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# == title
# The AnnotationFunction Class
#
# == details
# The heatmap annotation is basically graphics aligned to the heatmap columns or rows.
# There is no restriction for the graphic types, e.g. it can be heatmap-like
# annotation or points. Here the AnnotationFunction class is designed for
# creating complex and flexible annotation graphics. As the main part of the class, it uses
# a user-defined function to define the graphics. It also keeps information of
# the size of the plotting regions of the annotation. And most importantly, it
# allows subsetting to the annotation to draw a subset of the graphics, which
# is the base for the splitting of the annotations.
#
# See `AnnotationFunction` constructor for details.
#
AnnotationFunction = setClass("AnnotationFunction",
slots = list(
which = "character",
fun_name = "character",
width = "ANY",
height = "ANY",
n = "ANY",
var_env = "environment",
fun = "function",
subset_rule = "list",
subsettable = "logical",
data_scale = "numeric",
extended = "ANY",
show_name = "logical"
),
prototype = list(
fun_name = "",
width = unit(1, "npc"),
height = unit(1, "npc"),
subset_rule = list(),
subsettable = FALSE,
data_scale = c(0, 1),
n = NA_integer_,
extended = unit(c(0, 0, 0, 0), "mm"),
show_name = TRUE
)
)
anno_width_and_height = function(which, width = NULL, height = NULL,
default = unit(10, "mm")) {
if(which == "column") {
if(is.null(height)) {
height = default
} else {
if(!is_abs_unit(height)) {
stop_wrap("height of the annotation can only be an absolute unit.")
} else {
# height = convertHeight(height, "mm")
}
}
if(is.null(width)) {
width = unit(1, "npc")
}
}
if(which == "row") {
if(is.null(width)) {
width = default
} else {
if(!is_abs_unit(width)) {
stop_wrap("width of the annotation can only be an absolute unit.")
} else {
# width = convertWidth(width, "mm")
}
}
if(is.null(height)) {
height = unit(1, "npc")
}
}
return(list(height = height, width = width))
}
# == title
# Constructor of AnnotationFunction Class
#
# == param
# -fun A function which defines how to draw the annotation. See **Details** section.
# -fun_name The name of the function. It is only used for printing the object.
# -which Whether it is drawn as a column annotation or a row annotation?
# -cell_fun A simplified version of ``fun``. ``cell_fun`` only accepts one single index and it draws repeatedly in each annotation cell.
# -var_import The names of the variables or the variable themselves that the annotation function depends on. See **Details** section.
# -n Number of observations in the annotation. It is not mandatory, but it is better to provide this information
# so that the higher order `HeatmapAnnotation` knows it and it can perform check on the consistency of annotations and heatmaps.
# -data_scale The data scale on the data axis (y-axis for column annotation and x-axis for row annotation). It is only used
# when `decorate_annotation` is used with "native" unit coordinates.
# -subset_rule The rule of subsetting variables in ``var_import``. It should be set when users want the final object to
# be subsettable. See **Details** section.
# -subsettable Whether the object is subsettable?
# -show_name It is used to turn off the drawing of annotation names in `HeatmapAnnotation`. Annotations always have names
# associated and normally they will be drawn beside the annotation graphics to tell what the annotation is about.
# e.g. the annotation names put beside the points annotation graphics. However, for some of the annotations, the names
# are not necessarily to be drawn, such as text annotations drawn by `anno_text` or an empty annotation drawn by `anno_empty`.
