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|
// -----------------------------------------------------------------------------------------------------
// Copyright (c) 2006-2020, Knut Reinert & Freie Universität Berlin
// Copyright (c) 2016-2020, Knut Reinert & MPI für molekulare Genetik
// This file may be used, modified and/or redistributed under the terms of the 3-clause BSD-License
// shipped with this file and also available at: https://github.com/seqan/seqan3/blob/master/LICENSE.md
// -----------------------------------------------------------------------------------------------------
#include <sstream>
#include <gtest/gtest.h>
#include <seqan3/alphabet/quality/phred42.hpp>
#include <seqan3/io/alignment_file/input.hpp>
#include <seqan3/range/views/convert.hpp>
#include <seqan3/std/algorithm>
#include <seqan3/std/iterator>
#include <seqan3/std/ranges>
#include <seqan3/test/expect_range_eq.hpp>
#include <seqan3/test/tmp_filename.hpp>
using seqan3::operator""_dna4;
using seqan3::operator""_dna5;
using seqan3::operator""_phred42;
using default_fields = seqan3::fields<seqan3::field::seq, seqan3::field::id, seqan3::field::qual>;
TEST(alignment_file_input_iterator, concepts)
{
using it_t = typename seqan3::alignment_file_input<>::iterator;
using sen_t = typename seqan3::alignment_file_input<>::sentinel;
EXPECT_TRUE((std::input_iterator<it_t>));
EXPECT_TRUE((std::sentinel_for<sen_t, it_t>));
}
struct alignment_file_input_f : public ::testing::Test
{
std::string input =
R"(@HD VN:1.6 SO:unknown GO:none
@SQ SN:ref LN:34
@PG ID:prog1 PN:cool_program
@CO This is a comment.
read1 41 ref 1 61 1S1M1D2M ref 10 300 ACGT !##$ AS:i:2 NM:i:7
read2 42 ref 2 62 7M1D1M1S ref 10 300 AGGCTGNAG !##$&'()* xy:B:S,3,4,5
read3 43 ref 3 63 1S1M1D4M1D1M1S ref 10 300 GGAGTATA !!*+,-./
)";
std::vector<seqan3::dna5_vector> seq_comp
{
"ACGT"_dna5,
"AGGCTGNAG"_dna5,
"GGAGTATA"_dna5
};
std::vector<std::string> id_comp
{
"read1",
"read2",
"read3"
};
std::vector<std::vector<seqan3::phred42>> qual_comp
{
{ "!##$"_phred42 },
{ "!##$&'()*"_phred42 },
{ "!!*+,-./"_phred42 },
};
};
TEST_F(alignment_file_input_f, concepts)
{
using t = seqan3::alignment_file_input<>;
EXPECT_TRUE((std::ranges::input_range<t>));
using ct = seqan3::alignment_file_input<> const;
// not const-iterable
EXPECT_FALSE((std::ranges::input_range<ct>));
}
TEST_F(alignment_file_input_f, construct_by_filename)
{
/* just the filename */
{
seqan3::test::tmp_filename filename{"alignment_file_input_constructor.sam"};
{
std::ofstream filecreator{filename.get_path(), std::ios::out | std::ios::binary};
}
EXPECT_NO_THROW(seqan3::alignment_file_input<>{filename.get_path()} );
}
// correct format check is done by tests of that format
/* wrong extension */
{
seqan3::test::tmp_filename filename{"alignment_file_input_constructor.xyz"};
std::ofstream filecreator{filename.get_path(), std::ios::out | std::ios::binary};
EXPECT_THROW(seqan3::alignment_file_input<>{filename.get_path()},
seqan3::unhandled_extension_error );
}
/* non-existent file*/
{
EXPECT_THROW(seqan3::alignment_file_input<>{"/dev/nonexistent/foobarOOO"}, seqan3::file_open_error);
}
/* non-existent file with reference information*/
{
std::vector<std::string> ref_ids{"ref1", "ref2"};
