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#
# = bio/sequence/format.rb - various output format of the biological sequence
#
# Copyright:: Copyright (C) 2006-2008
# Toshiaki Katayama <k@bioruby.org>,
# Naohisa Goto <ng@bioruby.org>,
# Ryan Raaum <ryan@raaum.org>,
# Jan Aerts <jan.aerts@bbsrc.ac.uk>
# License:: The Ruby License
#
require 'erb'
require 'date'
module Bio
class Sequence
# = DESCRIPTION
# A Mixin[http://www.rubycentral.com/book/tut_modules.html]
# of methods used by Bio::Sequence#output to output sequences in
# common bioinformatic formats. These are not called in isolation.
#
# = USAGE
# # Given a Bio::Sequence object,
# puts s.output(:fasta)
# puts s.output(:genbank)
# puts s.output(:embl)
module Format
# Repository of generic (or both nucleotide and protein) sequence
# formatter classes
module Formatter
# Raw format generatar
autoload :Raw, 'bio/sequence/format_raw'
# Fasta format generater
autoload :Fasta, 'bio/db/fasta/format_fasta'
# NCBI-style Fasta format generatar
# (resemble to EMBOSS "ncbi" format)
autoload :Fasta_ncbi, 'bio/db/fasta/format_fasta'
# FASTQ "fastq-sanger" format generator
autoload :Fastq, 'bio/db/fastq/format_fastq'
# FASTQ "fastq-sanger" format generator
autoload :Fastq_sanger, 'bio/db/fastq/format_fastq'
# FASTQ "fastq-solexa" format generator
autoload :Fastq_solexa, 'bio/db/fastq/format_fastq'
# FASTQ "fastq-illumina" format generator
autoload :Fastq_illumina, 'bio/db/fastq/format_fastq'
# FastaNumericFormat format generator
autoload :Fasta_numeric, 'bio/db/fasta/format_qual'
# Qual format generator.
# Its format is the same as Fasta_numeric, but it would perform
# to convert quality score or generates scores from error probability.
autoload :Qual, 'bio/db/fasta/format_qual'
end #module Formatter
# Repository of nucleotide sequence formatter classes
module NucFormatter
# GenBank format generater
# Note that the name is 'Genbank' and NOT 'GenBank'
autoload :Genbank, 'bio/db/genbank/format_genbank'
# EMBL format generater
# Note that the name is 'Embl' and NOT 'EMBL'
autoload :Embl, 'bio/db/embl/format_embl'
end #module NucFormatter
# Repository of protein sequence formatter classes
module AminoFormatter
# currently no formats available
end #module AminoFormatter
# Formatter base class.
# Any formatter class should inherit this class.
class FormatterBase
# Returns a formatterd string of the given sequence
# ---
# *Arguments*:
# * (required) _sequence_: Bio::Sequence object
# * (optional) _options_: a Hash object
# *Returns*:: String object
def self.output(sequence, options = {})
self.new(sequence, options).output
end
# register new Erb template
def self.erb_template(str)
erb = ERB.new(str)
erb.def_method(self, 'output')
true
end
private_class_method :erb_template
# generates output data
# ---
# *Returns*:: String object
def output
raise NotImplementedError, 'should be implemented in subclass'
end
# creates a new formatter object for output
def initialize(sequence, options = {})
@sequence = sequence
@options = options
end
private
# any unknown methods are delegated to the sequence object
def method_missing(sym, *args, &block) #:nodoc:
begin
@sequence.__send__(sym, *args, &block)
rescue NoMethodError => evar
lineno = __LINE__ - 2
file = __FILE__
bt_here = [ "#{file}:#{lineno}:in \`__send__\'",
"#{file}:#{lineno}:in \`method_missing\'"
]
if bt_here == evar.backtrace[0, 2] then
bt = evar.backtrace[2..-1]
evar = evar.class.new("undefined method \`#{sym.to_s}\' for #{self.inspect}")
evar.set_backtrace(bt)
end
raise(evar)
end
end
end #class FormatterBase
# Using Bio::Sequence::Format, return a String with the Bio::Sequence
# object formatted in the given style.
#
# Formats currently implemented are: 'fasta', 'genbank', and 'embl'
#
# s = Bio::Sequence.new('atgc')
# puts s.output(:fasta) #=> "> \natgc\n"
#
# The style argument is given as a Ruby
# Symbol(http://www.ruby-doc.org/core/classes/Symbol.html)
# ---
# *Arguments*:
# * (required) _format_: :fasta, :genbank, *or* :embl
# *Returns*:: String object
def output(format = :fasta, options = {})
formatter_const = format.to_s.capitalize.intern
formatter_class = nil
get_formatter_repositories.each do |mod|
begin
formatter_class = mod.const_get(formatter_const)
rescue NameError
end
break if formatter_class
end
unless formatter_class then
raise "unknown format name #{format.inspect}"
end
formatter_class.output(self, options)
end
# Returns a list of available output formats for the sequence
# ---
# *Arguments*:
# *Returns*:: Array of Symbols
def list_output_formats
a = get_formatter_repositories.collect { |mod| mod.constants }
a.flatten!
