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// ==========================================================================
// SeqAn - The Library for Sequence Analysis
// ==========================================================================
// Copyright (c) 2006-2026, Knut Reinert, FU Berlin
// All rights reserved.
//
// Redistribution and use in source and binary forms, with or without
// modification, are permitted provided that the following conditions are met:
//
// * Redistributions of source code must retain the above copyright
// notice, this list of conditions and the following disclaimer.
// * Redistributions in binary form must reproduce the above copyright
// notice, this list of conditions and the following disclaimer in the
// documentation and/or other materials provided with the distribution.
// * Neither the name of Knut Reinert or the FU Berlin nor the names of
// its contributors may be used to endorse or promote products derived
// from this software without specific prior written permission.
//
// THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS"
// AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE
// IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE
// ARE DISCLAIMED. IN NO EVENT SHALL KNUT REINERT OR THE FU BERLIN BE LIABLE
// FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL
// DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR
// SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER
// CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT
// LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY
// OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH
// DAMAGE.
//
// ==========================================================================
// Author: Sebastian Proft <sebastian.proft@fu-berlin.de>
// Author: Anton Komissarov <anton.komissarov@fu-berlin.de>
// Author: Temesgen Dadi <temesgen.dadi@fu-berlin.de>
// ==========================================================================
// Input on BAM and SAM files.
// ==========================================================================
#include <seqan/bam_io.h>
#ifndef SEQAN_SEQ_IO_BAM_SAM_H_
#define SEQAN_SEQ_IO_BAM_SAM_H_
namespace seqan2
{
// ----------------------------------------------------------------------------
// Class SamIgnoreFunctor_
// ----------------------------------------------------------------------------
template <typename TAlphabet>
struct SamIgnoreFunctor_
{
// ignore only newline if the target alphabet is a char
// ignore whitespace as well for all other alphabets
typedef typename If<Or<IsSameType<TAlphabet, char>,
Or<IsSameType<TAlphabet, signed char>,
IsSameType<TAlphabet, unsigned char> > >,
IsNewline, IsWhitespace >::Type Type;
};
template <typename TAlphabet>
struct SamIgnoreOrAssertFunctor_
{
typedef typename SamIgnoreFunctor_<TAlphabet>::Type TIgnore;
typedef AssertFunctor<IsInAlphabet<TAlphabet>, ParseError, Sam> TAsserter;
// don't assert in case of char alphabets
// assert being part of the alphabet for other alphabets
typedef typename If<Or<IsSameType<TAlphabet, char>,
Or<IsSameType<TAlphabet, signed char>,
IsSameType<TAlphabet, unsigned char> > >,
TIgnore, OrFunctor<TIgnore, TAsserter> >::Type Type;
};
// ============================================================================
// Functions
// ============================================================================
// ----------------------------------------------------------------------------
// Function _skipHeader() SamHeader
// ----------------------------------------------------------------------------
template <typename TFile>
inline void _skipHeader(TFile & file, Sam const & /* format */)
{
while (nextIs(file.iter, SamHeader()))
{
skipLine(file.iter);
}
}
// ----------------------------------------------------------------------------
// Function _skipHeader() BamHeader
// ----------------------------------------------------------------------------
template <typename TFile>
inline void _skipHeader(TFile & file, Bam const & /* format */)
{
BamHeader header;
BamFileIn bamIn;
open(bamIn, file.stream);
file.context.bamIOContext = context(bamIn);
readHeader(header, file.context.bamIOContext, file.iter, Bam());
}
// ----------------------------------------------------------------------------
// Function readRecord Bam
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TCharIter>
inline int32_t readBamRecord(TIdString & meta,
TSeqString & seq,
TIdString & prevQName,
int32_t & remainingBytes,
TCharIter & it)
{
typedef typename Iterator<TSeqString, Standard>::Type TSeqIter;
// BamAlignmentRecordCore.