std::vector<seqan3::dna4_vector> ref_seqs{"ACTG"_dna4, "ACTG"_dna4};
EXPECT_THROW((seqan3::alignment_file_input{"/dev/nonexistent/foobarOOO", ref_ids, ref_seqs}),
seqan3::file_open_error);
}
/* filename + fields */
{
seqan3::test::tmp_filename filename{"alignment_file_input_constructor.sam"};
{
std::ofstream filecreator{filename.get_path(), std::ios::out | std::ios::binary};
}
using fields_seq = seqan3::fields<seqan3::field::seq>;
EXPECT_NO_THROW(( seqan3::alignment_file_input<seqan3::alignment_file_input_default_traits<>,
fields_seq,
seqan3::type_list<seqan3::format_sam>>{filename.get_path(),
fields_seq{}} ));
}
}
TEST_F(alignment_file_input_f, construct_from_stream)
{
/* stream + format_tag */
EXPECT_NO_THROW(( seqan3::alignment_file_input<seqan3::alignment_file_input_default_traits<>,
default_fields,
seqan3::type_list<seqan3::format_sam>>{std::istringstream{input},
seqan3::format_sam{}} ));
/* stream + format_tag + fields */
EXPECT_NO_THROW(( seqan3::alignment_file_input<seqan3::alignment_file_input_default_traits<>,
default_fields,
seqan3::type_list<seqan3::format_sam>>{std::istringstream{input},
seqan3::format_sam{},
default_fields{}} ));
}
TEST_F(alignment_file_input_f, default_template_args_and_deduction_guides)
{
using comp0 = seqan3::alignment_file_input_default_traits<>;
using comp1 = seqan3::fields<seqan3::field::seq,
seqan3::field::id,
seqan3::field::offset,
seqan3::field::ref_seq,
seqan3::field::ref_id,
seqan3::field::ref_offset,
seqan3::field::alignment,
seqan3::field::cigar,
seqan3::field::mapq,
seqan3::field::qual,
seqan3::field::flag,
seqan3::field::mate,
seqan3::field::tags,
seqan3::field::evalue,
seqan3::field::bit_score,
seqan3::field::header_ptr>;
using comp2 = seqan3::type_list<seqan3::format_sam, seqan3::format_bam>;
using comp3 = char;
/* default template args */
{
using t = seqan3::alignment_file_input<>;
EXPECT_TRUE((std::is_same_v<typename t::traits_type, comp0>));
EXPECT_TRUE((std::is_same_v<typename t::selected_field_ids, comp1>));
EXPECT_TRUE((std::is_same_v<typename t::valid_formats, comp2>));
EXPECT_TRUE((std::is_same_v<typename t::stream_char_type, comp3>));
}
/* guided filename constructor */
{
seqan3::test::tmp_filename filename{"alignment_file_input_constructor.sam"};
{
std::ofstream filecreator{filename.get_path(), std::ios::out | std::ios::binary};
}
seqan3::alignment_file_input fin{filename.get_path()};
using t = decltype(fin);
EXPECT_TRUE((std::is_same_v<typename t::traits_type, comp0>));
EXPECT_TRUE((std::is_same_v<typename t::selected_field_ids, comp1>));
EXPECT_TRUE((std::is_same_v<typename t::valid_formats, comp2>));
EXPECT_TRUE((std::is_same_v<typename t::stream_char_type, comp3>));
}
/* guided filename constructor + custom fields */
{
seqan3::test::tmp_filename filename{"alignment_file_input_constructor.sam"};
{
std::ofstream filecreator{filename.get_path(), std::ios::out | std::ios::binary};
}
seqan3::alignment_file_input fin{filename.get_path(), seqan3::fields<seqan3::field::seq>{}};
using t = decltype(fin);
EXPECT_TRUE((std::is_same_v<typename t::traits_type, comp0>));
EXPECT_TRUE((std::is_same_v<typename t::selected_field_ids, seqan3::fields<seqan3::field::seq>>));
