1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499 500 501 502 503 504 505 506 507 508 509 510 511 512 513 514 515 516 517 518
|
# == title
# Translate pheatmap::pheatmap to ComplexHeatmap::Heatmap
#
# == param
# -mat The input matrix.
# -color The same as in `pheatmap::pheatmap`. Here you don't necessarily need to generate a long color vector.
# The discrete colors sent to `grDevices::colorRampPalette` are also OK here. E.g.
# ``colorRampPalette(rev(brewer.pal(n = 7, name = "RdYlBu")))(100)`` can be simply
# replaced as ``rev(brewer.pal(n = 7, name = "RdYlBu"))``.
# -kmeans_k The same as in `pheatmap::pheatmap`.
# -breaks The same as in `pheatmap::pheatmap`.
# -border_color The same as in `pheatmap::pheatmap`.
# -cellwidth The same as in `pheatmap::pheatmap`.
# -cellheight The same as in `pheatmap::pheatmap`.
# -scale The same as in `pheatmap::pheatmap`.
# -cluster_rows The same as in `pheatmap::pheatmap`.
# -cluster_cols The same as in `pheatmap::pheatmap`.
# -clustering_distance_rows The same as in `pheatmap::pheatmap`.
# -clustering_distance_cols The same as in `pheatmap::pheatmap`.
# -clustering_method The same as in `pheatmap::pheatmap`.
# -clustering_callback The same as in `pheatmap::pheatmap`.
# -cutree_rows The same as in `pheatmap::pheatmap`.
# -cutree_cols The same as in `pheatmap::pheatmap`.
# -treeheight_row The same as in `pheatmap::pheatmap`.
# -treeheight_col The same as in `pheatmap::pheatmap`.
# -legend The same as in `pheatmap::pheatmap`.
# -legend_breaks The same as in `pheatmap::pheatmap`.
# -legend_labels The same as in `pheatmap::pheatmap`.
# -annotation_row The same as in `pheatmap::pheatmap`.
# -annotation_col The same as in `pheatmap::pheatmap`.
# -annotation The same as in `pheatmap::pheatmap`.
# -annotation_colors The same as in `pheatmap::pheatmap`.
# -annotation_legend The same as in `pheatmap::pheatmap`.
# -annotation_names_row The same as in `pheatmap::pheatmap`.
# -annotation_names_col The same as in `pheatmap::pheatmap`.
# -drop_levels Enforced to be ``TRUE``.
# -show_rownames The same as in `pheatmap::pheatmap`.
# -show_colnames The same as in `pheatmap::pheatmap`.
# -main The same as in `pheatmap::pheatmap`.
# -fontsize The same as in `pheatmap::pheatmap`.
# -fontsize_row The same as in `pheatmap::pheatmap`.
# -fontsize_col The same as in `pheatmap::pheatmap`.
# -angle_col The same as in `pheatmap::pheatmap`.
# -display_numbers The same as in `pheatmap::pheatmap`.
# -number_format The same as in `pheatmap::pheatmap`.
# -number_color The same as in `pheatmap::pheatmap`.
# -fontsize_number The same as in `pheatmap::pheatmap`.
# -gaps_row The same as in `pheatmap::pheatmap`.
# -gaps_col The same as in `pheatmap::pheatmap`.
# -labels_row The same as in `pheatmap::pheatmap`.
# -labels_col The same as in `pheatmap::pheatmap`.
# -filename Not supported.
# -width Not supported.
# -height Not supported.
# -silent Not supported.
# -na_col The same as in `pheatmap::pheatmap`.
# -name Name of the heatmap. This argument is passed to `Heatmap`.
# -fontfamily Font family for row and column names.
# -fontfamily_row Font family for row names.
# -fontfamily_col Font family for column names.
# -fontface Font face for row and column names.
# -fontface_row Font face for row names.
