V  P  F  V  V  A  E  R  V  P  W  E  K  
ENSG00000       960 GCCTTCTCTGAGATGGACCGCGTGCCCTTTGTGGTGGCTGAGCGGGTGCCCTGGGAGAAG

ENSG00000       340 M  C  E  T  L  N  L  K  F  M  A  E  V  G  T  N  R  G  L  L  
ENSG00000      1020 ATGTGTGAAACTCTGAACCTGAAGTTCATGGCTGAGGTGGGGACCAACCGGGGGCTGCTC

ENSG00000       360 P  E  H  F  L  F  L  A  Q  K  I  F  N  D  N  S  L  S  M  E  
ENSG00000      1080 CCAGAGCACTTCCTCTTCCTGGCCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAG

ENSG00000       380 A  F  Q  H  R  S  V  S  W  S  Q  F  N  K  E  I  L  L  G  R  
ENSG00000      1140 GCCTTCCAGCACCGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGT

ENSG00000       400 G  F  T  F  W  Q  W  F  D  G  V  L  D  L  T  K  R  C  L  R  
ENSG00000      1200 GGCTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCTGTCTCCGG

ENSG00000       420 S  Y  W  S  D  R  L  I  I  G  F  I  S  K  Q  Y  V  T  S  L  
ENSG00000      1260 AGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAACAGTACGTTACTAGCCTT

ENSG00000       440 L  L  N  E  P  D  G  T  F  L  L  R  F  S  D  S  E  I  G  G  
ENSG00000      1320 CTTCTCAATGAGCCCGACGGAACCTTTCTCCTCCGCTTCAGCGACTCAGAGATTGGGGGC

ENSG00000       460 I  T  I  A  H  V  I  R  G  Q  D  G  S  P  Q  I  E  N  I  Q  
ENSG00000      1380 ATCACCATTGCCCATGTCATCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAG

ENSG00000       480 P  F  S  A  K  D  L  S  I  R  S  L  G  D  R  I  R  D  L  A  
ENSG00000      1440 CCATTCTCTGCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGATCTTGCT

ENSG00000       500 Q  L  K  N  L  Y  P  K  K  P  K  D  E  A  F  R  S  H  Y  K  
ENSG00000      1500 CAGCTCAAAAATCTCTATCCCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTACAAG

ENSG00000       520 P  E  Q  M  G  K  D  G  R  G  Y  V  P  A  T  I  K  M  T  V  
ENSG00000      1560 CCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCCCAGCTACCATCAAGATGACCGTG

ENSG00000       540 E  R  D  Q  P  L  P  T  P  E  L  Q  M  P  T  M  V  P  S  Y  
ENSG00000      1620 GAAAGGGACCAACCACTTCCTACCCCAGAGCTCCAGATGCCTACCATGGTGCCTTCTTAT

ENSG00000       560 D  L  G  M  A  P  D  S  S  M  S  M  Q  L  G  P  D  M  V  P  
ENSG00000      1680 GACCTTGGAATGGCCCCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCC

ENSG00000       580 Q  V  Y  P  P  H  S  H  S  I  P  P  Y  Q  G  L  S  P  E  E  
ENSG00000      1740 CAGGTGTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAGAAGAA

ENSG00000       600 S  V  N  V  L  S  A  F  Q  E  P  H  L  Q  M  P  P  S  L  G  
ENSG00000      1800 TCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATGCCCCCCAGCCTGGGC

ENSG00000       620 Q  M  S  L  P  F  D  Q  P  H  P  Q  G  L  L  P  C  Q  P  Q  
ENSG00000      1860 CAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCAGGGCCTGCTGCCGTGCCAGCCTCAG

ENSG00000       640 E  H  A  V  S  S  P  D  P  L  L  C  S  D  V  T  M  V  E  D  
ENSG00000      1920 GAGCATGCTGTGTCCAGCCCTGACCCCCTGCTCTGCTCAGATGTGACCATGGTGGAAGAC

ENSG00000       660 S  C  L  S  Q  P  V  T  A  F  P  Q  G  T  W  I  G  E  D  I  
ENSG00000      1980 AGCTGCCTGAGCCAGCCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATA

ENSG00000       680 F  P  P  L  L  P  P  T  E  Q  D  L  T  K  L  L  L  E  G  Q  
ENSG00000      2040 TTCCCTCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGGGGCAA

ENSG00000       700 G  E  S  G  G  G  S  L  G  A  Q  P  L  L  Q  P  S  H  Y  G  
ENSG00000      2100 GGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAGCCCTCCCACTATGGG

ENSG00000       720 Q  S  G  I  S  M  S  H  M  D  L  R  A  N  P  S  W    737
ENSG00000      2160 CAATCTGGGATCTCAATGTCCCACATGGACCTAAGGGCCAACCCCAGTTGG 2211
""",
        )
        protein_record = protein_alignment.sequences[5]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 737], [0, 2211]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  E  Q  F  R  H  L  P  M  P  F  H  W  K  Q  E  E  L  K  F  
ENSG00000         0 ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGAACTCAAGTTT

ENSG00000        20 K  T  G  L  R  R  L  Q  H  R  V  G  E  I  H  L  L  R  E  A  
ENSG00000        60 AAGACAGGCTTGCGGAGGCTGCAGCACCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCC

ENSG00000        40 L  Q  K  G  A  E  A  G  Q  V  S  L  H  S  L  I  E  T  P  A  
ENSG00000       120 CTGCAGAAGGGGGCTGAGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCT

ENSG00000        60 N  G  T  G  P  S  E  A  L  A  M  L  L  Q  E  T  T  G  E  L  
ENSG00000       180 AATGGGACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGAGCTA

ENSG00000        80 E  A  A  K  A  L  V  L  K  R  I  Q  I  W  K  R  Q  Q  Q  L  
ENSG00000       240 GAGGCAGCCAAAGCCCTAGTGCTGAAGAGGATCCAGATTTGGAAACGGCAGCAGCAGCTG

ENSG00000       100 A  G  N  G  A  P  F  E  E  S  L  A  P  L  Q  E  R  C  E  S  
ENSG00000       300 GCAGGGAATGGCGCACCGTTTGAGGAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGC

ENSG00000       120 L  V  D  I  Y  S  Q  L  Q  Q  E  V  G  A  A  G  G  E  L  E  
ENSG00000       360 CTGGTGGACATTTATTCCCAGCTACAGCAGGAGGTAGGGGCGGCTGGTGGGGAGCTTGAG

ENSG00000       140 P  K  T  R  A  S  L  T  G  R  L  D  E  V  L  R  T  L  V  T  
ENSG00000       420 CCCAAGACCCGGGCATCGCTGACTGGCCGGCTGGATGAAGTCCTGAGAACCCTCGTCACC

ENSG00000       160 S  C  F  L  V  E  K  Q  P  P  Q  V  L  K  T  Q  T  K  F  Q  
ENSG00000       480 AGTTGCTTCCTGGTGGAGAAGCAGCCCCCCCAGGTACTGAAGACTCAGACCAAGTTCCAG

ENSG00000       180 A  G  V  R  F  L  L  G  L  R  F  L  G  A  P  A  K  P  P  L  
ENSG00000       540 GCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTCCTGGGGGCCCCAGCCAAGCCTCCGCTG

ENSG00000       200 V  R  A  D  M  V  T  E  K  Q  A  R  E  L  S  V  P  Q  G  P  
ENSG00000       600 GTCAGGGCCGACATGGTGACAGAGAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCT

ENSG00000       220 G  A  G  A  E  S  T  G  E  I  I  N  N  T  V  P  L  E  N  S  
ENSG00000       660 GGGGCTGGAGCAGAAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGC

ENSG00000       240 I  P  G  N  C  C  S  A  L  F  K  N  L  L  L  K  K  I  K  R  
ENSG00000       720 ATTCCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGATCAAGCGG

ENSG00000       260 C  E  R  K  G  T  E  S  V  T  E  E  K  C  A  V  L  F  S  A  
ENSG00000       780 TGTGAGCGGAAGGGCACTGAGTCTGTCACAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCC

ENSG00000       280 S  F  T  L  G  P  G  K  L  P  I  Q  L  Q  A  L  S  L  P  L  
ENSG00000       840 AGCTTCACACTTGGCCCCGGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTG

ENSG00000       300 V  V  I  V  H  G  N  Q  D  N  N  A  K  A  T  I  L  W  D  N  
ENSG00000       900 GTGGTCATCGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACAAT

ENSG00000       320 A  F  S  E  M  D  R  V  P  F  V  V  A  E  R  V  P  W  E  K  
ENSG00000       960 GCCTTCTCTGAGATGGACCGCGTGCCCTTTGTGGTGGCTGAGCGGGTGCCCTGGGAGAAG

ENSG00000       340 M  C  E  T  L  N  L  K  F  M  A  E  V  G  T  N  R  G  L  L  
ENSG00000      1020 ATGTGTGAAACTCTGAACCTGAAGTTCATGGCTGAGGTGGGGACCAACCGGGGGCTGCTC

ENSG00000       360 P  E  H  F  L  F  L  A  Q  K  I  F  N  D  N  S  L  S  M  E  
ENSG00000      1080 CCAGAGCACTTCCTCTTCCTGGCCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAG

ENSG00000       380 A  F  Q  H  R  S  V  S  W  S  Q  F  N  K  E  I  L  L  G  R  
ENSG00000      1140 GCCTTCCAGCACCGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGT

ENSG00000       400 G  F  T  F  W  Q  W  F  D  G  V  L  D  L  T  K  R  C  L  R  
ENSG00000      1200 GGCTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCTGTCTCCGG

ENSG00000       420 S  Y  W  S  D  R  L  I  I  G  F  I  S  K  Q  Y  V  T  S  L  
ENSG00000      1260 AGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAACAGTACGTTACTAGCCTT

ENSG00000       440 L  L  N  E  P  D  G  T  F  L  L  R  F  S  D  S  E  I  G  G  
ENSG00000      1320 CTTCTCAATGAGCCCGACGGAACCTTTCTCCTCCGCTTCAGCGACTCAGAGATTGGGGGC

ENSG00000       460 I  T  I  A  H  V  I  R  G  Q  D  G  S  P  Q  I  E  N  I  Q  
ENSG00000      1380 ATCACCATTGCCCATGTCATCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAG

ENSG00000       480 P  F  S  A  K  D  L  S  I  R  S  L  G  D  R  I  R  D  L  A  
ENSG00000      1440 CCATTCTCTGCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGATCTTGCT

ENSG00000       500 Q  L  K  N  L  Y  P  K  K  P  K  D  E  A  F  R  S  H  Y  K  
ENSG00000      1500 CAGCTCAAAAATCTCTATCCCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTACAAG

ENSG00000       520 P  E  Q  M  G  K  D  G  R  G  Y  V  P  A  T  I  K  M  T  V  
ENSG00000      1560 CCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCCCAGCTACCATCAAGATGACCGTG

ENSG00000       540 E  R  D  Q  P  L  P  T  P  E  L  Q  M  P  T  M  V  P  S  Y  
ENSG00000      1620 GAAAGGGACCAACCACTTCCTACCCCAGAGCTCCAGATGCCTACCATGGTGCCTTCTTAT

ENSG00000       560 D  L  G  M  A  P  D  S  S  M  S  M  Q  L  G  P  D  M  V  P  
ENSG00000      1680 GACCTTGGAATGGCCCCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCC

ENSG00000       580 Q  V  Y  P  P  H  S  H  S  I  P  P  Y  Q  G  L  S  P  E  E  
ENSG00000      1740 CAGGTGTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAGAAGAA

ENSG00000       600 S  V  N  V  L  S  A  F  Q  E  P  H  L  Q  M  P  P  S  L  G  
ENSG00000      1800 TCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATGCCCCCCAGCCTGGGC

ENSG00000       620 Q  M  S  L  P  F  D  Q  P  H  P  Q  G  L  L  P  C  Q  P  Q  
ENSG00000      1860 CAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCAGGGCCTGCTGCCGTGCCAGCCTCAG

ENSG00000       640 E  H  A  V  S  S  P  D  P  L  L  C  S  D  V  T  M  V  E  D  
ENSG00000      1920 GAGCATGCTGTGTCCAGCCCTGACCCCCTGCTCTGCTCAGATGTGACCATGGTGGAAGAC

ENSG00000       660 S  C  L  S  Q  P  V  T  A  F  P  Q  G  T  W  I  G  E  D  I  
ENSG00000      1980 AGCTGCCTGAGCCAGCCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATA

ENSG00000       680 F  P  P  L  L  P  P  T  E  Q  D  L  T  K  L  L  L  E  G  Q  
ENSG00000      2040 TTCCCTCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGGGGCAA

ENSG00000       700 G  E  S  G  G  G  S  L  G  A  Q  P  L  L  Q  P  S  H  Y  G  
ENSG00000      2100 GGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAGCCCTCCCACTATGGG

ENSG00000       720 Q  S  G  I  S  M  S  H  M  D  L  R  A  N  P  S  W    737
ENSG00000      2160 CAATCTGGGATCTCAATGTCCCACATGGACCTAAGGGCCAACCCCAGTTGG 2211
""",
        )
        protein_record = protein_alignment.sequences[6]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 737], [0, 2211]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  E  Q  F  R  H  L  P  M  P  F  H  W  K  Q  E  E  L  K  F  
ENSG00000         0 ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGAACTCAAGTTT

ENSG00000        20 K  T  G  L  R  R  L  Q  H  R  V  G  E  I  H  L  L  R  E  A  
ENSG00000        60 AAGACAGGCTTGCGGAGGCTGCAGCACCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCC

ENSG00000        40 L  Q  K  G  A  E  A  G  Q  V  S  L  H  S  L  I  E  T  P  A  
ENSG00000       120 CTGCAGAAGGGGGCTGAGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCT

ENSG00000        60 N  G  T  G  P  S  E  A  L  A  M  L  L  Q  E  T  T  G  E  L  
ENSG00000       180 AATGGGACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGAGCTA

ENSG00000        80 E  A  A  K  A  L  V  L  K  R  I  Q  I  W  K  R  Q  Q  Q  L  
ENSG00000       240 GAGGCAGCCAAAGCCCTAGTGCTGAAGAGGATCCAGATTTGGAAACGGCAGCAGCAGCTG

ENSG00000       100 A  G  N  G  A  P  F  E  E  S  L  A  P  L  Q  E  R  C  E  S  
ENSG00000       300 GCAGGGAATGGCGCACCGTTTGAGGAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGC

ENSG00000       120 L  V  D  I  Y  S  Q  L  Q  Q  E  V  G  A  A  G  G  E  L  E  
ENSG00000       360 CTGGTGGACATTTATTCCCAGCTACAGCAGGAGGTAGGGGCGGCTGGTGGGGAGCTTGAG

ENSG00000       140 P  K  T  R  A  S  L  T  G  R  L  D  E  V  L  R  T  L  V  T  
ENSG00000       420 CCCAAGACCCGGGCATCGCTGACTGGCCGGCTGGATGAAGTCCTGAGAACCCTCGTCACC

ENSG00000       160 S  C  F  L  V  E  K  Q  P  P  Q  V  L  K  T  Q  T  K  F  Q  
ENSG00000       480 AGTTGCTTCCTGGTGGAGAAGCAGCCCCCCCAGGTACTGAAGACTCAGACCAAGTTCCAG

ENSG00000       180 A  G  V  R  F  L  L  G  L  R  F  L  G  A  P  A  K  P  P  L  
ENSG00000       540 GCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTCCTGGGGGCCCCAGCCAAGCCTCCGCTG

ENSG00000       200 V  R  A  D  M  V  T  E  K  Q  A  R  E  L  S  V  P  Q  G  P  
ENSG00000       600 GTCAGGGCCGACATGGTGACAGAGAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCT

ENSG00000       220 G  A  G  A  E  S  T  G  E  I  I  N  N  T  V  P  L  E  N  S  
ENSG00000       660 GGGGCTGGAGCAGAAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGC

ENSG00000       240 I  P  G  N  C  C  S  A  L  F  K  N  L  L  L  K  K  I  K  R  
ENSG00000       720 ATTCCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGATCAAGCGG

ENSG00000       260 C  E  R  K  G  T  E  S  V  T  E  E  K  C  A  V  L  F  S  A  
ENSG00000       780 TGTGAGCGGAAGGGCACTGAGTCTGTCACAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCC

ENSG00000       280 S  F  T  L  G  P  G  K  L  P  I  Q  L  Q  A  L  S  L  P  L  
ENSG00000       840 AGCTTCACACTTGGCCCCGGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTG

ENSG00000       300 V  V  I  V  H  G  N  Q  D  N  N  A  K  A  T  I  L  W  D  N  
ENSG00000       900 GTGGTCATCGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACAAT

ENSG00000       320 A  F  S  E  M  D  R  V  P  F  V  V  A  E  R  V  P  W  E  K  
ENSG00000       960 GCCTTCTCTGAGATGGACCGCGTGCCCTTTGTGGTGGCTGAGCGGGTGCCCTGGGAGAAG

ENSG00000       340 M  C  E  T  L  N  L  K  F  M  A  E  V  G  T  N  R  G  L  L  
ENSG00000      1020 ATGTGTGAAACTCTGAACCTGAAGTTCATGGCTGAGGTGGGGACCAACCGGGGGCTGCTC

ENSG00000       360 P  E  H  F  L  F  L  A  Q  K  I  F  N  D  N  S  L  S  M  E  
ENSG00000      1080 CCAGAGCACTTCCTCTTCCTGGCCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAG

ENSG00000       380 A  F  Q  H  R  S  V  S  W  S  Q  F  N  K  E  I  L  L  G  R  
ENSG00000      1140 GCCTTCCAGCACCGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGT

ENSG00000       400 G  F  T  F  W  Q  W  F  D  G  V  L  D  L  T  K  R  C  L  R  
ENSG00000      1200 GGCTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCTGTCTCCGG

ENSG00000       420 S  Y  W  S  D  R  L  I  I  G  F  I  S  K  Q  Y  V  T  S  L  
ENSG00000      1260 AGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAACAGTACGTTACTAGCCTT

ENSG00000       440 L  L  N  E  P  D  G  T  F  L  L  R  F  S  D  S  E  I  G  G  
ENSG00000      1320 CTTCTCAATGAGCCCGACGGAACCTTTCTCCTCCGCTTCAGCGACTCAGAGATTGGGGGC

ENSG00000       460 I  T  I  A  H  V  I  R  G  Q  D  G  S  P  Q  I  E  N  I  Q  
ENSG00000      1380 ATCACCATTGCCCATGTCATCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAG

ENSG00000       480 P  F  S  A  K  D  L  S  I  R  S  L  G  D  R  I  R  D  L  A  
ENSG00000      1440 CCATTCTCTGCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGATCTTGCT

ENSG00000       500 Q  L  K  N  L  Y  P  K  K  P  K  D  E  A  F  R  S  H  Y  K  
ENSG00000      1500 CAGCTCAAAAATCTCTATCCCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTACAAG

ENSG00000       520 P  E  Q  M  G  K  D  G  R  G  Y  V  P  A  T  I  K  M  T  V  
ENSG00000      1560 CCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCCCAGCTACCATCAAGATGACCGTG

ENSG00000       540 E  R  D  Q  P  L  P  T  P  E  L  Q  M  P  T  M  V  P  S  Y  
ENSG00000      1620 GAAAGGGACCAACCACTTCCTACCCCAGAGCTCCAGATGCCTACCATGGTGCCTTCTTAT

ENSG00000       560 D  L  G  M  A  P  D  S  S  M  S  M  Q  L  G  P  D  M  V  P  
ENSG00000      1680 GACCTTGGAATGGCCCCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCC

ENSG00000       580 Q  V  Y  P  P  H  S  H  S  I  P  P  Y  Q  G  L  S  P  E  E  
ENSG00000      1740 CAGGTGTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAGAAGAA

ENSG00000       600 S  V  N  V  L  S  A  F  Q  E  P  H  L  Q  M  P  P  S  L  G  
ENSG00000      1800 TCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATGCCCCCCAGCCTGGGC

ENSG00000       620 Q  M  S  L  P  F  D  Q  P  H  P  Q  G  L  L  P  C  Q  P  Q  
ENSG00000      1860 CAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCAGGGCCTGCTGCCGTGCCAGCCTCAG

ENSG00000       640 E  H  A  V  S  S  P  D  P  L  L  C  S  D  V  T  M  V  E  D  
ENSG00000      1920 GAGCATGCTGTGTCCAGCCCTGACCCCCTGCTCTGCTCAGATGTGACCATGGTGGAAGAC

ENSG00000       660 S  C  L  S  Q  P  V  T  A  F  P  Q  G  T  W  I  G  E  D  I  
ENSG00000      1980 AGCTGCCTGAGCCAGCCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATA

ENSG00000       680 F  P  P  L  L  P  P  T  E  Q  D  L  T  K  L  L  L  E  G  Q  
ENSG00000      2040 TTCCCTCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGGGGCAA

ENSG00000       700 G  E  S  G  G  G  S  L  G  A  Q  P  L  L  Q  P  S  H  Y  G  
ENSG00000      2100 GGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAGCCCTCCCACTATGGG

ENSG00000       720 Q  S  G  I  S  M  S  H  M  D  L  R  A  N  P  S  W    737
ENSG00000      2160 CAATCTGGGATCTCAATGTCCCACATGGACCTAAGGGCCAACCCCAGTTGG 2211
""",
        )
        protein_record = protein_alignment.sequences[7]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        nucleotide_record = nucleotide_record.upper()
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1021], [0, 3063]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig466         0 E  V  T  Q  S  R  R  K  P  V  R  E  G  R  P  W  E  P  S  Q  
isotig466         0 GAGGTTACTCAGAGTAGGAGGAAGCCGGTCAGAGAGGGCAGACCCTGGGAACCTTCGCAG

isotig466        20 E  L  A  Q  T  P  E  G  G  C  P  R  Q  E  S  R  P  E  G  G  
isotig466        60 GAGTTGGCCCAAACCCCTGAGGGAGGCTGTCCTCGGCAGGAGAGCAGACCTGAGGGAGGT

isotig466        40 W  C  A  G  P  G  C  P  A  L  P  S  H  P  R  A  G  S  P  E  
isotig466       120 TGGTGTGCCGGTCCTGGTTGTCCAGCCCTCCCGTCCCATCCCAGGGCTGGCTCACCAGAA

isotig466        60 K  S  T  G  I  P  G  P  L  G  R  E  Q  R  V  S  S  C  P  G  
isotig466       180 AAAAGTACAGGCATCCCAGGCCCTCTGGGGCGGGAGCAGAGGGTCTCCTCTTGTCCGGGG

isotig466        80 E  D  K  N  K  N  K  K  R  V  C  S  P  H  W  K  S  S  G  A  
isotig466       240 GAAGACAAAAACAAAAACAAAAAACGAGTGTGTAGCCCTCACTGGAAGTCTTCTGGTGCT

isotig466       100 L  G  P  F  A  L  G  S  L  A  S  G  H  G  G  R  A  P  P  G  
isotig466       300 CTGGGGCCGTTTGCACTTGGGAGCCTGGCTTCTGGGCATGGTGGCCGGGCTCCGCCGGGG

isotig466       120 A  S  A  L  G  K  A  F  L  E  Q  S  G  W  E  P  T  E  V  P  
isotig466       360 GCTTCAGCCCTCGGCAAAGCGTTTCTAGAACAGAGTGGGTGGGAGCCGACTGAAGTCCCA

isotig466       140 E  P  R  T  L  T  H  R  K  A  S  S  G  W  R  N  A  N  P  P  
isotig466       420 GAACCGCGAACACTGACGCACAGGAAAGCCTCGAGTGGGTGGAGAAATGCAAATCCCCCA

isotig466       160 V  A  W  P  Q  P  L  R  T  L  T  A  D  S  D  F  G  L  E  R  
isotig466       480 GTGGCATGGCCTCAGCCGCTGCGGACCCTGACGGCCGATTCTGACTTCGGACTTGAGCGG

isotig466       180 D  Q  H  P  D  P  G  R  A  S  M  S  Q  W  N  Q  V  Q  Q  L  
isotig466       540 GACCAGCACCCGGACCCAGGGCGAGCCAGCATGTCTCAGTGGAATCAAGTCCAACAGTTA

isotig466       200 E  I  K  F  L  E  Q  V  D  Q  F  Y  D  D  N  F  P  M  E  I  
isotig466       600 GAAATCAAGTTTTTGGAGCAAGTTGATCAGTTCTATGATGACAACTTTCCCATGGAAATC

isotig466       220 R  H  L  L  A  Q  W  I  E  H  Q  D  W  E  V  A  S  N  N  E  
isotig466       660 CGACATCTGCTGGCCCAGTGGATTGAGCATCAAGACTGGGAGGTGGCCTCTAACAATGAA

isotig466       240 T  M  A  T  I  L  L  Q  N  L  L  I  Q  L  D  E  Q  L  G  R  
isotig466       720 ACTATGGCAACAATTCTTCTTCAAAACTTATTAATACAATTGGATGAACAGTTAGGTCGT

isotig466       260 V  S  K  E  K  N  L  L  L  I  H  N  L  K  R  I  R  K  V  L  
isotig466       780 GTTTCCAAAGAGAAAAACCTGCTATTGATCCACAATCTAAAGAGAATTAGAAAAGTACTT

isotig466       280 Q  G  K  F  H  G  N  P  M  H  V  A  V  V  I  S  N  C  L  R  
isotig466       840 CAGGGGAAGTTTCATGGAAATCCAATGCATGTAGCCGTGGTAATCTCAAATTGTTTAAGG

isotig466       300 E  E  R  R  I  L  A  A  A  N  M  P  I  Q  G  P  L  E  K  S  
isotig466       900 GAAGAGAGGAGAATACTGGCTGCAGCGAACATGCCTATCCAGGGACCTCTGGAGAAATCC

isotig466       320 L  Q  S  S  S  V  S  E  R  Q  R  N  V  E  H  K  V  A  A  I  
isotig466       960 TTACAAAGTTCGTCGGTTTCAGAAAGACAGAGAAATGTGGAACACAAAGTGGCTGCCATT

isotig466       340 K  N  S  V  Q  M  T  E  Q  D  T  K  Y  L  E  D  L  Q  D  E  
isotig466      1020 AAAAACAGTGTGCAGATGACAGAACAAGACACCAAATACTTGGAAGATCTGCAAGATGAA

isotig466       360 F  D  Y  R  Y  K  T  I  Q  T  M  D  Q  G  D  K  N  S  I  L  
isotig466      1080 TTTGACTACAGGTATAAAACAATTCAGACAATGGACCAGGGTGACAAGAATAGCATCCTA

isotig466       380 M  N  Q  E  V  L  T  L  Q  E  M  L  N  S  L  D  F  K  R  K  
isotig466      1140 ATGAACCAGGAGGTTTTGACACTCCAAGAAATGCTTAATAGCCTGGACTTCAAGAGAAAG

isotig466       400 E  A  L  T  K  M  T  Q  I  V  N  E  S  D  L  L  M  S  S  M  
isotig466      1200 GAAGCACTCACTAAGATGACACAGATAGTGAACGAGTCGGACCTGCTGATGAGCAGCATG

isotig466       420 L  I  E  E  L  Q  D  W  K  R  R  Q  Q  I  A  C  I  G  G  P  
isotig466      1260 CTCATAGAAGAGCTGCAGGACTGGAAGAGGAGGCAGCAGATCGCCTGCATCGGTGGCCCA

isotig466       440 L  H  N  G  L  D  Q  L  Q  N  C  F  T  L  L  A  E  S  L  F  
isotig466      1320 CTCCACAACGGGCTGGACCAGCTTCAGAACTGCTTTACCCTGTTGGCAGAAAGTCTTTTC

isotig466       460 Q  L  R  R  Q  L  E  K  L  E  E  Q  S  S  K  M  T  Y  E  G  
isotig466      1380 CAACTCAGACGACAGCTGGAGAAATTAGAGGAGCAGTCTTCCAAGATGACTTACGAAGGA

isotig466       480 D  P  I  P  T  Q  R  A  H  L  L  E  R  A  T  F  L  I  Y  N  
isotig466      1440 GACCCCATCCCCACGCAGAGAGCACACCTGCTGGAGAGAGCCACCTTCCTGATCTACAAC

isotig466       500 L  F  K  N  S  F  V  V  E  R  Q  P  C  M  P  T  H  P  Q  R  
isotig466      1500 CTTTTCAAGAACTCATTTGTGGTTGAGCGACAGCCCTGCATGCCAACACACCCTCAGAGG

isotig466       520 P  L  V  L  K  T  L  I  Q  F  T  A  K  L  R  L  L  I  K  L  
isotig466      1560 CCGCTGGTACTCAAAACCCTCATTCAGTTCACCGCGAAACTGAGACTACTAATAAAATTG

isotig466       540 P  E  L  N  Y  Q  V  K  V  K  A  S  I  D  K  N  V  S  T  L  
isotig466      1620 CCGGAACTCAACTATCAGGTGAAAGTAAAGGCATCGATCGACAAGAATGTTTCAACGCTA

isotig466       560 S  N  R  R  F  V  L  C  G  T  Q  V  K  A  M  S  I  E  E  S  
isotig466      1680 AGCAATAGAAGATTTGTGCTTTGTGGAACTCAAGTCAAAGCCATGTCCATCGAGGAATCC

isotig466       580 S  N  G  S  L  S  V  E  F  R  H  L  Q  P  K  E  M  K  S  S  
isotig466      1740 TCCAATGGGAGCCTCTCAGTAGAATTTAGACATTTGCAACCGAAGGAAATGAAATCCAGT

isotig466       600 A  G  S  K  G  N  E  G  C  H  M  V  T  E  E  L  H  S  I  A  
isotig466      1800 GCCGGAAGTAAAGGAAATGAGGGCTGCCACATGGTGACGGAAGAGCTGCATTCCATAGCC

isotig466       620 F  E  T  Q  I  C  L  Y  G  L  T  I  D  L  E  T  S  S  L  P  
isotig466      1860 TTTGAGACCCAGATCTGCCTCTATGGCCTCACCATCGACTTGGAGACAAGCTCATTACCT

isotig466       640 V  V  M  I  S  N  V  S  Q  L  P  N  A  W  A  S  I  I  W  Y  
isotig466      1920 GTGGTGATGATTTCTAATGTCAGCCAACTGCCTAATGCTTGGGCATCCATCATTTGGTAC

isotig466       660 N  V  S  T  N  D  C  Q  N  L  V  F  F  N  N  P  P  P  V  T  
isotig466      1980 AATGTGTCAACCAACGATTGCCAGAACTTGGTTTTCTTTAATAATCCTCCGCCTGTCACT

isotig466       680 L  S  Q  L  L  E  V  M  S  W  Q  F  S  S  Y  V  G  R  G  L  
isotig466      2040 TTGAGTCAACTCCTGGAAGTGATGAGCTGGCAGTTTTCATCCTATGTTGGTCGTGGCCTT

isotig466       700 N  S  D  Q  L  N  M  L  A  E  K  L  T  V  Q  S  N  Y  S  D  
isotig466      2100 AATTCAGACCAGCTCAACATGCTGGCAGAGAAGCTCACAGTTCAGTCTAACTACAGCGAT

isotig466       720 G  H  L  T  W  A  K  F  C  K  E  H  L  P  G  K  P  F  T  F  
isotig466      2160 GGTCACCTCACCTGGGCCAAGTTCTGCAAGGAACACTTGCCTGGCAAACCATTTACCTTC

isotig466       740 W  T  W  L  E  A  I  L  D  L  I  K  K  H  I  L  P  L  W  I  
isotig466      2220 TGGACCTGGCTTGAAGCAATATTGGACCTAATTAAAAAACACATTCTTCCCCTCTGGATT

isotig466       760 D  G  Y  I  M  G  F  V  S  K  E  K  E  R  F  L  L  K  D  K  
isotig466      2280 GATGGGTACATCATGGGCTTCGTGAGCAAAGAGAAGGAGAGGTTTCTGCTCAAGGATAAA

isotig466       780 M  P  G  T  F  L  L  R  F  S  E  S  H  L  G  G  I  T  F  T  
isotig466      2340 ATGCCCGGGACATTTTTGTTACGATTCAGTGAGAGCCATCTCGGAGGGATCACCTTCACC

isotig466       800 W  V  D  H  S  E  N  G  E  V  R  F  H  S  V  E  P  Y  N  K  
isotig466      2400 TGGGTGGACCACTCTGAAAACGGAGAAGTGAGATTCCACTCCGTAGAACCCTACAACAAA

isotig466       820 G  R  L  S  A  L  P  F  A  D  I  L  R  D  Y  K  V  I  M  A  
isotig466      2460 GGGCGTCTGTCGGCCCTGCCATTTGCTGACATCCTGCGGGACTACAAGGTCATCATGGCT

isotig466       840 E  N  I  P  E  N  P  L  K  Y  L  Y  P  D  I  P  K  D  K  A  
isotig466      2520 GAGAACATTCCCGAGAACCCTCTCAAGTACCTCTACCCCGACATCCCCAAAGACAAAGCC

isotig466       860 F  G  K  H  Y  S  S  Q  P  C  E  V  S  R  P  T  E  R  G  D  
isotig466      2580 TTCGGTAAACACTACAGCTCCCAGCCTTGCGAAGTTTCAAGGCCAACAGAACGGGGAGAC

isotig466       880 K  G  Y  V  P  S  V  F  I  P  I  S  T  I  R  S  D  A  M  E  
isotig466      2640 AAAGGTTATGTTCCTTCAGTTTTTATCCCTATTTCAACAATCCGCAGCGACGCCATGGAG

isotig466       900 P  Q  S  P  S  D  L  L  P  M  S  P  S  V  Y  A  V  L  R  E  
isotig466      2700 CCGCAGTCTCCTTCAGACCTTCTCCCCATGTCTCCGAGTGTATACGCTGTGCTGAGAGAA

isotig466       920 N  L  S  P  T  T  I  E  T  A  M  K  S  P  Y  S  E  R  Y  K  
isotig466      2760 AACCTGAGCCCTACCACAATTGAAACAGCAATGAAGTCTCCATATTCTGAGCGGTACAAA

isotig466       940 A  T  L  Q  G  R  E  Q  M  K  T  E  T  A  L  C  Q  S  P  Q  
isotig466      2820 GCGACTCTTCAAGGAAGAGAGCAGATGAAAACGGAGACTGCTCTTTGCCAAAGTCCACAA

isotig466       960 F  I  S  S  A  L  I  L  V  S  R  K  W  H  K  S  E  A  F  L  
isotig466      2880 TTCATTTCTTCAGCTTTGATACTGGTTTCTAGAAAATGGCACAAATCCGAAGCTTTCCTC

isotig466       980 S  L  G  D  I  P  Q  L  G  V  L  L  K  C  K  P  K  L  Q  I  
isotig466      2940 TCACTAGGTGACATTCCCCAACTGGGAGTGCTGCTGAAATGCAAACCAAAGCTTCAGATA

isotig466      1000 N  T  Q  E  K  T  A  S  R  N  L  C  S  Q  Y  N  R  R  L  L  
isotig466      3000 AACACGCAGGAAAAGACAGCTTCGAGAAACCTATGTTCGCAATATAACAGAAGGCTGCTT

isotig466      1020 C   1021
isotig466      3060 TGC 3063
""",
        )
        protein_record = protein_alignment.sequences[8]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        nucleotide_record = nucleotide_record.upper()
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 806], [0, 2418]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig125         0 A  R  R  G  Q  A  A  L  G  S  P  A  A  R  T  W  S  Q  R  S  
isotig125         0 GCTAGGAGAGGCCAGGCGGCCCTCGGGAGCCCAGCTGCTCGCACCTGGAGCCAGCGCAGC

isotig125        20 P  A  S  R  A  S  A  R  E  T  V  T  P  P  D  C  G  R  M  A  
isotig125        60 CCGGCCAGTCGGGCCTCAGCCCGGGAGACAGTTACGCCCCCTGATTGCGGCAGGATGGCC

isotig125        40 Q  W  N  Q  L  Q  Q  L  D  T  R  Y  L  E  Q  L  H  Q  L  Y  
isotig125       120 CAGTGGAACCAGCTGCAGCAGCTGGACACTCGGTACCTGGAGCAGCTGCACCAGCTGTAC

isotig125        60 S  D  S  F  P  M  E  L  R  Q  F  L  A  P  W  I  E  S  Q  D  
isotig125       180 AGCGACAGCTTCCCCATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGAT

isotig125        80 W  A  Y  A  A  S  K  E  S  H  A  T  L  V  F  H  N  L  L  G  
isotig125       240 TGGGCATATGCAGCCAGCAAAGAGTCACATGCCACACTGGTGTTTCATAATCTCTTGGGT

isotig125       100 E  I  D  Q  Q  Y  S  R  F  L  Q  E  S  N  V  L  Y  Q  H  N  
isotig125       300 GAGATTGACCAGCAGTACAGCCGATTCCTGCAAGAGTCCAACGTCCTCTATCAGCACAAC

isotig125       120 L  R  R  I  K  Q  F  L  Q  S  R  Y  L  E  K  P  M  E  I  A  
isotig125       360 CTTCGGAGGATCAAGCAGTTCCTACAGAGCAGGTATCTTGAGAAGCCGATGGAAATCGCC

isotig125       140 R  I  V  A  R  C  L  W  E  E  S  R  L  L  Q  T  A  A  T  A  
isotig125       420 CGCATCGTGGCCCGATGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACTGCA

isotig125       160 A  Q  Q  G  G  Q  A  N  H  P  T  A  A  V  V  T  E  K  Q  Q  
isotig125       480 GCCCAGCAAGGAGGCCAGGCCAACCACCCAACAGCTGCTGTGGTGACGGAGAAACAGCAG

isotig125       180 M  L  E  Q  H  L  Q  D  V  R  K  R  V  Q  D  L  E  Q  K  M  
isotig125       540 ATGCTGGAGCAGCATCTTCAGGATGTCCGGAAACGTGTGCAGGATCTAGAACAGAAAATG

isotig125       200 K  V  V  E  N  L  Q  D  D  F  D  F  N  Y  K  T  L  K  S  Q  
isotig125       600 AAAGTGGTAGAGAATCTCCAGGATGACTTTGATTTCAACTATAAAACCCTCAAGAGTCAA

isotig125       220 G  D  M  Q  D  L  N  G  N  N  Q  S  V  T  R  Q  K  M  Q  Q  
isotig125       660 GGAGACATGCAGGATCTGAATGGAAACAACCAGTCTGTGACCAGGCAGAAGATGCAGCAG

isotig125       240 L  E  Q  M  L  T  A  L  D  Q  M  R  R  S  I  V  S  E  L  A  
isotig125       720 CTGGAACAGATGCTCACGGCGCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCG

isotig125       260 G  L  L  S  A  M  E  Y  V  Q  K  T  L  T  D  E  E  L  A  D  
isotig125       780 GGGCTTTTGTCGGCAATGGAGTACGTGCAGAAAACACTCACAGACGAGGAGCTGGCTGAC

isotig125       280 W  K  R  R  Q  Q  I  A  C  I  G  G  P  P  N  I  C  L  D  R  
isotig125       840 TGGAAGAGGCGGCAGCAGATCGCGTGCATTGGAGGCCCTCCCAACATCTGCCTGGATCGC

isotig125       300 L  E  N  W  I  T  S  L  A  E  S  Q  L  Q  T  R  Q  Q  I  K  
isotig125       900 CTGGAAAACTGGATAACTTCGTTAGCAGAATCTCAACTTCAGACCCGCCAACAAATTAAG

isotig125       320 K  L  E  E  L  Q  Q  K  V  S  Y  K  G  D  P  I  V  Q  H  R  
isotig125       960 AAACTGGAGGAGCTACAGCAGAAGGTGTCCTACAAGGGGGACCCCATTGTGCAGCACCGG

isotig125       340 P  M  L  E  E  R  I  V  E  L  F  R  N  L  M  K  S  A  F  V  
isotig125      1020 CCGATGCTGGAGGAGCGGATCGTGGAGCTGTTCAGAAACTTGATGAAGAGTGCCTTCGTG

isotig125       360 V  E  R  Q  P  C  M  P  M  H  P  D  R  P  L  V  I  K  T  G  
isotig125      1080 GTGGAGCGACAGCCCTGCATGCCGATGCACCCCGACCGGCCCTTGGTCATCAAGACTGGT

isotig125       380 V  Q  F  T  T  K  V  R  L  L  V  K  F  P  E  L  N  Y  Q  L  
isotig125      1140 GTCCAGTTCACTACTAAAGTCAGGTTGTTGGTCAAGTTTCCCGAGTTGAATTATCAGCTT

isotig125       400 K  I  K  V  C  I  D  K  D  S  G  D  V  A  A  L  R  G  S  R  
isotig125      1200 AAAATTAAAGTGTGCATTGACAAAGATTCTGGGGACGTTGCTGCTCTCAGAGGATCTCGG

isotig125       420 K  F  N  I  L  G  T  N  T  K  V  M  N  M  E  E  S  N  N  G  
isotig125      1260 AAATTTAACATTCTGGGCACAAACACGAAGGTGATGAACATGGAAGAATCCAACAACGGC

isotig125       440 S  L  S  A  E  F  K  H  L  T  L  R  E  Q  R  C  G  N  G  G  
isotig125      1320 AGCCTGTCTGCGGAGTTCAAGCACTTGACCCTGAGGGAGCAGAGATGTGGGAATGGAGGC

isotig125       460 R  A  N  C  D  A  S  L  I  V  T  E  E  L  H  L  I  T  F  E  
isotig125      1380 CGTGCCAATTGTGATGCCTCCTTGATTGTGACCGAGGAGCTGCATCTGATCACCTTCGAG

isotig125       480 T  E  V  Y  H  Q  G  L  K  I  D  L  E  T  H  S  L  P  V  V  
isotig125      1440 ACTGAGGTGTACCACCAAGGCCTCAAGATTGACCTGGAGACCCATTCTTTGCCAGTTGTG

isotig125       500 V  I  S  N  I  C  Q  M  P  N  A  W  A  S  I  L  W  Y  N  M  
isotig125      1500 GTGATCTCCAACATCTGTCAGATGCCAAATGCCTGGGCATCCATCCTGTGGTATAACATG

isotig125       520 L  T  N  N  P  K  N  V  N  F  F  T  K  P  P  I  G  T  W  D  
isotig125      1560 CTGACCAACAACCCCAAGAACGTGAACTTCTTCACCAAGCCACCAATCGGAACCTGGGAC

isotig125       540 Q  V  A  E  V  L  S  W  Q  F  S  S  T  T  K  R  G  L  S  I  
isotig125      1620 CAGGTGGCCGAGGTGCTCAGCTGGCAGTTCTCATCCACCACAAAGCGAGGGCTGAGCATC

isotig125       560 E  Q  L  T  T  L  A  E  K  L  L  G  P  G  V  N  Y  S  G  C  
isotig125      1680 GAGCAGCTGACTACGCTGGCCGAGAAGCTCCTAGGACCTGGTGTCAACTACTCCGGGTGT

isotig125       580 Q  I  T  W  A  K  F  C  K  E  N  M  A  G  K  G  F  S  F  W  
isotig125      1740 CAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCCTTCTGG

isotig125       600 V  W  L  D  N  I  I  D  L  V  K  K  Y  I  L  A  L  W  N  E  
isotig125      1800 GTGTGGCTAGACAATATCATTGACCTTGTGAAAAAGTATATCTTGGCCCTCTGGAATGAA

isotig125       620 G  Y  I  M  G  F  I  S  K  E  R  E  R  A  I  L  S  T  K  P  
isotig125      1860 GGGTACATCATGGGCTTCATTAGCAAGGAGCGGGAGCGGGCGATCCTGAGCACGAAACCC

isotig125       640 P  G  T  F  L  L  R  F  S  E  S  S  K  E  G  G  V  T  F  T  
isotig125      1920 CCGGGCACCTTCCTGCTGAGATTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACT

isotig125       660 W  V  E  K  D  I  S  G  K  T  Q  I  Q  S  V  E  P  Y  T  K  
isotig125      1980 TGGGTGGAAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCGTACACCAAG

isotig125       680 Q  Q  L  N  N  M  S  F  A  E  I  I  M  G  Y  K  I  M  D  A  
isotig125      2040 CAGCAGCTGAACAACATGTCCTTTGCTGAAATCATCATGGGCTACAAGATCATGGATGCC

isotig125       700 T  N  I  L  V  S  P  L  V  Y  L  Y  P  D  I  P  K  E  E  A  
isotig125      2100 ACCAACATCCTGGTGTCCCCATTGGTCTACCTCTACCCTGACATTCCCAAGGAGGAGGCG

isotig125       720 F  G  K  Y  C  R  P  E  S  Q  E  H  P  E  A  D  P  G  S  A  
isotig125      2160 TTCGGGAAGTACTGTCGACCAGAGAGCCAGGAGCATCCTGAAGCTGACCCCGGTAGTGCC

isotig125       740 A  P  Y  L  K  T  K  F  I  C  V  T  P  T  T  C  S  N  T  I  
isotig125      2220 GCCCCTTACCTGAAGACCAAGTTCATCTGTGTGACACCAACGACCTGCAGCAATACCATT

isotig125       760 D  L  P  M  S  P  P  H  F  R  F  I  D  A  V  W  K  R  R  C  
isotig125      2280 GACCTGCCGATGTCCCCCCCGCACTTTAGATTCATTGATGCAGTTTGGAAACGGAGGTGC

isotig125       780 A  L  G  R  R  A  V  V  T  H  V  H  G  S  D  F  G  V  R  Y  
isotig125      2340 GCCCTCGGCAGGAGGGCAGTTGTCACTCACGTTCATGGATCTGACTTCGGAGTGCGCTAC

isotig125       800 L  P  H  V  R  S    806
isotig125      2400 CTCCCCCATGTGAGGAGC 2418
""",
        )
        protein_record = protein_alignment.sequences[9]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        nucleotide_record = nucleotide_record.upper()
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 796], [0, 2388]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig125         0 A  R  R  G  Q  A  A  L  G  S  P  A  A  R  T  W  S  Q  R  S  
isotig125         0 GCTAGGAGAGGCCAGGCGGCCCTCGGGAGCCCAGCTGCTCGCACCTGGAGCCAGCGCAGC

isotig125        20 P  A  S  R  A  S  A  R  E  T  V  T  P  P  D  C  G  R  M  A  
isotig125        60 CCGGCCAGTCGGGCCTCAGCCCGGGAGACAGTTACGCCCCCTGATTGCGGCAGGATGGCC

isotig125        40 Q  W  N  Q  L  Q  Q  L  D  T  R  Y  L  E  Q  L  H  Q  L  Y  
isotig125       120 CAGTGGAACCAGCTGCAGCAGCTGGACACTCGGTACCTGGAGCAGCTGCACCAGCTGTAC

isotig125        60 S  D  S  F  P  M  E  L  R  Q  F  L  A  P  W  I  E  S  Q  D  
isotig125       180 AGCGACAGCTTCCCCATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGAT

isotig125        80 W  A  Y  A  A  S  K  E  S  H  A  T  L  V  F  H  N  L  L  G  
isotig125       240 TGGGCATATGCAGCCAGCAAAGAGTCACATGCCACACTGGTGTTTCATAATCTCTTGGGT

isotig125       100 E  I  D  Q  Q  Y  S  R  F  L  Q  E  S  N  V  L  Y  Q  H  N  
isotig125       300 GAGATTGACCAGCAGTACAGCCGATTCCTGCAAGAGTCCAACGTCCTCTATCAGCACAAC

isotig125       120 L  R  R  I  K  Q  F  L  Q  S  R  Y  L  E  K  P  M  E  I  A  
isotig125       360 CTTCGGAGGATCAAGCAGTTCCTACAGAGCAGGTATCTTGAGAAGCCGATGGAAATCGCC

isotig125       140 R  I  V  A  R  C  L  W  E  E  S  R  L  L  Q  T  A  A  T  A  
isotig125       420 CGCATCGTGGCCCGATGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACTGCA

isotig125       160 A  Q  Q  G  G  Q  A  N  H  P  T  A  A  V  V  T  E  K  Q  Q  
isotig125       480 GCCCAGCAAGGAGGCCAGGCCAACCACCCAACAGCTGCTGTGGTGACGGAGAAACAGCAG

isotig125       180 M  L  E  Q  H  L  Q  D  V  R  K  R  V  Q  D  L  E  Q  K  M  
isotig125       540 ATGCTGGAGCAGCATCTTCAGGATGTCCGGAAACGTGTGCAGGATCTAGAACAGAAAATG

isotig125       200 K  V  V  E  N  L  Q  D  D  F  D  F  N  Y  K  T  L  K  S  Q  
isotig125       600 AAAGTGGTAGAGAATCTCCAGGATGACTTTGATTTCAACTATAAAACCCTCAAGAGTCAA

isotig125       220 G  D  M  Q  D  L  N  G  N  N  Q  S  V  T  R  Q  K  M  Q  Q  
isotig125       660 GGAGACATGCAGGATCTGAATGGAAACAACCAGTCTGTGACCAGGCAGAAGATGCAGCAG

isotig125       240 L  E  Q  M  L  T  A  L  D  Q  M  R  R  S  I  V  S  E  L  A  
isotig125       720 CTGGAACAGATGCTCACGGCGCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCG

isotig125       260 G  L  L  S  A  M  E  Y  V  Q  K  T  L  T  D  E  E  L  A  D  
isotig125       780 GGGCTTTTGTCGGCAATGGAGTACGTGCAGAAAACACTCACAGACGAGGAGCTGGCTGAC

isotig125       280 W  K  R  R  Q  Q  I  A  C  I  G  G  P  P  N  I  C  L  D  R  
isotig125       840 TGGAAGAGGCGGCAGCAGATCGCGTGCATTGGAGGCCCTCCCAACATCTGCCTGGATCGC

isotig125       300 L  E  N  W  I  T  S  L  A  E  S  Q  L  Q  T  R  Q  Q  I  K  
isotig125       900 CTGGAAAACTGGATAACTTCGTTAGCAGAATCTCAACTTCAGACCCGCCAACAAATTAAG

isotig125       320 K  L  E  E  L  Q  Q  K  V  S  Y  K  G  D  P  I  V  Q  H  R  
isotig125       960 AAACTGGAGGAGCTACAGCAGAAGGTGTCCTACAAGGGGGACCCCATTGTGCAGCACCGG

isotig125       340 P  M  L  E  E  R  I  V  E  L  F  R  N  L  M  K  S  A  F  V  
isotig125      1020 CCGATGCTGGAGGAGCGGATCGTGGAGCTGTTCAGAAACTTGATGAAGAGTGCCTTCGTG

isotig125       360 V  E  R  Q  P  C  M  P  M  H  P  D  R  P  L  V  I  K  T  G  
isotig125      1080 GTGGAGCGACAGCCCTGCATGCCGATGCACCCCGACCGGCCCTTGGTCATCAAGACTGGT

isotig125       380 V  Q  F  T  T  K  V  R  L  L  V  K  F  P  E  L  N  Y  Q  L  
isotig125      1140 GTCCAGTTCACTACTAAAGTCAGGTTGTTGGTCAAGTTTCCCGAGTTGAATTATCAGCTT

isotig125       400 K  I  K  V  C  I  D  K  D  S  G  D  V  A  A  L  R  G  S  R  
isotig125      1200 AAAATTAAAGTGTGCATTGACAAAGATTCTGGGGACGTTGCTGCTCTCAGAGGATCTCGG

isotig125       420 K  F  N  I  L  G  T  N  T  K  V  M  N  M  E  E  S  N  N  G  
isotig125      1260 AAATTTAACATTCTGGGCACAAACACGAAGGTGATGAACATGGAAGAATCCAACAACGGC

isotig125       440 S  L  S  A  E  F  K  H  L  T  L  R  E  Q  R  C  G  N  G  G  
isotig125      1320 AGCCTGTCTGCGGAGTTCAAGCACTTGACCCTGAGGGAGCAGAGATGTGGGAATGGAGGC

isotig125       460 R  A  N  C  D  A  S  L  I  V  T  E  E  L  H  L  I  T  F  E  
isotig125      1380 CGTGCCAATTGTGATGCCTCCTTGATTGTGACCGAGGAGCTGCATCTGATCACCTTCGAG

isotig125       480 T  E  V  Y  H  Q  G  L  K  I  D  L  E  T  H  S  L  P  V  V  
isotig125      1440 ACTGAGGTGTACCACCAAGGCCTCAAGATTGACCTGGAGACCCATTCTTTGCCAGTTGTG

isotig125       500 V  I  S  N  I  C  Q  M  P  N  A  W  A  S  I  L  W  Y  N  M  
isotig125      1500 GTGATCTCCAACATCTGTCAGATGCCAAATGCCTGGGCATCCATCCTGTGGTATAACATG

isotig125       520 L  T  N  N  P  K  N  V  N  F  F  T  K  P  P  I  G  T  W  D  
isotig125      1560 CTGACCAACAACCCCAAGAACGTGAACTTCTTCACCAAGCCACCAATCGGAACCTGGGAC

isotig125       540 Q  V  A  E  V  L  S  W  Q  F  S  S  T  T  K  R  G  L  S  I  
isotig125      1620 CAGGTGGCCGAGGTGCTCAGCTGGCAGTTCTCATCCACCACAAAGCGAGGGCTGAGCATC

isotig125       560 E  Q  L  T  T  L  A  E  K  L  L  G  P  G  V  N  Y  S  G  C  
isotig125      1680 GAGCAGCTGACTACGCTGGCCGAGAAGCTCCTAGGACCTGGTGTCAACTACTCCGGGTGT

isotig125       580 Q  I  T  W  A  K  F  C  K  E  N  M  A  G  K  G  F  S  F  W  
isotig125      1740 CAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCCTTCTGG

isotig125       600 V  W  L  D  N  I  I  D  L  V  K  K  Y  I  L  A  L  W  N  E  
isotig125      1800 GTGTGGCTAGACAATATCATTGACCTTGTGAAAAAGTATATCTTGGCCCTCTGGAATGAA

isotig125       620 G  Y  I  M  G  F  I  S  K  E  R  E  R  A  I  L  S  T  K  P  
isotig125      1860 GGGTACATCATGGGCTTCATTAGCAAGGAGCGGGAGCGGGCGATCCTGAGCACGAAACCC

isotig125       640 P  G  T  F  L  L  R  F  S  E  S  S  K  E  G  G  V  T  F  T  
isotig125      1920 CCGGGCACCTTCCTGCTGAGATTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACT

isotig125       660 W  V  E  K  D  I  S  G  K  T  Q  I  Q  S  V  E  P  Y  T  K  
isotig125      1980 TGGGTGGAAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCGTACACCAAG

isotig125       680 Q  Q  L  N  N  M  S  F  A  E  I  I  M  G  Y  K  I  M  D  A  
isotig125      2040 CAGCAGCTGAACAACATGTCCTTTGCTGAAATCATCATGGGCTACAAGATCATGGATGCC

isotig125       700 T  N  I  L  V  S  P  L  V  Y  L  Y  P  D  I  P  K  E  E  A  
isotig125      2100 ACCAACATCCTGGTGTCCCCATTGGTCTACCTCTACCCTGACATTCCCAAGGAGGAGGCG

isotig125       720 F  G  K  Y  C  R  P  E  S  Q  E  H  P  E  A  D  P  G  S  C  
isotig125      2160 TTCGGGAAGTACTGTCGACCAGAGAGCCAGGAGCATCCTGAAGCTGACCCCGGTAGTTGT

isotig125       740 F  S  M  V  L  V  S  L  L  G  K  G  G  Q  C  R  S  L  E  E  
isotig125      2220 TTTTCCATGGTTCTGGTTTCGCTGTTAGGGAAAGGGGGACAGTGCAGGTCCTTGGAGGAG

isotig125       760 R  Q  G  H  D  R  V  S  G  G  E  S  C  Y  G  R  A  V  Y  W  
isotig125      2280 AGACAAGGACATGACCGGGTGTCTGGTGGTGAGTCCTGCTATGGAAGAGCTGTTTATTGG

isotig125       780 V  L  Q  G  D  R  D  S  R  E  D  Q  N  Q  A  S    796
isotig125      2340 GTACTTCAGGGTGACCGGGATTCAAGAGAAGACCAGAATCAGGCCTCA 2388
""",
        )
        alignment = protein_alignment.mapall(codon_alignments)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
                np.array([[   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,   36,   36,   63,   63,
                            213,  222,  240,  240,  330,  330,  330,  405,
                            405,  438,  450,  579,  579,  729,  729,  837,
                            837,  897,  912,  975,  987, 1020, 1026, 1074,
                           1077, 1107, 1107, 1116, 1119, 1182, 1188, 1302,
                           1302, 1392, 1398, 1455, 1458, 1470, 1695, 1695,
                           1824, 1824, 1887, 1887, 1896, 1902, 1956, 1980,
                           2022, 2025, 2043, 2046, 2061, 2106, 2187, 2220,
                           2232, 2253, 2271, 2331, 2334, 2352, 2364, 2373,
                           2382, 2424, 2463, 2484, 2517, 2541],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,   36,   36,   63,   63,
                            213,  222,  240,  240,  330,  330,  330,  405,
                            405,  438,  450,  579,  579,  729,  729,  837,
                            837,  897,  912,  975,  987, 1020, 1026, 1074,
                           1077, 1107, 1107, 1116, 1119, 1182, 1188, 1302,
                           1302, 1392, 1398, 1455, 1458, 1470, 1695, 1695,
                           1824, 1824, 1887, 1887, 1896, 1902, 1956, 1980,
                           2022, 2025, 2043, 2046, 2061, 2106, 2187, 2220,
                           2232, 2253, 2271, 2331, 2334, 2352, 2364, 2373,
                           2382, 2424, 2463, 2484, 2517, 2541],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,   36,   36,   63,   63,
                            213,  222,  240,  240,  330,  330,  330,  405,
                            405,  438,  450,  579,  579,  729,  729,  837,
                            837,  897,  912,  975,  987, 1020, 1026, 1074,
                           1077, 1107, 1107, 1116, 1119, 1182, 1188, 1302,
                           1302, 1392, 1398, 1455, 1458, 1470, 1695, 1695,
                           1824, 1824, 1887, 1887, 1896, 1902, 1956, 1980,
                           2022, 2025, 2043, 2046, 2061, 2106, 2187, 2220,
                           2232, 2253, 2271, 2331, 2334, 2352, 2364, 2373,
                           2382, 2424, 2463, 2484, 2517, 2541],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,   36,   36,   63,   63,
                            213,  222,  240,  240,  330,  330,  330,  405,
                            405,  438,  450,  579,  579,  729,  729,  837,
                            837,  897,  912,  975,  987, 1020, 1026, 1074,
                           1077, 1107, 1107, 1116, 1119, 1182, 1188, 1302,
                           1302, 1392, 1398, 1455, 1458, 1470, 1695, 1695,
                           1824, 1824, 1887, 1887, 1896, 1902, 1956, 1980,
                           2022, 2025, 2043, 2046, 2061, 2106, 2187, 2220,
                           2232, 2253, 2271, 2331, 2334, 2352, 2364, 2373,
                           2382, 2424, 2463, 2484, 2517, 2541],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,   75,
                             75,  108,  120,  249,  249,  399,  399,  507,
                            507,  567,  582,  645,  657,  690,  696,  744,
                            747,  777,  777,  786,  789,  852,  858,  972,
                            972, 1062, 1068, 1125, 1128, 1140, 1365, 1365,
                           1494, 1494, 1557, 1557, 1566, 1572, 1626, 1650,
                           1692, 1695, 1713, 1716, 1731, 1776, 1857, 1890,
                           1902, 1923, 1941, 2001, 2004, 2022, 2034, 2043,
                           2052, 2094, 2133, 2154, 2187, 2211],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,   75,
                             75,  108,  120,  249,  249,  399,  399,  507,
                            507,  567,  582,  645,  657,  690,  696,  744,
                            747,  777,  777,  786,  789,  852,  858,  972,
                            972, 1062, 1068, 1125, 1128, 1140, 1365, 1365,
                           1494, 1494, 1557, 1557, 1566, 1572, 1626, 1650,
                           1692, 1695, 1713, 1716, 1731, 1776, 1857, 1890,
                           1902, 1923, 1941, 2001, 2004, 2022, 2034, 2043,
                           2052, 2094, 2133, 2154, 2187, 2211],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,   75,
                             75,  108,  120,  249,  249,  399,  399,  507,
                            507,  567,  582,  645,  657,  690,  696,  744,
                            747,  777,  777,  786,  789,  852,  858,  972,
                            972, 1062, 1068, 1125, 1128, 1140, 1365, 1365,
                           1494, 1494, 1557, 1557, 1566, 1572, 1626, 1650,
                           1692, 1695, 1713, 1716, 1731, 1776, 1857, 1890,
                           1902, 1923, 1941, 2001, 2004, 2022, 2034, 2043,
                           2052, 2094, 2133, 2154, 2187, 2211],
                          [   0,   12,   21,  357,  378,  417,  444,  474,
                            516,  549,  564,  570,  606,  615,  642,  645,
                            795,  795,  813,  828,  918,  918,  999, 1074,
                           1077, 1110, 1110, 1239, 1251, 1401, 1428, 1536,
                           1560, 1620, 1620, 1683, 1683, 1716, 1716, 1764,
                           1764, 1794, 1809, 1818, 1818, 1881, 1881, 1995,
                           2001, 2091, 2091, 2148, 2148, 2148, 2373, 2373,
                           2502, 2532, 2595, 2619, 2628, 2628, 2682, 2706,
                           2706, 2709, 2727, 2727, 2727, 2772, 2772, 2805,
                           2817, 2817, 2835, 2895, 2898, 2916, 2928, 2937,
                           2946, 2946, 2985, 3006, 3039, 3063],
                          [   0,    0,    9,    9,   30,   30,   57,   57,
                             99,   99,  114,  114,  150,  159,  186,  189,
                            339,  339,  357,  372,  462,  465,  546,  621,
                            624,  657,  669,  798,  810,  960,  987, 1095,
                           1119, 1179, 1179, 1242, 1254, 1287, 1287, 1335,
                           1335, 1365, 1380, 1389, 1392, 1455, 1455, 1569,
                           1575, 1665, 1665, 1722, 1725, 1725, 1950, 1953,
                           2082, 2112, 2175, 2175, 2184, 2184, 2184, 2208,
                           2208, 2208, 2226, 2226, 2226, 2271, 2271, 2304,
                           2304, 2304, 2304, 2304, 2307, 2325, 2337, 2346,
                           2346, 2346, 2385, 2385, 2418, 2418],
                          [   0,    0,    9,    9,   30,   30,   57,   57,
                             99,   99,  114,  114,  150,  159,  186,  189,
                            339,  339,  357,  372,  462,  465,  546,  621,
                            624,  657,  669,  798,  810,  960,  987, 1095,
                           1119, 1179, 1179, 1242, 1254, 1287, 1287, 1335,
                           1335, 1365, 1380, 1389, 1392, 1455, 1455, 1569,
                           1575, 1665, 1665, 1722, 1725, 1725, 1950, 1953,
                           2082, 2112, 2175, 2175, 2184, 2184, 2184, 2208,
                           2208, 2208, 2226, 2229, 2229, 2229, 2229, 2262,
                           2274, 2274, 2274, 2334, 2337, 2337, 2349, 2349,
                           2349, 2349, 2388, 2388, 2388, 2388]])
                # fmt: on
            )
        )
        self.assertEqual(
            format(alignment, "clustal"),
            """\
ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         GAGGTTACTCAGAGTAGGAGGAAGCCGGTCAGAGAGGGCAGACCCTGGGA
isotig12565                         ------------GCTAGGAGA-----------------------------
isotig12566                         ------------GCTAGGAGA-----------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         ACCTTCGCAGGAGTTGGCCCAAACCCCTGAGGGAGGCTGTCCTCGGCAGG
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         AGAGCAGACCTGAGGGAGGTTGGTGTGCCGGTCCTGGTTGTCCAGCCCTC
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CCGTCCCATCCCAGGGCTGGCTCACCAGAAAAAAGTACAGGCATCCCAGG
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CCCTCTGGGGCGGGAGCAGAGGGTCTCCTCTTGTCCGGGGGAAGACAAAA
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         ACAAAAACAAAAAACGAGTGTGTAGCCCTCACTGGAAGTCTTCTGGTGCT
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CTGGGGCCGTTTGCACTTGGGAGCCTGGCTTCTGGGCATGGTGGCCGGGC
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         TCCGCCGGGGGCTTCAGCCCTCGGCAAAGCGTTTCTAGAACAGAGTGGGT
isotig12565                         -------GGCCAGGCGGCCCTCGGGAGC----------------------
isotig12566                         -------GGCCAGGCGGCCCTCGGGAGC----------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         GGGAGCCGACTGAAGTCCCAGAACCGCGAACACTGACGCACAGGAAAGCC
isotig12565                         -----------------CCAGCTGCTCGCACCTGGAGCCAGCGC------
isotig12566                         -----------------CCAGCTGCTCGCACCTGGAGCCAGCGC------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         TCGAGTGGGTGGAGAAATGCAAATCCCCCAGTGGCATGGCCTCAGCCGCT
isotig12565                         ------------------------AGCCCGGCCAGTCGGGCCTCAGCCCG
isotig12566                         ------------------------AGCCCGGCCAGTCGGGCCTCAGCCCG

