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### -----------------------------------------------------------------
### chromosome to colour mapping
### Not exported!
chr2colour <- function(chromosomes){
### diverging colour scheme from http://colorbrewer2.org
divergingColour <- c("#9e0142", "#d53e4f", "#f46d43", "#fdae61", "#fee08b",
"#ffffbf", "#e6f598", "#abdda4", "#66c2a5", "#3288bd",
"#5e4fa2")
uniqChromosomes <- as.character(unique(chromosomes))
chrColours <- rep(divergingColour,
ceiling(length(uniqChromosomes) / length(divergingColour)))
mapping <- chrColours[1:length(uniqChromosomes)]
names(mapping) <- uniqChromosomes
return(mapping[as.character(chromosomes)])
}
### -----------------------------------------------------------------
### readAncoraCNEs: this is for internal use in Lenhard group.
### It reads the CNE file (filename format: cne2wBf_hg38_mm10_50_50)
### and return a GRanges.
### Exported!
readAncora <- function(fn, assembly=NULL,
tAssemblyFn=NULL, qAssemblyFn=NULL){
assembly1 <- strsplit(basename(fn), split="_")[[1]][2]
assembly2 <- strsplit(basename(fn), split="_")[[1]][3]
cne <- read.delim(fn, header=FALSE)
# Prepare the seqinfo when available
seqinfoTarget <- NULL
if(!is.null(tAssemblyFn)){
seqinfoTarget <- seqinfoFn(tAssemblyFn)
}
seqinfoQuery <- NULL
if(!is.null(qAssemblyFn)){
seqinfoQuery <- seqinfoFn(qAssemblyFn)
}
ans <- GRangePairs(first=GRanges(seqnames=cne[[1]],
ranges=IRanges(start=cne[[2]]+1,
end=cne[[3]]),
strand="*",
name=paste0(cne[[4]], ":",
(cne[[5]]+1), "-", cne[[6]]),
itemRgb=chr2colour(cne[[4]]),
seqnames2=cne[[4]],
seqinfo=seqinfoTarget),
second=GRanges(seqnames=cne[[4]],
ranges=IRanges(start=cne[[5]]+1,
end=cne[[6]]),
strand="*",
name=paste0(cne[[1]], ":",
(cne[[2]]+1), "-", cne[[3]]),
itemRgb=chr2colour(cne[[1]]),
seqnames2=cne[[1]],
seqinfo=seqinfoQuery)
)
if(is.null(assembly)){
## Real both assemblies
return(ans)
}else if(assembly == assembly1){
## Read first assembly
return(first(ans))
}else if(assembly == assembly2){
## Read last assembly
return(second(ans))
}else{
stop("Wrongly specified assembly!")
}
}
### -----------------------------------------------------------------
### readAncoraIntoSQLite: read the Ancora format CNE files into a SQLite
### Exported!
readAncoraIntoSQLite <- function(cneFns, dbName, overwrite=FALSE){
tableNames <- c()
for(cneFn in cneFns){
## cneFn in format of cne2wBf_AstMex102_danRer10_48_50
tableName <- sub("^cne2wBf_", "", basename(cneFn))
tableNames <- c(tableNames, tableName)
df <- readAncora(cneFn, assembly=NULL)
saveCNEToSQLite(df, dbName=dbName, tableName=tableName,
overwrite=overwrite)
}
invisible(tableNames)
}
### -----------------------------------------------------------------
### makeCNEDensity: make the Ancora downloads-like bed files, bedgraph,
### bigwig files from a GrangePairs of CNEs.
### Exported!
makeCNEDensity <- function(x, outputDir=".",
genomeFirst="first", genomeSecond="second",
threshold="50_50",
windowSizeFirst=300, ## kb
windowSizeSecond=300 ## kb
){
if(!is(x, "GRangePairs")){
stop("`x` must be a GRangePairs object!")
}
if(seqlengthsNA(x)){
stop("seqlengths must be provided in `x`!")
