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Categories Features seqkit fasta_utilities fastx_toolkit pyfaidx seqmagick seqtk
**Formats supports** Multi-line FASTA Yes Yes -- Yes Yes Yes
FASTQ Yes Yes Yes -- Yes Yes
Multi-line FASTQ Yes Yes -- -- Yes Yes
Validating sequences Yes -- Yes Yes -- --
Supporting RNA Yes Yes -- -- Yes Yes
**Functions** Searching by motifs Yes Yes -- -- Yes --
Sampling Yes -- -- -- Yes Yes
Extracting sub-sequence Yes Yes -- Yes Yes Yes
Removing duplicates Yes -- -- -- Partly --
Splitting Yes Yes -- Partly -- --
Splitting by seq Yes -- Yes Yes -- --
Shuffling Yes -- -- -- -- --
Sorting Yes Yes -- -- Yes --
Locating motifs Yes -- -- -- -- --
Common sequences Yes -- -- -- -- --
Cleaning bases Yes Yes Yes Yes -- --
Transcription Yes Yes Yes Yes Yes Yes
Translation -- Yes Yes Yes Yes --
Filtering by size Indirect Yes -- Yes Yes --
Renaming header Yes Yes -- -- Yes Yes
**Other features** Cross-platform Yes Partly Partly Yes Yes Yes
Reading STDIN Yes Yes Yes -- Yes Yes
Reading gzipped file Yes Yes -- -- Yes Yes
Writing gzip file Yes -- -- -- Yes --
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