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/* File: detailview.c
* Author: Gemma Barson, 2009-11-23
* Copyright (c) 2009 - 2012 Genome Research Ltd
* ---------------------------------------------------------------------------
* SeqTools is free software; you can redistribute it and/or
* modify it under the terms of the GNU General Public License
* as published by the Free Software Foundation; either version 3
* of the License, or (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program; if not, write to the Free Software
* Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA 02111-1307, USA.
* or see the on-line version at http://www.gnu.org/copyleft/gpl.txt
* ---------------------------------------------------------------------------
* This file is part of the SeqTools sequence analysis package,
* written by
* Gemma Barson (Sanger Institute, UK) <gb10@sanger.ac.uk>
*
* based on original code by
* Erik Sonnhammer (SBC, Sweden) <Erik.Sonnhammer@sbc.su.se>
*
* and utilizing code taken from the AceDB and ZMap packages, written by
* Richard Durbin (Sanger Institute, UK) <rd@sanger.ac.uk>
* Jean Thierry-Mieg (CRBM du CNRS, France) <mieg@kaa.crbm.cnrs-mop.fr>
* Ed Griffiths (Sanger Institute, UK) <edgrif@sanger.ac.uk>
* Roy Storey (Sanger Institute, UK) <rds@sanger.ac.uk>
* Malcolm Hinsley (Sanger Institute, UK) <mh17@sanger.ac.uk>
*
* Description: See detailview.h
*----------------------------------------------------------------------------
*/
#include <blixemApp/blixem_.hpp>
#include <blixemApp/blxcontext.hpp>
#include <blixemApp/detailview.hpp>
#include <blixemApp/detailviewtree.hpp>
#include <blixemApp/blxwindow.hpp>
#include <blixemApp/bigpicture.hpp>
#include <blixemApp/exonview.hpp>
#include <seqtoolsUtils/utilities.hpp>
#include <seqtoolsUtils/blxmsp.hpp>
#include <blixemApp/coverageview.hpp>
#include <gtk/gtk.h>
#include <string.h>
#include <stdlib.h>
#include <math.h>
#include <algorithm>
#include <string>
#include <sstream>
using namespace std;
#define DETAIL_VIEW_TOOLBAR_NAME "DetailViewToolbarName"
#define DETAIL_VIEW_WIDGET_NAME "DetailViewWidget"
#define DETAIL_VIEW_FEEDBACK_REF_COORD "DetailViewFeedbackRefCoord"
#define DETAIL_VIEW_FEEDBACK_MATCH_COORD "DetailViewFeedbackMatchCoord"
#define DETAIL_VIEW_FEEDBACK_MATCH_NAME "DetailViewFeedbackMatchName"
#define DETAIL_VIEW_FEEDBACK_MATCH_LEN "DetailViewFeedbackMatchLen"
#define DETAIL_VIEW_FEEDBACK_DEPTH "DetailViewFeedbackDepth"
#define DETAIL_VIEW_FEEDBACK_DEPTH_TOOLTIP "Read depth at selected coord(s) for active strand"
#define DETAIL_VIEW_FEEDBACK_MATCH_NAME_TOOLTIP "Selected feature name"
#define DETAIL_VIEW_FEEDBACK_MIN_WIDTH 2
#define DETAIL_VIEW_FEEDBACK_MAX_WIDTH 30
#define SORT_BY_NAME_STRING "Name"
#define SORT_BY_SCORE_STRING "Score"
#define SORT_BY_ID_STRING "Identity"
#define SORT_BY_POS_STRING "Position"
#define SORT_BY_GROUP_ORDER_STRING "Group"
#define FONT_INCREMENT_SIZE 1
#define NO_SUBJECT_SELECTED_TEXT "<no subject selected>"
#define MULTIPLE_SUBJECTS_SELECTED_TEXT "<multiple subjects selected>"
#define MULTIPLE_VARIATIONS_TEXT "<multiple variations>"
#define MULTIPLE_POLYA_SIGNALS_TEXT "<multiple polyA signals>"
#define MULTIPLE_POLYA_SITES_TEXT "<multiple polyA sites>"
#define DEFAULT_SNP_CONNECTOR_HEIGHT 0
#define DEFAULT_NUM_UNALIGNED_BASES 5 /* the default number of additional bases to show if displaying unaligned parts of the match sequence */
#define POLYA_SIG_BASES_UPSTREAM 50 /* the number of bases upstream from a polyA tail to search for polyA signals */
#define POLYA_SIGNAL "aataaa"
#define SETTING_NAME_NUM_UNALIGNED_BASES "num-unaligned-bases"
typedef struct
{
const int startDnaIdx; /* the DNA coord to start searching from */
const gboolean searchRight; /* search towards the right or left */
const int searchDirection; /* multiplier to add/subtract depending on whether searching right/left */
const gboolean displayRev; /* true if the display is reversed */
const BlxSeqType seqType; /* whether viewing DNA or peptide seqs */
const int numFrames; /* number of reading frames */
const IntRange* const refSeqRange; /* the full range of the reference sequence */
GList *seqList; /* only search matches in these sequences */
int offset; /* the offset of the found MSP */
int foundFrame; /* which ref seq frame the MSP we chose is in */
MSP *foundMsp; /* the found msp */
} MatchSearchData;
/* We need to store some info about the paned window for the SNP track so that we can cache and
* reinstate the splitter position when the user toggles the display off and on again. */
class PanedWindowProperties
{
public:
GtkWidget *widget; /* the paned window */
int splitterPos; /* -1 for unset. If >=0 then it is a cached position ready for re-instating */
};
/* Utility struct to pass data to a recursive function */
typedef struct _RecursiveFuncData
{
const char *widgetName; /* the name of widgets that the function will be called on */
GtkCallback callbackFunc; /* the function to call */
gpointer callbackData; /* user data to pass to the function */
} RecursiveFuncData;
/* Local function declarations */
static BlxContext* detailViewGetContext(GtkWidget *detailView);
static GtkWidget* detailViewGetBigPicture(GtkWidget *detailView);
static GtkWidget* detailViewGetFeedbackBox(GtkWidget *detailView);
static int detailViewGetSnpConnectorHeight(GtkWidget *detailView);
static void refreshDetailViewHeaders(GtkWidget *detailView);
static void panedWindowCreateProperties(GtkWidget *widget, const int splitterPos);
static PanedWindowProperties* panedWindowGetProperties(GtkWidget *widget);
static void onDestroyPanedWindow(GtkWidget *widget);
static void snpTrackSetStrand(GtkWidget *snpTrack, const BlxStrand strand);
static BlxStrand snpTrackGetStrand(GtkWidget *snpTrack, GtkWidget *detailView);
static void snpTrackSetHeight(GtkWidget *detailView, GtkWidget *snpTrack);
static void getVariationDisplayRange(const MSP *msp, const gboolean expand, const BlxSeqType seqType, const int numFrames, const gboolean displayRev, const int activeFrame, const IntRange* const refSeqRange, IntRange *displayRange, IntRange *expandedRange);
static void recalculateSnpTrackBorders(GtkWidget *snpTrack, gpointer data);
static gboolean widgetIsTree(GtkWidget *widget);
static gboolean widgetIsTreeContainer(GtkWidget *widget);
static void updateCellRendererFont(GtkWidget *detailView, PangoFontDescription *fontDesc);
static void setDetailViewScrollPos(GtkAdjustment *adjustment, int value);
static const char* spliceSiteGetBases(const BlxSpliceSite *spliceSite, const gboolean donor, const gboolean reverse, const gboolean revcomp);
static int getNumSnpTrackRows(const BlxContext *bc, DetailViewProperties *properties, const BlxStrand strand, const int frame);
static int getVariationRowNumber(const IntRange* const rangeIn, const int numRows, GSList **rows);
static void freeRowsList(GSList *rows);
static gboolean coordAffectedByVariation(const int dnaIdx,
const BlxStrand strand,
BlxContext *bc,
const MSP **msp,
gboolean *drawStartBoundary,
gboolean *drawEndBoundary,
gboolean *drawJoiningLines,
gboolean *drawBackground,
gboolean *multipleVariations);
static gboolean coordAffectedByPolyASignal(const int dnaIdx,
const BlxStrand strand,
BlxContext *bc,
const MSP **mspOut,
gboolean *multipleVariations);
static gboolean coordAffectedByPolyASite(const int dnaIdx,
const BlxStrand strand,
BlxContext *bc,
const MSP **mspOut,
gboolean *multipleVariations);
static GtkWidget* detailViewContainerGetParentWidget(GtkContainer *container, GtkWidget *search_child, const char *parent_name);
static gboolean detailViewContainerIsParent(GtkContainer *container, GtkWidget *search_child);
static void detailViewRefreshSelection(GtkWidget *detailView);
static void setDetailViewIndex(DetailViewIndex *index,
const gboolean isSet,
const int dnaIdx,
const int displayIdx,
const int frame,
const int baseNum);
static void addBlxSpliceSite(GSList **spliceSites, const char *donorSite, const char *acceptorSite, const gboolean bothReqd) ;
static void destroyBlxSpliceSite(gpointer listItemData, gpointer data);
/***********************************************************
* Class member functions *
***********************************************************/
DetailViewProperties::DetailViewProperties(GtkWidget *detailView_in,
GtkWidget *blxWindow_in,
BlxContext *bc_in,
CoverageViewProperties *coverageViewP_in,
GtkCellRenderer *renderer_in,
GList *fwdStrandTrees_in,
GList *revStrandTrees_in,
GtkWidget *feedbackBox_in,
GtkWidget *statusBar_in,
GList *columnList_in,
GtkAdjustment *adjustment_in,
const int startCoord_in,
const BlxColumnId sortColumn_in) :
BlxPanel(detailView_in, blxWindow_in, bc_in, coverageViewP_in, 0)
{
/* Find a fixed-width font */
const char *fontFamily = findFixedWidthFont(detailView_in);
PangoFontDescription *fontDesc_in = pango_font_description_from_string(fontFamily);
pango_font_description_set_size(fontDesc_in, pango_font_description_get_size(detailView_in->style->font_desc));
renderer = renderer_in;
fwdStrandTrees = fwdStrandTrees_in;
revStrandTrees = revStrandTrees_in;
feedbackBox = feedbackBox_in;
statusBar = statusBar_in;
adjustment = adjustment_in;
fontDesc = fontDesc_in;
snpConnectorHeight = DEFAULT_SNP_CONNECTOR_HEIGHT;
numUnalignedBases = DEFAULT_NUM_UNALIGNED_BASES;
selectedIndex = NULL;
setDetailViewIndex(&selectedRangeInit, FALSE, UNSET_INT, UNSET_INT, UNSET_INT, UNSET_INT);
setDetailViewIndex(&selectedRangeStart, FALSE, UNSET_INT, UNSET_INT, UNSET_INT, UNSET_INT);
setDetailViewIndex(&selectedRangeEnd, FALSE, UNSET_INT, UNSET_INT, UNSET_INT, UNSET_INT);
/* The numunalignedbases may be set in the config file; if so, override the default */
GKeyFile *key_file = blxGetConfig();
if (key_file)
{
GError *error = NULL;
int numUnaligned = g_key_file_get_integer(key_file, SETTINGS_GROUP, SETTING_NAME_NUM_UNALIGNED_BASES, &error);
if (!error) /* we don't care if it wasn't found */
numUnalignedBases = numUnaligned;
}
/* Add the splice sites that we want Blixem to identify as canonical */
spliceSites = NULL;
addBlxSpliceSite(&spliceSites, "GT", "AG", FALSE);
addBlxSpliceSite(&spliceSites, "GC", "AG", FALSE);
addBlxSpliceSite(&spliceSites, "AT", "AC", TRUE);
/* We don't know the display range yet, so set an arbitrary range centred
* on the start coord. Set the adjustment value to be unset so that we know
* we need to calculated it first time round. */
adjustment->value = UNSET_INT;
displayRange.set(startCoord_in - 1, startCoord_in + 1);
/* Find the padding between the background area of the tree cells and the actual
* drawing area. This is used to render the full height of the background area, so
* that we don't have gaps between rows. */
if (fwdStrandTrees)
{
//GtkWidget *widget = GTK_WIDGET(fwdStrandTrees->data);
//GtkWidget *tree = widgetIsTree(widget) ? widget : treeContainerGetTree(GTK_CONTAINER(widget));
//gtk_widget_realize(tree); /* must realize tree to pick up any overriden style properties */
/* I can't get this to work properly using gtk_tree_view_get_cell_area etc. The cell
* area and background area come out wrong - perhaps because I'm not using a real
* row path. After a bit of experimentation it seems that the y padding is always
* related to the vertical-separator as follows: ypad = trunc(vseparator / 2) + 1 */
gint vertical_separator = 0, horizontal_separator = 0;
//gtk_widget_style_get (tree, "vertical-separator", &vertical_separator, NULL);
//gtk_widget_style_get (tree, "horizontal-separator", &horizontal_separator, NULL);
cellXPadding = (horizontal_separator / 2) + 1;
cellYPadding = (vertical_separator / 2) + 1;
}
exonBoundaryLineWidth = 1;
exonBoundaryLineStyle = GDK_LINE_SOLID;
exonBoundaryLineStylePartial = GDK_LINE_ON_OFF_DASH;
/* Allocate sortColumns array to be same length as columnList */
const int numColumns = g_list_length(columnList_in);
sortColumns = new BlxColumnId[numColumns];
int i = 0;
for ( ; i < numColumns; ++i)
sortColumns[i] = BLXCOL_NONE;
/* Sort by the default/input column, and then by name and then position */
sortColumns[0] = sortColumn_in;
sortColumns[1] = BLXCOL_SEQNAME;
sortColumns[2] = BLXCOL_START;
}
DetailViewProperties::~DetailViewProperties()
{
/* N.B. Don't free the cell renderer, or it causes memory corruption. I'm not
* sure what owns it - the columns it is added to? */
if (fwdStrandTrees)
{
g_list_free(fwdStrandTrees);
fwdStrandTrees = NULL;
}
if (revStrandTrees)
{
g_list_free(revStrandTrees);
revStrandTrees = NULL;
}
if (fontDesc)
{
pango_font_description_free(fontDesc);
fontDesc = NULL;
}
if (spliceSites)
{
g_slist_foreach(spliceSites, destroyBlxSpliceSite, NULL);
g_slist_free(spliceSites);
spliceSites = NULL;
}
}
double DetailViewProperties::charWidth() const
{
return m_charWidth;
}
double DetailViewProperties::charHeight() const
{
return m_charHeight;
}
/* Get the position of the left border of the main content, i.e. the
* sequence column */
double DetailViewProperties::contentXPos() const
{
double result = 0.0;
IntRange range;
getColumnXCoords(columnList(), BLXCOL_SEQUENCE, &range);
result = (double)range.min();
return result;
}
/* Get the position of the right border of the main content, i.e. the
* sequence column */
double DetailViewProperties::contentWidth() const
{
double result = (double)getColumnWidth(columnList(), BLXCOL_SEQUENCE);
return result;
}
void DetailViewProperties::setFontSize(const double charWidth, const double charHeight)
{
m_charWidth = charWidth;
m_charHeight = charHeight;
}
/***********************************************************
* Utility functions *
***********************************************************/
/* Recursively call a given function on a given widget and its
* children if it/they have the given name. The callback function,
* callback data and widget name are passed in a RecursiveFuncData
* struct */
void callFuncOnChildren(GtkWidget *widget, gpointer data)
{
RecursiveFuncData *funcData = (RecursiveFuncData*)data;
if (stringsEqual(gtk_widget_get_name(widget), funcData->widgetName, TRUE))
funcData->callbackFunc(widget, funcData->callbackData);
if (GTK_IS_CONTAINER(widget))
gtk_container_foreach(GTK_CONTAINER(widget), callFuncOnChildren, data);
}
/* Refresh headers for the detail view and its children */
void detailViewRefreshAllHeaders(GtkWidget *detailView)
{
DEBUG_ENTER("detailViewRefreshAllHeaders()");
refreshDetailViewHeaders(detailView);
callFuncOnAllDetailViewTrees(detailView, refreshTreeHeaders, NULL);
RecursiveFuncData data = {SNP_TRACK_HEADER_NAME, recalculateSnpTrackBorders, detailView};
callFuncOnChildren(detailView, &data);
gtk_widget_queue_draw(detailView);
DEBUG_EXIT("detailViewRefreshAllHeaders returning ");
}
void detailViewRedrawAll(GtkWidget *detailView)
{
DEBUG_ENTER("detailViewRedrawAll()");
/* Redraw all the trees */
callFuncOnAllDetailViewTrees(detailView, widgetClearCachedDrawable, NULL);
/* Recalculate the size of the snp track headers */
RecursiveFuncData data = {SNP_TRACK_HEADER_NAME, recalculateSnpTrackBorders, detailView};
callFuncOnChildren(detailView, &data);
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties && properties->coverageViewProperties())
properties->coverageViewProperties()->redraw();
gtk_widget_queue_draw(detailView);
DEBUG_EXIT("detailViewRedrawAll returning ");
}
/* Save any user-settings that are stored in the detail-view properties */
void detailViewSaveProperties(GtkWidget *detailView, GKeyFile *key_file)
{
DEBUG_ENTER("detailViewSaveProperties()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
g_key_file_set_integer(key_file, SETTINGS_GROUP, SETTING_NAME_NUM_UNALIGNED_BASES, properties->numUnalignedBases);
saveColumnWidths(detailViewGetColumnList(detailView), key_file);
saveSummaryColumns(detailViewGetColumnList(detailView), key_file);
DEBUG_EXIT("detailViewSaveProperties returning ");
}
/* Scroll the detail view so that the given coord is at the start of the display
* range (within bounds). the coord should be in terms of display coords */
void setDetailViewStartIdx(GtkWidget *detailView, int coord, const BlxSeqType coordSeqType)
{
DEBUG_ENTER("setDetailViewStartIdx()");
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
setDetailViewScrollPos(adjustment, coord);
DEBUG_EXIT("setDetailViewStartIdx returning ");
}
/* Scroll the detail view so that the given coord is at the end of the display
* range (within bounds). the coord should be in terms of display coords */
void setDetailViewEndIdx(GtkWidget *detailView, int coord, const BlxSeqType coordSeqType)
{
DEBUG_ENTER("setDetailViewEndIdx()");
/* Get the new start coord */
const IntRange* const displayRange = detailViewGetDisplayRange(detailView);
const int displayLen = displayRange->length();
int newStart = coord - displayLen + 1;
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
setDetailViewScrollPos(adjustment, newStart);
DEBUG_EXIT("setDetailViewEndIdx returning ");
}
/* Update the scroll position of the adjustment to the given value. Does bounds checking. */
static void setDetailViewScrollPos(GtkAdjustment *adjustment, int value)
{
DEBUG_ENTER("setDetailViewScrollPos()");
/* bounds checking */
int maxValue = adjustment->upper - adjustment->page_size + 1;
if (value > maxValue)
{
value = maxValue;
}
if (value < adjustment->lower)
{
value = adjustment->lower;
}
adjustment->value = value;
/* Emit notification that the scroll pos has changed */
gtk_adjustment_value_changed(adjustment);
DEBUG_EXIT("setDetailViewScrollPos returning ");
}
/* Scroll left/right by one base. */
void scrollDetailViewLeft1(GtkWidget *detailView)
{
DEBUG_ENTER("scrollDetailViewLeft1()");
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
setDetailViewScrollPos(adjustment, adjustment->value - 1);
DEBUG_EXIT("scrollDetailViewLeft1 returning ");
}
void scrollDetailViewRight1(GtkWidget *detailView)
{
DEBUG_ENTER("scrollDetailViewRight1()");
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
setDetailViewScrollPos(adjustment, adjustment->value + 1);
DEBUG_EXIT("scrollDetailViewRight1 returning ");
}
/* Scroll by one step increment */
void scrollDetailViewLeftStep(GtkWidget *detailView)
{
DEBUG_ENTER("scrollDetailViewLeftStep()");
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
setDetailViewScrollPos(adjustment, adjustment->value - adjustment->step_increment);
DEBUG_EXIT("scrollDetailViewLeftStep returning ");
}
void scrollDetailViewRightStep(GtkWidget *detailView)
{
DEBUG_ENTER("scrollDetailViewRightStep()");
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
setDetailViewScrollPos(adjustment, adjustment->value + adjustment->step_increment);
DEBUG_EXIT("scrollDetailViewRightStep returning ");
}
/* Scroll by one page size */
void scrollDetailViewLeftPage(GtkWidget *detailView)
{
DEBUG_ENTER("scrollDetailViewLeftPage()");
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
setDetailViewScrollPos(adjustment, adjustment->value - adjustment->page_increment);
DEBUG_EXIT("scrollDetailViewLeftPage returning ");
}
void scrollDetailViewRightPage(GtkWidget *detailView)
{
DEBUG_ENTER("scrollDetailViewRightPage()");
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
setDetailViewScrollPos(adjustment, adjustment->value + adjustment->page_increment);
DEBUG_EXIT("scrollDetailViewRightPage returning ");
}
/* Calculate the number of bases that can be displayed in the sequence column */
static int calcNumBasesInSequenceColumn(DetailViewProperties *properties)
{
DEBUG_ENTER("calcNumBasesInSequenceColumn()");
int numChars = 0;
/* Find the width of the sequence column */
int colWidth = UNSET_INT;
GList *listItem = blxWindowGetColumnList(properties->blxWindow());
for ( ; listItem; listItem = listItem->next)
{
BlxColumnInfo *columnInfo = (BlxColumnInfo*)(listItem->data);
if (columnInfo && columnInfo->columnId == BLXCOL_SEQUENCE)
{
colWidth = columnInfo->width;
break;
}
}
if (colWidth >= 0)
{
/* Don't include the cell padding area */
GtkCellRenderer *renderer = properties->renderer;
colWidth -= (2 * renderer->xpad) + (2 * renderer->xalign);
/* Return the number of whole characters that fit in the column. */
numChars = (int)((gdouble)colWidth / properties->charWidth());
}
DEBUG_EXIT("calcNumBasesInSequenceColumn returning %d", numChars);
return numChars;
}
/* This should be called when the width of the sequence column has changed (or the
* size of the font has changed). This function will adjust the scroll range of our
* custom scroll adjustment so that it represents the range that can be displayed
* in the new column width. */
void updateDetailViewRange(GtkWidget *detailView)
{
DEBUG_ENTER("updateDetailViewRange()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties->adjustment)
{
int newPageSize = calcNumBasesInSequenceColumn(properties);
/* Only trigger updates if things have actually changed */
if (newPageSize != properties->adjustment->page_size)
{
properties->adjustment->page_size = newPageSize;
properties->adjustment->page_increment = newPageSize;
///* Reset the display range so that it is between the scrollbar min and max. Try to keep
// * it centred on the same base. The base index is in terms of the display range coords,
// * so the sequence type of the coord is whatever the display sequence type is. */
// const IntRange const *displayRange = &properties->displayRange;
//
// /* First time through, both coords are set to the initial start coord. So, if
// * they are the same, just use that coord as the start coord */
// int newStart = displayRange->min;
//
// if (displayRange->min != displayRange->max)
// {
// int centre = displayRange->centre();
// int offset = roundNearest((double)properties->adjustment->page_size / 2.0);
// newStart = centre - offset;
// }
//
// const BlxSeqType seqType = blxWindowGetSeqType(properties->blxWindow());
// setDetailViewStartIdx(detailView, newStart, seqType);
gtk_adjustment_changed(properties->adjustment); /* signal that the scroll range has changed */
}
}
DEBUG_EXIT("updateDetailViewRange returning ");
}
/* Find a child widget of this widget that is a paned widget. Returns null if it does not
* have one. If there are two, it returns the first one it finds. */
static GtkWidget *findNestedPanedWidget(GtkContainer *parentWidget)
{
DEBUG_ENTER("findNestedPanedWidget()");
GtkWidget *result = NULL;
GList *children = gtk_container_get_children(parentWidget);
GList *child = children;
while (child)
{
GtkWidget *childWidget = GTK_WIDGET(child->data);
if (GTK_IS_PANED(childWidget))
{
result = childWidget;
break;
}
child = child->next;
}
g_list_free(children);
DEBUG_EXIT("findNestedPanedWidget returning ");
return result;
}
/* Add all trees in the given list to the detail view. Note that the treeList may not contain
* actual trees, it may contain their containers (e.g. if they live in scrolled windows). 'first'
* indicates that this is the first (set of) tree(s) added. This information is used to determine
* which pane to put the tree in and/or whether the first tree in the list should have headers. */
static void addTreesToDetailViewPane(GtkPaned *panedWin,
GList *treeList,
const gboolean first)
{
DEBUG_ENTER("addTreesToDetailViewPane()");
int numTrees = g_list_length(treeList);
if (numTrees == 1)
{
/* Two panes, one tree. Use 'first' flags to decide which pane to put it in */
GtkWidget *tree1 = GTK_WIDGET(treeList->data);
if (first)
{
gtk_paned_pack1(panedWin, tree1, TRUE, TRUE);
}
else
{
gtk_paned_pack2(panedWin, tree1, TRUE, TRUE);
}
}
else if (numTrees == 2)
{
/* Two panes, two trees. Easy - put one in each. */
GtkWidget *tree1 = GTK_WIDGET(treeList->data);
GtkWidget *tree2 = GTK_WIDGET(treeList->next->data);
gtk_paned_pack1(panedWin, tree1, TRUE, TRUE);
gtk_paned_pack2(panedWin, tree2, TRUE, TRUE);
}
else if (numTrees > 2)
{
/* Two panes, three trees. Put one tree in pane 1. There should be a
* nested widget in pane 2 that we can put the remaining trees in. */
GtkWidget *tree1 = GTK_WIDGET(treeList->data);
gtk_paned_pack1(panedWin, tree1, TRUE, TRUE);
GtkWidget *nestedPanedWidget = findNestedPanedWidget(GTK_CONTAINER(panedWin));
if (nestedPanedWidget)
{
/* Create a new list containing the remaining trees and call this
* function again on the nested paned widget. */
GList *remainingTrees = NULL;
GList *nextTree = treeList->next;
while (nextTree)
{
remainingTrees = g_list_append(remainingTrees, nextTree->data);
nextTree = nextTree->next;
}
/* Pass 'first' as false to make sure we don't add any more headers */
addTreesToDetailViewPane(GTK_PANED(nestedPanedWidget), remainingTrees, FALSE);
}
}
DEBUG_EXIT("addTreesToDetailViewPane returning ");
}
/* Returns true if this widget is a detail-view-tree */
static gboolean widgetIsTree(GtkWidget *widget)
{
gboolean result = FALSE;
const char *name = gtk_widget_get_name(widget);
result = (strcmp(name, DETAIL_VIEW_TREE_NAME) == 0);
return result;
}
/* Returns true if this widget is a detail-view-tree-container (that is,
* a container that has only one child that is a detail-view-tree.) */
static gboolean widgetIsTreeContainer(GtkWidget *widget)
{
gboolean result = FALSE;
const char *name = gtk_widget_get_name(widget);
result = (strcmp(name, DETAIL_VIEW_TREE_CONTAINER_NAME) == 0);
return result;
}
/* Given a widget contains a single detail-view-tree somewhere in its child
* hierarchy, return that tree. */
static GtkWidget *treeContainerGetTree(GtkContainer *container)
{
GtkWidget *result = NULL;
GList *children = gtk_container_get_children(container);
GList *child = children;
for ( ; child && !result; child = child->next)
{
if (GTK_IS_WIDGET(child->data))
{
GtkWidget *childWidget = GTK_WIDGET(child->data);
if (widgetIsTree(childWidget))
{
result = childWidget;
}
else if (GTK_IS_CONTAINER(childWidget))
{
result = treeContainerGetTree(GTK_CONTAINER(childWidget));
}
}
}
g_list_free(children);
return result;
}
/* Returns true if the given container is a parent (of any level) of the given child */
static gboolean detailViewContainerIsParent(GtkContainer *container, GtkWidget *search_child)
{
gboolean result = FALSE;
/* Loop through all children of the given container and see if any are the search_child */
GList *check_list = gtk_container_get_children(GTK_CONTAINER(container));
GList *check_child = check_list;
for ( ; check_child && !result ; check_child = check_child->next)
{
GtkWidget *check_widget = GTK_WIDGET(check_child->data);
if (check_widget == search_child)
{
result = TRUE;
}
else if (GTK_IS_CONTAINER(check_widget))
{
/* Recurse through the current widget's children */
result = detailViewContainerIsParent(GTK_CONTAINER(check_widget), search_child);
}
}
g_list_free(check_list);
return result;
}
/* Find the parent of the given child widget, where the parent has the given name.