BamAlignmentRecordCore recordCore;
arrayCopyForward(it, it + sizeof(BamAlignmentRecordCore), reinterpret_cast<char *>(&recordCore));
enforceLittleEndian(recordCore);
it += sizeof(BamAlignmentRecordCore);
clear(meta);
clear(seq);
remainingBytes -= sizeof(BamAlignmentRecordCore) + recordCore._l_qname +
recordCore._n_cigar * 4 + (recordCore._l_qseq + 1) / 2 + recordCore._l_qseq;
SEQAN_ASSERT_GEQ(remainingBytes, 0);
// query name.
resize(meta, recordCore._l_qname - 1, Exact());
arrayCopyForward(it, it + recordCore._l_qname - 1, begin(meta, Standard()));
it += recordCore._l_qname;
if (prevQName == meta)
return 0;
// skip cigar string.
it += sizeof(uint32_t) * recordCore._n_cigar;
// query sequence.
resize(seq, recordCore._l_qseq, Exact());
TSeqIter sit = begin(seq, Standard());
TSeqIter sitEnd = sit + (recordCore._l_qseq & ~1);
while (sit != sitEnd)
{
unsigned char ui = getValue(it);
++it;
*sit = Iupac(ui >> 4);
++sit;
*sit = Iupac(ui & 0x0f);
++sit;
}
if (recordCore._l_qseq & 1)
*sit++ = Iupac((uint8_t)*it++ >> 4);
return recordCore._l_qseq; // returns length of query sequence. Needed for reading quality
}
// ----------------------------------------------------------------------------
// Function readRecord
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TForwardIter>
inline bool readSamRecord(TIdString & meta,
TSeqString & seq,
TIdString & prevQName,
TForwardIter & iter)
{
typedef typename Value<TSeqString>::Type TSeqAlphabet;
typedef typename SamIgnoreOrAssertFunctor_<TSeqAlphabet>::Type TSeqIgnoreOrAssert;
// fail, if we read "@" (did you miss to call readRecord(header, bamFile) first?)
if (nextIs(iter, SamHeader()))
SEQAN_THROW(ParseError("Unexpected SAM header encountered."));
OrFunctor<IsTab, AssertFunctor<NotFunctor<IsNewline>, ParseError, Sam> > nextEntry;
clear(meta);
clear(seq);
// QNAME
readUntil(meta, iter, nextEntry);
skipOne(iter, IsTab());
// if we have seen this sequence before, return
if (meta == prevQName)
return false;
// Skip FLAG, RNAME, POS, MAPQ, CIGAR, RNEXT, PNEXT, TLEN
for (unsigned i = 0; i < 8; ++i)
{
skipUntil(iter, IsTab());
skipOne(iter, IsTab());
}
// SEQ
readUntil(seq, iter, nextEntry, TSeqIgnoreOrAssert());
// Handle case of missing sequence: Clear seq string as documented.
if (seq == "*")
clear(seq);
skipOne(iter, IsTab());
return true;
}
template <typename TIdString, typename TSeqString, typename TQualString, typename TFile>
inline void _readRecord(TIdString & meta,
TSeqString & seq,
TQualString & qual,
TFile & file,
Sam const & /* format */)
{
typedef typename Value<TQualString>::Type TQualAlphabet;
typedef typename SamIgnoreOrAssertFunctor_<TQualAlphabet>::Type TQualIgnoreOrAssert;
if (SEQAN_UNLIKELY(!(context(file).headerWasRead)))
{
_skipHeader(file, Sam());
file.context.headerWasRead = true;
}
TIdString pId = toCString(file.context.prevId);
clear(qual);
if (readSamRecord(meta, seq, pId, file.iter))
{
// QUAL
readUntil(qual, file.iter, OrFunctor<IsTab, IsNewline>(), TQualIgnoreOrAssert());
clear(file.context.prevId);
file.context.prevId = meta;
}
else
{
clear(file.context.prevId);
file.context.prevId = meta;
clear(meta);
}
skipLine(file.iter);
}
template <typename TIdString, typename TSeqString, typename TQualString, typename TFile>
inline void _readRecord(TIdString & meta,
TSeqString & seq,
TQualString & qual,
TFile & file,
Bam const & /* format */)
{
typedef typename Iterator<CharString, Standard>::Type TCharIter;
typedef typename Iterator<TQualString, Standard>::Type SEQAN_RESTRICT TQualIter;
if (SEQAN_UNLIKELY(!(context(file).headerWasRead)))
{
_skipHeader(file, Bam());
file.context.headerWasRead = true;
}
TIdString pId = toCString(file.context.prevId);
clear(qual);
// Read size and data of the remaining block in one chunk (fastest).
