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// ==========================================================================
// SeqAn - The Library for Sequence Analysis
// ==========================================================================
// Copyright (c) 2006-2026, Knut Reinert, FU Berlin
// All rights reserved.
//
// Redistribution and use in source and binary forms, with or without
// modification, are permitted provided that the following conditions are met:
//
// * Redistributions of source code must retain the above copyright
// notice, this list of conditions and the following disclaimer.
// * Redistributions in binary form must reproduce the above copyright
// notice, this list of conditions and the following disclaimer in the
// documentation and/or other materials provided with the distribution.
// * Neither the name of Knut Reinert or the FU Berlin nor the names of
// its contributors may be used to endorse or promote products derived
// from this software without specific prior written permission.
//
// THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS"
// AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE
// IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE
// ARE DISCLAIMED. IN NO EVENT SHALL KNUT REINERT OR THE FU BERLIN BE LIABLE
// FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL
// DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR
// SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER
// CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT
// LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY
// OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH
// DAMAGE.
//
// ==========================================================================
// Author: David Weese <david.weese@fu-berlin.de>
// ==========================================================================
#ifndef INCLUDE_SEQAN_BAM_IO_BAM_SCANNER_CACHE_H_
#define INCLUDE_SEQAN_BAM_IO_BAM_SCANNER_CACHE_H_
#include <functional>
#include <unordered_map>
namespace seqan2 {
using namespace std;
// ============================================================================
// Forwards
// ============================================================================
// ============================================================================
// Tags, Classes, Enums
// ============================================================================
struct BamScannerCacheKey_
{
int32_t rID;
int32_t beginPos;
uint64_t qnameHash;
bool operator== (BamScannerCacheKey_ const &other) const
{
return rID == other.rID && beginPos == other.beginPos && qnameHash == other.qnameHash;
}
};
struct BamScannerCacheSearchKey_
{
typedef uint16_t TFlag;
BamScannerCacheKey_ cacheKey;
TFlag flags;
TFlag flagsMask;
};
struct BamScannerCacheHash_ :
std::function<size_t(BamScannerCacheKey_)>
{
size_t operator()(BamScannerCacheKey_ const &v) const
{
return std::hash<int32_t>()(v.rID) ^ std::hash<int32_t>()(v.beginPos) ^ std::hash<uint64_t>()(v.qnameHash);
}
};
class BamScannerCache
{
public:
// The Key is a pair of (genomic pos, name) where genomic pos is a pair of (rId, pos).
typedef String<BamAlignmentRecord> TRecords;
typedef Size<TRecords>::Type TRecordId;
typedef BamScannerCacheKey_ TKey;
// A mapping from the key type to the BamAlignmentRecord at this position.
typedef unordered_multimap<TKey, TRecordId, BamScannerCacheHash_> TMap;
typedef TMap::const_iterator TMapIter;
TRecords records;
String<TRecordId> unusedIds;
TMap map;
BamAlignmentRecord tmpRecord;
static const TRecordId INVALID_ID = (TRecordId)-1;
};
// ============================================================================
// Metafunctions
// ============================================================================
// ============================================================================
// Functions
// ============================================================================
template <typename TSequence>
uint64_t _suffixHash(TSequence const &sequence)
{
typedef typename Iterator<TSequence const, Standard>::Type TIter;
typedef typename Value<TSequence>::Type TValue;
typedef typename Size<TSequence>::Type TSize;
const uint64_t ALPH_SIZE = ValueSize<TValue>::VALUE;
const unsigned MAX_LEN = LogN<~(ALPH_SIZE - 1) / ALPH_SIZE, ALPH_SIZE>::VALUE + 1;
TSize len = length(sequence);
TIter itEnd = end(sequence, Standard());
if (len > 2 && (sequence[len - 2] == ':' || sequence[len - 2] == '/'))
{
len -= 2;
itEnd -= 2;
}
if (len > MAX_LEN)
len = MAX_LEN;
TIter it = itEnd - len;
uint64_t hash = 0;