* The parent and widget must both be in the given container */
static GtkWidget *detailViewContainerGetParentWidget(GtkContainer *container, GtkWidget *search_child, const char *parent_name)
{
GtkWidget *result = NULL;
/* Loop through all children in the container looking for a widget with parent_name */
GList *container_children = gtk_container_get_children(container);
GList *cur_item = container_children;
for ( ; cur_item && !result; cur_item = cur_item->next)
{
if (GTK_IS_WIDGET(cur_item->data))
{
GtkWidget *cur_widget = GTK_WIDGET(cur_item->data);
/* If the name matches, then check if this widget is a container with a child
* (or child-of-a-child) that matches search_child. If not, keep on searching. */
if (strcmp(gtk_widget_get_name(cur_widget), parent_name) == 0 && GTK_IS_CONTAINER(cur_widget))
{
if (detailViewContainerIsParent(GTK_CONTAINER(cur_widget), search_child))
{
result = cur_widget;
break;
}
}
if (GTK_IS_CONTAINER(cur_widget))
{
result = detailViewContainerGetParentWidget(GTK_CONTAINER(cur_widget), search_child, parent_name);
}
}
}
g_list_free(container_children);
return result;
}
/* This function removes all detail-view-trees from the given container widget. */
static void removeAllTreesFromContainer(GtkWidget *widget, gpointer data)
{
DEBUG_ENTER("removeAllTreesFromContainer()");
/* See if this widget is a tree (or a container containing only a single tree - the
* detail view contains the outermost single-tree container, e.g. typically the trees
* will live in a vbox or scrolled window, and it is this container that the detail view
* knows about) */
if (widgetIsTree(widget) || widgetIsTreeContainer(widget))
{
GtkContainer *parent = GTK_CONTAINER(data);
gtk_container_remove(parent, widget);
}
else if (GTK_IS_CONTAINER(widget))
{
/* It is a nested container, containing multiple children. Recurse. */
gtk_container_foreach(GTK_CONTAINER(widget), removeAllTreesFromContainer, widget);
}
DEBUG_EXIT("removeAllTreesFromContainer returning ");
}
/* This function removes the trees from the detail view and re-adds them in the
* correct order according to the displayRev flag. It should be called every
* time the strands are toggled. It assumes the trees are already in the
* detailView container, and that the properties have been set for all 3 widgets. */
static void refreshTreeOrder(GtkWidget *detailView)
{
DEBUG_ENTER("refreshTreeOrder()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
BlxContext *bc = detailViewGetContext(detailView);
gboolean toggled = detailViewGetDisplayRev(detailView);
/* Get the direct parent of the trees */
GtkWidget *detailViewContainer = getNamedChildWidget(detailView, DETAIL_VIEW_WIDGET_NAME);
gtk_container_foreach(GTK_CONTAINER(detailView), removeAllTreesFromContainer, detailViewContainer);
/* Extract the named detail-view widget, which is the direct parent of the trees. This
* should be a paned window. */
GtkWidget *panedWin = getNamedChildWidget(detailView, DETAIL_VIEW_WIDGET_NAME);
if (!GTK_IS_PANED(panedWin))
{
g_error("Unexpected detail view type: expected a paned widget. Could not add child trees.\n");
}
if (bc->seqType == BLXSEQ_DNA)
{
/* Add both trees - the order they're added will depend on whether the display is toggled or not. */
addTreesToDetailViewPane(GTK_PANED(panedWin), properties->fwdStrandTrees, !toggled);
addTreesToDetailViewPane(GTK_PANED(panedWin), properties->revStrandTrees, toggled);
}
else if (bc->seqType == BLXSEQ_PEPTIDE)
{
/* Only add one set of trees - the reverse strand if toggled, the forward strand if not. */
GList *treeList = toggled ? properties->revStrandTrees : properties->fwdStrandTrees;
addTreesToDetailViewPane(GTK_PANED(panedWin), treeList, TRUE);
}
/* Must show all child widgets because some of them may not have been in this parent before.
* (Just calling gtk_widget_show on the individual trees doesn't seem to work.)
* However, we then need to re-hide any that may have been previously hidden by the user. */
gtk_widget_show_all(detailView);
gtk_container_foreach(GTK_CONTAINER(detailView), hideUserHiddenWidget, NULL);
DEBUG_EXIT("refreshTreeOrder returning ");
}
/* Refresh a header that contains fixed-width text that needs to be resized whenever a zoom
* happens; updates the height of the widget and the font description, and clears its cached
* drawable, if it has one. This just sets things up ready for the next expose event, where
* the actual drawing will take place. */
void refreshTextHeader(GtkWidget *header, gpointer data)
{
const char *widgetName = gtk_widget_get_name(header);
if (GTK_IS_LABEL(header))
{
/* For straightforward labels, just update the font size. */
GtkWidget *detailView = GTK_WIDGET(data);
PangoFontDescription *dvFont = detailViewGetFontDesc(detailView);
int newSize = pango_font_description_get_size(dvFont);
pango_font_description_set_size(header->style->font_desc, newSize);
gtk_widget_modify_font(header, header->style->font_desc);
}
else if (!strcmp(widgetName, SNP_TRACK_HEADER_NAME) || !strcmp(widgetName, DNA_TRACK_HEADER_NAME))
{
/* Clear the cached drawable so that it gets recreated on the next expose */
widgetClearCachedDrawable(header, NULL);
/* Update the font and the widget height, in case the font-size has changed. */
GtkWidget *detailView = GTK_WIDGET(data);
gtk_widget_modify_font(header, detailViewGetFontDesc(detailView));
if (!strcmp(widgetName, SNP_TRACK_HEADER_NAME))
{
/* SNP track - must adjust its size based on the char height and number of rows visible */
snpTrackSetHeight(detailView, header);
}
else if (GTK_IS_LAYOUT(header))
{
/* Normal text header. Set the height to the character height */
const gdouble charHeight = detailViewGetCharHeight(detailView);
gtk_layout_set_size(GTK_LAYOUT(header), header->allocation.width, roundNearest(charHeight));
gtk_widget_set_size_request(header, -1, roundNearest(charHeight));
}
gtk_widget_queue_draw(header);
}
/* If this is a container, recurse over each child */
if (GTK_IS_CONTAINER(header))
{
gtk_container_foreach(GTK_CONTAINER(header), refreshTextHeader, data);
}
}
/* Refresh the headers for the detail view. NOte: does not refresh tree view
* headers; use detailViewRefreshAllHeaders if you want to do that */
static void refreshDetailViewHeaders(GtkWidget *detailView)
{
DEBUG_ENTER("refreshDetailViewHeaders()");
/* Loop through all widgets and call refreshTextHeader. This
* updates the font etc. if it is a type of widget that requires that. */
gtk_container_foreach(GTK_CONTAINER(detailView), refreshTextHeader, detailView);
/* Loop through all columns and call the individual refresh callbacks for
* each of their headers. This updates the specific information within the header. */
GList *columnList = detailViewGetColumnList(detailView);
GList *column = columnList;
for ( ; column; column = column->next)
{
BlxColumnInfo *columnInfo = (BlxColumnInfo*)column->data;
if (columnInfo)
{
if (columnInfo->headerWidget && columnInfo->refreshFunc)
{
columnInfo->refreshFunc(columnInfo->headerWidget, detailView);
}
}
else
{
g_critical("Invalid column data for detail view header; header may not be refreshed correctly.\n");
}
}
DEBUG_EXIT("refreshDetailViewHeaders returning ");
}
/* Resize the detail view header widgets. Should be called whenever a column is resized. */
void resizeDetailViewHeaders(GtkWidget *detailView)
{
DEBUG_ENTER("resizeDetailViewHeaders()");
GList *listItem = detailViewGetColumnList(detailView);
for ( ; listItem; listItem = listItem->next)
{
BlxColumnInfo *columnInfo = (BlxColumnInfo*)listItem->data;
/* For the sequence column, don't set the size request, or we won't be able
* to shrink the window. (The sequence col header will be resized dynamically.) */
if (columnInfo->columnId != BLXCOL_SEQUENCE)
{
/* For other columns, we can set the size request: they're small enough
* that we can live without the need to shrink below their sum widths. */
if (showColumn(columnInfo))
{
widgetSetHidden(columnInfo->headerWidget, FALSE);
gtk_widget_set_size_request(columnInfo->headerWidget, columnInfo->width, -1);
}
else
{
/* Zero width: we must hide the widget because it always seems to have
* some width even if we set the size request to 0 */
widgetSetHidden(columnInfo->headerWidget, TRUE);
}
}
}
DEBUG_EXIT("resizeDetailViewHeaders returning ");
}
/* Update the font description for all relevant components of the detail view */
void updateDetailViewFontDesc(GtkWidget *detailView)
{
DEBUG_ENTER("updateDetailViewFontDesc()");
PangoFontDescription *fontDesc = detailViewGetFontDesc(detailView);
gtk_widget_modify_font(detailView, fontDesc);
callFuncOnAllDetailViewTrees(detailView, (GtkCallback)gtk_widget_modify_font, fontDesc);
updateCellRendererFont(detailView, fontDesc);
refreshDetailViewHeaders(detailView);
callFuncOnAllDetailViewTrees(detailView, refreshTreeHeaders, NULL);
callFuncOnAllDetailViewTrees(detailView, treeUpdateFontSize, NULL);
detailViewRedrawAll(detailView);
DEBUG_EXIT("updateDetailViewFontDesc returning ");
}
static void incrementFontSize(GtkWidget *detailView)
{
DEBUG_ENTER("incrementFontSize()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
int newSize = (pango_font_description_get_size(properties->fontDesc) / PANGO_SCALE) + FONT_INCREMENT_SIZE;
if (newSize <= MAX_FONT_SIZE)
{
pango_font_description_set_size(properties->fontDesc, newSize * PANGO_SCALE);
updateDetailViewFontDesc(detailView);
}
DEBUG_EXIT("incrementFontSize returning ");
}
static void decrementFontSize(GtkWidget *detailView)
{
DEBUG_ENTER("decrementFontSize()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
int newSize = (pango_font_description_get_size(properties->fontDesc) / PANGO_SCALE) - FONT_INCREMENT_SIZE;
/* Note that the font needs to be big enough to cover the vertical separators and padding around the
* cell, otherwise we will end up with visible gaps between rows. */
if (newSize >= MIN_FONT_SIZE && newSize >= (detailViewGetCellYPadding(detailView) * 2))
{
pango_font_description_set_size(properties->fontDesc, newSize * PANGO_SCALE);
updateDetailViewFontDesc(detailView);
}
DEBUG_EXIT("decrementFontSize returning ");
}
/* Zoom the detail view in/out */
void zoomDetailView(GtkWidget *detailView, const gboolean zoomIn)
{
DEBUG_ENTER("zoomDetailView()");
if (zoomIn)
{
incrementFontSize(detailView);
}
else
{
decrementFontSize(detailView);
}
updateDetailViewRange(detailView);
DEBUG_EXIT("zoomDetailView returning ");
}
/* set the contents of a text entry box */
static void entrySetContents(GtkWidget *widget, const char *value)
{
if (widget && GTK_IS_ENTRY(widget))
{
gtk_entry_set_text(GTK_ENTRY(widget), value);
int len = strlen(value) + 1;
const int charWidth = 8;
if (len < DETAIL_VIEW_FEEDBACK_MIN_WIDTH)
len = DETAIL_VIEW_FEEDBACK_MIN_WIDTH;
if (len > DETAIL_VIEW_FEEDBACK_MAX_WIDTH)
{
len = DETAIL_VIEW_FEEDBACK_MAX_WIDTH;
/* For the Name box, if the name length has exceeded the box width then also set the
* name as the tooltip so that the user can easily see the full name. Otherwise, use
* the default tooltip text */
if (stringsEqual(gtk_widget_get_name(widget), DETAIL_VIEW_FEEDBACK_MATCH_NAME, TRUE))
gtk_widget_set_tooltip_text(widget, value);
}
else if (stringsEqual(gtk_widget_get_name(widget), DETAIL_VIEW_FEEDBACK_MATCH_NAME, TRUE))
{
gtk_widget_set_tooltip_text(widget, DETAIL_VIEW_FEEDBACK_MATCH_NAME_TOOLTIP);
}
gtk_widget_set_size_request(widget, len * charWidth, -1) ;
}
}
/* Clear the contents of a text entry box */
static void entryClearContents(GtkWidget *widget)
{
entrySetContents(widget, "");
}
/* Clear the contents of all widgets in the feedback box */
static void feedbackBoxClearValues(GtkWidget *feedbackBox)
{
entryClearContents(getNamedChildWidget(feedbackBox, DETAIL_VIEW_FEEDBACK_REF_COORD));
entryClearContents(getNamedChildWidget(feedbackBox, DETAIL_VIEW_FEEDBACK_MATCH_COORD));
entryClearContents(getNamedChildWidget(feedbackBox, DETAIL_VIEW_FEEDBACK_MATCH_NAME));
entryClearContents(getNamedChildWidget(feedbackBox, DETAIL_VIEW_FEEDBACK_MATCH_LEN));
entryClearContents(getNamedChildWidget(feedbackBox, DETAIL_VIEW_FEEDBACK_DEPTH));
}
/* Set the contents of the child text-entry widget with the given name to the given string value */
static void feedbackBoxSetString(GtkWidget *feedbackBox, const char *widgetName, const char *value)
{
GtkWidget *widget = getNamedChildWidget(feedbackBox, widgetName);
entrySetContents(widget, value);
}
/* Set the contents of the child text-entry widget with the given name to the given int value */
static void feedbackBoxSetInt(GtkWidget *feedbackBox, const char *widgetName, const int value)
{
GtkWidget *widget = getNamedChildWidget(feedbackBox, widgetName);
char *resultString = g_strdup_printf("%d", value);
entrySetContents(widget, resultString);
g_free(resultString);
}
/* Set the tooltip of the child text-entry widget with the given name */
static void feedbackBoxSetTooltip(GtkWidget *feedbackBox, const char *widgetName, const char* tooltip)
{
GtkWidget *widget = getNamedChildWidget(feedbackBox, widgetName);
gtk_widget_set_tooltip_text(widget, tooltip);
}
/* Simple utility to return true if we should negate coords for the display */
static gboolean negateCoords(BlxContext *bc)
{
return (bc->displayRev && bc->flags[BLXFLAG_NEGATE_COORDS]);
}
static void feedbackBoxSetRefCoord(GtkWidget *feedbackBox,
GtkWidget *detailView,
const BlxSequence *seq)
{
BlxContext *bc = detailViewGetContext(detailView);
if (detailViewGetSelectedIdxRangeSet(detailView))
{
/* A range of coordinates is selected */
IntRange *range = detailViewGetSelectedDnaIdxRange(detailView);
if (range)
{
stringstream result_ss;
result_ss << range->start(bc->displayRev, negateCoords(bc))
<< ".."
<< range->end(bc->displayRev, negateCoords(bc));
string result_str = result_ss.str();
feedbackBoxSetString(feedbackBox, DETAIL_VIEW_FEEDBACK_REF_COORD, result_str.c_str());
delete range;
}
}
else if (detailViewGetSelectedIdxSet(detailView))
{
/* A single coord is selected */
int qIdx = detailViewGetSelectedDnaIdx(detailView);
/* Negate the coord for the display, if necessary */
int coord = qIdx;
if (negateCoords(bc))
coord *= -1;
feedbackBoxSetInt(feedbackBox, DETAIL_VIEW_FEEDBACK_REF_COORD, coord);
}
}
static bool getMatchCoordForRefCoord(GtkWidget *detailView,
const BlxSequence *seq,
const int qIdx,
int &sIdx)
{
bool found = FALSE;
BlxContext *bc = detailViewGetContext(detailView);
g_return_val_if_fail(bc && seq, found);
GList *mspListItem = seq->mspList;
const int numUnalignedBases = detailViewGetNumUnalignedBases(detailView);
for ( ; mspListItem; mspListItem = mspListItem->next)
{
MSP *msp = (MSP*)(mspListItem->data);
BlxContext *bc = detailViewGetContext(detailView);
if (mspGetMatchCoord(msp, qIdx, TRUE, numUnalignedBases, bc, &sIdx))
{
found = TRUE;
break;
}
}
return found;
}
/* Get the match coord for the feedback box. Returns true and sets sIdx if found. */
static void feedbackBoxSetMatchCoord(GtkWidget *feedbackBox,
GtkWidget *detailView,
const BlxSequence *seq)
{
if (seq && seq->type != BLXSEQUENCE_VARIATION)
{
if (g_list_length(seq->mspList) > 0)
{
MSP *firstMsp = (MSP*)(seq->mspList->data);
if (mspGetMatchSeq(firstMsp))
{
const int sLen = strlen(mspGetMatchSeq(firstMsp));
feedbackBoxSetInt(feedbackBox, DETAIL_VIEW_FEEDBACK_MATCH_LEN, sLen);
}
/* If a q index/range is selected, see if there is a valid base at that coord(s)
* for any of the MSPs for the selected sequence. */
if (detailViewGetSelectedIdxRangeSet(detailView))
{
BlxContext *bc = detailViewGetContext(detailView);
IntRange *range = detailViewGetSelectedDnaIdxRange(detailView);
int start = UNSET_INT;
int end = UNSET_INT;
if (range &&
getMatchCoordForRefCoord(detailView, seq, range->start(bc->displayRev), start) &&
getMatchCoordForRefCoord(detailView, seq, range->end(bc->displayRev), end))
{
stringstream result_ss;
result_ss << start << ".." << end;
string result_str = result_ss.str();
feedbackBoxSetString(feedbackBox, DETAIL_VIEW_FEEDBACK_MATCH_COORD, result_str.c_str());
}
if (range)
delete range;
}
else if (detailViewGetSelectedIdxSet(detailView))
{
int qIdx = detailViewGetSelectedDnaIdx(detailView);
int sIdx = UNSET_INT;
if (getMatchCoordForRefCoord(detailView, seq, qIdx, sIdx))
{
feedbackBoxSetInt(feedbackBox, DETAIL_VIEW_FEEDBACK_MATCH_COORD, sIdx);
}
}
}
}
}
static void feedbackBoxSetMatchName(GtkWidget *feedbackBox,
const BlxSequence *seq,
const int numSeqsSelected)
{
string resultString("");
/* Find the sequence name text (or some default text to indicate that a sequence is not selected) */
const char *noSeqText = numSeqsSelected > 0 ? MULTIPLE_SUBJECTS_SELECTED_TEXT : NO_SUBJECT_SELECTED_TEXT;
if (seq)
{
const char *seqName = blxSequenceGetName(seq);
const char *sequence = blxSequenceGetSequence(seq);
if (seqName)
resultString += seqName;
/* For variations, also include the variation data in the name box */
if (seq->type == BLXSEQUENCE_VARIATION && sequence)
{
resultString += " : ";
resultString += sequence;
}
}
else
{
resultString += noSeqText;
}
feedbackBoxSetString(feedbackBox, DETAIL_VIEW_FEEDBACK_MATCH_NAME, resultString.c_str());
}
static void feedbackBoxSetDepth(GtkWidget *feedbackBox,
GtkWidget *detailView,
const BlxSequence *seq)
{
BlxContext *bc = detailViewGetContext(detailView);
const BlxStrand strand = bc->activeStrand();
if (detailViewGetSelectedIdxRangeSet(detailView))
{
/* A range of coordinates is selected. Sum the read depth over the range. */
IntRange *range = detailViewGetSelectedDisplayIdxRange(detailView);
if (range)
{
int depth = bc->calculateTotalDepth(range, strand);
feedbackBoxSetInt(feedbackBox, DETAIL_VIEW_FEEDBACK_DEPTH, depth);
delete range;
}
feedbackBoxSetTooltip(feedbackBox, DETAIL_VIEW_FEEDBACK_DEPTH, DETAIL_VIEW_FEEDBACK_DEPTH_TOOLTIP);
}
else if (detailViewGetSelectedIdxSet(detailView))
{
/* A single coord is selected */
const int coord = detailViewGetSelectedDisplayIdx(detailView);
const int depth = bc->getDepth(coord, NULL, strand);
feedbackBoxSetInt(feedbackBox, DETAIL_VIEW_FEEDBACK_DEPTH, depth);
if (bc->seqType == BLXSEQ_DNA)
{
/* Show the specific base support of the reads at this coord, i.e. how many a's, t's, g's
* and c's. Show this in the tooltip for now to avoid clutter (we could perhaps add this to
* the main box but only show the box if coverage is enabled, or have its own option). */
const int depth_a = bc->getDepth(coord, "a", strand);
const int depth_c = bc->getDepth(coord, "c", strand);
const int depth_g = bc->getDepth(coord, "g", strand);
const int depth_t = bc->getDepth(coord, "t", strand);
const int depth_n = bc->getDepth(coord, "n", strand);
const int depth_gaps = bc->getDepth(coord, ".", strand);
const int total_bases = depth_a + depth_c + depth_g + depth_t + depth_n + depth_gaps;
stringstream tmp_ss;
tmp_ss << DETAIL_VIEW_FEEDBACK_DEPTH_TOOLTIP;
/* Always show ACGT, even if 0, for consistency. We could change this however we like,
* e.g. it might be good to show them in descending order */
tmp_ss << "\nA: " << depth_a << "\nC: " << depth_c
<< "\nG: " << depth_g << "\nT: " << depth_t;
/* Only show N, Gaps and Unknown if they are non-zero because most of the time they're
* not relevant */
if (depth_n > 0)
tmp_ss << "\nN: " << depth_n;
if (depth_gaps > 0)
tmp_ss << "\nGaps: " << depth_gaps;
if (total_bases < depth)
tmp_ss << "\nUnknown: " << depth - total_bases;
string tmp_str = tmp_ss.str();
feedbackBoxSetTooltip(feedbackBox, DETAIL_VIEW_FEEDBACK_DEPTH, tmp_str.c_str());
}
}
else
{
feedbackBoxSetTooltip(feedbackBox, DETAIL_VIEW_FEEDBACK_DEPTH, DETAIL_VIEW_FEEDBACK_DEPTH_TOOLTIP);
}
}
/* Set the text displayed in the user feedback boxes based on the given MSPs sequence name
* (if an MSP is given), and also the currently-selected base index (if there is one). */
static void setFeedbackText(GtkWidget *detailView,
const BlxSequence *seq,
const int numSeqsSelected,
GtkWidget *feedbackBox)
{
DEBUG_ENTER("setFeedbackText()");
/* Clear existing values */
feedbackBoxClearValues(feedbackBox);
feedbackBoxSetRefCoord(feedbackBox, detailView, seq) ;
feedbackBoxSetMatchName(feedbackBox, seq, numSeqsSelected) ;
feedbackBoxSetMatchCoord(feedbackBox, detailView, seq) ; // also sets match length
feedbackBoxSetDepth(feedbackBox, detailView, seq) ;
DEBUG_EXIT("setFeedbackText returning ");
}
/* Updates the feedback box with info about any currently-selected MSPs. This
* currently assumes single-MSP selections, but could be extended in the future
* to display, say, summary information about multiple MSPs. */
void updateFeedbackBox(GtkWidget *detailView)
{
DEBUG_ENTER("updateFeedbackBox()");
BlxContext *bc = detailViewGetContext(detailView);
const int numSeqsSelected = g_list_length(bc->selectedSeqs);
const BlxSequence *seq = NULL;
/* currently we only properly handle single sequence selection so only pass the sequence if 1
* and only 1 sequence is selected */
if (numSeqsSelected == 1)
{
seq = (const BlxSequence*)(bc->selectedSeqs->data);
}
GtkWidget *feedbackBox = detailViewGetFeedbackBox(detailView);
setFeedbackText(detailView, seq, numSeqsSelected, feedbackBox);
gtk_widget_queue_draw(feedbackBox);
DEBUG_EXIT("updateFeedbackBox returning ");
}
/* Clear the feedback area */
void clearFeedbackArea(GtkWidget *detailView)
{
DEBUG_ENTER("clearFeedbackArea()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties)
{
GtkStatusbar *statusBar = GTK_STATUSBAR(properties->statusBar);
if (statusBar)
{
guint contextId = gtk_statusbar_get_context_id(GTK_STATUSBAR(statusBar), DETAIL_VIEW_STATUSBAR_CONTEXT);
gtk_statusbar_push(GTK_STATUSBAR(statusBar), contextId, "");
}
}
DEBUG_EXIT("clearFeedbackArea returning ");
}
/* This updates the detail-view feedback area with info about a given nucleotide in the reference sequence */
void updateFeedbackAreaNucleotide(GtkWidget *detailView, const int dnaIdx, const BlxStrand strand)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
BlxContext *bc = blxWindowGetContext(properties->blxWindow());
/* First clear the existing message if there is one */
GtkStatusbar *statusBar = GTK_STATUSBAR(properties->statusBar);
if (statusBar)
{
guint contextId = gtk_statusbar_get_context_id(GTK_STATUSBAR(statusBar), DETAIL_VIEW_STATUSBAR_CONTEXT);
gtk_statusbar_pop(GTK_STATUSBAR(statusBar), contextId);
/* See if there's a variation at the given coord */
const MSP *msp = NULL;
gboolean multiple = FALSE;
if (coordAffectedByVariation(dnaIdx, strand, bc, &msp, NULL, NULL, NULL, NULL, &multiple))
{
if (msp && mspGetSName(msp))
{
char *displayText = NULL;
/* If we're displaying coords negated, negate it now */
int coord = (negateCoords(bc) ? -1 * dnaIdx : dnaIdx);
/* If there are multiple variations on this coord, display some summary text.
* Otherwise, check we've got the sequence info to display. We should have, but
* if not just display the name. */
if (multiple)
displayText = g_strdup_printf("%d %s", coord, MULTIPLE_VARIATIONS_TEXT);
else if (mspGetMatchSeq(msp))
displayText = g_strdup_printf("%d %s : %s", coord, mspGetSName(msp), mspGetMatchSeq(msp));
else
displayText = g_strdup_printf("%d %s", coord, mspGetSName(msp));
/* Send the message to the status bar */
gtk_statusbar_push(GTK_STATUSBAR(statusBar), contextId, displayText);
g_free(displayText);
}
}
else if (coordAffectedByPolyASignal(dnaIdx, strand, bc, &msp, &multiple) && msp)
{
char *displayText = NULL;
/* If we're displaying coords negated, negate it now */
const int negate = (negateCoords(bc) ? -1 : 1);
int coord = negate * dnaIdx;
/* If there are multiple signals on this coord, display some summary text.
* Otherwise, check we've got the sequence info to display. If not just display the name. */
if (multiple)
{
displayText = g_strdup_printf("%d %s", coord, MULTIPLE_POLYA_SIGNALS_TEXT);
}
else
{
if (bc->displayRev) /* swap coords and negage if applicable */
displayText = g_strdup_printf("%d %s: %d,%d", coord, "polyA signal", negate * msp->qRange.max(), negate * msp->qRange.min());
else
displayText = g_strdup_printf("%d %s: %d,%d", coord, "polyA signal", msp->qRange.min(), msp->qRange.max());
}
/* Send the message to the status bar */
gtk_statusbar_push(GTK_STATUSBAR(statusBar), contextId, displayText);
g_free(displayText);
}
else if (coordAffectedByPolyASite(dnaIdx, strand, bc, &msp, &multiple) && msp)
{
char *displayText = NULL;
/* If we're displaying coords negated, negate it now */
const int negate = (negateCoords(bc) ? -1 : 1);
int coord = negate * dnaIdx;
/* If there are multiple sites on this coord, display some summary text.
