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/*
Ray -- Parallel genome assemblies for parallel DNA sequencing
Copyright (C) 2010, 2011, 2012, 2013 Sébastien Boisvert
http://DeNovoAssembler.SourceForge.Net/
This program is free software: you can redistribute it and/or modify
it under the terms of the GNU General Public License as published by
the Free Software Foundation, version 3 of the License.
This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
GNU General Public License for more details.
You have received a copy of the GNU General Public License
along with this program (gpl-3.0.txt).
see <http://www.gnu.org/licenses/>
*/
#include "SeedingData.h"
#include <code/Mock/constants.h>
#include <code/SeedingData/SeedWorker.h>
#include <RayPlatform/communication/Message.h>
#include <RayPlatform/communication/VirtualCommunicator.h>
#include <RayPlatform/communication/mpi_tags.h>
#include <RayPlatform/core/OperatingSystem.h>
#include <algorithm>
#include <fstream>
#include <sstream>
#include <assert.h>
__CreatePlugin(SeedingData);
__CreateSlaveModeAdapter(SeedingData,RAY_SLAVE_MODE_START_SEEDING);
__CreateSlaveModeAdapter(SeedingData,RAY_SLAVE_MODE_SEND_SEED_LENGTHS);
/*
* TODO: port this with the VirtualProcessor framework.
*/
void SeedingData::call_RAY_SLAVE_MODE_START_SEEDING(){
if(!m_initiatedIterator){
m_last=time(NULL);
m_SEEDING_i=0;
m_activeWorkerIterator=m_activeWorkers.begin();
m_splayTreeIterator.constructor(m_subgraph,m_wordSize,m_parameters);
m_initiatedIterator=true;
m_maximumAliveWorkers=32768;
#ifdef CONFIG_ASSERT
m_splayTreeIterator.hasNext();
#endif
m_virtualCommunicator->resetCounters();
}
if(!m_checkedCheckpoint){
if(m_parameters->hasCheckpoint("SimpleSeeds")){
cout<<"Rank "<<m_parameters->getRank()<<": checkpoint SimpleSeeds exists, not computing seeds."<<endl;
(*m_mode)=RAY_SLAVE_MODE_DO_NOTHING;
Message aMessage(NULL,0,MASTER_RANK,RAY_MPI_TAG_SEEDING_IS_OVER,getRank());
m_outbox->push_back(&aMessage);
loadCheckpoint();
return;
}
m_checkedCheckpoint=true;
}
m_virtualCommunicator->processInbox(&m_activeWorkersToRestore);
if(!m_virtualCommunicator->isReady()){
return;
}
// flush all mode is necessary to empty buffers and
// restart things from scratch..
// 1. iterate on active workers
if(m_activeWorkerIterator!=m_activeWorkers.end()){
WorkerHandle workerId=*m_activeWorkerIterator;
#ifdef CONFIG_ASSERT
assert(m_aliveWorkers.count(workerId)>0);
assert(!m_aliveWorkers[workerId].isDone());
#endif
m_virtualCommunicator->resetLocalPushedMessageStatus();
//force the worker to work until he finishes or pushes something on the stack
while(!m_aliveWorkers[workerId].isDone()&&!m_virtualCommunicator->getLocalPushedMessageStatus()){
m_aliveWorkers[workerId].work();
}
if(m_virtualCommunicator->getLocalPushedMessageStatus()){
m_waitingWorkers.push_back(workerId);
}
if(m_aliveWorkers[workerId].isDone()){
m_workersDone.push_back(workerId);
GraphPath*seed=m_aliveWorkers[workerId].getSeed();
int minimalSeedLength = 100;
if(m_parameters->hasConfigurationOption("-minimum-seed-length", 1)) {
minimalSeedLength = m_parameters->getConfigurationInteger("-minimum-seed-length", 0);
}
int nucleotides=getNumberOfNucleotides(seed->size(),m_wordSize);
if(seed->size() > 0 && m_debugSeeds){
cout<<"Raw seed length: "<<nucleotides<<" nucleotides"<<endl;
}
#ifdef CONFIG_ASSERT
assert(nucleotides==0 || nucleotides>=m_wordSize);
#endif
SeedWorker*worker=&(m_aliveWorkers[workerId]);
bool isSmall = nucleotides < 4 * m_parameters->getWordSize();
if(isSmall && worker->isHeadADeadEnd() && worker->isTailADeadEnd()){
m_skippedObjectsWithTwoDeadEnds++;
}else if(isSmall && worker->isHeadADeadEnd()){
m_skippedObjectsWithDeadEndForHead++;
}else if(isSmall && worker->isTailADeadEnd()){
m_skippedObjectsWithDeadEndForTail++;
}else if(isSmall && worker->isBubbleWeakComponent()){
m_skippedObjectsWithBubbleWeakComponent++;
// only consider the long ones.