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         GCGGACCCTGACGGCCGATTCTGACTTCGGACTTGAGCGGGACCAGCACC
isotig12565                         GGAGACAGTTACGCCC---------------------------------C
isotig12566                         GGAGACAGTTACGCCC---------------------------------C

ENSG00000166888:ENST0000030013      --------------------ATGTCTCTGTGGGGTCTGGTCTCCAAGATG
ENSG00000166888:ENST0000054387      --------------------ATGTCTCTGTGGGGTCTGGTCTCCAAGATG
ENSG00000166888:ENST0000055615      --------------------ATGTCTCTGTGGGGTCTGGTCTCCAAGATG
ENSG00000166888:ENST0000045407      --------------------ATGTCTCTGTGGGGTCTGGTCTCCAAGATG
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CGGACCCAGGGCGAGCCAGCATGTCTCAGTGGAATCAAGTCCAACAGTTA
isotig12565                         CTGATTGCGGCAGG------ATGGCCCAGTGGAACCAGCTGCAGCAGCTG
isotig12566                         CTGATTGCGGCAGG------ATGGCCCAGTGGAACCAGCTGCAGCAGCTG

ENSG00000166888:ENST0000030013      CCCCCA---------GAAAAAGTGCAGCGGCTCTATGTCGAC---TTTCC
ENSG00000166888:ENST0000054387      CCCCCA---------GAAAAAGTGCAGCGGCTCTATGTCGAC---TTTCC
ENSG00000166888:ENST0000055615      CCCCCA---------GAAAAAGTGCAGCGGCTCTATGTCGAC---TTTCC
ENSG00000166888:ENST0000045407      CCCCCA---------GAAAAAGTGCAGCGGCTCTATGTCGAC---TTTCC
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         GAAATCAAGTTTTTGGAGCAAGTTGATCAGTTCTATGATGACAACTTTCC
isotig12565                         GACACTCGGTACCTGGAGCAGCTGCACCAGCTGTACAGCGACAGCTTCCC
isotig12566                         GACACTCGGTACCTGGAGCAGCTGCACCAGCTGTACAGCGACAGCTTCCC

ENSG00000166888:ENST0000030013      CCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGG
ENSG00000166888:ENST0000054387      CCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGG
ENSG00000166888:ENST0000055615      CCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGG
ENSG00000166888:ENST0000045407      CCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGG
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CATGGAAATCCGACATCTGCTGGCCCAGTGGATTGAGCATCAAGACTGGG
isotig12565                         CATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGATTGGG
isotig12566                         CATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGATTGGG

ENSG00000166888:ENST0000030013      AGTTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTA
ENSG00000166888:ENST0000054387      AGTTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTA
ENSG00000166888:ENST0000055615      AGTTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTA
ENSG00000166888:ENST0000045407      AGTTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTA
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         AGGTGGCCTCTAACAATGAAACTATGGCAACAATTCTTCTTCAAAACTTA
isotig12565                         CATATGCAGCCAGCAAAGAGTCACATGCCACACTGGTGTTTCATAATCTC
isotig12566                         CATATGCAGCCAGCAAAGAGTCACATGCCACACTGGTGTTTCATAATCTC

ENSG00000166888:ENST0000030013      CTTTCAGACACTGTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGA
ENSG00000166888:ENST0000054387      CTTTCAGACACTGTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGA
ENSG00000166888:ENST0000055615      CTTTCAGACACTGTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGA
ENSG00000166888:ENST0000045407      CTTTCAGACACTGTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGA
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         TTAATACAATTGGATGAACAGTTAGGTCGTGTTTCCAAAGAGAAA-----
isotig12565                         TTGGGTGAGATTGACCAGCAGTACAGCCGATTCCTGCAAGAGTCC-----
isotig12566                         TTGGGTGAGATTGACCAGCAGTACAGCCGATTCCTGCAAGAGTCC-----

ENSG00000166888:ENST0000030013      GGGGAGCACCATCTTGCAACAC---------------ATCAGCACCCTTG
ENSG00000166888:ENST0000054387      GGGGAGCACCATCTTGCAACAC---------------ATCAGCACCCTTG
ENSG00000166888:ENST0000055615      GGGGAGCACCATCTTGCAACAC---------------ATCAGCACCCTTG
ENSG00000166888:ENST0000045407      GGGGAGCACCATCTTGCAACAC---------------ATCAGCACCCTTG
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         ----AACCTGCTATTGATCCACAATCTAAAGAGAATTAGAAAAGTACTTC
isotig12565                         ----AACGTCCTCTATCAGCACAACCTTCGGAGGATCAAGCAGTTCCTAC
isotig12566                         ----AACGTCCTCTATCAGCACAACCTTCGGAGGATCAAGCAGTTCCTAC

ENSG00000166888:ENST0000030013      AGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAA
ENSG00000166888:ENST0000054387      AGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAA
ENSG00000166888:ENST0000055615      AGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAA
ENSG00000166888:ENST0000045407      AGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAA
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         AGGGGAAGTTTCATGGAAATCCAATGCATGTAGCCGTGGTAATCTCAAAT
isotig12565                         AGAGCAGGTATCTTGAGAAGCCGATGGAAATCGCCCGCATCGTGGCCCGA
isotig12566                         AGAGCAGGTATCTTGAGAAGCCGATGGAAATCGCCCGCATCGTGGCCCGA

ENSG00000166888:ENST0000030013      ATACTTCAAGGAGAGAAAAAAGCTGTT-----------------------
ENSG00000166888:ENST0000054387      ATACTTCAAGGAGAGAAAAAAGCTGTT-----------------------
ENSG00000166888:ENST0000055615      ATACTTCAAGGAGAGAAAAAAGCTGTT-----------------------
ENSG00000166888:ENST0000045407      ATACTTCAAGGAGAGAAAAAAGCTGTT-----------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         TGTTTAAGGGAAGAGAGGAGAATACTG---GCTGCAGCGAACATGCCTAT
isotig12565                         TGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACTGCAGCCCA
isotig12566                         TGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACTGCAGCCCA

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CCAGGGACCTCTGGAGAAATCCTTACAAAGTTCGTCGGTTTCAGAAAGAC
isotig12565                         GCAAGGAGGCCAGGCCAACCACCCAACAGCTGCTGTGGTGACGGAGAAAC
isotig12566                         GCAAGGAGGCCAGGCCAACCACCCAACAGCTGCTGTGGTGACGGAGAAAC

ENSG00000166888:ENST0000030013      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000054387      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000055615      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000045407      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000053891      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000053721      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000053520      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
isotig46679                         AGAGAAATGTGGAACACAAAGTGGCTGCCATTAAAAACAGTGTGCAGATG
isotig12565                         AGCAGATGCTGGAGCAGCATCTTCAGGATGTCCGGAAACGTGTGCAGGAT
isotig12566                         AGCAGATGCTGGAGCAGCATCTTCAGGATGTCCGGAAACGTGTGCAGGAT

ENSG00000166888:ENST0000030013      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000054387      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000055615      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000045407      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000053891      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000053721      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000053520      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
isotig46679                         ACAGAACAAGACACCAAATACTTGGAAGATCTGCAAGATGAATTTGACTA
isotig12565                         CTAGAACAGAAAATGAAAGTGGTAGAGAATCTCCAGGATGACTTTGATTT
isotig12566                         CTAGAACAGAAAATGAAAGTGGTAGAGAATCTCCAGGATGACTTTGATTT

ENSG00000166888:ENST0000030013      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000054387      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000055615      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000045407      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000053891      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000053721      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000053520      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
isotig46679                         CAGGTATAAAACAATTCAGACA------------ATGGACCAGGGTGACA
isotig12565                         CAACTATAAAACCCTCAAGAGTCAAGGAGACATGCAGGATCTGAATGGAA
isotig12566                         CAACTATAAAACCCTCAAGAGTCAAGGAGACATGCAGGATCTGAATGGAA

ENSG00000166888:ENST0000030013      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000054387      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000055615      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000045407      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000053891      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000053721      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000053520      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
isotig46679                         AGAATAGCATCCTAATGAACCAGGAGGTTTTGACACTCCAAGAAATGCTT
isotig12565                         ACAACCAGTCTGTGACCAGGCAGAAGATGCAGCAGCTGGAACAGATGCTC
isotig12566                         ACAACCAGTCTGTGACCAGGCAGAAGATGCAGCAGCTGGAACAGATGCTC

ENSG00000166888:ENST0000030013      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000054387      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000055615      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000045407      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000053891      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000053721      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000053520      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
isotig46679                         AATAGCCTGGACTTCAAGAGAAAGGAAGCACTCACTAAGATGACACAGAT
isotig12565                         ACGGCGCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCT
isotig12566                         ACGGCGCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCT

ENSG00000166888:ENST0000030013      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000054387      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000055615      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000045407      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000053891      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000053721      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000053520      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
isotig46679                         AGTGAACGAGTCGGACCTGCTGATGAGCAGCATGCTCATAGAAGAGCTGC
isotig12565                         TTTGTCGGCAATGGAGTACGTGCAGAAAACACTCACAGACGAGGAGCTGG
isotig12566                         TTTGTCGGCAATGGAGTACGTGCAGAAAACACTCACAGACGAGGAGCTGG

ENSG00000166888:ENST0000030013      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000054387      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000055615      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000045407      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000053891      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000053721      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000053520      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
isotig46679                         AGGACTGGAAGAGGAGGCAGCAGATCGCCTGCATCGGTGGCCCACTCCAC
isotig12565                         CTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATTGGAGGCCCTCCCAAC
isotig12566                         CTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATTGGAGGCCCTCCCAAC

ENSG00000166888:ENST0000030013      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000054387      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000055615      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000045407      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000053891      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000053721      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000053520      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
isotig46679                         AACGGGCTGGACCAGCTTCAGAACTGCTTTACCCTGTTGGCAGAAAGTCT
isotig12565                         ATCTGCCTGGATCGCCTGGAAAACTGGATAACTTCGTTAGCAGAATCTCA
isotig12566                         ATCTGCCTGGATCGCCTGGAAAACTGGATAACTTCGTTAGCAGAATCTCA

ENSG00000166888:ENST0000030013      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000054387      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000055615      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000045407      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000053891      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000053721      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000053520      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
isotig46679                         TTTCCAACTCAGACGACAGCTGGAGAAATTAGAGGAGCAGTCTTCCAAGA
isotig12565                         ACTTCAGACCCGCCAACAAATTAAGAAACTGGAGGAGCTACAGCAGAAGG
isotig12566                         ACTTCAGACCCGCCAACAAATTAAGAAACTGGAGGAGCTACAGCAGAAGG

ENSG00000166888:ENST0000030013      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000054387      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000055615      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000045407      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000053891      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000053721      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000053520      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
isotig46679                         TGACTTACGAAGGAGACCCCATCCCCACGCAGAGAGCACACCTGCTGGAG
isotig12565                         TGTCCTACAAGGGGGACCCCATTGTGCAGCACCGGCCGATGCTGGAGGAG
isotig12566                         TGTCCTACAAGGGGGACCCCATTGTGCAGCACCGGCCGATGCTGGAGGAG

ENSG00000166888:ENST0000030013      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000054387      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000055615      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000045407      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000053891      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000053721      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000053520      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
isotig46679                         AGAGCCACCTTCCTGATCTACAACCTTTTCAAGAACTCATTTGTGGTTGA
isotig12565                         CGGATCGTGGAGCTGTTCAGAAACTTGATGAAGAGTGCCTTCGTGGTGGA
isotig12566                         CGGATCGTGGAGCTGTTCAGAAACTTGATGAAGAGTGCCTTCGTGGTGGA

ENSG00000166888:ENST0000030013      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000054387      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000055615      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000045407      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000053891      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000053721      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000053520      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
isotig46679                         GCGACAGCCCTGCATGCCAACACACCCTCAGAGGCCGCTGGTACTCAAAA
isotig12565                         GCGACAGCCCTGCATGCCGATGCACCCCGACCGGCCCTTGGTCATCAAGA
isotig12566                         GCGACAGCCCTGCATGCCGATGCACCCCGACCGGCCCTTGGTCATCAAGA

ENSG00000166888:ENST0000030013      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000054387      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000055615      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000045407      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000053891      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000053721      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000053520      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
isotig46679                         CCCTCATTCAGTTCACCGCGAAACTGAGACTACTAATAAAATTG------
isotig12565                         CTGGTGTCCAGTTCACTACTAAAGTCAGGTTGTTGGTCAAGTTT------
isotig12566                         CTGGTGTCCAGTTCACTACTAAAGTCAGGTTGTTGGTCAAGTTT------

ENSG00000166888:ENST0000030013      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000054387      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000055615      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000045407      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000053891      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000053721      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000053520      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
isotig46679                         ---------CCGGAACTCAACTATCAGGTGAAAGTAAAGGCATCGATCGA
isotig12565                         ---------CCCGAGTTGAATTATCAGCTTAAAATTAAAGTGTGCATTGA
isotig12566                         ---------CCCGAGTTGAATTATCAGCTTAAAATTAAAGTGTGCATTGA

ENSG00000166888:ENST0000030013      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000054387      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000055615      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000045407      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000053891      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000053721      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000053520      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
isotig46679                         CAAGAATGTTTCAACGCTAAGC------------AATAGAAGATTTGTGC
isotig12565                         CAAAGATTCTGGGGACGTTGCTGCTCTCAGAGGATCTCGGAAATTTAACA
isotig12566                         CAAAGATTCTGGGGACGTTGCTGCTCTCAGAGGATCTCGGAAATTTAACA

ENSG00000166888:ENST0000030013      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000054387      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000055615      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000045407      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000053891      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000053721      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000053520      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
isotig46679                         TTTGTGGAACTCAAGTC------AAAGCCATGTCCATCGAGGAATCCTCC
isotig12565                         TTCTGGGCACAAACACG------AAGGTGATGAACATGGAAGAATCCAAC
isotig12566                         TTCTGGGCACAAACACG------AAGGTGATGAACATGGAAGAATCCAAC

ENSG00000166888:ENST0000030013      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000054387      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000055615      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000045407      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000053891      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000053721      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000053520      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
isotig46679                         AATGGGAGCCTCTCAGTAGAA---TTTAGACATTTGCAACCGAAGGAAAT
isotig12565                         AACGGCAGCCTGTCTGCGGAG---TTCAAGCACTTGACCCTGAGGGAGCA
isotig12566                         AACGGCAGCCTGTCTGCGGAG---TTCAAGCACTTGACCCTGAGGGAGCA

ENSG00000166888:ENST0000030013      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000054387      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000055615      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000045407      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000053891      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000053721      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000053520      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
isotig46679                         GAAATCCAGTGCCGGAAGTAAAGGAAAT---GAGGGCTGCCACATGGTGA
isotig12565                         GAGATGTGGGAATGGAGGCCGTGCCAATTGTGATGCCTCCTTGATTGTGA
isotig12566                         GAGATGTGGGAATGGAGGCCGTGCCAATTGTGATGCCTCCTTGATTGTGA

ENSG00000166888:ENST0000030013      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000054387      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000055615      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000045407      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000053891      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000053721      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000053520      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
isotig46679                         CGGAAGAGCTGCATTCCATAGCCTTTGAGACCCAGATCTGCCTC------
isotig12565                         CCGAGGAGCTGCATCTGATCACCTTCGAGACTGAGGTGTACCAC------
isotig12566                         CCGAGGAGCTGCATCTGATCACCTTCGAGACTGAGGTGTACCAC------

ENSG00000166888:ENST0000030013      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000054387      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000055615      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000045407      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000053891      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000053721      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000053520      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
isotig46679                         TATGGCCTCACCATCGACTTGGAGACAAGCTCATTACCTGTGGTGATGAT
isotig12565                         CAAGGCCTCAAGATTGACCTGGAGACCCATTCTTTGCCAGTTGTGGTGAT
isotig12566                         CAAGGCCTCAAGATTGACCTGGAGACCCATTCTTTGCCAGTTGTGGTGAT

ENSG00000166888:ENST0000030013      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000054387      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000055615      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000045407      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000053891      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000053721      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000053520      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
isotig46679                         TTCTAATGTCAGCCAACTGCCTAATGCTTGGGCATCCATCATTTGGTACA
isotig12565                         CTCCAACATCTGTCAGATGCCAAATGCCTGGGCATCCATCCTGTGGTATA
isotig12566                         CTCCAACATCTGTCAGATGCCAAATGCCTGGGCATCCATCCTGTGGTATA

ENSG00000166888:ENST0000030013      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000054387      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000055615      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000045407      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000053891      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000053721      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000053520      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
isotig46679                         ATGTGTCAACCAACGATTGCCAGAACTTGGTTTTCTTTAATAATCCTCCG
isotig12565                         ACATGCTGACCAACAACCCCAAGAACGTGAACTTCTTCACCAAGCCACCA
isotig12566                         ACATGCTGACCAACAACCCCAAGAACGTGAACTTCTTCACCAAGCCACCA

ENSG00000166888:ENST0000030013      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000054387      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000055615      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000045407      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000053891      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000053721      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000053520      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
isotig46679                         CCTGTCACTTTGAGTCAACTCCTGGAAGTGATGAGCTGGCAGTTTTCATC
isotig12565                         ATCGGAACCTGGGACCAGGTGGCCGAGGTGCTCAGCTGGCAGTTCTCATC
isotig12566                         ATCGGAACCTGGGACCAGGTGGCCGAGGTGCTCAGCTGGCAGTTCTCATC

ENSG00000166888:ENST0000030013      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000054387      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000055615      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000045407      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000053891      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000053721      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000053520      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
isotig46679                         CTATGTTGGT------CGTGGCCTTAATTCAGACCAGCTCAACATGCTGG
isotig12565                         CACCACAAAG------CGAGGGCTGAGCATCGAGCAGCTGACTACGCTGG
isotig12566                         CACCACAAAG------CGAGGGCTGAGCATCGAGCAGCTGACTACGCTGG

ENSG00000166888:ENST0000030013      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000054387      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000055615      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000045407      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000053891      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000053721      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000053520      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
isotig46679                         CAGAGAAGCTCACAGTTCAGTCT---------------AACTACAGCGAT
isotig12565                         CCGAGAAGCTCCTAGGACCTGGTGTC------------AACTACTCCGGG
isotig12566                         CCGAGAAGCTCCTAGGACCTGGTGTC------------AACTACTCCGGG

ENSG00000166888:ENST0000030013      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000054387      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000055615      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000045407      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000053891      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000053721      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000053520      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
isotig46679                         GGTCACCTCACCTGGGCCAAGTTCTGCAAGGAACACTTGCCTGGCAAACC
isotig12565                         TGTCAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGG
isotig12566                         TGTCAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGG

ENSG00000166888:ENST0000030013      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000054387      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000055615      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000045407      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000053891      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000053721      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000053520      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
isotig46679                         ATTTACCTTCTGGACCTGGCTTGAAGCAATATTGGACCTAATTAAAAAAC
isotig12565                         CTTCTCCTTCTGGGTGTGGCTAGACAATATCATTGACCTTGTGAAAAAGT
isotig12566                         CTTCTCCTTCTGGGTGTGGCTAGACAATATCATTGACCTTGTGAAAAAGT

ENSG00000166888:ENST0000030013      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000054387      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000055615      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000045407      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000053891      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000053721      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000053520      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
isotig46679                         ACATTCTTCCCCTCTGGATTGATGGGTACATCATGGGCTTCGTGAGCAAA
isotig12565                         ATATCTTGGCCCTCTGGAATGAAGGGTACATCATGGGCTTCATTAGCAAG
isotig12566                         ATATCTTGGCCCTCTGGAATGAAGGGTACATCATGGGCTTCATTAGCAAG

ENSG00000166888:ENST0000030013      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000054387      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000055615      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000045407      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000053891      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000053721      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000053520      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
isotig46679                         GAGAAGGAGAGGTTTCTGCTCAAGGATAAAATGCCCGGGACATTTTTGTT
isotig12565                         GAGCGGGAGCGGGCGATCCTGAGCACGAAACCCCCGGGCACCTTCCTGCT
isotig12566                         GAGCGGGAGCGGGCGATCCTGAGCACGAAACCCCCGGGCACCTTCCTGCT

ENSG00000166888:ENST0000030013      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000054387      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000055615      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000045407      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000053891      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000053721      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000053520      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
isotig46679                         ACGATTCAGTGAG---AGCCATCTCGGAGGGATCACCTTCACCTGGGTGG
isotig12565                         GAGATTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACTTGGGTGG
isotig12566                         GAGATTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACTTGGGTGG

ENSG00000166888:ENST0000030013      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000054387      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000055615      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000045407      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000053891      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000053721      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000053520      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
isotig46679                         ACCACTCTGAAAACGGAGAAGTGAGATTCCACTCCGTAGAACCCTACAAC
isotig12565                         AAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCGTACACC
isotig12566                         AAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCGTACACC

ENSG00000166888:ENST0000030013      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000054387      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000055615      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000045407      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000053891      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000053721      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000053520      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
isotig46679                         AAAGGGCGTCTGTCGGCCCTGCCATTTGCTGACATCCTGCGGGACTACAA
isotig12565                         AAGCAGCAGCTGAACAACATGTCCTTTGCTGAAATCATCATGGGCTACAA
isotig12566                         AAGCAGCAGCTGAACAACATGTCCTTTGCTGAAATCATCATGGGCTACAA

ENSG00000166888:ENST0000030013      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000054387      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000055615      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000045407      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000053891      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000053721      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000053520      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
isotig46679                         GGTCATCATGGCTGAGAACATTCCCGAGAACCCTCTCAAGTACCTCTACC
isotig12565                         GATCATGGATGCCACCAACATCCTGGTGTCCCCATTGGTCTACCTCTACC
isotig12566                         GATCATGGATGCCACCAACATCCTGGTGTCCCCATTGGTCTACCTCTACC

ENSG00000166888:ENST0000030013      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000054387      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000055615      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000045407      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000053891      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000053721      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000053520      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
isotig46679                         CCGACATCCCCAAAGACAAAGCCTTCGGTAAACACTACAGCTCCCAGCCT
isotig12565                         CTGACATTCCCAAGGAGGAGGCGTTCGGGAAGTACTGT------------
isotig12566                         CTGACATTCCCAAGGAGGAGGCGTTCGGGAAGTACTGT------------

ENSG00000166888:ENST0000030013      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000054387      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000055615      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000045407      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000053891      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000053721      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000053520      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
isotig46679                         TGCGAAGTTTCAAGGCCAACA------GAACGGGGAGACAAAGGTTATGT
isotig12565                         ------------CGACCAGAG-----------------------------
isotig12566                         ------------CGACCAGAG-----------------------------

ENSG00000166888:ENST0000030013      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000054387      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000055615      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000045407      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000053891      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000053721      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000053520      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
isotig46679                         TCCTTCAGTTTTTATCCCTATTTCAACAATCCGCAGCGACGCCATGGAGC
isotig12565                         -------------------------------AGCCAGGAGCATCCTGAAG
isotig12566                         -------------------------------AGCCAGGAGCATCCTGAAG

ENSG00000166888:ENST0000030013      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000054387      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000055615      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000045407      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000053891      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000053721      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000053520      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
isotig46679                         CGCAG------------------------------------------TCT
isotig12565                         CTGAC---------------------------------------------
isotig12566                         CTGAC---------------------------------------------

ENSG00000166888:ENST0000030013      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000054387      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000055615      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000045407      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000053891      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000053721      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000053520      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
isotig46679                         CCTTCAGACCTTCTCCCC------------------ATGTCTCCGAGTGT
isotig12565                         CCCGGTAGTGCCGCCCCT------------------TACCTGAAGACCAA
isotig12566                         CCCGGTAGTTGTTTTTCCATG-----------------------------

ENSG00000166888:ENST0000030013      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000054387      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000055615      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000045407      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000053891      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000053721      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000053520      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
isotig46679                         ATACGCTGTGCTGAGAGAAAACCTGAGCCCT-------------------
isotig12565                         GTTCATCTGTGTGACACCAACGACCTGCAGC-------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000054387      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000055615      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000045407      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000053891      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000053721      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000053520      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
isotig46679                         --------------------------------------------------
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000054387      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000055615      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000045407      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000053891      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000053721      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000053520      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
isotig46679                         ------------ACCACAATTGAAACAGCAATGAAGTCTCCATATTCTGA
isotig12565                         ------------AATACCATTGACCTGCCGATGTCCCCCCCGCAC-----
isotig12566                         ------------GTTCTGGTTTCGCTGTTAGGGAAAGGGGGACAGTGCAG

ENSG00000166888:ENST0000030013      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000054387      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000055615      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000045407      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000053891      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000053721      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000053520      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
isotig46679                         GCGGTAC---------------------AAAGCGACTCTTCAAGGAAGAG
isotig12565                         --------------------------------------------------
isotig12566                         GTCCTTG---------------------------------------GAGG

ENSG00000166888:ENST0000030013      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000054387      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000055615      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000045407      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000053891      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000053721      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000053520      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
isotig46679                         AGCAGATGAAAACGGAGACTGCTCTTTGCCAAAGTCCACAATTCATTTCT
isotig12565                         --------------------------------------------------
isotig12566                         AGAGACAAGGACATGACCGGGTGTCTGGTGGTGAGTCCTGCTATGGAAGA

ENSG00000166888:ENST0000030013      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000054387      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000055615      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000045407      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000053891      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000053721      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000053520      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
isotig46679                         TCAGCTTTGATACTGGTTTCTAGAAAATGGCACAAATCCGAAGCTTTCCT
isotig12565                         ------TTTAGATTCATTGATGCAGTTTGGAAACGGAGGTGCGCCCTC--
isotig12566                         GCTGTTTAT------------------TGGGTACTTCAG-----------

ENSG00000166888:ENST0000030013      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000054387      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000055615      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000045407      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000053891      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000053721      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000053520      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
isotig46679                         CTCACTA------------------------------------------G
isotig12565                         -------------------------------------------------G
isotig12566                         -------------------------------------------------G

ENSG00000166888:ENST0000030013      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000054387      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000055615      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000045407      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000053891      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000053721      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000053520      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
isotig46679                         GTGACATTCCCCAACTGGGAGTGCTGCTGAAATGCAAACCAAAGCTTCAG
isotig12565                         GCAGGAGGGCAGTTGTCACTCACGTTCATGGATCTGAC------------
isotig12566                         GTGACCGGGATTCAAGAGAAGACCAGAATCAGGCCTCA------------

ENSG00000166888:ENST0000030013      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000054387      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000055615      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000045407      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000053891      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000053721      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000053520      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
isotig46679                         ATAAACACGCAGGAAAAGACAGCTTCGAGAAACCTATGTTCGCAATATAA
isotig12565                         ---------TTCGGAGTGCGCTACCTCCCCCATGTGAGGAGC--------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000054387      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000055615      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000045407      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000053891      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000053721      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000053520      GGCCAACCCCAGTTGG
isotig46679                         CAGAAGGCTGCTTTGC
isotig12565                         ----------------
isotig12566                         ----------------


""",
        )

    def test4(self):
        aligner = CodonAligner()
        nucleotide_records = SeqIO.index("codonalign/nucl4.fa", "fasta")
        protein_alignment = Align.read("codonalign/pro4.aln", "clustal")
        self.assertEqual(len(protein_alignment.sequences), 10)
        codon_alignments = []
        protein_record = protein_alignment.sequences[0]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 847], [0, 2541]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  S  L  W  G  L  V  S  K  M  P  P  E  K  V  Q  R  L  Y  V  
ENSG00000         0 ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC

ENSG00000        20 D  F  P  Q  H  L  R  H  L  L  G  D  W  L  E  S  Q  P  W  E  
ENSG00000        60 GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG

ENSG00000        40 F  L  V  G  S  D  A  F  C  C  N  L  A  S  A  L  L  S  D  T  
ENSG00000       120 TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT

ENSG00000        60 V  Q  H  L  Q  A  S  V  G  E  Q  G  E  G  S  T  I  L  Q  H  
ENSG00000       180 GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC

ENSG00000        80 I  S  T  L  E  S  I  Y  Q  R  D  P  L  K  L  V  A  T  F  R  
ENSG00000       240 ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA

ENSG00000       100 Q  I  L  Q  G  E  K  K  A  V  M  E  Q  F  R  H  L  P  M  P  
ENSG00000       300 CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT

ENSG00000       120 F  H  W  K  Q  E  E  L  K  F  K  T  G  L  R  R  L  Q  H  R  
ENSG00000       360 TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA

ENSG00000       140 V  G  E  I  H  L  L  R  E  A  L  Q  K  G  A  E  A  G  Q  V  
ENSG00000       420 GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG

ENSG00000       160 S  L  H  S  L  I  E  T  P  A  N  G  T  G  P  S  E  A  L  A  
ENSG00000       480 TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC

ENSG00000       180 M  L  L  Q  E  T  T  G  E  L  E  A  A  K  A  L  V  L  K  R  
ENSG00000       540 ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG

ENSG00000       200 I  Q  I  W  K  R  Q  Q  Q  L  A  G  N  G  A  P  F  E  E  S  
ENSG00000       600 ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC

ENSG00000       220 L  A  P  L  Q  E  R  C  E  S  L  V  D  I  Y  S  Q  L  Q  Q  
ENSG00000       660 CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG

ENSG00000       240 E  V  G  A  A  G  G  E  L  E  P  K  T  R  A  S  L  T  G  R  
ENSG00000       720 GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG

ENSG00000       260 L  D  E  V  L  R  T  L  V  T  S  C  F  L  V  E  K  Q  P  P  
ENSG00000       780 CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC

ENSG00000       280 Q  V  L  K  T  Q  T  K  F  Q  A  G  V  R  F  L  L  G  L  R  
ENSG00000       840 CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG

ENSG00000       300 F  L  G  A  P  A  K  P  P  L  V  R  A  D  M  V  T  E  K  Q  
ENSG00000       900 TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG

ENSG00000       320 A  R  E  L  S  V  P  Q  G  P  G  A  G  A  E  S  T  G  E  I  
ENSG00000       960 GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC

ENSG00000       340 I  N  N  T  V  P  L  E  N  S  I  P  G  N  C  C  S  A  L  F  
ENSG00000      1020 ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC

ENSG00000       360 K  N  L  L  L  K  K  I  K  R  C  E  R  K  G  T  E  S  V  T  
ENSG00000      1080 AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA

ENSG00000       380 E  E  K  C  A  V  L  F  S  A  S  F  T  L  G  P  G  K  L  P  
ENSG00000      1140 GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC

ENSG00000       400 I  Q  L  Q  A  L  S  L  P  L  V  V  I  V  H  G  N  Q  D  N  
ENSG00000      1200 ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC

ENSG00000       420 N  A  K  A  T  I  L  W  D  N  A  F  S  E  M  D  R  V  P  F  
ENSG00000      1260 AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT

ENSG00000       440 V  V  A  E  R  V  P  W  E  K  M  C  E  T  L  N  L  K  F  M  
ENSG00000      1320 GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG

ENSG00000       460 A  E  V  G  T  N  R  G  L  L  P  E  H  F  L  F  L  A  Q  K  
ENSG00000      1380 GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG

ENSG00000       480 I  F  N  D  N  S  L  S  M  E  A  F  Q  H  R  S  V  S  W  S  
ENSG00000      1440 ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG

ENSG00000       500 Q  F  N  K  E  I  L  L  G  R  G  F  T  F  W  Q  W  F  D  G  
ENSG00000      1500 CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT

ENSG00000       520 V  L  D  L  T  K  R  C  L  R  S  Y  W  S  D  R  L  I  I  G  
ENSG00000      1560 GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC

ENSG00000       540 F  I  S  K  Q  Y  V  T  S  L  L  L  N  E  P  D  G  T  F  L  
ENSG00000      1620 TTCATCAGCAAACAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTC

ENSG00000       560 L  R  F  S  D  S  E  I  G  G  I  T  I  A  H  V  I  R  G  Q  
ENSG00000      1680 CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG

ENSG00000       580 D  G  S  P  Q  I  E  N  I  Q  P  F  S  A  K  D  L  S  I  R  
ENSG00000      1740 GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC

ENSG00000       600 S  L  G  D  R  I  R  D  L  A  Q  L  K  N  L  Y  P  K  K  P  
ENSG00000      1800 TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC

ENSG00000       620 K  D  E  A  F  R  S  H  Y  K  P  E  Q  M  G  K  E  G  R  G  
ENSG00000      1860 AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT

ENSG00000       640 Y  V  P  A  T  I  K  M  T  V  E  R  D  Q  P  L  P  T  P  E  
ENSG00000      1920 TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG

ENSG00000       660 L  Q  M  P  T  M  V  P  S  Y  D  L  G  M  A  P  D  S  S  M  
ENSG00000      1980 CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG

ENSG00000       680 S  M  Q  L  G  P  D  M  V  P  Q  V  Y  P  P  H  S  H  S  I  
ENSG00000      2040 AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC

ENSG00000       700 P  P  Y  Q  G  L  S  P  E  E  S  V  N  V  L  S  A  F  Q  E  
ENSG00000      2100 CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG

ENSG00000       720 P  H  L  Q  M  P  P  S  L  G  Q  M  S  L  P  F  D  Q  P  H  
ENSG00000      2160 CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC

ENSG00000       740 P  Q  G  L  L  P  C  Q  P  Q  E  H  A  V  S  S  P  D  P  L  
ENSG00000      2220 CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG

ENSG00000       760 L  C  S  D  V  T  M  V  E  D  S  C  L  S  Q  P  V  T  A  F  
ENSG00000      2280 CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT

ENSG00000       780 P  Q  G  T  W  I  G  E  D  I  F  P  P  L  L  P  P  T  E  Q  
ENSG00000      2340 CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG

ENSG00000       800 D  L  T  K  L  L  L  E  G  Q  G  E  S  G  G  G  S  L  G  A  
ENSG00000      2400 GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA

ENSG00000       820 Q  P  L  L  Q  P  S  H  Y  G  Q  S  G  I  S  M  S  H  M  D  
ENSG00000      2460 CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC

ENSG00000       840 L  R  A  N  P  S  W    847
ENSG00000      2520 CTAAGGGCCAACCCCAGTTGG 2541
""",
        )
        protein_record = protein_alignment.sequences[1]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 847], [0, 2541]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  S  L  W  G  L  V  S  K  M  P  P  E  K  V  Q  R  L  Y  V  
ENSG00000         0 ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC

ENSG00000        20 D  F  P  Q  H  L  R  H  L  L  G  D  W  L  E  S  Q  P  W  E  
ENSG00000        60 GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG

ENSG00000        40 F  L  V  G  S  D  A  F  C  C  N  L  A  S  A  L  L  S  D  T  
ENSG00000       120 TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT

ENSG00000        60 V  Q  H  L  Q  A  S  V  G  E  Q  G  E  G  S  T  I  L  Q  H  
ENSG00000       180 GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC

ENSG00000        80 I  S  T  L  E  S  I  Y  Q  R  D  P  L  K  L  V  A  T  F  R  
ENSG00000       240 ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA

ENSG00000       100 Q  I  L  Q  G  E  K  K  A  V  M  E  Q  F  R  H  L  P  M  P  
ENSG00000       300 CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT

ENSG00000       120 F  H  W  K  Q  E  E  L  K  F  K  T  G  L  R  R  L  Q  H  R  
ENSG00000       360 TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA

ENSG00000       140 V  G  E  I  H  L  L  R  E  A  L  Q  K  G  A  E  A  G  Q  V  
ENSG00000       420 GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG

ENSG00000       160 S  L  H  S  L  I  E  T  P  A  N  G  T  G  P  S  E  A  L  A  
ENSG00000       480 TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC

ENSG00000       180 M  L  L  Q  E  T  T  G  E  L  E  A  A  K  A  L  V  L  K  R  
ENSG00000       540 ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG

ENSG00000       200 I  Q  I  W  K  R  Q  Q  Q  L  A  G  N  G  A  P  F  E  E  S  
ENSG00000       600 ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC

ENSG00000       220 L  A  P  L  Q  E  R  C  E  S  L  V  D  I  Y  S  Q  L  Q  Q  
ENSG00000       660 CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG

ENSG00000       240 E  V  G  A  A  G  G  E  L  E  P  K  T  R  A  S  L  T  G  R  
ENSG00000       720 GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG

ENSG00000       260 L  D  E  V  L  R  T  L  V  T  S  C  F  L  V  E  K  Q  P  P  
ENSG00000       780 CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC

ENSG00000       280 Q  V  L  K  T  Q  T  K  F  Q  A  G  V  R  F  L  L  G  L  R  
ENSG00000       840 CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG

ENSG00000       300 F  L  G  A  P  A  K  P  P  L  V  R  A  D  M  V  T  E  K  Q  
ENSG00000       900 TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG

ENSG00000       320 A  R  E  L  S  V  P  Q  G  P  G  A  G  A  E  S  T  G  E  I  
ENSG00000       960 GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC

ENSG00000       340 I  N  N  T  V  P  L  E  N  S  I  P  G  N  C  C  S  A  L  F  
ENSG00000      1020 ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC

ENSG00000       360 K  N  L  L  L  K  K  I  K  R  C  E  R  K  G  T  E  S  V  T  
ENSG00000      1080 AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA

ENSG00000       380 E  E  K  C  A  V  L  F  S  A  S  F  T  L  G  P  G  K  L  P  
ENSG00000      1140 GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC

ENSG00000       400 I  Q  L  Q  A  L  S  L  P  L  V  V  I  V  H  G  N  Q  D  N  
ENSG00000      1200 ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC

ENSG00000       420 N  A  K  A  T  I  L  W  D  N  A  F  S  E  M  D  R  V  P  F  
ENSG00000      1260 AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT

ENSG00000       440 V  V  A  E  R  V  P  W  E  K  M  C  E  T  L  N  L  K  F  M  
ENSG00000      1320 GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG

ENSG00000       460 A  E  V  G  T  N  R  G  L  L  P  E  H  F  L  F  L  A  Q  K  
ENSG00000      1380 GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG

ENSG00000       480 I  F  N  D  N  S  L  S  M  E  A  F  Q  H  R  S  V  S  W  S  
ENSG00000      1440 ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG

ENSG00000       500 Q  F  N  K  E  I  L  L  G  R  G  F  T  F  W  Q  W  F  D  G  
ENSG00000      1500 CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT

ENSG00000       520 V  L  D  L  T  K  R  C  L  R  S  Y  W  S  D  R  L  I  I  G  
ENSG00000      1560 GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC

ENSG00000       540 F  I  S  K  Q  Y  V  T  S  L  L  L  N  E  P  D  G  T  F  L  
ENSG00000      1620 TTCATCAGCAAACAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTC

ENSG00000       560 L  R  F  S  D  S  E  I  G  G  I  T  I  A  H  V  I  R  G  Q  
ENSG00000      1680 CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG

ENSG00000       580 D  G  S  P  Q  I  E  N  I  Q  P  F  S  A  K  D  L  S  I  R  
ENSG00000      1740 GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC

ENSG00000       600 S  L  G  D  R  I  R  D  L  A  Q  L  K  N  L  Y  P  K  K  P  
ENSG00000      1800 TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC

ENSG00000       620 K  D  E  A  F  R  S  H  Y  K  P  E  Q  M  G  K  D  G  R  G  
ENSG00000      1860 AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT

ENSG00000       640 Y  V  P  A  T  I  K  M  T  V  E  R  D  Q  P  L  P  T  P  E  
ENSG00000      1920 TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG

ENSG00000       660 L  Q  M  P  T  M  V  P  S  Y  D  L  G  M  A  P  D  S  S  M  
ENSG00000      1980 CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG

ENSG00000       680 S  M  Q  L  G  P  D  M  V  P  Q  V  Y  P  P  H  S  H  S  I  
ENSG00000      2040 AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC

ENSG00000       700 P  P  Y  Q  G  L  S  P  E  E  S  V  N  V  L  S  A  F  Q  E  
ENSG00000      2100 CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG

ENSG00000       720 P  H  L  Q  M  P  P  S  L  G  Q  M  S  L  P  F  D  Q  P  H  
ENSG00000      2160 CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC

ENSG00000       740 P  Q  G  L  L  P  C  Q  P  Q  E  H  A  V  S  S  P  D  P  L  
ENSG00000      2220 CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG

ENSG00000       760 L  C  S  D  V  T  M  V  E  D  S  C  L  S  Q  P  V  T  A  F  
ENSG00000      2280 CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT

ENSG00000       780 P  Q  G  T  W  I  G  E  D  I  F  P  P  L  L  P  P  T  E  Q  
ENSG00000      2340 CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG

ENSG00000       800 D  L  T  K  L  L  L  E  G  Q  G  E  S  G  G  G  S  L  G  A  
ENSG00000      2400 GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA

ENSG00000       820 Q  P  L  L  Q  P  S  H  Y  G  Q  S  G  I  S  M  S  H  M  D  
ENSG00000      2460 CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC

ENSG00000       840 L  R  A  N  P  S  W    847
ENSG00000      2520 CTAAGGGCCAACCCCAGTTGG 2541
""",
        )
        protein_record = protein_alignment.sequences[2]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 847], [0, 2541]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  S  L  W  G  L  V  S  K  M  P  P  E  K  V  Q  R  L  Y  V  
ENSG00000         0 ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC

ENSG00000        20 D  F  P  Q  H  L  R  H  L  L  G  D  W  L  E  S  Q  P  W  E  
ENSG00000        60 GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG

ENSG00000        40 F  L  V  G  S  D  A  F  C  C  N  L  A  S  A  L  L  S  D  T  
ENSG00000       120 TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT

ENSG00000        60 V  Q  H  L  Q  A  S  V  G  E  Q  G  E  G  S  T  I  L  Q  H  
ENSG00000       180 GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC

ENSG00000        80 I  S  T  L  E  S  I  Y  Q  R  D  P  L  K  L  V  A  T  F  R  
ENSG00000       240 ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA

ENSG00000       100 Q  I  L  Q  G  E  K  K  A  V  M  E  Q  F  R  H  L  P  M  P  
ENSG00000       300 CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT

ENSG00000       120 F  H  W  K  Q  E  E  L  K  F  K  T  G  L  R  R  L  Q  H  R  
ENSG00000       360 TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA

ENSG00000       140 V  G  E  I  H  L  L  R  E  A  L  Q  K  G  A  E  A  G  Q  V  
ENSG00000       420 GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG

ENSG00000       160 S  L  H  S  L  I  E  T  P  A  N  G  T  G  P  S  E  A  L  A  
ENSG00000       480 TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC

ENSG00000       180 M  L  L  Q  E  T  T  G  E  L  E  A  A  K  A  L  V  L  K  R  
ENSG00000       540 ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG

ENSG00000       200 I  Q  I  W  K  R  Q  Q  Q  L  A  G  N  G  A  P  F  E  E  S  
ENSG00000       600 ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC

ENSG00000       220 L  A  P  L  Q  E  R  C  E  S  L  V  D  I  Y  S  Q  L  Q  Q  
ENSG00000       660 CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG

ENSG00000       240 E  V  G  A  A  G  G  E  L  E  P  K  T  R  A  S  L  T  G  R  
ENSG00000       720 GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG

ENSG00000       260 L  D  E  V  L  R  T  L  V  T  S  C  F  L  V  E  K  Q  P  P  
ENSG00000       780 CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC

ENSG00000       280 Q  V  L  K  T  Q  T  K  F  Q  A  G  V  R  F  L  L  G  L  R  
ENSG00000       840 CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG

ENSG00000       300 F  L  G  A  P  A  K  P  P  L  V  R  A  D  M  V  T  E  K  Q  
ENSG00000       900 TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG

ENSG00000       320 A  R  E  L  S  V  P  Q  G  P  G  A  G  A  E  S  T  G  E  I  
ENSG00000       960 GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC

ENSG00000       340 I  N  N  T  V  P  L  E  N  S  I  P  G  N  C  C  S  A  L  F  
ENSG00000      1020 ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC

ENSG00000       360 K  N  L  L  L  K  K  I  K  R  C  E  R  K  G  T  E  S  V  T  
ENSG00000      1080 AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA

ENSG00000       380 E  E  K  C  A  V  L  F  S  A  S  F  T  L  G  P  G  K  L  P  
ENSG00000      1140 GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC

ENSG00000       400 I  Q  L  Q  A  L  S  L  P  L  V  V  I  V  H  G  N  Q  D  N  
ENSG00000      1200 ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC

ENSG00000       420 N  A  K  A  T  I  L  W  D  N  A  F  S  E  M  D  R  V  P  F  
ENSG00000      1260 AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT

ENSG00000       440 V  V  A  E  R  V  P  W  E  K  M  C  E  T  L  N  L  K  F  M  
ENSG00000      1320 GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG

ENSG00000       460 A  E  V  G  T  N  R  G  L  L  P  E  H  F  L  F  L  A  Q  K  
ENSG00000      1380 GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG

ENSG00000       480 I  F  N  D  N  S  L  S  M  E  A  F  Q  H  R  S  V  S  W  S  
ENSG00000      1440 ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG

ENSG00000       500 Q  F  N  K  E  I  L  L  G  R  G  F  T  F  W  Q  W  F  D  G  
ENSG00000      1500 CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT

ENSG00000       520 V  L  D  L  T  K  R  C  L  R  S  Y  W  S  D  R  L  I  I  G  
ENSG00000      1560 GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC

ENSG00000       540 F  I  S  K  Q  Y  V  T  S  L  L  L  N  E  P  D  G  T  F  L  
ENSG00000      1620 TTCATCAGCAAACAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTC

ENSG00000       560 L  R  F  S  D  S  E  I  G  G  I  T  I  A  H  V  I  R  G  Q  
ENSG00000      1680 CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG

ENSG00000       580 D  G  S  P  Q  I  E  N  I  Q  P  F  S  A  K  D  L  S  I  R  
ENSG00000      1740 GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC

ENSG00000       600 S  L  G  D  R  I  R  D  L  A  Q  L  K  N  L  Y  P  K  K  P  
ENSG00000      1800 TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC

ENSG00000       620 K  D  E  A  F  R  S  H  Y  K  P  E  Q  M  G  K  D  G  R  G  
ENSG00000      1860 AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT

ENSG00000       640 Y  V  P  A  T  I  K  M  T  V  E  R  D  Q  P  L  P  T  P  E  
ENSG00000      1920 TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG

ENSG00000       660 L  Q  M  P  T  M  V  P  S  Y  D  L  G  M  A  P  D  S  S  M  
ENSG00000      1980 CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG

ENSG00000       680 S  M  Q  L  G  P  D  M  V  P  Q  V  Y  P  P  H  S  H  S  I  
ENSG00000      2040 AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC

ENSG00000       700 P  P  Y  Q  G  L  S  P  E  E  S  V  N  V  L  S  A  F  Q  E  
ENSG00000      2100 CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG

ENSG00000       720 P  H  L  Q  M  P  P  S  L  G  Q  M  S  L  P  F  D  Q  P  H  
ENSG00000      2160 CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC

ENSG00000       740 P  Q  G  L  L  P  C  Q  P  Q  E  H  A  V  S  S  P  D  P  L  
ENSG00000      2220 CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG

ENSG00000       760 L  C  S  D  V  T  M  V  E  D  S  C  L  S  Q  P  V  T  A  F  
ENSG00000      2280 CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT

ENSG00000       780 P  Q  G  T  W  I  G  E  D  I  F  P  P  L  L  P  P  T  E  Q  
ENSG00000      2340 CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG

ENSG00000       800 D  L  T  K  L  L  L  E  G  Q  G  E  S  G  G  G  S  L  G  A  
ENSG00000      2400 GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA

ENSG00000       820 Q  P  L  L  Q  P  S  H  Y  G  Q  S  G  I  S  M  S  H  M  D  
ENSG00000      2460 CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC

ENSG00000       840 L  R  A  N  P  S  W    847
ENSG00000      2520 CTAAGGGCCAACCCCAGTTGG 2541
""",
        )
        protein_record = protein_alignment.sequences[3]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 847], [0, 2541]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  S  L  W  G  L  V  S  K  M  P  P  E  K  V  Q  R  L  Y  V  
ENSG00000         0 ATGTCTCTGTGGGGTCTGGTCTCCAAGATGCCCCCAGAAAAAGTGCAGCGGCTCTATGTC

ENSG00000        20 D  F  P  Q  H  L  R  H  L  L  G  D  W  L  E  S  Q  P  W  E  
ENSG00000        60 GACTTTCCCCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGGAG

ENSG00000        40 F  L  V  G  S  D  A  F  C  C  N  L  A  S  A  L  L  S  D  T  
ENSG00000       120 TTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTACTTTCAGACACT

