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import warnings
from textwrap import dedent
from . import expected
from gffutils import example_filename, create, feature
import gffutils
import gffutils.helpers as helpers
import gffutils.gffwriter as gffwriter
import gffutils.inspect as inspect
import gffutils.iterators as iterators
import sys
import os
import shutil
import threading
import tempfile
import http.server as SimpleHTTPServer
if sys.version_info.major == 3:
import socketserver as SocketServer
else:
import SocketServer
import multiprocessing
import json
import difflib
import pytest
testdbfn_gtf = ":memory:"
testdbfn_gff = ":memory:"
fn = gffutils.example_filename("FBgn0031208.gtf")
def make_db(i):
"""
Module-level function that can be pickled across processes for
multiprocessing testing.
"""
gffutils.create_db(fn, ":memory:", _keep_tempfiles=".%s" % i)
return i
def test_update():
# check both in-memory and file-based dbs
db = create.create_db(
example_filename("FBgn0031208.gff"),
":memory:",
verbose=False,
keep_order=True,
force=True,
)
orig_num_features = len(list(db.all_features()))
f = feature.feature_from_line(
"chr2L . testing 1 10 . + . ID=testing_feature;n=1",
dialect=db.dialect,
strict=False,
)
# no merge strategy required because we're adding a new feature
db.update([f])
x = list(db.features_of_type("testing"))
assert len(x) == 1
x = x[0]
x.keep_order = True
assert str(x) == "chr2L . testing 1 10 . + . ID=testing_feature;n=1", str(x)
# ought to be one more now . . .
num_features = len(list(db.all_features()))
assert num_features == orig_num_features + 1, num_features
# Now try updating with the same feature, but using merge_strategy="merge",
# which appends items to attributes ( n=1 --> n=1,2 )
f = feature.feature_from_line(
"chr2L . testing 1 10 . + . ID=testing_feature;n=1",
dialect=db.dialect,
strict=False,
)
f.keep_order = True
f.attributes["n"] = ["2"]
db.update([f], merge_strategy="merge")
x = list(db.features_of_type("testing"))
assert len(x) == 1
# Merging does a list(set()) operation, so the order is not guaranteed.
# Fix it here for testing...
x = x[0]
x.attributes["n"].sort()
assert str(x) == "chr2L . testing 1 10 . + . ID=testing_feature;n=1,2", str(x)
# still should have the same number of features as before (still 2)
num_features = len(list(db.all_features()))
assert num_features == orig_num_features + 1, num_features
import logging
logging.basicConfig(level=logging.DEBUG)
# Merging while iterating. e.g., if you're updating children with gene
# IDs.
db = create.create_db(
example_filename("FBgn0031208.gff"),
"tmp.db",
verbose=False,
force=True,
keep_order=True,
)
def gen():
for gene in db.features_of_type("gene"):
for child in list(db.children(gene)):
# important: the FBgn0031208.gff file was designed to have some
# funky features: there are two exons without ID attributes. These
# are assigned to ids "exon_1" and "exon_2". Upon update, with
# still no ID, we then have two new features "exon_3" and "exon_4".
# To prevent this issue, we ensure that the ID attribute exists...
child.attributes["gene_id"] = [gene.id]
if "ID" not in child.attributes:
child.attributes["ID"] = [child.id]
if child.featuretype == "five_prime_UTR":
print(child)
yield child
db = db.update(gen(), merge_strategy="replace")
print("----------------------------------")
for child in db.children("FBgn0031208"):
if child.featuretype == "five_prime_UTR":
print(child)
assert child.attributes["gene_id"] == ["FBgn0031208"], (child, child.attributes)
num_entries = 0
for gene_recs in list(db.iter_by_parent_childs()):
# Add attribute to each gene record
rec = gene_recs[0]
rec.attributes["new"] = ["new_value"]
db.update([rec], merge_strategy="replace")
num_entries += 1
print(list(db.all_features()))
assert num_entries > 1, "Only %d left after update" % (num_entries)
# Replace
f = feature.feature_from_line(
"chr2L . testing 1 10 . + . ID=testing_feature;n=1",
dialect=db.dialect,
strict=False,
)
f.keep_order = True
f.attributes["n"] = ["3"]
db.update([f], merge_strategy="replace")
x = list(db.features_of_type("testing"))
assert len(x) == 1
assert str(x[0]) == "chr2L . testing 1 10 . + . ID=testing_feature;n=3", str(x[0])
# still should have the same number of features as before (still 2)
num_features = len(list(db.all_features()))
assert num_features == orig_num_features + 1, num_features
# Same thing, but GTF instead of GFF.
db = create.create_db(
example_filename("FBgn0031208.gtf"),
":memory:",
verbose=False,
force=True,
keep_order=True,
)
f = feature.feature_from_line(
'chr2L . testing 1 10 . + . gene_id "fake"; n "1"', strict=False
)
f.keep_order = True
db.update([f], merge_strategy="merge")
x = list(db.features_of_type("testing"))
assert len(x) == 1
x = x[0]
x.keep_order = True
# note the trailing semicolon. That's because the db's dialect has
# ['trailing semicolon'] = True.
assert str(x) == 'chr2L . testing 1 10 . + . gene_id "fake"; n "1";', str(x)
class BaseDB(object):
"""
Generic test class. Run different versions by subclassing and overriding orig_fn.
