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/*
Ray -- Parallel genome assemblies for parallel DNA sequencing
Copyright (C) 2010, 2011, 2012, 2013 Sébastien Boisvert
http://DeNovoAssembler.SourceForge.Net/
This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, version 3 of the License.
This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
GNU General Public License for more details.
You have received a copy of the GNU General Public License
along with this program (gpl-3.0.txt).
see <http://www.gnu.org/licenses/>
*/
#include "FusionWorker.h"
#include <iostream>
using namespace std;
bool FusionWorker::isDone(){
return m_isDone;
}
/**
* work method.
*
* \author Sébastien Boisvert
*
* Code reviews
*
* 2011-09-02 -- Code review by Élénie Godzaridis (found bug with worker states)
*
*/
void FusionWorker::work(){
/*
used tags:
TODO: does the code pay attention when the coverage indicates a repeated k-mer ? repeats slow things down...
RAY_MPI_TAG_ASK_VERTEX_PATHS_SIZE
RAY_MPI_TAG_ASK_VERTEX_PATH
RAY_MPI_TAG_GET_PATH_LENGTH
*/
if(m_isDone)
return;
if(m_position < (int) m_path->size()){
/* get the number of paths */
if(!m_requestedNumberOfPaths){
/*
if(m_position % 1000 == 0){
cout<<"FusionWorker "<<m_workerIdentifier<<" position: ["<<m_position<<"/"<<m_path->size()<<endl;
}
*/
Kmer kmer;
m_path->at(m_position,&kmer);
if(m_reverseStrand)
kmer=kmer.complementVertex(m_parameters->getWordSize(),m_parameters->getColorSpaceMode());
Rank destination=kmer.vertexRank(m_parameters->getSize(),
m_parameters->getWordSize(),m_parameters->getColorSpaceMode());
int elementsPerQuery=m_virtualCommunicator->getElementsPerQuery(RAY_MPI_TAG_ASK_VERTEX_PATHS_SIZE);
MessageUnit*message=(MessageUnit*)m_outboxAllocator->allocate(elementsPerQuery);
int outputPosition=0;
kmer.pack(message,&outputPosition);
Message aMessage(message,elementsPerQuery,destination,
RAY_MPI_TAG_ASK_VERTEX_PATHS_SIZE,m_parameters->getRank());
m_virtualCommunicator->pushMessage(m_workerIdentifier,&aMessage);
m_requestedNumberOfPaths=true;
m_receivedNumberOfPaths=false;
if(m_parameters->hasOption("-debug-fusions2")){
cout<<"worker "<<m_workerIdentifier<<" send RAY_MPI_TAG_ASK_VERTEX_PATHS_SIZE"<<endl;
}
/* receive the number of paths */
}else if(m_requestedNumberOfPaths && !m_receivedNumberOfPaths && m_virtualCommunicator->isMessageProcessed(m_workerIdentifier)){
vector<MessageUnit> response;
m_virtualCommunicator->getMessageResponseElements(m_workerIdentifier,&response);
m_numberOfPaths=response[0];
if(m_parameters->hasOption("-debug-fusions2"))
cout<<"worker "<<m_workerIdentifier<<" Got "<<m_numberOfPaths<<endl;
m_receivedNumberOfPaths=true;
m_pathIndex=0;
m_requestedPath=false;
/* 2^5 */
int maximumNumberOfPathsToProcess=32;
/* don't process repeated stuff */
if(m_numberOfPaths> maximumNumberOfPathsToProcess)
m_numberOfPaths=0;
}else if(m_receivedNumberOfPaths && m_pathIndex < m_numberOfPaths){
/* request a path */
if(!m_requestedPath){
Kmer kmer;
m_path->at(m_position,&kmer);
if(m_reverseStrand)
kmer=kmer.complementVertex(m_parameters->getWordSize(),m_parameters->getColorSpaceMode());
Rank destination=kmer.vertexRank(m_parameters->getSize(),
m_parameters->getWordSize(),m_parameters->getColorSpaceMode());
int elementsPerQuery=m_virtualCommunicator->getElementsPerQuery(RAY_MPI_TAG_ASK_VERTEX_PATH);
MessageUnit*message=(MessageUnit*)m_outboxAllocator->allocate(elementsPerQuery);
int outputPosition=0;
kmer.pack(message,&outputPosition);
message[outputPosition++]=m_pathIndex;
Message aMessage(message,elementsPerQuery,destination,
RAY_MPI_TAG_ASK_VERTEX_PATH,m_parameters->getRank());
m_virtualCommunicator->pushMessage(m_workerIdentifier,&aMessage);
if(m_parameters->hasOption("-debug-fusions2")){
cout<<"worker "<<m_workerIdentifier<<" send RAY_MPI_TAG_ASK_VERTEX_PATH "<<m_pathIndex<<endl;
}
m_requestedPath=true;
m_receivedPath=false;
/* receive the path */
}else if(!m_receivedPath && m_virtualCommunicator->isMessageProcessed(m_workerIdentifier)){
vector<MessageUnit> response;
m_virtualCommunicator->getMessageResponseElements(m_workerIdentifier,&response);
int bufferPosition=0;
/* skip the k-mer because we don't need it */
bufferPosition+=KMER_U64_ARRAY_SIZE;
PathHandle otherPathIdentifier=response[bufferPosition++];
//int progression=response[bufferPosition++];
if(m_parameters->hasOption("-debug-fusions2"))
cout<<"worker "<<m_workerIdentifier<<" receive RAY_MPI_TAG_ASK_VERTEX_PATH_REPLY"<<endl;
if(otherPathIdentifier != m_identifier){