# In this case, when ``show_names`` is set to ``FALSE``, there will be no annotation names drawn for the annotation.
# -width The width of the plotting region (the viewport) that the annotation is drawn. If it is a row annotation,
# the width must be an absolute unit. Since the ``AnnotationFunction`` object is always contained by the `SingleAnnotation-class`object,
# you can only set the width of row annotations or height of column annotations, while e.g. the height of the row annotation is always ``unit(1, "npc")``
# which means it always fully filled in the parent ``SingleAnnotation`` and only in `SingleAnnotation` or even `HeatmapAnnotation`
# can adjust the height of the row annotations.
# -height The height of the plotting region (the viewport) that the annotation is drawn. If it is a column annotation,
# the width must be an absolute unit.
#
# == details
# In the package, we have implemted quite a lot annotation functions by `AnnotationFunction` constructor:
# `anno_empty`, `anno_image`, `anno_points`, `anno_lines`, `anno_barplot`, `anno_boxplot`, `anno_histogram`,
# `anno_density`, `anno_joyplot`, `anno_horizon`, `anno_text` and `anno_mark`. These built-in annotation functions
# support as both row annotations and column annotations and they are are all subsettable.
#
# The build-in annotation functions are already enough for most of the analysis, nevertheless, if users
# want to know more about how to construct the AnnotationFunction class manually, they can refer to
# https://jokergoo.github.io/ComplexHeatmap-reference/book/heatmap-annotations.html#implement-new-annotation-functions.
#
# == value
# A `AnnotationFunction-class` object which can be used in `HeatmapAnnotation`.
#
# == example
# x = 1:10
# anno1 = AnnotationFunction(
# fun = function(index, k, n) {
# n = length(index)
# pushViewport(viewport(xscale = c(0.5, n + 0.5), yscale = c(0, 10)))
# grid.rect()
# grid.points(1:n, x[index], default.units = "native")
# if(k == 1) grid.yaxis()
# popViewport()
# },
# var_import = list(x = x),
# n = 10,
# subsettable = TRUE,
# height = unit(2, "cm")
# )
# m = rbind(1:10, 11:20)
# Heatmap(m, top_annotation = HeatmapAnnotation(foo = anno1))
# Heatmap(m, top_annotation = HeatmapAnnotation(foo = anno1), column_km = 2)
AnnotationFunction = function(fun, fun_name = "", which = c("column", "row"), cell_fun = NULL,
var_import = list(), n = NA, data_scale = c(0, 1), subset_rule = list(),
subsettable = length(subset_rule) > 0, show_name = TRUE, width = NULL, height = NULL) {
which = match.arg(which)[1]
verbose = ht_opt$verbose
anno = new("AnnotationFunction")
anno@which = which
anno@fun_name = fun_name
if(verbose) qqcat("construct AnnotationFunction with '@{fun_name}()'\n")
anno_size = anno_width_and_height(which, width, height, unit(1, "cm"))
anno@width = anno_size$width
anno@height = anno_size$height
anno@show_name = show_name
anno@n = n
anno@data_scale = data_scale
if(!is.null(cell_fun)) {
if(which == "column") {
fun = function(index, k, n) {
n = length(index)
for(i in seq_along(index)) {
pushViewport(viewport(x = i/n, width = 1/n, just = "right", default.units = "npc"))
cell_fun(index[i])
popViewport()
}
}
} else {
fun = function(index, k, n) {
n = length(index)
for(i in seq_along(index)) {
pushViewport(viewport(y = 1 - i/n, height = 1/n, just = "bottom", default.units = "npc"))
cell_fun(index[i])
popViewport()
}
}
}
subsettable = TRUE
}
if(length(var_import) == 0) {
global_vars = codetools::findGlobals(fun, merge = FALSE)$variables
for(v in global_vars) {
var_import[[v]] = get(v, envir = environment(fun))
}
}
if(length(var_import)) {
anno@var_env = new.env()
if(is.character(var_import)) {
for(nm in var_import) {
anno@var_env[[nm]] = get(nm, envir = parent.frame())
}
} else if(inherits(var_import, "list")) {
if(is.null(names(var_import))) {
var_imported_nm = sapply(as.list(substitute(var_import))[-1], as.character)
names(var_import) = var_imported_nm
}
for(nm in names(var_import)) {
anno@var_env[[nm]] = var_import[[nm]]
}
} else {
stop_wrap("`var_import` needs to be a character vector which contains variable names or a list of variables.")