a.collect! { |x| x.to_s.downcase.intern }
a
end
# The same as output(:fasta, :header=>definition, :width=>width)
# This method is intended to replace Bio::Sequence#to_fasta.
#
# s = Bio::Sequence.new('atgc')
# puts s.output_fasta #=> "> \natgc\n"
# ---
# *Arguments*:
# * (optional) _definition_: (String) definition line
# * (optional) _width_: (Integer) width (default 70)
# *Returns*:: String object
def output_fasta(definition = nil, width = 70)
output(:fasta, :header=> definition, :width => width)
end
private
# returns formatter repository modules
def get_formatter_repositories
if self.moltype == Bio::Sequence::NA then
[ NucFormatter, Formatter ]
elsif self.moltype == Bio::Sequence::AA then
[ AminoFormatter, Formatter ]
else
[ NucFormatter, AminoFormatter, Formatter ]
end
end
#---
# Not yet implemented :)
# Remove the nodoc command after implementation!
# ---
# *Returns*:: String object
#def format_gff #:nodoc:
# raise NotImplementedError
#end
#+++
# Formatting helper methods for INSD (NCBI, EMBL, DDBJ) feature table
module INSDFeatureHelper
private
# INTERNAL USE ONLY, YOU SHOULD NOT CALL THIS METHOD. (And in any
# case, it would be difficult to successfully call this method outside
# its expected context).
#
# Output the Genbank feature format string of the sequence.
# Used in Bio::Sequence#output.
# ---
# *Returns*:: String object
def format_features_genbank(features)
prefix = ' ' * 5
indent = prefix + ' ' * 16
fwidth = 79 - indent.length
format_features(features, prefix, indent, fwidth)
end
# INTERNAL USE ONLY, YOU SHOULD NOT CALL THIS METHOD. (And in any
# case, it would be difficult to successfully call this method outside
# its expected context).
#
# Output the EMBL feature format string of the sequence.
# Used in Bio::Sequence#output.
# ---
# *Returns*:: String object
def format_features_embl(features)
prefix = 'FT '
indent = prefix + ' ' * 16
fwidth = 80 - indent.length
format_features(features, prefix, indent, fwidth)
end
# format INSD featurs
def format_features(features, prefix, indent, width)
result = []
features.each do |feature|
result.push format_feature(feature, prefix, indent, width)
end
return result.join('')
end
# format an INSD feature
def format_feature(feature, prefix, indent, width)
result = prefix + sprintf("%-16s", feature.feature)
position = feature.position
#position = feature.locations.to_s
result << wrap_and_split_lines(position, width).join("\n" + indent)
result << "\n"
result << format_qualifiers(feature.qualifiers, indent, width)
return result
end
# format qualifiers
def format_qualifiers(qualifiers, indent, width)
qualifiers.collect do |qualifier|
q = qualifier.qualifier
v = qualifier.value.to_s
if v == true
lines = wrap_with_newline('/' + q, width)
elsif q == 'translation'
lines = fold("/#{q}=\"#{v}\"", width)
else
if v[/\D/] or q == 'chromosome'
#v.delete!("\x00-\x1f\x7f-\xff")
v.gsub!(/"/, '""')
v = '"' + v + '"'
end
lines = wrap_with_newline('/' + q + '=' + v, width)
end
lines.gsub!(/^/, indent)
lines
end.join
end
def fold(str, width)
str.gsub(Regexp.new("(.{1,#{width}})"), "\\1\n")
end
def fold_and_split_lines(str, width)
str.scan(Regexp.new(".{1,#{width}}"))
end
def wrap_and_split_lines(str, width)
result = []
lefts = str.chomp.split(/(?:\r\n|\r|\n)/)
lefts.each do |left|
left.rstrip!
while left and left.length > width
line = nil
width.downto(1) do |i|
if left[i..i] == ' ' or /[\,\;]/ =~ left[(i-1)..(i-1)] then
line = left[0..(i-1)].sub(/ +\z/, '')
left = left[i..-1].sub(/\A +/, '')
break
end
end
if line.nil? then
line = left[0..(width-1)]
left = left[width..-1]
end
result << line
left = nil if left.to_s.empty?
end
result << left if left
end
return result
end
def wrap_with_newline(str, width)
result = wrap_and_split_lines(str, width)
result_string = result.join("\n")
result_string << "\n" unless result_string.empty?
return result_string
end
def wrap(str, width = 80, prefix = '')
actual_width = width - prefix.length
result = wrap_and_split_lines(str, actual_width)
result_string = result.join("\n#{prefix}")
result_string = prefix + result_string unless result_string.empty?
return result_string
end
#--
# internal use only
MonthStr = [ nil,
'JAN', 'FEB', 'MAR', 'APR', 'MAY', 'JUN',
'JUL', 'AUG', 'SEP', 'OCT', 'NOV', 'DEC'
].collect { |x| x.freeze }.freeze
#++
# formats a date from Date, DateTime, or Time object, or String.
def format_date(d)
begin
yy = d.year
mm = d.month
dd = d.day
rescue NoMethodError, NameError, ArgumentError, TypeError
return sprintf("%-11s", d)
end
sprintf("%02d-%-3s-%04d", dd, MonthStr[mm], yy)
end
# null date
def null_date
Date.new(0, 1, 1)
end
end #module INSDFeatureHelper
end #module Format
end #class Sequence
end #module Bio
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