1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
|
% Generated by roxygen2: do not edit by hand
% Please edit documentation in R/splitAltExps.R
\name{splitAltExps}
\alias{splitAltExps}
\title{Split off alternative features}
\usage{
splitAltExps(x, f, ref = NULL)
}
\arguments{
\item{x}{A \linkS4class{SingleCellExperiment} object.}
\item{f}{A character vector or factor of length equal to \code{nrow(x)}, specifying the feature type of each row.}
\item{ref}{String indicating which level of \code{f} should be treated as the main set.}
}
\value{
A SingleCellExperiment where each row corresponds to a feature in the main set.
Each other feature type is stored as an alternative Experiment, accessible by \code{\link{altExp}}.
\code{ref} is used as the \code{\link{mainExpName}}.
}
\description{
Split a \linkS4class{SingleCellExperiment} based on the feature type, creating alternative Experiments to hold features that are not in the majority set.
}
\details{
This function provides a convenient way to create a SingleCellExperiment with alternative Experiments.
For example, a SingleCellExperiment with rows corresponding to all features can be quickly split into endogenous genes (main)
and other alternative features like spike-in transcripts and antibody tags.
By default, the most frequent level of \code{f} is treated as the \code{ref} if the latter is not specified.
}
\examples{
example(SingleCellExperiment, echo=FALSE)
feat.type <- sample(c("endog", "ERCC", "CITE"), nrow(sce),
replace=TRUE, p=c(0.8, 0.1, 0.1))
sce2 <- splitAltExps(sce, feat.type)
sce2
}
\seealso{
\code{\link{altExp}}, to access and manipulate the alternative Experiment fields.
\code{\link{unsplitAltExps}}, to reverse the splitting.
}
\author{
Aaron Lun
}
|
