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#include "SuffixArrayFuns.h"
#include "PackedArray.h"
inline uint medianUint2(uint a, uint b)
{
// returns (a+b)/2
return a/2 + b/2 + (a%2 + b%2)/2;
};
uint compareSeqToGenome(Genome &mapGen, char** s2, uint S, uint N, uint L, uint iSA, bool dirR, bool& compRes)
{
/* compare s to g, find the maximum identity length
* s2[0] read sequence; s2[1] complementary sequence
* S position to start search from in s2[0],s2[1]
* dirR forward or reverse direction search on read sequence
*/
register int64 ii;
uint SAstr=mapGen.SA[iSA];
bool dirG = (SAstr>>mapGen.GstrandBit) == 0; //forward or reverse strand of the genome
SAstr &= mapGen.GstrandMask;
char *g=mapGen.G;
if (dirR && dirG) {//forward on read, forward on genome
char* s = s2[0] + S + L;
g += SAstr + L;
for (ii=0;(uint) ii < N-L; ii++)
{
if (s[ii]!=g[ii])
{
if (s[ii]>g[ii])
{
compRes=true;
return ii+L;
} else
{
compRes=false;
return ii+L;
};
};
};
// if (s[ii]>g[ii]) {compRes=true;} else {compRes=false;};
return N; //exact match
} else if (dirR && !dirG) {
char* s = s2[1] + S + L;
g += mapGen.nGenome-1-SAstr - L;
for (ii=0; (uint) ii < N-L; ii++)
{
if (s[ii]!=g[-ii])
{
if (s[ii]>g[-ii] || g[-ii]>3)
{
compRes=false;
return ii+L;
} else
{
compRes=true;
return ii+L;
};
};
};
return N;
} else if (!dirR && dirG) {
char* s = s2[1] + S - L;
g += SAstr + L;
for (ii=0; (uint) ii < N-L; ii++)
{
if (s[-ii]!=g[ii])
{
if (s[-ii]>g[ii]) {
compRes=true;
return ii+L;
} else
{
compRes=false;
return ii+L;
};
};
};
return N;
} else {//if (!dirR && !dirG)
char* s = s2[0] + S - L;
g += mapGen.nGenome-1-SAstr - L;
for (ii=0; (uint) ii < N-L; ii++)
{
if (s[-ii]!=g[-ii])
{
if (s[-ii]>g[-ii] || g[-ii]>3)
{
compRes=false;
return ii+L;
} else
{
compRes=true;
return ii+L;
};
};
};
return N;
};
};
uint findMultRange(Genome &mapGen, uint i3, uint L3, uint i1, uint L1, uint i1a, uint L1a, uint i1b, uint L1b, char** s, bool dirR, uint S)
{ // given SA index i3 and identity length L3, return the index of the farthest element with the same length, starting from i1,L1 or i1a,L1a, or i1b,L1b
bool compRes;
if (L1<L3) { //search between i1 and i3
L1b=L1; i1b=i1; i1a=i3;
}
else {
if (L1a<L1) {//search between i1a and i1b, else: search bewtween i1a and i1b
L1b=L1a; i1b=i1a; i1a=i1;
};
};
while ( (i1b+1<i1a)|(i1b>i1a+1) ) { //L1a is the target length, i1a...i1b is the initial range, i1c,L1c is the value in the middle
uint i1c=medianUint2(i1a,i1b);
//uint L1c=identityLength(&g[mapGen.SA[i3]+L1b],&g[mapGen.SA[i1c]+L1b],L3-L1b)+L1b;
uint L1c=compareSeqToGenome(mapGen,s,S,L3,L1b,i1c,dirR,compRes);
if (L1c==L3) {
i1a=i1c;
}
else { //L1c<L3, move i1c
i1b=i1c;L1b=L1c;
};
};
return i1a;
};
uint maxMappableLength(Genome &mapGen, char** s, uint S, uint N, uint i1, uint i2, bool dirR, uint& L, uint* indStartEnd)
{
/* find minimum mappable length of sequence s to the genome g with suffix array SA; length(s)=N; [i1 i2] is initial suffix array search bounds.