* Otherwise, check we've got the sequence info to display. If not just display the name. */
if (multiple)
{
displayText = g_strdup_printf("%d %s", coord, MULTIPLE_POLYA_SITES_TEXT);
}
else
{
if (bc->displayRev) /* swap coords and negate if applicable */
displayText = g_strdup_printf("%d %s: %d,%d", coord, "polyA site", negate * msp->qRange.max(), negate * msp->qRange.min());
else
displayText = g_strdup_printf("%d %s: %d,%d", coord, "polyA site", msp->qRange.min(), msp->qRange.max());
}
/* Send the message to the status bar */
gtk_statusbar_push(GTK_STATUSBAR(statusBar), contextId, displayText);
g_free(displayText);
}
}
}
/* Scroll the detail-view if necessary to keep it within the given range */
void detailViewScrollToKeepInRange(GtkWidget *detailView, const IntRange* const range)
{
DEBUG_ENTER("detailViewScrollToKeepInRange()");
IntRange *displayRange = detailViewGetDisplayRange(detailView);
const BlxSeqType seqType = detailViewGetSeqType(detailView);
if (displayRange->min() < range->min())
{
setDetailViewStartIdx(detailView, range->min(), seqType);
}
else if (displayRange->max() > range->max())
{
setDetailViewEndIdx(detailView, range->max(), seqType);
}
DEBUG_EXIT("detailViewScrollToKeepInRange returning ");
}
/* If the selected base index is outside the current display range, scroll to
* keep it in range. We scroll by the minimum number of bases possible if
* scrollMinimum is true; otherwise we re-centre on the selection. */
static void scrollToKeepSelectionInRange(GtkWidget *detailView, const gboolean scrollMinimum)
{
DEBUG_ENTER("scrollToKeepSelectionInRange()");
IntRange *displayRange = detailViewGetDisplayRange(detailView);
const gboolean selectedBaseSet = detailViewGetSelectedIdxSet(detailView);
const int selectedBaseIdx = detailViewGetSelectedDisplayIdx(detailView);
if (selectedBaseSet && !valueWithinRange(selectedBaseIdx, displayRange))
{
const BlxSeqType seqType = detailViewGetSeqType(detailView);
if (scrollMinimum)
{
if (selectedBaseIdx < displayRange->min())
{
setDetailViewStartIdx(detailView, selectedBaseIdx, seqType);
}
else if (selectedBaseIdx > displayRange->max())
{
setDetailViewEndIdx(detailView, selectedBaseIdx, seqType);
}
}
else
{
const int newStart = selectedBaseIdx - (displayRange->length() / 2);
setDetailViewStartIdx(detailView, newStart, seqType);
}
}
DEBUG_EXIT("scrollToKeepSelectionInRange returning ");
}
/* This is called when the Squash matches option has been toggled. It switches to the condensed
* view of the trees (where multiple MSPs in the same sequence appear on the same row) if squash
* is true, or reverts to the expanded version (where each MSP has its own row) if squash is false. */
void detailViewUpdateSquashMatches(GtkWidget *detailView, const gboolean squash)
{
DEBUG_ENTER("detailViewUpdateSquashMatches()");
BlxContext *bc = detailViewGetContext(detailView);
if (squash && bc->modelId != BLXMODEL_SQUASHED)
{
/* Set the "squashed" model to be active */
bc->modelId = BLXMODEL_SQUASHED;
callFuncOnAllDetailViewTrees(detailView, treeUpdateSquashMatches, NULL);
}
else if (!squash && bc->modelId != BLXMODEL_NORMAL)
{
/* Set the normal model to be active */
bc->modelId = BLXMODEL_NORMAL;
callFuncOnAllDetailViewTrees(detailView, treeUpdateSquashMatches, NULL);
}
detailViewRedrawAll(detailView);
DEBUG_EXIT("detailViewUpdateSquashMatches returning ");
}
/* Sort comparison function for sorting by group */
static gint sortByGroupCompareFunc(const MSP *msp1, const MSP *msp2, GtkWidget *blxWindow)
{
gint result = 0;
/* Get the order number out of the group and sort on that. If the sequence
* is not in a group, its order number is UNSET_INT, and it gets sorted after
* any sequences that are in groups. */
const int msp1Order = sequenceGetGroupOrder(blxWindow, msp1->sSequence);
const int msp2Order = sequenceGetGroupOrder(blxWindow, msp2->sSequence);
if (msp1Order == UNSET_INT && msp2Order != UNSET_INT)
{
result = 1;
}
else if (msp1Order != UNSET_INT && msp2Order == UNSET_INT)
{
result = -1;
}
else
{
result = msp1Order - msp2Order;
}
return result;
}
/* Sort comparison function for sorting by the start position on the
* reference sequence. (Does a secondary sort by the alignment length) */
static gint sortByStartCompareFunc(const MSP *msp1, const MSP *msp2, const gboolean displayRev)
{
gint result = 0;
if (displayRev)
{
/* Display is reversed (i.e. numbers shown descending) so use compare on the max coord
* and look for the max */
result = msp2->qRange.max() - msp1->qRange.max();
}
else
{
result = msp1->qRange.min() - msp2->qRange.min();
}
if (result == 0)
{
/* If the MSPs have the same start coord, do a secondary sort
* by alignment length */
result = msp1->qRange.length() - msp2->qRange.length();
}
return result;
}
/* Sort comparison function for sorting by doubles */
static gint sortByDoubleCompareFunc(const MSP *msp1, const MSP *msp2)
{
gint result = 0;
gdouble dResult = msp1->id - msp2->id;
if (dResult == 0)
result = 0;
else if (dResult > 0)
result = 1;
else
result = -1;
return result;
}
/* Sort comparison function for sorting by the start position on the reference sequence
* when we have multiple MSPs to compare (does a secondary sort by alignment length) */
static gint sortByStartCompareFuncMultiple(GList *mspList1, GList *mspList2, const gboolean msp1Fwd, const gboolean msp2Fwd, const gboolean displayRev)
{
gint result = 0;
const int coord1 = findMspListQExtent(mspList1, !displayRev, BLXSTRAND_NONE); /* find min coord unless display rev */
const int coord2 = findMspListQExtent(mspList2, !displayRev, BLXSTRAND_NONE);
result = coord1 - coord2;
if (displayRev)
{
/* Display is reversed (i.e. numbers shown descending) so reverse the result */
result *= -1;
}
return result;
}
/* Sort comparison function for sorting by string values. Allows NULL values and
* sorts them AFTER non-null values. Comparison is case-insensitive. */
static int sortByStringCompareFunc(const char *str1, const char *str2)
{
int result = 0;
if (!str1 && !str2)
{
result = 0;
}
else if (!str1)
{
result = 1;
}
else if (!str2)
{
result = -1;
}
else
{
const int len = min(strlen(str1), strlen(str2));
result = g_ascii_strncasecmp(str1, str2, len);
}
return result;
}
/* Determine the sort order for this column. Columns may have a different default
* sort order (e.g. name is sorted ascending, but score is sorted descending.)
* However, the opposite sort order is returned if the detail view's invert-sort-
* order flag is set. */
static GtkSortType getColumnSortOrder(BlxContext *bc, const BlxColumnId columnId)
{
GtkSortType result = GTK_SORT_ASCENDING;
switch (columnId)
{
case BLXCOL_SCORE: /* fall through */
case BLXCOL_ID:
result = GTK_SORT_DESCENDING;
break;
default: /* all others ascending */
break;
};
if (bc->flags[BLXFLAG_INVERT_SORT])
{
result = (result == GTK_SORT_ASCENDING) ? GTK_SORT_DESCENDING : GTK_SORT_ASCENDING;
}
return result;
}
/* We're only interested in sorting exons, basic features and matches */
static gboolean mspIsSortable(const MSP* const msp)
{
return (msp && (typeIsMatch(msp->type) || mspIsBoxFeature(msp)));
}
/* Sort comparison function for sorting by a particular column of the tree view. */
gint sortByColumnCompareFunc(GList *mspGList1,
GList *mspGList2,
GtkWidget *detailView,
const BlxColumnId sortColumn)
{
gint result = 0;
/* Get the first MSP in each list. */
MSP *msp1 = mspGList1 ? (MSP*)(mspGList1->data) : NULL;
MSP *msp2 = mspGList2 ? (MSP*)(mspGList2->data) : NULL;
/* If an msp is of a type that we don't bother sorting, place it before any that we do sort */
if (!mspIsSortable(msp1) && !mspIsSortable(msp2))
return 0;
else if (!mspIsSortable(msp1))
return -1;
else if (!mspIsSortable(msp2))
return 1;
/* Check whether either row has more than one MSP. If so, it means some options
* aren't applicable (\to do: unless they're short reads, which should be identical if
* they're in the same row, so we can treat those as singular). */
const gboolean multipleMsps =
(g_list_length(mspGList1) > 1 || g_list_length(mspGList2) > 1);
BlxContext *bc = detailViewGetContext(detailView);
gboolean displayRev = bc->displayRev;
switch (sortColumn)
{
case BLXCOL_NONE:
result = 0;
break;
case BLXCOL_SCORE:
result = multipleMsps ? 0 : (int)(msp1->score - msp2->score);
break;
case BLXCOL_ID:
result = multipleMsps ? 0 : sortByDoubleCompareFunc(msp1, msp2);
break;
case BLXCOL_START:
if (multipleMsps)
result = sortByStartCompareFuncMultiple(mspGList1, mspGList2, msp1->qStrand == BLXSTRAND_FORWARD, msp2->qStrand == BLXSTRAND_FORWARD, displayRev);
else
result = sortByStartCompareFunc(msp1, msp2, displayRev);
break;
case BLXCOL_GROUP:
result = sortByGroupCompareFunc(msp1, msp2, detailViewGetBlxWindow(detailView));
break;
default:
/* Generic string column */
result = sortByStringCompareFunc(mspGetColumn(msp1, sortColumn), mspGetColumn(msp2, sortColumn));
break;
};
/* Invert the result if we need to sort descending instead of ascending. */
if (getColumnSortOrder(bc, sortColumn) == GTK_SORT_DESCENDING)
{
result *= -1;
}
return result;
}
/* This is the main sort comparison function for comparing two BlxSequences.
* The sort criteria are specified in the detailView properties;
* we may sort by multiple columns.
*
* Returns a negative value if the first row appears before the second,
* positive if the second appears before the first, or 0 if they are
* equivalent. */
static gint detailViewSortByColumns(gconstpointer a, gconstpointer b)
{
gint result = UNSET_INT;
const BlxSequence *seq1 = (const BlxSequence*)a;
const BlxSequence *seq2 = (const BlxSequence*)b;
GtkWidget *blxWindow = getBlixemWindow();
GtkWidget *detailView = blxWindowGetDetailView(blxWindow);
GList *columnList = detailViewGetColumnList(detailView);
const int numColumns = g_list_length(columnList);
/* Sort by each requested column in order of priority */
BlxColumnId *sortColumns = detailViewGetSortColumns(detailView);
if (sortColumns)
{
int priority = 0;
for ( ; priority < numColumns; ++priority)
{
BlxColumnId sortColumn = sortColumns[priority];
/* NONE indicates an unused entry in the priority array; if we reach
* an unset value, there should be no more values after it */
if (sortColumn == BLXCOL_NONE)
break;
/* Extract the MSPs for the two rows that we're comparing */
GList *mspGList1 = seq1->mspList;
GList *mspGList2 = seq2->mspList;
/* Do the comparison on this column */
result = sortByColumnCompareFunc(mspGList1, mspGList2, detailView, sortColumn);
/* If rows are equal, continue to sort; otherwise we're done */
if (result != 0)
break;
}
}
return result;
}
/* Re-sort all trees */
void detailViewResortTrees(GtkWidget *detailView)
{
DEBUG_ENTER("detailViewResortTrees()");
/* Sort the data for each tree */
callFuncOnAllDetailViewTrees(detailView, resortTree, NULL);
/* Sort the list of BlxSequences (uesd by the exon view) */
BlxContext *bc = detailViewGetContext(detailView);
bc->matchSeqs = g_list_sort(bc->matchSeqs, detailViewSortByColumns);
bigPictureRedrawAll(detailViewGetBigPicture(detailView));
DEBUG_EXIT("detailViewResortTrees returning ");
}
/* Set the value of the 'invert sort order' flag */
void detailViewUpdateSortInverted(GtkWidget *detailView, const gboolean invert)
{
DEBUG_ENTER("detailViewUpdateSortInverted()");
detailViewResortTrees(detailView);
DEBUG_EXIT("detailViewUpdateSortInverted returning ");
}
/* Set the value of the 'Show SNP track' flag */
void detailViewUpdateShowSnpTrack(GtkWidget *detailView, const gboolean showSnpTrack)
{
DEBUG_ENTER("detailViewUpdateShowSnpTrack()");
refreshDetailViewHeaders(detailView);
callFuncOnAllDetailViewTrees(detailView, refreshTreeHeaders, NULL);
detailViewRedrawAll(detailView);
refreshDialog(BLXDIALOG_SETTINGS, detailViewGetBlxWindow(detailView));
DEBUG_EXIT("detailViewUpdateShowSnpTrack returning ");
}
/* This performs required updates required after editing anything that affects
* the display length of MSPs in the detail-view, e.g. the 'show unaligned sequence'
* or 'show polyA tails' options. */
void detailViewUpdateMspLengths(GtkWidget *detailView, const int numUnalignedBases)
{
DEBUG_ENTER("detailViewUpdateMspLengths()");
/* Re-calculate the full extent of all MSPs, and the max msp length */
setMaxMspLen(0);
BlxContext *bc = detailViewGetContext(detailView);
MSP *msp = bc->mspList;
for ( ; msp; msp = msp->next)
{
mspCalculateFullExtents(msp, bc, numUnalignedBases);
}
/* Do a full re-sort and re-filter because the lengths of the displayed match
* sequences may have changed (and we need to make sure they're sorted by start pos) */
detailViewResortTrees(detailView);
callFuncOnAllDetailViewTrees(detailView, refilterTree, NULL);
detailViewRedrawAll(detailView);
DEBUG_EXIT("detailViewUpdateMspLengths returning ");
}
/* Set the number of additional bases to show for the show-unaligned-sequence option */
void detailViewSetNumUnalignedBases(GtkWidget *detailView, const int numBases)
{
DEBUG_ENTER("detailViewSetNumUnalignedBases()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
properties->numUnalignedBases = numBases;
detailViewUpdateMspLengths(detailView, properties->numUnalignedBases);
DEBUG_EXIT("detailViewSetNumUnalignedBases returning ");
}
int getBaseIndexAtColCoords(const int x, const int y, const gdouble charWidth, const IntRange* const displayRange)
{
int result = UNSET_INT;
const int leftEdge = 0; /* to do: allow for padding? check upper bound? */
if (x > leftEdge)
{
result = (int)(((gdouble)x - leftEdge) / charWidth);
}
result += displayRange->min();
return result;
}
/* Get the nucleotide at the given x/y position in the given sequence-column header (protein
* matches only). */
static void getSeqColHeaderClickedNucleotide(GtkWidget *header, GtkWidget *detailView, const int x, const int y, int *coordOut, int *frameOut, int *baseOut)
{
int coord = getBaseIndexAtColCoords(x, y, detailViewGetCharWidth(detailView), detailViewGetDisplayRange(detailView));
if (coord != UNSET_INT)
{
/* Get the base number of the clicked base within the active frame. The
* base number is determined by which row in the header the mouse pointer
* is over: for frame 1, row 1 will give base 1; for frame 2, row 2 will
* give base 1, etc. Start by getting the frame number for the clicked row: */
int frame = detailViewGetActiveFrame(detailView);
int row = seqColHeaderGetRow(header);
const int numFrames = detailViewGetNumFrames(detailView);
/* The header widget passed to this function is the originally-clicked widget.
* If the pointer has dragged onto another row in the header, we can work out
* which one based on the y coord. If it is outside altogether, take the
* topmost/bottommost row. (NB this assumes all rows in the header have the
* same height as this one - should be true because they're just simple labels). */
if (y < 0 || y > header->allocation.height)
{
const int offsetRows = floor((double)y / (double)header->allocation.height); /* can be negative */
row += offsetRows;
if (row < 1)
{
row = 1;
}
else if (row > numFrames)
{
row = numFrames;
}
}
/* Calculate the base number based on the row and the currently-active frame */
int baseNum = row - frame + 1;
if (baseNum < 1)
{
/* Cycle round if gone below the min base number */
baseNum += numFrames;
--coord;
}
if (coordOut)
*coordOut = coord;
if (frameOut)
*frameOut = frame;
if (baseOut)
*baseOut = baseNum;
}
}
/* Select the coord at the given x,y position in the given sequence column header */
static void selectClickedNucleotide(GtkWidget *header, GtkWidget *detailView, const int x, const int y, const gboolean extend)
{
DEBUG_ENTER("selectClickedNucleotide()");
int coord, frame, baseNum;
getSeqColHeaderClickedNucleotide(header, detailView, x, y, &coord, &frame, &baseNum);
detailViewSetSelectedDisplayIdx(detailView, coord, frame, baseNum, FALSE, TRUE, extend);
DEBUG_EXIT("selectClickedNucleotide returning ");
}
/* If the user clicked on a SNP in the SNP header, select it. This updates the
* feedback box with the SNP name and coord, selects the SNP coord and recentres
* on it (if allowScroll is true), clearing any previous selections. If expandSnps
* is true it means that the SNPs are drawn full-width (i.e. showing all the sequence
* in the MSP) and we should take into account when clicking; otherwise, the SNP
* is just shown as a marker at its actual coord, and we only need to check the
* position of that coord to see if the user clicked there. snpTrack is the widget
* that was clicked on and the optional 'colHeader' argument can be passed if coordinates
* should be converted to the coordinate system of that column header (i.e. if the SNP track
* widget is not the same width of the sequence column, the coords will need converting). */
void selectClickedSnp(GtkWidget *snpTrack,
GtkWidget *colHeader,
GtkWidget *detailView,
const int xIn,
const int yIn,
const gboolean expandSnps,
const int clickedBase)
{
DEBUG_ENTER("selectClickedSnp()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
BlxContext *bc = blxWindowGetContext(blxWindow);
const int activeFrame = detailViewGetActiveFrame(detailView);
/* Convert x coord to sequence-column coords */
int x = xIn, y = yIn;
if (colHeader)
{
gtk_widget_translate_coordinates(snpTrack, colHeader, xIn, 0, &x, NULL);
}
int clickedDisplayIdx = getBaseIndexAtColCoords(x, y, properties->charWidth(), &properties->displayRange);
/* Get the clicked row index (0-based) */
const int rowHeight = ceil(properties->charHeight());
const int clickedRow = y / rowHeight;
const int numRows = getNumSnpTrackRows(bc, properties, snpTrackGetStrand(snpTrack, detailView), activeFrame);
if (clickedDisplayIdx != UNSET_INT)
{
/* Loop through all variations and see if there are any at this displayIdx and row */
GList *snpList = NULL;
GSList *rows = NULL;
int i = 0;
const MSP *msp = mspArrayIdx(bc->featureLists[BLXMSP_VARIATION], i);
for ( ; msp; msp = mspArrayIdx(bc->featureLists[BLXMSP_VARIATION], ++i))
{
/* Get the variation coords in terms of display coords, and also the 'expanded' range
* of coords where the variation is displayed */
IntRange mspExpandedRange;
getVariationDisplayRange(msp, expandSnps, bc->seqType, bc->numFrames, bc->displayRev, activeFrame, &bc->refSeqRange, NULL, &mspExpandedRange);
const int clickedDnaIdx = convertDisplayIdxToDnaIdx(clickedDisplayIdx, bc->seqType, 1, clickedBase, bc->numFrames, bc->displayRev, &bc->refSeqRange);
gboolean found = FALSE;
int dnaIdxToSelect = UNSET_INT;
/* Get the row this variation is in */
int mspRow = 1;
if (expandSnps)
mspRow = getVariationRowNumber(&mspExpandedRange, numRows, &rows) - 1;
if (expandSnps && valueWithinRange(clickedDisplayIdx, &mspExpandedRange) && mspRow == clickedRow)
{
/* We clicked inside this MSP on the variation track. Select the first coord in
* the MSP. */
found = TRUE;
dnaIdxToSelect = msp->qRange.min();
}
else if (!expandSnps && valueWithinRange(clickedDnaIdx, &msp->qRange))
{
/* We clicked on a coord in the ref seq header that is affected by this variation.
* Select the clicked coord. */
found = TRUE;
dnaIdxToSelect = clickedDnaIdx;
}
if (found)
{
snpList = g_list_prepend(snpList, msp->sSequence);
detailViewSetSelectedDnaBaseIdx(detailView, dnaIdxToSelect, activeFrame, TRUE, FALSE, FALSE);
}
}
freeRowsList(rows);
/* Clear any existing selections and select the new SNP(s) */
blxWindowSetSelectedSeqList(blxWindow, snpList);
}
DEBUG_EXIT("selectClickedSnp returning ");
}
/***********************************************************
* Drawing *
***********************************************************/
/* Draw a vertical separator line at the edges of the given widget */
void drawColumnSeparatorLine(GtkWidget *widget,
GdkDrawable *drawable,
GdkGC *gc,
const BlxContext *bc)
{
const int lineWidth = 1; /* width of the separator lines */
if (GTK_WIDGET_VISIBLE(widget) && widget->allocation.width > lineWidth)
{
GdkColor *color = getGdkColor(BLXCOLOR_TREE_GRID_LINES, bc->defaultColors, FALSE, bc->usePrintColors);
gdk_gc_set_foreground(gc, color);
/* We want dashed lines */
gdk_gc_set_line_attributes(gc, lineWidth, GDK_LINE_ON_OFF_DASH, GDK_CAP_BUTT, GDK_JOIN_MITER);
/* Make the dashes very short and closely packed (i.e. dash length of 1 pixel and gaps of 1 pixel) */
int listLen = 1;
gint8 dashList[listLen];
dashList[0] = 1;
gdk_gc_set_dashes(gc, 0, dashList, listLen);
/* Draw vertical lines. Draw the rightmost edge of each widget (because we don't really want a
* line at the leftmost edge of the first cell of the tree view.) */
const int x = widget->allocation.x + widget->allocation.width - lineWidth;
const int y1 = widget->allocation.y;
const int y2 = y1 + widget->allocation.height;
gdk_draw_line(drawable, gc, x, y1, x, y2);
}
}
/* Populates the 'bases' string with the two bases from the ref seq adjacent to the start/end
* of the given MSP (if it is an exon/match). 'Start' here means at the lower value coord end
* of the MSP. 'revStrand' is true if this MSP is on the reverse strand of the ref seq */
static void mspGetAdjacentBases(const MSP* const msp, char *bases, const gboolean start, const gboolean revStrand, const BlxContext *bc)
{
if (start)
{
bases[0] = getSequenceIndex(bc->refSeq, msp->qRange.min() - 2, revStrand, &bc->refSeqRange, BLXSEQ_DNA);
bases[1] = getSequenceIndex(bc->refSeq, msp->qRange.min() - 1, revStrand, &bc->refSeqRange, BLXSEQ_DNA);
bases[2] = '\0';
}
else
{
bases[0] = getSequenceIndex(bc->refSeq, msp->qRange.max() + 1, revStrand, &bc->refSeqRange, BLXSEQ_DNA);
bases[1] = getSequenceIndex(bc->refSeq, msp->qRange.max() + 2, revStrand, &bc->refSeqRange, BLXSEQ_DNA);
bases[2] = '\0';
}
}
/* This function returns the prev/next MSP in the given sequence in order of start position on
* the ref seq. Returns the next MSP (with the next-highest start coord) if 'getNext' is true;
* otherwise returns the previous one. The return value will be NULL if there is no next/prev MSP.
* The error will be set if there was any problem (e.g. if the given MSP does not exist the the sequence).
* Only considers exons and matches. */
static const MSP* sequenceGetNextMsp(const MSP* const msp,
const BlxSequence *blxSeq,
const gboolean getNext,
GError **error)
{
const MSP *result = NULL;
GList *mspItem = blxSeq->mspList;
const MSP *prevMsp = NULL;
gboolean found = FALSE;
/* Loop through all MSPs and look for the given one. */
for ( ; mspItem; mspItem = mspItem->next)
{
const MSP* const curMsp = (const MSP*)(mspItem->data);
if (curMsp == msp)
{
found = TRUE;
/* If looking for the previous MSP, we already know it. */
if (!getNext)
{
result = prevMsp;
break;
}
else
{
/* Continue looping to find the next MSP. */
continue;
}
}
if (found && getNext)
{
/* We've found the input MSP and we're on the next one. See if it is an exon/match,
* otherwise continue looping. */
if (mspIsBoxFeature(curMsp) || mspIsBlastMatch(msp))
{
result = curMsp;
break;
}
}
/* Set the previous MSP (but only if it's an exon/match) */
if (mspIsBoxFeature(curMsp) || mspIsBlastMatch(curMsp))
{
prevMsp = curMsp;
}
}
if (!found && error)
{
g_set_error(error, BLX_ERROR, 1, "The given MSP '%s' was not found in the given sequence '%s'.\n", mspGetSName(msp), blxSequenceGetName(blxSeq));
}
return result;
}
/* Determine whether the bases at the other end of the intron for the start/end of
* the given MSP are canonical. 'canonicalStart' and 'canonicalEnd' contain the two bases to look
* for at the start/end of the next/previous MSP to determine if the result is canonical. */
static gboolean mspIsSpliceSiteCanonicalOtherEnd(const MSP* const msp,
const BlxSequence *blxSeq,
const gboolean isMinCoord,
const gboolean revStrand,
const gboolean donor,
const gboolean revcomp,
const BlxContext *bc,
const BlxSpliceSite *spliceSite)
{
gboolean result = FALSE;
/* Get the previous MSP if we're looking at the min coord of the current MSP, or
* the next MSP if we're at the end */
const MSP *nextMsp = sequenceGetNextMsp(msp, blxSeq, !isMinCoord, NULL);
if (nextMsp)
{
/* Get the two bases at the end of the previous MSP / start of the next MSP */
char bases[3];
mspGetAdjacentBases(nextMsp, bases, !isMinCoord, revStrand, bc);
/* If the original site was a donor site, look for an acceptor site (or vice versa) */
const char *canonicalBases = spliceSiteGetBases(spliceSite, !donor, revStrand, revcomp);
if (stringsEqual(bases, canonicalBases, FALSE))
{
result = TRUE;
}
}
return result;
}
/* Create a BlxSpliceSite and add it to the given list */
static void addBlxSpliceSite(GSList **spliceSites, const char *donorSite, const char *acceptorSite, const gboolean bothReqd)
{
BlxSpliceSite *spliceSite = new BlxSpliceSite;
if (strlen(donorSite) < 2 || strlen(acceptorSite) < 2)
{
g_critical("Error adding splice site info ['%s', '%s'].\n", donorSite, acceptorSite);
return;
}
spliceSite->donorSite[0] = donorSite[0];
spliceSite->donorSite[1] = donorSite[1];
spliceSite->donorSite[2] = '\0';
spliceSite->donorSiteComp[0] = complementChar(donorSite[0], NULL);
spliceSite->donorSiteComp[1] = complementChar(donorSite[1], NULL);
spliceSite->donorSiteComp[2] = '\0';
spliceSite->donorSiteRev[0] = donorSite[1];
spliceSite->donorSiteRev[1] = donorSite[0];
spliceSite->donorSiteRev[2] = '\0';
spliceSite->donorSiteRevComp[0] = complementChar(donorSite[1], NULL);
spliceSite->donorSiteRevComp[1] = complementChar(donorSite[0], NULL);
spliceSite->donorSiteRevComp[2] = '\0';
spliceSite->acceptorSite[0] = acceptorSite[0];
spliceSite->acceptorSite[1] = acceptorSite[1];
spliceSite->acceptorSite[2] = '\0';
spliceSite->acceptorSiteComp[0] = complementChar(acceptorSite[0], NULL);
spliceSite->acceptorSiteComp[1] = complementChar(acceptorSite[1], NULL);
spliceSite->acceptorSiteComp[2] = '\0';
spliceSite->acceptorSiteRev[0] = acceptorSite[1];
spliceSite->acceptorSiteRev[1] = acceptorSite[0];
spliceSite->acceptorSiteRev[2] = '\0';
spliceSite->acceptorSiteRevComp[0] = complementChar(acceptorSite[1], NULL);
spliceSite->acceptorSiteRevComp[1] = complementChar(acceptorSite[0], NULL);
spliceSite->acceptorSiteRevComp[2] = '\0';
spliceSite->bothReqd = bothReqd;
/* Add it to the list */
*spliceSites = g_slist_append(*spliceSites, spliceSite);
}
/* Frees the memory used by a BlxSpliceSite */
static void destroyBlxSpliceSite(gpointer listItemData, gpointer data)
{
BlxSpliceSite *spliceSite = (BlxSpliceSite*)listItemData;
delete spliceSite;
}
/* Return the canonical bases at the donor/acceptor end of the given BlxSpliceSite. Returns them in
* reverse order if 'reverse' is true, e.g. for a GC-AG intron, the dono is GC and acceptor is AG.