EXPECT_TRUE((std::is_same_v<typename t::valid_formats, comp2>));
EXPECT_TRUE((std::is_same_v<typename t::stream_char_type, comp3>));
}
/* guided stream constructor */
{
std::istringstream ext{input};
seqan3::alignment_file_input fin{ext, seqan3::format_sam{}};
using t = decltype(fin);
EXPECT_TRUE((std::is_same_v<typename t::traits_type, comp0>));
EXPECT_TRUE((std::is_same_v<typename t::selected_field_ids, comp1>));
EXPECT_TRUE((std::is_same_v<typename t::valid_formats, seqan3::type_list<seqan3::format_sam>>));
EXPECT_TRUE((std::is_same_v<typename t::stream_char_type, comp3>));
}
/* guided stream temporary constructor */
{
seqan3::alignment_file_input fin{std::istringstream{input}, seqan3::format_sam{}};
using t = decltype(fin);
EXPECT_TRUE((std::is_same_v<typename t::traits_type, comp0>));
EXPECT_TRUE((std::is_same_v<typename t::selected_field_ids, comp1>));
EXPECT_TRUE((std::is_same_v<typename t::valid_formats, seqan3::type_list<seqan3::format_sam>>));
EXPECT_TRUE((std::is_same_v<typename t::stream_char_type, comp3>));
}
}
TEST_F(alignment_file_input_f, empty_file)
{
seqan3::test::tmp_filename filename{"empty.sam"};
std::ofstream filecreator{filename.get_path(), std::ios::out | std::ios::binary};
seqan3::alignment_file_input fin{filename.get_path()};
EXPECT_EQ(fin.begin(), fin.end());
}
TEST_F(alignment_file_input_f, empty_stream)
{
seqan3::alignment_file_input fin{std::istringstream{std::string{}}, seqan3::format_sam{}};
EXPECT_EQ(fin.begin(), fin.end());
}
TEST_F(alignment_file_input_f, record_reading)
{
/* record based reading */
seqan3::alignment_file_input fin{std::istringstream{input}, seqan3::format_sam{}};
size_t counter = 0;
for (auto & rec : fin)
{
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::seq>(rec), seq_comp[counter]);
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::id>(rec), id_comp[counter]);
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::qual>(rec), qual_comp[counter]);
counter++;
}
EXPECT_EQ(counter, 3u);
}
TEST_F(alignment_file_input_f, record_reading_custom_fields)
{
/* record based reading */
seqan3::alignment_file_input fin{std::istringstream{input},
seqan3::format_sam{},
seqan3::fields<seqan3::field::id, seqan3::field::seq>{}};
size_t counter = 0;
for (auto & [ id, seq ] : fin)
{
EXPECT_RANGE_EQ(seq, seq_comp[counter]);
EXPECT_RANGE_EQ(id, id_comp[counter]);
counter++;
}
EXPECT_EQ(counter, 3u);
}
TEST_F(alignment_file_input_f, file_view)
{
seqan3::alignment_file_input fin{std::istringstream{input}, seqan3::format_sam{}};
#if !SEQAN3_WORKAROUND_GCC_93983
auto minimum_length_filter = std::views::filter([] (auto const & rec)
{
return size(seqan3::get<seqan3::field::seq>(rec)) >= 5;
});
#endif
size_t counter = 1; // the first record will be filtered out
#if SEQAN3_WORKAROUND_GCC_93983
for (auto & rec : fin /*| minimum_length_filter*/)
{
if (!(size(seqan3::get<seqan3::field::seq>(rec)) >= 5))
continue;
#else // ^^^ workaround / no workaround vvv
for (auto & rec : fin | minimum_length_filter)
{
#endif // SEQAN3_WORKAROUND_GCC_93983
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::seq>(rec), seq_comp[counter]);
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::id>(rec), id_comp[counter]);
