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\documentclass[a4paper,12pt]{scrartcl}
\usepackage[utf8]{inputenc}
\usepackage[table]{xcolor} % Farben
\usepackage{xspace} % platzhalter
\usepackage{tabularx}
\usepackage{array} % für Tabellenerstellung
\usepackage{booktabs} % für Tabellenerstellu
\usepackage{listings}
\newenvironment{packed_enum}{
\begin{enumerate}
\setlength{\itemsep}{1pt}
\setlength{\parskip}{0pt}
\setlength{\parsep}{0pt}
}{\end{enumerate}}
% Title Page
\title{Developer / User-Guide on new SeqAn IO}
\author{Hannes Hauswedell}
\usepackage{setspace}
\parindent0pt
\parskip1.5ex
\setstretch{1.1}
\newcommand{\todo}[1]{{\color{red}TODO:#1}\xspace}
\begin{document}
\section{General User and Developer Info}
\subsection{Some notes}
\begin{itemize}
\item \verb#https://trac.mi.fu-berlin.de/seqan/wiki/IoRevision# is partly
outdated, do not rely on it!
\item Currently all new IO-Code lives in SeqAn \emph{beside} the old code, you
do not need any \verb|#define|s to switch to the new code
\item Currently only FastA and FastQ are supported in the new code base
\item If you use only FastA and/or FastQ, you should definitely switch now,
since the new code is faster and more reliable
\item switching is fairly easy, you only need to construct a \verb#RecordReader#
object for reading and work on that (see below and dddoc for more details)
\item the new calls are \verb#readRecord()#, \verb#read2()# (read entire document),
\verb#writeRecord()# and \verb#write2()# (write entire document).
\end{itemize}
\subsection{Some examples}
\lstset
{
language=C++,
basicstyle=\color{black}\footnotesize\tt,
backgroundcolor=\color{white},
% numbers=left,
% numberstyle=\tiny,
% numbersep=5pt,
captionpos=b,
stringstyle=\ttfamily,
frame=single,
showspaces=false,
breaklines=true, % sets automatic line breaking
breakatwhitespace=true
% sets if automatic breaks should only happen at whitespace
}
Example code for reading a single record from a gzipped file (you will probably
want to loop over \verb#readRecord()#):
\begin{lstlisting}
char filename[] = "/path/to/file.fasta.gz";
gzFile file = gzopen(filename, "rb");
if (gzdirect(file))
{
// error handling
}
Stream<GZFile> stream(file);
RecordReader<Stream<GZFile>, SinglePass<> > reader(stream);
CharString meta;
DnaString seq;
res = readRecord(meta, seq, reader, Fasta());
if (res != 0)
{
// error handling
}
gzclose(file);
\end{lstlisting}
Example code for reading an entire file efficiently (using
memory-mapped strings as files and ConcatDirect-Strings as storage):
\begin{lstlisting}
typedef String<char, MMap<> > TMMapString;
TMMapString stream;
char filename[] = "/path/to/file.fasta";
int res = open(stream, filename, OPEN_RDONLY));
if (res != 0)
{
// error handling
}
RecordReader<TMMapString, DoublePass<Mapped> > reader(stream);
StringSet<CharString, Owner<ConcatDirect<> > > sequenceIds;
StringSet<DnaString, Owner<ConcatDirect<> > > sequences;
res = read2(sequenceIds, sequences, reader, Fasta());
if (res != 0)
{
// error handling
}
close(stream);
\end{lstlisting}
%
% // read one record
% CharString meta;
% DnaString seq;
% readRecord(meta, seq, reader);
%
% // read the entire file into optimized StringSets
%
% \end{lstlisting}
\section{Overview of new Stream Module}
There is a new Stream module, which currently resides in extras. The module
is composed of these main components:
\begin{itemize}
\item Stream related (basic abstraction for IO)
\begin{itemize}
\item the stream concept [\verb#concept_stream.h#]
\item Stream adaptations [\verb#adapt_fstream.h#, \verb#adapt_cstdio.h# ..]