* If reverse is true the donor returns CG and acceptor returns GA. The result is a pointer to the
* string in the splice site, which is owned by the Detail View properties.
* If revcomp is true, then revcomp the result and swap whether it's a donor or acceptor. */
static const char* spliceSiteGetBases(const BlxSpliceSite *spliceSite,
const gboolean donor,
const gboolean reverse,
const gboolean revcomp)
{
const char *result = NULL;
if (revcomp)
{
/* Reverse the logic for everything and use complement */
if (donor)
result = reverse ? spliceSite->acceptorSiteComp : spliceSite->acceptorSiteRevComp;
else
result = reverse ? spliceSite->donorSiteComp : spliceSite->donorSiteRevComp;
}
else
{
if (donor)
result = reverse ? spliceSite->donorSiteRev : spliceSite->donorSite;
else
result = reverse ? spliceSite->acceptorSiteRev : spliceSite->acceptorSite;
}
return result;
}
/* Determines whether the intron splice sites for the given MSP are canonical or not and returns
* the relevant highlight color: green for canonical and red for non-canonical.
* We look for GT-AG, GC-AG or AT-AC introns. The latter must have both ends of the intron matching
* to be canonical. GC is the donor site and AG is the acceptor site.
* We may also return orange if a site is "maybe" canonical. This means it would be canonical if
* it were on the other strand (i.e its revcomp is canonical). This is useful for identifying
* errors in input data (common in BAM, which doesn't have comprehensive representation of all
* strand combinations). */
static BlxColorId getMspSpliceSiteColor(const MSP* const msp,
const BlxSequence *blxSeq,
const gboolean isMinCoord,
const gboolean revStrand,
const BlxContext *bc,
GSList *spliceSites)
{
BlxColorId colorId = BLXCOLOR_NON_CANONICAL;
gboolean canonical = FALSE;
gboolean maybe_canonical = FALSE;
GSList *item = spliceSites;
for ( ; item; item = item->next)
{
const BlxSpliceSite *spliceSite = (const BlxSpliceSite*)(item->data);
/* Get the two adjacent bases at the start/end of the given MSP */
char bases[3];
mspGetAdjacentBases(msp, bases, isMinCoord, revStrand, bc);
/* The min coord end of the MSP is the acceptor site of the intron and the max coord the
* donor site (or vice versa if the strand is reversed). */
const gboolean donor = (isMinCoord == revStrand);
gboolean revcomp = FALSE;
const char *canonicalBases = spliceSiteGetBases(spliceSite, donor, revStrand, revcomp);
if (stringsEqual(bases, canonicalBases, FALSE))
{
if (spliceSite->bothReqd)
{
/* Must also check if the other end of the intron matches */
if (mspIsSpliceSiteCanonicalOtherEnd(msp, blxSeq, isMinCoord, revStrand, donor, revcomp, bc, spliceSite))
{
canonical = TRUE;
break;
}
}
else
{
canonical = TRUE;
break;
}
}
else if (bc->flags[BLXFLAG_SHOW_MAYBE_CANONICAL] && !maybe_canonical) /* don't need to check if already set */
{
/* Check if the revcomp would be canonical. Toggle the donor and revStrand flags - this gets
* the expected bases as if it were the "other" splice site (i.e. donor vs acceptor)
* and reverses the order of the bases. We also need to complement them. */
revcomp = TRUE;
canonicalBases = spliceSiteGetBases(spliceSite, donor, revStrand, revcomp);
if (stringsEqual(bases, canonicalBases, FALSE))
{
if (spliceSite->bothReqd)
{
/* Must also check if the other end of the intron matches */
if (mspIsSpliceSiteCanonicalOtherEnd(msp, blxSeq, isMinCoord, revStrand, donor, revcomp, bc, spliceSite))
{
/* don't break because we need to continue to check in case it's definitely canonical */
maybe_canonical = TRUE;
}
}
else
{
/* don't break because we need to continue to check in case it's definitely canonical */
maybe_canonical = TRUE;
}
}
}
}
if (canonical)
colorId = BLXCOLOR_CANONICAL;
else if (maybe_canonical)
colorId = BLXCOLOR_MAYBE_CANONICAL;
else
colorId = BLXCOLOR_NON_CANONICAL;
return colorId;
}
/* If the given MSP is an exon/match, get the 2 nucleotides from the reference sequence at the
* splice sites of the adjacent introns. Inserts the results into the given hash table with
* an enum indicating whether the nucleotides are canonical/non-canonical. Only considers
* MSPs that are within the given ref seq range. */
static void mspGetSpliceSiteCoords(const MSP* const msp,
const BlxSequence *blxSeq,
const IntRange* const qRange,
const BlxContext *bc,
GSList *spliceSites,
GHashTable *result)
{
const gboolean revStrand = (mspGetRefStrand(msp) != BLXSTRAND_FORWARD);
/* Ignore the termini (i.e. 5' end of first exon and 3' end of last exon) */
const gboolean getMin = (msp->sSequence && msp->qRange.min() != blxSequenceGetStart(msp->sSequence, msp->qStrand));
const gboolean getMax = (msp->sSequence && msp->qRange.max() != blxSequenceGetEnd(msp->sSequence, msp->qStrand));
/* See if the min coord is within the given range */
if (getMin && valueWithinRange(msp->qRange.min(), qRange) && msp->qRange.min() >= bc->refSeqRange.min() + 2)
{
/* Find out if the adjacent bases are canonical/non-canonical. */
BlxColorId colorId = getMspSpliceSiteColor(msp, blxSeq, TRUE, revStrand, bc, spliceSites);
/* Insert the two coords into the hash table */
g_hash_table_insert(result, GINT_TO_POINTER(msp->qRange.min() - 2), GINT_TO_POINTER(colorId));
g_hash_table_insert(result, GINT_TO_POINTER(msp->qRange.min() - 1), GINT_TO_POINTER(colorId));
}
/* See if the max coord is within the given range */
if (getMax && valueWithinRange(msp->qRange.max(), qRange) && msp->qRange.max() <= bc->refSeqRange.max() - 2)
{
/* Find out if they are canonical/non-canonical */
BlxColorId colorId = getMspSpliceSiteColor(msp, blxSeq, FALSE, revStrand, bc, spliceSites);
/* Insert the two coords into the hash table */
g_hash_table_insert(result, GINT_TO_POINTER(msp->qRange.max() + 1), GINT_TO_POINTER(colorId));
g_hash_table_insert(result, GINT_TO_POINTER(msp->qRange.max() + 2), GINT_TO_POINTER(colorId));
}
}
/* This looks through all of the polyA signals and sees if any of them are in the given display
* range (in nucleotide coords) and whether we want to display them. If the 'display polyA signals for
* selected sequences only' option is enabled, then any selected sequence MSPs that have polyA tails
* should be passed in the given GSList. Any coords within a relevant polyA signal range are added to
* the given hash table with the BlxColorId that they should be drawn with. */
static void getAnnotatedPolyASignalBasesToHighlight(const BlxContext *bc,
GSList *polyATailMsps,
const BlxStrand qStrand,
const IntRange* const qRange,
GHashTable *result)
{
/* We've more work to do if showing polyA signals (unless we're only showing them for selected
* sequences and there were no relevant selected sequences) */
if (bc->flags[BLXFLAG_SHOW_POLYA_SIG] && (!bc->flags[BLXFLAG_SHOW_POLYA_SIG_SELECTED] || g_slist_length(polyATailMsps) > 0))
{
BlxColorId colorId = BLXCOLOR_POLYA_SIGNAL_ANN;
/* Loop through all polyA signals */
int i = 0;
const MSP *sigMsp = mspArrayIdx(bc->featureLists[BLXMSP_POLYA_SIGNAL], i);
for ( ; sigMsp; sigMsp = mspArrayIdx(bc->featureLists[BLXMSP_POLYA_SIGNAL], ++i))
{
/* Only interested the polyA signal has the correct strand and is within the display range. */
if (sigMsp->qStrand == qStrand && rangesOverlap(&sigMsp->qRange, qRange))
{
gboolean addSignal = FALSE;
if (bc->flags[BLXFLAG_SHOW_POLYA_SIG_SELECTED])
{
/* We're only interested in polyA signals that are 50 bases upstream of one of our polyA-tail MSPs */
GSList *item = polyATailMsps;
for ( ; item && !addSignal; item = item->next)
{
const MSP* const tailMsp = (const MSP*)(item->data);
const int qEnd = tailMsp->qRange.max();
const int qStart = qEnd - POLYA_SIG_BASES_UPSTREAM;
IntRange upstreamRange(qStart, qEnd); /* sorts out which is min and which is max */
addSignal = rangesOverlap(&sigMsp->qRange, &upstreamRange);
}
}
else
{
/* Add all signals that are in the display range */
addSignal = TRUE;
}
if (addSignal)
{
/* Add each base in the polyA signal range to the hash table. This may overwrite
* splice site bases that we previously found, which is fine (something has to take priority). */
int i = sigMsp->qRange.min();
for ( ; i <= sigMsp->qRange.max(); ++i)
{
g_hash_table_insert(result, GINT_TO_POINTER(i), GINT_TO_POINTER(colorId));
}
}
}
}
}
}
/* Scan upstream from any polyA tails to see if there are any polyA signals in the reference
* sequence. Actually, because the visible range in the detail-view is generally quite small and
* we don't want to re-scan lots of times, lets just scan through the whole visible range once
* and show all signals - but only if there is at least one visible polyA tail in the range. */
static void getPolyASignalBasesToHighlight(GtkWidget *detailView,
const BlxContext *bc,
GSList *polyATailMsps,
const IntRange* const qRange,
GHashTable *result)
{
/* If only showing polyAs for selected sequences, check that there's a (selected) msp with a
polyA tail in range (i.e. in the input list). Otherwise show all polyA signals. */
if (bc->flags[BLXFLAG_SHOW_POLYA_SIG] && (!bc->flags[BLXFLAG_SHOW_POLYA_SIG_SELECTED] || g_slist_length(polyATailMsps) > 0))
{
BlxColorId colorId = BLXCOLOR_POLYA_SIGNAL;
/* Loop through each base in the visible range */
const char *seq = bc->refSeq;
int idx = qRange->min() - bc->refSeqRange.min(); /* convert to 0-based */
const int max_idx = qRange->max() - bc->refSeqRange.min(); /* convert to 0-based */
const char *comparison = POLYA_SIGNAL;
const int comparison_len = strlen(comparison);
const char *cp = NULL;
for ( ; idx < max_idx && cp && *cp; ++idx)
{
if (idx < 0)
continue;
cp = seq + idx;
if (cp == NULL || *cp == 0)
break;
if (!strncasecmp(cp, comparison, comparison_len))
{
/* Add each base in the polyA signal range to the hash table. This may overwrite
* splice site bases that we previously found, which is fine (something has to take priority). */
int i = idx + bc->refSeqRange.min(); /* convert back to coords */
const int max = i + comparison_len;
for ( ; i < max; ++i)
{
g_hash_table_insert(result, GINT_TO_POINTER(i), GINT_TO_POINTER(colorId));
}
}
}
}
}
/* This looks through all of the polyA sites and sees if any of them are in the given display
* range (in nucleotide coords) and whether we want to display them. */
static void getAnnotatedPolyASiteBasesToHighlight(const BlxContext *bc,
const BlxStrand qStrand,
const IntRange* const qRange,
GHashTable *result)
{
/* We've more work to do if showing polyA signals (unless we're only showing them for selected
* sequences and there were no relevant selected sequences) */
if (bc->flags[BLXFLAG_SHOW_POLYA_SIG])
{
BlxColorId colorId = BLXCOLOR_POLYA_SITE_ANN;
/* Loop through all polyA signals */
int i = 0;
const MSP *siteMsp = mspArrayIdx(bc->featureLists[BLXMSP_POLYA_SITE], i);
for ( ; siteMsp; siteMsp = mspArrayIdx(bc->featureLists[BLXMSP_POLYA_SITE], ++i))
{
/* Only interested the polyA site has the correct strand and is within the display range. */
if (siteMsp->qStrand == qStrand && rangesOverlap(&siteMsp->qRange, qRange))
{
/* Add just the first base in the polyA signal range to the hash table (because the
* site is between the two bases in the range and we only want to draw it once). */
g_hash_table_insert(result, GINT_TO_POINTER(siteMsp->qRange.min()), GINT_TO_POINTER(colorId));
}
}
}
}
/* This function looks for special bases in the reference sequence header to highlight and stores their
* coords in the returned hash table with the BlxColorId (converted to a gpointer with GINT_TO_POINTER)
* of the fill color they should be drawn with. These special coords include splice sites and polyA signals. */
GHashTable* getRefSeqBasesToHighlight(GtkWidget *detailView,
const IntRange* const qRange,
const BlxSeqType seqType,
const BlxStrand qStrand)
{
GHashTable *result = g_hash_table_new(g_direct_hash, g_direct_equal);
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
const BlxContext *bc = blxWindowGetContext(blxWindow);
/* We only highlight nucleotides, so there is nothing to do if we're showing a peptide sequence,
* or if the show-splice-sites or show-polyA-signals options are disabled. */
if (seqType == BLXSEQ_PEPTIDE ||
(!bc->flags[BLXFLAG_SHOW_SPLICE_SITES] && !bc->flags[BLXFLAG_SHOW_POLYA_SIG]))
{
return result;
}
/* Loop through the selected sequences. If showing polyA signals for selected sequences, we'll
* compile a list of MSPs we're interested in seeing polyA signals for (i.e. those with polyA tails) */
DetailViewProperties *properties = detailViewGetProperties(detailView);
GList *seqItem = blxWindowGetSelectedSeqs(blxWindow);
GSList *polyATailMsps = NULL;
for ( ; seqItem; seqItem = seqItem->next)
{
const BlxSequence *blxSeq = (const BlxSequence*)(seqItem->data);
GList *mspItem = blxSeq->mspList;
/* Loop through all MSPs for this sequence */
for ( ; mspItem; mspItem = mspItem->next)
{
MSP *msp = (MSP*)(mspItem->data);
/* Only look at matches/exons on the correct strand */
if ((mspIsBlastMatch(msp) || msp->type == BLXMSP_EXON || msp->type == BLXMSP_BASIC) &&
mspGetRefStrand(msp) == qStrand)
{
if (bc->flags[BLXFLAG_SHOW_SPLICE_SITES])
mspGetSpliceSiteCoords(msp, blxSeq, qRange, bc, properties->spliceSites, result);
if (bc->flags[BLXFLAG_SHOW_POLYA_SIG] && mspHasPolyATail(msp))
polyATailMsps = g_slist_append(polyATailMsps, msp);
}
}
}
/* Now check the polyA signals and sites and see if any of them are in range. */
getPolyASignalBasesToHighlight(detailView, bc, polyATailMsps, qRange, result);
getAnnotatedPolyASignalBasesToHighlight(bc, polyATailMsps, qStrand, qRange, result);
getAnnotatedPolyASiteBasesToHighlight(bc, qStrand, qRange, result);
return result;
}
static void drawDnaTrack(GtkWidget *dnaTrack, GtkWidget *detailView, const BlxStrand strand, const int frame)
{
GdkDrawable *drawable = createBlankPixmap(dnaTrack);
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
BlxContext *bc = blxWindowGetContext(blxWindow);
/* Find the segment of the ref sequence to display (complemented if this tree is
* displaying the reverse strand, and reversed if the display is toggled). Ref seq
* coords are in nucleotides, so convert display range to nucleotide coords. */
IntRange *displayRange = detailViewGetDisplayRange(detailView);
const int qIdx1 = convertDisplayIdxToDnaIdx(displayRange->min(), bc->seqType, frame, 1, bc->numFrames, bc->displayRev, &bc->refSeqRange); /* 1st base in frame */
const int qIdx2 = convertDisplayIdxToDnaIdx(displayRange->max(), bc->seqType, frame, bc->numFrames, bc->numFrames, bc->displayRev, &bc->refSeqRange); /* last base in frame */
IntRange qRange = {min(qIdx1, qIdx2), max(qIdx1, qIdx2)};
GError *error = NULL;
gchar *segmentToDisplay = getSequenceSegment(bc->refSeq,
&qRange,
strand,
BLXSEQ_DNA, /* ref seq is always in nucleotide coords */
BLXSEQ_DNA, /* required segment is in nucleotide coords */
frame,
bc->numFrames,
&bc->refSeqRange,
bc->blastMode,
bc->geneticCode,
bc->displayRev,
bc->displayRev,
bc->displayRev,
&error);
if (!segmentToDisplay)
{
g_assert(error);
prefixError(error, "Could not draw DNA header. ");
reportAndClearIfError(&error, G_LOG_LEVEL_WARNING);
return;
}
else
{
/* If there's an error but the sequence was still returned it's a non-critical warning */
reportAndClearIfError(&error, G_LOG_LEVEL_WARNING);
}
GdkGC *gc = gdk_gc_new(drawable);
DetailViewProperties *properties = detailViewGetProperties(detailView);
const int activeFrame = detailViewGetActiveFrame(detailView);
const BlxStrand activeStrand = blxWindowGetActiveStrand(blxWindow);
const gboolean highlightSnps = bc->flags[BLXFLAG_HIGHLIGHT_VARIATIONS];
gtk_layout_set_size(GTK_LAYOUT(dnaTrack), dnaTrack->allocation.width, roundNearest(properties->charHeight()));
/* Find out if there are any bases in the introns that need highlighting. */
GHashTable *basesToHighlight = getRefSeqBasesToHighlight(detailView, &qRange, BLXSEQ_DNA, activeStrand);
/* We'll loop forward/backward through the display range depending on which strand we're viewing */
int incrementValue = bc->displayRev ? -bc->numFrames : bc->numFrames;
int displayLen = qRange.length();
char displayText[displayLen + 1];
int displayTextPos = 0;
int qIdx = bc->displayRev ? qRange.max() : qRange.min();
int displayIdx = convertDnaIdxToDisplayIdx(qIdx, bc->seqType, activeFrame, bc->numFrames, bc->displayRev, &bc->refSeqRange, NULL);
const int y = 0;
DrawBaseData baseData = {qIdx,
0,
strand,
UNSET_INT,
BLXSEQ_DNA,
TRUE,
FALSE,
FALSE,
FALSE,
highlightSnps,
TRUE,
BLXCOLOR_BACKGROUND,
NULL,
NULL,
FALSE,
FALSE,
FALSE,
FALSE,
detailViewGetSelectedDnaIdxRange(detailView)};
while (qIdx >= qRange.min() && qIdx <= qRange.max())
{
/* Get the character to display at this index, and its position */
displayText[displayTextPos] = getSequenceIndex(bc->refSeq, qIdx, bc->displayRev, &bc->refSeqRange, BLXSEQ_DNA);
const int x = (int)((gdouble)displayTextPos * properties->charWidth());
baseData.dnaIdx = qIdx;
baseData.baseChar = displayText[displayTextPos];
baseData.displayIdxSelected = detailViewIsDisplayIdxSelected(detailView, displayIdx);
baseData.dnaIdxSelected = detailViewIsDnaIdxSelected(detailView, qIdx);
/* Color the base depending on whether it is selected or affected by a SNP */
drawHeaderChar(bc, properties, drawable, gc, x, y, basesToHighlight, &baseData);
/* Increment indices */
++displayTextPos;
++displayIdx;
qIdx += incrementValue;
}
/* Make sure the string is terminated properly */
displayText[displayTextPos] = '\0';
/* Draw the text */
PangoLayout *layout = gtk_widget_create_pango_layout(detailView, displayText);
pango_layout_set_font_description(layout, detailViewGetFontDesc(detailView));
if (layout)
{
gtk_paint_layout(dnaTrack->style, drawable, GTK_STATE_NORMAL, TRUE, NULL, detailView, NULL, 0, 0, layout);
g_object_unref(layout);
}
drawColumnSeparatorLine(dnaTrack, drawable, gc, bc);
g_hash_table_unref(basesToHighlight);
g_free(segmentToDisplay);
g_object_unref(gc);
}
/* Utility to find the display coord(s) that a variation lies on. If expand is true,
* it means that if the variation contains more than one alternative, and these will be
* displayed horizontally across the display, taking up more width than where
* the actual variation coords lie. expandedRange will take this into account. displayRange
* always returns the actual msp start/end in display coords. */
static void getVariationDisplayRange(const MSP *msp,
const gboolean expand,
const BlxSeqType seqType,
const int numFrames,
const gboolean displayRev,
const int activeFrame,
const IntRange* const refSeqRange,
IntRange *displayRange,
IntRange *expandedRange)
{
/* Convert the MSP coords to display coords. We want to display the variation
* in the same position regardless of reading frame, so always use frame 1. */
const IntRange* const mspRange = mspGetDisplayRange(msp);
if (displayRange)
displayRange->set(mspRange);
if (!expandedRange)
return;
/* Work out the expanded range (it will be the same as the MSP range if unexpanded) */
expandedRange->set(mspRange);
if (expand && mspGetMatchSeq(msp))
{
/* Expand the variation range so that we display its entire sequence. We'll
* position the variation so that the middle of its sequence lies at the centre
* coord of its ref seq range */
const int numChars = strlen(mspGetMatchSeq(msp));
int offset = (int)((double)numChars / 2.0);
if (numChars % 2 == 0)
{
/* Shift to the right by one if we've got an even number of chars */
--offset;
}
expandedRange->setMin(expandedRange->centre() - offset);
expandedRange->setMax(expandedRange->min() + numChars - 1); // uses updated min
}
}
/* Utility that returns true if the given bool is either true or not
* requested (i.e. the pointer is null) */
static gboolean trueOrNotRequested(const gboolean* const ptr)
{
return (ptr == NULL || *ptr == TRUE);
}
/* Determine whether the given coord in the given frame/strand is affected by
* a variation. Sets whether to draw the start/end/top/bottom boundaries for the coord
* when outlining it by the extent of the found variation. Also sets drawBackground to true
* if the background of the base should be highlighted in the variation color (i.e. if the base
* is part of a selected variation). Sets multipleVariations to true if there is more than
* one variation at this coord */
static gboolean coordAffectedByVariation(const int dnaIdx,
const BlxStrand strand,
BlxContext *bc,
const MSP **mspOut, /* the variation we found */
gboolean *drawStartBoundary,
gboolean *drawEndBoundary,
gboolean *drawJoiningLines,
gboolean *drawBackground,
gboolean *multipleVariations)
{
gboolean result = FALSE;
/* Loop through all variations */
int i = 0;
const MSP *msp = mspArrayIdx(bc->featureLists[BLXMSP_VARIATION], i);
for ( ; msp; msp = mspArrayIdx(bc->featureLists[BLXMSP_VARIATION], ++i))
{
if (mspGetRefStrand(msp) == strand && valueWithinRange(dnaIdx, &msp->qRange))
{
/* If result has already been set, then there are multiple variations on this coord */
if (result && multipleVariations)
*multipleVariations = TRUE;
result = TRUE;
if (mspOut)
*mspOut = msp;
/* We draw the start boundary of this base if it's the first base in the variation's range
* and the end boundary if it's the last, unless it's a zero-length feature, i.e. an insertion
* site; in this case we draw a line to the right of the coord in the direction of the
* reference sequence (i.e. to give just a vertical line at the site, not an outline around the bases) */
const gboolean isFirst = (dnaIdx == msp->qRange.min());
const gboolean isLast = (dnaIdx == msp->qRange.max());
const gboolean isZeroLen = mspIsZeroLenVariation(msp);
/* Note that if any of the boundaries are already set to be drawn (by a previous snp
* found in this loop) then we keep the current value */
if (drawStartBoundary)
*drawStartBoundary |= (isZeroLen != bc->displayRev ? isLast : isFirst);
if (drawEndBoundary)
*drawEndBoundary |= (isZeroLen != bc->displayRev ? isFirst : isLast);
/* Don't draw joining lines between the start and end boundaries if it's zero-length */
if (drawJoiningLines)
*drawJoiningLines |= !isZeroLen;
/* Highlight the background if the variation is selected (unless it's a zero-length variation) */
if (drawBackground)
*drawBackground |= !isZeroLen && bc->isSeqSelected(msp->sSequence);
/* If we only want to know if this coord is affected by a snp, we can return now.
*
* If we also need the boundary-lines info for this coord, then we need to check
* all snps that are at this coord, because some may have different boundaries to
* others. We can break if all boundar-lines have already been set to true, though.
*
* If we need to return whether there are multiple snps at this coord, we can return
* after we've found the second on (i.e. after multipleVariations is set to TRUE). */
if (trueOrNotRequested(drawStartBoundary) &&
trueOrNotRequested(drawEndBoundary) &&
trueOrNotRequested(drawJoiningLines) &&
trueOrNotRequested(drawBackground) &&
trueOrNotRequested(multipleVariations))
break;
}
}
return result;
}
/* Determine whether the given coord in the given frame/strand is affected by
* a polyA signal */
static gboolean coordAffectedByPolyASignal(const int dnaIdx,
const BlxStrand strand,
BlxContext *bc,
const MSP **mspOut, /* the variation we found */
gboolean *multiple)
{
gboolean result = FALSE;
/* Loop through all variations */
int i = 0;
const MSP *msp = mspArrayIdx(bc->featureLists[BLXMSP_POLYA_SIGNAL], i);
for ( ; msp; msp = mspArrayIdx(bc->featureLists[BLXMSP_POLYA_SIGNAL], ++i))
{
if (mspGetRefStrand(msp) == strand && valueWithinRange(dnaIdx, &msp->qRange))
{
/* If result has already been set, then there are multiple signals on this coord */
if (result && multiple)
*multiple = TRUE;
result = TRUE;
if (mspOut)
*mspOut = msp;
/* If we only want to know if this coord is affected by a polyA signal, we can return now.
*
* If we need to return whether there are multiple sites at this coord, we can return
* after we've found the second on (i.e. after multiple is set to TRUE). */
if (trueOrNotRequested(multiple))
break;
}
}
return result;
}
/* Determine whether the given coord in the given frame/strand is affected by
* a polyA site */
static gboolean coordAffectedByPolyASite(const int dnaIdx,
const BlxStrand strand,
BlxContext *bc,
const MSP **mspOut, /* the variation we found */
gboolean *multiple)
{
gboolean result = FALSE;
/* Loop through all variations */
int i = 0;
const MSP *msp = mspArrayIdx(bc->featureLists[BLXMSP_POLYA_SITE], i);
for ( ; msp; msp = mspArrayIdx(bc->featureLists[BLXMSP_POLYA_SITE], ++i))
{
if (mspGetRefStrand(msp) == strand && valueWithinRange(dnaIdx, &msp->qRange))
{
/* If result has already been set, then there are multiple sites on this coord */
if (result && multiple)
*multiple = TRUE;
result = TRUE;
if (mspOut)
*mspOut = msp;
/* If we only want to know if this coord is affected by a polyA signal, we can return now.