"""
orig_fn = None
def setup_method(self):
def gff_id_func(f):
if "ID" in f.attributes:
return f.attributes["ID"][0]
elif "Name" in f.attributes:
return f.attributes["Name"][0]
else:
return "{0.featuretype}:{0.seqid}:{0.start}-{0.end}:{0.strand}".format(
f
)
def gtf_id_func(f):
if f.featuretype == "gene":
if "gene_id" in f.attributes:
return f.attributes["gene_id"][0]
elif f.featuretype == "transcript":
if "transcript_id" in f.attributes:
return f.attributes["transcript_id"][0]
else:
return "{0.featuretype}:{0.seqid}:{0.start}-{0.end}:{0.strand}".format(
f
)
if self.orig_fn.endswith(".gtf"):
id_func = gtf_id_func
if self.orig_fn.endswith(".gff"):
id_func = gff_id_func
self.db = create.create_db(
self.orig_fn,
":memory:",
id_spec=id_func,
merge_strategy="create_unique",
verbose=False,
keep_order=True,
)
self.c = self.db.conn.cursor()
self.dialect = self.db.dialect
def table_test(self):
expected_tables = [
"features",
"relations",
"meta",
"directives",
"autoincrements",
"duplicates",
"sqlite_stat1",
]
self.c.execute('select name from sqlite_master where type="table"')
observed_tables = [
i[0]
for i in self.c.execute('select name from sqlite_master where type="table"')
]
assert set(expected_tables) == set(observed_tables), observed_tables
def _count1(self, featuretype):
"""Count using SQL"""
self.c.execute(
"select count() from features where featuretype = ?", (featuretype,)
)
results = self.c.fetchone()[0]
print('count1("%s") says: %s' % (featuretype, results))
return results
def _count2(self, featuretype):
"""Count GFF lines"""
cnt = 0
for line in open(self.orig_fn):
if line.startswith("#"):
continue
L = line.split()
if len(L) < 3:
continue
if L[2] == featuretype:
cnt += 1
print('count2("%s") says: %s' % (featuretype, cnt))
return cnt
def _count3(self, featuretype):
"""Count with the count_features_of_type method"""
results = self.db.count_features_of_type(featuretype)
print('count3("%s") says: %s' % (featuretype, results))
return results
def _count4(self, featuretype):
"""Count by iterating over all features of this type"""
cnt = 0
for i in self.db.features_of_type(featuretype):
cnt += 1
print('count4("%s") says: %s' % (featuretype, cnt))
return cnt
def featurecount_test(self):
# Right number of each featuretype, using multiple different ways of
# counting?
print("format:", self.dialect["fmt"])
expected_feature_counts = expected.expected_feature_counts[self.dialect["fmt"]]
for featuretype, expected_count in expected_feature_counts.items():
rawsql_cnt = self._count1(featuretype)
fileparsed_cnt = self._count2(featuretype)
count_feature_of_type_cnt = self._count3(featuretype)
iterator_cnt = self._count4(featuretype)
print("expected count:", expected_count)
assert (
rawsql_cnt
== count_feature_of_type_cnt
== iterator_cnt
== fileparsed_cnt
== expected_count
)
def _expected_parents(self):
if self.dialect["fmt"] == "gff3":
parents1 = expected.GFF_parent_check_level_1
parents2 = expected.GFF_parent_check_level_2
if self.dialect["fmt"] == "gtf":
parents1 = expected.GTF_parent_check_level_1
parents2 = expected.GTF_parent_check_level_2
return parents1, parents2
def test_parents_level_1(self):
parents1, parents2 = self._expected_parents()
for child, expected_parents in parents1.items():
observed_parents = [i.id for i in self.db.parents(child, level=1)]
print("observed parents for %s:" % child, set(observed_parents))
print("expected parents for %s:" % child, set(expected_parents))
assert set(observed_parents) == set(expected_parents)
def test_parents_level_2(self):
parents1, parents2 = self._expected_parents()
for child, expected_parents in parents2.items():
observed_parents = [i.id for i in self.db.parents(child, level=2)]
print(self.db[child])
print("observed parents for %s:" % child, set(observed_parents))
print("expected parents for %s:" % child, set(expected_parents))
assert set(observed_parents) == set(expected_parents)
def test_bed12(self):
if self.__class__ == TestGFFClass:
kwargs = dict(
block_featuretype="exon", thick_featuretype="CDS", name_field="ID"
)
if self.__class__ == TestGTFClass:
kwargs = dict(
block_featuretype="exon",
thick_featuretype="CDS",
name_field="transcript_id",
)
obs = self.db.bed12("FBtr0300689", **kwargs)
exp = "chr2L 7528 9484 FBtr0300689 0 + 7679 8610 0,0,0 2 588,1292 0,664"
assert obs == exp
obs = self.db.bed12("FBtr0300690", **kwargs)
exp = "chr2L 7528 9484 FBtr0300690 0 + 7679 9276 0,0,0 3 588,397,817 0,664,1139"
assert obs == exp
class TestGFFClass(BaseDB):
orig_fn = example_filename("FBgn0031208.gff")
class TestGTFClass(BaseDB):
orig_fn = example_filename("FBgn0031208.gtf")
def test_random_chr():
"""
Test on GFF files with random chromosome events.
"""
gff_fname = gffutils.example_filename("random-chr.gff")
db = helpers.get_gff_db(gff_fname)
# Test that we can get children of only a selected type
gene_id = "chr1_random:165882:165969:-@chr1_random:137473:137600:-@chr1_random:97006:97527:-"
mRNAs = db.children(gene_id, featuretype="mRNA")
for mRNA_entry in mRNAs:
assert (
mRNA_entry.featuretype == "mRNA"
), "Not all entries are of type mRNA! %s" % (
",".join([entry.featuretype for entry in mRNAs])
)
print("Parsed random chromosome successfully.")
def test_gffwriter():
"""
Test GFFWriter.