ENSG00000        60 V  Q  H  L  Q  A  S  V  G  E  Q  G  E  G  S  T  I  L  Q  H  
ENSG00000       180 GTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGAGGGGAGCACCATCTTGCAACAC

ENSG00000        80 I  S  T  L  E  S  I  Y  Q  R  D  P  L  K  L  V  A  T  F  R  
ENSG00000       240 ATCAGCACCCTTGAGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGA

ENSG00000       100 Q  I  L  Q  G  E  K  K  A  V  M  E  Q  F  R  H  L  P  M  P  
ENSG00000       300 CAAATACTTCAAGGAGAGAAAAAAGCTGTTATGGAACAGTTCCGCCACTTGCCAATGCCT

ENSG00000       120 F  H  W  K  Q  E  E  L  K  F  K  T  G  L  R  R  L  Q  H  R  
ENSG00000       360 TTCCACTGGAAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGGAGGCTGCAGCACCGA

ENSG00000       140 V  G  E  I  H  L  L  R  E  A  L  Q  K  G  A  E  A  G  Q  V  
ENSG00000       420 GTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTGAGGCTGGCCAAGTG

ENSG00000       160 S  L  H  S  L  I  E  T  P  A  N  G  T  G  P  S  E  A  L  A  
ENSG00000       480 TCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGGACTGGGCCAAGTGAGGCCCTGGCC

ENSG00000       180 M  L  L  Q  E  T  T  G  E  L  E  A  A  K  A  L  V  L  K  R  
ENSG00000       540 ATGCTACTGCAGGAGACCACTGGAGAGCTAGAGGCAGCCAAAGCCCTAGTGCTGAAGAGG

ENSG00000       200 I  Q  I  W  K  R  Q  Q  Q  L  A  G  N  G  A  P  F  E  E  S  
ENSG00000       600 ATCCAGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAGGAGAGC

ENSG00000       220 L  A  P  L  Q  E  R  C  E  S  L  V  D  I  Y  S  Q  L  Q  Q  
ENSG00000       660 CTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTATTCCCAGCTACAGCAG

ENSG00000       240 E  V  G  A  A  G  G  E  L  E  P  K  T  R  A  S  L  T  G  R  
ENSG00000       720 GAGGTAGGGGCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGCCGG

ENSG00000       260 L  D  E  V  L  R  T  L  V  T  S  C  F  L  V  E  K  Q  P  P  
ENSG00000       780 CTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGAGAAGCAGCCCCCC

ENSG00000       280 Q  V  L  K  T  Q  T  K  F  Q  A  G  V  R  F  L  L  G  L  R  
ENSG00000       840 CAGGTACTGAAGACTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGG

ENSG00000       300 F  L  G  A  P  A  K  P  P  L  V  R  A  D  M  V  T  E  K  Q  
ENSG00000       900 TTCCTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGAGAAGCAG

ENSG00000       320 A  R  E  L  S  V  P  Q  G  P  G  A  G  A  E  S  T  G  E  I  
ENSG00000       960 GCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAGAAAGCACTGGAGAAATC

ENSG00000       340 I  N  N  T  V  P  L  E  N  S  I  P  G  N  C  C  S  A  L  F  
ENSG00000      1020 ATCAACAACACTGTGCCCTTGGAGAACAGCATTCCTGGGAACTGCTGCTCTGCCCTGTTC

ENSG00000       360 K  N  L  L  L  K  K  I  K  R  C  E  R  K  G  T  E  S  V  T  
ENSG00000      1080 AAGAACCTGCTTCTCAAGAAGATCAAGCGGTGTGAGCGGAAGGGCACTGAGTCTGTCACA

ENSG00000       380 E  E  K  C  A  V  L  F  S  A  S  F  T  L  G  P  G  K  L  P  
ENSG00000      1140 GAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCCGGCAAACTCCCC

ENSG00000       400 I  Q  L  Q  A  L  S  L  P  L  V  V  I  V  H  G  N  Q  D  N  
ENSG00000      1200 ATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCATCGTCCATGGCAACCAAGACAAC

ENSG00000       420 N  A  K  A  T  I  L  W  D  N  A  F  S  E  M  D  R  V  P  F  
ENSG00000      1260 AATGCCAAAGCCACTATCCTGTGGGACAATGCCTTCTCTGAGATGGACCGCGTGCCCTTT

ENSG00000       440 V  V  A  E  R  V  P  W  E  K  M  C  E  T  L  N  L  K  F  M  
ENSG00000      1320 GTGGTGGCTGAGCGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATG

ENSG00000       460 A  E  V  G  T  N  R  G  L  L  P  E  H  F  L  F  L  A  Q  K  
ENSG00000      1380 GCTGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGGCCCAGAAG

ENSG00000       480 I  F  N  D  N  S  L  S  M  E  A  F  Q  H  R  S  V  S  W  S  
ENSG00000      1440 ATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCACCGTTCTGTGTCCTGGTCG

ENSG00000       500 Q  F  N  K  E  I  L  L  G  R  G  F  T  F  W  Q  W  F  D  G  
ENSG00000      1500 CAGTTCAACAAGGAGATCCTGCTGGGCCGTGGCTTCACCTTTTGGCAGTGGTTTGATGGT

ENSG00000       520 V  L  D  L  T  K  R  C  L  R  S  Y  W  S  D  R  L  I  I  G  
ENSG00000      1560 GTCCTGGACCTCACCAAACGCTGTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGC

ENSG00000       540 F  I  S  K  Q  Y  V  T  S  L  L  L  N  E  P  D  G  T  F  L  
ENSG00000      1620 TTCATCAGCAAACAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTC

ENSG00000       560 L  R  F  S  D  S  E  I  G  G  I  T  I  A  H  V  I  R  G  Q  
ENSG00000      1680 CTCCGCTTCAGCGACTCAGAGATTGGGGGCATCACCATTGCCCATGTCATCCGGGGCCAG

ENSG00000       580 D  G  S  P  Q  I  E  N  I  Q  P  F  S  A  K  D  L  S  I  R  
ENSG00000      1740 GATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCTGCCAAAGACCTGTCCATTCGC

ENSG00000       600 S  L  G  D  R  I  R  D  L  A  Q  L  K  N  L  Y  P  K  K  P  
ENSG00000      1800 TCACTGGGGGACCGAATCCGGGATCTTGCTCAGCTCAAAAATCTCTATCCCAAGAAGCCC

ENSG00000       620 K  D  E  A  F  R  S  H  Y  K  P  E  Q  M  G  K  D  G  R  G  
ENSG00000      1860 AAGGATGAGGCTTTCCGGAGCCACTACAAGCCTGAACAGATGGGTAAGGATGGCAGGGGT

ENSG00000       640 Y  V  P  A  T  I  K  M  T  V  E  R  D  Q  P  L  P  T  P  E  
ENSG00000      1920 TATGTCCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCCCAGAG

ENSG00000       660 L  Q  M  P  T  M  V  P  S  Y  D  L  G  M  A  P  D  S  S  M  
ENSG00000      1980 CTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCCCCTGATTCCTCCATG

ENSG00000       680 S  M  Q  L  G  P  D  M  V  P  Q  V  Y  P  P  H  S  H  S  I  
ENSG00000      2040 AGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGTGTACCCACCACACTCTCACTCCATC

ENSG00000       700 P  P  Y  Q  G  L  S  P  E  E  S  V  N  V  L  S  A  F  Q  E  
ENSG00000      2100 CCCCCGTATCAAGGCCTCTCCCCAGAAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAG

ENSG00000       720 P  H  L  Q  M  P  P  S  L  G  Q  M  S  L  P  F  D  Q  P  H  
ENSG00000      2160 CCTCACCTGCAGATGCCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCAC

ENSG00000       740 P  Q  G  L  L  P  C  Q  P  Q  E  H  A  V  S  S  P  D  P  L  
ENSG00000      2220 CCCCAGGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACCCCCTG

ENSG00000       760 L  C  S  D  V  T  M  V  E  D  S  C  L  S  Q  P  V  T  A  F  
ENSG00000      2280 CTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAGCCAGTGACAGCGTTT

ENSG00000       780 P  Q  G  T  W  I  G  E  D  I  F  P  P  L  L  P  P  T  E  Q  
ENSG00000      2340 CCTCAGGGCACTTGGATTGGTGAAGACATATTCCCTCCTCTGCTGCCTCCCACTGAACAG

ENSG00000       800 D  L  T  K  L  L  L  E  G  Q  G  E  S  G  G  G  S  L  G  A  
ENSG00000      2400 GACCTCACTAAGCTTCTCCTGGAGGGGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCA

ENSG00000       820 Q  P  L  L  Q  P  S  H  Y  G  Q  S  G  I  S  M  S  H  M  D  
ENSG00000      2460 CAGCCCCTCCTGCAGCCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGAC

ENSG00000       840 L  R  A  N  P  S  W    847
ENSG00000      2520 CTAAGGGCCAACCCCAGTTGG 2541
""",
        )
        protein_record = protein_alignment.sequences[4]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 737], [0, 2211]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  E  Q  F  R  H  L  P  M  P  F  H  W  K  Q  E  E  L  K  F  
ENSG00000         0 ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGAACTCAAGTTT

ENSG00000        20 K  T  G  L  R  R  L  Q  H  R  V  G  E  I  H  L  L  R  E  A  
ENSG00000        60 AAGACAGGCTTGCGGAGGCTGCAGCACCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCC

ENSG00000        40 L  Q  K  G  A  E  A  G  Q  V  S  L  H  S  L  I  E  T  P  A  
ENSG00000       120 CTGCAGAAGGGGGCTGAGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCT

ENSG00000        60 N  G  T  G  P  S  E  A  L  A  M  L  L  Q  E  T  T  G  E  L  
ENSG00000       180 AATGGGACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGAGCTA

ENSG00000        80 E  A  A  K  A  L  V  L  K  R  I  Q  I  W  K  R  Q  Q  Q  L  
ENSG00000       240 GAGGCAGCCAAAGCCCTAGTGCTGAAGAGGATCCAGATTTGGAAACGGCAGCAGCAGCTG

ENSG00000       100 A  G  N  G  A  P  F  E  E  S  L  A  P  L  Q  E  R  C  E  S  
ENSG00000       300 GCAGGGAATGGCGCACCGTTTGAGGAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGC

ENSG00000       120 L  V  D  I  Y  S  Q  L  Q  Q  E  V  G  A  A  G  G  E  L  E  
ENSG00000       360 CTGGTGGACATTTATTCCCAGCTACAGCAGGAGGTAGGGGCGGCTGGTGGGGAGCTTGAG

ENSG00000       140 P  K  T  R  A  S  L  T  G  R  L  D  E  V  L  R  T  L  V  T  
ENSG00000       420 CCCAAGACCCGGGCATCGCTGACTGGCCGGCTGGATGAAGTCCTGAGAACCCTCGTCACC

ENSG00000       160 S  C  F  L  V  E  K  Q  P  P  Q  V  L  K  T  Q  T  K  F  Q  
ENSG00000       480 AGTTGCTTCCTGGTGGAGAAGCAGCCCCCCCAGGTACTGAAGACTCAGACCAAGTTCCAG

ENSG00000       180 A  G  V  R  F  L  L  G  L  R  F  L  G  A  P  A  K  P  P  L  
ENSG00000       540 GCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTCCTGGGGGCCCCAGCCAAGCCTCCGCTG

ENSG00000       200 V  R  A  D  M  V  T  E  K  Q  A  R  E  L  S  V  P  Q  G  P  
ENSG00000       600 GTCAGGGCCGACATGGTGACAGAGAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCT

ENSG00000       220 G  A  G  A  E  S  T  G  E  I  I  N  N  T  V  P  L  E  N  S  
ENSG00000       660 GGGGCTGGAGCAGAAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGC

ENSG00000       240 I  P  G  N  C  C  S  A  L  F  K  N  L  L  L  K  K  I  K  R  
ENSG00000       720 ATTCCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGATCAAGCGG

ENSG00000       260 C  E  R  K  G  T  E  S  V  T  E  E  K  C  A  V  L  F  S  A  
ENSG00000       780 TGTGAGCGGAAGGGCACTGAGTCTGTCACAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCC

ENSG00000       280 S  F  T  L  G  P  G  K  L  P  I  Q  L  Q  A  L  S  L  P  L  
ENSG00000       840 AGCTTCACACTTGGCCCCGGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTG

ENSG00000       300 V  V  I  V  H  G  N  Q  D  N  N  A  K  A  T  I  L  W  D  N  
ENSG00000       900 GTGGTCATCGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACAAT

ENSG00000       320 A  F  S  E  M  D  R  V  P  F  V  V  A  E  R  V  P  W  E  K  
ENSG00000       960 GCCTTCTCTGAGATGGACCGCGTGCCCTTTGTGGTGGCTGAGCGGGTGCCCTGGGAGAAG

ENSG00000       340 M  C  E  T  L  N  L  K  F  M  A  E  V  G  T  N  R  G  L  L  
ENSG00000      1020 ATGTGTGAAACTCTGAACCTGAAGTTCATGGCTGAGGTGGGGACCAACCGGGGGCTGCTC

ENSG00000       360 P  E  H  F  L  F  L  A  Q  K  I  F  N  D  N  S  L  S  M  E  
ENSG00000      1080 CCAGAGCACTTCCTCTTCCTGGCCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAG

ENSG00000       380 A  F  Q  H  R  S  V  S  W  S  Q  F  N  K  E  I  L  L  G  R  
ENSG00000      1140 GCCTTCCAGCACCGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGT

ENSG00000       400 G  F  T  F  W  Q  W  F  D  G  V  L  D  L  T  K  R  C  L  R  
ENSG00000      1200 GGCTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCTGTCTCCGG

ENSG00000       420 S  Y  W  S  D  R  L  I  I  G  F  I  S  K  Q  Y  V  T  S  L  
ENSG00000      1260 AGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAACAGTACGTTACTAGCCTT

ENSG00000       440 L  L  N  E  P  D  G  T  F  L  L  R  F  S  D  S  E  I  G  G  
ENSG00000      1320 CTTCTCAATGAGCCCGACGGAACCTTTCTCCTCCGCTTCAGCGACTCAGAGATTGGGGGC

ENSG00000       460 I  T  I  A  H  V  I  R  G  Q  D  G  S  P  Q  I  E  N  I  Q  
ENSG00000      1380 ATCACCATTGCCCATGTCATCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAG

ENSG00000       480 P  F  S  A  K  D  L  S  I  R  S  L  G  D  R  I  R  D  L  A  
ENSG00000      1440 CCATTCTCTGCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGATCTTGCT

ENSG00000       500 Q  L  K  N  L  Y  P  K  K  P  K  D  E  A  F  R  S  H  Y  K  
ENSG00000      1500 CAGCTCAAAAATCTCTATCCCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTACAAG

ENSG00000       520 P  E  Q  M  G  K  D  G  R  G  Y  V  P  A  T  I  K  M  T  V  
ENSG00000      1560 CCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCCCAGCTACCATCAAGATGACCGTG

ENSG00000       540 E  R  D  Q  P  L  P  T  P  E  L  Q  M  P  T  M  V  P  S  Y  
ENSG00000      1620 GAAAGGGACCAACCACTTCCTACCCCAGAGCTCCAGATGCCTACCATGGTGCCTTCTTAT

ENSG00000       560 D  L  G  M  A  P  D  S  S  M  S  M  Q  L  G  P  D  M  V  P  
ENSG00000      1680 GACCTTGGAATGGCCCCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCC

ENSG00000       580 Q  V  Y  P  P  H  S  H  S  I  P  P  Y  Q  G  L  S  P  E  E  
ENSG00000      1740 CAGGTGTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAGAAGAA

ENSG00000       600 S  V  N  V  L  S  A  F  Q  E  P  H  L  Q  M  P  P  S  L  G  
ENSG00000      1800 TCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATGCCCCCCAGCCTGGGC

ENSG00000       620 Q  M  S  L  P  F  D  Q  P  H  P  Q  G  L  L  P  C  Q  P  Q  
ENSG00000      1860 CAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCAGGGCCTGCTGCCGTGCCAGCCTCAG

ENSG00000       640 E  H  A  V  S  S  P  D  P  L  L  C  S  D  V  T  M  V  E  D  
ENSG00000      1920 GAGCATGCTGTGTCCAGCCCTGACCCCCTGCTCTGCTCAGATGTGACCATGGTGGAAGAC

ENSG00000       660 S  C  L  S  Q  P  V  T  A  F  P  Q  G  T  W  I  G  E  D  I  
ENSG00000      1980 AGCTGCCTGAGCCAGCCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATA

ENSG00000       680 F  P  P  L  L  P  P  T  E  Q  D  L  T  K  L  L  L  E  G  Q  
ENSG00000      2040 TTCCCTCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGGGGCAA

ENSG00000       700 G  E  S  G  G  G  S  L  G  A  Q  P  L  L  Q  P  S  H  Y  G  
ENSG00000      2100 GGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAGCCCTCCCACTATGGG

ENSG00000       720 Q  S  G  I  S  M  S  H  M  D  L  R  A  N  P  S  W    737
ENSG00000      2160 CAATCTGGGATCTCAATGTCCCACATGGACCTAAGGGCCAACCCCAGTTGG 2211
""",
        )
        protein_record = protein_alignment.sequences[5]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 737], [0, 2211]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  E  Q  F  R  H  L  P  M  P  F  H  W  K  Q  E  E  L  K  F  
ENSG00000         0 ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGAACTCAAGTTT

ENSG00000        20 K  T  G  L  R  R  L  Q  H  R  V  G  E  I  H  L  L  R  E  A  
ENSG00000        60 AAGACAGGCTTGCGGAGGCTGCAGCACCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCC

ENSG00000        40 L  Q  K  G  A  E  A  G  Q  V  S  L  H  S  L  I  E  T  P  A  
ENSG00000       120 CTGCAGAAGGGGGCTGAGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCT

ENSG00000        60 N  G  T  G  P  S  E  A  L  A  M  L  L  Q  E  T  T  G  E  L  
ENSG00000       180 AATGGGACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGAGCTA

ENSG00000        80 E  A  A  K  A  L  V  L  K  R  I  Q  I  W  K  R  Q  Q  Q  L  
ENSG00000       240 GAGGCAGCCAAAGCCCTAGTGCTGAAGAGGATCCAGATTTGGAAACGGCAGCAGCAGCTG

ENSG00000       100 A  G  N  G  A  P  F  E  E  S  L  A  P  L  Q  E  R  C  E  S  
ENSG00000       300 GCAGGGAATGGCGCACCGTTTGAGGAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGC

ENSG00000       120 L  V  D  I  Y  S  Q  L  Q  Q  E  V  G  A  A  G  G  E  L  E  
ENSG00000       360 CTGGTGGACATTTATTCCCAGCTACAGCAGGAGGTAGGGGCGGCTGGTGGGGAGCTTGAG

ENSG00000       140 P  K  T  R  A  S  L  T  G  R  L  D  E  V  L  R  T  L  V  T  
ENSG00000       420 CCCAAGACCCGGGCATCGCTGACTGGCCGGCTGGATGAAGTCCTGAGAACCCTCGTCACC

ENSG00000       160 S  C  F  L  V  E  K  Q  P  P  Q  V  L  K  T  Q  T  K  F  Q  
ENSG00000       480 AGTTGCTTCCTGGTGGAGAAGCAGCCCCCCCAGGTACTGAAGACTCAGACCAAGTTCCAG

ENSG00000       180 A  G  V  R  F  L  L  G  L  R  F  L  G  A  P  A  K  P  P  L  
ENSG00000       540 GCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTCCTGGGGGCCCCAGCCAAGCCTCCGCTG

ENSG00000       200 V  R  A  D  M  V  T  E  K  Q  A  R  E  L  S  V  P  Q  G  P  
ENSG00000       600 GTCAGGGCCGACATGGTGACAGAGAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCT

ENSG00000       220 G  A  G  A  E  S  T  G  E  I  I  N  N  T  V  P  L  E  N  S  
ENSG00000       660 GGGGCTGGAGCAGAAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGC

ENSG00000       240 I  P  G  N  C  C  S  A  L  F  K  N  L  L  L  K  K  I  K  R  
ENSG00000       720 ATTCCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGATCAAGCGG

ENSG00000       260 C  E  R  K  G  T  E  S  V  T  E  E  K  C  A  V  L  F  S  A  
ENSG00000       780 TGTGAGCGGAAGGGCACTGAGTCTGTCACAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCC

ENSG00000       280 S  F  T  L  G  P  G  K  L  P  I  Q  L  Q  A  L  S  L  P  L  
ENSG00000       840 AGCTTCACACTTGGCCCCGGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTG

ENSG00000       300 V  V  I  V  H  G  N  Q  D  N  N  A  K  A  T  I  L  W  D  N  
ENSG00000       900 GTGGTCATCGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACAAT

ENSG00000       320 A  F  S  E  M  D  R  V  P  F  V  V  A  E  R  V  P  W  E  K  
ENSG00000       960 GCCTTCTCTGAGATGGACCGCGTGCCCTTTGTGGTGGCTGAGCGGGTGCCCTGGGAGAAG

ENSG00000       340 M  C  E  T  L  N  L  K  F  M  A  E  V  G  T  N  R  G  L  L  
ENSG00000      1020 ATGTGTGAAACTCTGAACCTGAAGTTCATGGCTGAGGTGGGGACCAACCGGGGGCTGCTC

ENSG00000       360 P  E  H  F  L  F  L  A  Q  K  I  F  N  D  N  S  L  S  M  E  
ENSG00000      1080 CCAGAGCACTTCCTCTTCCTGGCCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAG

ENSG00000       380 A  F  Q  H  R  S  V  S  W  S  Q  F  N  K  E  I  L  L  G  R  
ENSG00000      1140 GCCTTCCAGCACCGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGT

ENSG00000       400 G  F  T  F  W  Q  W  F  D  G  V  L  D  L  T  K  R  C  L  R  
ENSG00000      1200 GGCTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCTGTCTCCGG

ENSG00000       420 S  Y  W  S  D  R  L  I  I  G  F  I  S  K  Q  Y  V  T  S  L  
ENSG00000      1260 AGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAACAGTACGTTACTAGCCTT

ENSG00000       440 L  L  N  E  P  D  G  T  F  L  L  R  F  S  D  S  E  I  G  G  
ENSG00000      1320 CTTCTCAATGAGCCCGACGGAACCTTTCTCCTCCGCTTCAGCGACTCAGAGATTGGGGGC

ENSG00000       460 I  T  I  A  H  V  I  R  G  Q  D  G  S  P  Q  I  E  N  I  Q  
ENSG00000      1380 ATCACCATTGCCCATGTCATCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAG

ENSG00000       480 P  F  S  A  K  D  L  S  I  R  S  L  G  D  R  I  R  D  L  A  
ENSG00000      1440 CCATTCTCTGCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGATCTTGCT

ENSG00000       500 Q  L  K  N  L  Y  P  K  K  P  K  D  E  A  F  R  S  H  Y  K  
ENSG00000      1500 CAGCTCAAAAATCTCTATCCCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTACAAG

ENSG00000       520 P  E  Q  M  G  K  D  G  R  G  Y  V  P  A  T  I  K  M  T  V  
ENSG00000      1560 CCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCCCAGCTACCATCAAGATGACCGTG

ENSG00000       540 E  R  D  Q  P  L  P  T  P  E  L  Q  M  P  T  M  V  P  S  Y  
ENSG00000      1620 GAAAGGGACCAACCACTTCCTACCCCAGAGCTCCAGATGCCTACCATGGTGCCTTCTTAT

ENSG00000       560 D  L  G  M  A  P  D  S  S  M  S  M  Q  L  G  P  D  M  V  P  
ENSG00000      1680 GACCTTGGAATGGCCCCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCC

ENSG00000       580 Q  V  Y  P  P  H  S  H  S  I  P  P  Y  Q  G  L  S  P  E  E  
ENSG00000      1740 CAGGTGTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAGAAGAA

ENSG00000       600 S  V  N  V  L  S  A  F  Q  E  P  H  L  Q  M  P  P  S  L  G  
ENSG00000      1800 TCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATGCCCCCCAGCCTGGGC

ENSG00000       620 Q  M  S  L  P  F  D  Q  P  H  P  Q  G  L  L  P  C  Q  P  Q  
ENSG00000      1860 CAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCAGGGCCTGCTGCCGTGCCAGCCTCAG

ENSG00000       640 E  H  A  V  S  S  P  D  P  L  L  C  S  D  V  T  M  V  E  D  
ENSG00000      1920 GAGCATGCTGTGTCCAGCCCTGACCCCCTGCTCTGCTCAGATGTGACCATGGTGGAAGAC

ENSG00000       660 S  C  L  S  Q  P  V  T  A  F  P  Q  G  T  W  I  G  E  D  I  
ENSG00000      1980 AGCTGCCTGAGCCAGCCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATA

ENSG00000       680 F  P  P  L  L  P  P  T  E  Q  D  L  T  K  L  L  L  E  G  Q  
ENSG00000      2040 TTCCCTCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGGGGCAA

ENSG00000       700 G  E  S  G  G  G  S  L  G  A  Q  P  L  L  Q  P  S  H  Y  G  
ENSG00000      2100 GGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAGCCCTCCCACTATGGG

ENSG00000       720 Q  S  G  I  S  M  S  H  M  D  L  R  A  N  P  S  W    737
ENSG00000      2160 CAATCTGGGATCTCAATGTCCCACATGGACCTAAGGGCCAACCCCAGTTGG 2211
""",
        )
        protein_record = protein_alignment.sequences[6]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 737], [0, 2211]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  E  Q  F  R  H  L  P  M  P  F  H  W  K  Q  E  E  L  K  F  
ENSG00000         0 ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGGAAGCAGGAAGAACTCAAGTTT

ENSG00000        20 K  T  G  L  R  R  L  Q  H  R  V  G  E  I  H  L  L  R  E  A  
ENSG00000        60 AAGACAGGCTTGCGGAGGCTGCAGCACCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCC

ENSG00000        40 L  Q  K  G  A  E  A  G  Q  V  S  L  H  S  L  I  E  T  P  A  
ENSG00000       120 CTGCAGAAGGGGGCTGAGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCT

ENSG00000        60 N  G  T  G  P  S  E  A  L  A  M  L  L  Q  E  T  T  G  E  L  
ENSG00000       180 AATGGGACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGAGCTA

ENSG00000        80 E  A  A  K  A  L  V  L  K  R  I  Q  I  W  K  R  Q  Q  Q  L  
ENSG00000       240 GAGGCAGCCAAAGCCCTAGTGCTGAAGAGGATCCAGATTTGGAAACGGCAGCAGCAGCTG

ENSG00000       100 A  G  N  G  A  P  F  E  E  S  L  A  P  L  Q  E  R  C  E  S  
ENSG00000       300 GCAGGGAATGGCGCACCGTTTGAGGAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGC

ENSG00000       120 L  V  D  I  Y  S  Q  L  Q  Q  E  V  G  A  A  G  G  E  L  E  
ENSG00000       360 CTGGTGGACATTTATTCCCAGCTACAGCAGGAGGTAGGGGCGGCTGGTGGGGAGCTTGAG

ENSG00000       140 P  K  T  R  A  S  L  T  G  R  L  D  E  V  L  R  T  L  V  T  
ENSG00000       420 CCCAAGACCCGGGCATCGCTGACTGGCCGGCTGGATGAAGTCCTGAGAACCCTCGTCACC

ENSG00000       160 S  C  F  L  V  E  K  Q  P  P  Q  V  L  K  T  Q  T  K  F  Q  
ENSG00000       480 AGTTGCTTCCTGGTGGAGAAGCAGCCCCCCCAGGTACTGAAGACTCAGACCAAGTTCCAG

ENSG00000       180 A  G  V  R  F  L  L  G  L  R  F  L  G  A  P  A  K  P  P  L  
ENSG00000       540 GCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTCCTGGGGGCCCCAGCCAAGCCTCCGCTG

ENSG00000       200 V  R  A  D  M  V  T  E  K  Q  A  R  E  L  S  V  P  Q  G  P  
ENSG00000       600 GTCAGGGCCGACATGGTGACAGAGAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCT

ENSG00000       220 G  A  G  A  E  S  T  G  E  I  I  N  N  T  V  P  L  E  N  S  
ENSG00000       660 GGGGCTGGAGCAGAAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGC

ENSG00000       240 I  P  G  N  C  C  S  A  L  F  K  N  L  L  L  K  K  I  K  R  
ENSG00000       720 ATTCCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGATCAAGCGG

ENSG00000       260 C  E  R  K  G  T  E  S  V  T  E  E  K  C  A  V  L  F  S  A  
ENSG00000       780 TGTGAGCGGAAGGGCACTGAGTCTGTCACAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCC

ENSG00000       280 S  F  T  L  G  P  G  K  L  P  I  Q  L  Q  A  L  S  L  P  L  
ENSG00000       840 AGCTTCACACTTGGCCCCGGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTG

ENSG00000       300 V  V  I  V  H  G  N  Q  D  N  N  A  K  A  T  I  L  W  D  N  
ENSG00000       900 GTGGTCATCGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACAAT

ENSG00000       320 A  F  S  E  M  D  R  V  P  F  V  V  A  E  R  V  P  W  E  K  
ENSG00000       960 GCCTTCTCTGAGATGGACCGCGTGCCCTTTGTGGTGGCTGAGCGGGTGCCCTGGGAGAAG

ENSG00000       340 M  C  E  T  L  N  L  K  F  M  A  E  V  G  T  N  R  G  L  L  
ENSG00000      1020 ATGTGTGAAACTCTGAACCTGAAGTTCATGGCTGAGGTGGGGACCAACCGGGGGCTGCTC

ENSG00000       360 P  E  H  F  L  F  L  A  Q  K  I  F  N  D  N  S  L  S  M  E  
ENSG00000      1080 CCAGAGCACTTCCTCTTCCTGGCCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAG

ENSG00000       380 A  F  Q  H  R  S  V  S  W  S  Q  F  N  K  E  I  L  L  G  R  
ENSG00000      1140 GCCTTCCAGCACCGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGT

ENSG00000       400 G  F  T  F  W  Q  W  F  D  G  V  L  D  L  T  K  R  C  L  R  
ENSG00000      1200 GGCTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCTGTCTCCGG

ENSG00000       420 S  Y  W  S  D  R  L  I  I  G  F  I  S  K  Q  Y  V  T  S  L  
ENSG00000      1260 AGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAACAGTACGTTACTAGCCTT

ENSG00000       440 L  L  N  E  P  D  G  T  F  L  L  R  F  S  D  S  E  I  G  G  
ENSG00000      1320 CTTCTCAATGAGCCCGACGGAACCTTTCTCCTCCGCTTCAGCGACTCAGAGATTGGGGGC

ENSG00000       460 I  T  I  A  H  V  I  R  G  Q  D  G  S  P  Q  I  E  N  I  Q  
ENSG00000      1380 ATCACCATTGCCCATGTCATCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAG

ENSG00000       480 P  F  S  A  K  D  L  S  I  R  S  L  G  D  R  I  R  D  L  A  
ENSG00000      1440 CCATTCTCTGCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGATCTTGCT

ENSG00000       500 Q  L  K  N  L  Y  P  K  K  P  K  D  E  A  F  R  S  H  Y  K  
ENSG00000      1500 CAGCTCAAAAATCTCTATCCCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTACAAG

ENSG00000       520 P  E  Q  M  G  K  D  G  R  G  Y  V  P  A  T  I  K  M  T  V  
ENSG00000      1560 CCTGAACAGATGGGTAAGGATGGCAGGGGTTATGTCCCAGCTACCATCAAGATGACCGTG

ENSG00000       540 E  R  D  Q  P  L  P  T  P  E  L  Q  M  P  T  M  V  P  S  Y  
ENSG00000      1620 GAAAGGGACCAACCACTTCCTACCCCAGAGCTCCAGATGCCTACCATGGTGCCTTCTTAT

ENSG00000       560 D  L  G  M  A  P  D  S  S  M  S  M  Q  L  G  P  D  M  V  P  
ENSG00000      1680 GACCTTGGAATGGCCCCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCC

ENSG00000       580 Q  V  Y  P  P  H  S  H  S  I  P  P  Y  Q  G  L  S  P  E  E  
ENSG00000      1740 CAGGTGTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAGAAGAA

ENSG00000       600 S  V  N  V  L  S  A  F  Q  E  P  H  L  Q  M  P  P  S  L  G  
ENSG00000      1800 TCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATGCCCCCCAGCCTGGGC

ENSG00000       620 Q  M  S  L  P  F  D  Q  P  H  P  Q  G  L  L  P  C  Q  P  Q  
ENSG00000      1860 CAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCAGGGCCTGCTGCCGTGCCAGCCTCAG

ENSG00000       640 E  H  A  V  S  S  P  D  P  L  L  C  S  D  V  T  M  V  E  D  
ENSG00000      1920 GAGCATGCTGTGTCCAGCCCTGACCCCCTGCTCTGCTCAGATGTGACCATGGTGGAAGAC

ENSG00000       660 S  C  L  S  Q  P  V  T  A  F  P  Q  G  T  W  I  G  E  D  I  
ENSG00000      1980 AGCTGCCTGAGCCAGCCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATA

ENSG00000       680 F  P  P  L  L  P  P  T  E  Q  D  L  T  K  L  L  L  E  G  Q  
ENSG00000      2040 TTCCCTCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGGGGCAA

ENSG00000       700 G  E  S  G  G  G  S  L  G  A  Q  P  L  L  Q  P  S  H  Y  G  
ENSG00000      2100 GGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAGCCCTCCCACTATGGG

ENSG00000       720 Q  S  G  I  S  M  S  H  M  D  L  R  A  N  P  S  W    737
ENSG00000      2160 CAATCTGGGATCTCAATGTCCCACATGGACCTAAGGGCCAACCCCAGTTGG 2211
""",
        )
        protein_record = protein_alignment.sequences[7]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        nucleotide_record = nucleotide_record.upper()
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1021], [0, 3063]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig466         0 E  V  T  Q  S  R  R  K  P  V  R  E  G  R  P  W  E  P  S  Q  
isotig466         0 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

isotig466        20 E  L  A  Q  T  P  E  G  G  C  P  R  Q  E  S  R  P  E  G  G  
isotig466        60 GAGTTGGCCCAAACCCCTGAGGGAGGCTGTCCTCGGCAGGAGAGCAGACCTGAGGGAGGT

isotig466        40 W  C  A  G  P  G  C  P  A  L  P  S  H  P  R  A  G  S  P  E  
isotig466       120 TGGTGTGCCGGTCCTGGTTGTCCAGCCCTCCCGTCCCATCCCAGGGCTGGCTCACCAGAA

isotig466        60 K  S  T  G  I  P  G  P  L  G  R  E  Q  R  V  S  S  C  P  G  
isotig466       180 AAAAGTACAGGCATCCCAGGCCCTCTGGGGCGGGAGCAGAGGGTCTCCTCTTGTCCGGGG

isotig466        80 E  D  K  N  K  N  K  K  R  V  C  S  P  H  W  K  S  S  G  A  
isotig466       240 GAAGACAAAAACAAAAACAAAAAACGAGTGTGTAGCCCTCACTGGAAGTCTTCTGGTGCT

isotig466       100 L  G  P  F  A  L  G  S  L  A  S  G  H  G  G  R  A  P  P  G  
isotig466       300 CTGGGGCCGTTTGCACTTGGGAGCCTGGCTTCTGGGCATGGTGGCCGGGCTCCGCCGGGG

isotig466       120 A  S  A  L  G  K  A  F  L  E  Q  S  G  W  E  P  T  E  V  P  
isotig466       360 GCTTCAGCCCTCGGCAAAGCGTTTCTAGAACAGAGTGGGTGGGAGCCGACTGAAGTCCCA

isotig466       140 E  P  R  T  L  T  H  R  K  A  S  S  G  W  R  N  A  N  P  P  
isotig466       420 GAACCGCGAACACTGACGCACAGGAAAGCCTCGAGTGGGTGGAGAAATGCAAATCCCCCA

isotig466       160 V  A  W  P  Q  P  L  R  T  L  T  A  D  S  D  F  G  L  E  R  
isotig466       480 GTGGCATGGCCTCAGCCGCTGCGGACCCTGACGGCCGATTCTGACTTCGGACTTGAGCGG

isotig466       180 D  Q  H  P  D  P  G  R  A  S  M  S  Q  W  N  Q  V  Q  Q  L  
isotig466       540 GACCAGCACCCGGACCCAGGGCGAGCCAGCATGTCTCAGTGGAATCAAGTCCAACAGTTA

isotig466       200 E  I  K  F  L  E  Q  V  D  Q  F  Y  D  D  N  F  P  M  E  I  
isotig466       600 GAAATCAAGTTTTTGGAGCAAGTTGATCAGTTCTATGATGACAACTTTCCCATGGAAATC

isotig466       220 R  H  L  L  A  Q  W  I  E  H  Q  D  W  E  V  A  S  N  N  E  
isotig466       660 CGACATCTGCTGGCCCAGTGGATTGAGCATCAAGACTGGGAGGTGGCCTCTAACAATGAA

isotig466       240 T  M  A  T  I  L  L  Q  N  L  L  I  Q  L  D  E  Q  L  G  R  
isotig466       720 ACTATGGCAACAATTCTTCTTCAAAACTTATTAATACAATTGGATGAACAGTTAGGTCGT

isotig466       260 V  S  K  E  K  N  L  L  L  I  H  N  L  K  R  I  R  K  V  L  
isotig466       780 GTTTCCAAAGAGAAAAACCTGCTATTGATCCACAATCTAAAGAGAATTAGAAAAGTACTT

isotig466       280 Q  G  K  F  H  G  N  P  M  H  V  A  V  V  I  S  N  C  L  R  
isotig466       840 CAGGGGAAGTTTCATGGAAATCCAATGCATGTAGCCGTGGTAATCTCAAATTGTTTAAGG

isotig466       300 E  E  R  R  I  L  A  A  A  N  M  P  I  Q  G  P  L  E  K  S  
isotig466       900 GAAGAGAGGAGAATACTGGCTGCAGCGAACATGCCTATCCAGGGACCTCTGGAGAAATCC

isotig466       320 L  Q  S  S  S  V  S  E  R  Q  R  N  V  E  H  K  V  A  A  I  
isotig466       960 TTACAAAGTTCGTCGGTTTCAGAAAGACAGAGAAATGTGGAACACAAAGTGGCTGCCATT

isotig466       340 K  N  S  V  Q  M  T  E  Q  D  T  K  Y  L  E  D  L  Q  D  E  
isotig466      1020 AAAAACAGTGTGCAGATGACAGAACAAGACACCAAATACTTGGAAGATCTGCAAGATGAA

isotig466       360 F  D  Y  R  Y  K  T  I  Q  T  M  D  Q  G  D  K  N  S  I  L  
isotig466      1080 TTTGACTACAGGTATAAAACAATTCAGACAATGGACCAGGGTGACAAGAATAGCATCCTA

isotig466       380 M  N  Q  E  V  L  T  L  Q  E  M  L  N  S  L  D  F  K  R  K  
isotig466      1140 ATGAACCAGGAGGTTTTGACACTCCAAGAAATGCTTAATAGCCTGGACTTCAAGAGAAAG

isotig466       400 E  A  L  T  K  M  T  Q  I  V  N  E  S  D  L  L  M  S  S  M  
isotig466      1200 GAAGCACTCACTAAGATGACACAGATAGTGAACGAGTCGGACCTGCTGATGAGCAGCATG

isotig466       420 L  I  E  E  L  Q  D  W  K  R  R  Q  Q  I  A  C  I  G  G  P  
isotig466      1260 CTCATAGAAGAGCTGCAGGACTGGAAGAGGAGGCAGCAGATCGCCTGCATCGGTGGCCCA

isotig466       440 L  H  N  G  L  D  Q  L  Q  N  C  F  T  L  L  A  E  S  L  F  
isotig466      1320 CTCCACAACGGGCTGGACCAGCTTCAGAACTGCTTTACCCTGTTGGCAGAAAGTCTTTTC

isotig466       460 Q  L  R  R  Q  L  E  K  L  E  E  Q  S  S  K  M  T  Y  E  G  
isotig466      1380 CAACTCAGACGACAGCTGGAGAAATTAGAGGAGCAGTCTTCCAAGATGACTTACGAAGGA

isotig466       480 D  P  I  P  T  Q  R  A  H  L  L  E  R  A  T  F  L  I  Y  N  
isotig466      1440 GACCCCATCCCCACGCAGAGAGCACACCTGCTGGAGAGAGCCACCTTCCTGATCTACAAC

isotig466       500 L  F  K  N  S  F  V  V  E  R  Q  P  C  M  P  T  H  P  Q  R  
isotig466      1500 CTTTTCAAGAACTCATTTGTGGTTGAGCGACAGCCCTGCATGCCAACACACCCTCAGAGG

isotig466       520 P  L  V  L  K  T  L  I  Q  F  T  A  K  L  R  L  L  I  K  L  
isotig466      1560 CCGCTGGTACTCAAAACCCTCATTCAGTTCACCGCGAAACTGAGACTACTAATAAAATTG

isotig466       540 P  E  L  N  Y  Q  V  K  V  K  A  S  I  D  K  N  V  S  T  L  
isotig466      1620 CCGGAACTCAACTATCAGGTGAAAGTAAAGGCATCGATCGACAAGAATGTTTCAACGCTA

isotig466       560 S  N  R  R  F  V  L  C  G  T  Q  V  K  A  M  S  I  E  E  S  
isotig466      1680 AGCAATAGAAGATTTGTGCTTTGTGGAACTCAAGTCAAAGCCATGTCCATCGAGGAATCC

isotig466       580 S  N  G  S  L  S  V  E  F  R  H  L  Q  P  K  E  M  K  S  S  
isotig466      1740 TCCAATGGGAGCCTCTCAGTAGAATTTAGACATTTGCAACCGAAGGAAATGAAATCCAGT

isotig466       600 A  G  S  K  G  N  E  G  C  H  M  V  T  E  E  L  H  S  I  A  
isotig466      1800 GCCGGAAGTAAAGGAAATGAGGGCTGCCACATGGTGACGGAAGAGCTGCATTCCATAGCC

isotig466       620 F  E  T  Q  I  C  L  Y  G  L  T  I  D  L  E  T  S  S  L  P  
isotig466      1860 TTTGAGACCCAGATCTGCCTCTATGGCCTCACCATCGACTTGGAGACAAGCTCATTACCT

isotig466       640 V  V  M  I  S  N  V  S  Q  L  P  N  A  W  A  S  I  I  W  Y  
isotig466      1920 GTGGTGATGATTTCTAATGTCAGCCAACTGCCTAATGCTTGGGCATCCATCATTTGGTAC

isotig466       660 N  V  S  T  N  D  C  Q  N  L  V  F  F  N  N  P  P  P  V  T  
isotig466      1980 AATGTGTCAACCAACGATTGCCAGAACTTGGTTTTCTTTAATAATCCTCCGCCTGTCACT

isotig466       680 L  S  Q  L  L  E  V  M  S  W  Q  F  S  S  Y  V  G  R  G  L  
isotig466      2040 TTGAGTCAACTCCTGGAAGTGATGAGCTGGCAGTTTTCATCCTATGTTGGTCGTGGCCTT

isotig466       700 N  S  D  Q  L  N  M  L  A  E  K  L  T  V  Q  S  N  Y  S  D  
isotig466      2100 AATTCAGACCAGCTCAACATGCTGGCAGAGAAGCTCACAGTTCAGTCTAACTACAGCGAT

isotig466       720 G  H  L  T  W  A  K  F  C  K  E  H  L  P  G  K  P  F  T  F  
isotig466      2160 GGTCACCTCACCTGGGCCAAGTTCTGCAAGGAACACTTGCCTGGCAAACCATTTACCTTC

isotig466       740 W  T  W  L  E  A  I  L  D  L  I  K  K  H  I  L  P  L  W  I  
isotig466      2220 TGGACCTGGCTTGAAGCAATATTGGACCTAATTAAAAAACACATTCTTCCCCTCTGGATT

isotig466       760 D  G  Y  I  M  G  F  V  S  K  E  K  E  R  F  L  L  K  D  K  
isotig466      2280 GATGGGTACATCATGGGCTTCGTGAGCAAAGAGAAGGAGAGGTTTCTGCTCAAGGATAAA

isotig466       780 M  P  G  T  F  L  L  R  F  S  E  S  H  L  G  G  I  T  F  T  
isotig466      2340 ATGCCCGGGACATTTTTGTTACGATTCAGTGAGAGCCATCTCGGAGGGATCACCTTCACC

isotig466       800 W  V  D  H  S  E  N  G  E  V  R  F  H  S  V  E  P  Y  N  K  
isotig466      2400 TGGGTGGACCACTCTGAAAACGGAGAAGTGAGATTCCACTCCGTAGAACCCTACAACAAA

isotig466       820 G  R  L  S  A  L  P  F  A  D  I  L  R  D  Y  K  V  I  M  A  
isotig466      2460 GGGCGTCTGTCGGCCCTGCCATTTGCTGACATCCTGCGGGACTACAAGGTCATCATGGCT

isotig466       840 E  N  I  P  E  N  P  L  K  Y  L  Y  P  D  I  P  K  D  K  A  
isotig466      2520 GAGAACATTCCCGAGAACCCTCTCAAGTACCTCTACCCCGACATCCCCAAAGACAAAGCC

isotig466       860 F  G  K  H  Y  S  S  Q  P  C  E  V  S  R  P  T  E  R  G  D  
isotig466      2580 TTCGGTAAACACTACAGCTCCCAGCCTTGCGAAGTTTCAAGGCCAACAGAACGGGGAGAC

isotig466       880 K  G  Y  V  P  S  V  F  I  P  I  S  T  I  R  S  D  A  M  E  
isotig466      2640 AAAGGTTATGTTCCTTCAGTTTTTATCCCTATTTCAACAATCCGCAGCGACGCCATGGAG

isotig466       900 P  Q  S  P  S  D  L  L  P  M  S  P  S  V  Y  A  V  L  R  E  
isotig466      2700 CCGCAGTCTCCTTCAGACCTTCTCCCCATGTCTCCGAGTGTATACGCTGTGCTGAGAGAA

isotig466       920 N  L  S  P  T  T  I  E  T  A  M  K  S  P  Y  S  E  R  Y  K  
isotig466      2760 AACCTGAGCCCTACCACAATTGAAACAGCAATGAAGTCTCCATATTCTGAGCGGTACAAA

isotig466       940 A  T  L  Q  G  R  E  Q  M  K  T  E  T  A  L  C  Q  S  P  Q  
isotig466      2820 GCGACTCTTCAAGGAAGAGAGCAGATGAAAACGGAGACTGCTCTTTGCCAAAGTCCACAA

isotig466       960 F  I  S  S  A  L  I  L  V  S  R  K  W  H  K  S  E  A  F  L  
isotig466      2880 TTCATTTCTTCAGCTTTGATACTGGTTTCTAGAAAATGGCACAAATCCGAAGCTTTCCTC

isotig466       980 S  L  G  D  I  P  Q  L  G  V  L  L  K  C  K  P  K  L  Q  I  
isotig466      2940 TCACTAGGTGACATTCCCCAACTGGGAGTGCTGCTGAAATGCAAACCAAAGCTTCAGATA

isotig466      1000 N  T  Q  E  K  T  A  S  R  N  L  C  S  Q  Y  N  R  R  L  L  
isotig466      3000 AACACGCAGGAAAAGACAGCTTCGAGAAACCTATGTTCGCAATATAACAGAAGGCTGCTT

isotig466      1020 C   1021
isotig466      3060 TGC 3063
""",
        )
        protein_record = protein_alignment.sequences[8]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        nucleotide_record = nucleotide_record.upper()
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 806], [0, 2418]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig125         0 A  R  R  G  Q  A  A  L  G  S  P  A  A  R  T  W  S  Q  R  S  
isotig125         0 GCTAGGAGAGGCCAGGCGGCCCTCGGGAGCCCAGCTGCTCGCACCTGGAGCCAGCGCAGC

isotig125        20 P  A  S  R  A  S  A  R  E  T  V  T  P  P  D  C  G  R  M  A  
isotig125        60 CCGGCCAGTCGGGCCTCAGCCCGGGAGACAGTTACGCCCCCTGATTGCGGCAGGATGGCC

isotig125        40 Q  W  N  Q  L  Q  Q  L  D  T  R  Y  L  E  Q  L  H  Q  L  Y  
isotig125       120 CAGTGGAACCAGCTGCAGCAGCTGGACACTCGGTACCTGGAGCAGCTGCACCAGCTGTAC

isotig125        60 S  D  S  F  P  M  E  L  R  Q  F  L  A  P  W  I  E  S  Q  D  
isotig125       180 AGCGACAGCTTCCCCATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGAT

isotig125        80 W  A  Y  A  A  S  K  E  S  H  A  T  L  V  F  H  N  L  L  G  
isotig125       240 TGGGCATATGCAGCCAGCAAAGAGTCACATGCCACACTGGTGTTTCATAATCTCTTGGGT

isotig125       100 E  I  D  Q  Q  Y  S  R  F  L  Q  E  S  N  V  L  Y  Q  H  N  
isotig125       300 GAGATTGACCAGCAGTACAGCCGATTCCTGCAAGAGTCCAACGTCCTCTATCAGCACAAC

isotig125       120 L  R  R  I  G  Q  F  V  Q  S  R  Y  L  E  K  P  M  E  I  A  
isotig125       360 CTTCGGAGGATCAAGCAGTTCCTACAGAGCAGGTATCTTGAGAAGCCGATGGAAATCGCC

isotig125       140 R  I  V  A  R  C  L  W  E  E  S  R  L  L  Q  T  A  A  T  A  
isotig125       420 CGCATCGTGGCCCGATGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACTGCA

isotig125       160 A  Q  Q  G  G  Q  A  N  H  P  T  A  A  V  V  T  E  K  Q  Q  
isotig125       480 GCCCAGCAAGGAGGCCAGGCCAACCACCCAACAGCTGCTGTGGTGACGGAGAAACAGCAG

isotig125       180 M  L  E  Q  H  L  Q  D  V  R  K  R  V  Q  D  L  E  Q  K  M  
isotig125       540 ATGCTGGAGCAGCATCTTCAGGATGTCCGGAAACGTGTGCAGGATCTAGAACAGAAAATG

isotig125       200 K  V  V  E  N  L  Q  D  D  F  D  F  N  Y  K  T  L  K  S  Q  
isotig125       600 AAAGTGGTAGAGAATCTCCAGGATGACTTTGATTTCAACTATAAAACCCTCAAGAGTCAA

isotig125       220 G  D  M  Q  D  L  N  G  N  N  Q  S  V  T  R  Q  K  M  Q  Q  
isotig125       660 GGAGACATGCAGGATCTGAATGGAAACAACCAGTCTGTGACCAGGCAGAAGATGCAGCAG

isotig125       240 L  E  Q  M  L  T  A  L  D  Q  M  R  R  S  I  V  S  E  L  A  
isotig125       720 CTGGAACAGATGCTCACGGCGCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCG

isotig125       260 G  L  L  S  A  M  E  Y  V  Q  K  T  L  T  D  E  E  L  A  D  
isotig125       780 GGGCTTTTGTCGGCAATGGAGTACGTGCAGAAAACACTCACAGACGAGGAGCTGGCTGAC

isotig125       280 W  K  R  R  Q  Q  I  A  C  I  G  G  P  P  N  I  C  L  D  R  
isotig125       840 TGGAAGAGGCGGCAGCAGATCGCGTGCATTGGAGGCCCTCCCAACATCTGCCTGGATCGC

isotig125       300 L  E  N  W  I  T  S  L  A  E  S  Q  L  Q  T  R  Q  Q  I  K  
isotig125       900 CTGGAAAACTGGATAACTTCGTTAGCAGAATCTCAACTTCAGACCCGCCAACAAATTAAG

isotig125       320 K  L  E  E  L  Q  Q  K  V  S  Y  K  G  D  P  I  V  Q  H  R  
isotig125       960 AAACTGGAGGAGCTACAGCAGAAGGTGTCCTACAAGGGGGACCCCATTGTGCAGCACCGG

isotig125       340 P  M  L  E  E  R  I  V  E  L  F  R  N  L  M  K  S  A  F  V  
isotig125      1020 CCGATGCTGGAGGAGCGGATCGTGGAGCTGTTCAGAAACTTGATGAAGAGTGCCTTCGTG

isotig125       360 V  E  R  Q  P  C  M  P  M  H  P  D  R  P  L  V  I  K  T  G  
isotig125      1080 GTGGAGCGACAGCCCTGCATGCCGATGCACCCCGACCGGCCCTTGGTCATCAAGACTGGT

isotig125       380 V  Q  F  T  T  K  V  R  L  L  V  K  F  P  E  L  N  Y  Q  L  
isotig125      1140 GTCCAGTTCACTACTAAAGTCAGGTTGTTGGTCAAGTTTCCCGAGTTGAATTATCAGCTT

isotig125       400 K  I  K  V  C  I  D  K  D  S  G  D  V  A  A  L  R  G  S  R  
isotig125      1200 AAAATTAAAGTGTGCATTGACAAAGATTCTGGGGACGTTGCTGCTCTCAGAGGATCTCGG

isotig125       420 K  F  N  I  L  G  T  N  T  K  V  M  N  M  E  E  S  N  N  G  
isotig125      1260 AAATTTAACATTCTGGGCACAAACACGAAGGTGATGAACATGGAAGAATCCAACAACGGC

isotig125       440 S  L  S  A  E  F  K  H  L  T  L  R  E  Q  R  C  G  N  G  G  
isotig125      1320 AGCCTGTCTGCGGAGTTCAAGCACTTGACCCTGAGGGAGCAGAGATGTGGGAATGGAGGC

isotig125       460 R  A  N  C  D  A  S  L  I  V  T  E  E  L  H  L  I  T  F  E  
isotig125      1380 CGTGCCAATTGTGATGCCTCCTTGATTGTGACCGAGGAGCTGCATCTGATCACCTTCGAG

isotig125       480 T  E  V  Y  H  Q  G  L  K  I  D  L  E  T  H  S  L  P  V  V  
isotig125      1440 ACTGAGGTGTACCACCAAGGCCTCAAGATTGACCTGGAGACCCATTCTTTGCCAGTTGTG

isotig125       500 V  I  S  N  I  C  Q  M  P  N  A  W  A  S  I  L  W  Y  N  M  
isotig125      1500 GTGATCTCCAACATCTGTCAGATGCCAAATGCCTGGGCATCCATCCTGTGGTATAACATG

isotig125       520 L  T  N  N  P  K  N  V  N  F  F  T  K  P  P  I  G  T  W  D  
isotig125      1560 CTGACCAACAACCCCAAGAACGTGAACTTCTTCACCAAGCCACCAATCGGAACCTGGGAC

isotig125       540 Q  V  A  E  V  L  S  W  Q  F  S  S  T  T  K  R  G  L  S  I  
isotig125      1620 CAGGTGGCCGAGGTGCTCAGCTGGCAGTTCTCATCCACCACAAAGCGAGGGCTGAGCATC

isotig125       560 E  Q  L  T  T  L  A  E  K  L  L  G  P  G  V  N  Y  S  G  C  
isotig125      1680 GAGCAGCTGACTACGCTGGCCGAGAAGCTCCTAGGACCTGGTGTCAACTACTCCGGGTGT

isotig125       580 Q  I  T  W  A  K  F  C  K  E  N  M  A  G  K  G  F  S  F  W  
isotig125      1740 CAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCCTTCTGG

isotig125       600 V  W  L  D  N  I  I  D  L  V  K  K  Y  I  L  A  L  W  N  E  
isotig125      1800 GTGTGGCTAGACAATATCATTGACCTTGTGAAAAAGTATATCTTGGCCCTCTGGAATGAA

isotig125       620 G  Y  I  M  G  F  I  S  K  E  R  E  R  A  I  L  S  T  K  P  
isotig125      1860 GGGTACATCATGGGCTTCATTAGCAAGGAGCGGGAGCGGGCGATCCTGAGCACGAAACCC

isotig125       640 P  G  T  F  L  L  R  F  S  E  S  S  K  E  G  G  V  T  F  T  
isotig125      1920 CCGGGCACCTTCCTGCTGAGATTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACT

isotig125       660 W  V  E  K  D  I  S  G  K  T  Q  I  Q  S  V  E  P  Y  T  K  
isotig125      1980 TGGGTGGAAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCGTACACCAAG

isotig125       680 Q  Q  L  N  N  M  S  F  A  E  I  I  M  G  Y  K  I  M  D  A  
isotig125      2040 CAGCAGCTGAACAACATGTCCTTTGCTGAAATCATCATGGGCTACAAGATCATGGATGCC

isotig125       700 T  N  I  L  V  S  P  L  V  Y  L  Y  P  D  I  P  K  E  E  A  
isotig125      2100 ACCAACATCCTGGTGTCCCCATTGGTCTACCTCTACCCTGACATTCCCAAGGAGGAGGCG