*
* If we need to return whether there are multiple sites at this coord, we can return
* after we've found the second on (i.e. after multiple is set to TRUE). */
if (trueOrNotRequested(multiple))
break;
}
}
return result;
}
/* Draw a rectangle with an outline and a fill color */
static void drawRectangle(GdkDrawable *drawable,
GdkGC *gc,
GdkColor *fillColor,
GdkColor *outlineColor,
const int x,
const int y,
const int width,
const int height,
const gboolean drawLeft,
const gboolean drawRight,
const gboolean drawTop,
const gboolean drawBottom)
{
const int lineWidth = 1;
if (fillColor)
{
gdk_gc_set_foreground(gc, fillColor);
gdk_draw_rectangle(drawable, gc, TRUE, x, y, width, height);
}
if (outlineColor)
{
gdk_gc_set_foreground(gc, outlineColor);
gdk_gc_set_line_attributes(gc, lineWidth, GDK_LINE_SOLID, GDK_CAP_BUTT, GDK_JOIN_MITER);
int w = width - lineWidth;
int h = height - lineWidth;
if (drawLeft)
gdk_draw_line(drawable, gc, x, y, x, y + h);
if (drawRight)
gdk_draw_line(drawable, gc, x + w, y, x + w, y + h);
if (drawTop)
gdk_draw_line(drawable, gc, x, y, x + w, y);
if (drawBottom)
gdk_draw_line(drawable, gc, x, y + h, x + w, y + h);
//gdk_draw_rectangle(drawable, gc, FALSE, x, y, width - lineWidth, height - lineWidth);
}
}
/* Returns true if the given coord (which must be in nucleotide coords) is within the
* range of any MSP that is selected. Only considers MSPs that are on the given ref seq
* frame and strand (if given; otherwise considers all MSPs). */
static gboolean isCoordInSelectedMspRange(const BlxContext *bc,
const int dnaIdx,
const BlxStrand refSeqStrand,
const int refSeqFrame,
const BlxSeqType seqType)
{
gboolean inSelectedMspRange = FALSE;
/* Loop through all the selected sequences */
GList *seqItem = bc->selectedSeqs;
for ( ; seqItem && !inSelectedMspRange; seqItem = seqItem->next)
{
/* Loop through all the MSPs in this sequence */
const BlxSequence* const blxSeq = (const BlxSequence*)(seqItem->data);
GList *mspItem = blxSeq->mspList;
for ( ; mspItem && !inSelectedMspRange; mspItem = mspItem->next)
{
const MSP* const msp = (const MSP*)(mspItem->data);
const int mspFrame = mspGetRefFrame(msp, seqType);
if ((mspIsBlastMatch(msp) || mspIsBoxFeature(msp)) &&
(refSeqStrand == BLXSTRAND_NONE || mspGetRefStrand(msp) == refSeqStrand) &&
(refSeqFrame == UNSET_INT || mspFrame == refSeqFrame))
{
/* Passed-in coord is the nucleotide for base 1 in frame 1 of the current display index.
* We need to compare against base1 for the frame for the individual MSP, though, so
* we need to convert it: just add 1 base if we're in frame 2 or 2 bases if we're in frame3. */
int idx = dnaIdx;
if (bc->displayRev)
{
idx -= (mspFrame - 1);
}
else
{
idx += (mspFrame - 1);
}
inSelectedMspRange = valueWithinRange(idx, &msp->qRange);
}
}
}
return inSelectedMspRange;
}
/* Determine whether any highlighting needs to be done for snps in the ref seq */
static void getSnpHighlighting(DrawBaseData *data,
BlxContext *bc)
{
gboolean drawBackground = FALSE;
if (coordAffectedByVariation(data->dnaIdx, data->strand, bc, NULL,
&data->drawStart, &data->drawEnd, &data->drawJoiningLines, &drawBackground, NULL))
{
/* The coord is affected by a SNP. Outline it in the "selected" SNP color
* (which is darker than the normal color) */
data->outlineColor = getGdkColor(BLXCOLOR_SNP, bc->defaultColors, TRUE, bc->usePrintColors);
/* If the SNP is selected, also fill it with the SNP color (using the
* "unselected" SNP color, which is lighter than the outline). */
if (drawBackground)
{
data->fillColor = getGdkColor(BLXCOLOR_SNP, bc->defaultColors, data->shadeBackground, bc->usePrintColors);
}
}
}
/* Draw a given nucleotide or peptide. Determines the color depending on various
* parameters */
void drawHeaderChar(BlxContext *bc,
DetailViewProperties *properties,
GdkDrawable *drawable,
GdkGC *gc,
const int x,
const int y,
GHashTable *basesToHighlight,
DrawBaseData *data)
{
if (!data->selectionRange || (data->selectionRange && data->selectionRange->length() == 1))
{
/* Shade the background if the base is selected XOR if the base is within the range of a
* selected sequence. (If both conditions are true we don't shade, to give the effect of an
* inverted selection color.) */
gboolean inSelectedMspRange = isCoordInSelectedMspRange(bc, data->dnaIdx, data->strand, data->frame, data->seqType);
data->shadeBackground = (data->displayIdxSelected != inSelectedMspRange);
}
else
{
/* We have a range of selected coords. Just shade if this coord is in that range. */
data->shadeBackground = valueWithinRange(data->dnaIdx, data->selectionRange);
}
/* Reset background color and outlines to defaults */
data->outlineColor = NULL;
data->fillColor = NULL;
data->drawStart = FALSE;
data->drawEnd = FALSE;
data->drawJoiningLines = FALSE;
/* Check if this coord already has a special color stored for it */
gpointer hashValue = g_hash_table_lookup(basesToHighlight, GINT_TO_POINTER(data->dnaIdx));
if (hashValue)
{
BlxColorId colorId = (BlxColorId)GPOINTER_TO_INT(hashValue);
GdkColor *color = getGdkColor(colorId, bc->defaultColors, data->shadeBackground, bc->usePrintColors);
if (colorId == BLXCOLOR_POLYA_SITE_ANN)
{
/* For polyA sites we don't shade the background; instead we want to draw a bar after the
* coord (or before if the display is reversed), so set the outline.
* (NB Bit of a hack to use the colorId to identify polyA sites here but it works fine.) */
data->outlineColor = color;
if (bc->displayRev)
data->drawStart = TRUE;
else
data->drawEnd = TRUE;
}
else
{
/* Normal highlighting: just fill in the background with the specified color */
data->fillColor = color;
}
}
/* Check if this base is in the currently-selected codon and needs highlighting */
if (data->seqType == BLXSEQ_DNA && data->showCodons && (data->dnaIdxSelected || data->displayIdxSelected || data->shadeBackground))
{
if (data->dnaIdxSelected || data->displayIdxSelected)
{
/* The coord is a nucleotide in the currently-selected codon. The color depends
* on whether the actual nucleotide itself is selected, or just the codon that it
* belongs to. */
data->fillColor = getGdkColor(BLXCOLOR_CODON, bc->defaultColors, data->dnaIdxSelected, bc->usePrintColors);
}
else if (!data->fillColor)
{
/* The coord is not selected but this coord is within the range of a selected MSP, so
* shade the background. */
data->fillColor = getGdkColor(data->defaultBgColor, bc->defaultColors, data->shadeBackground, bc->usePrintColors);
}
}
/* Check if this base is a SNP (or other variation) and needs highlighting */
if (data->highlightSnps)
{
getSnpHighlighting(data, bc);
}
/* If the base is not already assigned some special highlighting, then
* check whether it should be highlighted as a stop or MET. Otherwise,
* give it the default background colour. */
if (!data->fillColor)
{
if (data->seqType == BLXSEQ_PEPTIDE && data->baseChar == SEQUENCE_CHAR_MET)
{
/* The coord is a MET codon */
data->fillColor = getGdkColor(BLXCOLOR_MET, bc->defaultColors, data->shadeBackground, bc->usePrintColors);
}
else if (data->seqType == BLXSEQ_PEPTIDE && data->baseChar == SEQUENCE_CHAR_STOP)
{
/* The coord is a STOP codon */
data->fillColor = getGdkColor(BLXCOLOR_STOP, bc->defaultColors, data->shadeBackground, bc->usePrintColors);
}
else if (data->showBackground)
{
/* Use the default background color for the reference sequence */
data->fillColor = getGdkColor(data->defaultBgColor, bc->defaultColors, data->shadeBackground, bc->usePrintColors);
}
}
/* Ok, now we know all the colours and outlines, draw the background for the base */
if (data->topToBottom)
{
/* We're drawing nucleotides from top-to-bottom instead of left-to-right, so the start border is
* the top border and the bottom border is the end border. */
drawRectangle(drawable, gc, data->fillColor, data->outlineColor, x, y, ceil(properties->charWidth()), roundNearest(properties->charHeight()),
data->drawJoiningLines, data->drawJoiningLines, data->drawStart, data->drawEnd);
}
else
{
drawRectangle(drawable, gc, data->fillColor, data->outlineColor, x, y, ceil(properties->charWidth()), roundNearest(properties->charHeight()),
data->drawStart, data->drawEnd, data->drawJoiningLines, data->drawJoiningLines);
}
}
/* This works out the row number (1-based) to draw a variation with the given range
* in. Variations are drawn in row 1 if possible, but if the given range overlaps
* another variation in row 1, then we will try row 2, then row 3 etc.
* If numRows is UNSET_INT, then the straightforward row calculation is returned.
* If numRows is given, then the result is inverted (i.e. if there are three rows, rows
* 1 is translated to 3, 2->2 and 3->1. This is so that we can draw the first row at the
* bottom). */
static int getVariationRowNumber(const IntRange* const rangeIn, const int numRows, GSList **rows)
{
/* Loop through each row and add it to the first row where it will not overlap any other */
GSList *row = *rows;
int rowNum = 1;
for ( ; row; row = row->next, ++rowNum)
{
/* See if it overlaps an existing range */
GSList *ranges = (GSList*)(row->data);
GSList *rangeItem = ranges;
gboolean overlaps = FALSE;
for ( ; rangeItem && !overlaps; rangeItem = rangeItem->next)
{
IntRange *range = (IntRange*)(rangeItem->data);
overlaps = rangesOverlap(range, rangeIn);
}
if (!overlaps)
{
/* Doesn't overlap anything in this row; add it to the row and exit */
IntRange *range = new IntRange;
range->set(rangeIn);
ranges = g_slist_append(ranges, range);
row->data = ranges;
if (numRows != UNSET_INT)
rowNum = numRows - rowNum + 1; /* invert row order */
return rowNum;
}
}
/* If we get here, we didn't find a row that we don't overlap, so add a new row */
GSList *ranges = NULL;
IntRange *range = new IntRange;
range->set(rangeIn);
ranges = g_slist_append(ranges, range);
*rows = g_slist_append(*rows, ranges);
if (numRows != UNSET_INT)
rowNum = numRows - rowNum + 1; /* invert row order */
return rowNum;
}
/* This deletes the memory allocated to the 'rows' array created
* when drawing the snp track */
static void freeRowsList(GSList *rows)
{
GSList *rowItem = rows;
for ( ; rowItem; rowItem = rowItem->next)
{
GSList *ranges = (GSList*)(rowItem->data);
GSList *rangeItem = ranges;
for ( ; rangeItem; rangeItem = rangeItem->next)
{
IntRange *range = (IntRange*)(rangeItem->data);
delete range;
}
g_slist_free(ranges);
}
g_slist_free(rows);
}
/* Utility to calculate the number of rows we need in the SNP track to avoid
* any overlapping variations. */
static int getNumSnpTrackRows(const BlxContext *bc,
DetailViewProperties *properties,
const BlxStrand strand,
const int frame)
{
DEBUG_ENTER("getNumSnpTrackRows()");
int numRows = 0; /* Always show one row, even if it is empty */
int i = 0;
const MSP* msp = mspArrayIdx(bc->featureLists[BLXMSP_VARIATION], i);
GSList *rows = NULL;
for ( ; msp; msp = mspArrayIdx(bc->featureLists[BLXMSP_VARIATION], ++i))
{
if (mspGetRefStrand(msp) == strand && mspGetMatchSeq(msp))
{
/* Get the range of display coords where this SNP will appear */
IntRange mspDisplayRange, mspExpandedRange;
getVariationDisplayRange(msp, TRUE, bc->seqType, bc->numFrames, bc->displayRev, frame, &bc->refSeqRange, &mspDisplayRange, &mspExpandedRange);
/* See if the variation is in the current display range */
if (rangesOverlap(&mspExpandedRange, &properties->displayRange))
{
/* Get the row number (1-based) to draw this variation in. */
int row = getVariationRowNumber(&mspExpandedRange, UNSET_INT, &rows);
/* Keep track of how many rows we've seen so we can size the widget accordingly */
if (row > numRows)
numRows = row;
}
}
}
freeRowsList(rows);
DEBUG_EXIT("getNumSnpTrackRows returning %d", numRows);
return numRows;
}
/* Recalculate the size of the variations track. This needs to be done whenever
* we turn the track on/off, and also whenever we we scroll, because more/less
* variations can appear and if they overlap then we add extra rows in which to
* draw them. */
static void recalculateSnpTrackBorders(GtkWidget *snpTrack, gpointer data)
{
DEBUG_ENTER("recalculateSnpTrackBorders()");
//GtkWidget *detailView = GTK_WIDGET(data);
//snpTrackSetHeight(detailView, snpTrack);
DEBUG_EXIT("recalculateSnpTrackBorders returning ");
}
/* Function that does the drawing for the variations track */
static void drawVariationsTrack(GtkWidget *snpTrack, GtkWidget *detailView)
{
DEBUG_ENTER("drawVariationsTrack()");
/* Create the drawable for the widget (whether we're actually going to do any drawing or not) */
DetailViewProperties *properties = detailViewGetProperties(detailView);
GdkDrawable *drawable = createBlankSizedPixmap(snpTrack, snpTrack->window, snpTrack->allocation.width, ceil(properties->charHeight()) * 10);
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
BlxContext *bc = blxWindowGetContext(blxWindow);
if (!bc->flags[BLXFLAG_SHOW_VARIATION_TRACK] || !bc->flags[BLXFLAG_HIGHLIGHT_VARIATIONS])
{
DEBUG_EXIT("drawVariationsTrack returning");
return;
}
const int activeFrame = detailViewGetActiveFrame(detailView);
BlxStrand strand = snpTrackGetStrand(snpTrack, detailView);
GdkGC *gc = gdk_gc_new(drawable);
/* Find the left margin. It will be at the same x coord as the left edge of
* the sequence column header. */
int leftMargin = UNSET_INT;
BlxColumnInfo *seqColInfo = getColumnInfo(detailViewGetColumnList(detailView), BLXCOL_SEQUENCE);
gtk_widget_translate_coordinates(seqColInfo->headerWidget, snpTrack, 0, 0, &leftMargin, NULL);
/* Maintain lists for each row where the variations are drawn; remember their display
* ranges and don't allow any to overlap. */
const int numRows = getNumSnpTrackRows(bc, properties, strand, activeFrame);
/* Loop through all variations and see if any are in the current display range */
const int y = 0;
const int rowHeight = ceil(properties->charHeight());
int i = 0;
const MSP *msp = mspArrayIdx(bc->featureLists[BLXMSP_VARIATION], i);
GSList *rows = NULL;
for ( ; msp; msp = mspArrayIdx(bc->featureLists[BLXMSP_VARIATION], ++i))
{
if (mspGetRefStrand(msp) == strand && mspGetMatchSeq(msp))
{
/* Get the range of display coords where this SNP will appear */
IntRange mspDisplayRange, mspExpandedRange;
getVariationDisplayRange(msp, TRUE, bc->seqType, bc->numFrames, bc->displayRev, activeFrame, &bc->refSeqRange, &mspDisplayRange, &mspExpandedRange);
/* See if the variation is in the current display range */
if (rangesOverlap(&mspExpandedRange, &properties->displayRange))
{
/* Get the row number to draw this variation in. */
const int rowNum = getVariationRowNumber(&mspExpandedRange, numRows, &rows);
const int rowIdx = rowNum - 1;
int x = leftMargin + (int)((gdouble)(mspExpandedRange.min() - properties->displayRange.min()) * properties->charWidth());
const int width = ceil((gdouble)strlen(mspGetMatchSeq(msp)) * properties->charWidth());
const gboolean isSelected = blxWindowIsSeqSelected(blxWindow, msp->sSequence);
/* Draw the outline in the default SNP color. If the SNP is selected, also
* fill in the rectangle in the SNP color (use the selected color for the
* outline and the unselected color for the fill, so that the outline is darker). */
GdkColor *outlineColor = getGdkColor(BLXCOLOR_SNP, bc->defaultColors, TRUE, bc->usePrintColors);
GdkColor *fillColor = isSelected ? getGdkColor(BLXCOLOR_SNP, bc->defaultColors, FALSE, bc->usePrintColors) : NULL;
/* Draw the background rectangle for the char */
drawRectangle(drawable, gc, fillColor, outlineColor, x, y + (rowHeight * rowIdx), width, rowHeight, TRUE, TRUE, TRUE, TRUE);
/* Draw the text */
PangoLayout *layout = gtk_widget_create_pango_layout(detailView, mspGetMatchSeq(msp));
pango_layout_set_font_description(layout, properties->fontDesc);
if (layout)
{
gtk_paint_layout(snpTrack->style, drawable, GTK_STATE_NORMAL, TRUE, NULL, detailView, NULL, x, y + (rowHeight * rowIdx), layout);
g_object_unref(layout);
}
}
}
}
freeRowsList(rows);
drawColumnSeparatorLine(snpTrack, drawable, gc, bc);
g_object_unref(gc);
DEBUG_EXIT("drawVariationsTrack returning ");
}
/* This function centres the detail view display on the currently-selected base index. Does
* nothing if there isn't a selected base. */
static void detailViewCentreOnSelection(GtkWidget *detailView)
{
DEBUG_ENTER("detailViewCentreOnSelection()");
const gboolean selectedBaseSet = detailViewGetSelectedIdxSet(detailView);
const int selectedBaseIdx = detailViewGetSelectedDisplayIdx(detailView);
if (selectedBaseSet)
{
/* The coord is in terms of the display coords, i.e. whatever the displayed seq type is. */
const BlxSeqType seqType = detailViewGetSeqType(detailView);
const IntRange* const displayRange = detailViewGetDisplayRange(detailView);
int newStart = selectedBaseIdx - (displayRange->length() / 2);
setDetailViewStartIdx(detailView, newStart, seqType);
}
DEBUG_EXIT("detailViewCentreOnSelection returning ");
}
/* In the detail view, get the base index at the given coords, if those coords lie within the
* sequence column; returns UNSET_INT otherwise. */
static int getBaseIndexAtDetailViewCoords(GtkWidget *detailView, const int x, const int y)
{
int baseIdx = UNSET_INT;
DetailViewProperties *properties = detailViewGetProperties(detailView);
GList *columnList = detailViewGetColumnList(detailView);
/* Get the x coords at the start/end of the sequence column */
IntRange xRange;
getColumnXCoords(columnList, BLXCOL_SEQUENCE, &xRange);
/* See if our x coord lies inside the sequence column */
if (x >= xRange.min() && x <= xRange.max())
{
/* Get the 0-based char index at x */
gdouble charWidth = properties->charWidth();
int charIdx = (int)(((gdouble)x - xRange.min()) / charWidth);
/* Add the start of the scroll range to convert this to the display index */
GtkAdjustment *adjustment = properties->adjustment;
baseIdx = charIdx + adjustment->value;
}
return baseIdx;
}
/***********************************************************
* Detail view scrollbar events *
***********************************************************/
/* Callback called when the detail-view scrollbar's range has changed */
static void onScrollRangeChangedDetailView(GtkObject *object, gpointer data)
{
DEBUG_ENTER("onScrollRangeChangedDetailView");
GtkAdjustment *adjustment = GTK_ADJUSTMENT(object);
GtkWidget *detailView = GTK_WIDGET(data);
IntRange *displayRange = detailViewGetDisplayRange(detailView);
/* First time round, set the adjusment range to be centred on the centre of
* the detail-view range*/
if (adjustment->value == UNSET_INT)
{
adjustment->value = displayRange->centre() - (adjustment->page_size / 2);
if (adjustment->value < adjustment->lower)
adjustment->value = adjustment->lower;
else if (adjustment->value > adjustment->upper - adjustment->page_size + 1)
adjustment->value = adjustment->upper - adjustment->page_size + 1;
}
int newStart = adjustment->value;
int newEnd = newStart + adjustment->page_size - 1;
/* Only update if something has changed */
if (displayRange->min() != newStart || displayRange->max() != newEnd)
{
IntRange oldRange = {displayRange->min(), displayRange->max()};
displayRange->set(newStart, newEnd);
/* Refilter the data for all trees in the detail view because rows may have scrolled in/out of view */
refilterDetailView(detailView, &oldRange);
/* Refresh the detail view header (which may contain the DNA sequence), and
* the headers for all the trees (which contains the reference sequence) */
refreshDetailViewHeaders(detailView);
callFuncOnAllDetailViewTrees(detailView, refreshTreeHeaders, NULL);
detailViewRedrawAll(detailView);
/* Update the big picture because the highlight box has moved (and changed size) */
GtkWidget *bigPicture = detailViewGetBigPicture(detailView);
refreshBigPictureDisplayRange(bigPicture, FALSE);
calculateBigPictureCellSize(bigPicture, bigPictureGetProperties(bigPicture));
}
DEBUG_EXIT("onScrollRangeChangedDetailView returning");
}
/* Callback called when the detail-view scrollbar's position has changed */
static void onScrollPosChangedDetailView(GtkObject *object, gpointer data)
{
DEBUG_ENTER("onScrollPosChangedDetailView");
GtkAdjustment *adjustment = GTK_ADJUSTMENT(object);
GtkWidget *detailView = GTK_WIDGET(data);
/* Set the display range so that it starts at the new scroll pos */
int newStart = adjustment->value;
int newEnd = adjustment->value + adjustment->page_size - 1;
/* Only update if something has changed */
DetailViewProperties *properties = detailViewGetProperties(detailView);
IntRange *displayRange = &properties->displayRange;
if (displayRange->min() != newStart || displayRange->max() != newEnd)
{
IntRange oldRange(displayRange->min(), displayRange->max());
displayRange->set(newStart, newEnd);
/* Refilter the data for all trees in the detail view because rows may have scrolled in/out of view */
refilterDetailView(detailView, &oldRange);
/* Refresh the detail view header (which may contain the DNA sequence), and
* the headers for all the trees (which contains the reference sequence) */
refreshDetailViewHeaders(detailView);
callFuncOnAllDetailViewTrees(detailView, refreshTreeHeaders, NULL);
detailViewRedrawAll(detailView);
/* Update the big picture because the highlight box has moved */
GtkWidget *bigPicture = blxWindowGetBigPicture(properties->blxWindow());
refreshBigPictureDisplayRange(bigPicture, FALSE);
}
DEBUG_EXIT("onScrollPosChangedDetailView returning");
}
/***********************************************************
* Detail View toolbar events *
***********************************************************/
/* Set the font for the detail view cell renderer. Should be called after
* the font size is changed by zooming in/out of the detail view. */
static void updateCellRendererFont(GtkWidget *detailView, PangoFontDescription *fontDesc)
{
/* Calculate the row height from the font size */
gdouble charWidth, charHeight;
getFontCharSize(detailView, fontDesc, &charWidth, &charHeight);
/* Cache these results, because we use them often for calculations */
DetailViewProperties *properties = detailViewGetProperties(detailView);
properties->setFontSize(charWidth, charHeight);
/* Set the row height. Subtract the padding between the cell's actual area and
* its background area. We will render at the background area's height, so that
* we draw over the "gaps" between the cells, giving the impression of no gaps. */
gint rowHeight = roundNearest(charHeight) - (detailViewGetCellYPadding(detailView) * 2);
GtkCellRenderer *renderer = detailViewGetRenderer(detailView);
gtk_cell_renderer_set_fixed_size(renderer, 0, rowHeight);
}
/* Refilter the tree row that this msp is in */
static void refilterMspRow(MSP *msp, GtkWidget *detailView, BlxContext *bc)
{
//DEBUG_ENTER("refilterMspRow(msp=[%d,%d])", msp->fullRange.min, msp->fullRange.max);
/* Find the tree row that this MSP is in and force that row to update
* its visibility status. */
gchar *pathStr = mspGetTreePath(msp, bc->modelId);
if (pathStr)
{
GtkTreePath *path = gtk_tree_path_new_from_string(pathStr);
if (path)
{
GtkWidget *tree = detailViewGetTree(detailView, mspGetRefStrand(msp), mspGetRefFrame(msp, bc->seqType));
GtkTreeModel *model = treeGetBaseDataModel(GTK_TREE_VIEW(tree));
GtkTreeIter iter;
if (gtk_tree_model_get_iter(model, &iter, path))
{
gtk_tree_model_row_changed(model, path, &iter);
}
}
gtk_tree_path_free(path);
}
//DEBUG_EXIT("refilterMspRow returning ");
}
/* Utility to see if the start value of the first range is within the second
* range. If 'rev' is true, the 'start' of range1 is the max value rather than
* the min value of this range. */
static gboolean startValueWithinRange(const IntRange* const range1,
const IntRange* const range2,
const gboolean rev)
{
gboolean result = FALSE;
if (rev)
result = valueWithinRange(range1->max(), range2);
else
result = valueWithinRange(range1->min(), range2);
return result;
}
/* Quick search to find any MSP in the given array that whose start coord
* lies within the given range. Sets the found array index in 'idx' or returns
* FALSE if no MSP was found in this range. */
static gboolean getAnyMspInRange(GArray *mspArray,
const IntRange* const range,
const gboolean displayRev,
int *idx)
{
gboolean result = FALSE;
/* Do a binary search based on start coord until we find a start coord
* that lies within the given range. */
int iMax = mspArray->len - 1;
int iMin = 0;
while (1)
{
const int i = iMin + (iMax - iMin) / 2;
const MSP *msp = mspArrayIdx(mspArray, i);
if (!msp)
break;
if (startValueWithinRange(&msp->displayRange, range, displayRev))
{
result = TRUE;
*idx = i;
break;
}
if (iMax - iMin < 1)
break;
if ((msp->displayRange.min() < range->min()) != displayRev)
{
iMin = i + 1;
}
else
{
iMax = i - 1;
}
}
return result;
}
/* Refilter the tree rows for all MSPs in the given array whose start coords
* lie within the given range. 'startIdx' should be an index in the array that
* gives the position of an MSP that is known to lie within this range (i.e.
* it gives us a rough starting point so that we don't have to search through
* the entire array; it does not necessarily have to be the FIRST MSP that lies
* within range). */
static void refilterMspList(const int startIdx,
GArray *array,
const IntRange* const range,
GtkWidget *detailView,
BlxContext *bc)
{
DEBUG_ENTER("refilterMspList(startIdx=%d, range=[%d,%d])", startIdx, range->min(), range->max());
/* Loop forwards through the array from the start index, refiltering the rows
* for each MSP until we find an MSP that is out of range. */
int i = startIdx;
MSP *msp = mspArrayIdx(array, i);
for ( ; msp; msp = mspArrayIdx(array, ++i))
{
if (!startValueWithinRange(&msp->displayRange, range, bc->displayRev))
break;
refilterMspRow(msp, detailView, bc);
}
/* Also loop backwards from the start index, because startIdx may not have
* been the very first MSP in the array that was in range. */
i = startIdx - 1;
msp = mspArrayIdx(array, i);
for ( ; msp; msp = mspArrayIdx(array, --i))
{
if (!startValueWithinRange(&msp->displayRange, range, bc->displayRev))
break;
refilterMspRow(msp, detailView, bc);
}
DEBUG_EXIT("refilterMspList returning ");
}
/* Refilter rows in the detail-view trees for MSPs of the given type. Only filter
* the rows of MSPs that lie within the given display range. */
static void refilterMspArrayByRange(BlxMspType mspType,
BlxContext *bc,
const IntRange* const displayRange,
GtkWidget *detailView)
{
//DEBUG_ENTER("refilterMspArrayByRange(mspType=%d)", mspType);
if (!displayRange)
return;
/* We only want to update MSPs that are within the given display range.
*
* We want to avoid searching through the entire MSP array, because it may
* contain many thousands of MSPs. However, it is difficult to do a quick
* search to find an item from the list based on two values (i.e. the start and
* end of its range).