}else if(nucleotides >= minimalSeedLength){
#ifdef SHOW_DISCOVERIES
printf("Rank %i discovered a seed with %i vertices\n",m_rank,(int)seed.size());
#endif
#ifdef CONFIG_ASSERT
assert(seed->size()>0);
#endif
Kmer firstVertex;
seed->at(0,&firstVertex);
Kmer lastVertex;
seed->at(seed->size()-1,&lastVertex);
Kmer firstReverse=m_parameters->_complementVertex(&lastVertex);
int minimumNucleotidesForVerbosity=1024;
bool verbose=nucleotides>=minimumNucleotidesForVerbosity;
if(m_debugSeeds){
verbose=true;
}
bool ignoreSeeds = m_parameters->hasOption("-ignore-seeds");
if(firstVertex<firstReverse && !ignoreSeeds){
if(verbose){
printf("Rank %i stored a seed with %i vertices\n",m_rank,(int)seed->size());
}
if(m_parameters->showMemoryUsage() && verbose){
showMemoryUsage(m_rank);
}
GraphPath*theSeed=seed;
theSeed->computePeakCoverage();
CoverageDepth peakCoverage=theSeed->getPeakCoverage();
if(verbose)
cout<<"Got a seed, peak coverage: "<<peakCoverage;
/* ignore the seed if it has too much coverage. */
if(peakCoverage >= m_minimumSeedCoverageDepth
&& peakCoverage <= m_parameters->getMaximumSeedCoverage()){
if(verbose)
cout<<", adding seed."<<endl;
m_SEEDING_seeds.push_back(*theSeed);
m_eligiblePaths++;
}else{
if(verbose)
cout<<", ignoring seed."<<endl;
m_skippedNotEnoughCoverage++;
}
}else{
m_skippedNotMine++;
}
}else{
m_skippedTooShort++;
}
}
m_activeWorkerIterator++;
}else{
updateStates();
// add one worker to active workers
// reason is that those already in the pool don't communicate anymore --
// as for they need responses.
if(!m_virtualCommunicator->getGlobalPushedMessageStatus()&&m_activeWorkers.empty()){
// there is at least one worker to start
// AND
// the number of alive workers is below the maximum
if(m_SEEDING_i<m_subgraph->size()&&(int)m_aliveWorkers.size()<m_maximumAliveWorkers){
if(m_SEEDING_i % 100000 ==0){
printf("Rank %i is creating seeds [%i/%i]\n",getRank(),(int)m_SEEDING_i+1,(int)m_subgraph->size());
if(m_parameters->showMemoryUsage()){
showMemoryUsage(m_rank);
}
}
#ifdef CONFIG_ASSERT
if(m_SEEDING_i==0){
assert(m_completedJobs==0&&m_activeWorkers.size()==0&&m_aliveWorkers.size()==0);
}
#endif
m_splayTreeIterator.next();
Kmer vertexKey=*(m_splayTreeIterator.getKey());
m_aliveWorkers[m_SEEDING_i].constructor(&vertexKey,m_parameters,m_outboxAllocator,m_virtualCommunicator,m_SEEDING_i,
RAY_MPI_TAG_GET_VERTEX_EDGES_COMPACT,
RAY_MPI_TAG_REQUEST_VERTEX_COVERAGE
);
if(m_debugSeeds)
m_aliveWorkers[m_SEEDING_i].enableDebugMode();
m_activeWorkers.insert(m_SEEDING_i);
int population=m_aliveWorkers.size();
if(population>m_maximumWorkers){
m_maximumWorkers=population;
}
m_SEEDING_i++;
// skip the reverse complement as we don't really need it anyway.