int32_t remainingBytes = _readBamRecordWithoutSize(file.context.bamIOContext.buffer, file.iter);
TCharIter it = begin(file.context.bamIOContext.buffer, Standard());
if (int32_t l_qseq = readBamRecord(meta, seq, pId, remainingBytes, it))
{
// phred quality
resize(qual, l_qseq, Exact());
TQualIter qitEnd = end(qual, Standard());
for (TQualIter qit = begin(qual, Standard()); qit != qitEnd; )
{
*qit++ = '!' + *it++;
}
clear(file.context.prevId);
file.context.prevId = meta;
}
else
{
clear(file.context.prevId);
file.context.prevId = meta;
clear(meta);
}
// skip tags
it += remainingBytes;
}
// ----------------------------------------------------------------------------
// Function readRecord(SAM); With qualities
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TQualString, typename TSpec>
inline SEQAN_FUNC_ENABLE_IF(Is<InputStreamConcept<typename FormattedFile<Fastq, Input, TSpec>::TStream> >, void)
readRecord(TIdString & meta,
TSeqString & seq,
TQualString & qual,
FormattedFile<Fastq, Input, TSpec> & file,
Sam const & /* format */)
{
clear(qual);
_readRecord(meta, seq, qual, file, Sam());
}
// ----------------------------------------------------------------------------
// Function readRecord(SAM); Without qualities
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TSpec>
inline SEQAN_FUNC_ENABLE_IF(Is<InputStreamConcept<typename FormattedFile<Fastq, Input, TSpec>::TStream> >, void)
readRecord(TIdString & meta,
TSeqString & seq,
FormattedFile<Fastq, Input, TSpec> & file,
Sam const & /* format */)
{
readRecord(meta, seq, context(file).buffer[2], file, Sam());
}
// ----------------------------------------------------------------------------
// Function readRecord(BAM); With qualities
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TQualString, typename TSpec>
inline SEQAN_FUNC_ENABLE_IF(Is<InputStreamConcept<typename FormattedFile<Fastq, Input, TSpec>::TStream> >, void)
readRecord(TIdString & meta,
TSeqString & seq,
TQualString & qual,
FormattedFile<Fastq, Input, TSpec> & file,
Bam const & /* format */)
{
clear(qual);
_readRecord(meta, seq, qual, file, Bam());
}
// ----------------------------------------------------------------------------
// Function readRecord(BAM); Without qualities
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TSpec>
inline SEQAN_FUNC_ENABLE_IF(Is<InputStreamConcept<typename FormattedFile<Fastq, Input, TSpec>::TStream> >, void)
readRecord(TIdString & meta,
TSeqString & seq,
FormattedFile<Fastq, Input, TSpec> & file,
Bam const & /* format */)
{
readRecord(meta, seq, context(file).buffer[2], file, Bam());
}
inline void fillHeader(BamHeader & header)
{
typedef BamHeaderRecord::TTag TTag;
BamHeaderRecord seqRecord;
// Fill first header line.
BamHeaderRecord firstRecord;
firstRecord.type = BAM_HEADER_FIRST;
appendValue(firstRecord.tags, TTag("VN", "2.3"));
appendValue(firstRecord.tags, TTag("SO", "sorted"));
appendValue(header, firstRecord);
// Fill program header line.