* What we do is extend the start of the display range by the maximum MSP
* length so that we can do a binary search just on the start coord of the MSP
* to check that it lies within the extended range. For any MSP that lies within
* the display range, it must be true that its start coord lies within this
* extended range.
* Because the extended range is based on the maximum MSP length, it is likely
* to include some MSPs that do not actually lie within the display range.
* However, there is no harm in filtering a few extra rows, and this should
* still be more efficient that searching through the entire array of MSPs. */
const int maxLen = getMaxMspLen();
/* If the display is reversed, the 'start' is the maximum coord end
* rather than the minimum coord */
IntRange extendedRange;
if (bc->displayRev)
{
extendedRange.set(displayRange->min(), displayRange->max() + maxLen);
}
else
{
extendedRange.set(displayRange->min() - maxLen, displayRange->max());
}
int startIdx = 0;
if (getAnyMspInRange(bc->featureLists[mspType], &extendedRange, bc->displayRev, &startIdx))
{
refilterMspList(startIdx, bc->featureLists[mspType], &extendedRange, detailView, bc);
}
//DEBUG_EXIT("refilterMspArrayByRange returning ");
}
/* Refilter the rows in the detail-view trees. Only updates the rows in the
* current display range and (if given) the old display range. */
void refilterDetailView(GtkWidget *detailView, const IntRange* const oldRange)
{
DEBUG_ENTER("refilterDetailView(oldRange=[%d,%d])", oldRange ? oldRange->min() : 0, oldRange ? oldRange->max() : 0);
BlxContext *bc = detailViewGetContext(detailView);
if (bc->flags[BLXFLAG_SHOW_POLYA_SITE] || bc->flags[BLXFLAG_SHOW_UNALIGNED])
{
/* If these options are enabled, then determining which rows need to be
* refiltered becomes much more complex because visible alignments lengths
* may differ from the actual alignment coords, and may differ depending
* on whether the sequence is selected or not. For now, play safe and
* refilter everything. */
callFuncOnAllDetailViewTrees(detailView, refilterTree, NULL);
}
else
{
/* We only want to update MSPs that are in the old detail-view range
* and the new detail-view range. (Because updating every row in all
* trees can be very slow if there are a lot of MSPs.) */
const IntRange* const newRange = detailViewGetDisplayRange(detailView);
/* Only consider MSPs that are shwon in the detail-view */
int mspType = 0;
for ( ; mspType < BLXMSP_NUM_TYPES; ++mspType)
{
if (typeShownInDetailView((BlxMspType)mspType))
{
refilterMspArrayByRange((BlxMspType)mspType, bc, oldRange, detailView);
refilterMspArrayByRange((BlxMspType)mspType, bc, newRange, detailView);
}
}
detailViewRedrawAll(detailView);
}
DEBUG_EXIT("refilterDetailView returning ");
}
/***********************************************************
* Properties *
***********************************************************/
static void assertDetailView(GtkWidget *detailView)
{
/* Check it's a valid detail-view tree type */
if (!detailView)
g_error("Detail-view widget is null\n");
if (!GTK_IS_WIDGET(detailView))
g_error("Detail-view is not a valid widget [%p]\n", detailView);
if (!GTK_IS_CONTAINER(detailView))
g_error("Detail-view is not a valid container [%p]\n", detailView);
if (!detailViewGetProperties(detailView))
g_error("Tree properties not set [widget=%p]\n", detailView);
}
GtkWidget* detailViewGetBlxWindow(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->blxWindow() : NULL;
}
GtkAdjustment* detailViewGetAdjustment(GtkWidget *detailView)
{
assertDetailView(detailView);
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->adjustment : NULL;
}
GtkCellRenderer* detailViewGetRenderer(GtkWidget *detailView)
{
assertDetailView(detailView);
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->renderer : NULL;
}
/* Get the list of columns */
GList* detailViewGetColumnList(GtkWidget *detailView)
{
BlxContext *bc = detailViewGetContext(detailView);
return bc ? bc->columnList : NULL;
}
/* For the given list of columns, extract their types into an
* array of 'GType's */
GType* columnListGetTypes(GList *columnList)
{
GType *result = NULL;
const int len = g_list_length(columnList);
if (len > 0)
{
result = (GType*)g_malloc(sizeof(GType) * len);
GList *item = columnList;
int i = 0;
for ( ; item; item = item->next, ++i)
{
BlxColumnInfo* columnInfo = (BlxColumnInfo*)(item->data);
result[i] = columnInfo->type;
}
}
return result;
}
gboolean detailViewGetDisplayRev(GtkWidget *detailView)
{
return blxWindowGetDisplayRev(detailViewGetBlxWindow(detailView));
}
PangoFontDescription *detailViewGetFontDesc(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->fontDesc : NULL;
}
int detailViewGetCellXPadding(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->cellXPadding : 0;
}
int detailViewGetCellYPadding(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->cellYPadding : 0;
}
/* Get the character width. */
gdouble detailViewGetCharWidth(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->charWidth() : 0.0;
}
gdouble detailViewGetCharHeight(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->charHeight() : 0.0;
}
/* Get the outermost container for the GtkTreeView for the given frame on the given strand */
GtkWidget* detailViewGetTreeContainer(GtkWidget *detailView, const BlxStrand activeStrand, const int frame)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
GtkWidget *result = NULL;
/* Get the list of trees for the relevant strand */
GList *list = (activeStrand == BLXSTRAND_FORWARD) ? properties->fwdStrandTrees : properties->revStrandTrees;
/* The list should be sorted in order of frame number, and we should not be requesting a frame number
* greater than the number of items in the list */
if (frame <= (int)g_list_length(list))
{
GList *listItem = list;
int count = 1;
for ( ; listItem && count < frame; ++count)
{
listItem = listItem->next;
}
if (count == frame &&
GTK_IS_WIDGET(listItem->data) &&
(widgetIsTree(GTK_WIDGET(listItem->data)) || widgetIsTreeContainer(GTK_WIDGET(listItem->data))))
{
result = GTK_WIDGET(listItem->data);
}
}
return result;
}
/* Extract the actual GtkTreeView for the given frame on the given strand */
GtkWidget* detailViewGetTree(GtkWidget *detailView, const BlxStrand activeStrand, const int frame)
{
GtkWidget *result = NULL;
GtkWidget *treeContainer = detailViewGetTreeContainer(detailView, activeStrand, frame);
if (treeContainer && widgetIsTree(treeContainer))
{
result = treeContainer;
}
else if (treeContainer)
{
result = treeContainerGetTree(GTK_CONTAINER(treeContainer));
}
if (!result)
{
g_warning("Tree not found for '%s' strand, frame '%d'. Returning NULL.\n", ((activeStrand == BLXSTRAND_FORWARD) ? "forward" : "reverse"), frame);
}
return result;
}
int detailViewGetNumFrames(GtkWidget *detailView)
{
BlxContext *bc = detailViewGetContext(detailView);
return bc->numFrames;
}
static GtkWidget* detailViewGetFeedbackBox(GtkWidget *detailView)
{
DetailViewProperties *detailViewProperties = detailViewGetProperties(detailView);
return detailViewProperties->feedbackBox;
}
IntRange* detailViewGetDisplayRange(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? &properties->displayRange : NULL;
}
IntRange* detailViewGetRefSeqRange(GtkWidget *detailView)
{
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
return blxWindowGetRefSeqRange(blxWindow);
}
BlxSeqType detailViewGetSeqType(GtkWidget *detailView)
{
BlxContext *bc = detailViewGetContext(detailView);
return bc->seqType;
}
/* Returns true if the selected index is set */
gboolean detailViewGetSelectedIdxSet(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties && properties->selectedIndex ? properties->selectedIndex->isSet : FALSE;
}
/* Return the last-selected coord in display coords */
int detailViewGetSelectedDisplayIdx(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties && properties->selectedIndex && properties->selectedIndex->isSet ? properties->selectedIndex->displayIdx : UNSET_INT;
}
/* Return the initial index which initiated a selection range */
static DetailViewIndex *detailViewGetInitSelectedIndexStruct(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? &properties->selectedRangeInit : NULL;
}
/* Return the "first" end of the selection range, which is the other end to the last-selected
* index (i.e. if the start of the range was selected last, return the end of the range, and vice versa) */
static DetailViewIndex *detailViewGetFirstSelectedIndexStruct(GtkWidget *detailView)
{
DetailViewIndex *result = NULL;
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties)
{
DetailViewIndex *lastIdx = properties->selectedIndex;
if (lastIdx == &properties->selectedRangeStart)
result = &properties->selectedRangeEnd;
else
result = &properties->selectedRangeStart;
}
return result;
}
/* Return the last-selected index struct */
static DetailViewIndex *detailViewGetLastSelectedIndexStruct(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->selectedIndex : NULL;
}
/* Return the last-selected coord in dna coords */
int detailViewGetSelectedDnaIdx(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties && properties->selectedIndex && properties->selectedIndex->isSet ? properties->selectedIndex->dnaIdx : UNSET_INT;
}
/* Return true if a range of coords is selected (rather than a single coord) */
gboolean detailViewGetSelectedIdxRangeSet(GtkWidget *detailView)
{
gboolean result = FALSE;
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties &&
properties->selectedRangeStart.isSet &&
properties->selectedRangeEnd.isSet &&
properties->selectedRangeStart.dnaIdx != properties->selectedRangeEnd.dnaIdx)
{
result = TRUE;
}
return result;
}
/* Return the selection range in display coords. Allocates a new IntRange* which should be
* free'd by the caller with delete */
IntRange *detailViewGetSelectedDisplayIdxRange(GtkWidget *detailView)
{
IntRange *result = NULL;
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties && properties->selectedRangeStart.isSet && properties->selectedRangeEnd.isSet)
{
result = new IntRange;
result->set(properties->selectedRangeStart.displayIdx, properties->selectedRangeEnd.displayIdx);
}
return result;
}
/* Return the selection range in dna coords. Allocates a new IntRange* which should be
* free'd by the caller with delete */
IntRange *detailViewGetSelectedDnaIdxRange(GtkWidget *detailView)
{
IntRange *result = NULL;
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties && properties->selectedRangeStart.isSet && properties->selectedRangeEnd.isSet)
{
result = new IntRange;
result->set(properties->selectedRangeStart.dnaIdx, properties->selectedRangeEnd.dnaIdx);
}
return result;
}
/* Get the active frame. Returns the last-selected frame, or 1 if no frame is selected. */
int detailViewGetActiveFrame(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
int result = 1;
if (detailViewGetSelectedIdxSet(detailView))
{
result = properties->selectedIndex->frame;
}
return result;
}
/* Get the strand of the tree that was last selected (which defaults to the active strand if none is selected). */
BlxStrand detailViewGetSelectedStrand(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->selectedStrand : BLXSTRAND_FORWARD;
}
/* Set the strand of the tree that was last selected. */
void detailViewSetSelectedStrand(GtkWidget *detailView, BlxStrand strand)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
properties->selectedStrand = strand;
}
int detailViewGetNumUnalignedBases(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->numUnalignedBases : FALSE;
}
static int detailViewGetSnpConnectorHeight(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->snpConnectorHeight : UNSET_INT;
}
BlxColumnId* detailViewGetSortColumns(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties ? properties->sortColumns : NULL;
}
/* Perform required updates after the selected base has changed. */
static void updateFollowingBaseSelection(GtkWidget *detailView,
const gboolean allowScroll,
const gboolean scrollMinimum)
{
if (allowScroll)
{
scrollToKeepSelectionInRange(detailView, scrollMinimum);
}
/* Update the feedback box */
updateFeedbackBox(detailView);
/* Update the headers so that the newly-selected index is highlighted */
detailViewRefreshAllHeaders(detailView);
}
/* Return true if the given display index is in the selection range */
gboolean detailViewIsDisplayIdxSelected(GtkWidget *detailView, const int displayIdx)
{
gboolean result = FALSE;
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties)
{
/* Check if the coord is in the selection range */
IntRange *range = detailViewGetSelectedDisplayIdxRange(detailView);
if (range)
{
result = valueWithinRange(displayIdx, range);
delete range;
}
else if (detailViewGetSelectedIdxSet(detailView))
{
/* Check if the coord matches the selected index */
result = (displayIdx == detailViewGetSelectedDisplayIdx(detailView));
}
}
return result;
}
/* Return true if the given dna index is in the selection range */
gboolean detailViewIsDnaIdxSelected(GtkWidget *detailView, const int dnaIdx)
{
gboolean result = FALSE;
DetailViewProperties *properties = detailViewGetProperties(detailView);
if (properties)
{
IntRange *range = detailViewGetSelectedDnaIdxRange(detailView);
if (range)
{
/* Check if the coord is in the selection range */
result = valueWithinRange(dnaIdx, range);
delete range;
}
else if (detailViewGetSelectedIdxSet(detailView))
{
/* Check if the coord matches the selected index */
result = (dnaIdx == detailViewGetSelectedDnaIdx(detailView));
}
}
return result;
}
/* Utility to set the info in a DetailViewIndex struct */
static void setDetailViewIndex(DetailViewIndex *index,
const gboolean isSet,
const int dnaIdx,
const int displayIdx,
const int frame,
const int baseNum)
{
DEBUG_ENTER("setDetailViewIndex(%d, %d, %d, %d, %d)", isSet, dnaIdx, displayIdx, frame, baseNum);
if (index)
{
index->isSet = isSet;
index->dnaIdx = dnaIdx;
index->displayIdx = displayIdx;
index->frame = frame;
index->baseNum = baseNum;
}
DEBUG_EXIT("setDetailViewIndex returning ");
}
/* Cancel the current base index selection */
void detailViewUnsetSelectedBaseIdx(GtkWidget *detailView)
{
DEBUG_ENTER("detailViewUnsetSelectedBaseIdx()");
DetailViewProperties *properties = detailViewGetProperties(detailView);
setDetailViewIndex(&properties->selectedRangeInit, FALSE, UNSET_INT, UNSET_INT, UNSET_INT, UNSET_INT);
setDetailViewIndex(&properties->selectedRangeStart, FALSE, UNSET_INT, UNSET_INT, UNSET_INT, UNSET_INT);
setDetailViewIndex(&properties->selectedRangeEnd, FALSE, UNSET_INT, UNSET_INT, UNSET_INT, UNSET_INT);
properties->selectedIndex = NULL;
updateFollowingBaseSelection(detailView, FALSE, FALSE);
DEBUG_EXIT("detailViewUnsetSelectedBaseIdx returning ");
}
/* This function does the work to update the selected index (called by the display/dna specific
* functions). It makes sure the selection range is updated correctly when extending the
* range.
* Performs any required refreshes. Scrolls the view to keep the selected base
* in view if allowScroll is true. (Such scrolling is by the minimum
* number of bases necessary if scrollMinimum is true.)
* if extend is true, then extend the current range rather than starting a new
* selection. */
static void detailViewSetSelectedIndex(GtkWidget *detailView,
const int dnaIdx_in,
const int displayIdx_in,
const int frame,
const int baseNum,
const gboolean allowScroll,
const gboolean scrollMinimum,
const gboolean extend)
{
DEBUG_ENTER("detailViewSetSelectedIndex(%d, %d, %d, %d, %d)", dnaIdx_in, displayIdx_in, frame, baseNum, extend);
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
DetailViewProperties *properties = detailViewGetProperties(detailView);
int dnaIdx = dnaIdx_in;
int displayIdx = displayIdx_in;
/* Trim coords to the blixem range */
if (blxWindow)
{
IntRange *displayRange = blxWindowGetFullRange(blxWindow);
IntRange* dnaRange = blxWindowGetRefSeqRange(blxWindow);
boundsLimitValue(&displayIdx, displayRange);
boundsLimitValue(&dnaIdx, dnaRange);
}
if (!extend || !properties->selectedIndex ||
!properties->selectedRangeStart.isSet || !properties->selectedRangeEnd.isSet)
{
DEBUG_OUT("Setting selected index to %d\n", dnaIdx);
/* Nothing set or not extending - set the start and end of the range to be the same */
setDetailViewIndex(&properties->selectedRangeInit, TRUE, dnaIdx, displayIdx, frame, baseNum);
setDetailViewIndex(&properties->selectedRangeStart, TRUE, dnaIdx, displayIdx, frame, baseNum);
setDetailViewIndex(&properties->selectedRangeEnd, TRUE, dnaIdx, displayIdx, frame, baseNum);
/* The last-selected index can point to either start or end as they are the same */
properties->selectedIndex = &properties->selectedRangeStart;
}
else
{
DEBUG_OUT("Extending existing range (%d, %d) to %d\n",
properties->selectedRangeStart.dnaIdx, properties->selectedRangeEnd.dnaIdx, dnaIdx);
/* Extend or trim the existing range by setting the start or end of the range to the new
* value depending on whether the click was before or after the initial selection
* index. If the user clicked on the inital index, then trim the end that was last modified. */
const int initIdx = properties->selectedRangeInit.dnaIdx;
const int lastIdx = properties->selectedIndex->dnaIdx;
if (dnaIdx < initIdx || (dnaIdx == initIdx && lastIdx < initIdx))
{
setDetailViewIndex(&properties->selectedRangeStart, TRUE, dnaIdx, displayIdx, frame, baseNum);
properties->selectedIndex = &properties->selectedRangeStart;
}
if (dnaIdx > initIdx || (dnaIdx == initIdx && lastIdx > initIdx))
{
setDetailViewIndex(&properties->selectedRangeEnd, TRUE, dnaIdx, displayIdx, frame, baseNum);
properties->selectedIndex = &properties->selectedRangeEnd;
}
if (properties->selectedRangeStart.dnaIdx > properties->selectedRangeEnd.dnaIdx)
{
DEBUG_OUT("Warning: selection range start is greater than end!!\n");
}
}
updateFollowingBaseSelection(detailView, allowScroll, scrollMinimum);
DEBUG_EXIT("detailViewSetSelectedIndex returning ");
}
/* Set the selected base index to a specific DNA index. */
void detailViewSetSelectedDnaBaseIdx(GtkWidget *detailView,
const int dnaIdx,
const int frame,
const gboolean allowScroll,
const gboolean scrollMinimum,
const gboolean extend)
{
DEBUG_ENTER("detailViewSetSelectedDnaBaseIdx()");
BlxContext *bc = detailViewGetContext(detailView);
/* For protein matches, calculate the display index and base number of this dna idx */
int baseNum = UNSET_INT;
const int displayIdx = convertDnaIdxToDisplayIdx(dnaIdx, bc->seqType, frame, bc->numFrames, bc->displayRev, &bc->refSeqRange, &baseNum);
detailViewSetSelectedIndex(detailView, dnaIdx, displayIdx, frame, baseNum, allowScroll, scrollMinimum, extend);
DEBUG_EXIT("detailViewSetSelectedDnaBaseIdx returning ");
}
/* Set which frame is the active (currently-selected) reading frame */
void detailViewSetActiveFrame(GtkWidget *detailView, const int frame)
{
DEBUG_ENTER("detailViewSetActiveFrame()");
if (detailViewGetSelectedIdxSet(detailView))
{
/* Keep the selected DNA coord(s) the same but update the frame */
const int initIdx = detailViewGetInitSelectedIndexStruct(detailView)->dnaIdx;
const int firstIdx = detailViewGetFirstSelectedIndexStruct(detailView)->dnaIdx;
const int lastIdx = detailViewGetLastSelectedIndexStruct(detailView)->dnaIdx;
detailViewSetSelectedDnaBaseIdx(detailView, initIdx, frame, FALSE, FALSE, FALSE);
detailViewSetSelectedDnaBaseIdx(detailView, firstIdx, frame, FALSE, FALSE, TRUE);
detailViewSetSelectedDnaBaseIdx(detailView, lastIdx, frame, FALSE, FALSE, TRUE);
}
DEBUG_EXIT("detailViewSetActiveFrame returning ");
}
/* Set the selected base index to the given display index and base/frame number. */
void detailViewSetSelectedDisplayIdx(GtkWidget *detailView,
const int displayIdx,
const int frame,
const int baseNum,
const gboolean allowScroll,
const gboolean scrollMinimum,
const gboolean extend)
{
DEBUG_ENTER("detailViewSetSelectedDisplayIdx(%d, %d, %d)", displayIdx, frame, baseNum);
BlxContext *bc = detailViewGetContext(detailView);
/* For protein matches, calculate the base index in terms of the DNA sequence */
const int dnaIdx = convertDisplayIdxToDnaIdx(displayIdx,
bc->seqType,
frame,
baseNum,
bc->numFrames,
bc->displayRev,
&bc->refSeqRange);
detailViewSetSelectedIndex(detailView, dnaIdx, displayIdx, frame, baseNum, allowScroll, scrollMinimum, extend);
DEBUG_EXIT("detailViewSetSelectedDisplayIdx returning ");
}
/* Unselect and then reslect the currently selected coordinate(s). This makes sure the display
* coordinate agrees with the dna coord in the selection structs. */
static void detailViewRefreshSelection(GtkWidget *detailView)
{
if (detailViewGetSelectedIdxSet(detailView))
{
DetailViewIndex *init = detailViewGetInitSelectedIndexStruct(detailView);
DetailViewIndex *first = detailViewGetFirstSelectedIndexStruct(detailView);
DetailViewIndex *last = detailViewGetLastSelectedIndexStruct(detailView);
const int initIdx = init->dnaIdx;
const int firstIdx = first->dnaIdx;
const int lastIdx = last->dnaIdx;
const int initFrame = init->frame;
const int firstFrame = first->frame;
const int lastFrame = last->frame;
/* Unset, then select the inital index (with extend=false) */
detailViewUnsetSelectedBaseIdx(detailView);
detailViewSetSelectedDnaBaseIdx(detailView, initIdx, initFrame, FALSE, TRUE, FALSE);
/* Now set the extents, passing extend=true to extend either side of the init index */
detailViewSetSelectedDnaBaseIdx(detailView, firstIdx, firstFrame, FALSE, TRUE, TRUE);
detailViewSetSelectedDnaBaseIdx(detailView, lastIdx, lastFrame, FALSE, TRUE, TRUE);
}
}
int detailViewGetClickedBaseIdx(GtkWidget *detailView)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
return properties->clickedBaseIdx;
}
/* Set the selected base index to the given display index and base/frame number.
* Performs any required refreshes. Scrolls the view to keep the selected base
* in view if allowScroll is true. (Such scrolling is by the minimum
* number of bases necessary if scrollMinimum is true.) */
void detailViewSetClickedBaseIdx(GtkWidget *detailView,
const int clickedBaseIdx,
const int frame,
const int baseNum,
const gboolean allowScroll,
const gboolean scrollMinimum)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
/* For protein matches, calculate the base index in terms of the DNA sequence and cache it */
BlxContext *bc = detailViewGetContext(detailView);
properties->clickedBaseIdx = convertDisplayIdxToDnaIdx(clickedBaseIdx, bc->seqType, frame, baseNum, bc->numFrames, bc->displayRev, &bc->refSeqRange);
}
static GtkWidget *detailViewGetBigPicture(GtkWidget *detailView)
{
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
return blxWindowGetBigPicture(blxWindow);
}
DetailViewProperties* detailViewGetProperties(GtkWidget *widget)
{
/* optimisation: cache result, because we know there is only ever one detail view */
static DetailViewProperties *properties = NULL;
if (!properties && widget)
properties = (DetailViewProperties*)(g_object_get_data(G_OBJECT(widget), "DetailViewProperties"));
return properties;
}
static BlxContext* detailViewGetContext(GtkWidget *detailView)
{
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
return blxWindowGetContext(blxWindow);
}
static void onDestroyDetailView(GtkWidget *widget)
{
DetailViewProperties *properties = detailViewGetProperties(widget);
if (properties)
{
delete properties;
g_object_set_data(G_OBJECT(widget), "DetailViewProperties", NULL);
}
}
static DetailViewProperties* detailViewCreateProperties(GtkWidget *detailView,
GtkWidget *blxWindow,
BlxContext *bc,
CoverageViewProperties *coverageViewP,
GtkCellRenderer *renderer,
GList *fwdStrandTrees,
GList *revStrandTrees,
GtkWidget *feedbackBox,
GtkWidget *statusBar,
GList *columnList,
GtkAdjustment *adjustment,
const int startCoord,
const BlxColumnId sortColumn)
{
DetailViewProperties *properties = NULL;
if (detailView)
{
properties = new DetailViewProperties(detailView,
blxWindow,
bc,
coverageViewP,
renderer,
fwdStrandTrees,
revStrandTrees,
feedbackBox,
statusBar,
columnList,
adjustment,
startCoord,
sortColumn);
g_object_set_data(G_OBJECT(detailView), "DetailViewProperties", properties);
g_signal_connect(G_OBJECT(detailView), "destroy", G_CALLBACK(onDestroyDetailView), NULL);
}
return properties;
}
/* Get/set functions for the sequence column header's row number. (There is only
* one property to set, so it's not worth having a struct for the properties.) */
void seqColHeaderSetRow(GtkWidget *header, const int frame)
{
g_object_set_data(G_OBJECT(header), "seqColHeaderFrameNumber", GINT_TO_POINTER(frame));
}
int seqColHeaderGetRow(GtkWidget *header)
{
return header ? GPOINTER_TO_INT(g_object_get_data(G_OBJECT(header), "seqColHeaderFrameNumber")) : UNSET_INT;
}
/* Gets the base number within the given reading frame that this header row corresponds to*/
int seqColHeaderGetBase(GtkWidget *header, const int frame, const int numFrames)
{
const int row = seqColHeaderGetRow(header);
int baseNum = UNSET_INT;
if (row != UNSET_INT)
{
baseNum = row - (frame - 1);
/* Cyclic decrement */
if (baseNum < 1)
{
baseNum += numFrames;
}
}
return baseNum;
}
/* Get the coord at the given position in the sequence header */
static int seqColHeaderGetCoordAtPos(GtkWidget *header, GtkWidget *detailView, const int x, const int y)
{
int baseIdx = UNSET_INT;
GtkAdjustment *adjustment = detailViewGetAdjustment(detailView);
if (x >= 0 && x <= header->allocation.width)
{
/* Get the 0-based char index at x */
gdouble charWidth = detailViewGetCharWidth(detailView);
int charIdx = (int)((gdouble)x / charWidth);
/* Add the start of the scroll range to convert this to the display index */
baseIdx = charIdx + adjustment->value;
}
else if (x < 0)
{
baseIdx = adjustment->value;
}
else if (x > header->allocation.width)
{
baseIdx = adjustment->value = adjustment->page_size;
}
return baseIdx;
}
/***********************************************************
* Paned window properties
*
* This is requried for the SNP track paned windows, because
* we need to cache the splitter position when the user toggles
* it off so that we can reinstate it when the toggle back on.