}
environment(fun) = anno@var_env
} else {
anno@var_env = environment(fun)
}
if(length(as.list(formals(fun))) == 1) {
formals(fun) = alist(index = , k = 1, n = 1)
}
anno@fun = fun
if(is.null(subset_rule)) {
for(nm in names(anno@var_env)) {
if(is.matrix(anno@var_env[[nm]])) {
anno@subset_rule[[nm]] = subset_matrix_by_row
} else if(inherits(anno@var_env[[nm]], "gpar")) {
anno@subset_rule[[nm]] = subset_gp
} else if(is.vector(anno@var_env[[nm]])) {
if(length(anno@var_env[[nm]]) > 1) {
anno@subset_rule[[nm]] = subset_vector
}
}
}
} else {
for(nm in names(subset_rule)) {
anno@subset_rule[[nm]] = subset_rule[[nm]]
}
}
if(missing(subsettable)) {
# is user defined subset rule
if(length(anno@subset_rule)) {
anno@subsettable = TRUE
}
} else {
anno@subsettable = subsettable
}
return(anno)
}
# == title
# Subset an AnnotationFunction Object
#
# == param
# -x An `AnnotationFunction-class` object.
# -i A vector of indices.
#
# == details
# One good thing for designing the `AnnotationFunction-class` is it can be subsetted,
# and this is the base for the splitting of the annotations.
#
# == example
# anno = anno_simple(1:10)
# anno[1:5]
# draw(anno[1:5], test = "subset of column annotation")
"[.AnnotationFunction" = function(x, i) {
if(nargs() == 1) {
return(x)
} else {
if(!x@subsettable) {
stop_wrap("This object is not subsettable.")
}
x = copy_all(x)
if(x@fun_name == "anno_mark") {
called_args = attr(x, "called_args")
od = order(i)
i = sort(i)
l = called_args$at %in% i
called_args$at = od[which(i %in% called_args$at)]
called_args$labels = called_args$labels[l]
called_args$link_gp = subset_gp(called_args$link_gp, l)
called_args$labels_gp = subset_gp(called_args$labels_gp, l)
x2 = do.call(anno_mark, called_args)
return(x2)
}
for(var in names(x@subset_rule)) {
oe = try(x@var_env[[var]] <- x@subset_rule[[var]](x@var_env[[var]], i), silent = TRUE)
if(inherits(oe, "try-error")) {
message(paste0("An error when subsetting ", var))
stop_wrap(oe)
}
}
if(is.logical(i)) {
x@n = sum(i)
} else {
x@n = length(i)
}
return(x)
}
}
# == title
# Draw the AnnotationFunction Object
#
# == param
# -object The `AnnotationFunction-class` object.
# -index Index of observations.
# -k Current slice index.
# -n Total number of slices.
# -test Is it in test mode? The value can be logical or a text which is plotted as the title of plot.
# -... Pass to `grid::viewport`.
#
# == detail
# Normally it is called internally by the `SingleAnnotation-class`.
#
# When ``test`` is set to ``TRUE``, the annotation graphic is directly drawn,
# which is generally for testing purpose.
#
setMethod(f = "draw",
signature = "AnnotationFunction",
definition = function(object, index, k = 1, n = 1, test = FALSE, ...) {
if(is.character(test)) {
test2 = TRUE
} else {
test2 = test
test = ""
}
if(test2) {
grid.newpage()
pushViewport(viewport(width = 0.8, height = 0.8))
if(is.na(object@n)) {
object@n = 1
}
}
verbose = ht_opt$verbose
if(verbose) qqcat("draw annotation generated by @{object@fun_name}\n")
if(missing(index)) index = seq_len(object@n)
anno_height = object@height
anno_width = object@width
# names should be passed to the data viewport
pushViewport(viewport(width = anno_width, height = anno_height, ...))