# -fontface_col Font face for column names.
# -heatmap_legend_param Pass to `Heatmap`.
# -... Other arguments passed to `Heatmap`.
# -run_draw Whether to run ``draw()`` function to the heatmap object.
#
# == details
# This function aims to execute ``pheatmap::pheatmap`` code purely with ComplexHeatmap.
#
# == seealso
# See https://jokergoo.github.io/2020/05/06/translate-from-pheatmap-to-complexheatmap/
#
# `compare_pheatmap` that compares heatmaps between ``pheatmap::pheatmap()`` and ``ComplexHeatmap::pheatmap()``.
#
# == value
# A `Heatmap-class` object.
#
pheatmap = function(mat,
color = colorRampPalette(rev(brewer.pal(n = 7, name = "RdYlBu")))(100),
kmeans_k = NA,
breaks = NA,
border_color = ifelse(nrow(mat) < 100 & ncol(mat) < 100, "grey60", NA),
cellwidth = NA,
cellheight = NA,
scale = "none",
cluster_rows = TRUE,
cluster_cols = TRUE,
clustering_distance_rows = "euclidean",
clustering_distance_cols = "euclidean",
clustering_method = "complete",
clustering_callback = NA,
cutree_rows = NA,
cutree_cols = NA,
treeheight_row = ifelse(class(cluster_rows) == "hclust" || cluster_rows, 50, 0),
treeheight_col = ifelse(class(cluster_cols) == "hclust" || cluster_cols, 50, 0),
legend = TRUE,
legend_breaks = NA,
legend_labels = NA,
annotation_row = NA,
annotation_col = NA,
annotation = NA,
annotation_colors = NA,
annotation_legend = TRUE,
annotation_names_row = TRUE,
annotation_names_col = TRUE,
drop_levels = TRUE,
show_rownames = TRUE,
show_colnames = TRUE,
main = NA,
fontsize = 10,
fontsize_row = fontsize,
fontsize_col = fontsize,
angle_col = c("270", "0", "45", "90", "315"),
display_numbers = FALSE,
number_format = "%.2f",
number_color = "grey30",
fontsize_number = 0.8 * fontsize,
gaps_row = NULL,
gaps_col = NULL,
labels_row = NULL,
labels_col = NULL,
filename = NA,
width = NA,
height = NA,
silent = FALSE,
na_col = "#DDDDDD",
name = NULL,
# other graphic parameters for fonts
fontfamily = "",
fontfamily_row = fontfamily,
fontfamily_col = fontfamily,
fontface = 1,
fontface_row = fontface,
fontface_col = fontface,
# argument specific for Heatmap()
heatmap_legend_param = list(),
...,
run_draw = FALSE
) {
if(is.data.frame(mat)) {
warning_wrap("The input is a data frame, convert it to the matrix.")
mat = as.matrix(mat)
}
if(!identical(kmeans_k, NA)) {
warning_wrap("argument `kmeans_k` is not suggested to use in pheatmap -> Heatmap translation because it changes the input matrix. You might check `row_km` and `column_km` arguments in Heatmap().")
km = kmeans(mat, centers = kmeans_k)
mat = km$centers
rownames(mat) = paste0("Cluster: ", seq_along(km$size), ", Size: ", km$size)
}
if("row" %in% scale) {
if(any(is.na(mat))) {
mat = (mat - rowMeans(mat, na.rm = TRUE))/rowSds(mat, na.rm = TRUE)
} else {
mat = t(scale(t(mat)))
}
} else if("column" %in% scale) {
if(any(is.na(mat))) {
mat = t((t(mat) - colMeans(mat, na.rm = TRUE))/colSds(mat, na.rm = TRUE))
} else {
mat = scale(mat)
}
}
ht_param = list(matrix = mat)
if(!identical(scale, "none") && !identical(breaks, NA)) {
warning_wrap("It not suggested to both set `scale` and `breaks`. It makes the function confused.")
}
# if color is a color mapping function
if(is.function(color)) {
ht_param$col = color
if(!identical(breaks, NA)) {
warning_wrap("`breaks` is ignored when `color` is set as a color mapping function.")