\item Stream class and specializations [\verb#stream_*.h#]
\end{itemize}
\item reading related
\begin{itemize}
\item core RecordReader classes (generic Buffer around Stream) [\verb#record_reader*.h#]
\item tokenizing code (low level readUntil* , readWhile* calls etc)
[\verb#tokenizing.h# \verb#is.h#]
\item Format related reading based on tokenizing and RecordReader [\verb#read*.h#]
\item File format detection based on above code [\verb#guess_stream_format.h#]
\end{itemize}
\item Format related writing code [\verb#write*.h#]
\item code for casting from string to numerical types [\verb#lexical_cast.h#]
\end{itemize}
\section{Notes on completing the IO-Revision}
\subsection{General Information}
\begin{itemize}
\item different Stream backends are not yet implemented (e.g. char array, iostream)
\item \verb#tokenizing.h# contains copies of all old parsing/tokenizing functions, please
have a look there when switching to new IO (especially since in the old IO everyone
confused 'blank' and 'whitespace', as well as other terms!). Please also add any
tokenizing functions to \verb#tokenizing.h# and not into your file format code. Do
also check if you can use the helper-functions in \verb#tokenizing.h# for your task!
\item once all code is ported read2() and write2() shall be renamed to read() and
write()
\end{itemize}
Throughout SeqAn, IO-related code is marked with \verb#//IOREV# and zero or more
of the following tags:
\begin{tabular}{ll}\toprule
\textsc{Tag} & \textsc{Description} \\\midrule
\verb#_nottested_# &This code may not work at all \\
\verb#_docnottested_# &This code is documented but may behave different from doc\\
\verb#_nodoc_# &This code should be documented but isn't\\
\verb#_duplicate_# &This code reimplements functionality found elsewhere\\
\verb#_delcandidate_# &This code should probably just be deleted\\
\verb#_notIO_# &This code is falsely tagged as IO\\
\verb#_noop_# &This call doesnt do anything\\
\verb#_stub_# &This looks like its not done yet\\\bottomrule
\end{tabular}
I would recommend first following the advice in this document and \textbf{after}
having
implemented/ported the relevant code portions, to grep through seqan for IOREV
and deal with the leftover code on an individual basis. Since tagging happened
in the beginning of my work, some code comments might not by applicable anymore.
\subsection{Open Issues in existing files}
\begin{tabular}{ll}\toprule
\textsc{File} & \textsc{TODOs} \\\midrule
\verb#tokenizing.h# &specialized comparison code for other alphabets beside Dna and Dna5\\
\verb#read_fasta_fastq.h# &Questions what kind of format behaviour is legal \\
&(empty sequences? empty headers?) \\\bottomrule
\end{tabular}
\subsection{Sequence IO}
The following previously supported file formats are still missing: embl, genbank, QSeq, raw, CGViz (write-only)
Once they are implemented and all applications have changed to new IO, the following
files will be obsolete:
\begin{packed_enum}
\item \verb#seqan/file/cstream.h#
\item \verb#seqan/file/stream.h#
\item \verb#seqan/file/file_base.h#
\item \verb#seqan/file/file_cstyle.h#
\item \verb#seqan/file/file_filereaderiterator.h#
\item \verb#seqan/file/file_filereader.h#
\item \verb#seqan/file/file_format.h#
\item \verb#seqan/file/stream_algorithms.h#
\item \verb#seqan/file/file_format_raw.h#
\item \verb#seqan/file/file_format_fasta.h#
\item \verb#seqan/file/file_format_embl.h#
\item \verb#seqan/file/file_format_genbank.h#
\item \verb#seqan/file/file_format_cgviz.h#
\item \verb#seqan/file/file_format_mmap.h#
\item \verb#seqan/sequence/sequence_stream.h#
\item \verb#seqan/misc/misc_parsing.h#
\end{packed_enum}
The following files shall not be removed, it needs to be decided where to put them in the future:
\begin{packed_enum}
\item \verb#seqan/file/chunk_collector.h#
\item \verb#seqan/file/file_page.h#
\item \verb#seqan/file/file_page_raid0.h#
\item \verb#seqan/file/string_external.h#
\item \verb#seqan/file/string_mmap.h#
\item \verb#seqan/file/file_array.h#
\item \verb#seqan/system/file_directory.h#
\item \verb#seqan/system/file_async.h#
\item \verb#seqan/system/file_sync.h#
\end{packed_enum}
\subsection{Store-IO}
It was decided that store-io should be ported and moved to the new stream module,
this covers \verb#seqan/store/store_io*#. Other than the beginning of a new
sam-implementation (\verb#seqan/stream/read_sam.h#) none of this has yet
happened.
\subsection{Alignment and Alignment Graph formats}
Includes TCoffeLib, BlastLib, MummerLib, NewickFormat (all in\\
\verb#seqan/graph/graph_align_tcoffee_io.h#) and fasta-alignment format
(in\\ \verb#seqan/graph/graph_align_tcoffee_io.h# AND in
\verb#seqan/file/file_format_fasta_align.h#).
These should probably be ported as well, but it was not yet decided whether
they should be moved to the stream-module or continue to reside in the
graph-module.
\end{document}
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