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::qual>(rec), qual_comp[counter]);
counter++;
}
EXPECT_EQ(counter, 3u);
}
// ----------------------------------------------------------------------------
// decompression
// ----------------------------------------------------------------------------
template <typename fixture_t, typename input_file_t>
void decompression_impl(fixture_t & fix, input_file_t & fin)
{
size_t counter = 0;
for (auto & rec : fin)
{
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::seq>(rec), fix.seq_comp[counter]);
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::id>(rec), fix.id_comp[counter]);
EXPECT_RANGE_EQ(seqan3::get<seqan3::field::qual>(rec), fix.qual_comp[counter]);
counter++;
}
EXPECT_EQ(counter, 3u);
}
#ifdef SEQAN3_HAS_ZLIB
std::string input_gz
{
'\x1F','\x8B','\x08','\x08','\x9D','\x5B','\x38','\x5C','\x00','\x03','\x74','\x65','\x73','\x74','\x2E','\x73',
'\x61','\x6D','\x00','\x6D','\xCE','\xBF','\x0A','\xC2','\x30','\x10','\xC7','\xF1','\xF9','\xD7','\xB7','\x08',
'\x15','\xFF','\xD4','\xA8','\xB9','\x24','\xB6','\x90','\x2D','\x56','\xB8','\x29','\x5D','\x92','\x17','\x28',
'\x58','\xC1','\x35','\x93','\xBE','\xBD','\x21','\xBA','\x08','\x4E','\x07','\xC7','\x7D','\xBE','\x5C','\x5E',
'\xE6','\x1B','\xC1','\x12','\xF2','\x72','\x07','\xA1','\x27','\x50','\xA4','\x40','\x57','\x1D','\x3E','\x1B',
'\x05','\xA3','\x14','\xFC','\xC8','\x09','\xA2','\x6D','\x57','\xF0','\xD1','\x3D','\x9C','\xC6','\x14','\xCA',
'\x18','\x9A','\x5C','\xB4','\x86','\xD5','\xF5','\x56','\xA3','\xD7','\x18','\x8A','\x2D','\x3E','\xFE','\x68',
'\xE6','\x31','\xF1','\xE4','\xB9','\x26','\xD6','\x9B','\xED','\xAE','\xC3','\xF3','\xE5','\x2E','\x2E','\x4A',
'\x23','\xAD','\x3C','\xD7','\x8C','\x81','\x35','\x15','\x19','\xF4','\xE6','\xFB','\x84','\xFD','\x13','\x63',
'\xF6','\x9C','\x7C','\xF2','\x10','\xA2','\xDB','\xCB','\xC3','\xF1','\xD4','\xBC','\x01','\xDB','\x85','\xA3',
'\xD3','\xC3','\x00','\x00','\x00'
};
TEST_F(alignment_file_input_f, decompression_by_filename_gz)
{
seqan3::test::tmp_filename filename{"alignment_file_output_test.sam.gz"};
{
std::ofstream of{filename.get_path(), std::ios::binary};
std::copy(input_gz.begin(), input_gz.end(), std::ostreambuf_iterator<char>{of});
}
seqan3::alignment_file_input fin{filename.get_path()};
decompression_impl(*this, fin);
}
TEST_F(alignment_file_input_f, decompression_by_stream_gz)
{
seqan3::alignment_file_input fin{std::istringstream{input_gz}, seqan3::format_sam{}};
decompression_impl(*this, fin);
}
TEST_F(alignment_file_input_f, read_empty_gz_file)
{
std::string empty_zipped_file
{
'\x1f', '\x8b', '\x08', '\x08', '\x5a', '\x07', '\x98', '\x5c',
'\x00', '\x03', '\x66', '\x6f', '\x6f', '\x00', '\x03', '\x00',
'\x00', '\x00', '\x00', '\x00', '\x00', '\x00', '\x00', '\x00'
};
seqan3::alignment_file_input fin{std::istringstream{empty_zipped_file}, seqan3::format_sam{}};
EXPECT_TRUE(fin.begin() == fin.end());
}
std::string input_bgzf
{
'\x1F', '\x8B', '\x08', '\x04', '\x00', '\x00', '\x00', '\x00', '\x00', '\xFF', '\x06', '\x00', '\x42', '\x43',
'\x02', '\x00', '\xF2', '\x00', '\x6D', '\x8E', '\x5D', '\x4B', '\xC3', '\x40', '\x10', '\x45', '\x9F', '\x6F',
'\x7E', '\x45', '\x43', '\xC5', '\x6A', '\x8D', '\x31', '\xFB', '\x61', '\x0A', '\xF3', '\x94', '\x35', '\x81',