***********************************************************/
static void panedWindowCreateProperties(GtkWidget *widget, const int splitterPos)
{
if (widget)
{
PanedWindowProperties *properties = new PanedWindowProperties;
properties->widget = widget;
properties->splitterPos = splitterPos;
g_object_set_data(G_OBJECT(widget), "PanedWindowProperties", properties);
g_signal_connect(G_OBJECT(widget), "destroy", G_CALLBACK(onDestroyPanedWindow), NULL);
}
}
static PanedWindowProperties* panedWindowGetProperties(GtkWidget *widget)
{
PanedWindowProperties *properties = NULL;
if (!properties && widget)
properties = (PanedWindowProperties*)(g_object_get_data(G_OBJECT(widget), "PanedWindowProperties"));
return properties;
}
static void onDestroyPanedWindow(GtkWidget *widget)
{
PanedWindowProperties *properties = panedWindowGetProperties(widget);
if (properties)
{
delete properties;
properties = NULL;
g_object_set_data(G_OBJECT(widget), "DetailViewProperties", NULL);
}
}
/* Get/set functions for the SNP track header strand. (There is only one property
* to set, so it's not worth having a struct for the properties.) */
static void snpTrackSetStrand(GtkWidget *snpTrack, const BlxStrand strand)
{
g_object_set_data(G_OBJECT(snpTrack), "snpTrackStrand", GINT_TO_POINTER(strand));
}
static BlxStrand snpTrackGetStrand(GtkWidget *snpTrack, GtkWidget *detailView)
{
BlxStrand strand = BLXSTRAND_NONE;
if (snpTrack)
{
strand = (BlxStrand)GPOINTER_TO_INT(g_object_get_data(G_OBJECT(snpTrack), "snpTrackStrand"));
}
if (strand == BLXSTRAND_NONE)
{
/* use the active strand instead */
strand = blxWindowGetActiveStrand(detailViewGetBlxWindow(detailView));
}
return strand;
}
static void snpTrackSetHeight(GtkWidget *detailView, GtkWidget *snpTrack)
{
DEBUG_ENTER("snpTrackSetHeight()");
BlxContext *bc = detailViewGetContext(detailView);
DetailViewProperties *properties = detailViewGetProperties(detailView);
GtkWidget *snpScrollWin = detailViewContainerGetParentWidget(GTK_CONTAINER(detailView), snpTrack, SNP_TRACK_SCROLL_WIN_NAME);
const int activeFrame = detailViewGetActiveFrame(detailView);
BlxStrand strand = snpTrackGetStrand(snpTrack, detailView);
const int numRows = getNumSnpTrackRows(bc, properties, strand, activeFrame);
const gdouble charHeight = detailViewGetCharHeight(detailView);
/* Set the height to the total character height plus the connector line height */
const int height = roundNearest(charHeight * (numRows + (gdouble)detailViewGetSnpConnectorHeight(detailView)));
gtk_layout_set_size(GTK_LAYOUT(snpTrack), snpTrack->allocation.width, height);
gtk_widget_set_size_request(snpTrack, -1, height);
gtk_widget_set_size_request(snpScrollWin, -1, height);
DEBUG_OUT("Strand %d, frame %d: Set SNP track height to %d\n", (int)strand, activeFrame, height);
/* Get the parent scrolled window and set the adjustment step increment to be one
* char height */
if (snpScrollWin && GTK_IS_SCROLLED_WINDOW(snpScrollWin))
{
GtkAdjustment *snpAdjustment = gtk_scrolled_window_get_vadjustment(GTK_SCROLLED_WINDOW(snpScrollWin));
if (snpAdjustment)
{
snpAdjustment->step_increment = (int)charHeight + detailViewGetSnpConnectorHeight(detailView);
}
}
else
{
g_warning("Error setting scroll increment for variations track\n");
DEBUG_OUT("Error setting scroll increment for variations track\n");
}
/* Now set the paned window splitter bar position. */
const gboolean showSnpTrack = bc->flags[BLXFLAG_SHOW_VARIATION_TRACK] && bc->flags[BLXFLAG_HIGHLIGHT_VARIATIONS];
GtkWidget *panedWidget = detailViewContainerGetParentWidget(GTK_CONTAINER(detailView), snpTrack, SNP_TRACK_CONTAINER_NAME);
GtkPaned *panedWin = GTK_IS_PANED(panedWidget) ? GTK_PANED(panedWidget) : NULL;
PanedWindowProperties *snpProperties = panedWindowGetProperties(GTK_WIDGET(panedWin));
if (panedWin && showSnpTrack)
{
/* Set it to the saved position, if there is one set */
if (snpProperties->splitterPos >= 0)
{
/* Use the cached position then reset the cache (because the splitter bar will keep
* track of it from here). */
DEBUG_OUT("Setting splitter position to %d\n", snpProperties->splitterPos);
/* gb10: actually I'm going to reset it to 'unset' because that's probably a bit more
* sensible when re-showing the snp track (which is the only time this cache gets
* used). Could do with tidying up this logic a bit. It might be nice to offer the
* option to use the cached position too. */
//gtk_paned_set_position(panedWin, snpProperties->splitterPos);
gtk_paned_set_position(panedWin, -1);
snpProperties->splitterPos = -1;
}
}
else if (panedWin)
{
/* Save the current value then set it to 0 to hide the SNP track */
int splitterPos = gtk_paned_get_position(panedWin);
if (splitterPos > 0)
{
DEBUG_OUT("Setting splitter position to 0 and caching old position %d\n", splitterPos);
snpProperties->splitterPos = splitterPos;
gtk_paned_set_position(panedWin, 0);
}
}
else
{
g_warning("Error setting splitter bar position for variations track\n");
DEBUG_OUT("Error setting splitter bar position for variations track\n");
}
DEBUG_EXIT("snpTrackSetHeight returning ");
}
/***********************************************************
* Events *
***********************************************************/
/* Expose function for generic text headers. Draws vertical separator lines and then lets the
* default handler continue. */
gboolean onExposeGenericHeader(GtkWidget *headerWidget, GdkEventExpose *event, gpointer data)
{
GdkGC *gc = gdk_gc_new(headerWidget->window);
GtkWidget *detailView = GTK_WIDGET(data);
const BlxContext *bc = detailViewGetContext(detailView);
drawColumnSeparatorLine(headerWidget, headerWidget->window, gc, bc);
g_object_unref(gc);
return FALSE;
}
/* expose function to push a cached bitmap to screen */
gboolean onExposeDnaTrack(GtkWidget *headerWidget, GdkEventExpose *event, gpointer data)
{
GdkWindow *window = GTK_IS_LAYOUT(headerWidget) ? GTK_LAYOUT(headerWidget)->bin_window : headerWidget->window;
if (window)
{
/* See if there's a cached drawable and, if not, create it */
GdkDrawable *bitmap = widgetGetDrawable(headerWidget);
if (!bitmap)
{
/* There isn't a bitmap yet. Create it now. */
GtkWidget *detailView = GTK_WIDGET(data);
const BlxStrand activeStrand = detailViewGetDisplayRev(detailView) ? BLXSTRAND_REVERSE : BLXSTRAND_FORWARD;
drawDnaTrack(headerWidget, detailView, activeStrand, seqColHeaderGetRow(headerWidget));
bitmap = widgetGetDrawable(headerWidget);
}
if (bitmap)
{
/* Push the bitmap onto the window */
GdkGC *gc = gdk_gc_new(window);
gdk_draw_drawable(window, gc, bitmap, 0, 0, 0, 0, -1, -1);
g_object_unref(gc);
}
}
return FALSE;
}
/* Expose handler for the variations-track header */
static gboolean onExposeVariationsTrack(GtkWidget *snpTrack, GdkEventExpose *event, gpointer data)
{
GdkDrawable *window = GTK_LAYOUT(snpTrack)->bin_window;
if (window)
{
GdkDrawable *bitmap = widgetGetDrawable(snpTrack);
if (!bitmap)
{
/* There isn't a bitmap yet. Create it now. */
GtkWidget *detailView = GTK_WIDGET(data);
drawVariationsTrack(snpTrack, detailView);
bitmap = widgetGetDrawable(snpTrack);
}
if (bitmap)
{
/* Push the bitmap onto the window */
GdkGC *gc = gdk_gc_new(window);
gdk_draw_drawable(window, gc, bitmap, 0, 0, 0, 0, -1, -1);
g_object_unref(gc);
}
else
{
g_critical("Failed to draw SNP track [%p] - could not create bitmap.\n", snpTrack);
}
}
return TRUE;
}
/* Handler for when the mouse button is pressed in the SNP track header */
static gboolean onButtonPressSnpTrack(GtkWidget *snpTrack, GdkEventButton *event, gpointer data)
{
gboolean handled = FALSE;
GtkWidget *detailView = GTK_WIDGET(data);
switch (event->button)
{
case 1:
{
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
BlxContext *bc = blxWindowGetContext(blxWindow);
GList *columnList = detailViewGetColumnList(detailView);
if (event->type == GDK_BUTTON_PRESS) /* first click */
{
/* Select the variation that was clicked on. */
blxWindowDeselectAllSeqs(blxWindow);
/* The SNP track is not the same width as the sequence column, so pass the
* sequence column header so that we can convert to the correct coords */
BlxColumnInfo *seqColInfo = getColumnInfo(columnList, BLXCOL_SEQUENCE);
selectClickedSnp(snpTrack, seqColInfo->headerWidget, detailView, event->x, event->y, TRUE, UNSET_INT); /* SNPs are always expanded in the SNP track */
detailViewRefreshAllHeaders(detailView);
}
else if (event->type == GDK_2BUTTON_PRESS) /* double-click */
{
/* If a variation was double-clicked, open its URL in a browser.
* If multiple are selected, use the last-selected one. */
GList *seqItem = g_list_last(blxWindowGetSelectedSeqs(blxWindow));
if (seqItem)
{
BlxSequence *seq = (BlxSequence*)(seqItem->data);
UserFetch *user_fetch = new UserFetch(seq, TRUE, blxWindow, NULL,
#ifdef PFETCH_HTML
bc->ipresolve, bc->cainfo,
#endif
bc->fetch_debug);
user_fetch->performFetch();
}
}
handled = TRUE;
break;
}
}
if (!handled)
{
propagateEventButton(snpTrack, detailView, event);
}
return handled;
}
/* Parent handler for button presses anywhere on the detail view. */
static gboolean onButtonPressDetailView(GtkWidget *detailView, GdkEventButton *event, gpointer data)
{
gboolean handled = FALSE;
const gboolean shiftModifier = (event->state & GDK_SHIFT_MASK) == GDK_SHIFT_MASK;
switch (event->button)
{
case 2:
{
/* Middle button: select the base index at the clicked coords */
/* First make sure the detail view trees are fully drawn (so that the
* cached drawable is up to date - see notes in onExposeDetailViewTree)
* and then set the mouse-drag flag. */
detailViewRedrawAll(detailView);
gdk_window_process_all_updates();
setMouseDragMode(TRUE);
int baseIdx = getBaseIndexAtDetailViewCoords(detailView, event->x, event->y);
if (baseIdx != UNSET_INT)
{
/* For protein matches, select the first base in the triplet */
const int baseNum = 1;
const int frame = detailViewGetActiveFrame(detailView);
detailViewSetSelectedDisplayIdx(detailView, baseIdx, frame, baseNum, FALSE, TRUE, shiftModifier);
}
handled = TRUE;
break;
}
case 3:
{
/* Right button: store the clicked coord (required for copying ref seq),
* and then show the context menu. */
int baseIdx = getBaseIndexAtDetailViewCoords(detailView, event->x, event->y);
if (baseIdx != UNSET_INT)
{
const int baseNum = 1;
const int frame = detailViewGetActiveFrame(detailView);
detailViewSetClickedBaseIdx(detailView, baseIdx, frame, baseNum, FALSE, TRUE);
}
GtkWidget *mainmenu = blxWindowGetMainMenu(detailViewGetBlxWindow(detailView));
gtk_menu_popup (GTK_MENU(mainmenu), NULL, NULL, NULL, NULL, event->button, event->time);
handled = TRUE;
break;
}
default:
break;
};
return handled;
}
static gboolean onMouseMoveDetailView(GtkWidget *detailView, GdkEventMotion *event, gpointer data)
{
gboolean handled = FALSE;
const gboolean shiftModifier = (event->state & GDK_SHIFT_MASK) == GDK_SHIFT_MASK;
if (event->state & GDK_BUTTON2_MASK)
{
/* Moving mouse with middle mouse button down. Update the currently-selected base
* (but don't re-centre on the selected base until the mouse button is released). */
const int baseIdx = getBaseIndexAtDetailViewCoords(detailView, event->x, event->y);
if (baseIdx != UNSET_INT)
{
/* For protein matches, get the 1st base in the peptide */
const int baseNum = 1;
const int frame = detailViewGetActiveFrame(detailView);
/* If shift is pressed, extend the current selection range */
detailViewSetSelectedDisplayIdx(detailView, baseIdx, frame, baseNum, FALSE, TRUE, shiftModifier);
}
handled = TRUE;
}
return handled;
}
/* Implement custom scrolling for horizontal mouse wheel movements over the detailview.
* This scrolls our custom horizontal scrollbar for the whole Detail View. Leaves
* vertical scrolling to the default handler. */
static gboolean onScrollDetailView(GtkWidget *detailView, GdkEventScroll *event, gpointer data)
{
gboolean handled = FALSE;
switch (event->direction)
{
case GDK_SCROLL_LEFT:
{
scrollDetailViewLeftStep(detailView);
handled = TRUE;
break;
}
case GDK_SCROLL_RIGHT:
{
scrollDetailViewRightStep(detailView);
handled = TRUE;
break;
}
default:
{
/* Default handler can handle vertical scrolling */
handled = FALSE;
break;
}
};
return handled;
}
static gboolean onButtonReleaseDetailView(GtkWidget *detailView, GdkEventButton *event, gpointer data)
{
gboolean handled = FALSE;
/* Right button: show context menu (handled in button-press event) */
if (event->button == 3)
{
handled = TRUE;
}
/* Middle button: scroll the selected base index to the centre (unless CTRL is pressed) */
if (event->button == 2)
{
/* Cancel middle-drag mode */
setMouseDragMode(FALSE);
const gboolean ctrlModifier = (event->state & GDK_CONTROL_MASK) == GDK_CONTROL_MASK;
if (!ctrlModifier)
{
/* Move the scrollbar so that the currently-selected base index is at the centre */
const gboolean selectedBaseSet = detailViewGetSelectedIdxSet(detailView);
const int selectedBaseIdx = detailViewGetSelectedDisplayIdx(detailView);
if (selectedBaseSet)
{
/* The coord is in terms of the display coords, i.e. whatever the displayed seq type is. */
const BlxSeqType seqType = detailViewGetSeqType(detailView);
const IntRange* const displayRange = detailViewGetDisplayRange(detailView);
int newStart = selectedBaseIdx - (displayRange->length() / 2);
setDetailViewStartIdx(detailView, newStart, seqType);
}
}
detailViewRedrawAll(detailView);
handled = TRUE;
}
return handled;
}
/* Show the detail-view-header context menu, if applicable (return false if not) */
static gboolean detailViewHeaderShowContextMenu(GtkWidget *header, GtkWidget *detailView, GdkEventButton *event)
{
gboolean handled = FALSE;
/* Check if the click is in the selected coord range and if so show the sequence-header menu */
if (detailViewGetSelectedIdxSet(detailView))
{
const int displayIdx = seqColHeaderGetCoordAtPos(header, detailView, event->x, event->y);
IntRange *range = detailViewGetSelectedDisplayIdxRange(detailView);
if (valueWithinRange(displayIdx, range))
{
GtkWidget *blxWindow = detailViewGetBlxWindow(detailView);
GtkWidget *menu = blxWindowGetSeqHeaderMenu(blxWindow);
gtk_menu_popup (GTK_MENU(menu), NULL, NULL, NULL, NULL, event->button, event->time);
handled = TRUE;
}
}
return handled;
}
/* Handler for when a mouse button is pressed on a sequence column header widget.
* This signal only gets connected for protein matches, where there are 3 header
* widgets, each showing the DNA nucleotides for a specific reading frame. */
static gboolean onButtonPressSeqColHeader(GtkWidget *header, GdkEventButton *event, gpointer data)
{
gboolean handled = FALSE;
const gboolean shiftModifier = (event->state & GDK_SHIFT_MASK) == GDK_SHIFT_MASK;
GtkWidget *detailView = GTK_WIDGET(data);
switch (event->button)
{
case 1:
{
if (event->type == GDK_BUTTON_PRESS)
{
/* Select the SNP that was clicked on. */
blxWindowDeselectAllSeqs(detailViewGetBlxWindow(detailView));
const int clickedBase = seqColHeaderGetBase(header, 1, detailViewGetNumFrames(detailView));
selectClickedSnp(header, NULL, detailView, event->x, event->y, FALSE, clickedBase); /* SNPs are always un-expanded in the DNA track */
detailViewRefreshAllHeaders(detailView);
}
else if (event->type == GDK_2BUTTON_PRESS)
{
/* Double-click: toggle variations-track visibility */
BlxContext *bc = detailViewGetContext(detailView);
const gboolean showTrack = !bc->flags[BLXFLAG_SHOW_VARIATION_TRACK];
/* If we're enabling the variations track, also make sure that variations are highlighted in the header */
if (showTrack)
bc->flags[BLXFLAG_HIGHLIGHT_VARIATIONS] = TRUE;
bc->flags[BLXFLAG_SHOW_VARIATION_TRACK] = showTrack;
detailViewUpdateShowSnpTrack(detailView, showTrack);
}
handled = TRUE;
break;
}
case 2:
{
/* Middle button: select the nucleotide that was clicked on. */
selectClickedNucleotide(header, detailView, event->x, event->y, shiftModifier);
handled = TRUE;
break;
}
case 3: /* right button */
{
handled = detailViewHeaderShowContextMenu(header, detailView, event);
break;
}
default:
break;
}
if (!handled)
{
propagateEventButton(header, detailView, event);
}
return handled;
}
static gboolean onButtonReleaseSeqColHeader(GtkWidget *header, GdkEventButton *event, gpointer data)
{
gboolean handled = FALSE;
/* Middle button: scroll the selected base index to the centre (unless CTRL is pressed) */
if (event->button == 2)
{
const gboolean ctrlModifier = (event->state & GDK_CONTROL_MASK) == GDK_CONTROL_MASK;
if (!ctrlModifier)
{
/* Move the scrollbar so that the currently-selected base index is at the centre */
GtkWidget *detailView = GTK_WIDGET(data);
detailViewCentreOnSelection(detailView);
}
handled = TRUE;
}
return handled;
}
static gboolean onMouseMoveSeqColHeader(GtkWidget *header, GdkEventMotion *event, gpointer data)
{
GtkWidget *detailView = GTK_WIDGET(data);
const gboolean shiftModifier = (event->state & GDK_SHIFT_MASK) == GDK_SHIFT_MASK;
if (event->state & GDK_BUTTON2_MASK)
{
/* Moving mouse with middle mouse button down. Update the currently-selected base */
selectClickedNucleotide(header, detailView, event->x, event->y, shiftModifier);
}
else
{
/* Get the nucelotide we're hovering over and feedback info about that nucelotide in the
* feedback area */
int baseIdx, frame, baseNum;
getSeqColHeaderClickedNucleotide(header, detailView, event->x, event->y, &baseIdx, &frame, &baseNum);
if (baseIdx != UNSET_INT && frame != UNSET_INT && baseNum != UNSET_INT)
{
BlxContext *bc = detailViewGetContext(detailView);
const int dnaIdx = convertDisplayIdxToDnaIdx(baseIdx, bc->seqType, frame, baseNum, bc->numFrames, bc->displayRev, &bc->refSeqRange);
const BlxStrand strand = blxWindowGetActiveStrand(detailViewGetBlxWindow(detailView));
updateFeedbackAreaNucleotide(detailView, dnaIdx, strand);
}
}
return TRUE;
}
/* Updates the widths of any dynamic-sized columns (really they are fixed-width
* columns so we update their widths manually here). */
void updateDynamicColumnWidths(GtkWidget *detailView)
{
/* Currently, only the sequence column has dynamic width, so just sum all of the
* other (visible) column widths and subtract from the allocation width. */
int width = detailView->allocation.width;
BlxColumnInfo *seqColInfo = NULL;
GList *listItem = detailViewGetColumnList(detailView);
for ( ; listItem; listItem = listItem->next)
{
BlxColumnInfo *columnInfo = (BlxColumnInfo*)(listItem->data);
if (columnInfo->columnId == BLXCOL_SEQUENCE)
seqColInfo = columnInfo;
else if (columnInfo->showColumn && columnInfo->dataLoaded)
width -= columnInfo->width;
}
if (seqColInfo && seqColInfo->width != width)
{
seqColInfo->width = width;
callFuncOnAllDetailViewTrees(detailView, resizeTreeColumns, NULL);
resizeDetailViewHeaders(detailView);
updateDetailViewRange(detailView);
}
}
/***********************************************************
* Callbacks *
***********************************************************/
/* If there are two trees and one is visible and the other hidden, toggle their hidden states */
static void swapTreeVisibility(GtkWidget *detailView)
{
/* Only do anything for DNA matches, where we have 1 frame and both strands are visible. */
if (detailViewGetSeqType(detailView) == BLXSEQ_DNA)
{
GtkWidget *tree1 = detailViewGetTreeContainer(detailView, BLXSTRAND_FORWARD, 1);
GtkWidget *tree2 = detailViewGetTreeContainer(detailView, BLXSTRAND_REVERSE, 1);
if (widgetGetHidden(tree1) != widgetGetHidden(tree2))
{
widgetSetHidden(tree1, !widgetGetHidden(tree1));
widgetSetHidden(tree2, !widgetGetHidden(tree2));
}
}
}
/* If one grid is visible and the other hidden, toggle their hidden states */
static void swapGridVisibility(GtkWidget *bigPicture)
{
GtkWidget *grid1 = bigPictureGetFwdGrid(bigPicture);
GtkWidget *grid2 = bigPictureGetRevGrid(bigPicture);
if (widgetGetHidden(grid1) != widgetGetHidden(grid2))
{
widgetSetHidden(grid1, !widgetGetHidden(grid1));
widgetSetHidden(grid2, !widgetGetHidden(grid2));
}
}
/* If one exon view is visible and the other hidden, toggle their hidden states */
static void swapExonViewVisibility(GtkWidget *bigPicture)
{
GtkWidget *exonView1 = bigPictureGetFwdExonView(bigPicture);
GtkWidget *exonView2 = bigPictureGetRevExonView(bigPicture);
if (widgetGetHidden(exonView1) != widgetGetHidden(exonView2))
{
widgetSetHidden(exonView1, !widgetGetHidden(exonView1));
widgetSetHidden(exonView2, !widgetGetHidden(exonView2));
}
}
void toggleStrand(GtkWidget *detailView)
{
DEBUG_ENTER("toggleStrand");
BlxContext *blxContext = detailViewGetContext(detailView);
GtkWidget *bigPicture = detailViewGetBigPicture(detailView);
/* Update the flag */
blxContext->displayRev = !blxContext->displayRev;
/* Invert the display range for both detail view and big picture */
IntRange *bpRange = bigPictureGetDisplayRange(bigPicture);
const IntRange* const fullRange = &blxContext->fullDisplayRange;
const int bpStart = fullRange->max() - bpRange->min() + fullRange->min();
const int bpEnd = fullRange->max() - bpRange->max() + fullRange->min();
bpRange->set(bpStart, bpEnd);
IntRange *displayRange = detailViewGetDisplayRange(detailView);
const int newStart = fullRange->max() - displayRange->max() + fullRange->min();
setDetailViewStartIdx(detailView, newStart, blxContext->seqType);
/* Re-select the currently-selected index/range, if set, because the display coords
* have changed and need updating in the index structs. */
DetailViewProperties *properties = detailViewGetProperties(detailView);
detailViewRefreshSelection(detailView);
/* Re-calculate the cached display ranges for the MSPs */
cacheMspDisplayRanges(blxContext, properties->numUnalignedBases);
/* Update the feedback box */
updateFeedbackBox(detailView);
/* If one grid/tree is hidden and the other visible, toggle which is hidden */
swapTreeVisibility(detailView);
swapGridVisibility(bigPicture);
swapExonViewVisibility(bigPicture);
/* Toggle the order of the trees and grids. */
refreshTreeOrder(detailView);
refreshGridOrder(bigPicture);
/* Redraw the tree and detail-view headers. Also need to resort because if the display is
* reversed it affects sorting by position. */
refreshDetailViewHeaders(detailView);
detailViewResortTrees(detailView);
callFuncOnAllDetailViewTrees(detailView, refreshTreeHeaders, NULL);
callFuncOnAllDetailViewTrees(detailView, refilterTree, NULL);
detailViewRedrawAll(detailView);
/* Redraw the grids and grid headers */
refreshBigPictureDisplayRange(bigPicture, FALSE);
DEBUG_EXIT("toggleStrand returning");
}
/* Go to a user-specified coord on the reference sequence (in terms of the DNA
* or peptide sequence as specified by coordSeqType). Selects the base index
* and centres on it. */
void goToDetailViewCoord(GtkWidget *detailView, const BlxSeqType coordSeqType)
{
static gchar defaultInput[32] = "";
static gboolean toplevelCoords = FALSE;
BlxContext *bc = detailViewGetContext(detailView);
/* Pop up a dialog to request a coord from the user */
char *title = g_strdup_printf("%sGo to position", blxGetTitlePrefix(bc));
GtkWidget *dialog = gtk_dialog_new_with_buttons(title,
NULL,
(GtkDialogFlags)(GTK_DIALOG_MODAL | GTK_DIALOG_DESTROY_WITH_PARENT | GTK_DIALOG_NO_SEPARATOR),
GTK_STOCK_OK, GTK_RESPONSE_ACCEPT,
GTK_STOCK_CANCEL, GTK_RESPONSE_REJECT,
NULL);
g_free(title);
gtk_dialog_set_default_response(GTK_DIALOG(dialog), GTK_RESPONSE_ACCEPT);
GtkWidget *contentArea = GTK_DIALOG(dialog)->vbox;
const int padding = 4;
/* Create a text-entry widget for the user to enter the coord */
GtkWidget *hbox = gtk_hbox_new(FALSE, 0);
gtk_box_pack_start(GTK_BOX(contentArea), hbox, FALSE, FALSE, padding);
GtkWidget *label = gtk_label_new("Enter coord: ");
gtk_box_pack_start(GTK_BOX(hbox), label, FALSE, FALSE, padding);
GtkWidget *entry = gtk_entry_new();
gtk_entry_set_text(GTK_ENTRY(entry), defaultInput);
gtk_entry_set_activates_default(GTK_ENTRY(entry), TRUE);
gtk_box_pack_start(GTK_BOX(hbox), entry, TRUE, TRUE, padding);
/* Create a tick-box to toggle to top-level coords (default is local coords) */
GtkWidget *checkBox = gtk_check_button_new_with_mnemonic("Use _top-level coords");
gtk_toggle_button_set_active(GTK_TOGGLE_BUTTON(checkBox), toplevelCoords);
gtk_box_pack_start(GTK_BOX(contentArea), checkBox, FALSE, FALSE, padding);
/* Show the dialog and wait for a response */
gtk_widget_show_all(dialog);
if (gtk_dialog_run(GTK_DIALOG(dialog)) == GTK_RESPONSE_ACCEPT)
{
const gchar *inputText = gtk_entry_get_text(GTK_ENTRY(entry));
toplevelCoords = gtk_toggle_button_get_active(GTK_TOGGLE_BUTTON(checkBox));
/* Convert the input string to an int */
int requestedCoord = atoi(inputText);
if (requestedCoord)
{
/* Remember this input for next time */
sprintf(defaultInput, "%d", requestedCoord);
int coord = requestedCoord;
/* If display coords are negated, assume the user has entered a
* negative coord too, and un-negate it. */
const gboolean negate = negateCoords(bc);
if (negate)
{
coord *= -1;
}
/* If the user entered a top-level coord, apply the offset to convert to local coords */
if (toplevelCoords)
{
coord += bc->refSeqOffset;
}
/* Check if it's in range. If not, try negating it in case the user
* entered the wrong sign */
if (!valueWithinRange(coord, &bc->refSeqRange))
{
g_debug("Coord '%d' does not lie within the reference sequence range [%d %d]; trying '%d'\n", requestedCoord, negate ? bc->refSeqRange.max() * -1 : bc->refSeqRange.min(), negate ? bc->refSeqRange.min() * -1 : bc->refSeqRange.max(), requestedCoord * -1);
coord *= -1;
}
if (valueWithinRange(coord, &bc->refSeqRange))
{
const int activeFrame = detailViewGetActiveFrame(detailView);
detailViewSetSelectedDnaBaseIdx(detailView, coord, activeFrame, TRUE, FALSE, FALSE);
detailViewRedrawAll(detailView);
}
else
{
g_debug("Coord '%d' does not lie within the reference sequence range [%d %d]\n", negate ? coord * -1 : coord, negate? bc->refSeqRange.max() * -1 : bc->refSeqRange.min(), negate ? bc->refSeqRange.min() * -1 : bc->refSeqRange.max());
g_critical("Coord was not in the reference sequence range [%d %d]\n", negate ? bc->refSeqRange.max() * -1 : bc->refSeqRange.min(), negate ? bc->refSeqRange.min() * -1 : bc->refSeqRange.max());
}
}
}
gtk_widget_destroy(dialog);
}
/* This sets the main sort column to be the given column (it does not change
* any secondary sort columns). */
void detailViewSetSortColumn(GtkWidget *detailView, const BlxColumnId sortColumn)
{
DetailViewProperties *properties = detailViewGetProperties(detailView);
GList *columnList = blxWindowGetColumnList(properties->blxWindow());
const int numColumns = g_list_length(columnList);
if (numColumns > 0)
{
if (properties->sortColumns[0] != sortColumn)
{
properties->sortColumns[0] = sortColumn;
detailViewResortTrees(detailView);
}
}
}
/* Find the next MSP (out the MSPs in this tree) whose start/end is the next closest to the
* current start position of the display, searching only in the direction specified by the
* search criteria passed in the user data. Updates the searchData with the offset found. */
static gboolean findNextMatchInTree(GtkTreeModel *model, GtkTreePath *path, GtkTreeIter *iter, gpointer data)
{
MatchSearchData *searchData = (MatchSearchData*)data;
/* Loop through all MSPs in this tree row. */
GList* mspListItem = treeGetMsps(model, iter);
for ( ; mspListItem; mspListItem = mspListItem->next)
{
MSP *msp = (MSP*)(mspListItem->data);
/* Check its in the sequence list (if given), is a valid match or exon, and is in a visible layer */
if (mspLayerIsVisible(msp) &&
(mspIsBoxFeature(msp) || mspIsBlastMatch(msp)) &&
(!searchData->seqList || g_list_find(searchData->seqList, msp->sSequence)))
{
/* Get the offset of the msp coords from the given start coord and find the smallest,
* ignorning zero and negative offsets (negative means its the wrong direction) */
const int offset1 = (msp->qRange.min() - searchData->startDnaIdx) * searchData->searchDirection;
const int offset2 = (msp->qRange.max() - searchData->startDnaIdx) * searchData->searchDirection;
int currentFrame = mspGetRefFrame(msp, searchData->seqType);
int currentBest = UNSET_INT;
if (offset1 > 0 && (offset2 <= 0 || offset2 >= offset1))
{
currentBest = offset1;
}
else
{
currentBest = offset2;
}
if (currentBest > 0)
{
gboolean useNew = FALSE;
/* Check if this is the first one we've looked at */
useNew |= (searchData->offset == UNSET_INT);
/* Check if this offset is smaller than the previous best */
useNew |= (currentBest < searchData->offset);
/* If it's the same as the previous best, use the one with the smallest
* frame number, i.e. give preference to the topmost frame (in the hope
* that this will be the least confusing!) */
useNew |= (currentBest == searchData->offset && currentFrame < searchData->foundFrame);
if (useNew)
{
searchData->offset = currentBest;
searchData->foundFrame = currentFrame;
searchData->foundMsp = msp;
}
}
}
}
return FALSE;
}
/* Find and go to the next match (either left or right depending on the search flag).
