File: parallel_beta_diversity.py

package info (click to toggle)
qiime 1.4.0-2
  • links: PTS, VCS
  • area: main
  • in suites: wheezy
  • size: 29,704 kB
  • sloc: python: 77,837; haskell: 379; sh: 113; makefile: 103
file content (243 lines) | stat: -rwxr-xr-x 11,078 bytes parent folder | download
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
#!/usr/bin/env python
# File created on 09 Feb 2010
from __future__ import division

__author__ = "Greg Caporaso"
__copyright__ = "Copyright 2011, The QIIME Project"
__credits__ = ["Greg Caporaso","Justin Kuczynski"]
__license__ = "GPL"
__version__ = "1.4.0"
__maintainer__ = "Greg Caporaso"
__email__ = "gregcaporaso@gmail.com"
__status__ = "Release"

from qiime.util import parse_command_line_parameters
from qiime.util import make_option
from optparse import OptionParser
from glob import glob
from os import popen, makedirs
from os.path import split, splitext, join, isfile
from subprocess import check_call, CalledProcessError
from qiime.util import get_tmp_filename
from qiime.parallel.util import get_random_job_prefix, write_jobs_file,\
    submit_jobs, get_rename_command,\
    write_filepaths_to_file, merge_to_n_commands
from qiime.beta_diversity import get_phylogenetic_metric
from qiime.util import load_qiime_config, get_qiime_scripts_dir, get_options_lookup
from qiime.parallel.beta_diversity import (get_job_commands_single_otu_table,
    get_job_commands_multiple_otu_tables, create_merge_map_file_single_otu_table,
    get_poller_command,commands_to_shell_script)
from qiime.parse import parse_otu_table, parse_newick, PhyloNode
from sys import stderr

qiime_config = load_qiime_config()
options_lookup = get_options_lookup()

script_info={}
script_info['brief_description']="""Parallel beta diversity"""
script_info['script_description']="""This script performs like the beta_diversity.py script, but is intended to make use of multicore/multiprocessor environments to perform analyses in parallel."""
script_info['script_usage']=[]
script_info['script_usage'].append(("""Example""","""Apply the dist_unweighted_unifrac and the dist_weighted_unifrac metrics (-m) to all otu tables in /home/qiime_user/rare/ (-i) and write the resulting output files to /home/qiime_user/out/ (-o, will be created if it doesn't exist). Use the tree file /home/qiime_user/rep_set.tre (-t) when necessary.""","""%prog -i /home/qiime_user/rare/ -o /home/qiime_user/out -m dist_unweighted_unifrac,dist_weighted_unifrac -t /home/qiime_user/rep_set.tre"""))

script_info['output_description']="""The output of %prog is a folder containing text files, each a distance matrix between samples."""

script_info['required_options'] = [\
 make_option('-i', '--input_path',
        help='input path, must be directory [REQUIRED]'),\
 make_option('-o', '--output_path',
        help='output path, must be directory [REQUIRED]'),
]

script_info['optional_options'] = [
 make_option('-m', '--metrics', default='unweighted_unifrac,weighted_unifrac',
     help='Beta-diversity metric(s) to use. A comma-separated list should be' +\
     ' provided when multiple metrics are specified. [default: %default]'),
 make_option('-t', '--tree_path', default=None,
        help='path to newick tree file, required for phylogenetic metrics'+\
        ' [default: %default]'),\
 make_option('-N','--beta_diversity_fp',action='store',\
           type='string',help='full path to '+\
           'scripts/beta_diversity.py [default: %default]',\
           default=join(get_qiime_scripts_dir(),'beta_diversity.py')),\
 options_lookup['poller_fp'],\
 options_lookup['retain_temp_files'],\
 options_lookup['suppress_submit_jobs'],\
 options_lookup['poll_directly'],\
 options_lookup['cluster_jobs_fp'],\
 options_lookup['suppress_polling'],\
 options_lookup['job_prefix'],\
 options_lookup['python_exe_fp'],\
 options_lookup['seconds_to_sleep'],\
 options_lookup['jobs_to_start'],
 make_option('-f', '--full_tree', action="store_true",
     help='By default, each job removes calls _fast_unifrac_setup to remove unused parts of the tree. pass -f if you already have a minimal tree, and this script will run faster'),

]
script_info['version'] = __version__

def main():
    option_parser, opts, args = parse_command_line_parameters(**script_info)
    
    # create local copies of command-line options
    input_path = opts.input_path
    output_dir = opts.output_path
    metrics = opts.metrics
    tree_fp = opts.tree_path

    # Check the tree exists if phylogenetically-aware measure is used
    metrics_list = metrics.split(',')
    for metric in metrics_list:
        try:
            metric_f = get_phylogenetic_metric(metric)
            if tree_fp == None:
                stderr.write("metric %s requires a tree, but none found\n"\
                    % (metric,))
                exit(1)
        except AttributeError:
            pass        

    beta_diversity_fp = opts.beta_diversity_fp
    python_exe_fp = opts.python_exe_fp
    path_to_cluster_jobs = opts.cluster_jobs_fp
    poller_fp = opts.poller_fp
    retain_temp_files = opts.retain_temp_files
    suppress_polling = opts.suppress_polling
    seconds_to_sleep = opts.seconds_to_sleep
    poll_directly = opts.poll_directly
    jobs_to_start = opts.jobs_to_start
    
    if isfile(input_path):
        single_otu_table_mode = True
    else:
        single_otu_table_mode = False
        input_fps = glob('%s/*' % input_path)

    created_temp_paths = []
    # split the input filepath into directory and filename, base filename and
    # extension
    # input_path, input_fn = split(input_path)
    # input_file_basename, input_file_ext = splitext(input_fn)
    