isotig125       720 F  G  K  Y  C  R  P  E  S  Q  E  H  P  E  A  D  P  G  S  A  
isotig125      2160 TTCGGGAAGTACTGTCGACCAGAGAGCCAGGAGCATCCTGAAGCTGACCCCGGTAGTGCC

isotig125       740 A  P  Y  L  K  T  K  F  I  C  V  T  P  T  T  C  S  N  T  I  
isotig125      2220 GCCCCTTACCTGAAGACCAAGTTCATCTGTGTGACACCAACGACCTGCAGCAATACCATT

isotig125       760 D  L  P  M  S  P  P  H  F  R  F  I  D  A  V  W  K  R  R  C  
isotig125      2280 GACCTGCCGATGTCCCCCCCGCACTTTAGATTCATTGATGCAGTTTGGAAACGGAGGTGC

isotig125       780 A  L  G  R  R  A  V  V  T  H  V  H  G  S  D  F  G  V  R  Y  
isotig125      2340 GCCCTCGGCAGGAGGGCAGTTGTCACTCACGTTCATGGATCTGACTTCGGAGTGCGCTAC

isotig125       800 L  P  H  V  R  S    806
isotig125      2400 CTCCCCCATGTGAGGAGC 2418
""",
        )
        protein_record = protein_alignment.sequences[9]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        nucleotide_record = nucleotide_record.upper()
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 796], [0, 2388]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig125         0 A  R  R  G  Q  A  A  L  G  S  P  A  A  R  T  W  S  Q  R  S  
isotig125         0 GCTAGGAGAGGCCAGGCGGCCCTCGGGAGCCCAGCTGCTCGCACCTGGAGCCAGCGCAGC

isotig125        20 P  A  S  R  A  S  A  R  E  T  V  T  P  P  D  C  G  R  M  A  
isotig125        60 CCGGCCAGTCGGGCCTCAGCCCGGGAGACAGTTACGCCCCCTGATTGCGGCAGGATGGCC

isotig125        40 Q  W  N  Q  L  Q  Q  L  D  T  R  Y  L  E  Q  L  H  Q  L  Y  
isotig125       120 CAGTGGAACCAGCTGCAGCAGCTGGACACTCGGTACCTGGAGCAGCTGCACCAGCTGTAC

isotig125        60 S  D  S  F  P  M  E  L  R  Q  F  L  A  P  W  I  E  S  Q  D  
isotig125       180 AGCGACAGCTTCCCCATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGAT

isotig125        80 W  A  Y  A  A  S  K  E  S  H  A  T  L  V  F  H  N  L  L  G  
isotig125       240 TGGGCATATGCAGCCAGCAAAGAGTCACATGCCACACTGGTGTTTCATAATCTCTTGGGT

isotig125       100 E  I  D  Q  Q  Y  S  R  F  L  Q  E  S  N  V  L  Y  Q  H  N  
isotig125       300 GAGATTGACCAGCAGTACAGCCGATTCCTGCAAGAGTCCAACGTCCTCTATCAGCACAAC

isotig125       120 L  R  R  I  K  Q  F  L  Q  S  R  Y  L  E  K  P  M  E  I  A  
isotig125       360 CTTCGGAGGATCAAGCAGTTCCTACAGAGCAGGTATCTTGAGAAGCCGATGGAAATCGCC

isotig125       140 R  I  V  A  R  C  L  W  E  E  S  R  L  L  Q  T  A  A  T  A  
isotig125       420 CGCATCGTGGCCCGATGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACTGCA

isotig125       160 A  Q  Q  G  G  Q  A  N  H  P  T  A  A  V  V  T  E  K  Q  Q  
isotig125       480 GCCCAGCAAGGAGGCCAGGCCAACCACCCAACAGCTGCTGTGGTGACGGAGAAACAGCAG

isotig125       180 M  L  E  Q  H  L  Q  D  V  R  K  R  V  Q  D  L  E  Q  K  M  
isotig125       540 ATGCTGGAGCAGCATCTTCAGGATGTCCGGAAACGTGTGCAGGATCTAGAACAGAAAATG

isotig125       200 K  V  V  E  N  L  Q  D  D  F  D  F  N  Y  K  T  L  K  S  Q  
isotig125       600 AAAGTGGTAGAGAATCTCCAGGATGACTTTGATTTCAACTATAAAACCCTCAAGAGTCAA

isotig125       220 G  D  M  Q  D  L  N  G  N  N  Q  S  V  T  R  Q  K  M  Q  Q  
isotig125       660 GGAGACATGCAGGATCTGAATGGAAACAACCAGTCTGTGACCAGGCAGAAGATGCAGCAG

isotig125       240 L  E  Q  M  L  T  A  L  D  Q  M  R  R  S  I  V  S  E  L  A  
isotig125       720 CTGGAACAGATGCTCACGGCGCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCG

isotig125       260 G  L  L  S  A  M  E  Y  V  Q  K  T  L  T  D  E  E  L  A  D  
isotig125       780 GGGCTTTTGTCGGCAATGGAGTACGTGCAGAAAACACTCACAGACGAGGAGCTGGCTGAC

isotig125       280 W  K  R  R  Q  Q  I  A  C  I  G  G  P  P  N  I  C  L  D  R  
isotig125       840 TGGAAGAGGCGGCAGCAGATCGCGTGCATTGGAGGCCCTCCCAACATCTGCCTGGATCGC

isotig125       300 L  E  N  W  I  T  S  L  A  E  S  Q  L  Q  T  R  Q  Q  I  K  
isotig125       900 CTGGAAAACTGGATAACTTCGTTAGCAGAATCTCAACTTCAGACCCGCCAACAAATTAAG

isotig125       320 K  L  E  E  L  Q  Q  K  V  S  Y  K  G  D  P  I  V  Q  H  R  
isotig125       960 AAACTGGAGGAGCTACAGCAGAAGGTGTCCTACAAGGGGGACCCCATTGTGCAGCACCGG

isotig125       340 P  M  L  E  E  R  I  V  E  L  F  R  N  L  M  K  S  A  F  V  
isotig125      1020 CCGATGCTGGAGGAGCGGATCGTGGAGCTGTTCAGAAACTTGATGAAGAGTGCCTTCGTG

isotig125       360 V  E  R  Q  P  C  M  P  M  H  P  D  R  P  L  V  I  K  T  G  
isotig125      1080 GTGGAGCGACAGCCCTGCATGCCGATGCACCCCGACCGGCCCTTGGTCATCAAGACTGGT

isotig125       380 V  Q  F  T  T  K  V  R  L  L  V  K  F  P  E  L  N  Y  Q  L  
isotig125      1140 GTCCAGTTCACTACTAAAGTCAGGTTGTTGGTCAAGTTTCCCGAGTTGAATTATCAGCTT

isotig125       400 K  I  K  V  C  I  D  K  D  S  G  D  V  A  A  L  R  G  S  R  
isotig125      1200 AAAATTAAAGTGTGCATTGACAAAGATTCTGGGGACGTTGCTGCTCTCAGAGGATCTCGG

isotig125       420 K  F  N  I  L  G  T  N  T  K  V  M  N  M  E  E  S  N  N  G  
isotig125      1260 AAATTTAACATTCTGGGCACAAACACGAAGGTGATGAACATGGAAGAATCCAACAACGGC

isotig125       440 S  L  S  A  E  F  K  H  L  T  L  R  E  Q  R  C  G  N  G  G  
isotig125      1320 AGCCTGTCTGCGGAGTTCAAGCACTTGACCCTGAGGGAGCAGAGATGTGGGAATGGAGGC

isotig125       460 R  A  N  C  D  A  S  L  I  V  T  E  E  L  H  L  I  T  F  E  
isotig125      1380 CGTGCCAATTGTGATGCCTCCTTGATTGTGACCGAGGAGCTGCATCTGATCACCTTCGAG

isotig125       480 T  E  V  Y  H  Q  G  L  K  I  D  L  E  T  H  S  L  P  V  V  
isotig125      1440 ACTGAGGTGTACCACCAAGGCCTCAAGATTGACCTGGAGACCCATTCTTTGCCAGTTGTG

isotig125       500 V  I  S  N  I  C  Q  M  P  N  A  W  A  S  I  L  W  Y  N  M  
isotig125      1500 GTGATCTCCAACATCTGTCAGATGCCAAATGCCTGGGCATCCATCCTGTGGTATAACATG

isotig125       520 L  T  N  N  P  K  N  V  N  F  F  T  K  P  P  I  G  T  W  D  
isotig125      1560 CTGACCAACAACCCCAAGAACGTGAACTTCTTCACCAAGCCACCAATCGGAACCTGGGAC

isotig125       540 Q  V  A  E  V  L  S  W  Q  F  S  S  T  T  K  R  G  L  S  I  
isotig125      1620 CAGGTGGCCGAGGTGCTCAGCTGGCAGTTCTCATCCACCACAAAGCGAGGGCTGAGCATC

isotig125       560 E  Q  L  T  T  L  A  E  K  L  L  G  P  G  V  N  Y  S  G  C  
isotig125      1680 GAGCAGCTGACTACGCTGGCCGAGAAGCTCCTAGGACCTGGTGTCAACTACTCCGGGTGT

isotig125       580 Q  I  T  W  A  K  F  C  K  E  N  M  A  G  K  G  F  S  F  W  
isotig125      1740 CAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCCTTCTGG

isotig125       600 V  W  L  D  N  I  I  D  L  V  K  K  Y  I  L  A  L  W  N  E  
isotig125      1800 GTGTGGCTAGACAATATCATTGACCTTGTGAAAAAGTATATCTTGGCCCTCTGGAATGAA

isotig125       620 G  Y  I  M  G  F  I  S  K  E  R  E  R  A  I  L  S  T  K  P  
isotig125      1860 GGGTACATCATGGGCTTCATTAGCAAGGAGCGGGAGCGGGCGATCCTGAGCACGAAACCC

isotig125       640 P  G  T  F  L  L  R  F  S  E  S  S  K  E  G  G  V  T  F  T  
isotig125      1920 CCGGGCACCTTCCTGCTGAGATTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACT

isotig125       660 W  V  E  K  D  I  S  G  K  T  Q  I  Q  S  V  E  P  Y  T  K  
isotig125      1980 TGGGTGGAAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCGTACACCAAG

isotig125       680 Q  Q  L  N  N  M  S  F  A  E  I  I  M  G  Y  K  I  M  D  A  
isotig125      2040 CAGCAGCTGAACAACATGTCCTTTGCTGAAATCATCATGGGCTACAAGATCATGGATGCC

isotig125       700 T  N  I  L  V  S  P  L  V  Y  L  Y  P  D  I  P  K  E  E  A  
isotig125      2100 ACCAACATCCTGGTGTCCCCATTGGTCTACCTCTACCCTGACATTCCCAAGGAGGAGGCG

isotig125       720 F  G  K  Y  C  R  P  E  S  Q  E  H  P  E  A  D  P  G  S  C  
isotig125      2160 TTCGGGAAGTACTGTCGACCAGAGAGCCAGGAGCATCCTGAAGCTGACCCCGGTAGTTGT

isotig125       740 F  S  M  V  L  V  S  L  L  G  K  G  G  Q  C  R  S  L  E  E  
isotig125      2220 TTTTCCATGGTTCTGGTTTCGCTGTTAGGGAAAGGGGGACAGTGCAGGTCCTTGGAGGAG

isotig125       760 R  Q  G  H  D  R  V  S  G  G  E  S  C  Y  G  R  A  V  Y  W  
isotig125      2280 AGACAAGGACATGACCGGGTGTCTGGTGGTGAGTCCTGCTATGGAAGAGCTGTTTATTGG

isotig125       780 V  L  Q  G  D  R  D  S  R  E  D  Q  N  Q  A  S    796
isotig125      2340 GTACTTCAGGGTGACCGGGATTCAAGAGAAGACCAGAATCAGGCCTCA 2388
""",
        )
        alignment = protein_alignment.mapall(codon_alignments)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
                np.array([[   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,   36,   36,   63,   63,
                            213,  222,  240,  240,  330,  330,  330,  405,
                            405,  438,  450,  579,  579,  729,  729,  837,
                            837,  897,  912,  975,  987, 1020, 1026, 1074,
                           1077, 1107, 1107, 1116, 1119, 1182, 1188, 1302,
                           1302, 1392, 1398, 1455, 1458, 1470, 1695, 1695,
                           1824, 1824, 1887, 1887, 1896, 1902, 1956, 1980,
                           2022, 2025, 2043, 2046, 2061, 2106, 2187, 2220,
                           2232, 2253, 2271, 2331, 2334, 2352, 2364, 2373,
                           2382, 2424, 2463, 2484, 2517, 2541],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,   36,   36,   63,   63,
                            213,  222,  240,  240,  330,  330,  330,  405,
                            405,  438,  450,  579,  579,  729,  729,  837,
                            837,  897,  912,  975,  987, 1020, 1026, 1074,
                           1077, 1107, 1107, 1116, 1119, 1182, 1188, 1302,
                           1302, 1392, 1398, 1455, 1458, 1470, 1695, 1695,
                           1824, 1824, 1887, 1887, 1896, 1902, 1956, 1980,
                           2022, 2025, 2043, 2046, 2061, 2106, 2187, 2220,
                           2232, 2253, 2271, 2331, 2334, 2352, 2364, 2373,
                           2382, 2424, 2463, 2484, 2517, 2541],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,   36,   36,   63,   63,
                            213,  222,  240,  240,  330,  330,  330,  405,
                            405,  438,  450,  579,  579,  729,  729,  837,
                            837,  897,  912,  975,  987, 1020, 1026, 1074,
                           1077, 1107, 1107, 1116, 1119, 1182, 1188, 1302,
                           1302, 1392, 1398, 1455, 1458, 1470, 1695, 1695,
                           1824, 1824, 1887, 1887, 1896, 1902, 1956, 1980,
                           2022, 2025, 2043, 2046, 2061, 2106, 2187, 2220,
                           2232, 2253, 2271, 2331, 2334, 2352, 2364, 2373,
                           2382, 2424, 2463, 2484, 2517, 2541],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,   36,   36,   63,   63,
                            213,  222,  240,  240,  330,  330,  330,  405,
                            405,  438,  450,  579,  579,  729,  729,  837,
                            837,  897,  912,  975,  987, 1020, 1026, 1074,
                           1077, 1107, 1107, 1116, 1119, 1182, 1188, 1302,
                           1302, 1392, 1398, 1455, 1458, 1470, 1695, 1695,
                           1824, 1824, 1887, 1887, 1896, 1902, 1956, 1980,
                           2022, 2025, 2043, 2046, 2061, 2106, 2187, 2220,
                           2232, 2253, 2271, 2331, 2334, 2352, 2364, 2373,
                           2382, 2424, 2463, 2484, 2517, 2541],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,   75,
                             75,  108,  120,  249,  249,  399,  399,  507,
                            507,  567,  582,  645,  657,  690,  696,  744,
                            747,  777,  777,  786,  789,  852,  858,  972,
                            972, 1062, 1068, 1125, 1128, 1140, 1365, 1365,
                           1494, 1494, 1557, 1557, 1566, 1572, 1626, 1650,
                           1692, 1695, 1713, 1716, 1731, 1776, 1857, 1890,
                           1902, 1923, 1941, 2001, 2004, 2022, 2034, 2043,
                           2052, 2094, 2133, 2154, 2187, 2211],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,   75,
                             75,  108,  120,  249,  249,  399,  399,  507,
                            507,  567,  582,  645,  657,  690,  696,  744,
                            747,  777,  777,  786,  789,  852,  858,  972,
                            972, 1062, 1068, 1125, 1128, 1140, 1365, 1365,
                           1494, 1494, 1557, 1557, 1566, 1572, 1626, 1650,
                           1692, 1695, 1713, 1716, 1731, 1776, 1857, 1890,
                           1902, 1923, 1941, 2001, 2004, 2022, 2034, 2043,
                           2052, 2094, 2133, 2154, 2187, 2211],
                          [   0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,    0,
                              0,    0,    0,    0,    0,    0,    0,   75,
                             75,  108,  120,  249,  249,  399,  399,  507,
                            507,  567,  582,  645,  657,  690,  696,  744,
                            747,  777,  777,  786,  789,  852,  858,  972,
                            972, 1062, 1068, 1125, 1128, 1140, 1365, 1365,
                           1494, 1494, 1557, 1557, 1566, 1572, 1626, 1650,
                           1692, 1695, 1713, 1716, 1731, 1776, 1857, 1890,
                           1902, 1923, 1941, 2001, 2004, 2022, 2034, 2043,
                           2052, 2094, 2133, 2154, 2187, 2211],
                          [   0,   12,   21,  357,  378,  417,  444,  474,
                            516,  549,  564,  570,  606,  615,  642,  645,
                            795,  795,  813,  828,  918,  918,  999, 1074,
                           1077, 1110, 1110, 1239, 1251, 1401, 1428, 1536,
                           1560, 1620, 1620, 1683, 1683, 1716, 1716, 1764,
                           1764, 1794, 1809, 1818, 1818, 1881, 1881, 1995,
                           2001, 2091, 2091, 2148, 2148, 2148, 2373, 2373,
                           2502, 2532, 2595, 2619, 2628, 2628, 2682, 2706,
                           2706, 2709, 2727, 2727, 2727, 2772, 2772, 2805,
                           2817, 2817, 2835, 2895, 2898, 2916, 2928, 2937,
                           2946, 2946, 2985, 3006, 3039, 3063],
                          [   0,    0,    9,    9,   30,   30,   57,   57,
                             99,   99,  114,  114,  150,  159,  186,  189,
                            339,  339,  357,  372,  462,  465,  546,  621,
                            624,  657,  669,  798,  810,  960,  987, 1095,
                           1119, 1179, 1179, 1242, 1254, 1287, 1287, 1335,
                           1335, 1365, 1380, 1389, 1392, 1455, 1455, 1569,
                           1575, 1665, 1665, 1722, 1725, 1725, 1950, 1953,
                           2082, 2112, 2175, 2175, 2184, 2184, 2184, 2208,
                           2208, 2208, 2226, 2226, 2226, 2271, 2271, 2304,
                           2304, 2304, 2304, 2304, 2307, 2325, 2337, 2346,
                           2346, 2346, 2385, 2385, 2418, 2418],
                          [   0,    0,    9,    9,   30,   30,   57,   57,
                             99,   99,  114,  114,  150,  159,  186,  189,
                            339,  339,  357,  372,  462,  465,  546,  621,
                            624,  657,  669,  798,  810,  960,  987, 1095,
                           1119, 1179, 1179, 1242, 1254, 1287, 1287, 1335,
                           1335, 1365, 1380, 1389, 1392, 1455, 1455, 1569,
                           1575, 1665, 1665, 1722, 1725, 1725, 1950, 1953,
                           2082, 2112, 2175, 2175, 2184, 2184, 2184, 2208,
                           2208, 2208, 2226, 2229, 2229, 2229, 2229, 2262,
                           2274, 2274, 2274, 2334, 2337, 2337, 2349, 2349,
                           2349, 2349, 2388, 2388, 2388, 2388]])
                # fmt: on
            )
        )
        self.assertEqual(
            format(alignment, "clustal"),
            """\
ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
isotig12565                         ------------GCTAGGAGA-----------------------------
isotig12566                         ------------GCTAGGAGA-----------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         AAAAAAAAAAGAGTTGGCCCAAACCCCTGAGGGAGGCTGTCCTCGGCAGG
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         AGAGCAGACCTGAGGGAGGTTGGTGTGCCGGTCCTGGTTGTCCAGCCCTC
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CCGTCCCATCCCAGGGCTGGCTCACCAGAAAAAAGTACAGGCATCCCAGG
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CCCTCTGGGGCGGGAGCAGAGGGTCTCCTCTTGTCCGGGGGAAGACAAAA
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         ACAAAAACAAAAAACGAGTGTGTAGCCCTCACTGGAAGTCTTCTGGTGCT
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CTGGGGCCGTTTGCACTTGGGAGCCTGGCTTCTGGGCATGGTGGCCGGGC
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         TCCGCCGGGGGCTTCAGCCCTCGGCAAAGCGTTTCTAGAACAGAGTGGGT
isotig12565                         -------GGCCAGGCGGCCCTCGGGAGC----------------------
isotig12566                         -------GGCCAGGCGGCCCTCGGGAGC----------------------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         GGGAGCCGACTGAAGTCCCAGAACCGCGAACACTGACGCACAGGAAAGCC
isotig12565                         -----------------CCAGCTGCTCGCACCTGGAGCCAGCGC------
isotig12566                         -----------------CCAGCTGCTCGCACCTGGAGCCAGCGC------

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         TCGAGTGGGTGGAGAAATGCAAATCCCCCAGTGGCATGGCCTCAGCCGCT
isotig12565                         ------------------------AGCCCGGCCAGTCGGGCCTCAGCCCG
isotig12566                         ------------------------AGCCCGGCCAGTCGGGCCTCAGCCCG

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         GCGGACCCTGACGGCCGATTCTGACTTCGGACTTGAGCGGGACCAGCACC
isotig12565                         GGAGACAGTTACGCCC---------------------------------C
isotig12566                         GGAGACAGTTACGCCC---------------------------------C

ENSG00000166888:ENST0000030013      --------------------ATGTCTCTGTGGGGTCTGGTCTCCAAGATG
ENSG00000166888:ENST0000054387      --------------------ATGTCTCTGTGGGGTCTGGTCTCCAAGATG
ENSG00000166888:ENST0000055615      --------------------ATGTCTCTGTGGGGTCTGGTCTCCAAGATG
ENSG00000166888:ENST0000045407      --------------------ATGTCTCTGTGGGGTCTGGTCTCCAAGATG
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CGGACCCAGGGCGAGCCAGCATGTCTCAGTGGAATCAAGTCCAACAGTTA
isotig12565                         CTGATTGCGGCAGG------ATGGCCCAGTGGAACCAGCTGCAGCAGCTG
isotig12566                         CTGATTGCGGCAGG------ATGGCCCAGTGGAACCAGCTGCAGCAGCTG

ENSG00000166888:ENST0000030013      CCCCCA---------GAAAAAGTGCAGCGGCTCTATGTCGAC---TTTCC
ENSG00000166888:ENST0000054387      CCCCCA---------GAAAAAGTGCAGCGGCTCTATGTCGAC---TTTCC
ENSG00000166888:ENST0000055615      CCCCCA---------GAAAAAGTGCAGCGGCTCTATGTCGAC---TTTCC
ENSG00000166888:ENST0000045407      CCCCCA---------GAAAAAGTGCAGCGGCTCTATGTCGAC---TTTCC
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         GAAATCAAGTTTTTGGAGCAAGTTGATCAGTTCTATGATGACAACTTTCC
isotig12565                         GACACTCGGTACCTGGAGCAGCTGCACCAGCTGTACAGCGACAGCTTCCC
isotig12566                         GACACTCGGTACCTGGAGCAGCTGCACCAGCTGTACAGCGACAGCTTCCC

ENSG00000166888:ENST0000030013      CCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGG
ENSG00000166888:ENST0000054387      CCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGG
ENSG00000166888:ENST0000055615      CCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGG
ENSG00000166888:ENST0000045407      CCAACACCTGCGGCATCTTCTGGGTGACTGGCTGGAGAGCCAGCCCTGGG
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CATGGAAATCCGACATCTGCTGGCCCAGTGGATTGAGCATCAAGACTGGG
isotig12565                         CATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGATTGGG
isotig12566                         CATGGAGCTGCGGCAGTTCCTGGCACCTTGGATTGAGAGTCAAGATTGGG

ENSG00000166888:ENST0000030013      AGTTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTA
ENSG00000166888:ENST0000054387      AGTTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTA
ENSG00000166888:ENST0000055615      AGTTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTA
ENSG00000166888:ENST0000045407      AGTTCCTGGTCGGCTCCGACGCCTTCTGCTGCAACTTGGCTAGTGCCCTA
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         AGGTGGCCTCTAACAATGAAACTATGGCAACAATTCTTCTTCAAAACTTA
isotig12565                         CATATGCAGCCAGCAAAGAGTCACATGCCACACTGGTGTTTCATAATCTC
isotig12566                         CATATGCAGCCAGCAAAGAGTCACATGCCACACTGGTGTTTCATAATCTC

ENSG00000166888:ENST0000030013      CTTTCAGACACTGTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGA
ENSG00000166888:ENST0000054387      CTTTCAGACACTGTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGA
ENSG00000166888:ENST0000055615      CTTTCAGACACTGTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGA
ENSG00000166888:ENST0000045407      CTTTCAGACACTGTCCAGCACCTTCAGGCCTCGGTGGGAGAGCAGGGGGA
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         TTAATACAATTGGATGAACAGTTAGGTCGTGTTTCCAAAGAGAAA-----
isotig12565                         TTGGGTGAGATTGACCAGCAGTACAGCCGATTCCTGCAAGAGTCC-----
isotig12566                         TTGGGTGAGATTGACCAGCAGTACAGCCGATTCCTGCAAGAGTCC-----

ENSG00000166888:ENST0000030013      GGGGAGCACCATCTTGCAACAC---------------ATCAGCACCCTTG
ENSG00000166888:ENST0000054387      GGGGAGCACCATCTTGCAACAC---------------ATCAGCACCCTTG
ENSG00000166888:ENST0000055615      GGGGAGCACCATCTTGCAACAC---------------ATCAGCACCCTTG
ENSG00000166888:ENST0000045407      GGGGAGCACCATCTTGCAACAC---------------ATCAGCACCCTTG
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         ----AACCTGCTATTGATCCACAATCTAAAGAGAATTAGAAAAGTACTTC
isotig12565                         ----AACGTCCTCTATCAGCACAACCTTCGGAGGATCAAGCAGTTCCTAC
isotig12566                         ----AACGTCCTCTATCAGCACAACCTTCGGAGGATCAAGCAGTTCCTAC

ENSG00000166888:ENST0000030013      AGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAA
ENSG00000166888:ENST0000054387      AGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAA
ENSG00000166888:ENST0000055615      AGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAA
ENSG00000166888:ENST0000045407      AGAGCATATATCAGAGGGACCCCCTGAAGCTGGTGGCCACTTTCAGACAA
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         AGGGGAAGTTTCATGGAAATCCAATGCATGTAGCCGTGGTAATCTCAAAT
isotig12565                         AGAGCAGGTATCTTGAGAAGCCGATGGAAATCGCCCGCATCGTGGCCCGA
isotig12566                         AGAGCAGGTATCTTGAGAAGCCGATGGAAATCGCCCGCATCGTGGCCCGA

ENSG00000166888:ENST0000030013      ATACTTCAAGGAGAGAAAAAAGCTGTT-----------------------
ENSG00000166888:ENST0000054387      ATACTTCAAGGAGAGAAAAAAGCTGTT-----------------------
ENSG00000166888:ENST0000055615      ATACTTCAAGGAGAGAAAAAAGCTGTT-----------------------
ENSG00000166888:ENST0000045407      ATACTTCAAGGAGAGAAAAAAGCTGTT-----------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         TGTTTAAGGGAAGAGAGGAGAATACTG---GCTGCAGCGAACATGCCTAT
isotig12565                         TGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACTGCAGCCCA
isotig12566                         TGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACTGCAGCCCA

ENSG00000166888:ENST0000030013      --------------------------------------------------
ENSG00000166888:ENST0000054387      --------------------------------------------------
ENSG00000166888:ENST0000055615      --------------------------------------------------
ENSG00000166888:ENST0000045407      --------------------------------------------------
ENSG00000166888:ENST0000053891      --------------------------------------------------
ENSG00000166888:ENST0000053721      --------------------------------------------------
ENSG00000166888:ENST0000053520      --------------------------------------------------
isotig46679                         CCAGGGACCTCTGGAGAAATCCTTACAAAGTTCGTCGGTTTCAGAAAGAC
isotig12565                         GCAAGGAGGCCAGGCCAACCACCCAACAGCTGCTGTGGTGACGGAGAAAC
isotig12566                         GCAAGGAGGCCAGGCCAACCACCCAACAGCTGCTGTGGTGACGGAGAAAC

ENSG00000166888:ENST0000030013      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000054387      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000055615      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000045407      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000053891      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000053721      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
ENSG00000166888:ENST0000053520      -----------ATGGAACAGTTCCGCCACTTGCCAATGCCTTTCCACTGG
isotig46679                         AGAGAAATGTGGAACACAAAGTGGCTGCCATTAAAAACAGTGTGCAGATG
isotig12565                         AGCAGATGCTGGAGCAGCATCTTCAGGATGTCCGGAAACGTGTGCAGGAT
isotig12566                         AGCAGATGCTGGAGCAGCATCTTCAGGATGTCCGGAAACGTGTGCAGGAT

ENSG00000166888:ENST0000030013      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000054387      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000055615      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000045407      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000053891      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000053721      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
ENSG00000166888:ENST0000053520      AAGCAGGAAGAACTCAAGTTTAAGACAGGCTTGCGG---AGGCTGCAGCA
isotig46679                         ACAGAACAAGACACCAAATACTTGGAAGATCTGCAAGATGAATTTGACTA
isotig12565                         CTAGAACAGAAAATGAAAGTGGTAGAGAATCTCCAGGATGACTTTGATTT
isotig12566                         CTAGAACAGAAAATGAAAGTGGTAGAGAATCTCCAGGATGACTTTGATTT

ENSG00000166888:ENST0000030013      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000054387      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000055615      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000045407      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000053891      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000053721      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
ENSG00000166888:ENST0000053520      CCGAGTAGGGGAGATCCACCTTCTCCGAGAAGCCCTGCAGAAGGGGGCTG
isotig46679                         CAGGTATAAAACAATTCAGACA------------ATGGACCAGGGTGACA
isotig12565                         CAACTATAAAACCCTCAAGAGTCAAGGAGACATGCAGGATCTGAATGGAA
isotig12566                         CAACTATAAAACCCTCAAGAGTCAAGGAGACATGCAGGATCTGAATGGAA

ENSG00000166888:ENST0000030013      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000054387      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000055615      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000045407      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000053891      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000053721      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
ENSG00000166888:ENST0000053520      AGGCTGGCCAAGTGTCTCTGCACAGCTTGATAGAAACTCCTGCTAATGGG
isotig46679                         AGAATAGCATCCTAATGAACCAGGAGGTTTTGACACTCCAAGAAATGCTT
isotig12565                         ACAACCAGTCTGTGACCAGGCAGAAGATGCAGCAGCTGGAACAGATGCTC
isotig12566                         ACAACCAGTCTGTGACCAGGCAGAAGATGCAGCAGCTGGAACAGATGCTC

ENSG00000166888:ENST0000030013      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000054387      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000055615      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000045407      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000053891      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000053721      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
ENSG00000166888:ENST0000053520      ACTGGGCCAAGTGAGGCCCTGGCCATGCTACTGCAGGAGACCACTGGAGA
isotig46679                         AATAGCCTGGACTTCAAGAGAAAGGAAGCACTCACTAAGATGACACAGAT
isotig12565                         ACGGCGCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCT
isotig12566                         ACGGCGCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCT

ENSG00000166888:ENST0000030013      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000054387      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000055615      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000045407      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000053891      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000053721      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
ENSG00000166888:ENST0000053520      GCTAGAGGCAGCC------------AAAGCCCTAGTGCTGAAGAGGATCC
isotig46679                         AGTGAACGAGTCGGACCTGCTGATGAGCAGCATGCTCATAGAAGAGCTGC
isotig12565                         TTTGTCGGCAATGGAGTACGTGCAGAAAACACTCACAGACGAGGAGCTGG
isotig12566                         TTTGTCGGCAATGGAGTACGTGCAGAAAACACTCACAGACGAGGAGCTGG

ENSG00000166888:ENST0000030013      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000054387      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000055615      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000045407      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000053891      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000053721      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
ENSG00000166888:ENST0000053520      AGATTTGGAAACGGCAGCAGCAGCTGGCAGGGAATGGCGCACCGTTTGAG
isotig46679                         AGGACTGGAAGAGGAGGCAGCAGATCGCCTGCATCGGTGGCCCACTCCAC
isotig12565                         CTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATTGGAGGCCCTCCCAAC
isotig12566                         CTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATTGGAGGCCCTCCCAAC

ENSG00000166888:ENST0000030013      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000054387      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000055615      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000045407      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000053891      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000053721      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
ENSG00000166888:ENST0000053520      GAGAGCCTGGCCCCACTCCAGGAGAGGTGTGAAAGCCTGGTGGACATTTA
isotig46679                         AACGGGCTGGACCAGCTTCAGAACTGCTTTACCCTGTTGGCAGAAAGTCT
isotig12565                         ATCTGCCTGGATCGCCTGGAAAACTGGATAACTTCGTTAGCAGAATCTCA
isotig12566                         ATCTGCCTGGATCGCCTGGAAAACTGGATAACTTCGTTAGCAGAATCTCA

ENSG00000166888:ENST0000030013      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000054387      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000055615      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000045407      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000053891      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000053721      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
ENSG00000166888:ENST0000053520      TTCCCAGCTACAGCAGGAGGTAGGG-------------------------
isotig46679                         TTTCCAACTCAGACGACAGCTGGAGAAATTAGAGGAGCAGTCTTCCAAGA
isotig12565                         ACTTCAGACCCGCCAACAAATTAAGAAACTGGAGGAGCTACAGCAGAAGG
isotig12566                         ACTTCAGACCCGCCAACAAATTAAGAAACTGGAGGAGCTACAGCAGAAGG

ENSG00000166888:ENST0000030013      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000054387      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000055615      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000045407      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000053891      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000053721      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
ENSG00000166888:ENST0000053520      --GCGGCTGGTGGGGAGCTTGAGCCCAAGACCCGGGCATCGCTGACTGGC
isotig46679                         TGACTTACGAAGGAGACCCCATCCCCACGCAGAGAGCACACCTGCTGGAG
isotig12565                         TGTCCTACAAGGGGGACCCCATTGTGCAGCACCGGCCGATGCTGGAGGAG
isotig12566                         TGTCCTACAAGGGGGACCCCATTGTGCAGCACCGGCCGATGCTGGAGGAG

ENSG00000166888:ENST0000030013      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000054387      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000055615      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000045407      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000053891      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000053721      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
ENSG00000166888:ENST0000053520      CGGCTGGATGAAGTCCTGAGAACCCTCGTCACCAGTTGCTTCCTGGTGGA
isotig46679                         AGAGCCACCTTCCTGATCTACAACCTTTTCAAGAACTCATTTGTGGTTGA
isotig12565                         CGGATCGTGGAGCTGTTCAGAAACTTGATGAAGAGTGCCTTCGTGGTGGA
isotig12566                         CGGATCGTGGAGCTGTTCAGAAACTTGATGAAGAGTGCCTTCGTGGTGGA

ENSG00000166888:ENST0000030013      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000054387      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000055615      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000045407      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000053891      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000053721      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
ENSG00000166888:ENST0000053520      GAAGCAGCCC------------------------CCCCAGGTACTGAAGA
isotig46679                         GCGACAGCCCTGCATGCCAACACACCCTCAGAGGCCGCTGGTACTCAAAA
isotig12565                         GCGACAGCCCTGCATGCCGATGCACCCCGACCGGCCCTTGGTCATCAAGA
isotig12566                         GCGACAGCCCTGCATGCCGATGCACCCCGACCGGCCCTTGGTCATCAAGA

ENSG00000166888:ENST0000030013      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000054387      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000055615      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000045407      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000053891      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000053721      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
ENSG00000166888:ENST0000053520      CTCAGACCAAGTTCCAGGCTGGAGTTCGATTCCTGTTGGGCTTGAGGTTC
isotig46679                         CCCTCATTCAGTTCACCGCGAAACTGAGACTACTAATAAAATTG------
isotig12565                         CTGGTGTCCAGTTCACTACTAAAGTCAGGTTGTTGGTCAAGTTT------
isotig12566                         CTGGTGTCCAGTTCACTACTAAAGTCAGGTTGTTGGTCAAGTTT------

ENSG00000166888:ENST0000030013      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000054387      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000055615      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000045407      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000053891      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000053721      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
ENSG00000166888:ENST0000053520      CTGGGGGCCCCAGCCAAGCCTCCGCTGGTCAGGGCCGACATGGTGACAGA
isotig46679                         ---------CCGGAACTCAACTATCAGGTGAAAGTAAAGGCATCGATCGA
isotig12565                         ---------CCCGAGTTGAATTATCAGCTTAAAATTAAAGTGTGCATTGA
isotig12566                         ---------CCCGAGTTGAATTATCAGCTTAAAATTAAAGTGTGCATTGA

ENSG00000166888:ENST0000030013      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000054387      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000055615      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000045407      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000053891      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000053721      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
ENSG00000166888:ENST0000053520      GAAGCAGGCGCGGGAGCTGAGTGTGCCTCAGGGTCCTGGGGCTGGAGCAG
isotig46679                         CAAGAATGTTTCAACGCTAAGC------------AATAGAAGATTTGTGC
isotig12565                         CAAAGATTCTGGGGACGTTGCTGCTCTCAGAGGATCTCGGAAATTTAACA
isotig12566                         CAAAGATTCTGGGGACGTTGCTGCTCTCAGAGGATCTCGGAAATTTAACA

ENSG00000166888:ENST0000030013      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000054387      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000055615      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000045407      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000053891      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000053721      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
ENSG00000166888:ENST0000053520      AAAGCACTGGAGAAATCATCAACAACACTGTGCCCTTGGAGAACAGCATT
isotig46679                         TTTGTGGAACTCAAGTC------AAAGCCATGTCCATCGAGGAATCCTCC
isotig12565                         TTCTGGGCACAAACACG------AAGGTGATGAACATGGAAGAATCCAAC
isotig12566                         TTCTGGGCACAAACACG------AAGGTGATGAACATGGAAGAATCCAAC

ENSG00000166888:ENST0000030013      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000054387      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000055615      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000045407      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000053891      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000053721      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
ENSG00000166888:ENST0000053520      CCTGGGAACTGCTGCTCTGCCCTGTTCAAGAACCTGCTTCTCAAGAAGAT
isotig46679                         AATGGGAGCCTCTCAGTAGAA---TTTAGACATTTGCAACCGAAGGAAAT
isotig12565                         AACGGCAGCCTGTCTGCGGAG---TTCAAGCACTTGACCCTGAGGGAGCA
isotig12566                         AACGGCAGCCTGTCTGCGGAG---TTCAAGCACTTGACCCTGAGGGAGCA

ENSG00000166888:ENST0000030013      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000054387      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000055615      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000045407      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000053891      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000053721      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
ENSG00000166888:ENST0000053520      CAAG---------------CGGTGTGAGCGGAAGGGCACTGAGTCTGTCA
isotig46679                         GAAATCCAGTGCCGGAAGTAAAGGAAAT---GAGGGCTGCCACATGGTGA
isotig12565                         GAGATGTGGGAATGGAGGCCGTGCCAATTGTGATGCCTCCTTGATTGTGA
isotig12566                         GAGATGTGGGAATGGAGGCCGTGCCAATTGTGATGCCTCCTTGATTGTGA

ENSG00000166888:ENST0000030013      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000054387      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000055615      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000045407      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000053891      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000053721      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
ENSG00000166888:ENST0000053520      CAGAGGAGAAGTGCGCTGTGCTCTTCTCTGCCAGCTTCACACTTGGCCCC
isotig46679                         CGGAAGAGCTGCATTCCATAGCCTTTGAGACCCAGATCTGCCTC------
isotig12565                         CCGAGGAGCTGCATCTGATCACCTTCGAGACTGAGGTGTACCAC------
isotig12566                         CCGAGGAGCTGCATCTGATCACCTTCGAGACTGAGGTGTACCAC------

ENSG00000166888:ENST0000030013      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000054387      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000055615      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000045407      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000053891      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000053721      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
ENSG00000166888:ENST0000053520      GGCAAACTCCCCATCCAGCTCCAGGCCCTGTCTCTGCCCCTGGTGGTCAT
isotig46679                         TATGGCCTCACCATCGACTTGGAGACAAGCTCATTACCTGTGGTGATGAT
isotig12565                         CAAGGCCTCAAGATTGACCTGGAGACCCATTCTTTGCCAGTTGTGGTGAT
isotig12566                         CAAGGCCTCAAGATTGACCTGGAGACCCATTCTTTGCCAGTTGTGGTGAT

ENSG00000166888:ENST0000030013      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000054387      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000055615      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000045407      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000053891      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000053721      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
ENSG00000166888:ENST0000053520      CGTCCATGGCAACCAAGACAACAATGCCAAAGCCACTATCCTGTGGGACA
isotig46679                         TTCTAATGTCAGCCAACTGCCTAATGCTTGGGCATCCATCATTTGGTACA
isotig12565                         CTCCAACATCTGTCAGATGCCAAATGCCTGGGCATCCATCCTGTGGTATA
isotig12566                         CTCCAACATCTGTCAGATGCCAAATGCCTGGGCATCCATCCTGTGGTATA

ENSG00000166888:ENST0000030013      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000054387      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000055615      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000045407      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000053891      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000053721      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
ENSG00000166888:ENST0000053520      ATGCCTTCTCTGAG------ATGGACCGCGTGCCCTTTGTGGTGGCTGAG
isotig46679                         ATGTGTCAACCAACGATTGCCAGAACTTGGTTTTCTTTAATAATCCTCCG
isotig12565                         ACATGCTGACCAACAACCCCAAGAACGTGAACTTCTTCACCAAGCCACCA
isotig12566                         ACATGCTGACCAACAACCCCAAGAACGTGAACTTCTTCACCAAGCCACCA

ENSG00000166888:ENST0000030013      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000054387      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000055615      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000045407      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000053891      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000053721      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
ENSG00000166888:ENST0000053520      CGGGTGCCCTGGGAGAAGATGTGTGAAACTCTGAACCTGAAGTTCATGGC
isotig46679                         CCTGTCACTTTGAGTCAACTCCTGGAAGTGATGAGCTGGCAGTTTTCATC
isotig12565                         ATCGGAACCTGGGACCAGGTGGCCGAGGTGCTCAGCTGGCAGTTCTCATC
isotig12566                         ATCGGAACCTGGGACCAGGTGGCCGAGGTGCTCAGCTGGCAGTTCTCATC

ENSG00000166888:ENST0000030013      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000054387      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000055615      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000045407      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000053891      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000053721      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
ENSG00000166888:ENST0000053520      TGAGGTGGGGACCAACCGGGGGCTGCTCCCAGAGCACTTCCTCTTCCTGG
isotig46679                         CTATGTTGGT------CGTGGCCTTAATTCAGACCAGCTCAACATGCTGG
isotig12565                         CACCACAAAG------CGAGGGCTGAGCATCGAGCAGCTGACTACGCTGG
isotig12566                         CACCACAAAG------CGAGGGCTGAGCATCGAGCAGCTGACTACGCTGG

ENSG00000166888:ENST0000030013      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000054387      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000055615      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000045407      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000053891      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000053721      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
ENSG00000166888:ENST0000053520      CCCAGAAGATCTTCAATGACAACAGCCTCAGTATGGAGGCCTTCCAGCAC
isotig46679                         CAGAGAAGCTCACAGTTCAGTCT---------------AACTACAGCGAT
isotig12565                         CCGAGAAGCTCCTAGGACCTGGTGTC------------AACTACTCCGGG
isotig12566                         CCGAGAAGCTCCTAGGACCTGGTGTC------------AACTACTCCGGG

ENSG00000166888:ENST0000030013      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000054387      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000055615      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000045407      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000053891      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000053721      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
ENSG00000166888:ENST0000053520      CGTTCTGTGTCCTGGTCGCAGTTCAACAAGGAGATCCTGCTGGGCCGTGG
isotig46679                         GGTCACCTCACCTGGGCCAAGTTCTGCAAGGAACACTTGCCTGGCAAACC
isotig12565                         TGTCAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGG
isotig12566                         TGTCAGATCACATGGGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGG

ENSG00000166888:ENST0000030013      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000054387      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000055615      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000045407      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000053891      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000053721      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
ENSG00000166888:ENST0000053520      CTTCACCTTTTGGCAGTGGTTTGATGGTGTCCTGGACCTCACCAAACGCT
isotig46679                         ATTTACCTTCTGGACCTGGCTTGAAGCAATATTGGACCTAATTAAAAAAC
isotig12565                         CTTCTCCTTCTGGGTGTGGCTAGACAATATCATTGACCTTGTGAAAAAGT
isotig12566                         CTTCTCCTTCTGGGTGTGGCTAGACAATATCATTGACCTTGTGAAAAAGT

ENSG00000166888:ENST0000030013      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000054387      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000055615      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000045407      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000053891      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000053721      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
ENSG00000166888:ENST0000053520      GTCTCCGGAGCTACTGGTCTGACCGGCTGATCATTGGCTTCATCAGCAAA
isotig46679                         ACATTCTTCCCCTCTGGATTGATGGGTACATCATGGGCTTCGTGAGCAAA
isotig12565                         ATATCTTGGCCCTCTGGAATGAAGGGTACATCATGGGCTTCATTAGCAAG
isotig12566                         ATATCTTGGCCCTCTGGAATGAAGGGTACATCATGGGCTTCATTAGCAAG

ENSG00000166888:ENST0000030013      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000054387      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000055615      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000045407      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000053891      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000053721      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
ENSG00000166888:ENST0000053520      CAGTACGTTACTAGCCTTCTTCTCAATGAGCCCGACGGAACCTTTCTCCT
isotig46679                         GAGAAGGAGAGGTTTCTGCTCAAGGATAAAATGCCCGGGACATTTTTGTT
isotig12565                         GAGCGGGAGCGGGCGATCCTGAGCACGAAACCCCCGGGCACCTTCCTGCT
isotig12566                         GAGCGGGAGCGGGCGATCCTGAGCACGAAACCCCCGGGCACCTTCCTGCT

ENSG00000166888:ENST0000030013      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000054387      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000055615      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000045407      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000053891      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000053721      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
ENSG00000166888:ENST0000053520      CCGCTTCAGCGAC---TCAGAGATTGGGGGCATCACCATTGCCCATGTCA
isotig46679                         ACGATTCAGTGAG---AGCCATCTCGGAGGGATCACCTTCACCTGGGTGG
isotig12565                         GAGATTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACTTGGGTGG
isotig12566                         GAGATTCAGCGAGAGCAGCAAAGAAGGAGGGGTCACTTTCACTTGGGTGG

ENSG00000166888:ENST0000030013      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000054387      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000055615      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000045407      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000053891      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000053721      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
ENSG00000166888:ENST0000053520      TCCGGGGCCAGGATGGCTCTCCACAGATAGAGAACATCCAGCCATTCTCT
isotig46679                         ACCACTCTGAAAACGGAGAAGTGAGATTCCACTCCGTAGAACCCTACAAC
isotig12565                         AAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCGTACACC
isotig12566                         AAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCGTACACC

ENSG00000166888:ENST0000030013      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000054387      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000055615      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000045407      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000053891      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000053721      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
ENSG00000166888:ENST0000053520      GCCAAAGACCTGTCCATTCGCTCACTGGGGGACCGAATCCGGGAT-----
isotig46679                         AAAGGGCGTCTGTCGGCCCTGCCATTTGCTGACATCCTGCGGGACTACAA
isotig12565                         AAGCAGCAGCTGAACAACATGTCCTTTGCTGAAATCATCATGGGCTACAA
isotig12566                         AAGCAGCAGCTGAACAACATGTCCTTTGCTGAAATCATCATGGGCTACAA

ENSG00000166888:ENST0000030013      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000054387      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000055615      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000045407      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000053891      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000053721      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
ENSG00000166888:ENST0000053520      -------------------------CTTGCTCAGCTCAAAAATCTCTATC
isotig46679                         GGTCATCATGGCTGAGAACATTCCCGAGAACCCTCTCAAGTACCTCTACC
isotig12565                         GATCATGGATGCCACCAACATCCTGGTGTCCCCATTGGTCTACCTCTACC
isotig12566                         GATCATGGATGCCACCAACATCCTGGTGTCCCCATTGGTCTACCTCTACC

ENSG00000166888:ENST0000030013      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000054387      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000055615      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000045407      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000053891      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000053721      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
ENSG00000166888:ENST0000053520      CCAAGAAGCCCAAGGATGAGGCTTTCCGGAGCCACTAC------------
isotig46679                         CCGACATCCCCAAAGACAAAGCCTTCGGTAAACACTACAGCTCCCAGCCT
isotig12565                         CTGACATTCCCAAGGAGGAGGCGTTCGGGAAGTACTGT------------
isotig12566                         CTGACATTCCCAAGGAGGAGGCGTTCGGGAAGTACTGT------------

ENSG00000166888:ENST0000030013      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000054387      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000055615      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000045407      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000053891      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000053721      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
ENSG00000166888:ENST0000053520      ------------AAGCCTGAACAGATGGGTAAGGATGGCAGGGGTTATGT
isotig46679                         TGCGAAGTTTCAAGGCCAACA------GAACGGGGAGACAAAGGTTATGT
isotig12565                         ------------CGACCAGAG-----------------------------
isotig12566                         ------------CGACCAGAG-----------------------------

ENSG00000166888:ENST0000030013      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000054387      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000055615      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000045407      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000053891      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000053721      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
ENSG00000166888:ENST0000053520      CCCAGCTACCATCAAGATGACCGTGGAAAGGGACCAACCACTTCCTACCC
isotig46679                         TCCTTCAGTTTTTATCCCTATTTCAACAATCCGCAGCGACGCCATGGAGC
isotig12565                         -------------------------------AGCCAGGAGCATCCTGAAG
isotig12566                         -------------------------------AGCCAGGAGCATCCTGAAG

ENSG00000166888:ENST0000030013      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000054387      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000055615      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000045407      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000053891      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000053721      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
ENSG00000166888:ENST0000053520      CAGAGCTCCAGATGCCTACCATGGTGCCTTCTTATGACCTTGGAATGGCC
isotig46679                         CGCAG------------------------------------------TCT
isotig12565                         CTGAC---------------------------------------------
isotig12566                         CTGAC---------------------------------------------

ENSG00000166888:ENST0000030013      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000054387      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000055615      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000045407      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000053891      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000053721      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
ENSG00000166888:ENST0000053520      CCTGATTCCTCCATGAGCATGCAGCTTGGCCCAGATATGGTGCCCCAGGT
isotig46679                         CCTTCAGACCTTCTCCCC------------------ATGTCTCCGAGTGT
isotig12565                         CCCGGTAGTGCCGCCCCT------------------TACCTGAAGACCAA
isotig12566                         CCCGGTAGTTGTTTTTCCATG-----------------------------

ENSG00000166888:ENST0000030013      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000054387      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000055615      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000045407      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000053891      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000053721      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
ENSG00000166888:ENST0000053520      GTACCCACCACACTCTCACTCCATCCCCCCGTATCAAGGCCTCTCCCCAG
isotig46679                         ATACGCTGTGCTGAGAGAAAACCTGAGCCCT-------------------
isotig12565                         GTTCATCTGTGTGACACCAACGACCTGCAGC-------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000054387      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000055615      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000045407      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000053891      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000053721      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
ENSG00000166888:ENST0000053520      AAGAATCAGTCAACGTGTTGTCAGCCTTCCAGGAGCCTCACCTGCAGATG
isotig46679                         --------------------------------------------------
isotig12565                         --------------------------------------------------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000054387      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000055615      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000045407      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000053891      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000053721      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
ENSG00000166888:ENST0000053520      CCCCCCAGCCTGGGCCAGATGAGCCTGCCCTTTGACCAGCCTCACCCCCA
isotig46679                         ------------ACCACAATTGAAACAGCAATGAAGTCTCCATATTCTGA
isotig12565                         ------------AATACCATTGACCTGCCGATGTCCCCCCCGCAC-----
isotig12566                         ------------GTTCTGGTTTCGCTGTTAGGGAAAGGGGGACAGTGCAG

ENSG00000166888:ENST0000030013      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000054387      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000055615      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000045407      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000053891      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000053721      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
ENSG00000166888:ENST0000053520      GGGCCTGCTGCCGTGCCAGCCTCAGGAGCATGCTGTGTCCAGCCCTGACC
isotig46679                         GCGGTAC---------------------AAAGCGACTCTTCAAGGAAGAG
isotig12565                         --------------------------------------------------
isotig12566                         GTCCTTG---------------------------------------GAGG

ENSG00000166888:ENST0000030013      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000054387      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000055615      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000045407      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000053891      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000053721      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
ENSG00000166888:ENST0000053520      CCCTGCTCTGCTCAGATGTGACCATGGTGGAAGACAGCTGCCTGAGCCAG
isotig46679                         AGCAGATGAAAACGGAGACTGCTCTTTGCCAAAGTCCACAATTCATTTCT
isotig12565                         --------------------------------------------------
isotig12566                         AGAGACAAGGACATGACCGGGTGTCTGGTGGTGAGTCCTGCTATGGAAGA

ENSG00000166888:ENST0000030013      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000054387      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000055615      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000045407      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000053891      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000053721      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
ENSG00000166888:ENST0000053520      CCAGTGACAGCGTTTCCTCAGGGCACTTGGATTGGTGAAGACATATTCCC
isotig46679                         TCAGCTTTGATACTGGTTTCTAGAAAATGGCACAAATCCGAAGCTTTCCT
isotig12565                         ------TTTAGATTCATTGATGCAGTTTGGAAACGGAGGTGCGCCCTC--
isotig12566                         GCTGTTTAT------------------TGGGTACTTCAG-----------

ENSG00000166888:ENST0000030013      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000054387      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000055615      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000045407      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000053891      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000053721      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
ENSG00000166888:ENST0000053520      TCCTCTGCTGCCTCCCACTGAACAGGACCTCACTAAGCTTCTCCTGGAGG
isotig46679                         CTCACTA------------------------------------------G
isotig12565                         -------------------------------------------------G
isotig12566                         -------------------------------------------------G

ENSG00000166888:ENST0000030013      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000054387      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000055615      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000045407      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000053891      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000053721      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
ENSG00000166888:ENST0000053520      GGCAAGGGGAGTCGGGGGGAGGGTCCTTGGGGGCACAGCCCCTCCTGCAG
isotig46679                         GTGACATTCCCCAACTGGGAGTGCTGCTGAAATGCAAACCAAAGCTTCAG
isotig12565                         GCAGGAGGGCAGTTGTCACTCACGTTCATGGATCTGAC------------
isotig12566                         GTGACCGGGATTCAAGAGAAGACCAGAATCAGGCCTCA------------

ENSG00000166888:ENST0000030013      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000054387      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000055615      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000045407      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000053891      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000053721      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
ENSG00000166888:ENST0000053520      CCCTCCCACTATGGGCAATCTGGGATCTCAATGTCCCACATGGACCTAAG
isotig46679                         ATAAACACGCAGGAAAAGACAGCTTCGAGAAACCTATGTTCGCAATATAA
isotig12565                         ---------TTCGGAGTGCGCTACCTCCCCCATGTGAGGAGC--------
isotig12566                         --------------------------------------------------

ENSG00000166888:ENST0000030013      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000054387      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000055615      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000045407      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000053891      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000053721      GGCCAACCCCAGTTGG
ENSG00000166888:ENST0000053520      GGCCAACCCCAGTTGG
isotig46679                         CAGAAGGCTGCTTTGC
isotig12565                         ----------------
isotig12566                         ----------------


""",
        )

    def test5(self):
        aligner = CodonAligner()
        # aligner.frameshift_score = -10.0
        nucleotide_records = SeqIO.parse("codonalign/nucl5.fa", "fasta")
        protein_alignment = Align.read("codonalign/pro5.aln", "clustal")
        self.assertEqual(len(protein_alignment.sequences), 3)
        codon_alignments = []
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[0]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 183], [0, 549]]))
        )
        self.assertEqual(
            str(alignment),
            """\
isotig697         0 R  G  D  Q  R  S  N  F  Q  L  S  P  S  T  M  Q  I  S  T  G  
isotig697         0 TGAGGCGATCAACGCAGCAACTTCCAGCTGTCTCCCTCCACCATGCAGATCTCCACAGGG

isotig697        20 L  L  C  L  L  L  V  A  T  G  F  T  S  Q  V  L  A  H  P  G  
isotig697        60 CTTCTGTGCcTGCTGCTTGTGGCCACTGGCTTCACTTCCCAGGTGCTGGCTCACCCAGGC

isotig697        40 S  I  P  S  T  Y  C  F  V  M  T  S  K  K  I  P  K  S  L  L  
isotig697       120 TCTATCCCATCTACCTaCTGCTTTGTTATGACCAGTAAGAaGATCCCCAAATCACTACTG

isotig697        60 K  S  Y  K  R  I  S  N  S  R  C  T  L  K  A  I  L  F  K  T  
isotig697       180 AaGAGCTACAAAaGAATCTCCAACAGCAGATGCACCcTGAAAGCCATACTCTTCAAGACC

isotig697        80 K  S  G  K  E  I  C  A  D  P  K  K  K  W  V  Q  D  A  T  K  
isotig697       240 AAGTCGGGCAAAGAGATCTGTGCTGACCCCAAGAAGAAGTGGGTCcAGGATGCCACAAAG

isotig697       100 H  L  D  Q  I  L  Q  T  P  K  P  T  I  P  S  F  E  T  H  P  
isotig697       300 CACCTGGACCAAATCCTTCAAACTCCAAAACCGACAATCCCCTCTTTTGAGACTCACCCA

isotig697       120 E  T  K  K  C  F  I  H  S  P  F  L  R  R  A  P  R  S  T  Q  
isotig697       360 GAGACTAAGAAATGCTTCATTCATTCTCCATTCCTAAGACGTGCTCCAAGGTCAACTCAG

isotig697       140 H  H  S  P  R  T  W  L  H  L  V  M  D  R  T  E  S  H  Y  V  
isotig697       420 CACCATTCCCCAAGGACTTGGCTTCATTTAGTTATGGATAGAACTGAAAGTCATTATGTT

isotig697       160 Q  N  K  P  D  L  K  R  L  C  N  F  L  N  M  Q  N  L  K  R  
isotig697       480 CAGAATAAGCCAGACTTGAAGAGGTTGTGTAATTTCTTGAATATGCAAAATCTTAAAAGG

isotig697       180 G  A  C   183
isotig697       540 GGGGCATGC 549
""",
        )
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[1]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 65], [0, 195]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  K  V  S  A  A  L  L  C  L  L  L  I  A  A  T  F  I  P  Q  
ENSG00000         0 ATGAAAGTCTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCCAA

ENSG00000        20 G  L  A  Q  P  D  A  I  N  A  P  V  T  C  C  Y  N  F  T  N  
ENSG00000        60 GGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTATAACTTCACCAAT

ENSG00000        40 R  K  I  S  V  Q  R  L  A  S  Y  R  R  I  T  S  S  K  C  P  
ENSG00000       120 AGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAGAATCACCAGCAGCAAGTGTCCC

ENSG00000        60 K  E  A  V  M    65
ENSG00000       180 AAAGAAGCTGTGATG 195
""",
        )
        nucleotide_record = next(nucleotide_records)
        protein_record = protein_alignment.sequences[2]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 99], [9, 306]]))
        )
        self.assertEqual(
            str(alignment),
            """\
ENSG00000         0 M  K  V  S  A  A  L  L  C  L  L  L  I  A  A  T  F  I  P  Q  
ENSG00000         9 ATGAAAGTCTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCCAA

ENSG00000        20 G  L  A  Q  P  D  A  I  N  A  P  V  T  C  C  Y  N  F  T  N  
ENSG00000        69 GGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTATAACTTCACCAAT

ENSG00000        40 R  K  I  S  V  Q  R  L  A  S  Y  R  R  I  T  S  S  K  C  P  
ENSG00000       129 AGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAGAATCACCAGCAGCAAGTGTCCC

ENSG00000        60 K  E  A  V  I  F  K  T  I  V  A  K  E  I  C  A  D  P  K  Q  
ENSG00000       189 AAAGAAGCTGTGATCTTCAAGACCATTGTGGCCAAGGAGATCTGTGCTGACCCCAAGCAG

ENSG00000        80 K  W  V  Q  D  S  M  D  H  L  D  K  Q  T  Q  T  P  K  T  
ENSG00000       249 AAGTGGGTTCAGGATTCCATGGACCACCTGGACAAGCAAACCCAAACTCCGAAGACT

ENSG00000        99
ENSG00000       306
""",
        )
        alignment = protein_alignment.mapall(codon_alignments)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
                np.array([[0, 42, 126, 126, 231, 333, 549],
                          [0,  0,  84,  90, 195, 195, 195],
                          [9,  9,  93,  99, 204, 306, 306]])
                # fmt: on
            )
        )
        self.assertEqual(
            format(alignment, "clustal"),
            """\
isotig69710                         TGAGGCGATCAACGCAGCAACTTCCAGCTGTCTCCCTCCACCATGCAGAT
ENSG00000108691:ENST0000058090      ------------------------------------------ATGAAAGT
ENSG00000108691:ENST0000022583      ------------------------------------------ATGAAAGT

isotig69710                         CTCCACAGGGCTTCTGTGCcTGCTGCTTGTGGCCACTGGCTTCACTTCCC
ENSG00000108691:ENST0000058090      CTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCC
ENSG00000108691:ENST0000022583      CTCTGCCGCCCTTCTGTGCCTGCTGCTCATAGCAGCCACCTTCATTCCCC

isotig69710                         AGGTGCTGGCTCACCCAGGCTCTATC------CCATCTACCTaCTGCTTT
ENSG00000108691:ENST0000058090      AAGGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTAT
ENSG00000108691:ENST0000022583      AAGGGCTCGCTCAGCCAGATGCAATCAATGCCCCAGTCACCTGCTGTTAT

isotig69710                         GTTATGACCAGTAAGAaGATCCCCAAATCACTACTGAaGAGCTACAAAaG
ENSG00000108691:ENST0000058090      AACTTCACCAATAGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAG
ENSG00000108691:ENST0000022583      AACTTCACCAATAGGAAGATCTCAGTGCAGAGGCTCGCGAGCTATAGAAG

isotig69710                         AATCTCCAACAGCAGATGCACCcTGAAAGCCATACTCTTCAAGACCAAGT
ENSG00000108691:ENST0000058090      AATCACCAGCAGCAAGTGTCCCAAAGAAGCTGTGATG-------------
ENSG00000108691:ENST0000022583      AATCACCAGCAGCAAGTGTCCCAAAGAAGCTGTGATCTTCAAGACCATTG

isotig69710                         CGGGCAAAGAGATCTGTGCTGACCCCAAGAAGAAGTGGGTCcAGGATGCC
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      TGGCCAAGGAGATCTGTGCTGACCCCAAGCAGAAGTGGGTTCAGGATTCC

isotig69710                         ACAAAGCACCTGGACCAAATCCTTCAAACTCCAAAACCGACAATCCCCTC
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      ATGGACCACCTGGACAAGCAAACCCAAACTCCGAAGACT-----------

isotig69710                         TTTTGAGACTCACCCAGAGACTAAGAAATGCTTCATTCATTCTCCATTCC
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      --------------------------------------------------

isotig69710                         TAAGACGTGCTCCAAGGTCAACTCAGCACCATTCCCCAAGGACTTGGCTT
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      --------------------------------------------------

isotig69710                         CATTTAGTTATGGATAGAACTGAAAGTCATTATGTTCAGAATAAGCCAGA
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      --------------------------------------------------

isotig69710                         CTTGAAGAGGTTGTGTAATTTCTTGAATATGCAAAATCTTAAAAGGGGGG
ENSG00000108691:ENST0000058090      --------------------------------------------------
ENSG00000108691:ENST0000022583      --------------------------------------------------

isotig69710                         CATGC
ENSG00000108691:ENST0000058090      -----
ENSG00000108691:ENST0000022583      -----


""",
        )


class Test_build(unittest.TestCase):
    def test_build1(self):
        aligner = CodonAligner()
        codon_alignments = []
        seq1 = SeqRecord(
            Seq(
                "TCAGGGACTGCGAGAACCAAGCTACTGCTGCTGCTGGCTGCGCTCTGCGCCGCAGGTGGGGCGCTGGAG"
            ),
            id="pro1",
        )
        seq2 = SeqRecord(
            Seq("TCAGGGACTTCGAGAACCAAGCGCTCCTGCTGCTGGCTGCGCTCGGCGCCGCAGGTGGAGCACTGGAG"),
            id="pro2",
        )
        pro1 = SeqRecord(Seq("SGTARTKLLLLLAALCAAGGALE"), id="pro1")
        pro2 = SeqRecord(Seq("SGTSRTKRLLLLAALGAAGGALE"), id="pro2")
        alignments = aligner.align(pro1, seq1)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 23], [0, 69]]))
        )
        self.assertEqual(
            str(alignment),
            """\
pro1              0 S  G  T  A  R  T  K  L  L  L  L  L  A  A  L  C  A  A  G  G  
pro1              0 TCAGGGACTGCGAGAACCAAGCTACTGCTGCTGCTGGCTGCGCTCTGCGCCGCAGGTGGG

pro1             20 A  L  E   23
pro1             60 GCGCTGGAG 69
""",
        )
        alignments = aligner.align(pro2, seq2)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates, np.array([[0, 8, 8, 23], [0, 24, 23, 68]])
            )
        )
        self.assertEqual(
            str(alignment),
            """\
pro2              0 S  G  T  S  R  T  K  R   8
pro2              0 TCAGGGACTTCGAGAACCAAGCGC 24

pro2              8 L  L  L  L  A  A  L  G  A  A  G  G  A  L  E   23
pro2             23 CTCCTGCTGCTGGCTGCGCTCGGCGCCGCAGGTGGAGCACTGGAG 68
""",
        )
        alignment = Alignment([pro1, pro2])
        alignment = alignment.mapall(codon_alignments)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
                np.array([[0, 24, 24, 69],
                          [0, 24, 23, 68]])
                # fmt: on
            )
        )
        self.assertEqual(
            str(alignment),
            """\
pro1              0 TCAGGGACTGCGAGAACCAAGCTA 24
                  0 |||||||||.||||||||||||..
pro2              0 TCAGGGACTTCGAGAACCAAGCGC 24

pro1             24 CTGCTGCTGCTGGCTGCGCTCTGCGCCGCAGGTGGGGCGCTGGAG 69
                 24 ||.||||||||||||||||||.|||||||||||||.||.|||||| 69
pro2             23 CTCCTGCTGCTGGCTGCGCTCGGCGCCGCAGGTGGAGCACTGGAG 68
""",
        )

    def test_build2(self):
        aligner = CodonAligner()
        codon_alignments = []
        seq1 = SeqRecord(
            Seq(
                "ATGAAAAAGCACGAGTTACTTTGCCAAGGGACAAGTAACAAGCTCACCCAGTTGGGCACTTTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAACTGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCTTTTCTCAAGACCATCCAGGAGGTTGCCGGCTATGTACTCATTGCCCTC"
            ),
            id="pro1",
        )
        seq2 = SeqRecord(
            Seq(
                "ATGAAAAAGCACGAGTTCTTTGCCAAGGGACAAGTAACAAGCTCACCCAGTTGGGCACTTTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAATGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCTTTTCTCAAGACCATCCAGGAGGTTGCCGGCTATGTACTCATTGCCCTC"
            ),
            id="pro2",
        )
        seq3 = SeqRecord(
            Seq(
                "ATGAAAAAGCACGAGTTACTTTGCCAAGGGACAAGTAACAAGCTCACCCTTGGGCACTTTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAACTGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCTTTTCTCAAGACCATCCAGGAGGTTGCCGGCTATGTACTCATTGCCCTC"
            ),
            id="pro3",
        )
        pro1 = SeqRecord(
            Seq(
                "MKKHELLCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYMQSSYNLSFLKTIQEVAGYVLIAL"
            ),
            id="pro1",
        )
        pro2 = SeqRecord(
            Seq(
                "MKKHEFLCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYMQSSYNLSFLKTIQEVAGYVLIAL"
            ),
            id="pro2",
        )
        pro3 = SeqRecord(
            Seq(
                "MKKHELLCQGTSNKLTLLGTFEDHFLSLQRMFNNCEVVLGNLEITYMQSSYNLSFLKTIQEVAGYVLIAL"
            ),
            id="pro3",
        )
        alignments = aligner.align(pro1, seq1)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 70], [0, 210]]))
        )
        self.assertEqual(
            str(alignment),
            """\
pro1              0 M  K  K  H  E  L  L  C  Q  G  T  S  N  K  L  T  Q  L  G  T  
pro1              0 ATGAAAAAGCACGAGTTACTTTGCCAAGGGACAAGTAACAAGCTCACCCAGTTGGGCACT

pro1             20 F  E  D  H  F  L  S  L  Q  R  M  F  N  N  C  E  V  V  L  G  
pro1             60 TTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAACTGTGAGGTGGTCCTTGGG

pro1             40 N  L  E  I  T  Y  M  Q  S  S  Y  N  L  S  F  L  K  T  I  Q  
pro1            120 AATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCTTTTCTCAAGACCATCCAG

pro1             60 E  V  A  G  Y  V  L  I  A  L    70
pro1            180 GAGGTTGCCGGCTATGTACTCATTGCCCTC 210
""",
        )
        alignments = aligner.align(pro2, seq2)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                np.array([[0, 6, 6, 34, 34, 70], [0, 18, 17, 101, 100, 208]]),
            )
        )
        self.assertEqual(
            str(alignment),
            """\
pro2              0 M  K  K  H  E  F   6
pro2              0 ATGAAAAAGCACGAGTTC 18

pro2              6 L  C  Q  G  T  S  N  K  L  T  Q  L  G  T  F  E  D  H  F  L  
pro2             17 CTTTGCCAAGGGACAAGTAACAAGCTCACCCAGTTGGGCACTTTTGAAGACCACTTTCTG

pro2             26 S  L  Q  R  M  F  N  N   34
pro2             77 AGCCTACAGAGGATGTTCAACAAT 101

pro2             34 C  E  V  V  L  G  N  L  E  I  T  Y  M  Q  S  S  Y  N  L  S  
pro2            100 TGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCT

pro2             54 F  L  K  T  I  Q  E  V  A  G  Y  V  L  I  A  L    70
pro2            160 TTTCTCAAGACCATCCAGGAGGTTGCCGGCTATGTACTCATTGCCCTC 208
""",
        )
        alignments = aligner.align(pro3, seq3)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates, np.array([[0, 17, 17, 70], [0, 51, 49, 208]])
            )
        )
        self.assertEqual(
            str(alignment),
            """\
pro3              0 M  K  K  H  E  L  L  C  Q  G  T  S  N  K  L  T  L   17
pro3              0 ATGAAAAAGCACGAGTTACTTTGCCAAGGGACAAGTAACAAGCTCACCCTT 51

pro3             17 L  G  T  F  E  D  H  F  L  S  L  Q  R  M  F  N  N  C  E  V  
pro3             49 TTGGGCACTTTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAACTGTGAGGTG

pro3             37 V  L  G  N  L  E  I  T  Y  M  Q  S  S  Y  N  L  S  F  L  K  
pro3            109 GTCCTTGGGAATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCTTTTCTCAAG

pro3             57 T  I  Q  E  V  A  G  Y  V  L  I  A  L    70
pro3            169 ACCATCCAGGAGGTTGCCGGCTATGTACTCATTGCCCTC 208
""",
        )
        alignment = Alignment([pro1, pro2, pro3])
        alignment = alignment.mapall(codon_alignments)
        self.assertEqual(
            str(alignment),
            """\
pro1              0 ATGAAAAAGCACGAGTTA 18
pro2              0 ATGAAAAAGCACGAGTTC 18
pro3              0 ATGAAAAAGCACGAGTTA 18

pro1             18 CTTTGCCAAGGGACAAGTAACAAGCTCACCCAG 51
pro2             17 CTTTGCCAAGGGACAAGTAACAAGCTCACCCAG 50
pro3             18 CTTTGCCAAGGGACAAGTAACAAGCTCACCCTT 51

pro1             51 TTGGGCACTTTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAAC 102
pro2             50 TTGGGCACTTTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAAT 101
pro3             49 TTGGGCACTTTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAAC 100

pro1            102 TGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCT
pro2            100 TGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCT
pro3            100 TGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCT

pro1            162 TTTCTCAAGACCATCCAGGAGGTTGCCGGCTATGTACTCATTGCCCTC 210
pro2            160 TTTCTCAAGACCATCCAGGAGGTTGCCGGCTATGTACTCATTGCCCTC 208
pro3            160 TTTCTCAAGACCATCCAGGAGGTTGCCGGCTATGTACTCATTGCCCTC 208
""",
        )
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
                np.array([[0, 18, 18, 51, 51, 102, 102, 210],
                          [0, 18, 17, 50, 50, 101, 100, 208],
                          [0, 18, 18, 51, 49, 100, 100, 208]])
                # fmt: on
            )
        )

    def test_build3(self):
        # use Yeast mitochondrial codon table
        codon_table = CodonTable.unambiguous_dna_by_id[3]
        aligner = CodonAligner(codon_table=codon_table)
        codon_alignments = []
        seq1 = SeqRecord(
            Seq(
                "ATGGCAAGGGACCACCCAGTTGGGCACTGATATGATCGGGTGTATTTGCAGAGTAGTAACCTTTCTTTTCTCAAGACCATCCAG"
            ),
            id="pro1",
        )
        seq2 = SeqRecord(
            Seq(
                "ATGGCAAGGCACCATCCAGTTGAGCACTGATATGATCGGGTGTATTTGCAGAGTAGTAACGTGTCTCTGCTCAAGACCATCCAG"
            ),
            id="pro2",
        )
        seq3 = SeqRecord(
            Seq(
                "ATGGCAGGGGACCACCCAGTTGGGCACTGATATGATCGTGTGTATCTGCAGAGTAGTAACCACTCTTTTCTCATGACCATCCAG"
            ),
            id="pro3",
        )
        pro1 = SeqRecord(Seq("MARDHPVGHWYDRVYLQSSNTSFTKTIQ"), id="pro1")
        pro2 = SeqRecord(Seq("MARHHPVEHWYDRVYLQSSNVSTTKTIQ"), id="pro2")
        pro3 = SeqRecord(Seq("MAGDHPVGHWYDRVYTQSSNHSFTMTIQ"), id="pro3")
        alignments = aligner.align(pro1, seq1)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 28], [0, 84]]))
        )
        self.assertEqual(
            str(alignment),
            """\
pro1              0 M  A  R  D  H  P  V  G  H  W  Y  D  R  V  Y  L  Q  S  S  N  
pro1              0 ATGGCAAGGGACCACCCAGTTGGGCACTGATATGATCGGGTGTATTTGCAGAGTAGTAAC

pro1             20 T  S  F  T  K  T  I  Q   28
pro1             60 CTTTCTTTTCTCAAGACCATCCAG 84
""",
        )
        alignments = aligner.align(pro2, seq2)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 28], [0, 84]]))
        )
        self.assertEqual(
            str(alignment),
            """\
pro2              0 M  A  R  H  H  P  V  E  H  W  Y  D  R  V  Y  L  Q  S  S  N  
pro2              0 ATGGCAAGGCACCATCCAGTTGAGCACTGATATGATCGGGTGTATTTGCAGAGTAGTAAC

pro2             20 V  S  T  T  K  T  I  Q   28
pro2             60 GTGTCTCTGCTCAAGACCATCCAG 84
""",
        )
        alignments = aligner.align(pro3, seq3)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 28], [0, 84]]))
        )
        self.assertEqual(
            str(alignment),
            """\
pro3              0 M  A  G  D  H  P  V  G  H  W  Y  D  R  V  Y  T  Q  S  S  N  
pro3              0 ATGGCAGGGGACCACCCAGTTGGGCACTGATATGATCGTGTGTATCTGCAGAGTAGTAAC

pro3             20 H  S  F  T  M  T  I  Q   28
pro3             60 CACTCTTTTCTCATGACCATCCAG 84
""",
        )
        alignment = Alignment([pro1, pro2, pro3])
        alignment = alignment.mapall(codon_alignments)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
                np.array([[0, 84],
                          [0, 84],
                          [0, 84]])
                # fmt: on
            )
        )
        self.assertEqual(
            str(alignment),
            """\
pro1              0 ATGGCAAGGGACCACCCAGTTGGGCACTGATATGATCGGGTGTATTTGCAGAGTAGTAAC
pro2              0 ATGGCAAGGCACCATCCAGTTGAGCACTGATATGATCGGGTGTATTTGCAGAGTAGTAAC
pro3              0 ATGGCAGGGGACCACCCAGTTGGGCACTGATATGATCGTGTGTATCTGCAGAGTAGTAAC

pro1             60 CTTTCTTTTCTCAAGACCATCCAG 84
pro2             60 GTGTCTCTGCTCAAGACCATCCAG 84
pro3             60 CACTCTTTTCTCATGACCATCCAG 84
""",
        )


class Test_dn_ds(unittest.TestCase):
    def test_dn_ds(self):
        aligner = CodonAligner()
        nucleotide_records = SeqIO.index("codonalign/egfr_nucl.fa", "fasta")
        protein_alignment = Align.read("codonalign/egfr_pro.aln", "clustal")
        self.assertEqual(len(protein_alignment.sequences), 6)
        codon_alignments = []
        protein_record = protein_alignment.sequences[0]
        self.assertEqual(protein_record.id, "gi|17136534|ref|NP_476758.1|")
        nucleotide_record = nucleotide_records["gi|24657088|ref|NM_057410.3|"]
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1377], [84, 4215]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|171365         0 M  M  I  I  S  M  W  M  S  I  S  R  G  L  W  D  S  S  S  I  
gi|246570        84 ATGATGATTATCAGCATGTGGATGAGCATATCGCGAGGATTGTGGGACAGCAGCTCCATC

gi|171365        20 W  S  V  L  L  I  L  A  C  M  A  S  I  T  T  S  S  S  V  S  
gi|246570       144 TGGTCGGTGCTGCTGATCCTCGCCTGCATGGCATCCATCACCACAAGCTCATCGGTCAGC

gi|171365        40 N  A  G  Y  V  D  N  G  N  M  K  V  C  I  G  T  K  S  R  L  
gi|246570       204 AATGCCGGCTATGTGGATAATGGCAATATGAAAGTCTGCATCGGCACTAAATCTCGGCTC

gi|171365        60 S  V  P  S  N  K  E  H  H  Y  R  N  L  R  D  R  Y  T  N  C  
gi|246570       264 TCCGTGCCCTCCAACAAGGAACATCATTACCGGAACCTCAGAGATCGGTACACGAACTGT

gi|171365        80 T  Y  V  D  G  N  L  E  L  T  W  L  P  N  E  N  L  D  L  S  
gi|246570       324 ACGTATGTGGATGGCAACCTGGAGCTGACCTGGCTGCCCAACGAGAATTTGGACCTCAGC

gi|171365       100 F  L  D  N  I  R  E  V  T  G  Y  I  L  I  S  H  V  D  V  K  
gi|246570       384 TTCCTGGACAACATACGGGAGGTCACCGGCTATATTCTGATCAGTCATGTGGACGTTAAG

gi|171365       120 K  V  V  F  P  K  L  Q  I  I  R  G  R  T  L  F  S  L  S  V  
gi|246570       444 AAAGTGGTATTTCCCAAACTACAAATCATTCGCGGACGCACGCTGTTCAGCTTATCCGTG

gi|171365       140 E  E  E  K  Y  A  L  F  V  T  Y  S  K  M  Y  T  L  E  I  P  
gi|246570       504 GAGGAGGAGAAGTATGCCTTGTTCGTCACTTATTCCAAAATGTACACGCTGGAGATTCCC

gi|171365       160 D  L  R  D  V  L  N  G  Q  V  G  F  H  N  N  Y  N  L  C  H  
gi|246570       564 GATCTACGCGATGTCTTAAATGGCCAAGTGGGCTTCCACAACAACTACAATCTCTGCCAC

gi|171365       180 M  R  T  I  Q  W  S  E  I  V  S  N  G  T  D  A  Y  Y  N  Y  
gi|246570       624 ATGCGAACGATCCAGTGGTCGGAGATTGTATCCAACGGCACGGATGCATACTACAACTAC

gi|171365       200 D  F  T  A  P  E  R  E  C  P  K  C  H  E  S  C  T  H  G  C  
gi|246570       684 GACTTTACTGCTCCGGAGCGCGAGTGTCCCAAGTGCCACGAGAGCTGCACGCACGGATGT

gi|171365       220 W  G  E  G  P  K  N  C  Q  K  F  S  K  L  T  C  S  P  Q  C  
gi|246570       744 TGGGGCGAGGGTCCCAAGAATTGCCAGAAGTTCAGCAAGCTCACCTGCTCGCCACAGTGT

gi|171365       240 A  G  G  R  C  Y  G  P  K  P  R  E  C  C  H  L  F  C  A  G  
gi|246570       804 GCCGGAGGTCGTTGCTATGGACCAAAGCCGCGGGAGTGTTGTCACCTCTTCTGCGCCGGA

gi|171365       260 G  C  T  G  P  T  Q  K  D  C  I  A  C  K  N  F  F  D  E  G  
gi|246570       864 GGATGCACTGGTCCCACGCAAAAGGATTGCATCGCCTGCAAGAACTTCTTCGACGAGGGC

gi|171365       280 V  C  K  E  E  C  P  P  M  R  K  Y  N  P  T  T  Y  V  L  E  
gi|246570       924 GTATGCAAGGAGGAATGCCCGCCCATGCGCAAGTACAATCCCACCACCTATGTTCTTGAA

gi|171365       300 T  N  P  E  G  K  Y  A  Y  G  A  T  C  V  K  E  C  P  G  H  
gi|246570       984 ACGAATCCTGAGGGAAAGTATGCCTATGGTGCCACCTGCGTCAAGGAGTGTCCCGGTCAT

gi|171365       320 L  L  R  D  N  G  A  C  V  R  S  C  P  Q  D  K  M  D  K  G  
gi|246570      1044 CTGTTGCGTGATAATGGCGCCTGCGTGCGCAGCTGTCCCCAGGACAAGATGGACAAGGGG

gi|171365       340 G  E  C  V  P  C  N  G  P  C  P  K  T  C  P  G  V  T  V  L  
gi|246570      1104 GGCGAGTGTGTGCCCTGCAATGGACCGTGCCCCAAAACCTGCCCGGGCGTTACTGTCCTG

gi|171365       360 H  A  G  N  I  D  S  F  R  N  C  T  V  I  D  G  N  I  R  I  
gi|246570      1164 CATGCCGGCAACATTGACTCGTTCCGGAATTGTACGGTGATCGATGGCAACATTCGCATT

gi|171365       380 L  D  Q  T  F  S  G  F  Q  D  V  Y  A  N  Y  T  M  G  P  R  
gi|246570      1224 TTGGATCAGACCTTCTCGGGCTTCCAGGATGTCTATGCCAACTACACGATGGGACCACGA

gi|171365       400 Y  I  P  L  D  P  E  R  L  E  V  F  S  T  V  K  E  I  T  G  
gi|246570      1284 TACATACCGCTGGATCCCGAGCGACTGGAGGTGTTCTCCACGGTGAAGGAGATCACCGGG

gi|171365       420 Y  L  N  I  E  G  T  H  P  Q  F  R  N  L  S  Y  F  R  N  L  
gi|246570      1344 TATCTGAATATCGAGGGAACCCACCCGCAGTTCCGGAATCTGTCGTACTTCCGCAATCTG

gi|171365       440 E  T  I  H  G  R  Q  L  M  E  S  M  F  A  A  L  A  I  V  K  
gi|246570      1404 GAAACAATTCATGGCCGCCAGCTGATGGAGAGCATGTTTGCCGCTTTGGCGATCGTTAAG

gi|171365       460 S  S  L  Y  S  L  E  M  R  N  L  K  Q  I  S  S  G  S  V  V  
gi|246570      1464 TCATCCCTGTACAGCCTGGAGATGCGCAATCTGAAGCAGATTAGTTCCGGCAGTGTGGTC

gi|171365       480 I  Q  H  N  R  D  L  C  Y  V  S  N  I  R  W  P  A  I  Q  K  
gi|246570      1524 ATCCAGCATAATAGAGACCTCTGCTACGTAAGCAATATCCGTTGGCCGGCCATTCAGAAG

gi|171365       500 E  P  E  Q  K  V  W  V  N  E  N  L  R  A  D  L  C  E  K  N  
gi|246570      1584 GAGCCCGAACAGAAGGTGTGGGTCAACGAGAATCTCAGGGCGGATCTATGCGAGAAAAAT

gi|171365       520 G  T  I  C  S  D  Q  C  N  E  D  G  C  W  G  A  G  T  D  Q  
gi|246570      1644 GGAACCATTTGCTCGGATCAGTGCAACGAGGACGGCTGCTGGGGAGCTGGCACGGATCAG

gi|171365       540 C  L  T  C  K  N  F  N  F  N  G  T  C  I  A  D  C  G  Y  I  
gi|246570      1704 TGCCTTACCTGCAAGAACTTCAATTTCAATGGCACCTGCATCGCCGACTGTGGTTATATA

gi|171365       560 S  N  A  Y  K  F  D  N  R  T  C  K  I  C  H  P  E  C  R  T  
gi|246570      1764 TCCAATGCCTACAAGTTTGACAATAGAACGTGCAAGATATGCCATCCAGAGTGCCGGACT

gi|171365       580 C  N  G  A  G  A  D  H  C  Q  E  C  V  H  V  R  D  G  Q  H  
gi|246570      1824 TGCAATGGAGCTGGAGCAGATCACTGCCAGGAGTGCGTCCATGTGAGGGACGGTCAGCAC

gi|171365       600 C  V  S  E  C  P  K  N  K  Y  N  D  R  G  V  C  R  E  C  H  
gi|246570      1884 TGTGTGTCCGAGTGCCCGAAGAACAAGTACAACGATCGTGGTGTCTGCCGAGAGTGCCAC

gi|171365       620 A  T  C  D  G  C  T  G  P  K  D  T  I  G  I  G  A  C  T  T  
gi|246570      1944 GCCACCTGCGATGGATGCACTGGGCCCAAGGACACCATCGGCATTGGAGCGTGTACAACG

gi|171365       640 C  N  L  A  I  I  N  N  D  A  T  V  K  R  C  L  L  K  D  D  
gi|246570      2004 TGCAATTTGGCCATTATCAACAATGACGCCACAGTAAAACGCTGCCTGCTGAAGGACGAC

gi|171365       660 K  C  P  D  G  Y  F  W  E  Y  V  H  P  Q  E  Q  G  S  L  K  
gi|246570      2064 AAGTGCCCCGATGGGTACTTCTGGGAGTATGTGCATCCACAAGAGCAGGGATCGCTAAAG

gi|171365       680 P  L  A  G  R  A  V  C  R  K  C  H  P  L  C  E  L  C  T  N  
gi|246570      2124 CCATTGGCCGGCAGAGCAGTTTGCCGAAAGTGCCATCCCCTTTGCGAGCTGTGCACCAAC

gi|171365       700 Y  G  Y  H  E  Q  V  C  S  K  C  T  H  Y  K  R  R  E  Q  C  
gi|246570      2184 TACGGATACCATGAACAGGTGTGCTCCAAGTGCACCCACTACAAGCGACGAGAGCAGTGC

gi|171365       720 E  T  E  C  P  A  D  H  Y  T  D  E  E  Q  R  E  C  F  Q  C  
gi|246570      2244 GAGACCGAGTGTCCGGCCGATCACTACACGGATGAGGAGCAGCGCGAGTGCTTCCAGTGC

gi|171365       740 H  P  E  C  N  G  C  T  G  P  G  A  D  D  C  K  S  C  R  N  
gi|246570      2304 CACCCAGAATGCAACGGTTGCACTGGTCCGGGTGCCGACGATTGCAAGTCTTGTCGCAAC

gi|171365       760 F  K  L  F  D  A  N  E  T  G  P  Y  V  N  S  T  M  F  N  C  
gi|246570      2364 TTCAAGTTGTTCGACGCGAATGAGACGGGTCCCTATGTGAACTCCACGATGTTCAATTGC

gi|171365       780 T  S  K  C  P  L  E  M  R  H  V  N  Y  Q  Y  T  A  I  G  P  
gi|246570      2424 ACCTCGAAGTGTCCCTTGGAGATGCGACATGTGAACTATCAGTACACGGCCATTGGACCC

gi|171365       800 Y  C  A  A  S  P  P  R  S  S  K  I  T  A  N  L  D  V  N  M  
gi|246570      2484 TACTGTGCAGCTAGTCCGCCGAGGAGCAGCAAGATAACTGCCAATCTGGATGTGAACATG

gi|171365       820 I  F  I  I  T  G  A  V  L  V  P  T  I  C  I  L  C  V  V  T  
gi|246570      2544 ATCTTCATTATCACTGGTGCTGTTCTGGTGCCGACGATCTGCATCCTCTGCGTGGTCACA

gi|171365       840 Y  I  C  R  Q  K  Q  K  A  K  K  E  T  V  K  M  T  M  A  L  
gi|246570      2604 TACATTTGTCGGCAAAAGCAAAAGGCCAAGAAAGAAACAGTGAAGATGACCATGGCTCTG

gi|171365       860 S  G  C  E  D  S  E  P  L  R  P  S  N  I  G  A  N  L  C  K  
gi|246570      2664 TCCGGCTGTGAGGATTCCGAGCCGCTGCGTCCCTCGAACATTGGAGCCAATCTATGCAAG

gi|171365       880 L  R  I  V  K  D  A  E  L  R  K  G  G  V  L  G  M  G  A  F  
gi|246570      2724 TTGCGCATTGTCAAGGACGCCGAGTTGCGCAAGGGCGGAGTCCTCGGAATGGGAGCCTTT

gi|171365       900 G  R  V  Y  K  G  V  W  V  P  E  G  E  N  V  K  I  P  V  A  
gi|246570      2784 GGACGAGTGTACAAGGGCGTTTGGGTGCCGGAGGGTGAGAACGTCAAGATTCCAGTGGCC

gi|171365       920 I  K  E  L  L  K  S  T  G  A  E  S  S  E  E  F  L  R  E  A  
gi|246570      2844 ATTAAGGAGCTGCTCAAGTCCACAGGCGCCGAGTCAAGCGAAGAGTTCCTCCGCGAAGCC

gi|171365       940 Y  I  M  A  S  V  E  H  V  N  L  L  K  L  L  A  V  C  M  S  
gi|246570      2904 TACATCATGGCCTCTGTGGAGCACGTTAATCTGCTGAAGCTCCTGGCCGTCTGCATGTCC

gi|171365       960 S  Q  M  M  L  I  T  Q  L  M  P  L  G  C  L  L  D  Y  V  R  
gi|246570      2964 TCACAAATGATGCTAATCACGCAACTGATGCCGCTTGGCTGCCTGTTGGACTATGTGCGA

gi|171365       980 N  N  R  D  K  I  G  S  K  A  L  L  N  W  S  T  Q  I  A  K  
gi|246570      3024 AATAACCGGGACAAGATCGGCTCTAAGGCTCTGCTCAACTGGAGCACGCAAATCGCCAAG

gi|171365      1000 G  M  S  Y  L  E  E  K  R  L  V  H  R  D  L  A  A  R  N  V  
gi|246570      3084 GGCATGTCGTATCTGGAGGAGAAGCGACTGGTCCACAGAGACTTGGCTGCCCGCAATGTC

gi|171365      1020 L  V  Q  T  P  S  L  V  K  I  T  D  F  G  L  A  K  L  L  S  
gi|246570      3144 CTGGTGCAGACTCCCTCGCTGGTGAAGATCACCGACTTTGGGCTGGCCAAGTTGCTGAGC

gi|171365      1040 S  D  S  N  E  Y  K  A  A  G  G  K  M  P  I  K  W  L  A  L  
gi|246570      3204 AGCGATTCCAATGAGTACAAGGCTGCTGGCGGCAAGATGCCCATCAAGTGGTTGGCACTG

gi|171365      1060 E  C  I  R  N  R  V  F  T  S  K  S  D  V  W  A  F  G  V  T  
gi|246570      3264 GAGTGCATTCGCAATCGTGTATTCACCAGCAAGTCCGATGTCTGGGCCTTTGGTGTGACA

gi|171365      1080 I  W  E  L  L  T  F  G  Q  R  P  H  E  N  I  P  A  K  D  I  
gi|246570      3324 ATTTGGGAACTGCTGACCTTTGGCCAGCGTCCACACGAGAACATCCCCGCTAAGGATATT

gi|171365      1100 P  D  L  I  E  V  G  L  K  L  E  Q  P  E  I  C  S  L  D  I  
gi|246570      3384 CCCGATCTTATTGAAGTCGGTCTGAAGCTGGAGCAGCCGGAGATTTGTTCGCTGGACATT

gi|171365      1120 Y  C  T  L  L  S  C  W  H  L  D  A  A  M  R  P  T  F  K  Q  
gi|246570      3444 TACTGCACACTTCTCTCGTGCTGGCACTTGGATGCCGCCATGCGTCCAACCTTCAAGCAG

gi|171365      1140 L  T  T  V  F  A  E  F  A  R  D  P  G  R  Y  L  A  I  P  G  
gi|246570      3504 CTGACTACGGTCTTTGCTGAGTTCGCCAGAGATCCGGGTCGCTATCTGGCCATTCCCGGG

gi|171365      1160 D  K  F  T  R  L  P  A  Y  T  S  Q  D  E  K  D  L  I  R  K  
gi|246570      3564 GATAAGTTCACCCGGCTGCCGGCCTACACGAGTCAGGATGAGAAGGATCTCATCCGAAAA

gi|171365      1180 L  A  P  T  T  D  G  S  E  A  I  A  E  P  D  D  Y  L  Q  P  
gi|246570      3624 TTGGCTCCCACCACCGATGGGTCCGAAGCCATTGCGGAACCCGATGACTACCTGCAACCC

gi|171365      1200 K  A  A  P  G  P  S  H  R  T  D  C  T  D  E  I  P  K  L  N  
gi|246570      3684 AAGGCAGCACCTGGTCCTAGTCACAGAACCGACTGCACGGATGAGATACCCAAGCTGAAC

gi|171365      1220 R  Y  C  K  D  P  S  N  K  N  S  S  T  G  D  D  E  T  D  S  
gi|246570      3744 CGCTACTGCAAGGATCCTAGCAACAAGAATTCGAGTACCGGAGACGATGAGACGGATTCG

gi|171365      1240 S  A  R  E  V  G  V  G  N  L  R  L  D  L  P  V  D  E  D  D  
gi|246570      3804 AGTGCCCGGGAAGTGGGCGTGGGTAATCTGCGCCTCGATCTACCAGTCGATGAGGATGAT

gi|171365      1260 Y  L  M  P  T  C  Q  P  G  P  N  N  N  N  N  I  N  N  P  N  
gi|246570      3864 TACCTGATGCCCACATGCCAACCGGGGCCCAACAACAACAACAACATAAATAATCCCAAT

gi|171365      1280 Q  N  N  M  A  A  V  G  V  A  A  G  Y  M  D  L  I  G  V  P  
gi|246570      3924 CAAAACAATATGGCAGCTGTGGGCGTGGCTGCCGGCTACATGGATCTCATCGGAGTGCCC

gi|171365      1300 V  S  V  D  N  P  E  Y  L  L  N  A  Q  T  L  G  V  G  E  S  
gi|246570      3984 GTTAGTGTGGACAATCCGGAGTATCTGCTAAACGCGCAGACACTGGGTGTTGGGGAGTCG

gi|171365      1320 P  I  P  T  Q  T  I  G  I  P  V  M  G  V  P  G  T  M  E  V  
gi|246570      4044 CCGATACCCACCCAGACCATCGGGATACCGGTGATGGGAGTCCCGGGCACCATGGAGGTC

gi|171365      1340 K  V  P  M  P  G  S  E  P  T  S  S  D  H  E  Y  Y  N  D  T  
gi|246570      4104 AAGGTGCCAATGCCAGGCAGTGAGCCAACGAGCTCCGATCACGAGTACTACAATGATACC

gi|171365      1360 Q  R  E  L  Q  P  L  H  R  N  R  N  T  E  T  R  V   1377
gi|246570      4164 CAACGGGAGTTGCAGCCACTGCATCGAAACCGCAACACGGAGACGAGGGTG 4215
""",
        )
        protein_record = protein_alignment.sequences[1]
        self.assertEqual(protein_record.id, "gi|17136536|ref|NP_476759.1|")
        nucleotide_record = nucleotide_records["gi|24657104|ref|NM_057411.3|"]
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1426], [22, 4300]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|171365         0 M  L  L  R  R  R  N  G  P  C  P  F  P  L  L  L  L  L  L  A  
gi|246571        22 ATGCTGCTGCGACGGCGCAACGGCCCCTGCCCCTTCCCCCTGCTGCTCCTGCTCCTGGCC

gi|171365        20 H  C  I  C  I  W  P  A  S  A  A  R  D  R  Y  A  R  Q  N  N  
gi|246571        82 CACTGCATTTGCATTTGGCCCGCGTCGGCGGCCCGCGATCGCTACGCCCGCCAGAACAAT

gi|171365        40 R  Q  R  H  Q  D  I  D  R  D  R  D  R  D  R  F  L  Y  R  S  
gi|246571       142 CGCCAGCGCCATCAGGATATAGATCGCGATCGGGATCGAGATCGATTCCTATACCGCAGC

gi|171365        60 S  S  A  Q  N  R  Q  R  G  G  A  N  F  A  L  G  L  G  A  N  
gi|246571       202 AGTTCGGCCCAAAATCGACAGAGGGGCGGGGCCAACTTCGCCCTGGGACTGGGAGCCAAC

gi|171365        80 G  V  T  I  P  T  S  L  E  D  K  N  K  N  E  F  V  K  G  K  
gi|246571       262 GGAGTCACCATTCCCACCAGTCTGGAGGATAAGAACAAGAACGAGTTCGTCAAGGGGAAA

gi|171365       100 I  C  I  G  T  K  S  R  L  S  V  P  S  N  K  E  H  H  Y  R  
gi|246571       322 ATCTGCATCGGCACTAAATCTCGGCTCTCCGTGCCCTCCAACAAGGAACATCATTACCGG

gi|171365       120 N  L  R  D  R  Y  T  N  C  T  Y  V  D  G  N  L  E  L  T  W  
gi|246571       382 AACCTCAGAGATCGGTACACGAACTGTACGTATGTGGATGGCAACCTGGAGCTGACCTGG

gi|171365       140 L  P  N  E  N  L  D  L  S  F  L  D  N  I  R  E  V  T  G  Y  
gi|246571       442 CTGCCCAACGAGAATTTGGACCTCAGCTTCCTGGACAACATACGGGAGGTCACCGGCTAT

gi|171365       160 I  L  I  S  H  V  D  V  K  K  V  V  F  P  K  L  Q  I  I  R  
gi|246571       502 ATTCTGATCAGTCATGTGGACGTTAAGAAAGTGGTATTTCCCAAACTACAAATCATTCGC

gi|171365       180 G  R  T  L  F  S  L  S  V  E  E  E  K  Y  A  L  F  V  T  Y  
gi|246571       562 GGACGCACGCTGTTCAGCTTATCCGTGGAGGAGGAGAAGTATGCCTTGTTCGTCACTTAT

gi|171365       200 S  K  M  Y  T  L  E  I  P  D  L  R  D  V  L  N  G  Q  V  G  
gi|246571       622 TCCAAAATGTACACGCTGGAGATTCCCGATCTACGCGATGTCTTAAATGGCCAAGTGGGC

gi|171365       220 F  H  N  N  Y  N  L  C  H  M  R  T  I  Q  W  S  E  I  V  S  
gi|246571       682 TTCCACAACAACTACAATCTCTGCCACATGCGAACGATCCAGTGGTCGGAGATTGTATCC

gi|171365       240 N  G  T  D  A  Y  Y  N  Y  D  F  T  A  P  E  R  E  C  P  K  
gi|246571       742 AACGGCACGGATGCATACTACAACTACGACTTTACTGCTCCGGAGCGCGAGTGTCCCAAG

gi|171365       260 C  H  E  S  C  T  H  G  C  W  G  E  G  P  K  N  C  Q  K  F  
gi|246571       802 TGCCACGAGAGCTGCACGCACGGATGTTGGGGCGAGGGTCCCAAGAATTGCCAGAAGTTC

gi|171365       280 S  K  L  T  C  S  P  Q  C  A  G  G  R  C  Y  G  P  K  P  R  
gi|246571       862 AGCAAGCTCACCTGCTCGCCACAGTGTGCCGGAGGTCGTTGCTATGGACCAAAGCCGCGG

gi|171365       300 E  C  C  H  L  F  C  A  G  G  C  T  G  P  T  Q  K  D  C  I  
gi|246571       922 GAGTGTTGTCACCTCTTCTGCGCCGGAGGATGCACTGGTCCCACGCAAAAGGATTGCATC

gi|171365       320 A  C  K  N  F  F  D  E  G  V  C  K  E  E  C  P  P  M  R  K  
gi|246571       982 GCCTGCAAGAACTTCTTCGACGAGGGCGTATGCAAGGAGGAATGCCCGCCCATGCGCAAG

gi|171365       340 Y  N  P  T  T  Y  V  L  E  T  N  P  E  G  K  Y  A  Y  G  A  
gi|246571      1042 TACAATCCCACCACCTATGTTCTTGAAACGAATCCTGAGGGAAAGTATGCCTATGGTGCC

gi|171365       360 T  C  V  K  E  C  P  G  H  L  L  R  D  N  G  A  C  V  R  S  
gi|246571      1102 ACCTGCGTCAAGGAGTGTCCCGGTCATCTGTTGCGTGATAATGGCGCCTGCGTGCGCAGC

gi|171365       380 C  P  Q  D  K  M  D  K  G  G  E  C  V  P  C  N  G  P  C  P  
gi|246571      1162 TGTCCCCAGGACAAGATGGACAAGGGGGGCGAGTGTGTGCCCTGCAATGGACCGTGCCCC

gi|171365       400 K  T  C  P  G  V  T  V  L  H  A  G  N  I  D  S  F  R  N  C  
gi|246571      1222 AAAACCTGCCCGGGCGTTACTGTCCTGCATGCCGGCAACATTGACTCGTTCCGGAATTGT

gi|171365       420 T  V  I  D  G  N  I  R  I  L  D  Q  T  F  S  G  F  Q  D  V  
gi|246571      1282 ACGGTGATCGATGGCAACATTCGCATTTTGGATCAGACCTTCTCGGGCTTCCAGGATGTC

gi|171365       440 Y  A  N  Y  T  M  G  P  R  Y  I  P  L  D  P  E  R  L  E  V  
gi|246571      1342 TATGCCAACTACACGATGGGACCACGATACATACCGCTGGATCCCGAGCGACTGGAGGTG

gi|171365       460 F  S  T  V  K  E  I  T  G  Y  L  N  I  E  G  T  H  P  Q  F  
gi|246571      1402 TTCTCCACGGTGAAGGAGATCACCGGGTATCTGAATATCGAGGGAACCCACCCGCAGTTC

gi|171365       480 R  N  L  S  Y  F  R  N  L  E  T  I  H  G  R  Q  L  M  E  S  
gi|246571      1462 CGGAATCTGTCGTACTTCCGCAATCTGGAAACAATTCATGGCCGCCAGCTGATGGAGAGC

gi|171365       500 M  F  A  A  L  A  I  V  K  S  S  L  Y  S  L  E  M  R  N  L  
gi|246571      1522 ATGTTTGCCGCTTTGGCGATCGTTAAGTCATCCCTGTACAGCCTGGAGATGCGCAATCTG

gi|171365       520 K  Q  I  S  S  G  S  V  V  I  Q  H  N  R  D  L  C  Y  V  S  
gi|246571      1582 AAGCAGATTAGTTCCGGCAGTGTGGTCATCCAGCATAATAGAGACCTCTGCTACGTAAGC

gi|171365       540 N  I  R  W  P  A  I  Q  K  E  P  E  Q  K  V  W  V  N  E  N  
gi|246571      1642 AATATCCGTTGGCCGGCCATTCAGAAGGAGCCCGAACAGAAGGTGTGGGTCAACGAGAAT

gi|171365       560 L  R  A  D  L  C  E  K  N  G  T  I  C  S  D  Q  C  N  E  D  
gi|246571      1702 CTCAGGGCGGATCTATGCGAGAAAAATGGAACCATTTGCTCGGATCAGTGCAACGAGGAC

gi|171365       580 G  C  W  G  A  G  T  D  Q  C  L  T  C  K  N  F  N  F  N  G  
gi|246571      1762 GGCTGCTGGGGAGCTGGCACGGATCAGTGCCTTACCTGCAAGAACTTCAATTTCAATGGC

gi|171365       600 T  C  I  A  D  C  G  Y  I  S  N  A  Y  K  F  D  N  R  T  C  
gi|246571      1822 ACCTGCATCGCCGACTGTGGTTATATATCCAATGCCTACAAGTTTGACAATAGAACGTGC

gi|171365       620 K  I  C  H  P  E  C  R  T  C  N  G  A  G  A  D  H  C  Q  E  
gi|246571      1882 AAGATATGCCATCCAGAGTGCCGGACTTGCAATGGAGCTGGAGCAGATCACTGCCAGGAG

gi|171365       640 C  V  H  V  R  D  G  Q  H  C  V  S  E  C  P  K  N  K  Y  N  
gi|246571      1942 TGCGTCCATGTGAGGGACGGTCAGCACTGTGTGTCCGAGTGCCCGAAGAACAAGTACAAC

gi|171365       660 D  R  G  V  C  R  E  C  H  A  T  C  D  G  C  T  G  P  K  D  
gi|246571      2002 GATCGTGGTGTCTGCCGAGAGTGCCACGCCACCTGCGATGGATGCACTGGGCCCAAGGAC

gi|171365       680 T  I  G  I  G  A  C  T  T  C  N  L  A  I  I  N  N  D  A  T  
gi|246571      2062 ACCATCGGCATTGGAGCGTGTACAACGTGCAATTTGGCCATTATCAACAATGACGCCACA

gi|171365       700 V  K  R  C  L  L  K  D  D  K  C  P  D  G  Y  F  W  E  Y  V  
gi|246571      2122 GTAAAACGCTGCCTGCTGAAGGACGACAAGTGCCCCGATGGGTACTTCTGGGAGTATGTG

gi|171365       720 H  P  Q  E  Q  G  S  L  K  P  L  A  G  R  A  V  C  R  K  C  
gi|246571      2182 CATCCACAAGAGCAGGGATCGCTAAAGCCATTGGCCGGCAGAGCAGTTTGCCGAAAGTGC

gi|171365       740 H  P  L  C  E  L  C  T  N  Y  G  Y  H  E  Q  V  C  S  K  C  
gi|246571      2242 CATCCCCTTTGCGAGCTGTGCACCAACTACGGATACCATGAACAGGTGTGCTCCAAGTGC

gi|171365       760 T  H  Y  K  R  R  E  Q  C  E  T  E  C  P  A  D  H  Y  T  D  
gi|246571      2302 ACCCACTACAAGCGACGAGAGCAGTGCGAGACCGAGTGTCCGGCCGATCACTACACGGAT

gi|171365       780 E  E  Q  R  E  C  F  Q  C  H  P  E  C  N  G  C  T  G  P  G  
gi|246571      2362 GAGGAGCAGCGCGAGTGCTTCCAGTGCCACCCAGAATGCAACGGTTGCACTGGTCCGGGT

gi|171365       800 A  D  D  C  K  S  C  R  N  F  K  L  F  D  A  N  E  T  G  P  
gi|246571      2422 GCCGACGATTGCAAGTCTTGTCGCAACTTCAAGTTGTTCGACGCGAATGAGACGGGTCCC

gi|171365       820 Y  V  N  S  T  M  F  N  C  T  S  K  C  P  L  E  M  R  H  V  
gi|246571      2482 TATGTGAACTCCACGATGTTCAATTGCACCTCGAAGTGTCCCTTGGAGATGCGACATGTG

gi|171365       840 N  Y  Q  Y  T  A  I  G  P  Y  C  A  A  S  P  P  R  S  S  K  
gi|246571      2542 AACTATCAGTACACGGCCATTGGACCCTACTGTGCAGCTAGTCCGCCGAGGAGCAGCAAG

gi|171365       860 I  T  A  N  L  D  V  N  M  I  F  I  I  T  G  A  V  L  V  P  
gi|246571      2602 ATAACTGCCAATCTGGATGTGAACATGATCTTCATTATCACTGGTGCTGTTCTGGTGCCG

gi|171365       880 T  I  C  I  L  C  V  V  T  Y  I  C  R  Q  K  Q  K  A  K  K  
gi|246571      2662 ACGATCTGCATCCTCTGCGTGGTCACATACATTTGTCGGCAAAAGCAAAAGGCCAAGAAA

gi|171365       900 E  T  V  K  M  T  M  A  L  S  G  C  E  D  S  E  P  L  R  P  
gi|246571      2722 GAAACAGTGAAGATGACCATGGCTCTGTCCGGCTGTGAGGATTCCGAGCCGCTGCGTCCC

gi|171365       920 S  N  I  G  A  N  L  C  K  L  R  I  V  K  D  A  E  L  R  K  
gi|246571      2782 TCGAACATTGGAGCCAATCTATGCAAGTTGCGCATTGTCAAGGACGCCGAGTTGCGCAAG

gi|171365       940 G  G  V  L  G  M  G  A  F  G  R  V  Y  K  G  V  W  V  P  E  
gi|246571      2842 GGCGGAGTCCTCGGAATGGGAGCCTTTGGACGAGTGTACAAGGGCGTTTGGGTGCCGGAG

gi|171365       960 G  E  N  V  K  I  P  V  A  I  K  E  L  L  K  S  T  G  A  E  
gi|246571      2902 GGTGAGAACGTCAAGATTCCAGTGGCCATTAAGGAGCTGCTCAAGTCCACAGGCGCCGAG

gi|171365       980 S  S  E  E  F  L  R  E  A  Y  I  M  A  S  V  E  H  V  N  L  
gi|246571      2962 TCAAGCGAAGAGTTCCTCCGCGAAGCCTACATCATGGCCTCTGTGGAGCACGTTAATCTG

gi|171365      1000 L  K  L  L  A  V  C  M  S  S  Q  M  M  L  I  T  Q  L  M  P  
gi|246571      3022 CTGAAGCTCCTGGCCGTCTGCATGTCCTCACAAATGATGCTAATCACGCAACTGATGCCG

gi|171365      1020 L  G  C  L  L  D  Y  V  R  N  N  R  D  K  I  G  S  K  A  L  
gi|246571      3082 CTTGGCTGCCTGTTGGACTATGTGCGAAATAACCGGGACAAGATCGGCTCTAAGGCTCTG

gi|171365      1040 L  N  W  S  T  Q  I  A  K  G  M  S  Y  L  E  E  K  R  L  V  
gi|246571      3142 CTCAACTGGAGCACGCAAATCGCCAAGGGCATGTCGTATCTGGAGGAGAAGCGACTGGTC

gi|171365      1060 H  R  D  L  A  A  R  N  V  L  V  Q  T  P  S  L  V  K  I  T  
gi|246571      3202 CACAGAGACTTGGCTGCCCGCAATGTCCTGGTGCAGACTCCCTCGCTGGTGAAGATCACC

gi|171365      1080 D  F  G  L  A  K  L  L  S  S  D  S  N  E  Y  K  A  A  G  G  
gi|246571      3262 GACTTTGGGCTGGCCAAGTTGCTGAGCAGCGATTCCAATGAGTACAAGGCTGCTGGCGGC

gi|171365      1100 K  M  P  I  K  W  L  A  L  E  C  I  R  N  R  V  F  T  S  K  
gi|246571      3322 AAGATGCCCATCAAGTGGTTGGCACTGGAGTGCATTCGCAATCGTGTATTCACCAGCAAG

gi|171365      1120 S  D  V  W  A  F  G  V  T  I  W  E  L  L  T  F  G  Q  R  P  
gi|246571      3382 TCCGATGTCTGGGCCTTTGGTGTGACAATTTGGGAACTGCTGACCTTTGGCCAGCGTCCA

gi|171365      1140 H  E  N  I  P  A  K  D  I  P  D  L  I  E  V  G  L  K  L  E  
gi|246571      3442 CACGAGAACATCCCCGCTAAGGATATTCCCGATCTTATTGAAGTCGGTCTGAAGCTGGAG

gi|171365      1160 Q  P  E  I  C  S  L  D  I  Y  C  T  L  L  S  C  W  H  L  D  
gi|246571      3502 CAGCCGGAGATTTGTTCGCTGGACATTTACTGCACACTTCTCTCGTGCTGGCACTTGGAT

gi|171365      1180 A  A  M  R  P  T  F  K  Q  L  T  T  V  F  A  E  F  A  R  D  
gi|246571      3562 GCCGCCATGCGTCCAACCTTCAAGCAGCTGACTACGGTCTTTGCTGAGTTCGCCAGAGAT

gi|171365      1200 P  G  R  Y  L  A  I  P  G  D  K  F  T  R  L  P  A  Y  T  S  
gi|246571      3622 CCGGGTCGCTATCTGGCCATTCCCGGGGATAAGTTCACCCGGCTGCCGGCCTACACGAGT

gi|171365      1220 Q  D  E  K  D  L  I  R  K  L  A  P  T  T  D  G  S  E  A  I  
gi|246571      3682 CAGGATGAGAAGGATCTCATCCGAAAATTGGCTCCCACCACCGATGGGTCCGAAGCCATT

gi|171365      1240 A  E  P  D  D  Y  L  Q  P  K  A  A  P  G  P  S  H  R  T  D  
gi|246571      3742 GCGGAACCCGATGACTACCTGCAACCCAAGGCAGCACCTGGTCCTAGTCACAGAACCGAC