vp_name1 = current.viewport()$name
object@fun(index, k, n)
if(test2) {
grid.text(test, y = unit(1, "npc") + unit(2, "mm"), just = "bottom")
if(!identical(unit(0, "mm"), object@extended[1])) {
grid.rect(y = 1, height = unit(1, "npc") + object@extended[1], just = "top",
gp = gpar(fill = "transparent", col = "red", lty = 2))
} else if(!identical(unit(0, "mm"), object@extended[2])) {
grid.rect(x = 1, width = unit(1, "npc") + object@extended[2], just = "right",
gp = gpar(fill = "transparent", col = "red", lty = 2))
} else if(!identical(unit(0, "mm"), object@extended[3])) {
grid.rect(y = 0, height = unit(1, "npc") + object@extended[3], just = "bottom",
gp = gpar(fill = "transparent", col = "red", lty = 2))
} else if(!identical(unit(0, "mm"), object@extended[4])) {
grid.rect(x = 0, width = unit(1, "npc") + object@extended[4], just = "left",
gp = gpar(fill = "transparent", col = "red", lty = 2))
}
}
vp_name2 = current.viewport()$name
if(vp_name1 != vp_name2) {
stop_wrap("Viewports should be the same before and after plotting the annotation graphics.")
}
popViewport()
if(test2) {
popViewport()
}
})
# == title
# Copy the AnnotationFunction Object
#
# == param
# -object The `AnnotationFunction-class` object.
#
# == detail
# In `AnnotationFunction-class`, there is an environment which
# stores some external variables for the annotation function (specified by the
# ``var_import`` argument when constructing the `AnnotationFunction-class`
# object. This `copy_all,AnnotationFunction-method` hard copies all the
# variables into a new isolated environment.
#
# The environment is at ``object@var_env``.
#
setMethod(f = "copy_all",
signature = "AnnotationFunction",
definition = function(object) {
object2 = object
object2@var_env = new.env()
for(var in names(object@var_env)) {
object2@var_env[[var]] = object@var_env[[var]]
}
environment(object2@fun) = object2@var_env
return(object2)
})
# == title
# Print the AnnotationFunction Object
#
# == param
# -object The `AnnotationFunction-class` object.
#
setMethod(f = "show",
signature = "AnnotationFunction",
definition = function(object) {
cat("An AnnotationFunction object\n")
if(object@fun_name == "") {
cat(" function: user-defined\n")
} else {
cat(" function: ", object@fun_name, "()\n", sep = "")
}
cat(" position:", object@which, "\n")
cat(" items:", ifelse(object@n == 0, "unknown", object@n), "\n")
cat(" width:", as.character(object@width), "\n")
cat(" height:", as.character(object@height), "\n")
var_imported = names(object@var_env)
if(length(var_imported)) {
cat(" imported variable:", paste(var_imported, collapse = ", "), "\n")
var_subsettable = names(object@subset_rule)
if(length(var_subsettable)) {
cat(" subsettable variable:", paste(var_subsettable, collapse = ", "), "\n")
}
}
cat(" this object is ", ifelse(object@subsettable, "", "not "), "subsettable\n", sep = "")
dirt = c("bottom", "left", "top", "right")
for(i in 1:4) {
if(!identical(unit(0, "mm"), object@extended[i])) {
cat(" ", as.character(object@extended[i]), "extension on the", dirt[i], "\n")
}
}
})
# == title
# Number of Observations
#
# == param
# -object The `AnnotationFunction-class` object.
# -... Other arguments.
#
# == details It returns the ``n`` slot in the object. If it does not exist, it
# returns ``NA``.
#
# == example
# anno = anno_points(1:10)
# nobs(anno)
nobs.AnnotationFunction = function(object, ...) {
if(is.na(object@n)) {
return(NA)
} else if(object@n > 0) {
object@n
} else {
NA
}
}
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