'\x55', '\x30', '\x9B', '\xCA', '\x06', '\x5F', '\x25', '\xD4', '\xA8', '\x45', '\xB3', '\x2B', '\x51', '\x51',
'\xFF', '\xBD', '\xE9', '\xEA', '\x8B', '\x50', '\x18', '\xB8', '\xDC', '\x61', '\xCE', '\x61', '\x8A', '\xCB',
'\x0A', '\xB7', '\x86', '\x58', '\x9A', '\xC3', '\x36', '\xF4', '\xE1', '\x9E', '\x9D', '\xFF', '\x74', '\xD0',
'\x0D', '\x39', '\xEF', '\xFA', '\xA8', '\xB0', '\x37', '\xB0', '\x86', '\xC6', '\xFE', '\x01', '\xD7', '\x86',
'\x84', '\x8C', '\x8A', '\xB5', '\xC6', '\x55', '\x45', '\xAF', '\xA3', '\x7F', '\x64', '\x58', '\x1B', '\xDA',
'\x78', '\xFF', '\x72', '\xB7', '\x6B', '\x63', '\x37', '\x44', '\x45', '\xD9', '\xA0', '\x7D', '\xDA', '\xBE',
'\xCD', '\xA6', '\xE9', '\x66', '\x1B', '\x3F', '\x0C', '\xBD', '\x7B', '\x4F', '\xA3', '\xB1', '\xEF', '\xEE',
'\x19', '\x24', '\xC3', '\x4E', '\xC3', '\x90', '\x33', '\x30', '\xCB', '\x6A', '\x56', '\xF1', '\xFA', '\x77',
'\x93', '\x41', '\x64', '\x19', '\x54', '\xA9', '\x5B', '\xC4', '\xF3', '\xF9', '\x01', '\x94', '\xA5', '\x2D',
'\x71', '\x98', '\x7A', '\x8A', '\x55', '\xA0', '\x39', '\x24', '\x0F', '\xB7', '\x1C', '\x39', '\xC7', '\x6A',
'\x62', '\x27', '\xDE', '\xFE', '\xA3', '\xB5', '\x2E', '\x5B', '\x6D', '\x94', '\x0E', '\x8A', '\xC3', '\xC5',
'\xD1', '\xF1', '\x12', '\x5F', '\xDF', '\x74', '\x41', '\x36', '\x11', '\x89', '\x4C', '\xCE', '\x83', '\x46',
'\x40', '\x8A', '\x00', '\x09', '\xE4', '\xE2', '\xEF', '\x09', '\xB9', '\x47', '\xA6', '\xB5', '\xD2', '\xAD',
'\x6A', '\x15', '\xE2', '\x78', '\x79', '\x92', '\x9C', '\xA6', '\x67', '\xD1', '\x0F', '\x72', '\x0E', '\xE3',
'\xE8', '\x26', '\x01', '\x00', '\x00', '\x1F', '\x8B', '\x08', '\x04', '\x00', '\x00', '\x00', '\x00', '\x00',
'\xFF', '\x06', '\x00', '\x42', '\x43', '\x02', '\x00', '\x1B', '\x00', '\x03', '\x00', '\x00', '\x00', '\x00',
'\x00', '\x00', '\x00', '\x00', '\x00'
};
TEST_F(alignment_file_input_f, decompression_by_filename_bgzf)
{
seqan3::test::tmp_filename filename{"alignment_file_output_test.sam.bgzf"};
{
std::ofstream of{filename.get_path(), std::ios::binary};
std::copy(input_bgzf.begin(), input_bgzf.end(), std::ostreambuf_iterator<char>{of});
}
seqan3::alignment_file_input fin{filename.get_path()};
decompression_impl(*this, fin);
}
TEST_F(alignment_file_input_f, decompression_by_stream_bgzf)
{
seqan3::alignment_file_input fin{std::istringstream{input_bgzf}, seqan3::format_sam{}};
decompression_impl(*this, fin);
}
TEST_F(alignment_file_input_f, read_empty_bgzf_file)
{
std::string empty_bgzf_file
{
'\x1F', '\x8B', '\x08', '\x04', '\x00', '\x00', '\x00', '\x00', '\x00', '\xFF',
'\x06', '\x00', '\x42', '\x43', '\x02', '\x00', '\x1B', '\x00', '\x03', '\x00',
'\x00', '\x00', '\x00', '\x00', '\x00', '\x00', '\x00', '\x00'
};
seqan3::alignment_file_input fin{std::istringstream{empty_bgzf_file}, seqan3::format_sam{}};
EXPECT_TRUE(fin.begin() == fin.end());
}
#endif
#ifdef SEQAN3_HAS_BZIP2
std::string input_bz2
{
'\x42','\x5A','\x68','\x39','\x31','\x41','\x59','\x26','\x53','\x59','\x7B','\xE2','\xE1','\x92','\x00','\x00',
'\x5C','\x5F','\x80','\x00','\x30','\x2D','\xFF','\xFF','\x90','\x3C','\x83','\x0C','\x00','\x27','\x20','\x10',