}
} else {
if(identical(breaks, NA)) {
n_col = length(color)
if(identical(scale, "row") || identical(scale, "column")) {
lim = max(abs(mat), na.rm = TRUE)
ht_param$col = colorRamp2(seq(-lim, lim, length.out = n_col), color)
} else {
ht_param$col = colorRamp2(seq(min(mat, na.rm = TRUE), max(mat, na.rm = TRUE), length.out = n_col), color)
}
} else {
if(length(breaks) == length(color) + 1) {
ht_param$col = local({
breaks = breaks
color = color
fun = function(x) {
n = length(color)
df = data.frame(start = c(-Inf, breaks[seq_len(n)], breaks[n+1]),
end = c(breaks[1], breaks[1+seq_len(n)], Inf))
# tell which interval x is in
ind = numeric(length(x))
for(i in seq_along(x)) {
ind[i] = which(df$start <= x[i] & df$end > x[i])
}
ind = ind - 1
ind[ind < 1] = 1
ind[ind > n] = n
color[ind]
}
attr(fun, "breaks") = breaks
fun
})
} else if(length(breaks) == length(color)) {
ht_param$col = colorRamp2(breaks, color)
} else {
n_col = length(color)
ht_param$col = colorRamp2(seq(min(breaks), max(breaks), length.out = n_col), color)
warning_wrap("`breaks` does not have the same length as `color`. The colors are interpolated from the minimal to the maximal of `breaks`.")
}
}
}
if(!identical(filename, NA)) {
warning_wrap("argument `filename` is not supported in pheatmap -> Heatmap translation, skip it.")
}
if(!identical(width, NA)) {
warning_wrap("argument `width` is not supported in pheatmap -> Heatmap translation, skip it.")
}
if(!identical(height, NA)) {
warning_wrap("argument `height` is not supported in pheatmap -> Heatmap translation, skip it.")
}
if(!identical(silent, FALSE)) {
warning_wrap("argument `silent` is not supported in pheatmap -> Heatmap translation, skip it.")
}
ht_param$rect_gp = gpar(col = border_color)
if(nrow(mat) > 1000 || ncol(mat) > 1000) {
if(!is.na(border_color)) {
warning_wrap("border color is set for the matrix with large numbers of rows or columns. You might only be able to see the border colors in the plot. Set `border_color = NA` to get rid of it.")
}
}
if(!identical(cellwidth, NA)) {
ht_param$width = ncol(mat)*unit(cellwidth, "pt")
}
if(!identical(cellheight, NA)) {
ht_param$height = nrow(mat)*unit(cellheight, "pt")
}
if(identical(clustering_distance_rows, "correlation")) clustering_distance_rows = "pearson"
if(identical(clustering_distance_cols, "correlation")) clustering_distance_cols = "pearson"
ht_param$cluster_rows = cluster_rows
ht_param$cluster_columns = cluster_cols
ht_param$clustering_distance_rows = clustering_distance_rows
ht_param$clustering_distance_columns = clustering_distance_cols
ht_param$clustering_method_rows = clustering_method
ht_param$clustering_method_columns = clustering_method
if(!is.na(cutree_rows)) {
if(inherits(cluster_rows, c("logical", "hclust", "dendrogram"))) {
ht_param$row_split = cutree_rows
ht_param$row_gap = unit(4, "bigpts")
ht_param["row_title"] = list(NULL)
}
}
if(!is.na(cutree_cols)) {
if(inherits(cluster_cols, c("logical", "hclust", "dendrogram"))) {
ht_param$column_split = cutree_cols
ht_param$column_gap = unit(4, "bigpts")
ht_param["column_title"] = list(NULL)
}
}
ht_param$row_dend_width = unit(treeheight_row, "pt")
ht_param$column_dend_height = unit(treeheight_col, "pt")
ht_param$show_heatmap_legend = legend
if(identical(scale, "row") || identical(scale, "column")) {
if(identical(legend_breaks, NA)) {
lim = quantile(abs(mat), 0.975, na.rm = TRUE)
le = pretty(c(-lim, lim), n = 3)
if(length(le) == 7 && le[1] == -3) {
le = c(-3, -1.5, 0, 1.5, 3)
} else if(! 0 %in% le) {
le = c(le[1], le[1]/2, 0, le[length(le)]/2, le[length(le)])
}
legend_breaks = le
}
}
if(!identical(legend_breaks, NA)) {
heatmap_legend_param$at = legend_breaks
}
if(!identical(legend_labels, NA)) {
heatmap_legend_param$labels = legend_labels
}
ht_param$heatmap_legend_param = heatmap_legend_param
if(identical(annotation_colors, NA)) {
annotation_colors = list()
}
if(!identical(annotation_col, NA)) {
acn = rownames(annotation_col)
mcn = colnames(mat)
if(!is.null(acn)) {
if(acn[1] %in% mcn) {
if(length(union(acn, mcn)) == length(mcn)) {
if(!identical(acn, mcn)) {
warning_wrap("Column annotation has different order from matrix columns. Adjust the column annotation based on column names of the matrix.")