"""
print("Testing GFF writer..")
fn = gffutils.example_filename("unsanitized.gff")
# Make a copy of it as temporary named file
temp_f = tempfile.NamedTemporaryFile(delete=False)
temp_fname_source = temp_f.name
shutil.copy(fn, temp_fname_source)
# Now write file in place
source_first_line = open(temp_fname_source, "r").readline().strip()
assert not source_first_line.startswith(
"#GFF3"
), "unsanitized.gff should not have a gffutils-style header."
db_in = gffutils.create_db(fn, ":memory:", keep_order=True)
# Fetch first record
rec = next(db_in.all_features())
##
## Write GFF file in-place test
##
print("Testing in-place writing")
gff_out = gffwriter.GFFWriter(temp_fname_source, in_place=True, with_header=True)
gff_out.write_rec(rec)
gff_out.close()
# Ensure that the file was written with header
rewritten = open(temp_fname_source, "r")
new_header = rewritten.readline().strip()
assert new_header.startswith(
"#GFF3"
), "GFFWriter serialized files should have a #GFF3 header."
print(" - Wrote GFF file in-place successfully.")
##
## Write GFF file to new file test
##
print("Testing writing to new file")
new_file = tempfile.NamedTemporaryFile(delete=False)
gff_out = gffwriter.GFFWriter(new_file.name)
gff_out.write_rec(rec)
gff_out.close()
new_line = open(new_file.name, "r").readline().strip()
assert new_line.startswith("#GFF3"), "GFFWriter could not write to a new GFF file."
print(" - Wrote to new file successfully.")
# def test_attributes_modify():
# """
# Test that attributes can be modified in a GFF record.
# TODO: This test case fails?
# """
# # Test that attributes can be modified
# db = gffutils.create_db(gffutils.example_filename('FBgn0031208.gff'), testdbfn_gff,
# verbose=False,
# force=True)
# gene_id = "FBgn0031208"
# gene_childs = list(db.children(gene_id))
# print("First child is not an mRNA")
# print(gene_childs[0].featuretype)
# assert str(gene_childs[0].attributes) == 'ID=FBtr0300689;Name=CG11023-RB;Parent=FBgn0031208;Dbxref=FlyBase_Annotation_IDs:CG11023-RB;score_text=Strongly Supported;score=11'
# gene_childs[0].attributes["ID"] = "Modified"
# assert str(gene_childs[0].attributes) == 'ID=Modified;Name=CG11023-RB;Parent=FBgn0031208;Dbxref=FlyBase_Annotation_IDs:CG11023-RB;score_text=Strongly Supported;score=11;ID=Modified'
# ###
# ### NOTE: Would be ideal if database checked that this
# ### change leaves "dangling" children; i.e. children
# ### GFF nodes that point to Parent that does not exist.
# ###
def test_create_db_from_iter():
"""
Test creation of FeatureDB from iterator.
"""
print("Testing creation of DB from iterator")
db_fname = gffutils.example_filename("gff_example1.gff3")
db = gffutils.create_db(db_fname, ":memory:", keep_order=True)
def my_iterator():
for rec in db.all_features():
yield rec
new_db = gffutils.create_db(my_iterator(), ":memory:", keep_order=True)
print(list(new_db.all_features()))
gene_feats = new_db.all_features(featuretype="gene")
assert len(list(gene_feats)) != 0, "Could not load genes from GFF."
def test_sanitize_gff():
"""
Test sanitization of GFF. Should be merged with GFF cleaning
I believe unless they are intended to have different functionalities.
"""
# Get unsanitized GFF
fn = gffutils.example_filename("unsanitized.gff")
# Get its database
db = helpers.get_gff_db(fn)
# Sanitize the GFF
sanitized_recs = helpers.sanitize_gff_db(db)
# Ensure that sanitization work, meaning all
# starts must be less than or equal to stops
for rec in sanitized_recs.all_features():
assert rec.start <= rec.stop, "Sanitization failed."
print("Sanitized GFF successfully.")
def test_region():
db_fname = gffutils.example_filename("FBgn0031208.gff")
db = gffutils.create_db(db_fname, ":memory:", keep_order=True)
def _check(item):
kwargs, expected = item
try:
obs = list(db.region(**kwargs))
assert len(obs) == expected, "expected %s got %s" % (expected, len(obs))
except expected:
