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\name{normalize.quantiles.target}
\alias{normalize.quantiles.use.target}
\alias{normalize.quantiles.determine.target}
\title{Quantile Normalization using a specified target distribution vector}
\description{
Using a normalization based upon quantiles, these function
normalizes the columns of a matrix based upon a specified
normalization distribution
}
\usage{
normalize.quantiles.use.target(x,target,copy=TRUE,subset=NULL)
normalize.quantiles.determine.target(x,target.length=NULL,subset=NULL)
}
\arguments{
\item{x}{A matrix of intensities where each column corresponds to a
chip and each row is a probe.}
\item{copy}{Make a copy of matrix before normalizing. Usually safer to
work with a copy}
\item{target}{A vector containing datapoints from the distribution to
be normalized to}
\item{target.length}{number of datapoints to return in target
distribution vector. If \code{NULL} then this will be taken to be
equal to the number of rows in the matrix.}
\item{subset}{A logical variable indexing whether corresponding row should be used in reference distribution determination}
}
\details{This method is based upon the concept of a quantile-quantile
plot extended to n dimensions. No special allowances are made for
outliers. If you make use of quantile normalization either through
\code{\link[affy]{rma}} or \code{\link[affy]{expresso}}
please cite Bolstad et al, Bioinformatics (2003).
These functions will handle missing data (ie NA values), based on the
assumption that the data is missing at random.
}
\value{
From \code{normalize.quantiles.use.target} a normalized \code{matrix}.
}
\references{
Bolstad, B (2001) \emph{Probe Level Quantile Normalization of High Density
Oligonucleotide Array Data}. Unpublished manuscript
\url{http://bmbolstad.com/stuff/qnorm.pdf}
Bolstad, B. M., Irizarry R. A., Astrand, M, and Speed, T. P. (2003)
\emph{A Comparison of Normalization Methods for High Density
Oligonucleotide Array Data Based on Bias and Variance.}
Bioinformatics 19(2) ,pp 185-193. \url{http://bmbolstad.com/misc/normalize/normalize.html}
}
\author{Ben Bolstad, \email{bmb@bmbolstad.com}}
\seealso{\code{\link{normalize.quantiles}}}
\keyword{manip}
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