'\x60','\x20','\x00','\x8A','\x86','\x82','\x4D','\x4D','\xA6','\xA6','\x9A','\x60','\xD4','\xC8','\xC9','\x99',
'\x35','\x34','\x06','\x44','\x9B','\x51','\xA0','\x83','\x4C','\x06','\x88','\x31','\x19','\x32','\xDF','\x59',
'\x81','\x84','\x10','\x62','\x4B','\x06','\x22','\x21','\xA8','\xEA','\x68','\xCD','\xA2','\x15','\xB7','\xE5',
'\xA7','\xAB','\x0A','\xD2','\xB8','\x0A','\xEF','\xC3','\x18','\x35','\xFE','\x2C','\xE9','\x1C','\x72','\x8D',
'\xA6','\xE2','\xC7','\x3D','\xBC','\x41','\x0E','\x00','\x50','\x3E','\x05','\x0E','\x0F','\x46','\xF5','\x2B',
'\x39','\xEF','\x92','\xA5','\x28','\x85','\xEA','\xA5','\x93','\xE0','\xFD','\x27','\xBF','\x76','\xCC','\xE2',
'\x6A','\xE9','\x32','\xE0','\x11','\x05','\x09','\x44','\xD2','\x51','\xB6','\x90','\x2A','\x73','\x94','\x54',
'\x62','\x96','\x19','\xBB','\x92','\xE4','\xB8','\x20','\x28','\x32','\x8E','\x0C','\x09','\xE7','\xF8','\xBB',
'\x92','\x29','\xC2','\x84','\x83','\xDF','\x17','\x0C','\x90'
};
TEST_F(alignment_file_input_f, decompression_by_filename_bz2)
{
seqan3::test::tmp_filename filename{"alignment_file_output_test.sam.bz2"};
{
std::ofstream of{filename.get_path(), std::ios::binary};
std::copy(input_bz2.begin(), input_bz2.end(), std::ostreambuf_iterator<char>{of});
}
seqan3::alignment_file_input fin{filename.get_path()};
decompression_impl(*this, fin);
}
TEST_F(alignment_file_input_f, decompression_by_stream_bz2)
{
seqan3::alignment_file_input fin{std::istringstream{input_bz2}, seqan3::format_sam{}};
decompression_impl(*this, fin);
}
TEST_F(alignment_file_input_f, read_empty_bz2_file)
{
std::string empty_zipped_file
{
'\x42', '\x5a', '\x68', '\x39', '\x17', '\x72', '\x45', '\x38', '\x50', '\x90', '\x00', '\x00', '\x00', '\x00'
};
seqan3::alignment_file_input fin{std::istringstream{empty_zipped_file}, seqan3::format_sam{}};
EXPECT_TRUE(fin.begin() == fin.end());
}
#endif
// ----------------------------------------------------------------------------
// SAM format specificities
// ----------------------------------------------------------------------------
struct alignment_file_input_sam_format_f : public alignment_file_input_f
{
std::vector<seqan3::dna4_vector> const ref_seqs = {"ACTGATCGAGAGGATCTAGAGGAGATCGTAGGAC"_dna4};
std::vector<std::string> const ref_ids = {"ref"};
std::vector<seqan3::gapped<seqan3::dna4>> ref_seq_gapped1 = {'A'_dna4, 'C'_dna4, 'T'_dna4, 'G'_dna4};
std::vector<seqan3::gapped<seqan3::dna4>> ref_seq_gapped2 = {'C'_dna4, 'T'_dna4, 'G'_dna4, 'A'_dna4,
'T'_dna4, 'C'_dna4, 'G'_dna4, 'A'_dna4,
'G'_dna4};
std::vector<seqan3::gapped<seqan3::dna4>> ref_seq_gapped3 = {'T'_dna4, 'G'_dna4, 'A'_dna4, 'T'_dna4,
'C'_dna4, 'G'_dna4, 'A'_dna4, 'G'_dna4,};
std::vector<std::pair<std::vector<seqan3::gapped<seqan3::dna4>>,
std::vector<seqan3::gapped<seqan3::dna5>>>> alignments_expected
{
{ref_seq_gapped1, std::vector<seqan3::gapped<seqan3::dna5>>{'C'_dna5, seqan3::gap{}, 'G'_dna5, 'T'_dna5}},
{ref_seq_gapped2, std::vector<seqan3::gapped<seqan3::dna5>>{'A'_dna5, 'G'_dna5, 'G'_dna5, 'C'_dna5, 'T'_dna5,
'G'_dna5, 'N'_dna5, seqan3::gap{}, 'A'_dna5}},
{ref_seq_gapped3, std::vector<seqan3::gapped<seqan3::dna5>>{'G'_dna5, seqan3::gap{}, 'A'_dna5, 'G'_dna5,
'T'_dna5, 'A'_dna5, seqan3::gap{}, 'T'_dna5}}
};
};