pass
regions = [
# previously failed, see issue #45
(dict(seqid="chr2L", start=1, end=2e9, completely_within=True), 27),
(dict(region="chr2L", start=0), ValueError),
(dict(region="chr2L", end=0), ValueError),
(dict(region="chr2L", seqid=0), ValueError),
# these coords should catch everything
(dict(region="chr2L:7529-12500"), 27),
# stranded versions:
(dict(region="chr2L:7529-12500", strand="."), 0),
(dict(region="chr2L:7529-12500", strand="+"), 21),
(dict(region="chr2L:7529-12500", strand="-"), 6),
# different ways of selecting only that last exon in the last gene:
(dict(seqid="chr2L", start=11500, featuretype="exon"), 1),
(dict(seqid="chr2L", start=9500, featuretype="exon", strand="+"), 1),
# alternative method
(dict(seqid="chr2L", start=7529, end=12500), 27),
# since default completely_within=False, this catches anything that
# falls after 7680. So it only excludes the 5'UTR, which ends at 7679.
(dict(seqid="chr2L", start=7680), 26),
# but completely_within=True will exclude the gene and mRNAs, first
# exon and the 5'UTR
(dict(seqid="chr2L", start=7680, completely_within=True), 22),
# similarly, this will *exclude* anything before 7680
(dict(seqid="chr2L", end=7680), 5),
# and also similarly, this will only get us the 5'UTR which is the only
# feature falling completely before 7680
(dict(seqid="chr2L", end=7680, completely_within=True), 1),
# and there's only features from chr2L in this file, so this catches
# everything too
(dict(region="chr2L"), 27),
# using seqid should work similarly to `region` with only chromosome
(dict(seqid="chr2L"), 27),
# nonexistent
(dict(region="nowhere"), 0),
]
for item in regions:
_check(item)
def test_nonascii():
# smoke test (prev. version returned Unicode)
#
db = gffutils.create_db(
gffutils.example_filename("nonascii"), ":memory:", keep_order=True
)
for i in db.all_features():
# this works in IPython, or using nosetests --with-doctest...
try:
print(i)
# ...but fails using plain nosetests or when using regular Python
# interpreter
except UnicodeEncodeError:
print(str(i))
def test_feature_merge():
# both "n" attribute and "source" field should be merged, since
# force_merge_fields=['source'].
gtfdata = dedent(
"""
chr1 a testing 1 10 . + . gene_id "fake"; n "2";
chr1 b testing 1 10 . + . gene_id "fake"; n "1";
"""
)
db = gffutils.create_db(
gtfdata,
":memory:",
from_string=True,
merge_strategy="merge",
id_spec="gene_id",
force_merge_fields=["source"],
keep_order=True,
sort_attribute_values=True,
)
assert db.dialect["fmt"] == "gtf"
assert len(list(db.all_features())) == 1
x = db["fake"]
x.keep_order = True
x.attributes["n"].sort()
assert str(x) == 'chr1 a,b testing 1 10 . + . gene_id "fake"; n "1,2";', str(x)
gffdata = dedent(
"""
chr1 a testing 1 10 . + . gene_id="fake"; n="2";
chr1 b testing 1 10 . + . gene_id="fake"; n="1";
"""
)
db = gffutils.create_db(
gffdata,
":memory:",
from_string=True,
merge_strategy="merge",
id_spec="gene_id",
force_merge_fields=["source"],
keep_order=True,
)
assert db.dialect["fmt"] == "gff3"
assert len(list(db.all_features())) == 1
x = db["fake"]
x.attributes["n"].sort()
x.keep_order = True
assert str(x) == 'chr1 a,b testing 1 10 . + . gene_id="fake"; n="1,2";', str(x)
# But when not using force_merge_fields, there should be separate entries;
# accessing fake and fake_1 should not give FeatureNotFound errors.
db = gffutils.create_db(
gtfdata,
":memory:",
from_string=True,
merge_strategy="merge",
id_spec="gene_id",
keep_order=True,
)
assert len(list(db.all_features())) == 2
x = db["fake"]
y = db["fake_1"]
db = gffutils.create_db(
gffdata,
":memory:",
from_string=True,
merge_strategy="merge",
id_spec="gene_id",
keep_order=True,
)
assert len(list(db.all_features())) == 2
x = db["fake"]
y = db["fake_1"]
with pytest.raises(ValueError):
gffutils.create_db(
gtfdata,
":memory:",
from_string=True,
merge_strategy="merge",
id_spec="gene_id",
force_merge_fields=["start"],
keep_order=True,
)
# test that warnings are raised because of strand and frame
with warnings.catch_warnings(record=True) as w:
gffdata = dedent(
"""
chr1 a testing 1 10 . + . gene_id="fake"; n="2";
chr1 a testing 1 10 . - 1 gene_id="fake"; n="1";
"""
)
db = gffutils.create_db(
gffdata,
":memory:",
from_string=True,
merge_strategy="merge",
id_spec="gene_id",
force_merge_fields=["strand", "frame"],
keep_order=True,
sort_attribute_values=True,
)
assert db.dialect["fmt"] == "gff3"
assert len(list(db.all_features())) == 1
x = db["fake"]
x.keep_order = True
x.attributes["n"].sort()
assert str(x) == 'chr1 a testing 1 10 . +,- .,1 gene_id="fake"; n="1,2";', str(
x
)
assert len(w) == 2
def test_add_relation():
db = gffutils.create_db(
gffutils.example_filename("FBgn0031208.gff"), ":memory:", keep_order=True
)
L = len(list(db.children("FBgn0031208:3")))
assert L == 0, L
def func(parent, child):
child["Parent"] = child["Parent"] + [parent.id]
child["exon_parent"] = [parent.id]
return child
db.add_relation("FBgn0031208:3", "CDS_FBgn0031208:1_737", 1, child_func=func)
L = len(list(db.children("FBgn0031208:3")))
assert L == 1, L
L = list(db.children("FBgn0031208:3"))
x = L[0]
assert "FBgn0031208:3" in x["Parent"]
assert x["exon_parent"] == ["FBgn0031208:3"]
def test_create_db_from_url():
"""
Test creation of FeatureDB from URL iterator.
