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#ifndef __LIBRARY_TYPE_DETECTOR__
#define __LIBRARY_TYPE_DETECTOR__
#include "spdlog/fmt/ostr.h"
#include "spdlog/fmt/fmt.h"
#include "spdlog/sinks/ostream_sink.h"
#include "spdlog/spdlog.h"
#include "LibraryFormat.hpp"
#include <atomic>
#include <mutex>
class LibraryTypeDetector {
public:
LibraryTypeDetector(ReadType type) : type_(type),
libTypeCounts_(std::vector<std::atomic<uint64_t>>(LibraryFormat::maxLibTypeID() + 1)) {}
LibraryTypeDetector(const LibraryTypeDetector& other) {
active_ = other.active_;
type_ = other.type_;
numSamplesNeeded_.store(other.numSamplesNeeded_.load());
libTypeCounts_ = std::vector<std::atomic<uint64_t>>(LibraryFormat::maxLibTypeID() + 1);
for (size_t i = 0; i < other.libTypeCounts_.size(); ++i) {
libTypeCounts_[i] = other.libTypeCounts_[i].load();
}
}
bool isActive() { return active_; }
bool canGuess() { return numSamplesNeeded_ <= 0; }
bool mostLikelyType(LibraryFormat& ifmt) {
bool ret{false};
if (mut_.try_lock()) {
if (active_) {
if (type_ == ReadType::SINGLE_END) {
uint64_t nf{0};
uint64_t nr{0};
for (size_t i = 0; i <= LibraryFormat::maxLibTypeID(); ++i) {
auto f = LibraryFormat::formatFromID(i);
auto c = libTypeCounts_[i].load();
nf += (f.strandedness == ReadStrandedness::S) ? c : 0;
nr += (f.strandedness == ReadStrandedness::A) ? c : 0;
}
double ratio = -1.0;
if (nf + nr > 0) {
ratio = static_cast<double>(nf) / (nf + nr);
}
ifmt.type = type_;
ifmt.orientation = ReadOrientation::NONE;
// If we have some issue computing this, leave as unstranded
if (ratio < 0.0) {
ifmt.strandedness = ReadStrandedness::U;
} else if (ratio < 0.3) {
// If we map to the forward strand < 30% of the time, we are antisesnse
ifmt.strandedness = ReadStrandedness::A;
} else if (ratio < 0.7) {
// Between 30% and 70% of the time, we are unstranded
ifmt.strandedness = ReadStrandedness::U;
} else {
// Greater than 70% of the time, we are sense
ifmt.strandedness = ReadStrandedness::S;
}
} else { // paired end
uint64_t nsf{0};
uint64_t nsr{0};
uint64_t ninward{0};
uint64_t noutward{0};
uint64_t nsame{0};
for (size_t i = 0; i <= LibraryFormat::maxLibTypeID(); ++i) {
auto f = LibraryFormat::formatFromID(i);
auto c = libTypeCounts_[i].load();
nsf += (f.strandedness == ReadStrandedness::S or
f.strandedness == ReadStrandedness::SA) ? c : 0;
nsr += (f.strandedness == ReadStrandedness::A or
f.strandedness == ReadStrandedness::AS) ? c : 0;
ninward += (f.orientation == ReadOrientation::TOWARD) ? c : 0;
noutward += (f.orientation == ReadOrientation::AWAY) ? c : 0;
nsame += (f.orientation == ReadOrientation::SAME) ? c : 0;
}
ifmt.type = type_;
if ((ninward + noutward + nsame > 0) and
(nsf + nsr > 0)){
auto numOrient = ninward + noutward + nsame;
double ratioIn = static_cast<double>(ninward) / numOrient;
double ratioOut = static_cast<double>(noutward) / numOrient;
double ratioSame = static_cast<double>(nsame) / numOrient;
ifmt.orientation = ReadOrientation::NONE;
bool same{false};
if (ratioIn >= ratioOut and ratioIn >= ratioSame) {
ifmt.orientation = ReadOrientation::TOWARD;
} else if (ratioOut >= ratioIn and ratioOut >= ratioSame) {
ifmt.orientation = ReadOrientation::AWAY;
} else {
ifmt.orientation = ReadOrientation::SAME;
same = true;
}
auto numStrand = nsf + nsr;
double ratioFW = static_cast<double>(nsf) / numStrand;
if (ratioFW < 0.3) {
ifmt.strandedness = (same) ? ReadStrandedness::A : ReadStrandedness::AS;
} else if (ratioFW < 0.7) {
ifmt.strandedness = ReadStrandedness::U;
} else {
ifmt.strandedness = (same) ? ReadStrandedness::S : ReadStrandedness::SA;
}
} else {
ifmt.orientation = ReadOrientation::TOWARD;
ifmt.strandedness = ReadStrandedness::U;
}
} // end paired-end
auto log = spdlog::get("jointLog");
log->info("Automatically detected most likely library type as {}", ifmt.toString());
active_ = false;
ret = true;
} // end if active_
mut_.unlock(); // release the lock
} // end try_lock()
return ret;
}
void addSample(LibraryFormat f) {
if (f.type == type_ and numSamplesNeeded_ >= 0) {
++libTypeCounts_[f.formatID()];
--numSamplesNeeded_;
}
}
private:
// set to false once we have guessed the type
bool active_{true};
std::mutex mut_;
// single or paired-end
ReadType type_;
// number of samples needed before we can guess a type
std::atomic<int64_t> numSamplesNeeded_{50000};
// the counts for each library type
std::vector<std::atomic<uint64_t>> libTypeCounts_;
};
#endif //__LIBRARY_TYPE_DETECTOR__
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