}
if(length(x) == 0L){
warning("No CNEs in `x`!")
return(FALSE)
}
## Always try to create the output folder
dir.create(outputDir, showWarnings=FALSE, recursive=TRUE)
## make the bed files
message("Making bed files...")
bedFirst <- first(x)
bedSecond <- second(x)
mcols(bedFirst) <- DataFrame(name=as.character(bedSecond),
score=0,
itemRgb=chr2colour(as.character(
seqnames(bedSecond)))
)
mcols(bedSecond) <- DataFrame(name=as.character(bedFirst),
score=0,
itemRgb=chr2colour(as.character(
seqnames(bedFirst)))
)
firstTrackLine <- new("BasicTrackLine", itemRgb=TRUE,
name=paste(genomeFirst, "CNEs", threshold),
description=paste(genomeFirst, "CNEs", threshold)
)
secondTrackLine <- new("BasicTrackLine", itemRgb=TRUE,
name=paste(genomeSecond, "CNEs", threshold),
description=paste(genomeSecond, "CNEs", threshold)
)
bedFnFirst <- file.path(outputDir,
paste0("CNE_", genomeFirst, "_",
genomeSecond, "_",
threshold, ".bed"))
export.bed(bedFirst, con=bedFnFirst,
trackLine=firstTrackLine)
bedFnSecond <- file.path(outputDir,
paste0("CNE_", genomeSecond, "_",
genomeFirst, "_",
threshold, ".bed"))
export.bed(bedSecond, con=bedFnSecond,
trackLine=secondTrackLine)
# Make the bedGraph files
message("Making bedGraph files...")
bedFirst <- reduce(bedFirst, ignore.strand=TRUE)
covFirst <- coverage(bedFirst)
densityFirst <- suppressWarnings(runmean(covFirst, k=windowSizeFirst*1000,
endrule = "constant") * 100)
bedSecond <- reduce(bedSecond, ignore.strand=TRUE)
covSecond <- coverage(bedSecond)
densitySecond <- suppressWarnings(runmean(covSecond, k=windowSizeSecond*1000,
endrule = "constant") * 100)
firstTrackLine <- new("GraphTrackLine",
name=paste(genomeFirst, "CNEs density", threshold),
description=paste(genomeFirst, "CNEs density",
threshold),
visibility="full", type="bedGraph", autoScale=TRUE
)
secondTrackLine <- new("GraphTrackLine",
name=paste(genomeSecond, "CNEs density", threshold),
description=paste(genomeSecond, "CNEs density",
threshold),
visibility="full", type="bedGraph", autoScale=TRUE
)
bwFnFirst <- file.path(outputDir,
paste0("CNE_density_", genomeFirst, "_",
genomeSecond, "_",
threshold, ".bedGraph"))
export.bedGraph(densityFirst, con=bwFnFirst, trackLine=firstTrackLine)
bwFnSecond <- file.path(outputDir,
paste0("CNE_density_", genomeSecond, "_",
genomeFirst, "_",
threshold, ".bedGraph"))
export.bedGraph(densitySecond, con=bwFnSecond, trackLine=secondTrackLine)
# Make bigwig files
message("Making bigwig files...")
bigwigFnFirst <- file.path(outputDir,
paste0("CNE_density_", genomeFirst, "_",
genomeSecond, "_", threshold, ".bw"))
export.bw(densityFirst, con=bigwigFnFirst)
bigwigFnSecond <- file.path(outputDir,
paste0("CNE_density_", genomeSecond, "_",
genomeFirst, "_", threshold, ".bw"))
export.bw(densitySecond, con=bigwigFnSecond)
invisible(c(bedFnFirst, bedFnSecond, bwFnFirst, bwFnSecond,
bigwigFnFirst, bigwigFnSecond))
}
### -----------------------------------------------------------------
### makeAncoraFiles: in the format of cne2wBf_GmorY1_dm6_21_30 for loading
### into Ancora.
### Exported!!!
makeAncoraFiles <- function(cne, outputDir=".",
genomeFirst="first", genomeSecond="second",
threshold="50_50"){
# cne is a GRangePairs object
Sys.setlocale("LC_COLLATE","C")
unsortedNames <- c(genomeFirst, genomeSecond)
sortedNames <- sort(c(genomeFirst, genomeSecond))
fileName <- paste("cne2wBf", paste(sortedNames, collapse="_"),
threshold, sep="_")
if(identical(sortedNames, unsortedNames)){
## The order is right
ans <- data.frame(seqnames(first(cne)),
start(first(cne))-1L, end(first(cne)),
seqnames(second(cne)),
start(second(cne))-1L, end(second(cne)),
strand(second(cne)),
mcols(cne)$score,
mcols(cne)$cigar)
}else{
## The order is not
ans <- data.frame(seqnames(second(cne)),
start(second(cne))-1L, end(second(cne)),
seqnames(first(cne)),
start(first(cne))-1L, end(first(cne)),
strand(first(cne)),
mcols(cne)$score,
mcols(cne)$cigar)
}
dir.create(outputDir, showWarnings=FALSE, recursive=TRUE)
write.table(ans, file=file.path(outputDir, fileName),
sep="\t", quote=FALSE, row.names=FALSE,
col.names=FALSE)
invisible(file.path(outputDir, fileName))
}
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