}else{
m_virtualCommunicator->forceFlush();
}
}
// brace yourself for the next round
m_activeWorkerIterator=m_activeWorkers.begin();
}
#ifdef CONFIG_ASSERT
assert((int)m_aliveWorkers.size()<=m_maximumAliveWorkers);
#endif
if((int)m_subgraph->size()==m_completedJobs){
printf("Rank %i has %i seeds\n",m_rank,(int)m_SEEDING_seeds.size());
printf("Rank %i is creating seeds [%i/%i] (completed)\n",getRank(),(int)m_SEEDING_i,(int)m_subgraph->size());
printf("Rank %i: peak number of workers: %i, maximum: %i\n",m_rank,m_maximumWorkers,m_maximumAliveWorkers);
m_virtualCommunicator->printStatistics();
cout<<"Rank "<<m_rank<<" runtime statistics for seeding algorithm: "<<endl;
cout<<"Rank "<<m_rank<<" Skipped paths because of dead end for head: "<<m_skippedObjectsWithDeadEndForHead<<endl;
cout<<"Rank "<<m_rank<<" Skipped paths because of dead end for tail: "<<m_skippedObjectsWithDeadEndForTail<<endl;
cout<<"Rank "<<m_rank<<" Skipped paths because of two dead ends: "<<m_skippedObjectsWithTwoDeadEnds<<endl;
cout<<"Rank "<<m_rank<<" Skipped paths because of bubble weak component: "<<m_skippedObjectsWithBubbleWeakComponent<<endl;
cout<<"Rank "<<m_rank<<" Skipped paths because of short length: "<<m_skippedTooShort<<endl;
cout<<"Rank "<<m_rank<<" Skipped paths because of bad ownership: "<<m_skippedNotMine<<endl;
cout<<"Rank "<<m_rank<<" Skipped paths because of low coverage: "<<m_skippedNotEnoughCoverage<<endl;
cout<<"Rank "<<m_rank<<" Eligible paths: "<<m_eligiblePaths<<endl;
#ifdef CONFIG_ASSERT
assert(m_eligiblePaths==(int)m_SEEDING_seeds.size());
#endif
(*m_mode)=RAY_SLAVE_MODE_DO_NOTHING;
Message aMessage(NULL,0,MASTER_RANK,RAY_MPI_TAG_SEEDING_IS_OVER,getRank());
m_outbox->push_back(&aMessage);
if(m_parameters->showMemoryUsage()){
showMemoryUsage(m_rank);
}
#ifdef CONFIG_ASSERT
assert(m_aliveWorkers.size()==0);
assert(m_activeWorkers.size()==0);
#endif
// sort the seeds by length
std::sort(m_SEEDING_seeds.begin(),
m_SEEDING_seeds.end(), comparePaths);
/**************************************************************
* Write down the SimpleSeeds checkpoint now.