"""
print("Testing creation of DB from URL iterator")
# initially run SimpleHTTPServer at port 0 and os will take first available
Handler = SimpleHTTPServer.SimpleHTTPRequestHandler
httpd = SocketServer.TCPServer(("", 0), Handler)
port = str(httpd.socket.getsockname()[1])
print("serving at port", port)
# Serving test/data folder
served_folder = gffutils.example_filename("")
savedir = os.getcwd()
os.chdir(served_folder)
print("Starting SimpleHTTPServer in thread")
server_thread = threading.Thread(target=httpd.serve_forever)
server_thread.deamon = True
server_thread.start()
try:
url = "".join(["http://localhost:", port, "/gff_example1.gff3"])
db = gffutils.create_db(url, ":memory:", keep_order=True)
def my_iterator():
for rec in db.all_features():
yield rec
new_db = gffutils.create_db(my_iterator(), ":memory:", keep_order=True)
print(list(new_db.all_features()))
gene_feats = new_db.all_features(featuretype="gene")
assert len(list(gene_feats)) != 0, "Could not load genes from GFF."
url = "".join(["http://localhost:", port, "/gff_example1.gff3.gz"])
db = gffutils.create_db(url, ":memory:", keep_order=True)
def my_iterator():
for rec in db.all_features():
yield rec
new_db = gffutils.create_db(my_iterator(), ":memory:", keep_order=True)
print(list(new_db.all_features()))
gene_feats = new_db.all_features(featuretype="gene")
assert len(list(gene_feats)) != 0, "Could not load genes from GFF."
finally:
print("Server shutdown.")
httpd.shutdown()
server_thread.join()
os.chdir(savedir)
def test_empty_files():
fn = tempfile.NamedTemporaryFile(delete=False).name
a = open(fn, "w")
a.close()
with pytest.raises(gffutils.exceptions.EmptyInputError):
gffutils.create_db(fn, fn + ".db")
def test_false_function():
# smoke test: before commit ce4b7671f, this would raise "TypeError: object
# of type 'function' has no len()"
db = gffutils.create_db(
gffutils.example_filename("FBgn0031208.gff"),
":memory:",
keep_order=True,
id_spec=lambda x: False,
merge_strategy="create_unique",
)
def test_inspect():
file_results = inspect.inspect(
gffutils.example_filename("FBgn0031208.gff"), verbose=False
)
db_results = inspect.inspect(
gffutils.create_db(gffutils.example_filename("FBgn0031208.gff"), ":memory:"),
verbose=False,
)
expected = {
"featuretype": {
"intron": 3,
"five_prime_UTR": 1,
"exon": 6,
"mRNA": 4,
"CDS": 5,
"pcr_product": 1,
"three_prime_UTR": 2,
"protein": 2,
"gene": 3,
},
"feature_count": 27,
"chrom": {
"chr2L": 27,
},
"attribute_keys": {
"": 3,
"Dbxref": 6,
"Name": 19,
"Parent": 20,
" Parent": 1,
"score_text": 2,
"gbunit": 1,
"derived_computed_cyto": 1,
"Derives_from": 2,
"derived_molecular_weight": 2,
"score": 2,
"ID": 25,
"derived_isoelectric_point": 2,
"Ontology_term": 1,
},
}
assert file_results == db_results == expected
# file and db work because db is created from
kwargs = dict(
look_for=["chrom", "strand", "attribute_keys", "featuretype"],
verbose=False,
limit=10,
)
file_results = inspect.inspect(
gffutils.example_filename("FBgn0031208.gff"), **kwargs
)
iter_results = inspect.inspect(
iter(iterators._FileIterator(gffutils.example_filename("FBgn0031208.gff"))),
**kwargs
)
db_results = inspect.inspect(
gffutils.create_db(gffutils.example_filename("FBgn0031208.gff"), ":memory:"),
**kwargs
)
expected = {
"attribute_keys": {
"Name": 9,
"Parent": 9,
"score_text": 2,
"gbunit": 1,
"derived_computed_cyto": 1,
"score": 2,
"Dbxref": 3,
"ID": 8,
"Ontology_term": 1,
},
"feature_count": 10,
"chrom": {"chr2L": 10},
"strand": {"+": 10},
"featuretype": {
"five_prime_UTR": 1,
"exon": 3,
"mRNA": 2,
"CDS": 1,
"intron": 2,
"gene": 1,
},
}
assert file_results == db_results == iter_results == expected
def test_delete():
db_fname = gffutils.example_filename("gff_example1.gff3")
# incrementally delete all features
db = gffutils.create_db(db_fname, ":memory:")
ids = [i.id for i in db.all_features()]
current = set(ids)
for _id in ids:
db.delete(_id)
expected = current.difference([_id])
current = set([i.id for i in db.all_features()])
assert current == expected, (current, expected)
assert len(current) == 0
# same thing, but as a list of Feature objects rather than string IDs
db = gffutils.create_db(db_fname, ":memory:")
features = list(db.all_features())
current = set(features)
for feature in features:
db.delete(feature)
expected = current.difference([feature])
current = set(list(db.all_features()))
assert current == expected, (current, expected)
assert len(current) == 0, current
# same thing, but use a FeatureDB.
db1 = gffutils.create_db(db_fname, ":memory:")
db2 = gffutils.create_db(db_fname, ":memory:")
db1.delete(db2)
assert len(list(db1.all_features())) == 0
db = gffutils.create_db(db_fname, ":memory:")
db.delete("nonexistent")
def test_iterator_update():
db_fname = gffutils.example_filename("gff_example1.gff3")
db = gffutils.create_db(db_fname, "tmp.db", force=True)
assert len(list(db.all_features())) == 12
orig_exon_coords = set([(i.start, i.stop) for i in db.features_of_type("exon")])
# reset all features to have the same coords of start=1, stop=100
def gen():
for i in db.features_of_type("gene"):
i.start = 1
i.stop = 100
yield i
db.update(gen(), merge_strategy="replace")
assert len(list(db.all_features())) == 12
assert len(list(db.features_of_type("gene"))) == 1
g = next(db.features_of_type("gene"))