BamHeaderRecord pgRecord;
pgRecord.type = BAM_HEADER_PROGRAM;
appendValue(pgRecord.tags, TTag("ID", "SeqAn_IO"));
appendValue(pgRecord.tags, TTag("PN", "SeqAn_IO"));
appendValue(header, pgRecord);
// Fill read group header line.
BamHeaderRecord rgRecord;
rgRecord.type = BAM_HEADER_READ_GROUP;
appendValue(rgRecord.tags, TTag("ID", "none"));
appendValue(rgRecord.tags, TTag("SM", "none"));
appendValue(rgRecord.tags, TTag("PG", "SeqAn_IO"));
appendValue(header, rgRecord);
}
// ----------------------------------------------------------------------------
// Function _writeRecord(SAM);
// ----------------------------------------------------------------------------
template <typename TFile, typename TIdString, typename TSeqString, typename TQualString>
inline void _writeRecord(TFile & file,
TIdString const & meta,
TSeqString const & seq,
TQualString const & qual,
Sam const & /* format */)
{
if (SEQAN_UNLIKELY(!(context(file).headerWasWriten)))
{
BamHeader header;
fillHeader(header);
StringSet<CharString> contigNameStore;
appendValue(contigNameStore, "*");
NameStoreCache<StringSet<CharString> > contigNameStoreCache(contigNameStore);
BamIOContext<StringSet<CharString> > bamIOContext(contigNameStore, contigNameStoreCache);
file.context.bamIOContext = bamIOContext;
write(file.iter, header, context(file).bamIOContext, Sam());
file.context.headerWasWriten = true;
}
BamAlignmentRecord rec;
clear(rec);
rec.qName = meta;
rec.seq = seq;
rec.qual = qual;
rec.flag = BAM_FLAG_UNMAPPED;
write(file.iter, rec, context(file).bamIOContext, Sam());
}
// ----------------------------------------------------------------------------
// Function _writeRecord(BAM);
// ----------------------------------------------------------------------------
template <typename TFile, typename TIdString, typename TSeqString, typename TQualString>
inline void _writeRecord(TFile & file,
TIdString const & meta,
TSeqString const & seq,
TQualString const & qual,
Bam const & /* format */)
{
if (SEQAN_UNLIKELY(!(context(file).headerWasWriten)))
{
BamHeader header;
fillHeader(header);
StringSet<CharString> contigNameStore;
appendValue(contigNameStore, "*");
NameStoreCache<StringSet<CharString> > contigNameStoreCache(contigNameStore);
BamIOContext<StringSet<CharString> > bamIOContext(contigNameStore, contigNameStoreCache);
file.context.bamIOContext = bamIOContext;
write(file.iter, header, context(file).bamIOContext, Bam());
file.context.headerWasWriten = true;
}
BamAlignmentRecord rec;
clear(rec);
rec.qName = meta;
rec.seq = seq;
rec.qual = qual;
rec.flag = BAM_FLAG_UNMAPPED;
write(file.iter, rec, context(file).bamIOContext, Bam());
}
// ----------------------------------------------------------------------------
// Function writeRecord(SAM); Separate Qualities
// ----------------------------------------------------------------------------
template <typename TSpec, typename TIdString, typename TSeqString, typename TQualString>
inline void writeRecord(FormattedFile<Fastq, Output, TSpec> & file,
TIdString const & meta,
TSeqString const & seq,
TQualString const & qual,
Sam const & /* format */)
{
_writeRecord(file, meta, seq, qual, Sam());
}
// ----------------------------------------------------------------------------
// Function writeRecord(SAM); Qualities inside seq
// ----------------------------------------------------------------------------
template <typename TSpec, typename TIdString, typename TSeqString>
inline SEQAN_FUNC_ENABLE_IF(HasQualities<typename Value<TSeqString>::Type> , void)
writeRecord(FormattedFile<Fastq, Output, TSpec> & file,
TIdString const & meta,
TSeqString const & seq,
Sam const & /* format */)
{
typedef QualityExtractor<typename Value<TSeqString>::Type> TQualityExtractor;
ModifiedString<TSeqString const, ModView<TQualityExtractor> > quals(seq);
writeRecord(file, meta, seq, quals, Sam());
}
// ----------------------------------------------------------------------------