    # set the job_prefix either based on what the user passed in,
    # or a random string beginning with BDIV
    job_prefix = opts.job_prefix or get_random_job_prefix('BDIV')
    
    # A temporary output directory is created in output_dir named
    # job_prefix. Output files are then moved from the temporary 
    # directory to the output directory when they are complete, allowing
    # a poller to detect when runs complete by the presence of their
    # output files.
    working_dir = '%s/%s' % (output_dir,job_prefix)
    try:
        makedirs(working_dir)
        created_temp_paths.append(working_dir)
    except OSError:
        # working dir already exists
        pass
    
    # build the filepath for the 'jobs script'
    jobs_fp = '%s/%sjobs.txt' % (output_dir, job_prefix)
    created_temp_paths.append(jobs_fp)
    
    # Get the list of commands to be run and the expected result files
    if single_otu_table_mode:
        # these will be the row dissim matrices
        # temp for making, then move to output/i so the poller knows we're done
        for i in range(jobs_to_start):
            makedirs(working_dir + '/' + str(i))
            created_temp_paths.append(working_dir + '/' + str(i))
            makedirs(output_dir + '/' + str(i))
            created_temp_paths.append(output_dir + '/' + str(i))

        # to speed up this process, if not opts.full_tree: call setup here once
        # and then use full_tree=True
        # not implemented yet
        commands, job_result_filepaths  = \
         get_job_commands_single_otu_table(python_exe_fp,beta_diversity_fp,
         tree_fp,job_prefix,metrics,input_path,output_dir,working_dir,
         jobs_to_start,command_prefix=' ',command_suffix=' ',
         full_tree=opts.full_tree)
        created_temp_paths += job_result_filepaths
    else:
        commands, job_result_filepaths  = \
         get_job_commands_multiple_otu_tables(python_exe_fp,beta_diversity_fp,
         tree_fp,job_prefix,metrics,input_fps,output_dir,working_dir,
         command_prefix=' ',command_suffix=' ', full_tree=opts.full_tree)
        # Merge commands into jobs_to_start number of jobs
        commands = merge_to_n_commands(commands,jobs_to_start)
        
    # Set up poller apparatus if the user does not suppress polling
    if not suppress_polling:
        # Write the list of files which must exist for the jobs to be 
        # considered complete
        expected_files_filepath = '%s/expected_out_files.txt' % working_dir
        write_filepaths_to_file(job_result_filepaths,expected_files_filepath)
        created_temp_paths.append(expected_files_filepath)
        
        # Write the mapping file which described how the output files from
        # each job should be merged into the final output files
        merge_map_filepath = '%s/merge_map.txt' % working_dir
        if single_otu_table_mode:
            create_merge_map_file_single_otu_table(
             input_path,output_dir,metrics,merge_map_filepath,
             expected_files_filepath)
            process_run_results_f =\
             'qiime.parallel.beta_diversity.parallel_beta_diversity_process_run_results_f'
        else:
            open(merge_map_filepath,'w').close()
            process_run_results_f = None
        created_temp_paths.append(merge_map_filepath)

        # Create the filepath listing the temporary files to be deleted,
        # but don't write it yet
        deletion_list_filepath = '%s/deletion_list.txt' % working_dir
        created_temp_paths.append(deletion_list_filepath)

        if not poll_directly:
            # Generate the command to run the poller, and the list of temp files
            # created by the poller
            poller_command, poller_result_filepaths =\
             get_poller_command(python_exe_fp,poller_fp,expected_files_filepath,
             merge_map_filepath,deletion_list_filepath,
             process_run_results_f=process_run_results_f,
             seconds_to_sleep=seconds_to_sleep,
             command_prefix='',command_suffix='')
            # append the poller command to the list of job commands
            poller_shell_script_fp = '%s/poller.sh' % working_dir
            commands_to_shell_script([poller_command],poller_shell_script_fp)
            commands.append('bash %s' % poller_shell_script_fp)
        else:
            poller_command, poller_result_filepaths =\
             get_poller_command(python_exe_fp,poller_fp,expected_files_filepath,
             merge_map_filepath,deletion_list_filepath,
             seconds_to_sleep=seconds_to_sleep,
             process_run_results_f=process_run_results_f,
             command_prefix='',command_suffix='')            
        created_temp_paths += poller_result_filepaths
        if not retain_temp_files:
            # If the user wants temp files deleted, now write the list of 
            # temp files to be deleted
            write_filepaths_to_file(created_temp_paths,deletion_list_filepath)
        else:
            # Otherwise just write an empty file
            write_filepaths_to_file([],deletion_list_filepath)
    
    # write the commands to the 'jobs files'
    write_jobs_file(commands,job_prefix=job_prefix,jobs_fp=jobs_fp)
    
    # submit the jobs file using cluster_jobs, if not suppressed by the
    # user
    if not opts.suppress_submit_jobs:
        submit_jobs(path_to_cluster_jobs,jobs_fp,job_prefix)
        
    if poll_directly:
        try:
            check_call(poller_command.split())
        except CalledProcessError, e:
            print '**Error occuring when calling the poller directly. '+\
            'Jobs may have been submitted, but are not being polled.'
            print str(e)
            exit(-1)

if __name__ == "__main__":
    main()