for (; it != itEnd; ++it)
hash = hash * ALPH_SIZE + ordValue(*it);
return hash;
}
inline void
insertRecord(BamScannerCache &cache, seqan2::BamAlignmentRecord const &record)
{
int _id;
if (empty(cache.unusedIds))
{
_id = length(cache.records);
appendValue(cache.records, record);
}
else
{
_id = back(cache.unusedIds);
eraseBack(cache.unusedIds);
cache.records[_id] = record;
}
BamScannerCache::TKey key = { record.rID, record.beginPos, _suffixHash(record.qName) };
cache.map.insert(std::make_pair(key, _id));
}
template <typename TQName1, typename TQName2>
inline bool
_qNamesEqual(TQName1 const &name1, TQName2 const &name2)
{
unsigned len1 = length(name1);
unsigned len2 = length(name2);
if (len1 != len2)
return false;
if (len1 > 2 && len2 > 2)
{
if (name1[len1 - 2] == ':' || name1[len1 - 2] == '/')
return prefix(name1, len1 - 1) == prefix(name2, len2 - 1);
}
return name1 == name2;
}
// search a certain segment that might refer to lastRecord
// if recursively search for a intermediate segments that might refer to lastRecord
inline bool
_recursivelyFindSegmentGraph(
String<BamAlignmentRecord> &records,
BamScannerCacheSearchKey_ &searchKey,
unsigned segmentNo,
BamScannerCache &cache)
{
typedef BamScannerCache::TMapIter TMapIter;
typedef BamScannerCacheSearchKey_::TFlag TFlag;
// search for segment using contigId, position
std::pair<TMapIter, TMapIter> range = cache.map.equal_range(searchKey.cacheKey);
for (TMapIter iter = range.first; iter != range.second;)
{
TMapIter it = iter;
++iter;
BamAlignmentRecord &record = cache.records[it->second];
// ... additionally check flags and qName
if ((record.flag & searchKey.flagsMask) == searchKey.flags &&
_qNamesEqual(record.qName, records[0].qName))
{
if (record.rNextId == records[0].rID && record.pNext == records[0].beginPos)
{
resize(records, segmentNo + 2);
records[segmentNo + 1] = records[0];
records[segmentNo] = record;
appendValue(cache.unusedIds, it->second);
cache.map.erase(it);
return true;
}
}
}
for (; range.first != range.second; ++range.first)
{
BamAlignmentRecord &record = cache.records[range.first->second];
// ... additionally check flags and qName
if ((record.flag & searchKey.flagsMask) == searchKey.flags &&
_qNamesEqual(record.qName, records[0].qName))
{
BamScannerCacheSearchKey_ newSearchKey = {
{ record.rNextId, record.pNext, searchKey.cacheKey.qnameHash },
static_cast<TFlag>((record.flag & BAM_FLAG_MULTIPLE) | ((record.flag & BAM_FLAG_NEXT_RC) >> 1)),
BAM_FLAG_MULTIPLE | BAM_FLAG_RC
};
if (_recursivelyFindSegmentGraph(records, newSearchKey, segmentNo + 1, cache))
{
records[segmentNo] = record;
appendValue(cache.unusedIds, range.first->second);
cache.map.erase(range.first);
return true;
}
}
}
return false;
}
inline void
readMultiRecords(String<BamAlignmentRecord> &records, BamFileIn &bamFile, BamScannerCache &cache)
{
typedef BamScannerCacheSearchKey_::TFlag TFlag;
if (empty(records))
resize(records, 1);
while (!atEnd(bamFile))
{
// read next record
BamAlignmentRecord &record = records[0];
readRecord(record, bamFile);
// is this a single-end read or single alignment?
if (!hasFlagMultiple(record) ||
record.rID == BamAlignmentRecord::INVALID_REFID ||
record.beginPos == BamAlignmentRecord::INVALID_POS ||
record.rNextId == BamAlignmentRecord::INVALID_REFID ||
record.pNext == BamAlignmentRecord::INVALID_POS)
{
resize(records, 1);
return;
}
if (record.rID < record.rNextId || (record.rID == record.rNextId && record.beginPos < record.pNext))
{
// store record to retrieve it later
insertRecord(cache, record);
continue;
}
// search mates in case of multiple templates
BamScannerCacheSearchKey_ searchKey = {
{ record.rNextId, record.pNext, _suffixHash(record.qName) },
static_cast<TFlag>((record.flag & BAM_FLAG_MULTIPLE) | ((record.flag & BAM_FLAG_NEXT_RC) >> 1)),
BAM_FLAG_MULTIPLE | BAM_FLAG_RC
};
if (_recursivelyFindSegmentGraph(records, searchKey, 0, cache))
{
// order paired-end reads by first and second segment in the template
// if (length(records) == 2)
// {
// if (hasFlagLast(records[0]))
// std::swap(records[0], records[1]);
// }
return;
}
else
{
// we could get here if both mates align to same position and we are the first
// hence, insert our record to be retrieved by the second.
if (records[0].beginPos != records[0].pNext)
{
std::cerr << "WARNING: Mate could not be found for:\n";
write(std::cerr, records[0], context(bamFile), seqan2::Sam());
}
insertRecord(cache, records[0]);
}
}
clear(records);
}
} // namespace seqan2;
#endif // #ifndef INCLUDE_SEQAN_BAM_IO_BAM_SCANNER_CACHE_H_
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