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#!/usr/bin/env python
# File created on 09 Feb 2010
from __future__ import division
__author__ = "Rob Knight"
__copyright__ = "Copyright 2011, The QIIME Project"
__credits__ = ["Rob Knight", "Daniel McDonald", "Kyle Bittinger"]
__license__ = "GPL"
__version__ = "1.4.0"
__maintainer__ = "Kyle Bittinger"
__email__ = "kylebittinger@gmail.com"
__status__ = "Release"
from qiime.util import make_option
from qiime.make_per_library_sff import make_per_library_sffs
from qiime.util import parse_command_line_parameters
#make_per_library_sff.py
script_info={}
script_info['brief_description']="""Make per-library sff files from ID lists"""
script_info['script_description']="""This script generates per-library sff files using a directory of text files, one per library, which list read ID's to be included.
The ID list files should contain one read ID per line. If a line contains multiple words (separated by whitespace), then only the first word is used. A '>' character is stripped from the beginning of the line, if present. Blank lines in the file are skipped.
"""
script_info['script_usage']=[]
script_info['script_usage'].append(("""Example:""","""Make per-library sff files using input.sff and a directory of libs where each file in the directory contains the id lists for each library:""","""make_per_library_sff.py -i input.sff -l libs"""))
script_info['output_description']="""The result of this script generates sff files for each library."""
script_info['required_options'] = [
make_option("-i", "--input_sff",
help="Input sff file (separate multiple files w/ comma)"),
make_option("-l", "--libdir",
help="Directory containing ID list text files, one per library"),
]
script_info['optional_options'] = [
make_option("-p", "--sfffile_path",
help="Path to sfffile binary [default: use sfffile in $PATH]"),
make_option('--use_sfftools', action='store_true', default=False,
help=('Use external sfffile program instead of equivalent Python '
'routines.')),
make_option('--debug', action='store_true', default=False,
help="Print debugging output to stdout [default: %default]"),
]
script_info['version'] = __version__
def main():
option_parser, opts, args = parse_command_line_parameters(**script_info)
sff_fps = opts.input_sff.split(',')
make_per_library_sffs(
sff_fps,
opts.libdir,
opts.use_sfftools,
opts.sfffile_path,
opts.debug,
)
if __name__ == "__main__":
main()
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