* returns number of mappings (1=unique);range indStartEnd; min mapped length = L
* binary search in SA space
*/
bool compRes;
uint L1,L2,i3,L3,L1a,L1b,L2a,L2b,i1a,i1b,i2a,i2b;
L1=compareSeqToGenome(mapGen,s,S,N,L,i1,dirR,compRes);
L2=compareSeqToGenome(mapGen,s,S,N,L,i2,dirR,compRes);
// L1=identityLength(&s[L],&g[mapGen.SA[i1]]);
// L2=identityLength(&s[L],&g[mapGen.SA[i2]]);
L= min(L1,L2);
L1a=L1;L1b=L1;i1a=i1;i1b=i1;
L2a=L2;L2b=L2;i2a=i2;i2b=i2; // track boundaries of best matching suffix array ranges
// suffix array
// ---------------------------
// i1, L1
//
// i3, L3
//
// i2, L2
// --------------------------
i3=i1;L3=L1; //in case i1+1>=i2 an not iteration of the loope below is ever made
while (i1+1<i2) {//main binary search loop
i3=medianUint2(i1,i2);
L3=compareSeqToGenome(mapGen,s,S,N,L,i3,dirR,compRes);
if (L3==N) break; //found exact match, exit the binary search
if (compRes) { //move 1 to 3
if (L3>L1) {
L1b=L1a; L1a=L1; i1b=i1a; i1a=i1;
// L1b, i1b - captures history of last time the max score shifted.
// L1a, i1a - tracks current shift.
};
i1=i3;L1=L3;
}
else {
if (L3>L2) { //move 2 to 3
L2b=L2a; L2a=L2; i2b=i2a; i2a=i2;
};
i2=i3;L2=L3;
};
L= min(L1,L2);
};
if (L3<N) {//choose longest alignment length between L1 and L2
if (L1>L2) {
i3=i1;L3=L1;
} else {
i3=i2;L3=L2;
};
};
// now i3,L3 is the "best" alignment, i.e. longest length
// find the range of SA indices in which the identiyLength is the same
i1=findMultRange(mapGen,i3,L3,i1,L1,i1a,L1a,i1b,L1b,s,dirR,S);
i2=findMultRange(mapGen,i3,L3,i2,L2,i2a,L2a,i2b,L2b,s,dirR,S);
L=L3; //output
indStartEnd[0]=i1;
indStartEnd[1]=i2;
return i2-i1+1;
};
int compareRefEnds (Genome &mapGen, uint64 SAstr, uint64 gInsert, bool strG, bool strR)
{
if ( strG)
{// + strand g
return strR ? (SAstr < gInsert ? 1:-1) : 1;
} else
{// - strand g
return strR ? -1 : ( gInsert==-1LLU ? -1 : ( SAstr < mapGen.nGenome-gInsert ? 1:-1) );
};
};
uint compareSeqToGenome1(Genome &mapGen, char** s2, uint S, uint N, uint L, uint iSA, bool dirR, uint64 gInsert, int & compRes)
{
/* compare s to g, find the maximum identity length
* s2[0] read sequence; s2[1] complementary sequence
* S position to start search from in s2[0],s2[1]
* dirR: strand of the s
* different treatment of 5 (spacer) in the sequence and genome
* 5 is allowed in the sequence
* 5 in the genome is < than 5 in the sequence
*/
//TODO no need for complementary sequence
register int64 ii;
uint SAstr=mapGen.SA[iSA];
bool dirG = (SAstr>>mapGen.GstrandBit) == 0; //forward or reverse strand of the genome
SAstr &= mapGen.GstrandMask;
char *g=mapGen.G;
if (dirG) {//forward on read, forward on genome
char* s = s2[0] + S + L;
g += SAstr + L;
for (ii=0;(uint) ii < N-L; ii++)
{
if (s[ii]!=g[ii])
{
if (s[ii]>g[ii])
{
compRes=1;
return ii+L;
} else
{
compRes=-1;
return ii+L;
};
} else if (s[ii]==GENOME_spacingChar)
{//this already implies the s[ii]==g[ii]
compRes=compareRefEnds (mapGen, SAstr, gInsert, dirG, dirR);
return ii+L;
};
};
// if (s[ii]>g[ii]) {compRes=true;} else {compRes=false;};