assert g.start == 1, g.start
assert g.stop == 100, g.stop
# exons should have remained unchanged.
assert orig_exon_coords == set(
[(i.start, i.stop) for i in db.features_of_type("exon")]
)
def _transform(f):
f.start = 1
f.stop = 100
return f
db_fname = gffutils.example_filename("gff_example1.gff3")
db = gffutils.create_db(db_fname, "tmp.db", force=True)
db.update(
db.features_of_type("gene"), merge_strategy="replace", transform=_transform
)
assert len(list(db.all_features())) == 12
assert len(list(db.features_of_type("gene"))) == 1
g = next(db.features_of_type("gene"))
print(g)
assert g.start == 1, g.start
assert g.stop == 100, g.stop
# exons should have remained unchanged.
assert orig_exon_coords == set(
[(i.start, i.stop) for i in db.features_of_type("exon")]
)
def clean_tempdir():
tempfile.tempdir = tempdir
if os.path.exists(tempdir):
shutil.rmtree(tempdir)
os.makedirs(tempdir)
# specify a writeable temp dir for testing
tempdir = "/tmp/gffutils-test"
def test_tempfiles():
clean_tempdir()
# default keep_tempfiles=False should give us nothing.
db = gffutils.create_db(gffutils.example_filename("FBgn0031208.gtf"), ":memory:")
assert len(os.listdir(tempdir)) == 0
# adding keep_tempfiles=True should give us 1 tempfile for gtf...
db = gffutils.create_db(
gffutils.example_filename("FBgn0031208.gtf"), ":memory:", _keep_tempfiles=True
)
filelist = os.listdir(tempdir)
assert len(filelist) == 1, filelist
assert filelist[0].endswith(".gffutils")
# ...and another one for gff. This time, make sure the suffix
db = gffutils.create_db(
gffutils.example_filename("FBgn0031208.gff"), ":memory:", _keep_tempfiles=True
)
filelist = os.listdir(tempdir)
assert len(filelist) == 2, filelist
for i in filelist:
assert i.endswith(".gffutils")
# OK, now delete what we have so far...
clean_tempdir()
# Make sure that works for custom suffixes
db = gffutils.create_db(
gffutils.example_filename("FBgn0031208.gtf"),
":memory:",
_keep_tempfiles=".GTFtmp",
)
filelist = os.listdir(tempdir)
assert len(filelist) == 1, filelist
assert filelist[0].endswith(".GTFtmp")
clean_tempdir()
db = gffutils.create_db(
gffutils.example_filename("FBgn0031208.gtf"),
":memory:",
_keep_tempfiles=".GFFtmp",
)
filelist = os.listdir(tempdir)
assert len(filelist) == 1, filelist
assert filelist[0].endswith(".GFFtmp")
@pytest.mark.skip(reason="Unclear if still needed; currently failing")
def test_parallel_db():
# DISABLING in v0.12
# Test n parallel instances of gffutils across PROCESSES processes.
#
# Note that travis-ci doesn't like it when you use multiple cores, so the
# .travis.yml file sets this to 1. This also means that
# 1) `n` shouldn't be too large because travis-ci will run one at a time,
# but more importantly,
# 2) this will only truly test parallel processes on a local machine with
# multiple cpus.
clean_tempdir()
# .travis.yml sets the PROCESSES env var; otherwise use all available.
PROCESSES = int(os.environ.get("PROCESSES", multiprocessing.cpu_count()))
pool = multiprocessing.Pool(PROCESSES)
try:
n = 100
res = pool.map(make_db, range(n))
finally:
pool.close()
assert sorted(list(res)) == list(range(n))
filelist = os.listdir(tempdir)
assert len(filelist) == n, len(filelist)
expected = dedent(
"""\
FBtr0300689 chr2L 7529 9484 + transcript 4681 {"transcript_id":["FBtr0300689"],"gene_id":["FBgn0031208"]}
FBgn0031208 chr2L 7529 9484 + gene 4681 {"gene_id":["FBgn0031208"]}
FBtr0300690 chr2L 7529 9484 + transcript 4681 {"transcript_id":["FBtr0300690"],"gene_id":["FBgn0031208"]}
transcript_Fk_gene_1 chr2L 10000 11000 - transcript 4681 {"transcript_id":["transcript_Fk_gene_1"],"gene_id":["Fk_gene_1"]}
Fk_gene_1 chr2L 10000 11000 - gene 4681 {"gene_id":["Fk_gene_1"]}
transcript_Fk_gene_2 chr2L 11500 12500 - transcript 4681 {"transcript_id":["transcript_Fk_gene_2"],"gene_id":["Fk_gene_2"]}
Fk_gene_2 chr2L 11500 12500 - gene 4681 {"gene_id":["Fk_gene_2"]}
"""
)
def matches_expected(fn):
"""
Python 3 has unpredictable dictionary ordering. This function checks
the *semantic* similarity of lines by parsing the attributes into
a dictonary.
