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require_relative "segment_ends_path"
#
# Methods for the RGFA class, which allow to find and merge linear paths.
#
module RGFA::LinearPaths
require "set"
#
# Find a path without branches.
#
# The path must
# include +segment+ and excludes segments in +exclude+.
# Any segment used in the returned path will be added to +exclude+
#
# @param s [String|RGFA::Line::Segment] a segment name or instance
# @param exclude [Set<String>] a set of segment names to exclude from the path
# @return [Array<RGFA::SegmentEnd>]
#
def linear_path(s, exclude = Set.new)
s = s.to_sym
cs = connectivity(s)
segpath = RGFA::SegmentEndsPath.new()
[:B, :E].each_with_index do |et, i|
if cs[i] == 1
exclude << s
segpath.pop
segpath += traverse_linear_path(RGFA::SegmentEnd.new([s, et]), exclude)
end
end
return (segpath.size < 2) ? nil : segpath
end
# Find all unbranched paths in the graph.
#
# @return [Array<Array<RGFA::SegmentEnd>>]
def linear_paths
exclude = Set.new
retval = []
segnames = segment_names
progress_log_init(:linear_paths, "segments", segnames.size,
"Detect linear paths (#{segnames.size} segments)") if @progress
segnames.each do |sn|
progress_log(:linear_paths) if @progress
next if exclude.include?(sn)
retval << linear_path(sn, exclude)
end
progress_log_end(:linear_paths)
return retval.compact
end
# Merge a linear path, i.e. a path of segments without extra-branches
# @!macro [new] merge_lim
# Limitations: all containments und paths involving merged segments are
# deleted.
#
# @param segpath [Array<RGFA::SegmentEnd>] a linear path, such as that
# retrieved by {#linear_path}
# @!macro [new] merge_options
# @param options [Hash] optional keyword arguments
# @option options [String, :short, nil] :merged_name (nil)
# if nil, the merged_name is automatically computed; if :short,
# a name is computed starting with "merged1" and calling next until
# an available name is founf; if String, the name to use
# @option options [Boolean] :cut_counts (false)
# if true, total count in merged segment m, composed of segments
# s of set S is multiplied by the factor Sum(|s in S|)/|m|
#
# @return [RGFA] self
# @see #merge_linear_paths
def merge_linear_path(segpath, **options)
return if segpath.size < 2
segpath.map!{|se|se.to_segment_end}
if segpath[1..-2].any? {|sn,et| connectivity(sn) != [1,1]}
raise ArgumentError, "The specified path is not linear"
end
merged, first_reversed, last_reversed =
create_merged_segment(segpath, options)
self << merged
link_merged(merged.name, segpath.first.to_segment_end.invert_end_type,
first_reversed)
link_merged(merged.name, segpath.last, last_reversed)
segpath.each do |sn_et|
delete_segment(sn_et.segment)
progress_log(:merge_linear_paths, 0.05) if @progress
end
self
end
# Merge all linear paths in the graph, i.e.
# paths of segments without extra-branches
# @!macro merge_lim
# @!macro merge_options
#
# @return [RGFA] self
def merge_linear_paths(**options)
paths = linear_paths
psize = paths.flatten.size / 2
progress_log_init(:merge_linear_paths, "segments", psize,
"Merge #{paths.size} linear paths (#{psize} segments)") if @progress
paths.each do |path|
merge_linear_path(path, **options)
end
progress_log_end(:merge_linear_paths)
self
end
private
# Traverse the links, starting from the segment +from+ :E end if
# +traverse_from_E_end+ is true, or :B end otherwise.
#
# If any segment after +from+ is found whose name is included in +exclude+
# the traversing is interrupted. The +exclude+ set is updated, so that
# circular paths are avoided.
#
# *Arguments*:
# - +from+ -> first segment
# - +traverse_from_E_end+ -> if true, start from E end, otherwise from B end
# - +exclude+ -> Set of names of already visited segments
#
# *Side Effects*:
# - Any element added to the returned list is also added to +exclude+
#
# *Returns*:
# - An array of segment names of the unbranched path.
# If +from+ is not an element of an unbranched path then [].
# Otherwise the first (and possibly only) element is +from+.
# All elements in the index range 1..-2 are :internal.
def traverse_linear_path(segment_end, exclude)
list = RGFA::SegmentEndsPath.new()
current = segment_end
loop do
after = links_of(current)
before = links_of(current.to_segment_end.invert_end_type)
cs = connectivity_symbols(before.size, after.size)
if cs == [1,1] or list.empty?