}
annotation_col = annotation_col[mcn, , drop = FALSE]
}
}
}
for(nm in colnames(annotation_col)) {
if(nm %in% names(annotation_colors)) {
if(is.null(names(annotation_colors[[nm]])) && is.numeric(annotation_col[, nm])) {
foo_x = annotation_col[, nm]
foo_n_col = length(annotation_colors[[nm]])
annotation_colors[[nm]] = colorRamp2(seq(min(foo_x), max(foo_x), length.out = foo_n_col), annotation_colors[[nm]])
}
}
}
ht_param$top_annotation = HeatmapAnnotation(df = annotation_col[, ncol(annotation_col):1, drop = FALSE],
col = annotation_colors, show_legend = annotation_legend,
show_annotation_name = annotation_names_col, gp = gpar(col = border_color),
annotation_name_gp = gpar(fontsize = 10, fontface = "bold"),
simple_anno_size = unit(10, "bigpts"), gap = unit(2, "bigpts"))
}
if(!identical(annotation_row, NA)) {
arn = rownames(annotation_row)
mrn = rownames(mat)
if(!is.null(arn)) {
if(arn[1] %in% mrn) {
if(length(union(arn, mrn)) == length(mrn)) {
if(!identical(arn, mrn)) {
warning_wrap("Row annotation has different order from matrix rows. Adjust the row annotation based on row names of the matrix.")
}
annotation_row = annotation_row[mrn, , drop = FALSE]
}
}
}
for(nm in colnames(annotation_row)) {
if(nm %in% names(annotation_colors)) {
if(is.null(names(annotation_colors[[nm]])) && is.numeric(annotation_row[, nm])) {
foo_x = annotation_row[, nm]
foo_n_col = length(annotation_colors[[nm]])
annotation_colors[[nm]] = colorRamp2(seq(min(foo_x), max(foo_x), length.out = foo_n_col), annotation_colors[[nm]])
}
}
}
ht_param$left_annotation = rowAnnotation(df = annotation_row[, ncol(annotation_row):1, drop = FALSE],
col = annotation_colors, show_legend = annotation_legend,
show_annotation_name = annotation_names_row, gp = gpar(col = border_color),
annotation_name_gp = gpar(fontsize = 10, fontface = "bold"),
simple_anno_size = unit(10, "bigpts"), gap = unit(2, "bigpts"))
}
if(!identical(annotation, NA)) {
warning_wrap("argument `annotation` is not supported in pheatmap -> Heatmap translation, skip it.")
}
if(identical(drop_levels, FALSE)) {
warning_wrap("argument `drop_levels` is enfored to be TRUE, skip it.")
}
ht_param$show_row_names = show_rownames
ht_param$show_column_names = show_colnames
ht_param$row_names_gp = gpar(fontsize = fontsize_row, fontfamily = fontfamily_row, fontface = fontface_row)
ht_param$column_names_gp = gpar(fontsize = fontsize_col, fontfamily = fontfamily_col, fontface = fontface_col)
angle_col = match.arg(angle_col)[1]
angle_col = switch(angle_col,
"0" = 0,
"45" = 45,
"90" = 90,
"270" = 90,
"315" = -45)
ht_param$column_names_rot = angle_col
if(angle_col == 0) {
ht_param$column_names_centered = TRUE
}
if(is.logical(display_numbers)) {
if(display_numbers) {
ht_param$layer_fun = local({
number_format = number_format
number_color = number_color
fontsize_number = fontsize_number
mat = mat
function(j, i, x, y, w, h, fill) {
grid.text(sprintf(number_format, pindex(mat, i, j)), x = x, y = y, gp = gpar(col = number_color, fontsize = fontsize_number))
}
})
}
} else if(is.matrix(display_numbers)) {
if(!identical(dim(display_numbers), dim(mat))) {
stop_wrap("dimension of `display_numbers` should be the same as the input matrix.")