**********************************************************************/
writeCheckpoints();
}
}
void SeedingData::constructor(SeedExtender*seedExtender,int rank,int size,StaticVector*outbox,RingAllocator*outboxAllocator,
int*mode,
Parameters*parameters,GridTable*subgraph,StaticVector*inbox,
VirtualCommunicator*vc){
m_skippedObjectsWithDeadEndForHead=0;
m_skippedObjectsWithDeadEndForTail=0;
m_skippedObjectsWithTwoDeadEnds=0;
m_skippedObjectsWithBubbleWeakComponent=0;
m_skippedTooShort=0;
m_skippedNotMine=0;
m_skippedNotEnoughCoverage=0;
m_eligiblePaths=0;
m_checkedCheckpoint=false;
m_virtualCommunicator=vc;
m_seedExtender=seedExtender;
m_size=size;
m_rank=rank;
m_outbox=outbox;
m_inbox=inbox;
m_completedJobs=0;
m_maximumWorkers=0;
m_flushAllMode=false;
m_outboxAllocator=outboxAllocator;
m_mode=mode;
m_parameters=parameters;
m_wordSize=m_parameters->getWordSize();
#ifdef CONFIG_ASSERT
assert(m_wordSize>=15&&m_wordSize<=CONFIG_MAXKMERLENGTH);
#endif
m_subgraph=subgraph;
m_initiatedIterator=false;
m_minimumSeedCoverageDepth=0;
if(m_parameters->hasConfigurationOption("-use-minimum-seed-coverage",1)){
m_minimumSeedCoverageDepth=m_parameters->getConfigurationInteger("-use-minimum-seed-coverage",0);
cout<<"[SeedingData] will use "<<m_minimumSeedCoverageDepth<<" for the minimum seed coverage"<<endl;
}
m_debugSeeds=false;
if(m_parameters->hasConfigurationOption("-debug-seeds",0))
m_debugSeeds=true;
}
int SeedingData::getRank(){
return m_rank;
}
int SeedingData::getSize(){
return m_size;
}
void SeedingData::updateStates(){
// erase completed jobs
for(int i=0;i<(int)m_workersDone.size();i++){
WorkerHandle workerId=m_workersDone[i];
#ifdef CONFIG_ASSERT
assert(m_activeWorkers.count(workerId)>0);
assert(m_aliveWorkers.count(workerId)>0);
#endif
m_activeWorkers.erase(workerId);
m_aliveWorkers.erase(workerId);
m_completedJobs++;
}
m_workersDone.clear();
for(int i=0;i<(int)m_waitingWorkers.size();i++){
WorkerHandle workerId=m_waitingWorkers[i];
#ifdef CONFIG_ASSERT
assert(m_activeWorkers.count(workerId)>0);
#endif
m_activeWorkers.erase(workerId);
}
m_waitingWorkers.clear();
for(int i=0;i<(int)m_activeWorkersToRestore.size();i++){
WorkerHandle workerId=m_activeWorkersToRestore[i];
m_activeWorkers.insert(workerId);
}
m_activeWorkersToRestore.clear();
m_virtualCommunicator->resetGlobalPushedMessageStatus();
}
void SeedingData::call_RAY_SLAVE_MODE_SEND_SEED_LENGTHS(){
if(!m_initialized){
m_virtualCommunicator->resetCounters();
m_initialized = true;
}
Message aMessage(NULL,0,MASTER_RANK,
RAY_MPI_TAG_IS_DONE_SENDING_SEED_LENGTHS,getRank());
m_outbox->push_back(&aMessage);
(*m_mode)=RAY_SLAVE_MODE_DO_NOTHING;
}
void SeedingData::loadCheckpoint(){
cout<<"Rank "<<m_parameters->getRank()<<" is reading checkpoint SimpleSeeds"<<endl;
ifstream f(m_parameters->getCheckpointFile("SimpleSeeds").c_str());
int n=0;
f.read((char*)&n,sizeof(int));
for(int i=0;i<n;i++){
GraphPath seed;
seed.setKmerLength(m_parameters->getWordSize());
int vertices=0;
f.read((char*)&vertices,sizeof(int));
for(int j=0;j<vertices;j++){
Kmer kmer;
kmer.read(&f);
seed.push_back(&kmer);
CoverageDepth coverageValue=0;