list << current
exclude << current.name
l = after.first
current = l.other_end(current).invert_end_type
break if exclude.include?(current.name)
elsif cs[0] == 1
list << current
exclude << current.name
break
else
break
end
end
return segment_end.end_type == :B ? list.reverse : list
end
def sum_of_counts(segpath, multfactor = 1)
retval = {}
segs = segpath.map {|sn,et|segment!(sn)}
[:KC, :RC, :FC].each do |count_tag|
segs.each do |s|
if s.optional_fieldnames.include?(count_tag)
retval[count_tag] ||= 0
retval[count_tag] += s.get(count_tag)
end
end
if retval[count_tag]
retval[count_tag] = (retval[count_tag] * multfactor).to_i
end
end
return retval
end
def reverse_segment_name(name, separator)
name.to_s.split(separator).map do |part|
openp = part[0] == "("
part = part[1..-1] if openp
closep = part[-1] == ")"
part = part[0..-2] if closep
part = (part[-1] == "^") ? part[0..-2] : part+"^"
part += ")" if openp
part = "(#{part}" if closep
part
end.reverse.join(separator)
end
def reverse_pos_array(pos_array, lastpos)
return nil if pos_array.nil? or lastpos.nil?
pos_array.map {|pos| lastpos - pos + 1}.reverse
end
def add_segment_to_merged(merged, segment, reversed, cut, init, options)
s = (reversed ? segment.sequence.rc[cut..-1] : segment.sequence[cut..-1])
if init
merged.sequence = s
merged.name = (options[:merged_name].nil? ?
segment.name : options[:merged_name])
merged.LN = segment.LN
else
(segment.sequence == "*") ? (merged.sequence = "*")
: (merged.sequence += s)
if options[:merged_name].nil?
merged.name = "#{merged.name}_#{segment.name}"
end
if merged.LN
segment.LN ? merged.LN += (segment.LN - cut)
: merged.LN = nil
end
end
end
def create_merged_segment(segpath, options)
merged = segment!(segpath.first.first).clone
total_cut = 0
a = segpath.first
first_reversed = (a.end_type == :B)
last_reversed = nil
if options[:merged_name] == :short
forbidden = (segment_names + path_names)
options[:merged_name] = "merged1"
while forbidden.include?(options[:merged_name])
options[:merged_name] = options[:merged_name].next
end
end
add_segment_to_merged(merged, segment(a.segment), first_reversed, 0, true,
options)
progress_log(:merge_linear_paths, 0.95) if @progress
(segpath.size-1).times do |i|
b = segpath[i+1].to_segment_end.invert_end_type
l = link!(a, b)
if l.overlap == []
cut = 0
elsif l.overlap.all?{|op|[:M, :"="].include?(op.code)}
cut = l.overlap.map(&:len).inject(:+)
else
raise ArgumentError,
"Merging is only allowed if all operations are M/="
end
total_cut += cut
last_reversed = (b[1] == :E)
add_segment_to_merged(merged, segment(b.segment), last_reversed, cut,
false, options)
a = b.to_segment_end.invert_end_type
if @progress
progress_log(:merge_linear_paths, 0.95)
end
end
if merged.sequence != "*"
if merged.LN.nil?
merged.LN = merged.sequence.length
elsif @validate and merged.LN != merged.sequence.length
raise RGFA::Line::Segment::InconsistentLengthError,
"Computed sequence length #{merged.sequence.length} "+
"and computed LN #{merged.LN} differ"
end
end
if merged.LN.nil?
[:KC, :RC, :FC].each {|count_tag| merged.set(count_tag, nil)}
else
sum_of_counts(segpath, (options[:cut_counts] ?
merged.LN.to_f / (total_cut+merged.LN) : 1)).
each do |count_tag, count|
merged.set(count_tag, count)
end
end
return merged, first_reversed, last_reversed
end
def link_merged(merged_name, segment_end, reversed)
links_of(segment_end).each do |l|
l2 = l.clone
if l2.to == segment_end.first
l2.to = merged_name
if reversed
l2.to_orient = RGFA::OrientedSegment.invert(l2.to_orient)
end
else
l2.from = merged_name
if reversed
l2.from_orient = RGFA::OrientedSegment.invert(l2.from_orient)
end
end
self << l2
end
end
end
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