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\name{newSeqExpressionSet}
\alias{newSeqExpressionSet}
\title{
Function to create a new \code{\linkS4class{SeqExpressionSet}} object.
}
\description{
User-level function to create new objects of the class \code{\linkS4class{SeqExpressionSet}}.
}
\usage{
newSeqExpressionSet(counts,
normalizedCounts = matrix(data=NA, nrow=nrow(counts), ncol=ncol(counts), dimnames=dimnames(counts)),
offset = matrix(data=0, nrow=nrow(counts), ncol=ncol(counts), dimnames=dimnames(counts)),
phenoData = annotatedDataFrameFrom(counts, FALSE),
featureData = annotatedDataFrameFrom(counts, TRUE),
...)
}
\arguments{
\item{counts}{
A matrix containing the counts for an RNA-Seq experiment. One column for each lane and one row for each gene.
}
\item{normalizedCounts}{
A matrix with the same dimensions of \code{counts} with the normalized counts.
}
\item{offset}{
A matrix with the same dimensions of \code{counts} defining the offset (usually useful for normalization purposes). See the package vignette for a discussion on the offset.
}
\item{phenoData}{
A data.frame or \code{\link{AnnotatedDataFrame}} with sample information, such as biological condition, library preparation protocol, flow-cell,...
}
\item{featureData}{
A data.frame or \code{\link{AnnotatedDataFrame}} with feature information, such as gene length, GC-content, ...
}
\item{\dots}{
Other arguments will be passed to the constructor inherited from \code{\linkS4class{eSet}}.
}
}
\value{
An object of class \code{\linkS4class{SeqExpressionSet}}.
}
\author{
Davide Risso
}
\seealso{
\code{\linkS4class{SeqExpressionSet}}
}
\examples{
counts <- matrix(data=0, nrow=100, ncol=4)
for(i in 1:4) {
counts[, i] <- rpois(100, lambda=50)
}
cond <- c(rep("A", 2), rep("B", 2))
counts <- newSeqExpressionSet(counts, phenoData=data.frame(conditions=cond))
}
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