"""
exp_features = expected.splitlines(True)
new_features = list(open(fn))
assert len(exp_features) == len(new_features)
for expline, newline in zip(exp_features, new_features):
exp_toks = expline.split()
new_toks = newline.split()
assert exp_toks[:-1] == new_toks[:-1]
assert json.loads(exp_toks[-1]) == json.loads(new_toks[-1])
# make sure that each of the `n` files matches the expected output.
for fn in filelist:
fn = os.path.join(tempdir, fn)
try:
matches_expected(fn)
except AssertionError:
print(
"".join(difflib.ndiff(expected.splitlines(True), this.splitlines(True)))
)
raise
clean_tempdir()
def test_disable_infer():
"""
tests the new semantics for disabling gene/transcript inference
"""
# To start, we construct a GTF db by inferring genes and transcripts
db = gffutils.create_db(gffutils.example_filename("FBgn0031208.gtf"), ":memory:")
# Then create a file missing transcripts, and another missing genes.
import tempfile
tempfile.tempdir = None
no_transcripts = open(tempfile.NamedTemporaryFile(delete=False).name, "w")
no_genes = open(tempfile.NamedTemporaryFile(delete=False).name, "w")
for feature in db.all_features():
if feature.featuretype != "transcript":
no_transcripts.write(str(feature) + "\n")
if feature.featuretype != "gene":
no_genes.write(str(feature) + "\n")
no_genes.close()
no_transcripts.close()
no_tx_db = gffutils.create_db(
no_transcripts.name, ":memory:", disable_infer_transcripts=True
)
no_gn_db = gffutils.create_db(no_genes.name, ":memory:", disable_infer_genes=True)
no_xx_db = gffutils.create_db(
gffutils.example_filename("FBgn0031208.gtf"),
":memory:",
disable_infer_genes=True,
disable_infer_transcripts=True,
)
# no transcripts but 3 genes
assert len(list(no_tx_db.features_of_type("transcript"))) == 0
assert len(list(no_tx_db.features_of_type("gene"))) == 3
# no genes but 4 transcripts
assert len(list(no_gn_db.features_of_type("gene"))) == 0
assert len(list(no_gn_db.features_of_type("transcript"))) == 4
# no genes or transcripts
assert len(list(no_xx_db.features_of_type("gene"))) == 0
assert len(list(no_xx_db.features_of_type("transcript"))) == 0
def test_deprecation_handler():
return
# TODO: when infer_gene_extent actually gets deprecated, test here.
with pytest.raises(ValueError):
gffutils.create_db(
gffutils.example_filename("FBgn0031208.gtf"),
":memory:",
infer_gene_extent=False,
)
def test_nonsense_kwarg():
with pytest.raises(TypeError):
gffutils.create_db(
gffutils.example_filename("FBgn0031208.gtf"),
":memory:",
asdf=True,
)
def test_infer_gene_extent():
# Before we deprecate this, make sure it still works but emits a warning.
with warnings.catch_warnings(record=True) as w:
gffutils.create_db(
gffutils.example_filename("FBgn0031208.gtf"),
":memory:",
infer_gene_extent=False,
)
assert len(w) == 1
def test_for_analyze():
db = gffutils.create_db(
gffutils.example_filename("FBgn0031208.gtf"), "deleteme", force=True
)
assert db._analyzed()
db.execute("DROP TABLE sqlite_stat1")
assert not db._analyzed()
with warnings.catch_warnings(record=True) as w:
warnings.simplefilter("always")
db2 = gffutils.FeatureDB("deleteme")
assert len(w) == 1
assert "analyze" in str(w[-1].message)
db.analyze()
assert db._analyzed()
os.unlink("deleteme")
def test_sequence():
fasta = gffutils.example_filename("dm6-chr2L.fa")
f = feature.feature_from_line("chr2L FlyBase gene 154 170 . + . ID=one;")
seq = f.sequence(fasta)
assert seq == "aCGAGATGATAATATAT"
assert len(seq) == len(f)
f.strand = "-"
seq = f.sequence(fasta)
assert seq == "ATATATTATCATCTCGt"
assert len(seq) == len(f)
def test_unquoting():
# incoming is encoded
s = (
"chr1\tAUGUSTUS\tgene\t6950084\t6951407\t0.26\t-\t.\t"
"ID=INIL01g00009;GeneSymbol=Ndufaf6;Note=NADH dehydrogenase "
"(ubiquinone) complex I%2C assembly factor 6;GO_Terms=GO:0005743|"
"GO:0016740|GO:0009058|GO:0032981;PFam=PF00494"
)
f = feature.feature_from_line(s, keep_order=True)
# string representation should be identical
assert str(f) == s
# accessing attribute should be decoded
n = f["Note"]
assert n == ["NADH dehydrogenase (ubiquinone) complex I, assembly factor 6"]
def test_unreasonable_unquoting():
s = (
"chr1\t.\t.\t1\t2\t0.26\t-\t.\t"
"newline=%0A;"
"percent=%25;"
"null=%00;"
"comma=%2C;"
# The first parent is "A," (A with a comma), the second is "B%"
"Parent=A%2C,B%25,C;"
)
f = feature.feature_from_line(s, keep_order=True)
assert f.attributes["newline"][0] == "\n"
assert f.attributes["percent"][0] == "%"
assert f.attributes["null"][0] == "\x00"
assert f.attributes["comma"][0] == ","
# Commas indicate
assert f.attributes["Parent"] == ["A,", "B%", "C"]
assert str(f) == s
def test_unquoting_iter():
s = "chr1\t.\tgene\t1\t2\t.\t-\t.\tID=%2C;"
tmp = tempfile.NamedTemporaryFile(delete=False).name