TEST_F(alignment_file_input_sam_format_f, construct_by_filename_and_read_alignments)
{
seqan3::test::tmp_filename filename{"alignment_file_input_constructor.sam"};
{
std::ofstream filecreator{filename.get_path(), std::ios::out | std::ios::binary};
filecreator << input;
}
seqan3::alignment_file_input fin{filename.get_path(), ref_ids, ref_seqs, seqan3::fields<seqan3::field::alignment>{}};
EXPECT_EQ(fin.header().ref_ids(), ref_ids);
size_t counter = 0;
for (auto & [ alignment ] : fin)
{
EXPECT_RANGE_EQ(std::get<0>(alignment), std::get<0>(alignments_expected[counter]));
EXPECT_RANGE_EQ(std::get<0>(alignment), std::get<0>(alignments_expected[counter]));
counter++;
}
EXPECT_EQ(counter, 3u);
}
TEST_F(alignment_file_input_sam_format_f, construct_from_stream_and_read_alignments)
{
seqan3::alignment_file_input fin{std::istringstream{input},
ref_ids,
ref_seqs,
seqan3::format_sam{},
seqan3::fields<seqan3::field::alignment>{}};
EXPECT_EQ(fin.header().ref_ids(), ref_ids);
size_t counter = 0;
for (auto & [ alignment ] : fin)
{
EXPECT_RANGE_EQ(std::get<0>(alignment), std::get<0>(alignments_expected[counter]));
EXPECT_RANGE_EQ(std::get<0>(alignment), std::get<0>(alignments_expected[counter]));
counter++;
}
EXPECT_EQ(counter, 3u);
}
TEST_F(alignment_file_input_sam_format_f, construct_from_stream_and_read_alignment_with_dummy)
{
seqan3::alignment_file_input fin{std::istringstream{input},
seqan3::format_sam{},
seqan3::fields<seqan3::field::alignment>{}};
size_t counter = 0;
for (auto & [ alignment ] : fin)
{
EXPECT_EQ(std::get<1>(alignment), std::get<1>(alignments_expected[counter]));
counter++;
}
EXPECT_EQ(counter, 3u);
}
// ----------------------------------------------------------------------------
// BAM format specificities
// ----------------------------------------------------------------------------
struct alignment_file_input_bam_format_f : public alignment_file_input_sam_format_f
{
std::string binary_input{ // corresponds to 'input' from alignment_file_input_f fixture
'\x1F', '\x8B', '\x08', '\x04', '\x00', '\x00', '\x00', '\x00', '\x00', '\xFF', '\x06', '\x00', '\x42',
'\x43', '\x02', '\x00', '\x8D', '\x00', '\x73', '\x72', '\xF4', '\x65', '\x4C', '\x66', '\x60', '\x60',
'\x70', '\xF0', '\x70', '\xE1', '\x0C', '\xF3', '\xB3', '\x32', '\xD4', '\x33', '\xE3', '\x0C', '\xF6',
'\xB7', '\x2A', '\xCD', '\xCB', '\xCE', '\xCB', '\x2F', '\xCF', '\xE3', '\x74', '\xF7', '\xB7', '\xCA',
'\xCB', '\xCF', '\x4B', '\xE5', '\x72', '\x08', '\x0E', '\xE4', '\x0C', '\xF6', '\xB3', '\x2A', '\x4A',
'\x4D', '\xE3', '\xF4', '\xF1', '\xB3', '\x32', '\x36', '\xE1', '\x72', '\x08', '\x70', '\xE7', '\xF4',
'\x74', '\xB1', '\x2A', '\x28', '\xCA', '\x4F', '\x37', '\xE4', '\x0C', '\xF0', '\xB3', '\x4A', '\xCE',
'\xCF', '\xCF', '\x89', '\x07', '\xF1', '\x8A', '\x12', '\x73', '\xB9', '\x1C', '\x9C', '\xFD', '\x39',
'\x43', '\x32', '\x32', '\x8B', '\x15', '\x80', '\x28', '\x51', '\x21', '\x39', '\x3F', '\x37', '\x37',
'\x35', '\xAF', '\x44', '\x8F', '\x8B', '\x11', '\x68', '\x0D', '\x0B', '\x10', '\x03', '\x4D', '\x61',
'\x50', '\x02', '\xD2', '\x00', '\xF2', '\x5C', '\x8E', '\x8D', '\x7B', '\x00', '\x00', '\x00', '\x1F',
'\x8B', '\x08', '\x04', '\x00', '\x00', '\x00', '\x00', '\x00', '\xFF', '\x06', '\x00', '\x42', '\x43',