f.read((char*)&coverageValue,sizeof(CoverageDepth));
seed.addCoverageValue(coverageValue);
}
seed.computePeakCoverage();
m_SEEDING_seeds.push_back(seed);
}
cout<<"Rank "<<m_parameters->getRank()<<" loaded "<<n<<" seeds from checkpoint SimpleSeeds"<<endl;
f.close();
}
void SeedingData::writeCheckpoints(){
/* write the Seeds checkpoint */
if(m_parameters->writeCheckpoints() && !m_parameters->hasCheckpoint("SimpleSeeds")){
ofstream f(m_parameters->getCheckpointFile("SimpleSeeds").c_str());
ostringstream buffer;
cout<<"Rank "<<m_parameters->getRank()<<" is writing checkpoint SimpleSeeds"<<endl;
vector<GraphPath> * seeds = & m_SEEDING_seeds;
int count=(*seeds).size();
buffer.write((char*)&count, sizeof(int));
for(int i=0;i<(int)(*seeds).size();i++){
int length=(*seeds)[i].size();
buffer.write((char*)&length, sizeof(int));
for(int j=0;j<(int)(*seeds)[i].size();j++){
Kmer theKmer;
(*seeds)[i].at(j,&theKmer);
theKmer.write(&buffer);
CoverageDepth coverageValue=0;
coverageValue=(*seeds)[i].getCoverageAt(j);
buffer.write((char*)&coverageValue, sizeof(CoverageDepth));
flushFileOperationBuffer(false, &buffer, &f, CONFIG_FILE_IO_BUFFER_SIZE);
}
}
flushFileOperationBuffer(true, &buffer, &f, CONFIG_FILE_IO_BUFFER_SIZE);
f.close();
}
}
void SeedingData::registerPlugin(ComputeCore*core){
m_core=core;
PluginHandle plugin=core->allocatePluginHandle();
m_plugin=plugin;
core->setPluginName(plugin,"SeedingData");
core->setPluginDescription(plugin,"Computes unique paths in the graph");
core->setPluginAuthors(plugin,"Sébastien Boisvert");
core->setPluginLicense(plugin,"GNU General Public License version 3");
__ConfigureSlaveModeHandler(SeedingData, RAY_SLAVE_MODE_START_SEEDING);
__ConfigureSlaveModeHandler(SeedingData, RAY_SLAVE_MODE_SEND_SEED_LENGTHS);
RAY_MPI_TAG_SEND_SEED_LENGTHS_REPLY=core->allocateMessageTagHandle(plugin);
core->setMessageTagSymbol(plugin,RAY_MPI_TAG_SEND_SEED_LENGTHS_REPLY,"RAY_MPI_TAG_SEND_SEED_LENGTHS_REPLY");
__BindPlugin(SeedingData);
m_core->setObjectSymbol(m_plugin, &m_SEEDING_seeds,"/RayAssembler/ObjectStore/Seeds.ray");
}
void SeedingData::resolveSymbols(ComputeCore*core){
RAY_SLAVE_MODE_START_SEEDING=core->getSlaveModeFromSymbol(m_plugin,"RAY_SLAVE_MODE_START_SEEDING");
RAY_SLAVE_MODE_DO_NOTHING=core->getSlaveModeFromSymbol(m_plugin,"RAY_SLAVE_MODE_DO_NOTHING");
RAY_SLAVE_MODE_SEND_SEED_LENGTHS=core->getSlaveModeFromSymbol(m_plugin,"RAY_SLAVE_MODE_SEND_SEED_LENGTHS");
RAY_MPI_TAG_GET_VERTEX_EDGES_COMPACT=core->getMessageTagFromSymbol(m_plugin,"RAY_MPI_TAG_GET_VERTEX_EDGES_COMPACT");
RAY_MPI_TAG_REQUEST_VERTEX_COVERAGE=core->getMessageTagFromSymbol(m_plugin,"RAY_MPI_TAG_REQUEST_VERTEX_COVERAGE");
RAY_MPI_TAG_IS_DONE_SENDING_SEED_LENGTHS=core->getMessageTagFromSymbol(m_plugin,"RAY_MPI_TAG_IS_DONE_SENDING_SEED_LENGTHS");
RAY_MPI_TAG_SEEDING_IS_OVER=core->getMessageTagFromSymbol(m_plugin,"RAY_MPI_TAG_SEEDING_IS_OVER");
RAY_MPI_TAG_SEND_SEED_LENGTHS=core->getMessageTagFromSymbol(m_plugin,"RAY_MPI_TAG_SEND_SEED_LENGTHS");
RAY_MPI_TAG_SEND_SEED_LENGTHS_REPLY=core->getMessageTagFromSymbol(m_plugin,"RAY_MPI_TAG_SEND_SEED_LENGTHS_REPLY");
}
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