with open(tmp, "w") as fout:
fout.write(s + "\n")
assert list(gffutils.iterators.DataIterator(tmp))[0]["ID"][0] == ","
def test_db_unquoting():
s = dedent(
"""
chr1\t.\tgene\t1\t2\t.\t-\t.\tID=a;Note=%2C;
chr1\t.\tgene\t1\t2\t.\t-\t.\tID=b;Note=%2C;
chr1\t.\tgene\t1\t2\t.\t-\t.\tID=c;Note=%2C;
chr1\t.\tgene\t1\t2\t.\t-\t.\tID=d;Note=%2C;
chr1\t.\tgene\t1\t2\t.\t-\t.\tID=e;Note=%2C;
chr1\t.\tgene\t1\t2\t.\t-\t.\tID=f;Note=%2C;
"""
)
tmp = tempfile.NamedTemporaryFile(delete=False).name
with open(tmp, "w") as fout:
fout.write(s + "\n")
db = gffutils.create_db(tmp, ":memory:", checklines=1)
assert db["a"]["Note"] == [","]
assert db["b"]["Note"] == [","]
assert db["c"]["Note"] == [","]
assert db["d"]["Note"] == [","]
assert db["e"]["Note"] == [","]
assert db["f"]["Note"] == [","]
def test_create_splice_sites():
fn = gffutils.example_filename("gff_example1.gff3")
db = gffutils.create_db(fn, ":memory:")
db = db.update(db.create_splice_sites())
observed = "\n".join(str(feature) for feature in db.all_features())
expected = dedent(
"""\
chr1 ensGene gene 4763287 4775820 . - . Name=ENSMUSG00000033845;ID=ENSMUSG00000033845;Alias=ENSMUSG00000033845;gid=ENSMUSG00000033845
chr1 ensGene mRNA 4764517 4775779 . - . Name=ENSMUST00000045689;Parent=ENSMUSG00000033845;ID=ENSMUST00000045689;Alias=ENSMUSG00000033845;gid=ENSMUSG00000033845
chr1 ensGene CDS 4775654 4775758 . - 0 Name=ENSMUST00000045689.cds0;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.cds0;gid=ENSMUSG00000033845
chr1 ensGene CDS 4772761 4772814 . - 0 Name=ENSMUST00000045689.cds1;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.cds1;gid=ENSMUSG00000033845
chr1 ensGene exon 4775654 4775779 . - . Name=ENSMUST00000045689.exon0;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.exon0;gid=ENSMUSG00000033845
chr1 ensGene exon 4772649 4772814 . - . Name=ENSMUST00000045689.exon1;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.exon1;gid=ENSMUSG00000033845
chr1 ensGene exon 4767606 4767729 . - . Name=ENSMUST00000045689.exon2;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.exon2;gid=ENSMUSG00000033845
chr1 ensGene exon 4764517 4764597 . - . Name=ENSMUST00000045689.exon3;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.exon3;gid=ENSMUSG00000033845
chr1 ensGene five_prime_UTR 4775759 4775779 . - . Name=ENSMUST00000045689.utr0;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.utr0;gid=ENSMUSG00000033845
chr1 ensGene three_prime_UTR 4772649 4772760 . - . Name=ENSMUST00000045689.utr1;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.utr1;gid=ENSMUSG00000033845
chr1 ensGene three_prime_UTR 4767606 4767729 . - . Name=ENSMUST00000045689.utr2;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.utr2;gid=ENSMUSG00000033845
chr1 ensGene three_prime_UTR 4764517 4764597 . - . Name=ENSMUST00000045689.utr3;Parent=ENSMUST00000045689;ID=ENSMUST00000045689.utr3;gid=ENSMUSG00000033845
chr1 gffutils_derived three_prime_cis_splice_site 4764598 4764599 . - . Name=ENSMUST00000045689.exon2,ENSMUST00000045689.exon3;Parent=ENSMUST00000045689;ID=three_prime_cis_splice_site_ENSMUST00000045689.exon2-ENSMUST00000045689.exon3;gid=ENSMUSG00000033845
chr1 gffutils_derived three_prime_cis_splice_site 4767730 4767731 . - . Name=ENSMUST00000045689.exon1,ENSMUST00000045689.exon2;Parent=ENSMUST00000045689;ID=three_prime_cis_splice_site_ENSMUST00000045689.exon1-ENSMUST00000045689.exon2;gid=ENSMUSG00000033845
chr1 gffutils_derived three_prime_cis_splice_site 4772815 4772816 . - . Name=ENSMUST00000045689.exon0,ENSMUST00000045689.exon1;Parent=ENSMUST00000045689;ID=three_prime_cis_splice_site_ENSMUST00000045689.exon0-ENSMUST00000045689.exon1;gid=ENSMUSG00000033845
chr1 gffutils_derived five_prime_cis_splice_site 4767604 4767605 . - . Name=ENSMUST00000045689.exon2,ENSMUST00000045689.exon3;Parent=ENSMUST00000045689;ID=five_prime_cis_splice_site_ENSMUST00000045689.exon2-ENSMUST00000045689.exon3;gid=ENSMUSG00000033845
chr1 gffutils_derived five_prime_cis_splice_site 4772647 4772648 . - . Name=ENSMUST00000045689.exon1,ENSMUST00000045689.exon2;Parent=ENSMUST00000045689;ID=five_prime_cis_splice_site_ENSMUST00000045689.exon1-ENSMUST00000045689.exon2;gid=ENSMUSG00000033845
chr1 gffutils_derived five_prime_cis_splice_site 4775652 4775653 . - . Name=ENSMUST00000045689.exon0,ENSMUST00000045689.exon1;Parent=ENSMUST00000045689;ID=five_prime_cis_splice_site_ENSMUST00000045689.exon0-ENSMUST00000045689.exon1;gid=ENSMUSG00000033845"""
)
assert observed == expected
if __name__ == "__main__":
# this test case fails
# test_attributes_modify()
# test_sanitize_gff()
# test_random_chr()
# test_nonascii()
test_iterator_update()
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