return N; //exact match
}
else
{
char* s = s2[1] + S + L;
g += mapGen.nGenome-1-SAstr - L;
for (ii=0; (uint) ii < N-L; ii++)
{
if (s[ii]!=g[-ii])
{
char s1=s[ii],g1=g[-ii];
if (s1<4) s1=3-s1;
if (g1<4) g1=3-g1;
if (s1>g1) {
compRes=1;
return ii+L;
} else
{
compRes=-1;
return ii+L;
};
break;
} else if (s[ii]==GENOME_spacingChar)
{//this already implies the s[ii]==g[ii]
compRes=compareRefEnds (mapGen, SAstr, gInsert, dirG, dirR);
return ii+L;
};
};
return N;
};
};
uint suffixArraySearch1(Genome &mapGen, char** s, uint S, uint N, uint64 gInsert, bool strR, uint i1, uint i2, uint L)
{
/* binary search in SA space
* s[0],s[1] - query sequence, complementary sequence
* S - start offset
* N - sequence length
* g - genome sequence
* gInsert - index where the sequence insertion happened
* SA - suffix array
* strR - strand of the query sequence
* i1,i2 = starting indices in SA
* L - starting length
* output: first SA index > searched string, i.e. g[SA[index-1]]<s<g[SA[index]]
*/
int compRes;
uint L1=compareSeqToGenome1(mapGen,s,S,N,L,i1,strR,gInsert,compRes);
if (compRes<0)
{// the sequence is smaller than the first index of the SA, cannot proceed
L=L1;
return 0;
};
uint L2=compareSeqToGenome1(mapGen, s,S,N,L,i2,strR,gInsert,compRes);
if (compRes>0)
{//the sequence is bigger than the last SA index, return a huge number
L=L2;
return -2llu;
};
L=min(L1,L2);
uint i3=i1,L3=L1; //in case i1+1>=i2 an not iteration of the loope below is ever made
while (i1+1<i2) {//main binary search loop
i3=medianUint2(i1,i2);
L3=compareSeqToGenome1(mapGen,s,S,N,L,i3,strR,gInsert,compRes);//cannot do this because these sj sequences contains spacers=5
if (L3==N) {//this should not really happen
L=N;
return i3;
// cerr << "Bug L3==N"<<endl;
// exit(-1); //found exact match of the whole read length, exit the binary search
};
if (compRes>0)
{ //move 1 to 3
i1=i3;L1=L3;
} else if (compRes<0)
{//move 2 to 3
i2=i3;L2=L3;
}
L= min(L1,L2);
};
return i2; //index at i2 is always bigger than the sequence
};
uint funCalcSAiFromSA(char* gSeq, PackedArray& gSA, Genome &mapGen, uint iSA, int L, int & iL4)
{
uint SAstr=gSA[iSA];
bool dirG = (SAstr>>mapGen.GstrandBit) == 0; //forward or reverse strand of the genome
SAstr &= mapGen.GstrandMask;
iL4=-1;
register uint saind=0;
if (dirG)
{
register uint128 g1=*( (uint128*) (gSeq+SAstr) );
for (int ii=0; ii<L; ii++)
{
register char g2=(char) g1;
if (g2>3)
{
iL4=ii;
saind <<= 2*(L-ii);
return saind;
};
saind=saind<<2;
saind+=g2;
g1=g1>>8;
};
return saind;
} else
{
register uint128 g1=*( (uint128*) (gSeq+mapGen.nGenome-SAstr-16) );
for (int ii=0; ii<L; ii++)
{
register char g2=(char) (g1>>(8*(15-ii)));
if (g2>3)
{
iL4=ii;
saind <<= 2*(L-ii);
return saind;
};
saind=saind<<2;
saind+=3-g2;
};
return saind;
};
};
int64 funCalcSAi(char *G, uint iL)
{
int64 ind1=0;
for (uint iL1=0;iL1<=iL;iL1++) {
uint g=(uint) G[iL1];
if (g>3) {
return -ind1;
} else {
ind1 <<= 2;
ind1 += g;
};
};
return ind1;
};
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