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#################################################################################
# Copyright (c) 2011-2015, Pacific Biosciences of California, Inc.
#
# All rights reserved.
#
# Redistribution and use in source and binary forms, with or without
# modification, are permitted provided that the following conditions are met:
# * Redistributions of source code must retain the above copyright
# notice, this list of conditions and the following disclaimer.
# * Redistributions in binary form must reproduce the above copyright
# notice, this list of conditions and the following disclaimer in the
# documentation and/or other materials provided with the distribution.
# * Neither the name of Pacific Biosciences nor the names of its
# contributors may be used to endorse or promote products derived from
# this software without specific prior written permission.
#
# NO EXPRESS OR IMPLIED LICENSES TO ANY PARTY'S PATENT RIGHTS ARE GRANTED BY
# THIS LICENSE. THIS SOFTWARE IS PROVIDED BY PACIFIC BIOSCIENCES AND ITS
# CONTRIBUTORS "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
# LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A
# PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL PACIFIC BIOSCIENCES OR
# ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
# EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO,
# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR
# BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER
# IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE)
# ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE
# POSSIBILITY OF SUCH DAMAGE.
#################################################################################
# Author: David Alexander
"""
I/O support for FASTQ files
"""
__all__ = [ "FastqRecord",
"FastqReader",
"FastqWriter",
"qvsFromAscii",
"asciiFromQvs" ]
import numpy as np
from .base import ReaderBase, WriterBase
from .FastaIO import splitFastaHeader
from pbcore import sequence
from pbcore.util.decorators import deprecated
class FastqRecord(object):
"""
A ``FastqRecord`` object models a named sequence and its quality
values in a FASTQ file. For reference consult `Wikipedia's FASTQ
entry`_. We adopt the Sanger encoding convention, allowing the
encoding of QV values in [0, 93] using ASCII 33 to 126. We only
support FASTQ files in the four-line convention (unwrapped).
Wrapped FASTQ files are generally considered a bad idea as the @,
+ delimiters can also appear in the quality string, thus parsing
cannot be done safely.
.. _Wikipedia's FASTQ entry: http://en.wikipedia.org/wiki/FASTQ_format
"""
DELIMITER1 = "@"
DELIMITER2 = "+"
def __init__(self, header, sequence, quality=None, qualityString=None):
try:
assert "\n" not in header
assert "\n" not in sequence
self._header = header
self._sequence = sequence
self._id, self._comment = splitFastaHeader(header)
# Only one of quality, qualityString should be provided
assert (quality is None) != (qualityString is None)
if quality is not None:
self._quality = quality
else:
self._quality = qvsFromAscii(qualityString)
assert len(self.sequence) == len(self.quality)
except AssertionError:
raise ValueError("Invalid FASTQ record data")
@property
def header(self):
"""
The header of the sequence in the FASTQ file
"""
return self._header
@property
def name(self):
"""
DEPRECATED: The name of the sequence in the FASTQ file
"""
return self._header
@property
def id(self):
"""
The id of the sequence in the FASTQ file, equal to the FASTQ header
up to the first whitespace.
"""
return self._id
@property
@deprecated
def length(self):
"""
The length of the sequence
"""
return len(self.sequence)
@property
def comment(self):
"""
The comment associated with the sequence in the FASTQ file, equal to
the contents of the FASTQ header following the first whitespace
"""
return self._comment
@property
def sequence(self):
"""
The sequence for the record as present in the FASTQ file.
"""
return self._sequence
@property
def quality(self):
"""
The quality values, as an array of integers
"""
return self._quality
@property
def qualityString(self):
"""
The quality values as an ASCII-encoded string
"""
return asciiFromQvs(self._quality)
@classmethod
def fromString(cls, s):
"""
Interprets a string as a FASTQ record. Only supports four-line
format, as wrapped FASTQs can't easily be safely parsed.
"""
try:
lines = s.rstrip().splitlines()
assert len(lines) == 4
assert lines[0][0] == cls.DELIMITER1
assert lines[2][0] == cls.DELIMITER2
assert len(lines[1]) == len(lines[3])
header = lines[0][1:]
sequence = lines[1]
quality = qvsFromAscii(lines[3])
return FastqRecord(header, sequence, quality)
except AssertionError:
raise ValueError("String not recognized as a valid FASTQ record")
def reverseComplement(self, preserveHeader=False):
"""
Return a new FastaRecord with the reverse-complemented DNA sequence.
Optionally, supply a name
"""
rcSequence = sequence.reverseComplement(self.sequence)
rcQuality = sequence.reverse(self.quality)
if preserveHeader:
return FastqRecord(self.header, rcSequence, rcQuality)
else:
rcName = '{0} [revcomp]'.format(self.header.strip())
return FastqRecord(rcName, rcSequence, rcQuality)
def __len__(self):
return len(self._sequence)
def __eq__(self, other):
if isinstance(other, self.__class__):
return (self.header == other.header and
self.sequence == other.sequence and
np.array_equiv(self.quality, other.quality))
else:
return False
def __ne__(self, other):
return not self.__eq__(other)
def __str__(self):
"""
Output a string representation of this FASTQ record, in
standard four-line format.
"""
return "\n".join([self.DELIMITER1 + self.header,
self.sequence,
self.DELIMITER2,
self.qualityString])
class FastqReader(ReaderBase):
"""
Reader for FASTQ files, useable as a one-shot iterator over
FastqRecord objects. FASTQ files must follow the four-line
convention.
"""
def __iter__(self):
"""
One-shot iteration support
"""
while True:
lines = [next(self.file) for i in xrange(4)]
yield FastqRecord(lines[0][1:-1],
lines[1][:-1],
qualityString=lines[3][:-1])
class FastqWriter(WriterBase):
"""
A FASTQ file writer class
Example:
.. doctest::
>>> from pbcore.io import FastqWriter
>>> with FastqWriter("output.fq.gz") as writer:
... writer.writeRecord("dog", "GATTACA", [35]*7)
... writer.writeRecord("cat", "CATTACA", [35]*7)
.. testcleanup::
import os; os.unlink("output.fq.gz")
(Notice that underlying file will be automatically closed after
exit from the `with` block.)
"""
def writeRecord(self, *args):
"""
Write a FASTQ record to the file. If given one argument, it is
interpreted as a ``FastqRecord``. Given three arguments, they
are interpreted as the name, sequence, and quality.
"""
if len(args) not in (1, 3):
raise ValueError
if len(args) == 1:
record = args[0]
assert isinstance(record, FastqRecord)
else:
header, sequence, quality = args
record = FastqRecord(header, sequence, quality)
self.file.write(str(record))
self.file.write("\n")
##
## Utility
##
def qvsFromAscii(s):
return (np.fromstring(s, dtype=np.uint8) - 33)
def asciiFromQvs(a):
return (np.clip(a, 0, 93).astype(np.uint8) + 33).tostring()
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