// Function writeRecord(SAM); Without qualities inside seq
// ----------------------------------------------------------------------------
template <typename TSpec, typename TIdString, typename TSeqString>
inline SEQAN_FUNC_ENABLE_IF(Not<HasQualities<typename Value<TSeqString>::Type> >, void)
writeRecord(FormattedFile<Fastq, Output, TSpec> & file,
TIdString const & meta,
TSeqString const & seq,
Sam const & /* format */)
{
writeRecord(file, meta, seq, "*", Sam());
}
// ----------------------------------------------------------------------------
// Function writeRecord(BAM); Separate Qualities
// ----------------------------------------------------------------------------
template <typename TSpec, typename TIdString, typename TSeqString, typename TQualString>
inline void writeRecord(FormattedFile<Fastq, Output, TSpec> & file,
TIdString const & meta,
TSeqString const & seq,
TQualString const & qual,
Bam const & /* format */)
{
_writeRecord(file, meta, seq, qual, Bam());
}
// ----------------------------------------------------------------------------
// Function writeRecord(BAM); Qualities inside seq
// ----------------------------------------------------------------------------
template <typename TSpec, typename TIdString, typename TSeqString>
inline SEQAN_FUNC_ENABLE_IF(HasQualities<typename Value<TSeqString>::Type> , void)
writeRecord(FormattedFile<Fastq, Output, TSpec> & file,
TIdString const & meta,
TSeqString const & seq,
Bam const & /* format */)
{
typedef QualityExtractor<typename Value<TSeqString>::Type> TQualityExtractor;
ModifiedString<TSeqString const, ModView<TQualityExtractor> > quals(seq);
CharString qual = quals;
writeRecord(file, meta, seq, quals, Bam());
}
// ----------------------------------------------------------------------------
// Function writeRecord(BAM); Without qualities inside seq
// ----------------------------------------------------------------------------
template <typename TSpec, typename TIdString, typename TSeqString>
inline SEQAN_FUNC_ENABLE_IF(Not<HasQualities<typename Value<TSeqString>::Type> >, void)
writeRecord(FormattedFile<Fastq, Output, TSpec> & file,
TIdString const & meta,
TSeqString const & seq,
Bam const & /* format */)
{
writeRecord(file, meta, seq, "*", Bam());
}
// ----------------------------------------------------------------------------
// Function readRecord(Bam) -> Directly from an iterator -> not Implemented
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TIterator>
inline void readRecord(TIdString & /* meta */, TSeqString & /* seq */, TIterator & /* iter */, Bam const & /* format */)
{}
// ----------------------------------------------------------------------------
// Function readRecord(Bam) -> Directly from an iterator -> not Implemented
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TQualString, typename TIterator>
inline void readRecord(TIdString & /* meta */,
TSeqString & /* seq */,
TQualString & /* qual */,
TIterator & /* iter */,
Bam const & /* format */)
{}
// ----------------------------------------------------------------------------
// Function readRecord(Sam) -> Directly from an iterator -> not Implemented
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TIterator>
inline void readRecord(TIdString & /* meta */, TSeqString & /* seq */, TIterator & /* iter */, Sam const & /* format */)
{}
// ----------------------------------------------------------------------------
// Function readRecord(Sam) -> Directly from an iterator -> not Implemented
// ----------------------------------------------------------------------------
template <typename TIdString, typename TSeqString, typename TQualString, typename TIterator>
inline void
readRecord(TIdString & /* meta */,
TSeqString & /* seq */,
TQualString & /* qual */,
TIterator & /* iter */,
Sam const & /* format */)
{}
} // namespace seqan2
#endif // #ifndef SEQAN_SEQ_IO_BAM_SAM_H_
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