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#!/usr/bin/env perl
# Copyright © 2011, Battelle National Biodefense Institute (BNBI);
# all rights reserved. Authored by: Brian Ondov, Nicholas Bergman, and
# Adam Phillippy
#
# See the LICENSE.txt file included with this software for license information.
use strict;
BEGIN
{
use File::Basename;
use Cwd 'abs_path';
use lib dirname(abs_path($0)) . "/../lib";
use KronaTools;
}
setOption('out', 'taxonomy.krona.html');
setOption('name', 'Root');
my @options =
qw(
out
name
include
combine
queryCol
taxCol
scoreCol
magCol
depth
noRank
hueBad
hueGood
url
postUrl
taxonomy
);
getKronaOptions(@options);
if
(
@ARGV < 1
)
{
printUsage
(
'Creates a Krona chart based on taxonomy IDs and, optionally, magnitudes
and scores. Taxonomy IDs corresponding to a rank of "no rank" in the database
will be assigned to their parents to make the hierarchy less cluttered (e.g.
"Cellular organisms" will be assigned to "root").',
'taxonomy',
'Tab-delimited file with taxonomy IDs and (optionally) query IDs,
magnitudes and scores. By default, query IDs, taxonomy IDs and scores will be
taken from columns 1, 2 and 3, respectively (see -q, -t, -s, and -m). Lines
beginning with "#" will be ignored.',
1,
1,
\@options
);
exit 0;
}
if ( optionsConflict('queryCol', 'taxCol', 'magCol', 'scoreCol') )
{
ktWarn('Query column already in use; not reading query IDs.');
setOption('queryCol', undef);
}
if ( optionsConflict('scoreCol', 'taxCol', 'magCol') )
{
ktWarn('Score column already in use; not reading scores.');
setOption('scoreCol', undef);
}
if ( optionsConflict('magCol', 'taxCol') )
{
ktWarn('Magnitude column already in use; not reading magnitudes.');
setOption('magCol', undef);
}
my $tree = newTree();
print "Loading taxonomy...\n";
loadTaxonomy();
my $set = 0;
my @datasetNames;
my $useScore = 1; # is score column present?
my $eVal; # is score e-value (inferred by negative scores)?
my $useMag = getOption('magCol'); # using magnitude values?
foreach my $input (@ARGV)
{
my ($file, $magFile, $name) = parseDataset($input);
my %magnitudes;
if ( ! getOption('combine') )
{
push @datasetNames, $name;
}
print "Importing $file...\n";
# load magnitudes
if ( defined $magFile )
{
if ( getOption('queryCol') )
{
print " Loading magnitudes from $magFile...\n";
loadMagnitudes($magFile, \%magnitudes);
$useMag = 1;
}
else
{
ktWarn("Query column not defined; not reading magnitudes from \"$magFile\".");
}
}
open IN, "<$file" or ktDie("Couldn't open \"$file\".");
while ( <IN> )
{
if ( /^#/ )
{
next;
}
chomp;
my @fields = split /\t/;
my $queryID;
my $taxID = $fields[getOption('taxCol') - 1];
my $magnitude;
my $score;
if ( getOption('queryCol') )
{
$queryID = $fields[getOption('queryCol') - 1];
}
if ( getOption('scoreCol') )
{
$score = $fields[getOption('scoreCol') - 1];
}
if ( defined $queryID && defined $magnitudes{$queryID} )
{
$magnitude = $magnitudes{$queryID};
}
elsif ( defined getOption('magCol') )
{
$magnitude = $fields[getOption('magCol') - 1];
}
if ( $taxID == -2 )
{
$taxID = 1;
}
elsif ( $taxID == -1 )
{
if ( getOption('include') )
{
$taxID = 0;
}
else
{
next;
}
}
if ( ! defined $score )
{
# all lines must have score to be used
$useScore = 0;
}
if ( $score < 0 )
{
# score is probably e-value; flip colors
$eVal = 1;
}
addByTaxID($tree, $set, $taxID, $queryID, $magnitude, $score);
}
if ( ! getOption('combine') )
{
$set++;
}
close IN;
}
my @attributeNames =
(
'magnitude',
'magnitudeUnassigned',
'count',
'unassigned',
'taxon',
'rank',
);
my @attributeDisplayNames =
(
$useMag ? 'Magnitude' : (getOption('queryCol') ? undef : 'Count'),
$useMag ? 'Unassigned magnitude' : (getOption('queryCol') ? undef : 'Unassigned'),
getOption('queryCol') ? 'Count' : undef,
getOption('queryCol') ? 'Unassigned' : undef,
'Taxon',
'Rank',
);
my @scoreArgs;
if ( $useScore )
{
push @attributeNames, 'score';
push @attributeDisplayNames, 'Avg. score';
@scoreArgs =
(
$eVal ? getOption('hueGood') : getOption('hueBad'),
$eVal ? getOption('hueBad') : getOption('hueGood')
)
}
writeTree
(
$tree,
\@attributeNames,
\@attributeDisplayNames,
\@datasetNames,
@scoreArgs
);
sub optionsConflict
{
my ($option, @others) = @_;
if ( getOption($option) )
{
foreach my $other ( @others )
{
if ( getOption($option) == getOption($other) )
{
return 1;
}
}
}
return 0;
}
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