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//![include]
#include <iostream>
#include <seqan/seq_io.h>
#include <seqan/journaled_set.h>
using namespace seqan2;
//![include]
//![findPatternInReference]
template <typename TString, typename TPattern>
void findPatternInReference(String<int> & hits,
TString const & reference,
TPattern const & pattern)
{
// [A] Check whether pattern fits into the sequence.
if (length(pattern) > length(reference))
return;
// [B] Iterate over all positions at which the pattern might occur.
for (unsigned pos = 0; pos < length(reference) - length(pattern) + 1; ++pos)
{
bool isHit = true;
// [C] Evaluate all positions of the pattern until you find a mismatch or you have found a hit.
for (unsigned posPattern = 0; posPattern < length(pattern); ++posPattern)
{
if (pattern[posPattern] != reference[posPattern + pos])
{
isHit = false;
break;
}
}
// [D] Report begin position at which pattern matches the sequence.
if (isHit)
appendValue(hits, pos);
}
}
//![findPatternInReference]
//![searchPattern]
template <typename TString, typename TPattern>
void searchPattern(StringSet<String<int> > & hitSet,
StringSet<TString, Owner<JournaledSet> > const & journalSet,
TPattern const & pattern)
{
typedef StringSet<TString, Owner<JournaledSet> > TJournalSet;
typedef typename Host<TJournalSet const>::Type THost;
// Check for valid initial state.
if (empty(host(journalSet)))
{
std::cout << "No reference set. Aborted search!" << std::endl;
return;
}
// Reset the hitSet to avoid phantom hits coming from a previous search.
clear(hitSet);
resize(hitSet, length(journalSet) + 1);
// Access the reference sequence.
THost & globalRef = host(journalSet);
// Search for pattern in the reference sequence.
findPatternInReference(hitSet[0], globalRef, pattern);
// Search for pattern in the journaled sequences.
for (unsigned i = 0; i < length(journalSet); ++i)
{
// findPatternInJournalString(hitSet[i+1], journalSet[i], pattern, hitSet[0]);
}
}
//![searchPattern]
//![loadAndJoin]
template <typename TString, typename TSpec>
inline int
loadAndJoin(StringSet<TString, Owner<JournaledSet> > & journalSet,
SeqFileIn & databaseFile,
JoinConfig<TSpec> const & joinConfig)
{
typedef typename Host<TString>::Type THost;
clear(journalSet);
String<char> seqId;
THost sequence;
// No sequences in the fasta file!
if (atEnd(databaseFile))
{
std::cerr << "Empty FASTA file." << std::endl;
return -1;
}
// First read sequence for reference sequence.
readRecord(seqId, sequence, databaseFile);
// We have to create the global reference sequence otherwise we loose the information after this function terminates.
createHost(journalSet, sequence);
// If there are more
while (!atEnd(databaseFile))
{
readRecord(seqId, sequence, databaseFile);
appendValue(journalSet, TString(sequence));
join(journalSet, length(journalSet) - 1, joinConfig);
}
return 0;
}
//![loadAndJoin]
//![main]
int main()
{
// Definition of the used types.
typedef String<Dna, Alloc<> > TSequence;
typedef String<Dna, Journaled<Alloc<>, SortedArray, Alloc<> > > TJournal;
typedef StringSet<TJournal, Owner<JournaledSet> > TJournaledSet;
// Open the stream to the file containing the sequences.
CharString seqDatabasePath = getAbsolutePath("demos/tutorial/journaled_set/sequences.fasta");
SeqFileIn databaseFile(toCString(seqDatabasePath));
// Reading each sequence and journal them.
TJournaledSet journalSet;
JoinConfig<GlobalAlign<JournaledCompact> > joinConfig;
loadAndJoin(journalSet, databaseFile, joinConfig);
// Define a pattern and start search.
StringSet<String<int> > hitSet;
TSequence pattern = "GTGGT";
std::cout << "Search for: " << pattern << ":\n";
searchPattern(hitSet, journalSet, pattern);
//![main]
//![printResult]
if (empty(hitSet[0]))
{
std::cout << "No hit in reference " << std::endl;
}
else
{
std::cout << "Hit in reference " << " at ";
for (unsigned j = 0; j < length(hitSet[0]); ++j)
std::cout << hitSet[0][j] << ": " << infix(host(journalSet), hitSet[0][j], hitSet[0][j] + length(pattern)) << "\t";
}
std::cout << std::endl;
std::cout << "Done!" << std::endl;
return 0;
}
//![printResult]
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