m_hits[otherPathIdentifier]++;
}
m_receivedPath=true;
m_pathIndex++;
m_requestedPath=false;
}
/* received all paths, can do the next one */
}else if(m_receivedNumberOfPaths && m_pathIndex == m_numberOfPaths){
m_position++;
m_requestedNumberOfPaths=false;
m_receivedNumberOfPaths=false;
if(m_parameters->hasOption("-debug-fusions2"))
cout<<"worker "<<m_workerIdentifier<<" Next position is "<<m_position<<endl;
}
/* gather hit information */
}else if(!m_gatheredHits){
if(!m_initializedGathering){
for(map<PathHandle,int>::iterator i=m_hits.begin();i!=m_hits.end();i++){
m_hitNames.push_back(i->first);
}
m_initializedGathering=true;
m_hitIterator=0;
m_requestedHitLength=false;
}else if(m_hitIterator < (int) m_hitNames.size()){
/* ask the hit length */
if(!m_requestedHitLength){
MessageUnit*message=(MessageUnit*)m_outboxAllocator->allocate(1);
PathHandle hitName=m_hitNames[m_hitIterator];
Rank destination=getRankFromPathUniqueId(hitName);
message[0]=hitName.getValue();
Message aMessage(message,1,destination,
RAY_MPI_TAG_GET_PATH_LENGTH,m_parameters->getRank());
m_virtualCommunicator->pushMessage(m_workerIdentifier,&aMessage);
m_requestedHitLength=true;
/* receive the hit length */
}else if(m_virtualCommunicator->isMessageProcessed(m_workerIdentifier)){
vector<MessageUnit> response;
m_virtualCommunicator->getMessageResponseElements(m_workerIdentifier,&response);
int length=response[0];
if(m_parameters->hasOption("-debug-fusions2"))
cout<<"received length, value= "<<length<<endl;
m_hitLengths.push_back(length);
m_hitIterator++;
m_requestedHitLength=false;
}
}else{
m_gatheredHits=true;
}
}else{
/* at this point, we have:
* m_hits
* m_hitNames
* m_hitLengths
*/
#ifdef CONFIG_ASSERT
assert(m_hits.size()==m_hitLengths.size());
assert(m_hitLengths.size()==m_hitNames.size());
assert(m_hitIterator == (int)m_hitLengths.size());
#endif
if(m_parameters->hasOption("-debug-fusions")){
cout<<"FusionWorker worker "<<m_workerIdentifier<<" path "<<m_identifier<<" strand= "<<m_reverseStrand<<" is Done, analyzed "<<m_position<<" position length is "<<m_path->size()<<endl;
cout<<"FusionWorker worker "<<m_hits.size()<<" hits "<<endl;
}
for(int i=0;i<(int)m_hitNames.size();i++){
PathHandle hit=m_hitNames[i];
int hitLength=m_hitLengths[i];
int selfLength=m_path->size();
int matches=m_hits[hit];
#ifdef CONFIG_ASSERT
assert(hit != m_identifier);
#endif
double ratio=(matches+0.0)/selfLength;
if(m_parameters->hasOption("-debug-fusions")){
cout<<"FusionWorker path "<<hit<<" matches= "<<matches<<" length= "<<hitLength<<endl;
}
if(ratio < 0.7)
continue;
/*
* We want to make sure that no sequence path is lost.
* They should be merged since it is not totally included.
*/
int biologicalObjectsWithoutMatch=selfLength-matches;
int maximumAllowedLost=1024;
if(biologicalObjectsWithoutMatch>maximumAllowedLost)
continue;
/* the other is longer anyway */
if(hitLength > selfLength){
m_eliminated=true;
}
/* the longer is "greater" but of equal length */
if(hitLength == selfLength && hit > m_identifier){
m_eliminated=true;
}
}
m_isDone=true;
}
}
WorkerHandle FusionWorker::getWorkerIdentifier(){
return m_workerIdentifier;
}
/**
* This class takes a path, and try to find another path that contains it.
*/
void FusionWorker::constructor(WorkerHandle number,GraphPath*path,PathHandle identifier,bool reverseStrand,
VirtualCommunicator*virtualCommunicator,Parameters*parameters,RingAllocator*outboxAllocator,
MessageTag RAY_MPI_TAG_ASK_VERTEX_PATH,
MessageTag RAY_MPI_TAG_ASK_VERTEX_PATHS_SIZE,
MessageTag RAY_MPI_TAG_GET_PATH_LENGTH
){
this->RAY_MPI_TAG_GET_PATH_LENGTH=RAY_MPI_TAG_GET_PATH_LENGTH;
this->RAY_MPI_TAG_ASK_VERTEX_PATHS_SIZE=RAY_MPI_TAG_ASK_VERTEX_PATHS_SIZE;
this->RAY_MPI_TAG_ASK_VERTEX_PATH=RAY_MPI_TAG_ASK_VERTEX_PATH;
m_virtualCommunicator=virtualCommunicator;
m_workerIdentifier=number;
m_initializedGathering=false;
m_gatheredHits=false;
m_isDone=false;
m_identifier=identifier;
m_reverseStrand=reverseStrand;
m_path=path;
m_eliminated=false;
m_outboxAllocator=outboxAllocator;
m_parameters=parameters;
m_position=0;
m_requestedNumberOfPaths=false;
if(m_parameters->hasOption("-debug-fusions")){
cout<<"Spawned worker number "<<number<<endl;
cout<<" path "<<m_identifier<<" reverse "<<m_reverseStrand<<" length "<<m_path->size()<<endl;
}
}
bool FusionWorker::isPathEliminated(){
return m_eliminated;
}
PathHandle FusionWorker::getPathIdentifier(){
return m_identifier;
}
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