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/*
Ray
Copyright (C) 2010, 2011, 2012 Sébastien Boisvert
http://DeNovoAssembler.SourceForge.Net/
This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, version 3 of the License.
This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
GNU General Public License for more details.
You have received a copy of the GNU General Public License
along with this program (gpl-3.0.txt).
see <http://www.gnu.org/licenses/>
*/
#include "BubbleTool.h"
#include <code/Mock/common_functions.h>
#include <assert.h>
#include <map>
#include <set>
#include <iostream>
using namespace std;
void BubbleTool::printStuff(Kmer root,vector<vector<Kmer> >*trees,
map<Kmer,int>*coverages){
int m_wordSize=m_parameters->getWordSize();
cout<<"Trees="<<trees->size()<<endl;
cout<<"root="<<root.idToWord(m_wordSize,m_parameters->getColorSpaceMode())<<endl;
cout<<"digraph{"<<endl;
map<Kmer,set<Kmer> > printedEdges;
for(map<Kmer ,int>::iterator i=coverages->begin();i!=coverages->end();i++){
Kmer kmer=i->first;
cout<<kmer.idToWord(m_wordSize,m_parameters->getColorSpaceMode())<<" [label=\""<<kmer.idToWord(m_wordSize,m_parameters->getColorSpaceMode())<<" "<<i->second<<"\"]"<<endl;
}
for(int j=0;j<(int)trees->size();j++){
for(int i=0;i<(int)trees->at(j).size();i+=2){
Kmer a=trees->at(j).at(i+0);
#ifdef CONFIG_ASSERT
assert(i+1<(int)trees->at(j).size());
#endif
Kmer b=trees->at(j).at(i+1);
if(printedEdges.count(a)>0 && printedEdges[a].count(b)>0){
continue;
}
cout<<a.idToWord(m_wordSize,m_parameters->getColorSpaceMode())<<" -> "<<b.idToWord(m_wordSize,m_parameters->getColorSpaceMode())<<endl;
printedEdges[a].insert(b);
}
}
cout<<"}"<<endl;
}
/**
*
*/
bool BubbleTool::isGenuineBubble(Kmer root,vector<vector<Kmer > >*trees,
map<Kmer ,int>*coverages,int repeatCoverage){
#ifdef NO_BUBBLES
return false;
#endif
if((*coverages)[root]>= repeatCoverage){
return false;
}
int m_wordSize=m_parameters->getWordSize();
#ifdef CONFIG_ASSERT
for(int i=0;i<(int)trees->size();i++){
for(int j=0;j<(int)trees->at(i).size();j+=2){
Kmer a=trees->at(i).at(j+0);
Kmer b=trees->at(i).at(j+1);
string as=a.idToWord(m_wordSize,m_parameters->getColorSpaceMode());
string bs=b.idToWord(m_wordSize,m_parameters->getColorSpaceMode());
assert(as.substr(1,m_wordSize-1)==bs.substr(0,m_wordSize-1));
}
}
#endif
if(m_parameters->debugBubbles()){
printStuff(root,trees,coverages);
}
if(trees->size()!=2){
return false;// we don'T support that right now ! triploid stuff are awesome.
}
// given the word size
// check that they join.
//
// substitution SNP is d=0
// del is 1, 2, or 3
map<Kmer ,int> coveringNumber;
Kmer target;
bool foundTarget=false;
for(int j=0;j<(int)trees->size();j++){
for(int i=0;i<(int)trees->at(j).size();i+=2){
Kmer a=trees->at(j).at(i+1);
#ifdef CONFIG_ASSERT
if(coverages->count(a)==0){
cout<<a.idToWord(m_parameters->getWordSize(),m_parameters->getColorSpaceMode())<<" has no coverage."<<endl;
}
assert(coverages->count(a)>0);
#endif
coveringNumber[a]++;
if(!foundTarget && coveringNumber[a]==2){
foundTarget=true;
target=a;
break;
}
}
}
if(!foundTarget){
if(m_parameters->debugBubbles()){
cout<<"Target not found."<<endl;
}
return false;
}
if((*coverages)[target]>= repeatCoverage){
return false;
}
#ifdef CONFIG_ASSERT
assert(coverages->count(root)>0);
assert(coverages->count(target)>0);
#endif
#ifdef CONFIG_ASSERT
int rootCoverage=(*coverages)[root];
int targetCoverage=(*coverages)[target];
assert(rootCoverage>0);
assert(targetCoverage>0);
#endif
vector<map<Kmer ,Kmer > > parents;
for(int j=0;j<(int)trees->size();j++){
map<Kmer ,Kmer > aVector;
parents.push_back(aVector);
for(int i=0;i<(int)trees->at(j).size();i+=2){
Kmer a=trees->at(j).at(i+0);
Kmer b=trees->at(j).at(i+1);
parents[j][b]=a;
}
}
vector<vector<int> > observedValues;
/*
*
* BUBBLE is below
*
* * ---- * -------* --------*
* \ /
* ---- * --------* ------ *
*
*/
// accumulate observed values
// and stop when encountering
for(int j=0;j<(int)trees->size();j++){
vector<int> aVector;
observedValues.push_back(aVector);
set<Kmer > visited;
Kmer startingPoint=trees->at(j).at(0);
Kmer current=target;
while(current!=startingPoint){
if(visited.count(current)>0){
return false;
}
visited.insert(current);
Kmer theParent=parents[j][current];
int coverageValue=(*coverages)[theParent];
observedValues[j].push_back(coverageValue);
current=theParent;
}
}
if(m_parameters->debugBubbles()){
cout<<"O1="<<observedValues[0].size()<<" O2="<<observedValues[1].size()<<endl;
}
int sum1=0;
for(int i=0;i<(int)observedValues[0].size();i++){
sum1+=observedValues[0][i];
}
if(m_parameters->debugBubbles()){
cout<<"O1Values= ";
for(int i=0;i<(int)observedValues[0].size();i++){
cout<<observedValues[0][i]<<" ";
}
cout<<endl;
}
int sum2=0;
for(int i=0;i<(int)observedValues[1].size();i++){
sum2+=observedValues[1][i];
}
if(m_parameters->debugBubbles()){
cout<<"O2Values= ";
for(int i=0;i<(int)observedValues[1].size();i++){
cout<<observedValues[1][i]<<" ";
}
cout<<endl;
}
if((int)observedValues[0].size()<2*m_parameters->getWordSize()
&& (int)observedValues[1].size()<2*m_parameters->getWordSize()){
if(sum1>sum2){
m_choice=trees->at(0).at(1);
}else if(sum2>sum1){
m_choice=trees->at(1).at(1);
// this will not happen often
}else if(sum1==sum2){
// take the shortest, if any
if(observedValues[0].size()<observedValues[1].size()){
m_choice=trees->at(0).at(1);
}else if(observedValues[1].size()<observedValues[0].size()){
m_choice=trees->at(1).at(1);
// same length and same sum, won't happen very often anyway
}else{
m_choice=trees->at(0).at(1);
}
}
if(m_parameters->debugBubbles()){
cout<<"This is a genuine bubble"<<endl;
cout<<"root="<<root.idToWord(m_wordSize,m_parameters->getColorSpaceMode())<<" target="<<target.idToWord(m_wordSize,m_parameters->getColorSpaceMode())<<endl;
}
return true;
}
if(m_parameters->debugBubbles()){
cout<<"False at last"<<endl;
}
return false;
}
Kmer BubbleTool::getTraversalStartingPoint(){
return m_choice;
}
void BubbleTool::constructor(Parameters*parameters){
m_parameters=parameters;
}
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