'\x02', '\x00', '\x9F', '\x00', '\x73', '\x61', '\x40', '\x00', '\x36', '\x5B', '\x4F', '\x21', '\x16',
'\x06', '\x4D', '\x06', '\x16', '\x28', '\x9F', '\x13', '\x88', '\x75', '\x18', '\x19', '\x18', '\x8A',
'\x52', '\x13', '\x53', '\x0C', '\x19', '\x44', '\x80', '\x3C', '\x01', '\x20', '\x16', '\x02', '\x62',
'\x05', '\x10', '\xED', '\xC1', '\xC0', '\xC4', '\xC4', '\xEC', '\x18', '\xEC', '\xCC', '\xE4', '\xE7',
'\xEB', '\xCC', '\x1E', '\x04', '\xD5', '\xC3', '\x08', '\x32', '\xC7', '\x0E', '\x64', '\x8E', '\x16',
'\x58', '\x3F', '\xBA', '\x39', '\x46', '\x0C', '\x05', '\x50', '\x33', '\x40', '\x66', '\x81', '\xCC',
'\x14', '\x71', '\x6A', '\xF9', '\xE8', '\x00', '\x32', '\x8A', '\x95', '\x8D', '\x9D', '\x83', '\xB3',
'\xA2', '\xD2', '\x29', '\x98', '\x19', '\x28', '\xCA', '\x0C', '\x74', '\x05', '\x2B', '\x83', '\x1F',
'\xD4', '\x04', '\x26', '\x90', '\xA9', '\xF6', '\x9E', '\x42', '\xEC', '\x0C', '\xDA', '\x0C', '\x1C',
'\x58', '\x4C', '\x35', '\x46', '\x71', '\x9D', '\x03', '\x9A', '\x0D', '\x2E', '\x22', '\x8D', '\x8D',
'\x40', '\xF5', '\x5C', '\xDC', '\x3C', '\xBC', '\x7C', '\x00', '\xC5', '\xFD', '\x4B', '\xCD', '\xF0',
'\x00', '\x00', '\x00', '\x1F', '\x8B', '\x08', '\x04', '\x00', '\x00', '\x00', '\x00', '\x00', '\xFF',
'\x06', '\x00', '\x42', '\x43', '\x02', '\x00', '\x1B', '\x00', '\x03', '\x00', '\x00', '\x00', '\x00',
'\x00', '\x00', '\x00', '\x00', '\x00'
};
};
#if SEQAN3_HAS_ZLIB
TEST_F(alignment_file_input_bam_format_f, construct_by_filename)
{
seqan3::test::tmp_filename filename{"alignment_file_input_constructor.bam"};
{
std::ofstream filecreator{filename.get_path(), std::ios::out | std::ios::binary};
filecreator << binary_input;
}
seqan3::alignment_file_input fin{filename.get_path(), ref_ids, ref_seqs, seqan3::fields<seqan3::field::id,
seqan3::field::seq,
seqan3::field::qual,
seqan3::field::alignment>{}};
EXPECT_EQ(fin.header().ref_ids(), ref_ids);
EXPECT_EQ(fin.header().comments[0], std::string{"This is a comment."});
size_t counter = 0;
for (auto & [ id, seq, qual, alignment ] : fin)
{
EXPECT_EQ(id, id_comp[counter]);
EXPECT_EQ(seq, seq_comp[counter]);
EXPECT_EQ(qual, qual_comp[counter]);
EXPECT_RANGE_EQ(std::get<0>(alignment), std::get<0>(alignments_expected[counter]));
EXPECT_RANGE_EQ(std::get<0>(alignment), std::get<0>(alignments_expected[counter]));
counter++;
}
EXPECT_EQ(counter, 3u);
}
TEST_F(alignment_file_input_bam_format_f, construct_by_stream)
{
std::istringstream stream{binary_input};
seqan3::alignment_file_input fin{stream,
ref_ids,
ref_seqs,
seqan3::format_bam{},
seqan3::fields<seqan3::field::id,
seqan3::field::seq,
seqan3::field::qual,
seqan3::field::alignment>{}};
EXPECT_EQ(fin.header().ref_ids(), ref_ids);
EXPECT_EQ(fin.header().comments[0], std::string{"This is a comment."});
size_t counter = 0;
for (auto & [ id, seq, qual, alignment ] : fin)
{
EXPECT_EQ(id, id_comp[counter]);
EXPECT_EQ(seq, seq_comp[counter]);
EXPECT_EQ(qual, qual_comp[counter]);
EXPECT_RANGE_EQ(std::get<0>(alignment), std::get<0>(alignments_expected[counter]));
EXPECT_RANGE_EQ(std::get<0>(alignment), std::get<0>(alignments_expected[counter]));
counter++;
}
EXPECT_EQ(counter, 3u);
}
#endif // SEQAN3_HAS_ZLIB
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