gi|171365      1260 C  T  D  E  I  P  K  L  N  R  Y  C  K  D  P  S  N  K  N  S  
gi|246571      3802 TGCACGGATGAGATACCCAAGCTGAACCGCTACTGCAAGGATCCTAGCAACAAGAATTCG

gi|171365      1280 S  T  G  D  D  E  T  D  S  S  A  R  E  V  G  V  G  N  L  R  
gi|246571      3862 AGTACCGGAGACGATGAGACGGATTCGAGTGCCCGGGAAGTGGGCGTGGGTAATCTGCGC

gi|171365      1300 L  D  L  P  V  D  E  D  D  Y  L  M  P  T  C  Q  P  G  P  N  
gi|246571      3922 CTCGATCTACCAGTCGATGAGGATGATTACCTGATGCCCACATGCCAACCGGGGCCCAAC

gi|171365      1320 N  N  N  N  I  N  N  P  N  Q  N  N  M  A  A  V  G  V  A  A  
gi|246571      3982 AACAACAACAACATAAATAATCCCAATCAAAACAATATGGCAGCTGTGGGCGTGGCTGCC

gi|171365      1340 G  Y  M  D  L  I  G  V  P  V  S  V  D  N  P  E  Y  L  L  N  
gi|246571      4042 GGCTACATGGATCTCATCGGAGTGCCCGTTAGTGTGGACAATCCGGAGTATCTGCTAAAC

gi|171365      1360 A  Q  T  L  G  V  G  E  S  P  I  P  T  Q  T  I  G  I  P  V  
gi|246571      4102 GCGCAGACACTGGGTGTTGGGGAGTCGCCGATACCCACCCAGACCATCGGGATACCGGTG

gi|171365      1380 M  G  V  P  G  T  M  E  V  K  V  P  M  P  G  S  E  P  T  S  
gi|246571      4162 ATGGGAGTCCCGGGCACCATGGAGGTCAAGGTGCCAATGCCAGGCAGTGAGCCAACGAGC

gi|171365      1400 S  D  H  E  Y  Y  N  D  T  Q  R  E  L  Q  P  L  H  R  N  R  
gi|246571      4222 TCCGATCACGAGTACTACAATGATACCCAACGGGAGTTGCAGCCACTGCATCGAAACCGC

gi|171365      1420 N  T  E  T  R  V   1426
gi|246571      4282 AACACGGAGACGAGGGTG 4300
""",
        )
        protein_record = protein_alignment.sequences[2]
        self.assertEqual(protein_record.id, "gi|302179501|gb|ADK98534.1|")
        nucleotide_record = nucleotide_records["gi|302179500|gb|HM749883.1|"]
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1185], [0, 3555]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|302179         0 M  K  K  H  E  L  L  C  Q  G  T  S  N  K  L  T  Q  L  G  T  
gi|302179         0 ATGAAAAAGCACGAGTTACTTTGCCAAGGGACAAGTAACAAGCTCACCCAGTTGGGCACT

gi|302179        20 F  E  D  H  F  L  S  L  Q  R  M  F  N  N  C  E  V  V  L  G  
gi|302179        60 TTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCAACAACTGTGAGGTGGTCCTTGGG

gi|302179        40 N  L  E  I  T  Y  M  Q  S  S  Y  N  L  S  F  L  K  T  I  Q  
gi|302179       120 AATTTGGAAATTACCTACATGCAGAGTAGTTACAACCTTTCTTTTCTCAAGACCATCCAG

gi|302179        60 E  V  A  G  Y  V  L  I  A  L  N  T  V  E  K  I  P  L  E  N  
gi|302179       180 GAGGTTGCCGGCTATGTACTCATTGCCCTCAACACAGTGGAGAAGATTCCGCTGGAAAAC

gi|302179        80 L  Q  I  I  R  G  N  V  L  Y  E  N  T  H  A  L  A  V  L  S  
gi|302179       240 CTGCAGATCATCCGAGGAAATGTGCTTTATGAAAACACCCATGCCTTAGCCGTCTTATCC

gi|302179       100 N  Y  G  A  N  K  T  G  L  R  E  L  P  L  R  N  L  Q  E  I  
gi|302179       300 AACTATGGAGCAAACAAAACCGGACTGAGGGAGCTGCCCTTGAGAAACTTACAGGAAATT

gi|302179       120 L  Q  G  A  V  R  F  S  N  N  P  V  L  C  N  V  E  T  I  Q  
gi|302179       360 CTGCAAGGTGCCGTGAGATTCAGCAACAACCCTGTCCTCTGCAACGTGGAGACCATCCAG

gi|302179       140 W  R  D  I  V  N  P  D  F  L  S  N  M  T  G  D  F  Q  N  Q  
gi|302179       420 TGGCGGGACATCGTCAACCCTGATTTTCTAAGCAACATGACAGGGGACTTTCAGAACCAG

gi|302179       160 Q  G  N  C  P  K  C  D  P  A  C  L  N  R  S  C  W  G  A  G  
gi|302179       480 CAGGGCAACTGCCCAAAGTGTGATCCAGCCTGTCTCAACAGAAGCTGCTGGGGTGCCGGG

gi|302179       180 E  E  N  C  Q  K  L  T  K  I  I  C  A  Q  Q  C  S  G  R  C  
gi|302179       540 GAGGAGAACTGTCAGAAATTGACCAAAATCATCTGTGCCCAGCAGTGTTCCGGGCGCTGC

gi|302179       200 R  G  R  S  P  S  D  C  C  H  N  Q  C  A  A  G  C  T  G  P  
gi|302179       600 CGTGGCAGGTCCCCCAGTGACTGCTGCCACAACCAGTGTGCCGCTGGCTGCACAGGGCCA

gi|302179       220 R  E  S  D  C  L  V  C  R  R  F  R  D  E  A  T  C  K  D  T  
gi|302179       660 CGGGAGAGCGACTGCCTGGTCTGCCGCAGGTTCCGTGATGAAGCCACCTGCAAGGACACG

gi|302179       240 C  P  P  L  M  L  Y  D  P  T  T  Y  E  M  K  V  N  P  L  G  
gi|302179       720 TGTCCGCCACTCATGCTCTATGACCCTACCACCTACGAAATGAAGGTCAACCCGCTGGGG

gi|302179       260 K  Y  S  F  G  A  T  C  V  K  K  C  P  R  N  Y  V  V  T  D  
gi|302179       780 AAGTACAGCTTTGGCGCCACCTGTGTCAAGAAGTGTCCCCGTAACTACGTGGTGACAGAC

gi|302179       280 H  G  S  C  V  R  A  C  S  S  D  S  Q  E  V  E  E  D  G  V  
gi|302179       840 CACGGCTCCTGCGTCCGCGCCTGCAGTTCTGACAGCCAGGAGGTAGAGGAAGACGGTGTC

gi|302179       300 R  K  C  K  K  C  D  G  P  C  G  K  V  C  N  G  I  G  I  G  
gi|302179       900 CGCAAGTGTAAAAAGTGTGACGGGCCTTGTGGCAAAGTTTGTAACGGAATAGGAATCGGT

gi|302179       320 E  F  K  D  T  L  S  I  N  A  T  N  I  K  H  F  R  N  C  T  
gi|302179       960 GAGTTTAAAGACACACTTTCCATAAATGCTACAAACATTAAACACTTCAGAAACTGCACA

gi|302179       340 S  I  S  G  D  L  H  I  L  P  V  A  F  R  G  D  S  F  T  R  
gi|302179      1020 TCCATCAGTGGAGATCTTCATATCCTGCCAGTAGCATTTAGGGGTGACTCCTTCACACGT

gi|302179       360 T  A  P  L  D  P  K  E  L  D  I  L  R  T  V  K  E  I  T  G  
gi|302179      1080 ACTGCACCTCTGGACCCGAAAGAACTGGACATTCTAAGAACTGTAAAAGAAATAACAGGG

gi|302179       380 F  L  L  I  Q  A  W  P  E  N  R  T  D  L  H  A  F  E  N  L  
gi|302179      1140 TTTTTGCTGATTCAGGCCTGGCCCGAAAACAGGACTGACCTCCATGCTTTTGAGAACCTG

gi|302179       400 E  I  I  R  G  R  T  K  Q  H  G  Q  F  S  L  A  V  V  G  L  
gi|302179      1200 GAAATCATACGTGGCAGAACGAAGCAGCATGGCCAGTTTTCTCTTGCGGTTGTCGGCCTG

gi|302179       420 D  I  T  S  L  G  L  R  S  L  K  E  I  S  D  G  D  V  I  I  
gi|302179      1260 GATATAACATCTTTGGGATTACGCTCCCTCAAGGAGATAAGTGATGGTGATGTGATAATT

gi|302179       440 S  G  N  R  N  L  C  Y  A  D  T  I  R  W  K  K  L  F  G  T  
gi|302179      1320 TCAGGAAATCGAAACTTGTGCTATGCAGATACAATACGCTGGAAAAAACTTTTTGGGACC

gi|302179       460 S  T  Q  K  T  K  I  L  N  N  R  S  E  K  Q  C  K  A  A  G  
gi|302179      1380 TCAACTCAGAAAACCAAAATTTTAAACAACAGGAGTGAAAAACAGTGCAAGGCCGCAGGC

gi|302179       480 H  I  C  H  P  L  C  S  S  E  G  C  W  G  P  G  P  K  Y  C  
gi|302179      1440 CACATCTGTCACCCGCTGTGCTCATCAGAGGGCTGCTGGGGACCGGGACCCAAATACTGC

gi|302179       500 M  S  C  Q  N  F  S  R  G  K  E  C  V  G  K  C  N  I  L  E  
gi|302179      1500 ATGTCCTGCCAGAACTTCAGTCGTGGCAAGGAGTGTGTGGGAAAGTGCAACATTCTAGAG

gi|302179       520 G  E  P  R  E  F  V  E  N  S  E  C  V  Q  C  H  P  E  C  L  
gi|302179      1560 GGAGAGCCCAGAGAATTCGTGGAGAACTCCGAGTGTGTGCAGTGCCATCCAGAATGCCTG

gi|302179       540 P  Q  A  M  N  V  T  C  T  G  R  G  P  G  N  C  V  K  C  A  
gi|302179      1620 CCCCAGGCCATGAACGTGACCTGCACTGGACGCGGACCAGGCAACTGTGTAAAGTGCGCC

gi|302179       560 H  Y  I  D  G  P  H  C  V  K  T  C  P  A  G  V  A  G  E  N  
gi|302179      1680 CACTACATTGATGGCCCTCACTGCGTCAAGACCTGCCCTGCTGGAGTCGCGGGAGAGAAT

gi|302179       580 G  T  L  I  W  K  F  A  D  A  N  H  V  C  L  L  C  H  P  N  
gi|302179      1740 GGCACCCTGATCTGGAAGTTTGCAGATGCCAACCACGTGTGTCTCCTGTGCCACCCCAAC

gi|302179       600 C  T  Y  G  C  E  G  P  G  L  E  G  C  P  Q  K  G  P  K  I  
gi|302179      1800 TGCACCTATGGCTGTGAAGGGCCAGGTCTCGAAGGCTGTCCACAAAAAGGGCCCAAGATC

gi|302179       620 P  S  I  A  T  G  I  V  G  G  L  L  L  V  V  V  L  A  L  S  
gi|302179      1860 CCGTCCATTGCCACGGGCATCGTGGGCGGCCTGCTGCTGGTGGTGGTGCTGGCCCTGAGC

gi|302179       640 V  G  L  F  M  R  R  R  H  I  V  R  K  R  T  L  R  R  L  L  
gi|302179      1920 GTCGGCCTCTTCATGCGCAGGCGCCACATCGTGCGCAAGCGCACACTGCGCCGGCTGCTG

gi|302179       660 Q  E  R  E  L  V  E  P  L  T  P  S  G  E  A  P  N  Q  A  L  
gi|302179      1980 CAGGAGCGTGAGCTCGTGGAGCCTCTGACGCCCAGCGGAGAAGCTCCCAACCAAGCTCTC

gi|302179       680 L  R  I  L  K  E  T  E  F  K  K  V  K  V  L  G  S  G  A  F  
gi|302179      2040 TTGAGGATCCTAAAGGAAACAGAATTCAAGAAGGTCAAGGTGCTGGGCTCGGGAGCATTT

gi|302179       700 G  T  V  Y  K  G  L  W  I  P  E  G  E  K  V  K  I  P  V  A  
gi|302179      2100 GGCACCGTGTACAAGGGACTCTGGATCCCAGAAGGCGAGAAGGTTAAAATTCCTGTAGCT

gi|302179       720 I  K  E  L  R  E  A  T  S  P  K  A  N  K  E  I  L  D  E  A  
gi|302179      2160 ATCAAGGAATTAAGAGAAGCCACATCTCCAAAAGCCAACAAGGAAATTCTTGATGAGGCC

gi|302179       740 Y  V  M  A  S  V  D  N  P  H  V  C  R  L  L  G  I  C  L  T  
gi|302179      2220 TACGTGATGGCCAGTGTGGACAACCCCCATGTGTGCCGCCTCCTGGGCATCTGCCTGACC

gi|302179       760 S  T  V  Q  L  I  T  Q  L  M  P  F  G  C  L  L  D  Y  V  R  
gi|302179      2280 TCCACCGTGCAGCTCATCACACAGCTCATGCCCTTCGGCTGCCTGCTGGACTACGTCCGC

gi|302179       780 E  H  K  D  N  V  G  S  Q  Y  L  L  N  W  C  V  Q  I  A  K  
gi|302179      2340 GAGCACAAGGACAATGTCGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAG

gi|302179       800 G  M  N  Y  L  E  D  R  R  L  V  H  R  D  L  A  A  R  N  V  
gi|302179      2400 GGCATGAATTACCTGGAAGACCGGCGCTTGGTGCATAGGGACCTGGCAGCCAGGAACGTG

gi|302179       820 L  V  K  T  P  Q  H  V  K  I  T  D  F  G  L  A  K  L  L  G  
gi|302179      2460 CTGGTGAAGACGCCGCAGCACGTGAAGATCACAGACTTCGGGCTGGCCAAGCTGCTGGGT

gi|302179       840 A  E  E  K  E  Y  H  A  E  G  G  K  V  P  I  K  W  M  A  L  
gi|302179      2520 GCCGAGGAGAAGGAGTATCATGCAGAAGGAGGCAAGGTCCCTATCAAATGGATGGCTTTG

gi|302179       860 E  S  I  L  H  R  I  Y  T  H  Q  S  D  V  W  S  Y  G  V  T  
gi|302179      2580 GAATCAATTTTACACCGAATTTATACCCATCAGAGTGATGTCTGGAGCTATGGAGTCACT

gi|302179       880 V  W  E  L  M  T  F  G  S  K  P  Y  D  G  I  P  A  S  E  I  
gi|302179      2640 GTTTGGGAGTTGATGACCTTTGGATCCAAGCCTTACGATGGAATCCCTGCGAGTGAGATC

gi|302179       900 S  T  V  L  E  K  G  E  R  L  P  Q  P  P  I  C  T  I  D  V  
gi|302179      2700 TCGACTGTCCTGGAGAAAGGAGAGCGCCTCCCACAGCCACCCATCTGCACCATCGACGTC

gi|302179       920 Y  M  I  M  V  K  C  W  M  I  D  A  D  S  R  P  K  F  R  E  
gi|302179      2760 TACATGATCATGGTCAAGTGCTGGATGATAGATGCAGACAGTCGCCCAAAGTTCCGTGAG

gi|302179       940 L  I  L  E  F  S  K  M  A  R  D  P  Q  R  Y  L  V  I  Q  G  
gi|302179      2820 TTGATCCTTGAATTCTCCAAGATGGCCCGAGACCCCCAGCGCTACCTTGTCATCCAGGGG

gi|302179       960 D  E  R  M  H  L  P  S  P  T  D  S  N  F  Y  R  A  L  M  D  
gi|302179      2880 GACGAGAGAATGCATTTGCCAAGCCCTACGGACTCCAACTTCTACCGCGCCCTGATGGAT

gi|302179       980 E  E  D  M  E  D  V  V  D  A  D  E  Y  L  V  P  Q  Q  G  F  
gi|302179      2940 GAGGAGGACATGGAGGATGTTGTGGATGCCGATGAGTACCTCGTCCCCCAGCAGGGCTTC

gi|302179      1000 F  H  S  P  T  T  S  R  T  P  L  L  S  S  L  S  T  S  S  N  
gi|302179      3000 TTCCACAGCCCCACCACCTCCCGGACACCCCTCCTCAGCTCGCTGAGCACCTCCAGCAAC

gi|302179      1020 T  P  T  V  T  C  V  D  R  N  G  S  Y  P  L  K  E  D  S  F  
gi|302179      3060 ACTCCCACTGTGACTTGCGTTGATAGAAATGGGAGCTACCCTCTCAAGGAAGACAGCTTC

gi|302179      1040 L  Q  R  Y  S  S  D  P  T  G  A  L  I  E  D  S  M  D  D  A  
gi|302179      3120 CTGCAGCGCTACAGCTCAGACCCCACTGGTGCCCTCATCGAGGACAGCATGGACGACGCT

gi|302179      1060 F  L  P  V  P  E  Y  V  N  Q  S  V  P  K  R  P  A  G  S  V  
gi|302179      3180 TTCCTCCCAGTACCCGAATATGTAAACCAATCTGTTCCCAAAAGACCCGCAGGCTCTGTC

gi|302179      1080 Q  N  P  V  Y  H  N  Q  P  L  Y  P  A  P  G  R  D  P  Q  Y  
gi|302179      3240 CAGAACCCTGTCTATCACAATCAGCCTCTATATCCAGCTCCTGGCAGAGACCCTCAGTAC

gi|302179      1100 Q  N  S  L  S  N  A  V  D  N  P  E  Y  L  N  T  T  H  P  A  
gi|302179      3300 CAAAATTCACTCAGCAACGCCGTGGACAACCCTGAGTATCTCAACACCACCCATCCTGCC

gi|302179      1120 C  I  N  G  V  L  D  G  P  A  L  W  A  Q  K  G  S  H  Q  F  
gi|302179      3360 TGTATCAATGGTGTGCTCGACGGCCCTGCCCTCTGGGCTCAGAAGGGCAGTCACCAATTT

gi|302179      1140 S  L  D  N  P  D  Y  Q  Q  A  F  F  P  K  E  A  K  S  N  G  
gi|302179      3420 AGCCTAGACAACCCTGACTACCAGCAGGCCTTCTTTCCCAAGGAAGCCAAGTCGAATGGC

gi|302179      1160 I  F  K  G  P  A  A  E  N  A  E  Y  L  R  A  A  P  A  G  S  
gi|302179      3480 ATCTTTAAGGGGCCTGCAGCTGAAAATGCAGAATACCTGCGGGCAGCACCAGCAGGCAGT

gi|302179      1180 D  F  T  G  A   1185
gi|302179      3540 GACTTTACTGGGGCC 3555
""",
        )
        protein_record = protein_alignment.sequences[3]
        self.assertEqual(protein_record.id, "gi|47522840|ref|NP_999172.1|")
        nucleotide_record = nucleotide_records["gi|47522839|ref|NM_214007.1|"]
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1209], [126, 3753]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|475228         0 M  R  R  S  W  A  G  G  A  A  L  L  A  L  L  A  A  H  F  Q  
gi|475228       126 ATGCGACGCTCCTGGGCGGGCGGCGCCGCGCTCCTGGCGCTGCTGGCCGCGCACTTCCAG

gi|475228        20 A  S  P  A  L  E  E  K  K  V  C  Q  G  T  S  N  K  L  T  Q  
gi|475228       186 GCGAGTCCGGCGCTGGAGGAGAAGAAAGTTTGCCAAGGTACAAGTAACAAGCTCACCCAG

gi|475228        40 L  G  T  F  E  D  H  F  L  S  L  Q  R  M  F  N  N  C  E  V  
gi|475228       246 CTGGGCACTTTCGAAGACCACTTTCTGAGCCTCCAGAGGATGTTCAATAACTGCGAGGTG

gi|475228        60 V  L  G  N  L  E  I  T  Y  M  Q  N  S  Y  N  L  S  F  L  K  
gi|475228       306 GTCCTTGGGAACTTGGAGATCACCTACATGCAGAACAGCTACAACCTGTCTTTCCTAAAG

gi|475228        80 T  I  Q  E  V  A  G  Y  V  L  I  A  L  N  T  V  E  K  I  P  
gi|475228       366 ACCATTCAGGAGGTCGCCGGCTACGTGCTCATCGCCCTCAACACCGTGGAGAAGATCCCT

gi|475228       100 L  E  N  L  Q  I  I  R  G  N  V  L  Y  E  N  T  H  A  L  A  
gi|475228       426 TTGGAAAACCTGCAGATCATCCGAGGAAATGTACTGTATGAAAACACCCATGCCTTAGCC

gi|475228       120 V  L  S  N  Y  G  A  N  K  T  G  L  R  E  L  P  M  R  N  L  
gi|475228       486 GTCTTATCCAACTACGGGGCCAATAAAACCGGCCTGAGGGAGCTGCCCATGAGGAACTTA

gi|475228       140 Q  E  I  L  Q  G  A  V  R  F  S  N  N  P  A  L  C  H  A  E  
gi|475228       546 CAAGAGATCCTGCAAGGCGCCGTGCGCTTCAGCAACAACCCTGCCCTCTGTCACGCGGAG

gi|475228       160 S  I  Q  W  R  D  I  V  N  S  D  F  L  S  N  M  S  M  D  F  
gi|475228       606 TCCATCCAGTGGAGGGACATTGTCAACAGCGACTTTCTAAGCAACATGTCCATGGACTTT

gi|475228       180 Q  S  Q  L  G  S  C  P  K  C  D  P  G  C  L  N  G  S  C  W  
gi|475228       666 CAGAGCCAGCTGGGCAGCTGCCCGAAGTGTGATCCAGGCTGTCTCAATGGGAGCTGCTGG

gi|475228       200 G  A  G  K  E  N  C  Q  K  L  T  K  V  I  C  A  Q  Q  C  S  
gi|475228       726 GGTGCTGGGAAGGAGAACTGCCAGAAATTGACCAAAGTCATCTGTGCCCAGCAGTGCTCC

gi|475228       220 G  R  C  R  G  R  S  P  S  D  C  C  H  N  Q  C  A  A  G  C  
gi|475228       786 GGGCGCTGCCGCGGCCGGTCGCCCAGTGACTGCTGCCACAACCAGTGCGCCGCTGGCTGC

gi|475228       240 T  G  P  R  E  S  D  C  L  V  C  R  R  F  R  D  E  A  T  C  
gi|475228       846 ACGGGGCCGCGGGAGAGCGACTGCCTGGTTTGCCGCAGATTCCGTGACGAGGCCACCTGC

gi|475228       260 K  D  T  C  P  P  L  M  L  Y  N  P  T  T  Y  Q  M  D  V  N  
gi|475228       906 AAGGACACATGCCCGCCGCTCATGCTCTACAACCCCACCACCTACCAGATGGACGTCAAC

gi|475228       280 P  L  G  K  Y  S  F  G  A  T  C  V  K  K  C  P  R  N  Y  V  
gi|475228       966 CCGCTGGGGAAGTACAGCTTTGGCGCCACCTGTGTCAAGAAGTGCCCTCGTAACTACGTG

gi|475228       300 V  T  D  H  G  S  C  V  R  A  C  S  S  D  S  Y  E  V  E  E  
gi|475228      1026 GTGACAGACCATGGCTCCTGTGTCCGTGCCTGCAGCTCCGACAGCTACGAGGTGGAGGAG

gi|475228       320 D  G  V  R  K  C  K  K  C  D  G  P  C  G  K  V  C  N  G  I  
gi|475228      1086 GACGGCGTCCGCAAGTGTAAAAAGTGTGACGGGCCCTGCGGCAAAGTTTGTAACGGGATA

gi|475228       340 G  I  G  E  F  K  D  T  L  S  I  N  A  T  N  I  K  H  F  R  
gi|475228      1146 GGGATTGGCGAGTTTAAAGACACACTTTCCATAAATGCTACGAATATCAAGCACTTCAGG

gi|475228       360 N  C  T  S  I  S  G  D  L  H  I  L  P  V  A  F  R  G  D  S  
gi|475228      1206 AACTGCACCTCGATCAGCGGAGATCTTCATATCCTGCCGGTAGCATTTAGGGGTGACTCC

gi|475228       380 F  T  R  T  P  P  L  D  P  K  E  L  D  I  L  K  T  V  K  E  
gi|475228      1266 TTCACACGCACGCCGCCTCTGGACCCCAAGGAACTGGACATCCTGAAAACCGTGAAGGAA

gi|475228       400 I  T  G  F  L  L  I  Q  A  W  P  E  N  R  T  G  L  H  A  F  
gi|475228      1326 ATAACAGGGTTTTTACTGATTCAGGCCTGGCCTGAAAACAGGACTGGCCTCCATGCTTTT

gi|475228       420 E  N  L  E  I  I  R  G  R  T  K  Q  H  G  Q  F  S  L  A  V  
gi|475228      1386 GAGAACCTGGAAATCATACGTGGCAGGACGAAGCAACATGGTCAGTTTTCCCTCGCGGTT

gi|475228       440 V  G  L  D  I  A  S  L  G  L  R  S  L  K  E  I  S  D  G  D  
gi|475228      1446 GTTGGCCTGGACATAGCGTCCTTGGGGATGCGCTCCCTCAAGGAGATCAGCGACGGAGAC

gi|475228       460 V  I  V  S  G  N  R  N  L  C  Y  A  N  T  I  S  W  K  K  L  
gi|475228      1506 GTGATCGTCTCAGGAAACCGAAACCTGTGCTATGCAAATACAATCAGCTGGAAAAAACTA

gi|475228       480 F  G  T  A  S  Q  K  T  K  I  I  N  N  R  S  E  K  E  C  K  
gi|475228      1566 TTTGGGACCGCAAGTCAGAAAACCAAAATTATAAACAACAGGAGCGAAAAAGAGTGCAAA

gi|475228       500 A  M  G  H  I  C  N  P  L  C  S  S  E  G  C  W  G  P  E  P  
gi|475228      1626 GCCATGGGCCACATCTGTAACCCGCTGTGCTCATCAGAGGGCTGCTGGGGCCCTGAACCC

gi|475228       520 R  D  C  M  S  C  R  N  F  S  R  G  K  E  C  V  E  K  C  N  
gi|475228      1686 AGAGACTGCATGTCCTGTCGAAACTTTAGCCGCGGCAAGGAATGTGTGGAGAAGTGCAAC

gi|475228       540 V  L  E  G  E  P  R  E  F  V  E  N  A  E  C  V  Q  C  H  P  
gi|475228      1746 GTTCTGGAGGGGGAGCCGAGAGAGTTCGTGGAGAATGCCGAGTGTGTGCAGTGCCACCCG

gi|475228       560 E  C  L  P  Q  A  K  N  V  T  C  M  G  R  G  P  D  S  C  V  
gi|475228      1806 GAGTGCCTGCCCCAGGCCAAGAACGTGACCTGCATGGGACGCGGACCGGACAGCTGTGTC

gi|475228       580 R  C  A  H  Y  I  D  G  P  H  C  V  K  T  C  P  A  G  I  A  
gi|475228      1866 CGGTGTGCTCACTACATCGACGGCCCTCACTGTGTCAAGACCTGCCCCGCGGGAATCGCA

gi|475228       600 G  E  N  S  T  L  I  W  K  F  A  D  A  N  H  V  C  H  L  C  
gi|475228      1926 GGAGAAAACAGCACCCTCATCTGGAAGTTTGCGGATGCCAACCACGTGTGTCACCTGTGC

gi|475228       620 H  P  N  C  T  Y  G  C  V  G  P  G  L  E  G  C  A  V  D  R  
gi|475228      1986 CACCCCAACTGCACCTACGGCTGTGTCGGACCAGGTCTCGAGGGCTGTGCGGTGGACAGG

gi|475228       640 P  K  I  P  S  I  A  T  G  I  V  G  G  L  L  L  A  V  V  L  
gi|475228      2046 CCCAAGATCCCGTCCATCGCCACCGGGATAGTGGGGGGCCTGCTTCTGGCCGTGGTGCTG

gi|475228       660 A  L  G  V  G  L  F  L  R  R  R  H  I  V  R  K  R  T  L  R  
gi|475228      2106 GCCCTGGGGGTCGGCCTCTTTCTGCGCAGGCGCCACATCGTCCGCAAGCGCACGCTGCGC

gi|475228       680 R  L  L  Q  E  R  E  L  V  E  P  L  T  P  S  G  E  A  P  N  
gi|475228      2166 CGGCTGCTGCAGGAGCGGGAGCTGGTTGAGCCTCTCACACCCAGTGGAGAAGCTCCCAAC

gi|475228       700 Q  A  L  L  R  I  L  K  E  T  E  F  K  K  V  K  V  L  G  S  
gi|475228      2226 CAAGCTCTCTTGAGGATCCTGAAGGAGACGGAATTCAAAAAGGTCAAGGTGCTGGGCTCC

gi|475228       720 G  A  F  G  T  V  Y  K  G  L  W  I  P  E  G  E  K  V  K  I  
gi|475228      2286 GGCGCGTTCGGCACGGTGTACAAGGGCCTCTGGATCCCAGAAGGTGAGAAGGTGAAAATT

gi|475228       740 P  V  A  I  K  E  L  R  E  A  T  S  P  K  A  N  K  E  I  L  
gi|475228      2346 CCTGTGGCTATCAAGGAATTAAGAGAAGCCACTTCTCCAAAAGCCAACAAGGAAATTCTT

gi|475228       760 D  E  A  Y  V  M  A  S  V  D  N  P  H  V  C  R  L  L  G  I  
gi|475228      2406 GACGAAGCCTACGTGATGGCCAGTGTGGACAATCCTCATGTGTGCCGCCTCCTGGGCATC

gi|475228       780 C  L  T  S  T  V  Q  L  I  T  Q  L  M  P  F  G  C  L  L  D  
gi|475228      2466 TGCCTGACCTCCACGGTGCAGCTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGAC

gi|475228       800 Y  V  R  E  H  K  D  N  I  G  S  Q  H  L  L  N  W  C  V  Q  
gi|475228      2526 TACGTCCGCGAGCACAAGGACAACATCGGCTCCCAGCACCTGCTCAACTGGTGTGTGCAG

gi|475228       820 I  A  K  G  M  N  Y  L  E  D  R  R  L  V  H  R  D  L  A  A  
gi|475228      2586 ATCGCAAAGGGCATGAACTATCTGGAAGACCGGCGCTTGGTGCACCGAGACCTGGCGGCC

gi|475228       840 R  N  V  L  V  K  T  P  Q  H  V  K  I  T  D  F  G  L  A  K  
gi|475228      2646 AGGAATGTGCTGGTGAAGACACCGCAGCATGTCAAGATCACTGACTTTGGGCTGGCCAAG

gi|475228       860 L  L  G  A  E  E  K  E  Y  H  A  E  G  G  K  V  P  I  K  W  
gi|475228      2706 CTGCTGGGCGCCGAGGAGAAAGAGTACCACGCGGAAGGAGGCAAAGTGCCCATCAAGTGG

gi|475228       880 L  A  L  E  S  I  L  H  R  V  Y  T  H  Q  S  D  V  W  S  Y  
gi|475228      2766 CTGGCTCTAGAGTCAATCCTGCACCGTGTATACACCCACCAGAGTGACGTCTGGAGCTAC

gi|475228       900 G  V  T  V  W  E  L  M  T  F  G  S  K  P  Y  D  G  I  P  A  
gi|475228      2826 GGAGTCACCGTTTGGGAGCTGATGACCTTTGGGTCCAAGCCTTATGACGGGATCCCCGCG

gi|475228       920 S  E  I  S  T  V  L  E  K  G  E  R  L  P  Q  P  P  I  C  T  
gi|475228      2886 AGTGAGATCTCGACCGTCCTGGAGAAGGGAGAGCGCCTCCCGCAGCCCCCCATCTGCACC

gi|475228       940 I  D  V  Y  M  I  M  V  K  C  W  M  I  D  A  D  S  R  P  K  
gi|475228      2946 ATTGATGTCTACATGATCATGGTCAAGTGCTGGATGATAGATGCTGATAGTCGCCCAAAG

gi|475228       960 F  R  E  L  I  I  E  F  S  K  M  A  R  D  P  Q  R  Y  L  V  
gi|475228      3006 TTCCGTGAGCTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCTACCTTGTC

gi|475228       980 I  Q  G  D  E  R  M  H  L  P  S  P  T  D  S  N  F  Y  R  A  
gi|475228      3066 ATCCAGGGAGACGAGCGAATGCACTTGCCAAGCCCTACGGACTCCAACTTCTACCGCGCC

gi|475228      1000 L  M  D  E  E  D  M  E  D  V  V  D  A  D  E  Y  L  V  P  Q  
gi|475228      3126 CTGATGGACGAGGAGGACATGGAGGATGTGGTGGACGCCGACGAGTACCTCGTCCCCCAG

gi|475228      1020 Q  G  F  F  H  S  P  A  T  S  R  T  P  L  L  S  S  L  S  A  
gi|475228      3186 CAGGGCTTCTTCCACAGCCCCGCCACCTCCCGGACGCCGCTGCTCAGCTCTCTGAGCGCC

gi|475228      1040 T  S  S  T  P  A  V  A  C  V  D  R  N  G  Q  S  Y  P  L  K  
gi|475228      3246 ACCAGCAGCACCCCCGCTGTGGCTTGCGTTGACAGAAACGGGCAGAGTTATCCCCTCAAG

gi|475228      1060 E  D  S  F  L  Q  R  Y  S  S  D  P  T  G  A  L  T  E  D  S  
gi|475228      3306 GAAGACAGCTTCCTGCAGCGGTACAGCTCCGACCCCACTGGCGCCCTGACCGAGGACAGC

gi|475228      1080 L  D  D  T  F  L  P  A  P  E  Y  V  N  Q  S  V  P  K  R  P  
gi|475228      3366 CTAGACGACACTTTTCTCCCAGCACCCGAATATGTAAACCAGTCTGTTCCCAAGAGGCCC

gi|475228      1100 A  G  S  V  Q  N  P  V  Y  H  N  Q  P  L  S  A  A  P  G  R  
gi|475228      3426 GCGGGCTCCGTCCAGAACCCTGTCTACCACAATCAGCCTCTCAGTGCAGCTCCTGGCCGG

gi|475228      1120 D  P  H  Y  Q  N  S  H  S  N  A  V  G  N  P  E  Y  L  N  T  
gi|475228      3486 GACCCCCACTACCAGAACTCCCACAGCAATGCCGTGGGCAACCCTGAGTATCTCAACACC

gi|475228      1140 P  R  P  A  C  I  N  G  G  L  D  G  P  A  F  W  A  Q  T  G  
gi|475228      3546 CCCCGCCCCGCCTGCATCAACGGAGGACTGGACGGCCCTGCCTTCTGGGCACAGACAGGC

gi|475228      1160 S  H  Q  I  N  L  D  N  P  D  Y  Q  Q  A  F  F  P  K  E  A  
gi|475228      3606 AGCCACCAGATTAATCTGGACAACCCAGACTACCAGCAGGCCTTCTTCCCCAAGGAAGCC

gi|475228      1180 K  S  N  G  I  C  K  G  P  A  A  E  N  A  E  Y  L  R  A  A  
gi|475228      3666 AAGTCAAACGGCATCTGTAAGGGTCCCGCCGCCGAAAACGCAGAGTACCTAAGGGCGGCA

gi|475228      1200 P  A  S  S  D  L  T  G  A   1209
gi|475228      3726 CCAGCCAGCAGTGACCTTACTGGGGCA 3753
""",
        )
        protein_record = protein_alignment.sequences[4]
        self.assertEqual(protein_record.id, "gi|29725609|ref|NP_005219.2|")
        nucleotide_record = nucleotide_records["gi|41327737|ref|NM_005228.3|"]
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1210], [246, 3876]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|297256         0 M  R  P  S  G  T  A  G  A  A  L  L  A  L  L  A  A  L  C  P  
gi|413277       246 ATGCGACCCTCCGGGACGGCCGGGGCAGCGCTCCTGGCGCTGCTGGCTGCGCTCTGCCCG

gi|297256        20 A  S  R  A  L  E  E  K  K  V  C  Q  G  T  S  N  K  L  T  Q  
gi|413277       306 GCGAGTCGGGCTCTGGAGGAAAAGAAAGTTTGCCAAGGCACGAGTAACAAGCTCACGCAG

gi|297256        40 L  G  T  F  E  D  H  F  L  S  L  Q  R  M  F  N  N  C  E  V  
gi|413277       366 TTGGGCACTTTTGAAGATCATTTTCTCAGCCTCCAGAGGATGTTCAATAACTGTGAGGTG

gi|297256        60 V  L  G  N  L  E  I  T  Y  V  Q  R  N  Y  D  L  S  F  L  K  
gi|413277       426 GTCCTTGGGAATTTGGAAATTACCTATGTGCAGAGGAATTATGATCTTTCCTTCTTAAAG

gi|297256        80 T  I  Q  E  V  A  G  Y  V  L  I  A  L  N  T  V  E  R  I  P  
gi|413277       486 ACCATCCAGGAGGTGGCTGGTTATGTCCTCATTGCCCTCAACACAGTGGAGCGAATTCCT

gi|297256       100 L  E  N  L  Q  I  I  R  G  N  M  Y  Y  E  N  S  Y  A  L  A  
gi|413277       546 TTGGAAAACCTGCAGATCATCAGAGGAAATATGTACTACGAAAATTCCTATGCCTTAGCA

gi|297256       120 V  L  S  N  Y  D  A  N  K  T  G  L  K  E  L  P  M  R  N  L  
gi|413277       606 GTCTTATCTAACTATGATGCAAATAAAACCGGACTGAAGGAGCTGCCCATGAGAAATTTA

gi|297256       140 Q  E  I  L  H  G  A  V  R  F  S  N  N  P  A  L  C  N  V  E  
gi|413277       666 CAGGAAATCCTGCATGGCGCCGTGCGGTTCAGCAACAACCCTGCCCTGTGCAACGTGGAG

gi|297256       160 S  I  Q  W  R  D  I  V  S  S  D  F  L  S  N  M  S  M  D  F  
gi|413277       726 AGCATCCAGTGGCGGGACATAGTCAGCAGTGACTTTCTCAGCAACATGTCGATGGACTTC

gi|297256       180 Q  N  H  L  G  S  C  Q  K  C  D  P  S  C  P  N  G  S  C  W  
gi|413277       786 CAGAACCACCTGGGCAGCTGCCAAAAGTGTGATCCAAGCTGTCCCAATGGGAGCTGCTGG

gi|297256       200 G  A  G  E  E  N  C  Q  K  L  T  K  I  I  C  A  Q  Q  C  S  
gi|413277       846 GGTGCAGGAGAGGAGAACTGCCAGAAACTGACCAAAATCATCTGTGCCCAGCAGTGCTCC

gi|297256       220 G  R  C  R  G  K  S  P  S  D  C  C  H  N  Q  C  A  A  G  C  
gi|413277       906 GGGCGCTGCCGTGGCAAGTCCCCCAGTGACTGCTGCCACAACCAGTGTGCTGCAGGCTGC

gi|297256       240 T  G  P  R  E  S  D  C  L  V  C  R  K  F  R  D  E  A  T  C  
gi|413277       966 ACAGGCCCCCGGGAGAGCGACTGCCTGGTCTGCCGCAAATTCCGAGACGAAGCCACGTGC

gi|297256       260 K  D  T  C  P  P  L  M  L  Y  N  P  T  T  Y  Q  M  D  V  N  
gi|413277      1026 AAGGACACCTGCCCCCCACTCATGCTCTACAACCCCACCACGTACCAGATGGATGTGAAC

gi|297256       280 P  E  G  K  Y  S  F  G  A  T  C  V  K  K  C  P  R  N  Y  V  
gi|413277      1086 CCCGAGGGCAAATACAGCTTTGGTGCCACCTGCGTGAAGAAGTGTCCCCGTAATTATGTG

gi|297256       300 V  T  D  H  G  S  C  V  R  A  C  G  A  D  S  Y  E  M  E  E  
gi|413277      1146 GTGACAGATCACGGCTCGTGCGTCCGAGCCTGTGGGGCCGACAGCTATGAGATGGAGGAA

gi|297256       320 D  G  V  R  K  C  K  K  C  E  G  P  C  R  K  V  C  N  G  I  
gi|413277      1206 GACGGCGTCCGCAAGTGTAAGAAGTGCGAAGGGCCTTGCCGCAAAGTGTGTAACGGAATA

gi|297256       340 G  I  G  E  F  K  D  S  L  S  I  N  A  T  N  I  K  H  F  K  
gi|413277      1266 GGTATTGGTGAATTTAAAGACTCACTCTCCATAAATGCTACGAATATTAAACACTTCAAA

gi|297256       360 N  C  T  S  I  S  G  D  L  H  I  L  P  V  A  F  R  G  D  S  
gi|413277      1326 AACTGCACCTCCATCAGTGGCGATCTCCACATCCTGCCGGTGGCATTTAGGGGTGACTCC

gi|297256       380 F  T  H  T  P  P  L  D  P  Q  E  L  D  I  L  K  T  V  K  E  
gi|413277      1386 TTCACACATACTCCTCCTCTGGATCCACAGGAACTGGATATTCTGAAAACCGTAAAGGAA

gi|297256       400 I  T  G  F  L  L  I  Q  A  W  P  E  N  R  T  D  L  H  A  F  
gi|413277      1446 ATCACAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCTTT

gi|297256       420 E  N  L  E  I  I  R  G  R  T  K  Q  H  G  Q  F  S  L  A  V  
gi|413277      1506 GAGAACCTAGAAATCATACGCGGCAGGACCAAGCAACATGGTCAGTTTTCTCTTGCAGTC

gi|297256       440 V  S  L  N  I  T  S  L  G  L  R  S  L  K  E  I  S  D  G  D  
gi|413277      1566 GTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGATAAGTGATGGAGAT

gi|297256       460 V  I  I  S  G  N  K  N  L  C  Y  A  N  T  I  N  W  K  K  L  
gi|413277      1626 GTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAATACAATAAACTGGAAAAAACTG

gi|297256       480 F  G  T  S  G  Q  K  T  K  I  I  S  N  R  G  E  N  S  C  K  
gi|413277      1686 TTTGGGACCTCCGGTCAGAAAACCAAAATTATAAGCAACAGAGGTGAAAACAGCTGCAAG

gi|297256       500 A  T  G  Q  V  C  H  A  L  C  S  P  E  G  C  W  G  P  E  P  
gi|413277      1746 GCCACAGGCCAGGTCTGCCATGCCTTGTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCC

gi|297256       520 R  D  C  V  S  C  R  N  V  S  R  G  R  E  C  V  D  K  C  N  
gi|413277      1806 AGGGACTGCGTCTCTTGCCGGAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCAAC

gi|297256       540 L  L  E  G  E  P  R  E  F  V  E  N  S  E  C  I  Q  C  H  P  
gi|413277      1866 CTTCTGGAGGGTGAGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATACAGTGCCACCCA

gi|297256       560 E  C  L  P  Q  A  M  N  I  T  C  T  G  R  G  P  D  N  C  I  
gi|413277      1926 GAGTGCCTGCCTCAGGCCATGAACATCACCTGCACAGGACGGGGACCAGACAACTGTATC

gi|297256       580 Q  C  A  H  Y  I  D  G  P  H  C  V  K  T  C  P  A  G  V  M  
gi|413277      1986 CAGTGTGCCCACTACATTGACGGCCCCCACTGCGTCAAGACCTGCCCGGCAGGAGTCATG

gi|297256       600 G  E  N  N  T  L  V  W  K  Y  A  D  A  G  H  V  C  H  L  C  
gi|413277      2046 GGAGAAAACAACACCCTGGTCTGGAAGTACGCAGACGCCGGCCATGTGTGCCACCTGTGC

gi|297256       620 H  P  N  C  T  Y  G  C  T  G  P  G  L  E  G  C  P  T  N  G  
gi|413277      2106 CATCCAAACTGCACCTACGGATGCACTGGGCCAGGTCTTGAAGGCTGTCCAACGAATGGG

gi|297256       640 P  K  I  P  S  I  A  T  G  M  V  G  A  L  L  L  L  L  V  V  
gi|413277      2166 CCTAAGATCCCGTCCATCGCCACTGGGATGGTGGGGGCCCTCCTCTTGCTGCTGGTGGTG

gi|297256       660 A  L  G  I  G  L  F  M  R  R  R  H  I  V  R  K  R  T  L  R  
gi|413277      2226 GCCCTGGGGATCGGCCTCTTCATGCGAAGGCGCCACATCGTTCGGAAGCGCACGCTGCGG

gi|297256       680 R  L  L  Q  E  R  E  L  V  E  P  L  T  P  S  G  E  A  P  N  
gi|413277      2286 AGGCTGCTGCAGGAGAGGGAGCTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAAC

gi|297256       700 Q  A  L  L  R  I  L  K  E  T  E  F  K  K  I  K  V  L  G  S  
gi|413277      2346 CAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCTGGGCTCC

gi|297256       720 G  A  F  G  T  V  Y  K  G  L  W  I  P  E  G  E  K  V  K  I  
gi|413277      2406 GGTGCGTTCGGCACGGTGTATAAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATT

gi|297256       740 P  V  A  I  K  E  L  R  E  A  T  S  P  K  A  N  K  E  I  L  
gi|413277      2466 CCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTC

gi|297256       760 D  E  A  Y  V  M  A  S  V  D  N  P  H  V  C  R  L  L  G  I  
gi|413277      2526 GATGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATC

gi|297256       780 C  L  T  S  T  V  Q  L  I  T  Q  L  M  P  F  G  C  L  L  D  
gi|413277      2586 TGCCTCACCTCCACCGTGCAGCTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGAC

gi|297256       800 Y  V  R  E  H  K  D  N  I  G  S  Q  Y  L  L  N  W  C  V  Q  
gi|413277      2646 TATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAG

gi|297256       820 I  A  K  G  M  N  Y  L  E  D  R  R  L  V  H  R  D  L  A  A  
gi|413277      2706 ATCGCAAAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCC

gi|297256       840 R  N  V  L  V  K  T  P  Q  H  V  K  I  T  D  F  G  L  A  K  
gi|413277      2766 AGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAA

gi|297256       860 L  L  G  A  E  E  K  E  Y  H  A  E  G  G  K  V  P  I  K  W  
gi|413277      2826 CTGCTGGGTGCGGAAGAGAAAGAATACCATGCAGAAGGAGGCAAAGTGCCTATCAAGTGG

gi|297256       880 M  A  L  E  S  I  L  H  R  I  Y  T  H  Q  S  D  V  W  S  Y  
gi|413277      2886 ATGGCATTGGAATCAATTTTACACAGAATCTATACCCACCAGAGTGATGTCTGGAGCTAC

gi|297256       900 G  V  T  V  W  E  L  M  T  F  G  S  K  P  Y  D  G  I  P  A  
gi|413277      2946 GGGGTGACCGTTTGGGAGTTGATGACCTTTGGATCCAAGCCATATGACGGAATCCCTGCC

gi|297256       920 S  E  I  S  S  I  L  E  K  G  E  R  L  P  Q  P  P  I  C  T  
gi|413277      3006 AGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCTCAGCCACCCATATGTACC

gi|297256       940 I  D  V  Y  M  I  M  V  K  C  W  M  I  D  A  D  S  R  P  K  
gi|413277      3066 ATCGATGTCTACATGATCATGGTCAAGTGCTGGATGATAGACGCAGATAGTCGCCCAAAG

gi|297256       960 F  R  E  L  I  I  E  F  S  K  M  A  R  D  P  Q  R  Y  L  V  
gi|413277      3126 TTCCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCTACCTTGTC

gi|297256       980 I  Q  G  D  E  R  M  H  L  P  S  P  T  D  S  N  F  Y  R  A  
gi|413277      3186 ATTCAGGGGGATGAAAGAATGCATTTGCCAAGTCCTACAGACTCCAACTTCTACCGTGCC

gi|297256      1000 L  M  D  E  E  D  M  D  D  V  V  D  A  D  E  Y  L  I  P  Q  
gi|413277      3246 CTGATGGATGAAGAAGACATGGACGACGTGGTGGATGCCGACGAGTACCTCATCCCACAG

gi|297256      1020 Q  G  F  F  S  S  P  S  T  S  R  T  P  L  L  S  S  L  S  A  
gi|413277      3306 CAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGCTCTCTGAGTGCA

gi|297256      1040 T  S  N  N  S  T  V  A  C  I  D  R  N  G  L  Q  S  C  P  I  
gi|413277      3366 ACCAGCAACAATTCCACCGTGGCTTGCATTGATAGAAATGGGCTGCAAAGCTGTCCCATC

gi|297256      1060 K  E  D  S  F  L  Q  R  Y  S  S  D  P  T  G  A  L  T  E  D  
gi|413277      3426 AAGGAAGACAGCTTCTTGCAGCGATACAGCTCAGACCCCACAGGCGCCTTGACTGAGGAC

gi|297256      1080 S  I  D  D  T  F  L  P  V  P  E  Y  I  N  Q  S  V  P  K  R  
gi|413277      3486 AGCATAGACGACACCTTCCTCCCAGTGCCTGAATACATAAACCAGTCCGTTCCCAAAAGG

gi|297256      1100 P  A  G  S  V  Q  N  P  V  Y  H  N  Q  P  L  N  P  A  P  S  
gi|413277      3546 CCCGCTGGCTCTGTGCAGAATCCTGTCTATCACAATCAGCCTCTGAACCCCGCGCCCAGC

gi|297256      1120 R  D  P  H  Y  Q  D  P  H  S  T  A  V  G  N  P  E  Y  L  N  
gi|413277      3606 AGAGACCCACACTACCAGGACCCCCACAGCACTGCAGTGGGCAACCCCGAGTATCTCAAC

gi|297256      1140 T  V  Q  P  T  C  V  N  S  T  F  D  S  P  A  H  W  A  Q  K  
gi|413277      3666 ACTGTCCAGCCCACCTGTGTCAACAGCACATTCGACAGCCCTGCCCACTGGGCCCAGAAA

gi|297256      1160 G  S  H  Q  I  S  L  D  N  P  D  Y  Q  Q  D  F  F  P  K  E  
gi|413277      3726 GGCAGCCACCAAATTAGCCTGGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAA

gi|297256      1180 A  K  P  N  G  I  F  K  G  S  T  A  E  N  A  E  Y  L  R  V  
gi|413277      3786 GCCAAGCCAAATGGCATCTTTAAGGGCTCCACAGCTGAAAATGCAGAATACCTAAGGGTC

gi|297256      1200 A  P  Q  S  S  E  F  I  G  A   1210
gi|413277      3846 GCGCCACAAAGCAGTGAATTTATTGGAGCA 3876
""",
        )
        protein_record = protein_alignment.sequences[5]
        self.assertEqual(protein_record.id, "gi|6478868|gb|AAF14008.1|")
        nucleotide_record = nucleotide_records["gi|6478867|gb|M37394.2|RATEGFR"]
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 1209], [153, 3780]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|647886         0 M  R  P  S  G  T  A  R  T  K  L  L  L  L  L  A  A  L  C  A  
gi|647886       153 ATGCGACCCTCAGGGACTGCGAGAACCAAGCTACTGCTGCTGCTGGCTGCGCTCTGCGCC

gi|647886        20 A  G  G  A  L  E  E  K  K  V  C  Q  G  T  S  N  R  L  T  Q  
gi|647886       213 GCAGGTGGGGCGCTGGAGGAAAAGAAAGTTTGCCAAGGCACAAGTAACAGGCTCACCCAA

gi|647886        40 L  G  T  F  E  D  H  F  L  S  L  Q  R  M  F  N  N  C  E  V  
gi|647886       273 CTAGGCACCTTTGAAGACCACTTTCTGAGCCTCCAGAGGATGTTCAACAACTGTGAAGTG

gi|647886        60 V  L  G  N  L  E  I  T  Y  V  Q  R  N  Y  D  L  S  F  L  K  
gi|647886       333 GTCCTTGGAAACTTGGAAATCACCTATGTGCAAAGGAATTATGACCTTTCCTTCTTAAAG

gi|647886        80 T  I  Q  E  V  A  G  Y  V  L  I  A  L  N  T  V  E  R  I  P  
gi|647886       393 ACCATCCAGGAGGTGGCTGGCTATGTTCTCATTGCCCTGAACACCGTGGAGAGAATCCCT

gi|647886       100 L  E  N  L  Q  I  I  R  G  N  A  L  Y  E  N  T  Y  A  L  A  
gi|647886       453 TTGGAGAACCTGCAGATCATCAGGGGAAATGCTCTCTACGAAAACACCTACGCCTTAGCC

gi|647886       120 V  L  S  N  Y  G  T  N  K  T  G  L  R  E  L  P  M  R  N  L  
gi|647886       513 GTCCTGTCCAACTATGGAACCAACAAAACTGGGCTTAGGGAACTGCCCATGCGGAACTTA

gi|647886       140 Q  E  I  L  I  G  A  V  R  F  S  N  N  P  I  L  C  N  M  E  
gi|647886       573 CAGGAAATTCTGATCGGTGCTGTGCGATTTAGCAACAACCCCATCCTCTGCAATATGGAG

gi|647886       160 T  I  Q  W  R  D  I  V  Q  D  V  F  L  S  N  M  S  M  D  V  
gi|647886       633 ACCATCCAGTGGAGGGACATCGTCCAAGATGTCTTTCTGAGCAACATGTCAATGGACGTA

gi|647886       180 Q  R  H  L  T  G  C  P  K  C  D  P  S  C  P  N  G  S  C  W  
gi|647886       693 CAGCGCCACCTGACGGGCTGCCCGAAATGTGATCCGAGCTGTCCCAATGGAAGCTGCTGG

gi|647886       200 G  R  G  E  E  N  C  Q  K  L  T  K  I  I  C  A  Q  Q  C  S  
gi|647886       753 GGAAGAGGAGAGGAGAACTGCCAGAAATTGACCAAAATCATCTGCGCCCAGCAATGTTCC

gi|647886       220 R  R  C  R  G  R  S  P  S  D  C  C  H  N  Q  C  A  A  G  C  
gi|647886       813 CGGCGTTGTCGTGGCAGGTCCCCTAGCGACTGCTGCCACAACCAGTGTGCCGCAGGGTGT

gi|647886       240 T  G  P  R  E  S  D  C  L  V  C  H  R  F  R  D  E  A  T  C  
gi|647886       873 ACAGGGCCCAGAGAGAGTGACTGTCTGGTCTGCCACAGGTTCCGAGATGAAGCCACGTGC

gi|647886       260 K  D  T  C  P  P  L  M  L  Y  N  P  T  T  Y  Q  M  D  V  N  
gi|647886       933 AAAGACACCTGCCCACCACTCATGCTGTACAACCCCACCACGTACCAGATGGATGTCAAC

gi|647886       280 P  E  G  K  Y  S  F  G  A  T  C  V  K  K  C  P  R  N  Y  V  
gi|647886       993 CCTGAGGGGAAGTACAGCTTTGGTGCCACCTGTGTGAAGAAATGCCCCAGAAACTACGTG

gi|647886       300 V  T  D  H  G  S  C  V  R  A  C  G  P  D  Y  Y  E  V  E  E  
gi|647886      1053 GTGACAGATCACGGCTCGTGTGTCCGGGCCTGTGGGCCAGACTACTATGAAGTAGAAGAA

gi|647886       320 D  G  V  S  K  C  K  K  C  D  G  P  C  R  K  V  C  N  G  I  
gi|647886      1113 GATGGAGTCAGCAAGTGTAAAAAATGTGACGGGCCCTGCCGCAAAGTTTGCAATGGCATA

gi|647886       340 G  I  G  E  F  K  D  T  L  S  I  N  A  T  N  I  K  H  F  K  
gi|647886      1173 GGCATTGGTGAATTTAAAGACACACTCTCCATAAATGCTACAAACATCAAACACTTCAAG

gi|647886       360 Y  C  T  A  I  S  G  D  L  H  I  L  P  V  A  F  K  G  D  S  
gi|647886      1233 TACTGCACTGCCATCAGTGGGGACCTCCACATCCTGCCAGTGGCCTTTAAGGGGGATTCT

gi|647886       380 F  T  R  T  P  P  L  D  P  R  E  L  E  I  L  K  T  V  K  E  
gi|647886      1293 TTCACCCGCACTCCTCCTCTAGACCCACGGGAACTAGAAATTCTCAAAACTGTGAAGGAA

gi|647886       400 I  T  G  F  L  L  I  Q  A  W  P  E  N  W  T  D  L  H  A  F  
gi|647886      1353 ATAACAGGGTTTTTGCTGATTCAGGCTTGGCCTGAAAACTGGACTGACCTCCATGCTTTT