}
ht_param$layer_fun = local({
number_color = number_color
fontsize_number = fontsize_number
mat = display_numbers
function(j, i, x, y, w, h, fill) {
grid.text(pindex(mat, i, j), x = x, y = y, gp = gpar(col = number_color, fontsize = fontsize_number))
}
})
}
if(!is.null(labels_row)) {
ht_param$row_labels = labels_row
}
if(!is.null(labels_col)) {
ht_param$column_labels = labels_col
}
if(!is.null(gaps_row)) {
if(inherits(cluster_rows, c("hclust", "dendrogram"))) {
stop_wrap("`gaps_row` should not be set when `cluster_rows` is set as a clustering object.")
}
if(identical(cluster_rows, TRUE)) {
stop_wrap("`gaps_row` should not be set when `cluster_rows` is set to TRUE.")
}
slices = diff(c(0, gaps_row, nrow(mat)))
ht_param$row_split = rep(seq_along(slices), times = slices)
ht_param$row_gap = unit(4, "bigpts")
ht_param["row_title"] = list(NULL)
}
if(!is.null(gaps_col)) {
if(inherits(cluster_cols, c("hclust", "dendrogram"))) {
stop_wrap("`gaps_col` should not be set when `cluster_cols` is set as a clustering object.")
}
if(identical(cluster_cols, TRUE)) {
stop_wrap("`gaps_col` should not be set when `cluster_cols` is set to TRUE.")
}
slices = diff(c(0, gaps_col, ncol(mat)))
ht_param$column_split = rep(seq_along(slices), times = slices)
ht_param$column_gap = unit(4, "bigpts")
ht_param["column_title"] = list(NULL)
}
if(!identical(clustering_callback, NA)) {
if(!identical(ht_param$cluster_rows, FALSE)) {
row_hclust = hclust(get_dist(mat, ht_param$clustering_distance_rows), ht_param$clustering_method_rows)
row_hclust = clustering_callback(row_hclust, ...)
ht_param$cluster_rows = row_hclust
}
if(!identical(ht_param$cluster_columns, FALSE)) {
column_hclust = hclust(get_dist(t(mat), ht_param$clustering_distance_columns), ht_param$clustering_method_columns)
column_hclust = clustering_callback(column_hclust, ...)
ht_param$cluster_columns = column_hclust
}
}
### default from pheatmap
ht_param$name = name
ht_param$row_dend_reorder = FALSE
ht_param$column_dend_reorder = FALSE
if(!identical(main, NA)) {
ht_param$column_title = main
ht_param$column_title_gp = gpar(fontface = "bold", fontsize = 1.3*fontsize)
}
ht_param = c(ht_param, list(...))
ht = do.call(Heatmap, ht_param)
attr(ht, "translate_from") = "pheatmap"
if(run_draw) {
draw(ht)
} else {
ht
}
}
# == title
# Compare heatmaps between pheatmap::pheatmap() and ComplexHeatmap::pheatmap()
#
# == param
# -... The same set of arguments passed to ``pheatmap::pheatmap`` and ``ComplexHeatmap::pheatmap``.
#
# == details
# The function plots two heatmaps, one by ``pheatmap::pheatmap`` and one by ``ComplexHeatmap::pheatmap``.
# Users can see the difference between the two implementations.
#
# == example
# mat = matrix(rnorm(100), 10)
# compare_pheatmap(mat)
compare_pheatmap = function(...) {
if(!requireNamespace("pheatmap")) {
stop_wrap("pheatmap package should be installed.")
}
p1 = pheatmap::pheatmap(..., silent = TRUE)$gtable
p2 = grid.grabExpr(draw(pheatmap(...)))
grid.newpage()
pushViewport(viewport(x = 0, width = 0.5, y = 0, height = unit(1, "npc") - unit(1, "cm"), just = c("left", "bottom")))
grid.draw(p1)
popViewport()
pushViewport(viewport(x = 0, width = 0.5, y = 1, height = unit(1, "cm"), just = c("left", "top")))
grid.text("pheatmap::pheatmap()")
popViewport()
pushViewport(viewport(x = 0.5, width = 0.5, y = 0, height = unit(1, "npc") - unit(1, "cm"), just = c("left", "bottom")))
grid.draw(p2)
popViewport()
pushViewport(viewport(x = 0.5, width = 0.5, y = 1, height = unit(1, "cm"), just = c("left", "top")))
grid.text("ComplexHeatmap::pheatmap()")
popViewport()
}
|