gi|647886       420 E  N  L  E  I  I  R  G  R  T  K  Q  H  G  Q  F  S  L  A  V  
gi|647886      1413 GAGAACCTAGAAATAATTCGTGGCAGAACAAAGCAACATGGTCAGTTTTCTCTGGCGGTT

gi|647886       440 V  G  L  N  I  T  S  L  G  L  R  S  L  K  E  I  S  D  G  D  
gi|647886      1473 GTCGGCCTGAACATAACATCGCTGGGGTTGCGTTCCCTCAAGGAGATCAGTGATGGGGAT

gi|647886       460 V  I  I  S  G  N  R  N  L  C  Y  A  N  T  I  N  W  K  K  L  
gi|647886      1533 GTGATTATTTCTGGGAACCGAAATTTGTGCTACGCAAACACTATAAACTGGAAAAAACTC

gi|647886       480 F  G  T  P  N  Q  K  T  K  I  M  N  N  R  A  E  K  D  C  K  
gi|647886      1593 TTCGGGACGCCCAATCAAAAGACCAAAATCATGAACAACAGAGCTGAAAAGGACTGCAAG

gi|647886       500 A  T  N  H  V  C  N  P  L  C  S  S  E  G  C  W  G  P  E  P  
gi|647886      1653 GCCACGAACCACGTCTGTAATCCTTTATGCTCCTCGGAAGGCTGCTGGGGCCCTGAGCCC

gi|647886       520 T  D  C  V  S  C  Q  N  V  S  R  G  R  E  C  V  D  K  C  N  
gi|647886      1713 ACGGACTGTGTCTCCTGCCAGAATGTGAGCAGAGGCAGGGAGTGCGTGGACAAGTGCAAC

gi|647886       540 I  L  E  G  E  P  R  E  F  V  E  N  S  E  C  I  Q  C  H  P  
gi|647886      1773 ATCCTGGAGGGGGAACCGAGGGAGTTTGTGGAAAATTCTGAATGCATCCAGTGCCATCCA

gi|647886       560 E  C  L  P  Q  T  M  N  I  T  C  T  G  R  G  P  D  N  C  I  
gi|647886      1833 GAATGTCTGCCCCAGACCATGAACATCACCTGTACAGGCCGGGGGCCAGACAACTGCATC

gi|647886       580 K  C  A  H  Y  V  D  G  P  H  C  V  K  T  C  P  S  G  I  M  
gi|647886      1893 AAGTGTGCCCACTATGTTGATGGTCCCCACTGTGTCAAGACCTGCCCTTCGGGCATCATG

gi|647886       600 G  E  N  N  T  L  V  W  K  F  A  D  A  N  N  V  C  H  L  C  
gi|647886      1953 GGGGAGAACAACACCCTGGTCTGGAAGTTTGCAGATGCCAATAACGTCTGCCACCTCTGC

gi|647886       620 H  A  N  C  T  Y  G  C  A  G  P  G  L  K  G  C  Q  Q  P  E  
gi|647886      2013 CATGCAAACTGTACCTATGGATGTGCTGGGCCAGGCCTTAAAGGATGTCAACAACCAGAA

gi|647886       640 G  P  K  I  P  S  I  A  T  G  I  V  G  G  L  L  F  I  V  V  
gi|647886      2073 GGGCCAAAGATCCCATCCATCGCCACTGGGATTGTGGGTGGCCTCCTCTTCATAGTAGTG

gi|647886       660 V  A  L  G  I  G  L  F  M  R  R  R  Q  L  V  R  K  R  T  L  
gi|647886      2133 GTGGCCCTTGGGATCGGCCTCTTCATGCGTCGACGTCAGCTTGTCCGAAAACGTACACTA

gi|647886       680 R  R  L  L  Q  E  R  E  L  V  E  P  L  T  P  S  G  E  A  P  
gi|647886      2193 CGCCGCCTGCTTCAAGAGAGAGAGCTCGTGGAACCTCTCACACCCAGCGGAGAAGCTCCG

gi|647886       700 N  Q  A  H  L  R  I  L  K  E  T  E  F  K  K  I  K  V  L  G  
gi|647886      2253 AACCAAGCCCACTTGAGGATATTAAAGGAAACAGAATTCAAAAAGATCAAAGTTCTGGGT

gi|647886       720 S  G  A  F  G  T  V  Y  K  G  L  W  I  P  E  G  E  K  V  K  
gi|647886      2313 TCAGGAGCATTTGGCACAGTGTATAAGGGTCTCTGGATCCCAGAAGGCGAGAAAGTGAAA

gi|647886       740 I  P  V  A  I  K  E  L  R  E  A  T  S  P  K  A  N  K  E  I  
gi|647886      2373 ATCCCTGTGGCCATCAAGGAGTTAAGAGAAGCCACATCTCCCAAAGCCAACAAGGAAATC

gi|647886       760 L  D  E  A  Y  V  M  A  S  V  D  N  P  H  V  C  R  L  L  G  
gi|647886      2433 CTTGATGAAGCCTACGTGATGGCCAGTGTGGACAACCCTCATGTATGCCGCCTCCTGGGC

gi|647886       780 I  C  L  T  S  T  V  Q  L  I  T  Q  L  M  P  Y  G  C  L  L  
gi|647886      2493 ATCTGTCTGACCTCCACTGTCCAGCTCATTACACAACTCATGCCCTATGGTTGCCTCCTG

gi|647886       800 D  Y  V  R  E  H  K  D  N  I  G  S  Q  Y  L  L  N  W  C  V  
gi|647886      2553 GACTATGTCCGAGAACATAAGGACAACATTGGCTCCCAGTACCTACTCAACTGGTGTGTG

gi|647886       820 Q  I  A  K  G  M  N  Y  L  E  D  R  R  L  V  H  R  D  L  A  
gi|647886      2613 CAGATTGCAAAGGGCATGAACTACCTGGAAGACCGGCGTTTGGTACACCGTGACTTGGCA

gi|647886       840 A  R  N  V  L  V  K  T  P  Q  H  V  K  I  T  D  F  G  L  A  
gi|647886      2673 GCCAGGAATGTACTGGTAAAGACACCACAGCATGTCAAGATCACAGATTTTGGACTGGCC

gi|647886       860 K  L  L  G  A  E  E  K  E  Y  H  A  E  G  G  K  V  P  I  K  
gi|647886      2733 AAACTGCTTGGTGCTGAGGAGAAAGAATACCATGCAGAGGGGGGCAAAGTGCCTATCAAG

gi|647886       880 W  M  A  L  E  S  I  L  H  R  I  Y  T  H  Q  S  D  V  W  S  
gi|647886      2793 TGGATGGCTTTGGAATCAATTTTACACCGAATTTATACACACCAAAGCGACGTCTGGAGC

gi|647886       900 Y  G  V  T  V  W  E  L  M  T  F  G  S  K  P  Y  D  G  I  P  
gi|647886      2853 TATGGAGTCACCGTGTGGGAACTGATGACCTTTGGGTCCAAGCCTTATGATGGGATCCCT

gi|647886       920 A  S  E  I  S  S  I  L  E  K  G  E  R  L  P  Q  P  P  I  C  
gi|647886      2913 GCAAGTGAGATCTCATCCATCCTAGAGAAAGGAGAGCGCCTTCCACAGCCACCTATCTGC

gi|647886       940 T  I  D  V  Y  M  I  M  V  K  C  W  M  I  D  A  D  S  R  P  
gi|647886      2973 ACCATCGACGTCTACATGATCATGGTCAAGTGCTGGATGATAGATGCTGATAGCCGCCCA

gi|647886       960 K  F  R  E  L  I  L  E  F  S  K  M  A  R  D  P  Q  R  Y  L  
gi|647886      3033 AAGTTCCGAGAGTTGATTCTCGAATTCTCCAAAATGGCCAGAGACCCACAGCGCTACCTT

gi|647886       980 V  I  Q  G  D  E  R  M  H  L  P  S  P  T  D  S  N  F  Y  R  
gi|647886      3093 GTTATCCAGGGGGATGAAAGGATGCATTTGCCGAGCCCTACAGACTCCAACTTTTACCGA

gi|647886      1000 A  L  M  E  E  E  D  M  E  D  V  V  D  A  D  E  Y  L  I  P  
gi|647886      3153 GCCCTGATGGAGGAGGAGGACATGGAAGACGTAGTTGATGCTGATGAATACCTCATCCCA

gi|647886      1020 Q  Q  G  F  F  N  S  P  S  T  S  R  T  P  L  L  S  S  L  S  
gi|647886      3213 CAGCAAGGCTTCTTCAACAGCCCATCCACGTCACGGACTCCACTCTTGAGCTCTCTGAGT

gi|647886      1040 A  N  S  N  S  S  T  V  A  C  I  N  R  N  G  S  C  R  V  K  
gi|647886      3273 GCAAATAGCAACAGTTCCACTGTGGCTTGCATTAATAGAAATGGGAGCTGCCGTGTCAAA

gi|647886      1060 E  D  A  F  L  Q  R  Y  S  S  D  P  T  S  V  L  T  E  D  N  
gi|647886      3333 GAAGACGCCTTCTTGCAACGGTATAGCTCCGATCCCACCAGCGTCCTGACAGAGGACAAC

gi|647886      1080 I  D  D  T  F  L  P  V  P  E  Y  I  N  Q  S  V  P  K  R  P  
gi|647886      3393 ATAGATGACACATTCCTTCCCGTGCCTGAATATATAAACCAATCTGTTCCCAAGAGGCCG

gi|647886      1100 A  G  S  V  Q  N  P  V  Y  H  N  Q  P  L  H  P  A  P  G  R  
gi|647886      3453 GCTGGCTCTGTGCAGAACCCAGTCTATCACAATCAGCCCCTGCATCCAGCTCCTGGAAGA

gi|647886      1120 D  L  H  Y  Q  N  P  H  S  N  A  V  S  N  P  E  Y  L  N  T  
gi|647886      3513 GACCTGCATTATCAAAATCCCCATAGCAATGCGGTGAGCAACCCTGAGTATCTCAACACT

gi|647886      1140 A  Q  P  T  C  L  S  S  G  F  D  S  S  A  L  W  I  Q  K  G  
gi|647886      3573 GCCCAGCCGACCTGCCTCAGTAGTGGGTTTGACAGCTCTGCCCTCTGGATCCAGAAAGGC

gi|647886      1160 S  H  Q  M  S  L  D  N  P  D  Y  Q  Q  D  F  F  P  K  E  A  
gi|647886      3633 AGCCACCAAATGAGCCTGGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAAGAAGCC

gi|647886      1180 K  P  N  G  I  F  K  G  P  T  A  E  N  A  E  Y  L  R  V  A  
gi|647886      3693 AAGCCGAATGGCATCTTTAAGGGCCCCACAGCTGAAAATGCAGAGTACCTGCGGGTGGCA

gi|647886      1200 P  P  S  S  E  F  S  G  A   1209
gi|647886      3753 CCGCCAAGCAGTGAGTTTAGTGGAGCA 3780
""",
        )
        alignment = protein_alignment.mapall(codon_alignments)
        self.assertTrue(
            np.array_equal(
                alignment.coordinates,
                # fmt: off
            np.array([[  84,  105,  114,  129,  171,  183,  198,  198,  210,
                        234,  366,  369,  492,  507,  531,  531,  741,  741,
                        807,  810, 1038, 1038, 1089, 1089, 1107, 1107, 1161,
                       1161, 1245, 1260, 1281, 1290, 1443, 1446, 1779, 1779,
                       1821, 1821, 1902, 1953, 1986, 2085, 2097, 2151, 2175,
                       2301, 2319, 2319, 2355, 2358, 2373, 2394, 2418, 2559,
                       2610, 2613, 2655, 2661, 3585, 3594, 3642, 3645, 3765,
                       3765, 3777, 3777, 3777, 3777, 3843, 3843, 3858, 3864,
                       3894, 3900, 3921, 3921, 3969, 3975, 4038, 4038, 4215],
                      [  22,   22,   31,   46,   88,  100,  115,  283,  295,
                        319,  451,  454,  577,  592,  616,  616,  826,  826,
                        892,  895, 1123, 1123, 1174, 1174, 1192, 1192, 1246,
                       1246, 1330, 1345, 1366, 1375, 1528, 1531, 1864, 1864,
                       1906, 1906, 1987, 2038, 2071, 2170, 2182, 2236, 2260,
                       2386, 2404, 2404, 2440, 2443, 2458, 2479, 2503, 2644,
                       2695, 2698, 2740, 2746, 3670, 3679, 3727, 3730, 3850,
                       3850, 3862, 3862, 3862, 3862, 3928, 3928, 3943, 3949,
                       3979, 3985, 4006, 4006, 4054, 4060, 4123, 4123, 4300],
                      [   0,    0,    0,   15,   15,   15,   15,   15,   15,
                         15,  147,  147,  270,  270,  294,  312,  522,  525,
                        591,  591,  819,  822,  873,  879,  897,  903,  957,
                        981, 1065, 1065, 1086, 1086, 1239, 1239, 1572, 1578,
                       1620, 1638, 1719, 1719, 1752, 1752, 1764, 1764, 1788,
                       1788, 1806, 1809, 1845, 1845, 1860, 1860, 1884, 1884,
                       1935, 1935, 1977, 1977, 2901, 2901, 2949, 2949, 3069,
                       3081, 3093, 3093, 3093, 3099, 3165, 3183, 3198, 3198,
                       3228, 3228, 3249, 3258, 3306, 3306, 3369, 3378, 3555],
                      [ 126,  126,  126,  141,  183,  183,  198,  198,  210,
                        210,  342,  342,  465,  465,  489,  507,  717,  720,
                        786,  786, 1014, 1017, 1068, 1074, 1092, 1098, 1152,
                       1176, 1260, 1260, 1281, 1281, 1434, 1434, 1767, 1773,
                       1815, 1833, 1914, 1914, 1947, 1947, 1959, 1959, 1983,
                       1983, 2001, 2004, 2040, 2040, 2055, 2055, 2079, 2079,
                       2130, 2130, 2172, 2172, 3096, 3096, 3144, 3144, 3264,
                       3276, 3288, 3288, 3291, 3297, 3363, 3381, 3396, 3396,
                       3426, 3426, 3447, 3456, 3504, 3504, 3567, 3576, 3753],
                      [ 246,  246,  246,  261,  303,  303,  318,  318,  330,
                        330,  462,  462,  585,  585,  609,  627,  837,  840,
                        906,  906, 1134, 1137, 1188, 1194, 1212, 1218, 1272,
                       1296, 1380, 1380, 1401, 1401, 1554, 1554, 1887, 1893,
                       1935, 1953, 2034, 2034, 2067, 2067, 2079, 2079, 2103,
                       2103, 2121, 2124, 2160, 2160, 2175, 2175, 2199, 2199,
                       2250, 2250, 2292, 2292, 3216, 3216, 3264, 3264, 3384,
                       3396, 3408, 3411, 3414, 3420, 3486, 3504, 3519, 3519,
                       3549, 3549, 3570, 3579, 3627, 3627, 3690, 3699, 3876],
                      [ 153,  153,  153,  168,  210,  210,  225,  225,  237,
                        237,  369,  369,  492,  492,  516,  534,  744,  747,
                        813,  813, 1041, 1044, 1095, 1101, 1119, 1125, 1179,
                       1203, 1287, 1287, 1308, 1308, 1461, 1461, 1794, 1800,
                       1842, 1860, 1941, 1941, 1974, 1974, 1986, 1986, 2010,
                       2010, 2028, 2031, 2067, 2070, 2085, 2085, 2109, 2109,
                       2160, 2160, 2202, 2202, 3126, 3126, 3174, 3174, 3294,
                       3306, 3318, 3318, 3318, 3324, 3390, 3408, 3423, 3423,
                       3453, 3453, 3474, 3483, 3531, 3531, 3594, 3603, 3780]])
                # fmt: on
            )
        )
        self.assertEqual(
            format(alignment, "clustal"),
            """\
gi|24657088|ref|NM_057410.3|        ATGATGATTATCAGCATGTGGATGAGCATATCGCGAGGATTGTGGGACAG
gi|24657104|ref|NM_057411.3|        ---------------------ATGCTGCTGCGACGGCGCAACGGCCCCTG
gi|302179500|gb|HM749883.1|         ------------------------------ATGAAAAAGCACGAG-----
gi|47522839|ref|NM_214007.1|        ------------------------------ATGCGACGCTCCTGGGCGGG
gi|41327737|ref|NM_005228.3|        ------------------------------ATGCGACCCTCCGGGACGGC
gi|6478867|gb|M37394.2|RATEGFR      ------------------------------ATGCGACCCTCAGGGACTGC

gi|24657088|ref|NM_057410.3|        CAGCTCCATCTGGTCGGTGCTGCTGATCCTCGCCTGCATGGCATCCATCA
gi|24657104|ref|NM_057411.3|        CCCCTTCCCCCTGCTGCTCCTGCTCCTGGCCCACTGCATTTGCATTTGGC
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        CGGCGCCGCGCTCCTGGCGCTGCTGGCCGCGCACTTC------------C
gi|41327737|ref|NM_005228.3|        CGGGGCAGCGCTCCTGGCGCTGCTGGCTGCGCTCTGC------------C
gi|6478867|gb|M37394.2|RATEGFR      GAGAACCAAGCTACTGCTGCTGCTGGCTGCGCTCTGC------------G

gi|24657088|ref|NM_057410.3|        CCACAAGCTCATCG------------------------------------
gi|24657104|ref|NM_057411.3|        CCGCGTCGGCGGCCCGCGATCGCTACGCCCGCCAGAACAATCGCCAGCGC
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        AGGCGAGTCCGGCG------------------------------------
gi|41327737|ref|NM_005228.3|        CGGCGAGTCGGGCT------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      CCGCAGGTGGGGCG------------------------------------

gi|24657088|ref|NM_057410.3|        --------------------------------------------------
gi|24657104|ref|NM_057411.3|        CATCAGGATATAGATCGCGATCGGGATCGAGATCGATTCCTATACCGCAG
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        --------------------------------------------------
gi|41327737|ref|NM_005228.3|        --------------------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      --------------------------------------------------

gi|24657088|ref|NM_057410.3|        --------------------------------------------------
gi|24657104|ref|NM_057411.3|        CAGTTCGGCCCAAAATCGACAGAGGGGCGGGGCCAACTTCGCCCTGGGAC
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        --------------------------------------------------
gi|41327737|ref|NM_005228.3|        --------------------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      --------------------------------------------------

gi|24657088|ref|NM_057410.3|        --------------------------------GTCAGCAATGCCGGCTAT
gi|24657104|ref|NM_057411.3|        TGGGAGCCAACGGAGTCACCATTCCCACCAGTCTGGAGGATAAGAACAAG
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        --------------------------------CTGGAGGAGAAG------
gi|41327737|ref|NM_005228.3|        --------------------------------CTGGAGGAAAAG------
gi|6478867|gb|M37394.2|RATEGFR      --------------------------------CTGGAGGAAAAG------

gi|24657088|ref|NM_057410.3|        GTGGATAATGGCAATATGAAAGTCTGCATCGGCACTAAATCTCGGCTCTC
gi|24657104|ref|NM_057411.3|        AACGAGTTCGTCAAGGGGAAAATCTGCATCGGCACTAAATCTCGGCTCTC
gi|302179500|gb|HM749883.1|         ------------------TTACTTTGCCAAGGGACAAGTAACAAGCTCAC
gi|47522839|ref|NM_214007.1|        ------------------AAAGTTTGCCAAGGTACAAGTAACAAGCTCAC
gi|41327737|ref|NM_005228.3|        ------------------AAAGTTTGCCAAGGCACGAGTAACAAGCTCAC
gi|6478867|gb|M37394.2|RATEGFR      ------------------AAAGTTTGCCAAGGCACAAGTAACAGGCTCAC

gi|24657088|ref|NM_057410.3|        CGTGCCCTCCAACAAGGAACATCATTACCGGAACCTCAGAGATCGGTACA
gi|24657104|ref|NM_057411.3|        CGTGCCCTCCAACAAGGAACATCATTACCGGAACCTCAGAGATCGGTACA
gi|302179500|gb|HM749883.1|         CCAGTTGGGCACTTTTGAAGACCACTTTCTGAGCCTACAGAGGATGTTCA
gi|47522839|ref|NM_214007.1|        CCAGCTGGGCACTTTCGAAGACCACTTTCTGAGCCTCCAGAGGATGTTCA
gi|41327737|ref|NM_005228.3|        GCAGTTGGGCACTTTTGAAGATCATTTTCTCAGCCTCCAGAGGATGTTCA
gi|6478867|gb|M37394.2|RATEGFR      CCAACTAGGCACCTTTGAAGACCACTTTCTGAGCCTCCAGAGGATGTTCA

gi|24657088|ref|NM_057410.3|        CGAACTGTACGTATGTGGATGGCAACCTGGAGCTGACCTGGCTGCCCAAC
gi|24657104|ref|NM_057411.3|        CGAACTGTACGTATGTGGATGGCAACCTGGAGCTGACCTGGCTGCCCAAC
gi|302179500|gb|HM749883.1|         ACAACTGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTACATGCAGAGT
gi|47522839|ref|NM_214007.1|        ATAACTGCGAGGTGGTCCTTGGGAACTTGGAGATCACCTACATGCAGAAC
gi|41327737|ref|NM_005228.3|        ATAACTGTGAGGTGGTCCTTGGGAATTTGGAAATTACCTATGTGCAGAGG
gi|6478867|gb|M37394.2|RATEGFR      ACAACTGTGAAGTGGTCCTTGGAAACTTGGAAATCACCTATGTGCAAAGG

gi|24657088|ref|NM_057410.3|        GAGAATTTGGACCTCAGCTTCCTGGACAACATACGGGAGGTCACCGGCTA
gi|24657104|ref|NM_057411.3|        GAGAATTTGGACCTCAGCTTCCTGGACAACATACGGGAGGTCACCGGCTA
gi|302179500|gb|HM749883.1|         ---AGTTACAACCTTTCTTTTCTCAAGACCATCCAGGAGGTTGCCGGCTA
gi|47522839|ref|NM_214007.1|        ---AGCTACAACCTGTCTTTCCTAAAGACCATTCAGGAGGTCGCCGGCTA
gi|41327737|ref|NM_005228.3|        ---AATTATGATCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCTGGTTA
gi|6478867|gb|M37394.2|RATEGFR      ---AATTATGACCTTTCCTTCTTAAAGACCATCCAGGAGGTGGCTGGCTA

gi|24657088|ref|NM_057410.3|        TATTCTGATCAGTCATGTGGACGTTAAGAAAGTGGTATTTCCCAAACTAC
gi|24657104|ref|NM_057411.3|        TATTCTGATCAGTCATGTGGACGTTAAGAAAGTGGTATTTCCCAAACTAC
gi|302179500|gb|HM749883.1|         TGTACTCATTGCCCTCAACACAGTGGAGAAGATTCCGCTGGAAAACCTGC
gi|47522839|ref|NM_214007.1|        CGTGCTCATCGCCCTCAACACCGTGGAGAAGATCCCTTTGGAAAACCTGC
gi|41327737|ref|NM_005228.3|        TGTCCTCATTGCCCTCAACACAGTGGAGCGAATTCCTTTGGAAAACCTGC
gi|6478867|gb|M37394.2|RATEGFR      TGTTCTCATTGCCCTGAACACCGTGGAGAGAATCCCTTTGGAGAACCTGC

gi|24657088|ref|NM_057410.3|        AAATCATTCGCGGACGCACGCTGTTCAGCTTATCCGTGGAGGAGGAGAAG
gi|24657104|ref|NM_057411.3|        AAATCATTCGCGGACGCACGCTGTTCAGCTTATCCGTGGAGGAGGAGAAG
gi|302179500|gb|HM749883.1|         AGATCATCCGAGGAAATGTGCTTTAT---------------GAAAACACC
gi|47522839|ref|NM_214007.1|        AGATCATCCGAGGAAATGTACTGTAT---------------GAAAACACC
gi|41327737|ref|NM_005228.3|        AGATCATCAGAGGAAATATGTACTAC---------------GAAAATTCC
gi|6478867|gb|M37394.2|RATEGFR      AGATCATCAGGGGAAATGCTCTCTAC---------------GAAAACACC

gi|24657088|ref|NM_057410.3|        TATGCCTTGTTCGTC------------------ACTTATTCCAAAATGTA
gi|24657104|ref|NM_057411.3|        TATGCCTTGTTCGTC------------------ACTTATTCCAAAATGTA
gi|302179500|gb|HM749883.1|         CATGCCTTAGCCGTCTTATCCAACTATGGAGCAAACAAAACCGGACTGAG
gi|47522839|ref|NM_214007.1|        CATGCCTTAGCCGTCTTATCCAACTACGGGGCCAATAAAACCGGCCTGAG
gi|41327737|ref|NM_005228.3|        TATGCCTTAGCAGTCTTATCTAACTATGATGCAAATAAAACCGGACTGAA
gi|6478867|gb|M37394.2|RATEGFR      TACGCCTTAGCCGTCCTGTCCAACTATGGAACCAACAAAACTGGGCTTAG

gi|24657088|ref|NM_057410.3|        CACGCTGGAGATTCCCGATCTACGCGATGTCTTAAATGGCCAAGTGGGCT
gi|24657104|ref|NM_057411.3|        CACGCTGGAGATTCCCGATCTACGCGATGTCTTAAATGGCCAAGTGGGCT
gi|302179500|gb|HM749883.1|         GGAGCTGCCCTTGAGAAACTTACAGGAAATTCTGCAAGGTGCCGTGAGAT
gi|47522839|ref|NM_214007.1|        GGAGCTGCCCATGAGGAACTTACAAGAGATCCTGCAAGGCGCCGTGCGCT
gi|41327737|ref|NM_005228.3|        GGAGCTGCCCATGAGAAATTTACAGGAAATCCTGCATGGCGCCGTGCGGT
gi|6478867|gb|M37394.2|RATEGFR      GGAACTGCCCATGCGGAACTTACAGGAAATTCTGATCGGTGCTGTGCGAT

gi|24657088|ref|NM_057410.3|        TCCACAACAACTACAATCTCTGCCACATGCGAACGATCCAGTGGTCGGAG
gi|24657104|ref|NM_057411.3|        TCCACAACAACTACAATCTCTGCCACATGCGAACGATCCAGTGGTCGGAG
gi|302179500|gb|HM749883.1|         TCAGCAACAACCCTGTCCTCTGCAACGTGGAGACCATCCAGTGGCGGGAC
gi|47522839|ref|NM_214007.1|        TCAGCAACAACCCTGCCCTCTGTCACGCGGAGTCCATCCAGTGGAGGGAC
gi|41327737|ref|NM_005228.3|        TCAGCAACAACCCTGCCCTGTGCAACGTGGAGAGCATCCAGTGGCGGGAC
gi|6478867|gb|M37394.2|RATEGFR      TTAGCAACAACCCCATCCTCTGCAATATGGAGACCATCCAGTGGAGGGAC

gi|24657088|ref|NM_057410.3|        ATTGTATCCAACGGCACGGATGCATACTACAACTACGACTTTACTGCTCC
gi|24657104|ref|NM_057411.3|        ATTGTATCCAACGGCACGGATGCATACTACAACTACGACTTTACTGCTCC
gi|302179500|gb|HM749883.1|         ATCGTCAACCCTGATTTTCTAAGCAACATGACAGGGGACTTTCAGAACCA
gi|47522839|ref|NM_214007.1|        ATTGTCAACAGCGACTTTCTAAGCAACATGTCCATGGACTTTCAGAGCCA
gi|41327737|ref|NM_005228.3|        ATAGTCAGCAGTGACTTTCTCAGCAACATGTCGATGGACTTCCAGAACCA
gi|6478867|gb|M37394.2|RATEGFR      ATCGTCCAAGATGTCTTTCTGAGCAACATGTCAATGGACGTACAGCGCCA

gi|24657088|ref|NM_057410.3|        GGAGCGCGAGTGTCCCAAGTGCCACGAGAGCTGCACGCACGGA---TGTT
gi|24657104|ref|NM_057411.3|        GGAGCGCGAGTGTCCCAAGTGCCACGAGAGCTGCACGCACGGA---TGTT
gi|302179500|gb|HM749883.1|         GCAGGGCAACTGCCCAAAGTGTGATCCAGCCTGTCTCAACAGAAGCTGCT
gi|47522839|ref|NM_214007.1|        GCTGGGCAGCTGCCCGAAGTGTGATCCAGGCTGTCTCAATGGGAGCTGCT
gi|41327737|ref|NM_005228.3|        CCTGGGCAGCTGCCAAAAGTGTGATCCAAGCTGTCCCAATGGGAGCTGCT
gi|6478867|gb|M37394.2|RATEGFR      CCTGACGGGCTGCCCGAAATGTGATCCGAGCTGTCCCAATGGAAGCTGCT

gi|24657088|ref|NM_057410.3|        GGGGCGAGGGTCCCAAGAATTGCCAGAAGTTCAGCAAGCTCACCTGCTCG
gi|24657104|ref|NM_057411.3|        GGGGCGAGGGTCCCAAGAATTGCCAGAAGTTCAGCAAGCTCACCTGCTCG
gi|302179500|gb|HM749883.1|         GGGGTGCCGGGGAGGAGAACTGTCAGAAATTGACCAAAATCATCTGTGCC
gi|47522839|ref|NM_214007.1|        GGGGTGCTGGGAAGGAGAACTGCCAGAAATTGACCAAAGTCATCTGTGCC
gi|41327737|ref|NM_005228.3|        GGGGTGCAGGAGAGGAGAACTGCCAGAAACTGACCAAAATCATCTGTGCC
gi|6478867|gb|M37394.2|RATEGFR      GGGGAAGAGGAGAGGAGAACTGCCAGAAATTGACCAAAATCATCTGCGCC

gi|24657088|ref|NM_057410.3|        CCACAGTGTGCCGGAGGTCGTTGCTATGGACCAAAGCCGCGGGAGTGTTG
gi|24657104|ref|NM_057411.3|        CCACAGTGTGCCGGAGGTCGTTGCTATGGACCAAAGCCGCGGGAGTGTTG
gi|302179500|gb|HM749883.1|         CAGCAGTGTTCC---GGGCGCTGCCGTGGCAGGTCCCCCAGTGACTGCTG
gi|47522839|ref|NM_214007.1|        CAGCAGTGCTCC---GGGCGCTGCCGCGGCCGGTCGCCCAGTGACTGCTG
gi|41327737|ref|NM_005228.3|        CAGCAGTGCTCC---GGGCGCTGCCGTGGCAAGTCCCCCAGTGACTGCTG
gi|6478867|gb|M37394.2|RATEGFR      CAGCAATGTTCC---CGGCGTTGTCGTGGCAGGTCCCCTAGCGACTGCTG

gi|24657088|ref|NM_057410.3|        TCACCTCTTCTGCGCCGGAGGATGCACTGGTCCCACGCAAAAGGATTGCA
gi|24657104|ref|NM_057411.3|        TCACCTCTTCTGCGCCGGAGGATGCACTGGTCCCACGCAAAAGGATTGCA
gi|302179500|gb|HM749883.1|         CCACAACCAGTGTGCCGCTGGCTGCACAGGGCCACGGGAGAGCGACTGCC
gi|47522839|ref|NM_214007.1|        CCACAACCAGTGCGCCGCTGGCTGCACGGGGCCGCGGGAGAGCGACTGCC
gi|41327737|ref|NM_005228.3|        CCACAACCAGTGTGCTGCAGGCTGCACAGGCCCCCGGGAGAGCGACTGCC
gi|6478867|gb|M37394.2|RATEGFR      CCACAACCAGTGTGCCGCAGGGTGTACAGGGCCCAGAGAGAGTGACTGTC

gi|24657088|ref|NM_057410.3|        TCGCCTGCAAGAACTTCTTCGACGAGGGCGTATGCAAGGAGGAATGCCCG
gi|24657104|ref|NM_057411.3|        TCGCCTGCAAGAACTTCTTCGACGAGGGCGTATGCAAGGAGGAATGCCCG
gi|302179500|gb|HM749883.1|         TGGTCTGCCGCAGGTTCCGTGATGAAGCCACCTGCAAGGACACGTGTCCG
gi|47522839|ref|NM_214007.1|        TGGTTTGCCGCAGATTCCGTGACGAGGCCACCTGCAAGGACACATGCCCG
gi|41327737|ref|NM_005228.3|        TGGTCTGCCGCAAATTCCGAGACGAAGCCACGTGCAAGGACACCTGCCCC
gi|6478867|gb|M37394.2|RATEGFR      TGGTCTGCCACAGGTTCCGAGATGAAGCCACGTGCAAAGACACCTGCCCA

gi|24657088|ref|NM_057410.3|        CCCATGCGCAAGTACAATCCCACCACCTATGTTCTTGAAACGAATCCTGA
gi|24657104|ref|NM_057411.3|        CCCATGCGCAAGTACAATCCCACCACCTATGTTCTTGAAACGAATCCTGA
gi|302179500|gb|HM749883.1|         CCACTCATGCTCTATGACCCTACCACCTACGAAATGAAGGTCAACCCGCT
gi|47522839|ref|NM_214007.1|        CCGCTCATGCTCTACAACCCCACCACCTACCAGATGGACGTCAACCCGCT
gi|41327737|ref|NM_005228.3|        CCACTCATGCTCTACAACCCCACCACGTACCAGATGGATGTGAACCCCGA
gi|6478867|gb|M37394.2|RATEGFR      CCACTCATGCTGTACAACCCCACCACGTACCAGATGGATGTCAACCCTGA

gi|24657088|ref|NM_057410.3|        GGGAAAGTATGCCTATGGTGCCACCTGCGTCAAGGAGTGTCCC---GGTC
gi|24657104|ref|NM_057411.3|        GGGAAAGTATGCCTATGGTGCCACCTGCGTCAAGGAGTGTCCC---GGTC
gi|302179500|gb|HM749883.1|         GGGGAAGTACAGCTTTGGCGCCACCTGTGTCAAGAAGTGTCCCCGTAACT
gi|47522839|ref|NM_214007.1|        GGGGAAGTACAGCTTTGGCGCCACCTGTGTCAAGAAGTGCCCTCGTAACT
gi|41327737|ref|NM_005228.3|        GGGCAAATACAGCTTTGGTGCCACCTGCGTGAAGAAGTGTCCCCGTAATT
gi|6478867|gb|M37394.2|RATEGFR      GGGGAAGTACAGCTTTGGTGCCACCTGTGTGAAGAAATGCCCCAGAAACT

gi|24657088|ref|NM_057410.3|        ATCTGTTGCGTGATAATGGCGCCTGCGTGCGCAGCTGTCCCCAGGAC---
gi|24657104|ref|NM_057411.3|        ATCTGTTGCGTGATAATGGCGCCTGCGTGCGCAGCTGTCCCCAGGAC---
gi|302179500|gb|HM749883.1|         ACGTGGTGACAGACCACGGCTCCTGCGTCCGCGCCTGCAGTTCTGACAGC
gi|47522839|ref|NM_214007.1|        ACGTGGTGACAGACCATGGCTCCTGTGTCCGTGCCTGCAGCTCCGACAGC
gi|41327737|ref|NM_005228.3|        ATGTGGTGACAGATCACGGCTCGTGCGTCCGAGCCTGTGGGGCCGACAGC
gi|6478867|gb|M37394.2|RATEGFR      ACGTGGTGACAGATCACGGCTCGTGTGTCCGGGCCTGTGGGCCAGACTAC

gi|24657088|ref|NM_057410.3|        ---AAGATGGACAAGGGGGGC------GAGTGTGTGCCCTGCAATGGACC
gi|24657104|ref|NM_057411.3|        ---AAGATGGACAAGGGGGGC------GAGTGTGTGCCCTGCAATGGACC
gi|302179500|gb|HM749883.1|         CAGGAGGTAGAGGAAGACGGTGTCCGCAAGTGTAAAAAGTGTGACGGGCC
gi|47522839|ref|NM_214007.1|        TACGAGGTGGAGGAGGACGGCGTCCGCAAGTGTAAAAAGTGTGACGGGCC
gi|41327737|ref|NM_005228.3|        TATGAGATGGAGGAAGACGGCGTCCGCAAGTGTAAGAAGTGCGAAGGGCC
gi|6478867|gb|M37394.2|RATEGFR      TATGAAGTAGAAGAAGATGGAGTCAGCAAGTGTAAAAAATGTGACGGGCC

gi|24657088|ref|NM_057410.3|        GTGCCCCAAAACCTGCCCGGGCGTTACTGTC-------------------
gi|24657104|ref|NM_057411.3|        GTGCCCCAAAACCTGCCCGGGCGTTACTGTC-------------------
gi|302179500|gb|HM749883.1|         TTGTGGCAAAGTTTGTAACGGAATAGGAATCGGTGAGTTTAAAGACACAC
gi|47522839|ref|NM_214007.1|        CTGCGGCAAAGTTTGTAACGGGATAGGGATTGGCGAGTTTAAAGACACAC
gi|41327737|ref|NM_005228.3|        TTGCCGCAAAGTGTGTAACGGAATAGGTATTGGTGAATTTAAAGACTCAC
gi|6478867|gb|M37394.2|RATEGFR      CTGCCGCAAAGTTTGCAATGGCATAGGCATTGGTGAATTTAAAGACACAC

gi|24657088|ref|NM_057410.3|        -----CTGCATGCCGGCAACATTGACTCGTTCCGGAATTGTACGGTGATC
gi|24657104|ref|NM_057411.3|        -----CTGCATGCCGGCAACATTGACTCGTTCCGGAATTGTACGGTGATC
gi|302179500|gb|HM749883.1|         TTTCCATAAATGCTACAAACATTAAACACTTCAGAAACTGCACATCCATC
gi|47522839|ref|NM_214007.1|        TTTCCATAAATGCTACGAATATCAAGCACTTCAGGAACTGCACCTCGATC
gi|41327737|ref|NM_005228.3|        TCTCCATAAATGCTACGAATATTAAACACTTCAAAAACTGCACCTCCATC
gi|6478867|gb|M37394.2|RATEGFR      TCTCCATAAATGCTACAAACATCAAACACTTCAAGTACTGCACTGCCATC

gi|24657088|ref|NM_057410.3|        GATGGCAACATTCGCATTTTGGATCAGACCTTCTCGGGCTTCCAGGATGT
gi|24657104|ref|NM_057411.3|        GATGGCAACATTCGCATTTTGGATCAGACCTTCTCGGGCTTCCAGGATGT
gi|302179500|gb|HM749883.1|         AGTGGAGATCTTCATATCCTGCCAGTAGCATTTAGGGGT-----------
gi|47522839|ref|NM_214007.1|        AGCGGAGATCTTCATATCCTGCCGGTAGCATTTAGGGGT-----------
gi|41327737|ref|NM_005228.3|        AGTGGCGATCTCCACATCCTGCCGGTGGCATTTAGGGGT-----------
gi|6478867|gb|M37394.2|RATEGFR      AGTGGGGACCTCCACATCCTGCCAGTGGCCTTTAAGGGG-----------

gi|24657088|ref|NM_057410.3|        CTATGCCAACTACACGATGGGACCACGATACATACCGCTGGATCCCGAGC
gi|24657104|ref|NM_057411.3|        CTATGCCAACTACACGATGGGACCACGATACATACCGCTGGATCCCGAGC
gi|302179500|gb|HM749883.1|         ----GACTCCTTCACACGTACTGCA---------CCTCTGGACCCGAAAG
gi|47522839|ref|NM_214007.1|        ----GACTCCTTCACACGCACGCCG---------CCTCTGGACCCCAAGG
gi|41327737|ref|NM_005228.3|        ----GACTCCTTCACACATACTCCT---------CCTCTGGATCCACAGG
gi|6478867|gb|M37394.2|RATEGFR      ----GATTCTTTCACCCGCACTCCT---------CCTCTAGACCCACGGG

gi|24657088|ref|NM_057410.3|        GACTGGAGGTGTTCTCCACGGTGAAGGAGATCACCGGGTATCTGAATATC
gi|24657104|ref|NM_057411.3|        GACTGGAGGTGTTCTCCACGGTGAAGGAGATCACCGGGTATCTGAATATC
gi|302179500|gb|HM749883.1|         AACTGGACATTCTAAGAACTGTAAAAGAAATAACAGGGTTTTTGCTGATT
gi|47522839|ref|NM_214007.1|        AACTGGACATCCTGAAAACCGTGAAGGAAATAACAGGGTTTTTACTGATT
gi|41327737|ref|NM_005228.3|        AACTGGATATTCTGAAAACCGTAAAGGAAATCACAGGGTTTTTGCTGATT
gi|6478867|gb|M37394.2|RATEGFR      AACTAGAAATTCTCAAAACTGTGAAGGAAATAACAGGGTTTTTGCTGATT

gi|24657088|ref|NM_057410.3|        GAGGGAACCCACCCGCAGTTCCGGAATCTGTCGTACTTCCGCAATCTGGA
gi|24657104|ref|NM_057411.3|        GAGGGAACCCACCCGCAGTTCCGGAATCTGTCGTACTTCCGCAATCTGGA
gi|302179500|gb|HM749883.1|         CAGGCCTGGCCCGAAAACAGGACTGACCTCCATGCTTTTGAGAACCTGGA
gi|47522839|ref|NM_214007.1|        CAGGCCTGGCCTGAAAACAGGACTGGCCTCCATGCTTTTGAGAACCTGGA
gi|41327737|ref|NM_005228.3|        CAGGCTTGGCCTGAAAACAGGACGGACCTCCATGCCTTTGAGAACCTAGA
gi|6478867|gb|M37394.2|RATEGFR      CAGGCTTGGCCTGAAAACTGGACTGACCTCCATGCTTTTGAGAACCTAGA

gi|24657088|ref|NM_057410.3|        AACAATTCATGGCCGCCAGCTGATGGAGAGCATGTTTGCCGCTTTGGCGA
gi|24657104|ref|NM_057411.3|        AACAATTCATGGCCGCCAGCTGATGGAGAGCATGTTTGCCGCTTTGGCGA
gi|302179500|gb|HM749883.1|         AATCATACGTGGCAGAACGAAGCAGCATGGCCAGTTT---TCTCTTGCGG
gi|47522839|ref|NM_214007.1|        AATCATACGTGGCAGGACGAAGCAACATGGTCAGTTT---TCCCTCGCGG
gi|41327737|ref|NM_005228.3|        AATCATACGCGGCAGGACCAAGCAACATGGTCAGTTT---TCTCTTGCAG
gi|6478867|gb|M37394.2|RATEGFR      AATAATTCGTGGCAGAACAAAGCAACATGGTCAGTTT---TCTCTGGCGG

gi|24657088|ref|NM_057410.3|        TCGTTAAGTCATCCCTGTACAGCCTGGAGATGCGCAATCTGAAGCAGATT
gi|24657104|ref|NM_057411.3|        TCGTTAAGTCATCCCTGTACAGCCTGGAGATGCGCAATCTGAAGCAGATT
gi|302179500|gb|HM749883.1|         TTGTCGGCCTGGATATAACATCTTTGGGATTACGCTCCCTCAAGGAGATA
gi|47522839|ref|NM_214007.1|        TTGTTGGCCTGGACATAGCGTCCTTGGGGATGCGCTCCCTCAAGGAGATC
gi|41327737|ref|NM_005228.3|        TCGTCAGCCTGAACATAACATCCTTGGGATTACGCTCCCTCAAGGAGATA
gi|6478867|gb|M37394.2|RATEGFR      TTGTCGGCCTGAACATAACATCGCTGGGGTTGCGTTCCCTCAAGGAGATC

gi|24657088|ref|NM_057410.3|        AGTTCCGGCAGTGTGGTCATCCAGCATAATAGAGACCTCTGCTACGTAAG
gi|24657104|ref|NM_057411.3|        AGTTCCGGCAGTGTGGTCATCCAGCATAATAGAGACCTCTGCTACGTAAG
gi|302179500|gb|HM749883.1|         AGTGATGGTGATGTGATAATTTCAGGAAATCGAAACTTGTGCTATGCAGA
gi|47522839|ref|NM_214007.1|        AGCGACGGAGACGTGATCGTCTCAGGAAACCGAAACCTGTGCTATGCAAA
gi|41327737|ref|NM_005228.3|        AGTGATGGAGATGTGATAATTTCAGGAAACAAAAATTTGTGCTATGCAAA
gi|6478867|gb|M37394.2|RATEGFR      AGTGATGGGGATGTGATTATTTCTGGGAACCGAAATTTGTGCTACGCAAA

gi|24657088|ref|NM_057410.3|        CAATATCCGTTGGCCGGCCATTCAGAAGGAGCCCGAACAGAAGGTGTGGG
gi|24657104|ref|NM_057411.3|        CAATATCCGTTGGCCGGCCATTCAGAAGGAGCCCGAACAGAAGGTGTGGG
gi|302179500|gb|HM749883.1|         TACAATACGCTGGAAAAAACTTTTTGGGACCTCAACTCAGAAAACCAAAA
gi|47522839|ref|NM_214007.1|        TACAATCAGCTGGAAAAAACTATTTGGGACCGCAAGTCAGAAAACCAAAA
gi|41327737|ref|NM_005228.3|        TACAATAAACTGGAAAAAACTGTTTGGGACCTCCGGTCAGAAAACCAAAA
gi|6478867|gb|M37394.2|RATEGFR      CACTATAAACTGGAAAAAACTCTTCGGGACGCCCAATCAAAAGACCAAAA

gi|24657088|ref|NM_057410.3|        TCAACGAGAATCTCAGGGCGGATCTATGCGAGAAAAATGGAACCATTTGC
gi|24657104|ref|NM_057411.3|        TCAACGAGAATCTCAGGGCGGATCTATGCGAGAAAAATGGAACCATTTGC
gi|302179500|gb|HM749883.1|         TTTTAAACAACAGGAGTGAAAAACAGTGCAAGGCCGCAGGCCACATCTGT
gi|47522839|ref|NM_214007.1|        TTATAAACAACAGGAGCGAAAAAGAGTGCAAAGCCATGGGCCACATCTGT
gi|41327737|ref|NM_005228.3|        TTATAAGCAACAGAGGTGAAAACAGCTGCAAGGCCACAGGCCAGGTCTGC
gi|6478867|gb|M37394.2|RATEGFR      TCATGAACAACAGAGCTGAAAAGGACTGCAAGGCCACGAACCACGTCTGT

gi|24657088|ref|NM_057410.3|        TCGGATCAGTGCAACGAGGACGGCTGCTGGGGAGCTGGCACGGATCAGTG
gi|24657104|ref|NM_057411.3|        TCGGATCAGTGCAACGAGGACGGCTGCTGGGGAGCTGGCACGGATCAGTG
gi|302179500|gb|HM749883.1|         CACCCGCTGTGCTCATCAGAGGGCTGCTGGGGACCGGGACCCAAATACTG
gi|47522839|ref|NM_214007.1|        AACCCGCTGTGCTCATCAGAGGGCTGCTGGGGCCCTGAACCCAGAGACTG
gi|41327737|ref|NM_005228.3|        CATGCCTTGTGCTCCCCCGAGGGCTGCTGGGGCCCGGAGCCCAGGGACTG
gi|6478867|gb|M37394.2|RATEGFR      AATCCTTTATGCTCCTCGGAAGGCTGCTGGGGCCCTGAGCCCACGGACTG

gi|24657088|ref|NM_057410.3|        CCTTACCTGCAAGAACTTCAATTTCAATGGCACCTGCATCGCCGACTGTG
gi|24657104|ref|NM_057411.3|        CCTTACCTGCAAGAACTTCAATTTCAATGGCACCTGCATCGCCGACTGTG
gi|302179500|gb|HM749883.1|         CATGTCCTGCCAGAACTTCAGTCGTGGCAAGGAGTGTGTGGGAAAGTGCA
gi|47522839|ref|NM_214007.1|        CATGTCCTGTCGAAACTTTAGCCGCGGCAAGGAATGTGTGGAGAAGTGCA
gi|41327737|ref|NM_005228.3|        CGTCTCTTGCCGGAATGTCAGCCGAGGCAGGGAATGCGTGGACAAGTGCA
gi|6478867|gb|M37394.2|RATEGFR      TGTCTCCTGCCAGAATGTGAGCAGAGGCAGGGAGTGCGTGGACAAGTGCA

gi|24657088|ref|NM_057410.3|        GTTATATATCCAATGCCTACAAG------TTTGACAATAGAACGTGCAAG
gi|24657104|ref|NM_057411.3|        GTTATATATCCAATGCCTACAAG------TTTGACAATAGAACGTGCAAG
gi|302179500|gb|HM749883.1|         ACATTCTAGAGGGAGAGCCCAGAGAATTCGTGGAGAACTCCGAGTGTGTG
gi|47522839|ref|NM_214007.1|        ACGTTCTGGAGGGGGAGCCGAGAGAGTTCGTGGAGAATGCCGAGTGTGTG
gi|41327737|ref|NM_005228.3|        ACCTTCTGGAGGGTGAGCCAAGGGAGTTTGTGGAGAACTCTGAGTGCATA
gi|6478867|gb|M37394.2|RATEGFR      ACATCCTGGAGGGGGAACCGAGGGAGTTTGTGGAAAATTCTGAATGCATC

gi|24657088|ref|NM_057410.3|        ATATGCCATCCAGAGTGCCGG------------------ACTTGCAATGG
gi|24657104|ref|NM_057411.3|        ATATGCCATCCAGAGTGCCGG------------------ACTTGCAATGG
gi|302179500|gb|HM749883.1|         CAGTGCCATCCAGAATGCCTGCCCCAGGCCATGAACGTGACCTGCACTGG
gi|47522839|ref|NM_214007.1|        CAGTGCCACCCGGAGTGCCTGCCCCAGGCCAAGAACGTGACCTGCATGGG
gi|41327737|ref|NM_005228.3|        CAGTGCCACCCAGAGTGCCTGCCTCAGGCCATGAACATCACCTGCACAGG
gi|6478867|gb|M37394.2|RATEGFR      CAGTGCCATCCAGAATGTCTGCCCCAGACCATGAACATCACCTGTACAGG

gi|24657088|ref|NM_057410.3|        AGCTGGAGCAGATCACTGCCAGGAGTGCGTCCATGTGAGGGACGGTCAGC
gi|24657104|ref|NM_057411.3|        AGCTGGAGCAGATCACTGCCAGGAGTGCGTCCATGTGAGGGACGGTCAGC
gi|302179500|gb|HM749883.1|         ACGCGGACCAGGCAACTGTGTAAAGTGCGCCCACTACATTGATGGCCCTC
gi|47522839|ref|NM_214007.1|        ACGCGGACCGGACAGCTGTGTCCGGTGTGCTCACTACATCGACGGCCCTC
gi|41327737|ref|NM_005228.3|        ACGGGGACCAGACAACTGTATCCAGTGTGCCCACTACATTGACGGCCCCC
gi|6478867|gb|M37394.2|RATEGFR      CCGGGGGCCAGACAACTGCATCAAGTGTGCCCACTATGTTGATGGTCCCC

gi|24657088|ref|NM_057410.3|        ACTGTGTGTCCGAGTGCCCGAAGAACAAGTACAACGATCGTGGTGTCTGC
gi|24657104|ref|NM_057411.3|        ACTGTGTGTCCGAGTGCCCGAAGAACAAGTACAACGATCGTGGTGTCTGC
gi|302179500|gb|HM749883.1|         ACTGCGTCAAGACCTGCCCT------------------------------
gi|47522839|ref|NM_214007.1|        ACTGTGTCAAGACCTGCCCC------------------------------
gi|41327737|ref|NM_005228.3|        ACTGCGTCAAGACCTGCCCG------------------------------
gi|6478867|gb|M37394.2|RATEGFR      ACTGTGTCAAGACCTGCCCT------------------------------

gi|24657088|ref|NM_057410.3|        CGAGAGTGCCACGCCACCTGCGATGGATGCACTGGGCCCAAGGACACCAT
gi|24657104|ref|NM_057411.3|        CGAGAGTGCCACGCCACCTGCGATGGATGCACTGGGCCCAAGGACACCAT
gi|302179500|gb|HM749883.1|         ---------------------GCTGGAGTCGCGGGAGAGAATGGCACCCT
gi|47522839|ref|NM_214007.1|        ---------------------GCGGGAATCGCAGGAGAAAACAGCACCCT
gi|41327737|ref|NM_005228.3|        ---------------------GCAGGAGTCATGGGAGAAAACAACACCCT
gi|6478867|gb|M37394.2|RATEGFR      ---------------------TCGGGCATCATGGGGGAGAACAACACCCT

gi|24657088|ref|NM_057410.3|        CGGCATTGGAGCGTGTACAACGTGCAATTTGGCCATTATCAACAATGACG
gi|24657104|ref|NM_057411.3|        CGGCATTGGAGCGTGTACAACGTGCAATTTGGCCATTATCAACAATGACG
gi|302179500|gb|HM749883.1|         GATC----------------------------------------------
gi|47522839|ref|NM_214007.1|        CATC----------------------------------------------
gi|41327737|ref|NM_005228.3|        GGTC----------------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      GGTC----------------------------------------------

gi|24657088|ref|NM_057410.3|        CCACAGTAAAACGCTGCCTGCTGAAGGACGACAAGTGCCCCGATGGGTAC
gi|24657104|ref|NM_057411.3|        CCACAGTAAAACGCTGCCTGCTGAAGGACGACAAGTGCCCCGATGGGTAC
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        --------------------------------------------------
gi|41327737|ref|NM_005228.3|        --------------------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      --------------------------------------------------

gi|24657088|ref|NM_057410.3|        TTCTGGGAGTATGTGCATCCACAAGAGCAGGGATCGCTAAAGCCATTGGC
gi|24657104|ref|NM_057411.3|        TTCTGGGAGTATGTGCATCCACAAGAGCAGGGATCGCTAAAGCCATTGGC
gi|302179500|gb|HM749883.1|         ---TGGAAGTTTGCA-----------------------------------
gi|47522839|ref|NM_214007.1|        ---TGGAAGTTTGCG-----------------------------------
gi|41327737|ref|NM_005228.3|        ---TGGAAGTACGCA-----------------------------------
gi|6478867|gb|M37394.2|RATEGFR      ---TGGAAGTTTGCA-----------------------------------

gi|24657088|ref|NM_057410.3|        CGGCAGAGCAGTTTGCCGAAAGTGCCATCCCCTTTGCGAGCTGTGCACCA
gi|24657104|ref|NM_057411.3|        CGGCAGAGCAGTTTGCCGAAAGTGCCATCCCCTTTGCGAGCTGTGCACCA
gi|302179500|gb|HM749883.1|         -------------------GATGCCAACCACGTGTGTCTCCTG-------
gi|47522839|ref|NM_214007.1|        -------------------GATGCCAACCACGTGTGTCACCTG-------
gi|41327737|ref|NM_005228.3|        -------------------GACGCCGGCCATGTGTGCCACCTG-------
gi|6478867|gb|M37394.2|RATEGFR      -------------------GATGCCAATAACGTCTGCCACCTC-------

gi|24657088|ref|NM_057410.3|        ACTACGGATACCATGAACAGGTGTGCTCCAAGTGCACCCACTACAAGCGA
gi|24657104|ref|NM_057411.3|        ACTACGGATACCATGAACAGGTGTGCTCCAAGTGCACCCACTACAAGCGA
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        --------------------------------------------------
gi|41327737|ref|NM_005228.3|        --------------------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      --------------------------------------------------

gi|24657088|ref|NM_057410.3|        CGAGAGCAGTGCGAGACCGAGTGTCCGGCCGATCACTACACGGATGAGGA
gi|24657104|ref|NM_057411.3|        CGAGAGCAGTGCGAGACCGAGTGTCCGGCCGATCACTACACGGATGAGGA
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        --------------------------------------------------
gi|41327737|ref|NM_005228.3|        --------------------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      --------------------------------------------------

gi|24657088|ref|NM_057410.3|        GCAGCGCGAGTGCTTCCAGTGCCACCCAGAATGCAAC---GGTTGCACTG
gi|24657104|ref|NM_057411.3|        GCAGCGCGAGTGCTTCCAGTGCCACCCAGAATGCAAC---GGTTGCACTG
gi|302179500|gb|HM749883.1|         -------------------TGCCACCCCAACTGCACCTATGGCTGTGAAG
gi|47522839|ref|NM_214007.1|        -------------------TGCCACCCCAACTGCACCTACGGCTGTGTCG
gi|41327737|ref|NM_005228.3|        -------------------TGCCATCCAAACTGCACCTACGGATGCACTG
gi|6478867|gb|M37394.2|RATEGFR      -------------------TGCCATGCAAACTGTACCTATGGATGTGCTG

gi|24657088|ref|NM_057410.3|        GTCCGGGTGCCGACGATTGCAAGTCTTGTCGCAACTTCAAGTTGTTCGAC
gi|24657104|ref|NM_057411.3|        GTCCGGGTGCCGACGATTGCAAGTCTTGTCGCAACTTCAAGTTGTTCGAC
gi|302179500|gb|HM749883.1|         GGCCAGGTCTCGAAGGCTGTCCACAA---AAAGGGCCCAAGATC------
gi|47522839|ref|NM_214007.1|        GACCAGGTCTCGAGGGCTGTGCGGTG---GACAGGCCCAAGATC------
gi|41327737|ref|NM_005228.3|        GGCCAGGTCTTGAAGGCTGTCCAACG---AATGGGCCTAAGATC------
gi|6478867|gb|M37394.2|RATEGFR      GGCCAGGCCTTAAAGGATGTCAACAACCAGAAGGGCCAAAGATC------

gi|24657088|ref|NM_057410.3|        GCGAATGAGACGGGTCCCTATGTGAACTCCACGATGTTCAATTGCACCTC
gi|24657104|ref|NM_057411.3|        GCGAATGAGACGGGTCCCTATGTGAACTCCACGATGTTCAATTGCACCTC
gi|302179500|gb|HM749883.1|         ---------------CCGTCCATTGCCACGGGCATCGTG-----------
gi|47522839|ref|NM_214007.1|        ---------------CCGTCCATCGCCACCGGGATAGTG-----------
gi|41327737|ref|NM_005228.3|        ---------------CCGTCCATCGCCACTGGGATGGTG-----------
gi|6478867|gb|M37394.2|RATEGFR      ---------------CCATCCATCGCCACTGGGATTGTG-----------

gi|24657088|ref|NM_057410.3|        GAAGTGTCCCTTGGAGATGCGACATGTGAACTATCAGTACACGGCCATTG
gi|24657104|ref|NM_057411.3|        GAAGTGTCCCTTGGAGATGCGACATGTGAACTATCAGTACACGGCCATTG
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        --------------------------------------------------
gi|41327737|ref|NM_005228.3|        --------------------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      --------------------------------------------------

gi|24657088|ref|NM_057410.3|        GACCCTACTGTGCAGCTAGTCCGCCGAGGAGCAGCAAGATAACTGCCAAT
gi|24657104|ref|NM_057411.3|        GACCCTACTGTGCAGCTAGTCCGCCGAGGAGCAGCAAGATAACTGCCAAT
gi|302179500|gb|HM749883.1|         --------------------------------------------------
gi|47522839|ref|NM_214007.1|        --------------------------------------------------
gi|41327737|ref|NM_005228.3|        --------------------------------------------------
gi|6478867|gb|M37394.2|RATEGFR      --------------------------------------------------

gi|24657088|ref|NM_057410.3|        CTGGATGTGAACATGATCTTCATTATCACTGGTGCTGTTCTGGTGCCGAC
gi|24657104|ref|NM_057411.3|        CTGGATGTGAACATGATCTTCATTATCACTGGTGCTGTTCTGGTGCCGAC
gi|302179500|gb|HM749883.1|         ------------------------------GGCGGCCTGCTGCTGGTGGT
gi|47522839|ref|NM_214007.1|        ------------------------------GGGGGCCTGCTTCTGGCCGT
gi|41327737|ref|NM_005228.3|        ------------------------------GGGGCCCTCCTCTTGCTGCT
gi|6478867|gb|M37394.2|RATEGFR      ------------------------------GGTGGCCTCCTCTTCATAGT

gi|24657088|ref|NM_057410.3|        GATCTGCATCCTCTGCGTGGTCACATACATTTGTCGGCAAAAGCAAAAGG
gi|24657104|ref|NM_057411.3|        GATCTGCATCCTCTGCGTGGTCACATACATTTGTCGGCAAAAGCAAAAGG
gi|302179500|gb|HM749883.1|         GGTGCTGGCCCTGAGCGTCGGCCTCTTCATG---CGCAGGCGCCACATCG
gi|47522839|ref|NM_214007.1|        GGTGCTGGCCCTGGGGGTCGGCCTCTTTCTG---CGCAGGCGCCACATCG
gi|41327737|ref|NM_005228.3|        GGTGGTGGCCCTGGGGATCGGCCTCTTCATG---CGAAGGCGCCACATCG
gi|6478867|gb|M37394.2|RATEGFR      AGTGGTGGCCCTTGGGATCGGCCTCTTCATG---CGTCGACGTCAGCTTG

gi|24657088|ref|NM_057410.3|        CCAAGAAAGAAACAGTGAAGATGACCATGGCTCTGTCCGGCTGTGAGGAT
gi|24657104|ref|NM_057411.3|        CCAAGAAAGAAACAGTGAAGATGACCATGGCTCTGTCCGGCTGTGAGGAT
gi|302179500|gb|HM749883.1|         TGCGCAAGCGCACACTGCGCCGGCTG------CTGCAGGAGCGTGAGCTC
gi|47522839|ref|NM_214007.1|        TCCGCAAGCGCACGCTGCGCCGGCTG------CTGCAGGAGCGGGAGCTG
gi|41327737|ref|NM_005228.3|        TTCGGAAGCGCACGCTGCGGAGGCTG------CTGCAGGAGAGGGAGCTT
gi|6478867|gb|M37394.2|RATEGFR      TCCGAAAACGTACACTACGCCGCCTG------CTTCAAGAGAGAGAGCTC

gi|24657088|ref|NM_057410.3|        TCCGAGCCGCTGCGTCCCTCGAACATTGGAGCCAATCTATGCAAGTTGCG
gi|24657104|ref|NM_057411.3|        TCCGAGCCGCTGCGTCCCTCGAACATTGGAGCCAATCTATGCAAGTTGCG
gi|302179500|gb|HM749883.1|         GTGGAGCCTCTGACGCCCAGCGGAGAAGCTCCCAACCAAGCTCTCTTGAG
gi|47522839|ref|NM_214007.1|        GTTGAGCCTCTCACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAG
gi|41327737|ref|NM_005228.3|        GTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAG
gi|6478867|gb|M37394.2|RATEGFR      GTGGAACCTCTCACACCCAGCGGAGAAGCTCCGAACCAAGCCCACTTGAG

gi|24657088|ref|NM_057410.3|        CATTGTCAAGGACGCCGAGTTGCGCAAGGGCGGAGTCCTCGGAATGGGAG
gi|24657104|ref|NM_057411.3|        CATTGTCAAGGACGCCGAGTTGCGCAAGGGCGGAGTCCTCGGAATGGGAG
gi|302179500|gb|HM749883.1|         GATCCTAAAGGAAACAGAATTCAAGAAGGTCAAGGTGCTGGGCTCGGGAG
gi|47522839|ref|NM_214007.1|        GATCCTGAAGGAGACGGAATTCAAAAAGGTCAAGGTGCTGGGCTCCGGCG
gi|41327737|ref|NM_005228.3|        GATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCTGGGCTCCGGTG
gi|6478867|gb|M37394.2|RATEGFR      GATATTAAAGGAAACAGAATTCAAAAAGATCAAAGTTCTGGGTTCAGGAG

gi|24657088|ref|NM_057410.3|        CCTTTGGACGAGTGTACAAGGGCGTTTGGGTGCCGGAGGGTGAGAACGTC
gi|24657104|ref|NM_057411.3|        CCTTTGGACGAGTGTACAAGGGCGTTTGGGTGCCGGAGGGTGAGAACGTC
gi|302179500|gb|HM749883.1|         CATTTGGCACCGTGTACAAGGGACTCTGGATCCCAGAAGGCGAGAAGGTT
gi|47522839|ref|NM_214007.1|        CGTTCGGCACGGTGTACAAGGGCCTCTGGATCCCAGAAGGTGAGAAGGTG
gi|41327737|ref|NM_005228.3|        CGTTCGGCACGGTGTATAAGGGACTCTGGATCCCAGAAGGTGAGAAAGTT
gi|6478867|gb|M37394.2|RATEGFR      CATTTGGCACAGTGTATAAGGGTCTCTGGATCCCAGAAGGCGAGAAAGTG

gi|24657088|ref|NM_057410.3|        AAGATTCCAGTGGCCATTAAGGAGCTGCTCAAGTCCACAGGCGCCGAGTC
gi|24657104|ref|NM_057411.3|        AAGATTCCAGTGGCCATTAAGGAGCTGCTCAAGTCCACAGGCGCCGAGTC
gi|302179500|gb|HM749883.1|         AAAATTCCTGTAGCTATCAAGGAATTAAGAGAAGCCACATCTCCAAAAGC
gi|47522839|ref|NM_214007.1|        AAAATTCCTGTGGCTATCAAGGAATTAAGAGAAGCCACTTCTCCAAAAGC
gi|41327737|ref|NM_005228.3|        AAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGC
gi|6478867|gb|M37394.2|RATEGFR      AAAATCCCTGTGGCCATCAAGGAGTTAAGAGAAGCCACATCTCCCAAAGC

gi|24657088|ref|NM_057410.3|        AAGCGAAGAGTTCCTCCGCGAAGCCTACATCATGGCCTCTGTGGAGCACG
gi|24657104|ref|NM_057411.3|        AAGCGAAGAGTTCCTCCGCGAAGCCTACATCATGGCCTCTGTGGAGCACG
gi|302179500|gb|HM749883.1|         CAACAAGGAAATTCTTGATGAGGCCTACGTGATGGCCAGTGTGGACAACC
gi|47522839|ref|NM_214007.1|        CAACAAGGAAATTCTTGACGAAGCCTACGTGATGGCCAGTGTGGACAATC
gi|41327737|ref|NM_005228.3|        CAACAAGGAAATCCTCGATGAAGCCTACGTGATGGCCAGCGTGGACAACC
gi|6478867|gb|M37394.2|RATEGFR      CAACAAGGAAATCCTTGATGAAGCCTACGTGATGGCCAGTGTGGACAACC

gi|24657088|ref|NM_057410.3|        TTAATCTGCTGAAGCTCCTGGCCGTCTGCATGTCCTCACAAATGATGCTA
gi|24657104|ref|NM_057411.3|        TTAATCTGCTGAAGCTCCTGGCCGTCTGCATGTCCTCACAAATGATGCTA
gi|302179500|gb|HM749883.1|         CCCATGTGTGCCGCCTCCTGGGCATCTGCCTGACCTCCACCGTGCAGCTC
gi|47522839|ref|NM_214007.1|        CTCATGTGTGCCGCCTCCTGGGCATCTGCCTGACCTCCACGGTGCAGCTC
gi|41327737|ref|NM_005228.3|        CCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGTGCAGCTC
gi|6478867|gb|M37394.2|RATEGFR      CTCATGTATGCCGCCTCCTGGGCATCTGTCTGACCTCCACTGTCCAGCTC

gi|24657088|ref|NM_057410.3|        ATCACGCAACTGATGCCGCTTGGCTGCCTGTTGGACTATGTGCGAAATAA
gi|24657104|ref|NM_057411.3|        ATCACGCAACTGATGCCGCTTGGCTGCCTGTTGGACTATGTGCGAAATAA
gi|302179500|gb|HM749883.1|         ATCACACAGCTCATGCCCTTCGGCTGCCTGCTGGACTACGTCCGCGAGCA
gi|47522839|ref|NM_214007.1|        ATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGACTACGTCCGCGAGCA
gi|41327737|ref|NM_005228.3|        ATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGACTATGTCCGGGAACA
gi|6478867|gb|M37394.2|RATEGFR      ATTACACAACTCATGCCCTATGGTTGCCTCCTGGACTATGTCCGAGAACA

gi|24657088|ref|NM_057410.3|        CCGGGACAAGATCGGCTCTAAGGCTCTGCTCAACTGGAGCACGCAAATCG
gi|24657104|ref|NM_057411.3|        CCGGGACAAGATCGGCTCTAAGGCTCTGCTCAACTGGAGCACGCAAATCG
gi|302179500|gb|HM749883.1|         CAAGGACAATGTCGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCG
gi|47522839|ref|NM_214007.1|        CAAGGACAACATCGGCTCCCAGCACCTGCTCAACTGGTGTGTGCAGATCG
gi|41327737|ref|NM_005228.3|        CAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCG
gi|6478867|gb|M37394.2|RATEGFR      TAAGGACAACATTGGCTCCCAGTACCTACTCAACTGGTGTGTGCAGATTG

gi|24657088|ref|NM_057410.3|        CCAAGGGCATGTCGTATCTGGAGGAGAAGCGACTGGTCCACAGAGACTTG
gi|24657104|ref|NM_057411.3|        CCAAGGGCATGTCGTATCTGGAGGAGAAGCGACTGGTCCACAGAGACTTG
gi|302179500|gb|HM749883.1|         CAAAGGGCATGAATTACCTGGAAGACCGGCGCTTGGTGCATAGGGACCTG
gi|47522839|ref|NM_214007.1|        CAAAGGGCATGAACTATCTGGAAGACCGGCGCTTGGTGCACCGAGACCTG
gi|41327737|ref|NM_005228.3|        CAAAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTGCACCGCGACCTG
gi|6478867|gb|M37394.2|RATEGFR      CAAAGGGCATGAACTACCTGGAAGACCGGCGTTTGGTACACCGTGACTTG

gi|24657088|ref|NM_057410.3|        GCTGCCCGCAATGTCCTGGTGCAGACTCCCTCGCTGGTGAAGATCACCGA
gi|24657104|ref|NM_057411.3|        GCTGCCCGCAATGTCCTGGTGCAGACTCCCTCGCTGGTGAAGATCACCGA
gi|302179500|gb|HM749883.1|         GCAGCCAGGAACGTGCTGGTGAAGACGCCGCAGCACGTGAAGATCACAGA
gi|47522839|ref|NM_214007.1|        GCGGCCAGGAATGTGCTGGTGAAGACACCGCAGCATGTCAAGATCACTGA
gi|41327737|ref|NM_005228.3|        GCAGCCAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGA
gi|6478867|gb|M37394.2|RATEGFR      GCAGCCAGGAATGTACTGGTAAAGACACCACAGCATGTCAAGATCACAGA

gi|24657088|ref|NM_057410.3|        CTTTGGGCTGGCCAAGTTGCTGAGCAGCGATTCCAATGAGTACAAGGCTG
gi|24657104|ref|NM_057411.3|        CTTTGGGCTGGCCAAGTTGCTGAGCAGCGATTCCAATGAGTACAAGGCTG
gi|302179500|gb|HM749883.1|         CTTCGGGCTGGCCAAGCTGCTGGGTGCCGAGGAGAAGGAGTATCATGCAG
gi|47522839|ref|NM_214007.1|        CTTTGGGCTGGCCAAGCTGCTGGGCGCCGAGGAGAAAGAGTACCACGCGG
gi|41327737|ref|NM_005228.3|        TTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATACCATGCAG
gi|6478867|gb|M37394.2|RATEGFR      TTTTGGACTGGCCAAACTGCTTGGTGCTGAGGAGAAAGAATACCATGCAG

gi|24657088|ref|NM_057410.3|        CTGGCGGCAAGATGCCCATCAAGTGGTTGGCACTGGAGTGCATTCGCAAT
gi|24657104|ref|NM_057411.3|        CTGGCGGCAAGATGCCCATCAAGTGGTTGGCACTGGAGTGCATTCGCAAT
gi|302179500|gb|HM749883.1|         AAGGAGGCAAGGTCCCTATCAAATGGATGGCTTTGGAATCAATTTTACAC
gi|47522839|ref|NM_214007.1|        AAGGAGGCAAAGTGCCCATCAAGTGGCTGGCTCTAGAGTCAATCCTGCAC
gi|41327737|ref|NM_005228.3|        AAGGAGGCAAAGTGCCTATCAAGTGGATGGCATTGGAATCAATTTTACAC
gi|6478867|gb|M37394.2|RATEGFR      AGGGGGGCAAAGTGCCTATCAAGTGGATGGCTTTGGAATCAATTTTACAC

gi|24657088|ref|NM_057410.3|        CGTGTATTCACCAGCAAGTCCGATGTCTGGGCCTTTGGTGTGACAATTTG
gi|24657104|ref|NM_057411.3|        CGTGTATTCACCAGCAAGTCCGATGTCTGGGCCTTTGGTGTGACAATTTG
gi|302179500|gb|HM749883.1|         CGAATTTATACCCATCAGAGTGATGTCTGGAGCTATGGAGTCACTGTTTG
gi|47522839|ref|NM_214007.1|        CGTGTATACACCCACCAGAGTGACGTCTGGAGCTACGGAGTCACCGTTTG
gi|41327737|ref|NM_005228.3|        AGAATCTATACCCACCAGAGTGATGTCTGGAGCTACGGGGTGACCGTTTG
gi|6478867|gb|M37394.2|RATEGFR      CGAATTTATACACACCAAAGCGACGTCTGGAGCTATGGAGTCACCGTGTG

gi|24657088|ref|NM_057410.3|        GGAACTGCTGACCTTTGGCCAGCGTCCACACGAGAACATCCCCGCTAAGG
gi|24657104|ref|NM_057411.3|        GGAACTGCTGACCTTTGGCCAGCGTCCACACGAGAACATCCCCGCTAAGG
gi|302179500|gb|HM749883.1|         GGAGTTGATGACCTTTGGATCCAAGCCTTACGATGGAATCCCTGCGAGTG
gi|47522839|ref|NM_214007.1|        GGAGCTGATGACCTTTGGGTCCAAGCCTTATGACGGGATCCCCGCGAGTG
gi|41327737|ref|NM_005228.3|        GGAGTTGATGACCTTTGGATCCAAGCCATATGACGGAATCCCTGCCAGCG
gi|6478867|gb|M37394.2|RATEGFR      GGAACTGATGACCTTTGGGTCCAAGCCTTATGATGGGATCCCTGCAAGTG

gi|24657088|ref|NM_057410.3|        ATATTCCCGATCTTATTGAAGTCGGTCTGAAGCTGGAGCAGCCGGAGATT
gi|24657104|ref|NM_057411.3|        ATATTCCCGATCTTATTGAAGTCGGTCTGAAGCTGGAGCAGCCGGAGATT
gi|302179500|gb|HM749883.1|         AGATCTCGACTGTCCTGGAGAAAGGAGAGCGCCTCCCACAGCCACCCATC
gi|47522839|ref|NM_214007.1|        AGATCTCGACCGTCCTGGAGAAGGGAGAGCGCCTCCCGCAGCCCCCCATC
gi|41327737|ref|NM_005228.3|        AGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCTCAGCCACCCATA
gi|6478867|gb|M37394.2|RATEGFR      AGATCTCATCCATCCTAGAGAAAGGAGAGCGCCTTCCACAGCCACCTATC

gi|24657088|ref|NM_057410.3|        TGTTCGCTGGACATTTACTGCACACTTCTCTCGTGCTGGCACTTGGATGC
gi|24657104|ref|NM_057411.3|        TGTTCGCTGGACATTTACTGCACACTTCTCTCGTGCTGGCACTTGGATGC
gi|302179500|gb|HM749883.1|         TGCACCATCGACGTCTACATGATCATGGTCAAGTGCTGGATGATAGATGC
gi|47522839|ref|NM_214007.1|        TGCACCATTGATGTCTACATGATCATGGTCAAGTGCTGGATGATAGATGC
gi|41327737|ref|NM_005228.3|        TGTACCATCGATGTCTACATGATCATGGTCAAGTGCTGGATGATAGACGC
gi|6478867|gb|M37394.2|RATEGFR      TGCACCATCGACGTCTACATGATCATGGTCAAGTGCTGGATGATAGATGC

gi|24657088|ref|NM_057410.3|        CGCCATGCGTCCAACCTTCAAGCAGCTGACTACGGTCTTTGCTGAGTTCG
gi|24657104|ref|NM_057411.3|        CGCCATGCGTCCAACCTTCAAGCAGCTGACTACGGTCTTTGCTGAGTTCG
gi|302179500|gb|HM749883.1|         AGACAGTCGCCCAAAGTTCCGTGAGTTGATCCTTGAATTCTCCAAGATGG
gi|47522839|ref|NM_214007.1|        TGATAGTCGCCCAAAGTTCCGTGAGCTGATCATCGAATTCTCCAAAATGG
gi|41327737|ref|NM_005228.3|        AGATAGTCGCCCAAAGTTCCGTGAGTTGATCATCGAATTCTCCAAAATGG
gi|6478867|gb|M37394.2|RATEGFR      TGATAGCCGCCCAAAGTTCCGAGAGTTGATTCTCGAATTCTCCAAAATGG

gi|24657088|ref|NM_057410.3|        CCAGAGATCCGGGTCGCTATCTGGCCATTCCCGGGGATAAGTTCACCCGG
gi|24657104|ref|NM_057411.3|        CCAGAGATCCGGGTCGCTATCTGGCCATTCCCGGGGATAAGTTCACCCGG
gi|302179500|gb|HM749883.1|         CCCGAGACCCCCAGCGCTACCTTGTCATCCAGGGGGACGAGAGAATGCAT
gi|47522839|ref|NM_214007.1|        CCCGAGACCCCCAGCGCTACCTTGTCATCCAGGGAGACGAGCGAATGCAC
gi|41327737|ref|NM_005228.3|        CCCGAGACCCCCAGCGCTACCTTGTCATTCAGGGGGATGAAAGAATGCAT
gi|6478867|gb|M37394.2|RATEGFR      CCAGAGACCCACAGCGCTACCTTGTTATCCAGGGGGATGAAAGGATGCAT

gi|24657088|ref|NM_057410.3|        CTGCCGGCCTACACGAGTCAGGATGAGAAGGATCTCATCCGAAAATTGGC
gi|24657104|ref|NM_057411.3|        CTGCCGGCCTACACGAGTCAGGATGAGAAGGATCTCATCCGAAAATTGGC
gi|302179500|gb|HM749883.1|         TTGCCA---------AGCCCTACGGACTCCAACTTCTACCGCGCCCTGAT
gi|47522839|ref|NM_214007.1|        TTGCCA---------AGCCCTACGGACTCCAACTTCTACCGCGCCCTGAT
gi|41327737|ref|NM_005228.3|        TTGCCA---------AGTCCTACAGACTCCAACTTCTACCGTGCCCTGAT
gi|6478867|gb|M37394.2|RATEGFR      TTGCCG---------AGCCCTACAGACTCCAACTTTTACCGAGCCCTGAT

gi|24657088|ref|NM_057410.3|        TCCCACCACCGATGGGTCCGAAGCCATTGCGGAACCCGATGACTACCTGC
gi|24657104|ref|NM_057411.3|        TCCCACCACCGATGGGTCCGAAGCCATTGCGGAACCCGATGACTACCTGC
gi|302179500|gb|HM749883.1|         GGATGAGGAGGAC---ATGGAGGATGTTGTGGATGCCGATGAGTACCTCG
gi|47522839|ref|NM_214007.1|        GGACGAGGAGGAC---ATGGAGGATGTGGTGGACGCCGACGAGTACCTCG
gi|41327737|ref|NM_005228.3|        GGATGAAGAAGAC---ATGGACGACGTGGTGGATGCCGACGAGTACCTCA
gi|6478867|gb|M37394.2|RATEGFR      GGAGGAGGAGGAC---ATGGAAGACGTAGTTGATGCTGATGAATACCTCA

gi|24657088|ref|NM_057410.3|        AACCCAAGGCAGCACCTGGTCCTAGTCACAGAACCGACTGCACGGATGAG
gi|24657104|ref|NM_057411.3|        AACCCAAGGCAGCACCTGGTCCTAGTCACAGAACCGACTGCACGGATGAG
gi|302179500|gb|HM749883.1|         TCCCCCAGCAGGGCTTCTTCCACAGCCCCACCACCTCCCGGACACCCCTC
gi|47522839|ref|NM_214007.1|        TCCCCCAGCAGGGCTTCTTCCACAGCCCCGCCACCTCCCGGACGCCGCTG
gi|41327737|ref|NM_005228.3|        TCCCACAGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTC
gi|6478867|gb|M37394.2|RATEGFR      TCCCACAGCAAGGCTTCTTCAACAGCCCATCCACGTCACGGACTCCACTC

gi|24657088|ref|NM_057410.3|        ATACCCAAGCTGAACCGCTACTGCAAGGATCCTAGC------------AA
gi|24657104|ref|NM_057411.3|        ATACCCAAGCTGAACCGCTACTGCAAGGATCCTAGC------------AA
gi|302179500|gb|HM749883.1|         CTCAGCTCGCTGAGCACCTCCAGCAACACTCCCACTGTGACTTGCGTTGA
gi|47522839|ref|NM_214007.1|        CTCAGCTCTCTGAGCGCCACCAGCAGCACCCCCGCTGTGGCTTGCGTTGA
gi|41327737|ref|NM_005228.3|        CTGAGCTCTCTGAGTGCAACCAGCAACAATTCCACCGTGGCTTGCATTGA
gi|6478867|gb|M37394.2|RATEGFR      TTGAGCTCTCTGAGTGCAAATAGCAACAGTTCCACTGTGGCTTGCATTAA

gi|24657088|ref|NM_057410.3|        CAAGAATTCG------------AGTACCGGAGACGATGAGACGGATTCGA
gi|24657104|ref|NM_057411.3|        CAAGAATTCG------------AGTACCGGAGACGATGAGACGGATTCGA
gi|302179500|gb|HM749883.1|         TAGAAATGGG------AGCTACCCTCTCAAGGAAGACAGCTTCCTGCAGC
gi|47522839|ref|NM_214007.1|        CAGAAACGGG---CAGAGTTATCCCCTCAAGGAAGACAGCTTCCTGCAGC
gi|41327737|ref|NM_005228.3|        TAGAAATGGGCTGCAAAGCTGTCCCATCAAGGAAGACAGCTTCTTGCAGC
gi|6478867|gb|M37394.2|RATEGFR      TAGAAATGGG------AGCTGCCGTGTCAAAGAAGACGCCTTCTTGCAAC

gi|24657088|ref|NM_057410.3|        GTGCCCGGGAAGTGGGCGTGGGTAATCTGCGCCTCGAT------------
gi|24657104|ref|NM_057411.3|        GTGCCCGGGAAGTGGGCGTGGGTAATCTGCGCCTCGAT------------
gi|302179500|gb|HM749883.1|         GCTACAGCTCAGACCCCACTGGTGCCCTCATCGAGGACAGCATGGACGAC
gi|47522839|ref|NM_214007.1|        GGTACAGCTCCGACCCCACTGGCGCCCTGACCGAGGACAGCCTAGACGAC
gi|41327737|ref|NM_005228.3|        GATACAGCTCAGACCCCACAGGCGCCTTGACTGAGGACAGCATAGACGAC
gi|6478867|gb|M37394.2|RATEGFR      GGTATAGCTCCGATCCCACCAGCGTCCTGACAGAGGACAACATAGATGAC

gi|24657088|ref|NM_057410.3|        ------CTACCAGTCGATGAGGATGATTACCTGATGCCCACATGCCAACC
gi|24657104|ref|NM_057411.3|        ------CTACCAGTCGATGAGGATGATTACCTGATGCCCACATGCCAACC
gi|302179500|gb|HM749883.1|         GCTTTCCTCCCAGTACCCGAA------TATGTAAACCAATCTGTTCCCAA
gi|47522839|ref|NM_214007.1|        ACTTTTCTCCCAGCACCCGAA------TATGTAAACCAGTCTGTTCCCAA
gi|41327737|ref|NM_005228.3|        ACCTTCCTCCCAGTGCCTGAA------TACATAAACCAGTCCGTTCCCAA
gi|6478867|gb|M37394.2|RATEGFR      ACATTCCTTCCCGTGCCTGAA------TATATAAACCAATCTGTTCCCAA

gi|24657088|ref|NM_057410.3|        GGGGCCCAACAACAACAACAACATAAATAATCCC---------AATCAAA
gi|24657104|ref|NM_057411.3|        GGGGCCCAACAACAACAACAACATAAATAATCCC---------AATCAAA
gi|302179500|gb|HM749883.1|         AAGACCC------GCAGGCTCTGTCCAGAACCCTGTCTATCACAATCAGC
gi|47522839|ref|NM_214007.1|        GAGGCCC------GCGGGCTCCGTCCAGAACCCTGTCTACCACAATCAGC
gi|41327737|ref|NM_005228.3|        AAGGCCC------GCTGGCTCTGTGCAGAATCCTGTCTATCACAATCAGC
gi|6478867|gb|M37394.2|RATEGFR      GAGGCCG------GCTGGCTCTGTGCAGAACCCAGTCTATCACAATCAGC

gi|24657088|ref|NM_057410.3|        ACAATATGGCAGCTGTGGGCGTGGCTGCCGGCTACATGGATCTCATCGGA
gi|24657104|ref|NM_057411.3|        ACAATATGGCAGCTGTGGGCGTGGCTGCCGGCTACATGGATCTCATCGGA
gi|302179500|gb|HM749883.1|         CTCTATATCCAGCTCCTGGCAGAGACCCTCAGTACCAAAAT------TCA
gi|47522839|ref|NM_214007.1|        CTCTCAGTGCAGCTCCTGGCCGGGACCCCCACTACCAGAAC------TCC
gi|41327737|ref|NM_005228.3|        CTCTGAACCCCGCGCCCAGCAGAGACCCACACTACCAGGAC------CCC
gi|6478867|gb|M37394.2|RATEGFR      CCCTGCATCCAGCTCCTGGAAGAGACCTGCATTATCAAAAT------CCC

gi|24657088|ref|NM_057410.3|        GTGCCCGTTAGTGTGGACAATCCGGAGTATCTGCTAAACGCGCAGACACT
gi|24657104|ref|NM_057411.3|        GTGCCCGTTAGTGTGGACAATCCGGAGTATCTGCTAAACGCGCAGACACT
gi|302179500|gb|HM749883.1|         CTCAGCAACGCCGTGGACAACCCTGAGTATCTCAACACCACCCATCCTGC
gi|47522839|ref|NM_214007.1|        CACAGCAATGCCGTGGGCAACCCTGAGTATCTCAACACCCCCCGCCCCGC
gi|41327737|ref|NM_005228.3|        CACAGCACTGCAGTGGGCAACCCCGAGTATCTCAACACTGTCCAGCCCAC
gi|6478867|gb|M37394.2|RATEGFR      CATAGCAATGCGGTGAGCAACCCTGAGTATCTCAACACTGCCCAGCCGAC

gi|24657088|ref|NM_057410.3|        GGGTGTTGGG---------GAGTCGCCGATACCCACCCAGACCATCGGGA
gi|24657104|ref|NM_057411.3|        GGGTGTTGGG---------GAGTCGCCGATACCCACCCAGACCATCGGGA
gi|302179500|gb|HM749883.1|         CTGTATCAATGGTGTGCTCGACGGCCCTGCCCTCTGGGCTCAGAAGGGCA
gi|47522839|ref|NM_214007.1|        CTGCATCAACGGAGGACTGGACGGCCCTGCCTTCTGGGCACAGACAGGCA
gi|41327737|ref|NM_005228.3|        CTGTGTCAACAGCACATTCGACAGCCCTGCCCACTGGGCCCAGAAAGGCA
gi|6478867|gb|M37394.2|RATEGFR      CTGCCTCAGTAGTGGGTTTGACAGCTCTGCCCTCTGGATCCAGAAAGGCA

gi|24657088|ref|NM_057410.3|        TACCGGTGATGGGAGTCCCGGGCACCATGGAGGTCAAGGTGCCAATGCCA
gi|24657104|ref|NM_057411.3|        TACCGGTGATGGGAGTCCCGGGCACCATGGAGGTCAAGGTGCCAATGCCA
gi|302179500|gb|HM749883.1|         GTCACCAATTTAGCCTAGACAACCCTGACTACCAGCAGGCCTTCTTTCCC
gi|47522839|ref|NM_214007.1|        GCCACCAGATTAATCTGGACAACCCAGACTACCAGCAGGCCTTCTTCCCC
gi|41327737|ref|NM_005228.3|        GCCACCAAATTAGCCTGGACAACCCTGACTACCAGCAGGACTTCTTTCCC
gi|6478867|gb|M37394.2|RATEGFR      GCCACCAAATGAGCCTGGACAACCCTGACTACCAGCAGGACTTCTTTCCC

gi|24657088|ref|NM_057410.3|        GGCAGTGAGCCAACGAGCTCCGATCACGAGTACTACAATGATACCCAACG
gi|24657104|ref|NM_057411.3|        GGCAGTGAGCCAACGAGCTCCGATCACGAGTACTACAATGATACCCAACG
gi|302179500|gb|HM749883.1|         AAGGAAGCCAAGTCGAATGGCATCTTTAAGGGGCCTGCAGCTGAAAATGC
gi|47522839|ref|NM_214007.1|        AAGGAAGCCAAGTCAAACGGCATCTGTAAGGGTCCCGCCGCCGAAAACGC
gi|41327737|ref|NM_005228.3|        AAGGAAGCCAAGCCAAATGGCATCTTTAAGGGCTCCACAGCTGAAAATGC
gi|6478867|gb|M37394.2|RATEGFR      AAAGAAGCCAAGCCGAATGGCATCTTTAAGGGCCCCACAGCTGAAAATGC

gi|24657088|ref|NM_057410.3|        GGAGTTGCAGCCACTGCATCGAAACCGCAACACGGAGACGAGGGTG
gi|24657104|ref|NM_057411.3|        GGAGTTGCAGCCACTGCATCGAAACCGCAACACGGAGACGAGGGTG
gi|302179500|gb|HM749883.1|         AGAATACCTGCGGGCAGCACCAGCAGGCAGTGACTTTACTGGGGCC
gi|47522839|ref|NM_214007.1|        AGAGTACCTAAGGGCGGCACCAGCCAGCAGTGACCTTACTGGGGCA
gi|41327737|ref|NM_005228.3|        AGAATACCTAAGGGTCGCGCCACAAAGCAGTGAATTTATTGGAGCA
gi|6478867|gb|M37394.2|RATEGFR      AGAGTACCTGCGGGTGGCACCGCCAAGCAGTGAGTTTAGTGGAGCA


""",
        )

        pairwise_alignment = alignment[:2]
        dN, dS = calculate_dn_ds(pairwise_alignment, method="NG86")
        self.assertAlmostEqual(dN, 0.0209, places=4)
        self.assertAlmostEqual(dS, 0.0178, places=4)
        dN, dS = calculate_dn_ds(pairwise_alignment, method="LWL85")
        self.assertAlmostEqual(dN, 0.0203, places=4)
        self.assertAlmostEqual(dS, 0.0164, places=4)

        try:
            import scipy
        except ImportError:
            # Silently skip the rest of the test
            return

        # This should be present:
        from scipy.linalg import expm

        dN, dS = calculate_dn_ds(pairwise_alignment, method="YN00")
        self.assertAlmostEqual(dN, 0.0198, places=4)
        self.assertAlmostEqual(dS, 0.0222, places=4)

        try:
            # New in scipy v0.11
            from scipy.optimize import minimize

            dN, dS = calculate_dn_ds(pairwise_alignment, method="ML")
            self.assertAlmostEqual(dN, 0.0194, places=4)
            self.assertAlmostEqual(dS, 0.0217, places=4)
        except ImportError:
            pass

        # NG86 method with default codon table
        dn_correct = [
            0,
            0.02090783050583131,
            0,
            0.6115239249238438,
            0.6102203266798018,
            0,
            0.6140350835631757,
            0.6040168621204747,
            0.041180350405913294,
            0,
            0.6141532531400524,
            0.6018263135601294,
            0.06701051445629494,
            0.061470360954086874,
            0,
            0.6187088340904762,
            0.6068687248870475,
            0.07386903034833081,
            0.07357890927918581,
            0.05179847072570129,
            0,
        ]
        ds_correct = [
            0,
            0.01783718763890243,
            0,
            2.9382055377913687,
            3.0375115405379267,
            0,
            2.008913071877126,
            2.0182088023715616,
            0.5638033197005285,
            0,
            2.771425931736778,
            2.7353083173058295,
            0.6374483799734671,
            0.723542095485497,
            0,
            -1,
            -1,
            0.953865978141643,
            1.182154857347706,
            0.843182957978177,
            0,
        ]
        dn, ds = calculate_dn_ds_matrix(alignment)
        dn_list = []
        for i in dn.matrix:
            dn_list.extend(i)
        for dn_cal, dn_corr in zip(dn_list, dn_correct):
            self.assertAlmostEqual(dn_cal, dn_corr, places=4)
        ds_list = []
        for i in ds.matrix:
            ds_list.extend(i)
        for ds_cal, ds_corr in zip(ds_list, ds_correct):
            self.assertAlmostEqual(ds_cal, ds_corr, places=4)
        # YN00 method with user specified codon table
        dn_correct = [
            0,
            0.019701773284646867,
            0,
            0.6109649819852769,
            0.6099903856901369,
            0,
            0.6114499930666559,
            0.6028068208599121,
            0.045158286242251426,
            0,
            0.6151835071687592,
            0.6053227393422296,
            0.07034397741651377,
            0.06956967795096626,
            0,
            0.6103850655769698,
            0.5988716898831496,
            0.07905930042150053,
            0.08203052937107111,
            0.05659346894088538,
            0,
        ]
        ds_correct = [
            0,
            0.01881718550096053,
            0,
            1.814457265482046,
            1.8417575124882066,
            0,
            1.5627041719628896,
            1.563930819079887,
            0.4748890153032888,
            0,
            1.6754828466084355,
            1.6531212012501901,
            0.5130923627791538,
            0.5599667707191436,
            0,
            2.0796114236540943,
            2.1452591651827304,
            0.7243066372971764,
            0.8536617406770075,
            0.6509203399899367,
            0,
        ]
        dn, ds = calculate_dn_ds_matrix(
            alignment, method="LWL85", codon_table=CodonTable.unambiguous_dna_by_id[3]
        )
        dn_list = []
        for i in dn.matrix:
            dn_list.extend(i)
        for dn_cal, dn_corr in zip(dn_list, dn_correct):
            self.assertAlmostEqual(dn_cal, dn_corr, places=4)
        ds_list = []
        for i in ds.matrix:
            ds_list.extend(i)
        for ds_cal, ds_corr in zip(ds_list, ds_correct):
            self.assertAlmostEqual(ds_cal, ds_corr, places=4)


class Test_MK(unittest.TestCase):
    def test_mk(self):
        aligner = CodonAligner()
        nucleotide_records = SeqIO.index("codonalign/drosophila.fasta", "fasta")
        protein_alignment = Align.read("codonalign/adh.aln", "clustal")
        self.assertEqual(len(protein_alignment.sequences), 27)
        codon_alignments = []
        protein_record = protein_alignment.sequences[0]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9217|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9217|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9217|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9217|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9217|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9217|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9217|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9217|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9217|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9217|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9217|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9217|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACTGCCATC

gi|9217|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9217|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9217|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9217|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9217|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9217|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9217|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9217|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9217|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9217|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9217|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9217|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9217|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9217|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[1]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9219|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9219|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9219|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9219|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9219|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9219|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9219|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9219|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9219|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9219|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9219|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9219|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACTGCCATC

gi|9219|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9219|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9219|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9219|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9219|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9219|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9219|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9219|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9219|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9219|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9219|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9219|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9219|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9219|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[2]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9221|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9221|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9221|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9221|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGCGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9221|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9221|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9221|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9221|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9221|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9221|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9221|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9221|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACAACGACGGCCATC

gi|9221|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9221|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATAATCTGCAACATTGGATCC

gi|9221|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9221|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9221|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9221|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9221|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9221|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9221|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9221|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9221|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9221|e       660 CAGAACTTTGTCAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9221|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9221|e       720 GGTACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[3]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9223|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9223|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9223|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9223|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9223|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9223|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9223|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9223|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9223|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9223|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9223|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9223|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACTGCCATC

gi|9223|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9223|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9223|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9223|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9223|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9223|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9223|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9223|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9223|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9223|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9223|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9223|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9223|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9223|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[4]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9225|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9225|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9225|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9225|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9225|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9225|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTAACC

gi|9225|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9225|e       180 TTCTACCCCTATGATGTGACAGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9225|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9225|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCTTGGACGATCAC

gi|9225|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9225|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACGGCCATC

gi|9225|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9225|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9225|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9225|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9225|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9225|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9225|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9225|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9225|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9225|e       600 GAGCCCCAGGTGGCTGAGAAGCTCCTGGCTCACCCAACCCAGCCCTCGTTGGCCTGCGCC

gi|9225|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9225|e       660 CAGAACTTTGTCAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9225|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9225|e       720 GGTACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[5]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9227|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9227|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9227|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9227|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGAGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9227|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9227|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9227|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9227|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9227|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9227|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9227|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9227|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACGGCCATC

gi|9227|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9227|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9227|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9227|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9227|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9227|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACC

gi|9227|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9227|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9227|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9227|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9227|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9227|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9227|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9227|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[6]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9229|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9229|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9229|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9229|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9229|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9229|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9229|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9229|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9229|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9229|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9229|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9229|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACTGCCATC

gi|9229|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9229|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9229|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9229|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9229|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9229|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9229|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9229|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9229|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9229|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9229|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9229|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9229|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9229|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[7]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9231|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9231|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9231|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9231|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGCGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9231|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9231|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9231|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9231|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9231|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9231|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9231|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9231|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACTGCCATC

gi|9231|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9231|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9231|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9231|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9231|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9231|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9231|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9231|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9231|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9231|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9231|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9231|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCTATCTGGAAACTGGACTTG

gi|9231|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9231|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[8]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9233|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9233|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9233|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9233|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9233|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9233|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9233|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9233|e       180 TTCTACCCATACGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9233|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9233|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9233|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9233|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACGGCCATC

gi|9233|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9233|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9233|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9233|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9233|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9233|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9233|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9233|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9233|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9233|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9233|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9233|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9233|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9233|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[9]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9235|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9235|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9235|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9235|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9235|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9235|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9235|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9235|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9235|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9235|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9235|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9235|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACTGCCATC

gi|9235|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9235|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9235|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9235|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9235|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9235|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9235|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9235|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9235|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9235|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9235|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9235|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9235|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9235|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCATTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[10]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9237|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9237|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9237|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9237|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9237|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9237|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9237|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9237|e       180 TTCTACCCCTACGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9237|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9237|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9237|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9237|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGACTGGTGAACACCACGACGGCCATC

gi|9237|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9237|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9237|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9237|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9237|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9237|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9237|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9237|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9237|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9237|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9237|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9237|e       660 CAGAACTTTGTGAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9237|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9237|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[11]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9239|e         0 M  A  F  T  L  T  N  K  N  V  V  F  V  A  G  L  G  G  I  G  
gi|9239|e         0 ATGGCGTTTACCTTGACCAACAAGAACGTGGTTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|9239|e        20 L  D  T  S  K  E  L  V  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9239|e        60 CTGGACACCAGCAAGGAGCTGGTCAAGCGGGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9239|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9239|e       120 ATTGAGAACCCGGCTGCCATCGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9239|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9239|e       180 TTCTACCCCTACGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9239|e        80 F  A  Q  L  K  T  I  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9239|e       240 TTCGCCCAGCTGAAGACCATCGATGTCCTGATCAACGGAGCTGGCATCCTGGACGATCAC

gi|9239|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9239|e       300 CAGATCGAGCGCACCATCGCCGTCAACTACACCGGCCTGGTGAACACCACGACGGCCATC

gi|9239|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9239|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGACCCGGTGGTATCATCTGCAACATTGGATCC

gi|9239|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9239|e       420 GTGACTGGATTCAACGCCATCTACCAGGTGCCCGTTTACTCCGGCACCAAGGCTGCCGTG

gi|9239|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9239|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATCACCGGCGTGACCGCTTACACC

gi|9239|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9239|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9239|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9239|e       600 GAGCCCCAGGTGGCCGAGAAGCTCCTGGCTCACCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9239|e       220 Q  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9239|e       660 CAGAACTTTGTCAAGGCCATCGAGCTGAACCAGAACGGTGCCATCTGGAAACTGGACTTG

gi|9239|e       240 G  T  L  E  A  I  Q  W  S  K  H  W  D  S  G  I   256
gi|9239|e       720 GGCACCCTGGAGGCCATCCAGTGGTCCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[12]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9097|e         0 M  A  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|9097|e         0 ATGGCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATTGGT

gi|9097|e        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9097|e        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9097|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9097|e       120 ATTGAGAACCCTGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9097|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9097|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9097|e        80 F  A  K  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9097|e       240 TTCGCCAAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|9097|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9097|e       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|9097|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9097|e       360 TTGGACTTCTGGGACAAGCGCAAGGGTGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|9097|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9097|e       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCTGCCGTG

gi|9097|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9097|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACC

gi|9097|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9097|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9097|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9097|e       600 GAGCCCCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9097|e       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9097|e       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|9097|e       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|9097|e       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[13]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9099|e         0 M  A  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|9099|e         0 ATGGCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATTGGT

gi|9099|e        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9099|e        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9099|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9099|e       120 ATTGAGAACCCTGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9099|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9099|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9099|e        80 F  A  K  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9099|e       240 TTCGCCAAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|9099|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9099|e       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|9099|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9099|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|9099|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9099|e       420 GTCACTGGTTTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCTGCCGTG

gi|9099|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9099|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACC

gi|9099|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9099|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9099|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9099|e       600 GAGCCCCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACTCAGCCCTCATTGGCCTGCGCC

gi|9099|e       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9099|e       660 GAGAACTTCGTCAAGGCCATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|9099|e       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|9099|e       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[14]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9101|e         0 M  A  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|9101|e         0 ATGGCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATTGGT

gi|9101|e        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9101|e        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9101|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9101|e       120 ATTGAGAACCCTGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9101|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9101|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9101|e        80 F  A  K  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9101|e       240 TTCGCCAAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|9101|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9101|e       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|9101|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9101|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|9101|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9101|e       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCTGGCACCAAGGCCGCCGTG

gi|9101|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9101|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACC

gi|9101|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9101|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9101|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9101|e       600 GAGCCCCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9101|e       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9101|e       660 GAGAACTTCGTCAAGGCCATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|9101|e       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|9101|e       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[15]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|9103|e         0 M  A  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|9103|e         0 ATGGCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATCGGT

gi|9103|e        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|9103|e        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGACCTGAAGAACCTGGTGATCCTCGACCGC

gi|9103|e        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|9103|e       120 ATTGAGAACCCTGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|9103|e        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|9103|e       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|9103|e        80 F  A  K  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|9103|e       240 TTCGCCAAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|9103|e       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|9103|e       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|9103|e       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|9103|e       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|9103|e       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|9103|e       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|9103|e       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|9103|e       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACC

gi|9103|e       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|9103|e       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGCTGGATGTT

gi|9103|e       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|9103|e       600 GAGCCCCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|9103|e       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|9103|e       660 GAGAACTTCGTCAAGGCCATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|9103|e       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|9103|e       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[16]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156879         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156879         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|156879        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156879        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156879        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156879       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156879        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156879       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156879        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156879       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156879       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156879       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156879       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156879       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCAGGTGGTATCATCTGCAACATTGGATCC

gi|156879       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156879       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156879       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156879       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACGGCTTACACT

gi|156879       180 V  N  P  G  I  T  R  T  T  L  V  H  T  F  N  S  W  L  D  V  
gi|156879       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACACGTTCAACTCCTGGTTGGATGTT

gi|156879       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156879       600 GAGCCTCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|156879       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156879       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156879       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156879       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[17]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156877         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156877         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|156877        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156877        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156877        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156877       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156877        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156877       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156877        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156877       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156877       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156877       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156877       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156877       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156877       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156877       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156877       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156877       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACGGCTTACACT

gi|156877       180 V  N  P  G  I  T  R  T  T  L  V  H  T  F  N  S  W  L  D  V  
gi|156877       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACACGTTCAACTCCTGGTTGGATGTT

gi|156877       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156877       600 GAGCCTCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|156877       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156877       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156877       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156877       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[18]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156875         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156875         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|156875        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156875        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156875        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156875       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156875        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156875       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156875        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156875       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156875       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156875       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156875       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156875       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156875       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156875       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156875       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156875       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACGGCTTACACT

gi|156875       180 V  N  P  G  I  T  R  T  T  L  V  H  T  F  N  S  W  L  D  V  
gi|156875       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACACGTTCAACTCCTGGTTGGATGTT

gi|156875       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156875       600 GAGCCTCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|156875       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156875       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156875       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156875       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[19]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156873         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156873         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|156873        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156873        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156873        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156873       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156873        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156873       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156873        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156873       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156873       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156873       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156873       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156873       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156873       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156873       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156873       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156873       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACGGCTTACACT

gi|156873       180 V  N  P  G  I  T  R  T  T  L  V  H  T  F  N  S  W  L  D  V  
gi|156873       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACACGTTCAACTCCTGGTTGGATGTT

gi|156873       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156873       600 GAGCCTCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|156873       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156873       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156873       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156873       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[20]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156871         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156871         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|156871        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156871        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156871        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156871       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156871        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156871       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156871        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156871       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156871       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156871       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156871       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156871       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156871       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156871       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156871       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156871       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACGGCTTACACT

gi|156871       180 V  N  P  G  I  T  R  T  T  L  V  H  T  F  N  S  W  L  D  V  
gi|156871       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACACGTTCAACTCCTGGTTGGATGTT

gi|156871       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156871       600 GAGCCTCAGGTTGCCGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|156871       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156871       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156871       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156871       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[21]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156863         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156863         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATTGGT

gi|156863        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156863        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156863        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156863       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156863        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156863       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156863        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156863       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156863       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156863       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156863       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156863       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156863       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156863       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156863       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156863       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACC

gi|156863       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|156863       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGTTGGATGTT

gi|156863       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156863       600 GAGCCCCAGGTTGCTGAGAAGCTCCTGGCTCATCCCACCCAGCCATCGTTGGCCTGCGCC

gi|156863       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156863       660 GAGAACTTCGTCAAGGCTATCGAACTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156863       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156863       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[22]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156869         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156869         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|156869        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156869        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156869        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156869       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156869        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156869       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156869        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156869       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156869       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156869       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156869       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156869       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156869       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156869       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156869       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156869       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACT

gi|156869       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|156869       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGTTGGATGTT

gi|156869       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156869       600 GAGCCTCAGGTTGCTGAGAAGCTCCTGGCTCATCCCACCCAGCCCTCGTTGGCCTGCGCC

gi|156869       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156869       660 GAGAACTTCGTCAAGGCTATCGAACTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156869       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156869       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[23]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156867         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156867         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTGGCCGGTCTGGGAGGCATTGGT

gi|156867        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156867        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156867        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156867       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156867        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156867       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156867        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156867       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156867       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156867       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156867       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156867       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156867       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156867       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156867       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156867       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACC

gi|156867       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|156867       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGTTGGATGTT

gi|156867       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156867       600 GAGCCCCAGGTTGCTGAGAAGCTCCTGGCTCATCCCACCCAGCCATCGTTGGCCTGCGCC

gi|156867       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156867       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156867       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156867       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[24]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156865         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156865         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATTGGT

gi|156865        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156865        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156865        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156865       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156865        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156865       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGCTGCTGAAGACCATC

gi|156865        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156865       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156865       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156865       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156865       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156865       360 CTGGACTTCTGGGACAAGCGCAAGGGCGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156865       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156865       420 GTCACTGGATTCAATGCCATCTACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156865       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156865       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCCCCCATTACCGGCGTGACCGCTTACACC

gi|156865       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|156865       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGTTGGATGTT

gi|156865       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156865       600 GAGCCCCAGGTTGCTGAGAAGCTCCTGGCTCATCCCACCCAGCCATCGTTGGCCTGCGCC

gi|156865       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156865       660 GAGAACTTCGTCAAGGCTATCGAACTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156865       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156865       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[25]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156861         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156861         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATTGGT

gi|156861        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156861        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156861        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156861       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156861        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156861       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGTTGCTGAAGACCATC

gi|156861        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156861       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156861       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156861       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCTATT

gi|156861       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156861       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156861       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156861       420 GTCACTGGATTCAATGCCATATACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156861       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156861       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCACCCATCACCGGCGTGACCGCTTACACC

gi|156861       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|156861       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGTTGGATGTT

gi|156861       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156861       600 GAGCCCCAGGTTGCTGAGAAGCTCCTGGCTCATCCCACCCAGCCATCGTTGGCCTGCGCC

gi|156861       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156861       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156861       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156861       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        protein_record = protein_alignment.sequences[26]
        nucleotide_record = nucleotide_records[protein_record.id]
        self.assertEqual(nucleotide_record.id, protein_record.id)
        alignments = aligner.align(protein_record, nucleotide_record)
        self.assertEqual(len(alignments), 1)
        alignment = next(alignments)
        codon_alignments.append(alignment)
        self.assertTrue(
            np.array_equal(alignment.coordinates, np.array([[0, 256], [0, 768]]))
        )
        self.assertEqual(
            str(alignment),
            """\
gi|156859         0 M  S  F  T  L  T  N  K  N  V  I  F  V  A  G  L  G  G  I  G  
gi|156859         0 ATGTCGTTTACTTTGACCAACAAGAACGTGATTTTCGTTGCCGGTCTGGGAGGCATTGGT

gi|156859        20 L  D  T  S  K  E  L  L  K  R  D  L  K  N  L  V  I  L  D  R  
gi|156859        60 CTGGACACCAGCAAGGAGCTGCTCAAGCGCGATCTGAAGAACCTGGTGATCCTCGACCGC

gi|156859        40 I  E  N  P  A  A  I  A  E  L  K  A  I  N  P  K  V  T  V  T  
gi|156859       120 ATTGAGAACCCGGCTGCCATTGCCGAGCTGAAGGCAATCAATCCAAAGGTGACCGTCACC

gi|156859        60 F  Y  P  Y  D  V  T  V  P  I  A  E  T  T  K  L  L  K  T  I  
gi|156859       180 TTCTACCCCTATGATGTGACCGTGCCCATTGCCGAGACCACCAAGTTGCTGAAGACCATC

gi|156859        80 F  A  Q  L  K  T  V  D  V  L  I  N  G  A  G  I  L  D  D  H  
gi|156859       240 TTCGCCCAGCTGAAGACCGTCGATGTCCTGATCAACGGAGCTGGTATCCTGGACGATCAC

gi|156859       100 Q  I  E  R  T  I  A  V  N  Y  T  G  L  V  N  T  T  T  A  I  
gi|156859       300 CAGATCGAGCGCACCATTGCCGTCAACTACACTGGCCTGGTCAACACCACGACGGCCATT

gi|156859       120 L  D  F  W  D  K  R  K  G  G  P  G  G  I  I  C  N  I  G  S  
gi|156859       360 CTGGACTTCTGGGACAAGCGCAAGGGTGGTCCCGGTGGTATCATCTGCAACATTGGATCC

gi|156859       140 V  T  G  F  N  A  I  Y  Q  V  P  V  Y  S  G  T  K  A  A  V  
gi|156859       420 GTCACTGGATTCAATGCCATATACCAGGTGCCCGTCTACTCCGGCACCAAGGCCGCCGTG

gi|156859       160 V  N  F  T  S  S  L  A  K  L  A  P  I  T  G  V  T  A  Y  T  
gi|156859       480 GTCAACTTCACCAGCTCCCTGGCGAAACTGGCACCCATCACCGGCGTGACCGCTTACACC

gi|156859       180 V  N  P  G  I  T  R  T  T  L  V  H  K  F  N  S  W  L  D  V  
gi|156859       540 GTGAACCCCGGCATCACCCGCACCACCCTGGTGCACAAGTTCAACTCCTGGTTGGATGTT

gi|156859       200 E  P  Q  V  A  E  K  L  L  A  H  P  T  Q  P  S  L  A  C  A  
gi|156859       600 GAGCCCCAGGTTGCTGAGAAGCTCCTGGCTCATCCCACCCAGCCATCGTTGGCCTGCGCC

gi|156859       220 E  N  F  V  K  A  I  E  L  N  Q  N  G  A  I  W  K  L  D  L  
gi|156859       660 GAGAACTTCGTCAAGGCTATCGAGCTGAACCAGAACGGAGCCATCTGGAAACTGGACTTG

gi|156859       240 G  T  L  E  A  I  Q  W  T  K  H  W  D  S  G  I   256
gi|156859       720 GGCACCCTGGAGGCCATCCAGTGGACCAAGCACTGGGACTCCGGCATC 768
""",
        )
        nucleotide_records.close()  # Close indexed FASTA file
        alignment = protein_alignment.mapall(codon_alignments)
        unique_species = [
            "Drosophila simulans",
            "Drosophila yakuba",
            "D.melanogaster",
        ]
        species = []
        for record in alignment.sequences:
            description = record.description
            for s in unique_species:
                if s in description:
                    break
            else:
                raise Exception(f"Failed to find species for {description}")
            species.append(s)
        pvalue = mktest(alignment, species)
        self.assertAlmostEqual(pvalue, 0.0020645725725430097)


if __name__ == "__main__":
    runner = unittest.TextTestRunner(verbosity=2)
    unittest.main(testRunner=runner)