* If a list of sequence names is given, only look at matches in those sequences.
* startDnaIdx determines where to start searching from. Sets the found match idx as
* the currently-selected base index.
* If extend is true, extend the current selection range to the next match position */
static MSP* goToNextMatch(GtkWidget *detailView,
const int startDnaIdx,
const gboolean searchRight,
GList *seqList,
const gboolean extend)
{
BlxContext *bc = detailViewGetContext(detailView);
const int searchDirection = (searchRight != bc->displayRev) ? 1 : -1;
MatchSearchData searchData = {startDnaIdx,
searchRight,
searchDirection,
bc->displayRev,
bc->seqType,
bc->numFrames,
&bc->refSeqRange,
seqList,
UNSET_INT,
UNSET_INT,
NULL};
/* Loop through the MSPs in all visible trees */
int frame = 1;
for ( ; frame <= searchData.numFrames; ++frame)
{
GtkWidget *treeContainer = detailViewGetTreeContainer(detailView, BLXSTRAND_FORWARD, frame);
GtkWidget *tree = treeContainerGetTree(GTK_CONTAINER(treeContainer));
if (GTK_WIDGET_VISIBLE(tree) && gtk_widget_get_parent(treeContainer)) /* ignore if not currently included in view */
{
GtkTreeModel *model = treeGetBaseDataModel(GTK_TREE_VIEW(tree));
gtk_tree_model_foreach(model, findNextMatchInTree, &searchData);
}
treeContainer = detailViewGetTreeContainer(detailView, BLXSTRAND_REVERSE, frame);
tree = treeContainerGetTree(GTK_CONTAINER(treeContainer));
if (GTK_WIDGET_VISIBLE(tree) && gtk_widget_get_parent(treeContainer)) /* ignore if not currently included in view */
{
GtkTreeModel *model = treeGetBaseDataModel(GTK_TREE_VIEW(tree));
gtk_tree_model_foreach(model, findNextMatchInTree, &searchData);
}
}
if (searchData.offset != UNSET_INT)
{
/* Offset the start coord by the found amount. */
int newDnaIdx = searchData.startDnaIdx + (searchData.offset * searchDirection);
detailViewSetSelectedDnaBaseIdx(detailView, newDnaIdx, searchData.foundFrame, TRUE, FALSE, extend);
callFuncOnAllDetailViewTrees(detailView, treeScrollSelectionIntoView, NULL);
detailViewRedrawAll(detailView);
}
return searchData.foundMsp;
}
/* Go to the previous match (optionally limited to matches in the given list) */
MSP* prevMatch(GtkWidget *detailView, GList *seqList, const gboolean extend)
{
/* Jump to the nearest match to the currently selected base index, if there is
* one and if it is currently visible. Otherwise use the current display centre. */
int startDnaIdx = detailViewGetSelectedDnaIdx(detailView);
int startCoord = detailViewGetSelectedDisplayIdx(detailView);
const IntRange* const displayRange = detailViewGetDisplayRange(detailView);
if (!valueWithinRange(startCoord, displayRange))
{
startCoord = displayRange->centre();
/* Use base 1 within the currently selected frame for this display coord */
int frame = detailViewGetActiveFrame(detailView);
BlxContext *bc = detailViewGetContext(detailView);
startDnaIdx = convertDisplayIdxToDnaIdx(startCoord, bc->seqType, frame, 1, bc->numFrames, bc->displayRev, &bc->refSeqRange);
}
return goToNextMatch(detailView, startDnaIdx, FALSE, seqList, extend);
}
/* Go to the next match (optionally limited to matches in the given list) */
MSP* nextMatch(GtkWidget *detailView, GList *seqList, const gboolean extend)
{
/* Jump to the nearest match to the currently selected base index, if there is
* one and if it is currently visible. Otherwise use the current display centre. */
int startDnaIdx = detailViewGetSelectedDnaIdx(detailView);
int startCoord = detailViewGetSelectedDisplayIdx(detailView);
const IntRange* const displayRange = detailViewGetDisplayRange(detailView);
if (!valueWithinRange(startCoord, displayRange))
{
startCoord = displayRange->centre();
/* Use base 1 within the currently selected frame for this display coord */
int frame = detailViewGetActiveFrame(detailView);
BlxContext *bc = detailViewGetContext(detailView);
startDnaIdx = convertDisplayIdxToDnaIdx(startCoord, bc->seqType, frame, 1, bc->numFrames, bc->displayRev, &bc->refSeqRange);
}
return goToNextMatch(detailView, startDnaIdx, TRUE, seqList, extend);
}
/* Go to the first match (optionally limited to matches in the given list) */
MSP* firstMatch(GtkWidget *detailView, GList *seqList, const gboolean extend)
{
/* Jump to the nearest match to the start of the ref seq */
const IntRange* const refSeqRange = detailViewGetRefSeqRange(detailView);
const int startIdx = detailViewGetDisplayRev(detailView) ? refSeqRange->max() : refSeqRange->min();
return goToNextMatch(detailView, startIdx, TRUE, seqList, extend);
}
/* Go to the last match (optionally limited to matches in the given list) */
MSP* lastMatch(GtkWidget *detailView, GList *seqList, const gboolean extend)
{
/* Jump to the nearest match to the end of the reference sequence */
const IntRange* const refSeqRange = detailViewGetRefSeqRange(detailView);
const int startIdx = detailViewGetDisplayRev(detailView) ? refSeqRange->min() : refSeqRange->max();
return goToNextMatch(detailView,
startIdx, FALSE, seqList, extend);
}
/***********************************************************
* Initialization *
***********************************************************/
/* This loops through all the columns and adds each column's header widget to the header bar. */
static void addColumnsToHeaderBar(GtkBox *headerBar, GList *columnList)
{
/* Loop through each column and add its header widget to the header bar */
GList *columnItem = columnList;
for ( ; columnItem; columnItem = columnItem->next)
{
BlxColumnInfo *columnInfo = (BlxColumnInfo*)(columnItem->data);
/* The sequence column is a special one that wants to fill any additional space, so
* set the 'expand' property to true for that column only. */
const gboolean expand = (columnInfo->columnId == BLXCOL_SEQUENCE );
gtk_box_pack_start(headerBar, columnInfo->headerWidget, expand, TRUE, 0);
}
}
/* Create the header bar for the detail view. This contains the labels for the
* detail-view trees (since we only want one label at the top, rather than
* individual labels for each tree). For protein sequence matches, the header
* for the sequence column will also show the DNA sequence (separated into reading
* frames).
* Column data is compiled into the detailViewColumns return argument.
* If includeSnpTrack is true then the snp track widget will also be created. */
static GtkWidget* createDetailViewHeader(GtkWidget *detailView,
const BlxSeqType seqType,
const int numFrames,
GList *columnList,
const gboolean includeSnpTrack,
GtkWidget **snpTrack)
{
GtkBox *headerBar = GTK_BOX(gtk_hbox_new(FALSE, 0)); /* hbox for the column headers */
/* Create a SNP track, if requested */
if (includeSnpTrack && snpTrack)
{
*snpTrack = createSnpTrackHeader(detailView, BLXSTRAND_NONE);
}
/* Add all the column headers to the header bar */
addColumnsToHeaderBar(headerBar, columnList);
return GTK_WIDGET(headerBar);
}
/* Create the variations track header widget. If the strand is BLXSTRAND_NONE
* then the active strand will be used. */
GtkWidget* createSnpTrackHeader(GtkWidget *detailView, const BlxStrand strand)
{
GtkWidget *snpTrack = gtk_layout_new(NULL, NULL);
gtk_widget_set_name(snpTrack, SNP_TRACK_HEADER_NAME);
snpTrackSetStrand(snpTrack, strand);
gtk_widget_add_events(snpTrack, GDK_BUTTON_PRESS_MASK);
g_signal_connect(G_OBJECT(snpTrack), "expose-event", G_CALLBACK(onExposeVariationsTrack), detailView);
g_signal_connect(G_OBJECT(snpTrack), "button-press-event", G_CALLBACK(onButtonPressSnpTrack), detailView);
return snpTrack;
}
/* Create a custom header widget for the sequence column for protein matches (this is where
* we will display the triplets that make up the codons.) Returns NULL for DNA matches. */
static void createSeqColHeader(GtkWidget *detailView,
const BlxSeqType seqType,
const int numFrames,
GList *columnList)
{
if (seqType == BLXSEQ_PEPTIDE)
{
GtkWidget *header = gtk_vbox_new(FALSE, 0);
int frame = 0;
for ( ; frame < numFrames; ++frame)
{
GtkWidget *line = gtk_layout_new(NULL, NULL);
gtk_box_pack_start(GTK_BOX(header), line, FALSE, TRUE, 0);
gtk_widget_set_name(line, DNA_TRACK_HEADER_NAME);
seqColHeaderSetRow(line, frame + 1);
/* Disable double buffering to try to speed up drawing */
GTK_WIDGET_UNSET_FLAGS (line, GTK_DOUBLE_BUFFERED);
gtk_widget_add_events(line, GDK_BUTTON_PRESS_MASK);
gtk_widget_add_events(line, GDK_BUTTON_RELEASE_MASK);
gtk_widget_add_events(line, GDK_POINTER_MOTION_MASK);
g_signal_connect(G_OBJECT(line), "expose-event", G_CALLBACK(onExposeDnaTrack), detailView);
g_signal_connect(G_OBJECT(line), "button-press-event", G_CALLBACK(onButtonPressSeqColHeader), detailView);
g_signal_connect(G_OBJECT(line), "button-release-event", G_CALLBACK(onButtonReleaseSeqColHeader), detailView);
g_signal_connect(G_OBJECT(line), "motion-notify-event", G_CALLBACK(onMouseMoveSeqColHeader), detailView);
}
/* Set the header widget (and its refresh function) in the column data */
BlxColumnInfo* columnInfo = getColumnInfo(columnList, BLXCOL_SEQUENCE);
columnInfo->headerWidget = header;
columnInfo->refreshFunc = refreshTextHeader;
}
}
/* Create the horizontal scrollbar for custom scrolling. This takes ownership of
* the GtkAdjustment. */
GtkWidget* createDetailViewScrollBar(GtkAdjustment *adjustment, GtkWidget *detailView)
{
GtkWidget *scrollBar = gtk_hscrollbar_new(adjustment);
/* Connect signals */
g_signal_connect(G_OBJECT(adjustment), "changed", G_CALLBACK(onScrollRangeChangedDetailView), detailView);
g_signal_connect(G_OBJECT(adjustment), "value-changed", G_CALLBACK(onScrollPosChangedDetailView), detailView);
return scrollBar;
}
static void createFeedbackBoxEntry(GtkBox *parent,
const char *widget_name,
const char *tooltip,
GCallback cb_func,
gpointer cb_data)
{
const int charWidth = 8; /* guesstimate */
GtkWidget *entry = gtk_entry_new() ;
gtk_widget_set_name(entry, widget_name);
gtk_widget_set_tooltip_text(entry, tooltip);
gtk_editable_set_editable(GTK_EDITABLE(entry), FALSE);
gtk_widget_set_size_request(entry, DETAIL_VIEW_FEEDBACK_MIN_WIDTH * charWidth, -1) ;
gtk_box_pack_start(GTK_BOX(parent), entry, FALSE, FALSE, 0);
/* We want the box to be printed, so connect the expose function that will
* draw to a pixmap for printing */
g_signal_connect(G_OBJECT(entry), "expose-event", cb_func, cb_data);
}
/* Create the feedback box. (This is actually a set of several boxes which feed
* back info to the user about the currently-selected base/sequence.) */
static GtkWidget* createFeedbackBox(GtkToolbar *toolbar, char *windowColor)
{
/* Put all feedback boxes into a parent hbox */
GtkWidget *feedbackBox = gtk_hbox_new(FALSE, 0);
GtkBox *box = GTK_BOX(feedbackBox);
blxSetWidgetColor(feedbackBox, windowColor);
/* Reference sequence coord */
createFeedbackBoxEntry(box, DETAIL_VIEW_FEEDBACK_REF_COORD, "Reference sequence coord(s)",
G_CALLBACK(onExposePrintable), NULL);
/* Match sequence "name:coord/len" */
gtk_box_pack_start(box, gtk_label_new(" "), FALSE, FALSE, 0);
createFeedbackBoxEntry(box, DETAIL_VIEW_FEEDBACK_MATCH_NAME, DETAIL_VIEW_FEEDBACK_MATCH_NAME_TOOLTIP,
G_CALLBACK(onExposePrintable), NULL);
gtk_box_pack_start(box, gtk_label_new(":"), FALSE, FALSE, 0);
createFeedbackBoxEntry(box, DETAIL_VIEW_FEEDBACK_MATCH_COORD, "Match sequence coord(s)",
G_CALLBACK(onExposePrintable), NULL);
gtk_box_pack_start(box, gtk_label_new("/"), FALSE, FALSE, 0);
createFeedbackBoxEntry(box, DETAIL_VIEW_FEEDBACK_MATCH_LEN, "Match sequence length",
G_CALLBACK(onExposePrintable), NULL);
/* Read depth at selected coord(s) */
gtk_box_pack_start(box, gtk_label_new(" "), FALSE, FALSE, 0);
createFeedbackBoxEntry(box, DETAIL_VIEW_FEEDBACK_DEPTH, DETAIL_VIEW_FEEDBACK_DEPTH_TOOLTIP,
G_CALLBACK(onExposePrintable), NULL);
return feedbackBox;
}
/* Create the status bar for the detail-view toolbar. (This feeds back info to the user
* about the currently-moused-over sequence.) */
static GtkWidget* createStatusBar(GtkToolbar *toolbar, char *windowColor)
{
GtkWidget *statusBar = gtk_statusbar_new() ;
gtk_statusbar_set_has_resize_grip(GTK_STATUSBAR(statusBar), FALSE);
blxSetWidgetColor(statusBar, windowColor);
/* Make it expandable so we use all available space. Set minimum size to be 0
* because it's better to show it small than not at all. */
gtk_widget_set_size_request(statusBar, 0, -1) ;
//GtkToolItem *item = addToolbarWidget(toolbar, statusBar, 0) ;
//gtk_tool_item_set_expand(item, TRUE); /* make as big as possible */
setStatusBarShadowStyle(statusBar, "GTK_SHADOW_NONE");
return statusBar;
}
/* Create the detail view toolbar */
static GtkWidget* createDetailViewButtonBar(GtkWidget *detailView,
GtkWidget *toolbarIn,
BlxBlastMode mode,
const BlxColumnId sortColumn,
GList *columnList,
char *windowColor,
GtkWidget **feedbackBox,
GtkWidget **statusBar)
{
GtkToolbar *toolbar = GTK_TOOLBAR(toolbarIn);
blxSetWidgetColor(GTK_WIDGET(toolbar), windowColor);
gtk_toolbar_set_style(toolbar, GTK_TOOLBAR_ICONS);
gtk_toolbar_set_icon_size(toolbar, GTK_ICON_SIZE_SMALL_TOOLBAR);
*feedbackBox = createFeedbackBox(toolbar, windowColor);
*statusBar = createStatusBar(toolbar, windowColor);
/* Create a parent hbox which will hold the main toolbar
* and the detail-view tools. (We do this rather than putting
* the detail-view tools directly into the toolbar so that
* we have more control over spacing. In particular, the
* toolbar tools go into a nice drop-down box when there isn't
* enough space, but the detail-view tools won't display if
* that happens, so we keep them separate.) */
GtkBox *hbox = GTK_BOX(gtk_hbox_new(FALSE, 0));
gtk_box_pack_start(hbox, toolbarIn, TRUE, TRUE, 0);
gtk_box_pack_start(hbox, *feedbackBox, FALSE, FALSE, 0);
gtk_box_pack_start(hbox, *statusBar, TRUE, TRUE, 0);
/* Put the toolbar in a handle box so that it can be torn off */
GtkWidget *toolbarContainer = createToolbarHandle();
gtk_container_add(GTK_CONTAINER(toolbarContainer), GTK_WIDGET(hbox));
blxSetWidgetColor(toolbarContainer, windowColor);
return toolbarContainer;
}
/* Create two detail-view trees and place them in the 2 panes of the container (if one is
* given). The first tree gets associated with grid1 and appended to list1, and the second
* with grid2 and list2. If hasHeaders is true, the first tree will have visible headers. */
static void createTwoPanedTrees(GtkWidget *detailView,
GtkPaned *panedWin,
GtkCellRenderer *renderer,
GtkWidget *grid1,
GtkWidget *grid2,
GList **list1,
GList **list2,
BlxSeqType seqType,
GList *columnList,
const char* const refSeqName,
const int frame1,
const int frame2,
const gboolean includeSnpTrack)
{
GtkWidget *tree1 = createDetailViewTree(grid1, detailView, renderer, list1, columnList, seqType, refSeqName, frame1, includeSnpTrack);
GtkWidget *tree2 = createDetailViewTree(grid2, detailView, renderer, list2, columnList, seqType, refSeqName, frame2, includeSnpTrack);
if (panedWin)
{
gtk_paned_pack1(GTK_PANED(panedWin), tree1, TRUE, TRUE);
gtk_paned_pack2(GTK_PANED(panedWin), tree2, TRUE, TRUE);
}
}
/* Create three detail-view trees and place them in the paned widget. We only have
* two panes in the widget, so two of the trees will be placed in a second, nested
* paned widget. */
static void createThreePanedTrees(GtkWidget *detailView,
GtkPaned *panedWin,
GtkCellRenderer *renderer,
GtkWidget *grid,
GList **list,
BlxSeqType seqType,
const gboolean addToDetailView,
GList *columnList,
const char* const refSeqName,
const gboolean includeSnpTrack)
{
const int frame1 = 1, frame2 = 2, frame3 = 3;
/* Create a tree for pane1 (but only add it to the detailView if instructed to).
* The first tree has headers. */
GtkWidget *tree1 = createDetailViewTree(grid, detailView, renderer, list, columnList, seqType, refSeqName, frame1, includeSnpTrack);
GtkPaned *nestedPanedWidget = NULL;
if (addToDetailView)
{
gtk_paned_pack1(GTK_PANED(panedWin), tree1, TRUE, TRUE);
/* Create another paned widget and place it in pane 2 */
nestedPanedWidget = GTK_PANED(gtk_vpaned_new());
gtk_paned_pack2(panedWin, GTK_WIDGET(nestedPanedWidget), TRUE, TRUE);
}
/* Create two more trees (and place them in the nested paned widget, if it is not null).
* Neither of these trees should have headers. */
createTwoPanedTrees(detailView, nestedPanedWidget, renderer, grid, grid, list, list, seqType, columnList, refSeqName, frame2, frame3, includeSnpTrack);
}
/* Create the trees for the detail view, creating sub-panes if necessary depending
* on how many trees we need */
static void createDetailViewPanes(GtkWidget *detailView,
GtkPaned *panedWin,
GtkCellRenderer *renderer,
GtkWidget *fwdStrandGrid,
GtkWidget *revStrandGrid,
const int numFrames,
GList **fwdStrandTrees,
GList **revStrandTrees,
BlxSeqType seqType,
GList *columnList,
const char* const refSeqName,
const gboolean includeSnpTrack)
{
if (numFrames == 1)
{
/* DNA matches: we need 2 trees, one for the forward strand and one for the reverse. */
createTwoPanedTrees(detailView,
panedWin,
renderer,
fwdStrandGrid,
revStrandGrid,
fwdStrandTrees,
revStrandTrees,
seqType,
columnList,
refSeqName,
1,
1,
includeSnpTrack);
}
else if (numFrames == 3)
{
/* Protein matches: we need 3 trees for the 3 reading frames for EACH strand (although only
* one set of trees will be displayed at a time). */
createThreePanedTrees(detailView, panedWin, renderer, fwdStrandGrid, fwdStrandTrees, seqType, TRUE, columnList, refSeqName, includeSnpTrack);
createThreePanedTrees(detailView, panedWin, renderer, revStrandGrid, revStrandTrees, seqType, FALSE, columnList, refSeqName, includeSnpTrack);
}
}
/***********************************************************
* Initialization *
***********************************************************/
/* Add the MSPs to the detail-view trees. Calculates and returns the lowest ID
* out of all the blast matches. modelId specifies which tree data model should
* be active at the start. If create is true, the tree data stores are created;
* otherwise we assume they already exist. */
void detailViewAddMspData(GtkWidget *detailView, MSP *mspList, GList *seqList)
{
BlxContext *bc = detailViewGetContext(detailView);
/* First, make sure there is a data model for each tree */
callFuncOnAllDetailViewTrees(detailView, treeCreateBaseDataModel, NULL);
/* Loop through each MSP, and add it to the correct tree based on its strand and
* reading frame. Also find the lowest ID value out of all the matches. */
MSP *msp = mspList;
for ( ; msp; msp = msp->next)
{
/* Only add matches/exons to trees. For exons, only add the parent exon;
* it's child UTR/CDSs will be added automatically */
if (mspIsBlastMatch(msp) || msp->type == BLXMSP_EXON || msp->type == BLXMSP_BASIC)
{
/* Find the tree that this MSP should belong to based on its reading frame and strand */
BlxStrand strand = mspGetRefStrand(msp);
const int frame = mspGetRefFrame(msp, bc->seqType);
GtkWidget *tree = detailViewGetTree(detailView, strand, frame);
if (tree)
{
GtkListStore *store = GTK_LIST_STORE(treeGetBaseDataModel(GTK_TREE_VIEW(tree)));
addMspToTree(msp, tree, store);
}
else
{
g_warning("Could not determine alignment list. Sequence may not be shown. (sequence = '%s', q range [%d-%d], s range [%d-%d])\n", mspGetSName(msp), msp->qRange.min(), msp->qRange.max(), msp->sRange.min(), msp->sRange.max());
}
}
}
/* Finally, create a custom-filtered version of the data store for each tree. We do
* this AFTER adding the data so that it doesn't try to re-filter every time we add a row.
* To do: Note that if we're calling this function a second time to add additional rows, the filter
* will already exist so this may be slow and we may need to look into improving this. */
callFuncOnAllDetailViewTrees(detailView, treeCreateFilteredDataModel, NULL);
/* Also create a second data store that will store one sequence per row (as opposed to one
* MSP per row). This data store will be switched in when the user selects 'squash matches'. */
callFuncOnAllDetailViewTrees(detailView, addSequencesToTree, seqList);
}
static gboolean onMouseMoveSnpSplitter(GtkWidget *paned, GdkEventMotion *event, gpointer data)
{
GtkWidget *detailView = GTK_WIDGET(data);
if ((event->state & GDK_BUTTON1_MASK)) /* left button */
{
/* If the splitter position is zero then disable the snp track display */
if (GTK_IS_PANED(paned))
{
const int splitterPos = gtk_paned_get_position(GTK_PANED(paned));
BlxContext *bc = detailViewGetContext(detailView);
if (splitterPos != 0 &&
!(bc->flags[BLXFLAG_SHOW_VARIATION_TRACK] && bc->flags[BLXFLAG_HIGHLIGHT_VARIATIONS]) )
{
/* Show the track. We must also enable highlight-variations or it will not make any
* sense (and probably won't work anyway because the code checks both values). */
bc->flags[BLXFLAG_SHOW_VARIATION_TRACK] = TRUE;
bc->flags[BLXFLAG_HIGHLIGHT_VARIATIONS] = TRUE;
detailViewRefreshAllHeaders(detailView);
DEBUG_OUT("Enabled variations track (exposed by splitter)\n");
}
else if (splitterPos == 0 && bc->flags[BLXFLAG_SHOW_VARIATION_TRACK])
{
/* Disable the variations track. Leave the highlight-varations setting as it is. */
bc->flags[BLXFLAG_SHOW_VARIATION_TRACK] = FALSE;
detailViewRefreshAllHeaders(detailView);
DEBUG_OUT("Disabled variations track (hidden by splitter)\n");
}
}
}
return FALSE; /* allow default handler to continue */
}
/* Utility to create the paned window for the snp track. The otherWidget is the container for all
* the other widgets to go into the 2nd pane of the new paned window. */
GtkWidget* snpTrackCreatePanedWin(GtkWidget *detailView, GtkWidget *snpTrack, GtkWidget *otherWidget)
{
GtkPaned *paned = GTK_PANED(gtk_vpaned_new());
gtk_widget_set_name(GTK_WIDGET(paned), SNP_TRACK_CONTAINER_NAME);
/* Top pane is the snp track. Put it inside a scrollwin */
GtkWidget *snpScrollWin = gtk_scrolled_window_new(NULL, NULL);
gtk_container_add(GTK_CONTAINER(snpScrollWin), snpTrack);
gtk_widget_set_name(GTK_WIDGET(snpScrollWin), SNP_TRACK_SCROLL_WIN_NAME);
gtk_scrolled_window_set_policy(GTK_SCROLLED_WINDOW(snpScrollWin), GTK_POLICY_NEVER, GTK_POLICY_ALWAYS);
gtk_paned_pack1(paned, snpScrollWin, FALSE, TRUE);
gtk_paned_pack2(paned, otherWidget, TRUE, TRUE);
/* Connect to signals for the splitter bar handle */
g_signal_connect(G_OBJECT(paned), "motion-notify-event", G_CALLBACK(onMouseMoveSnpSplitter), detailView);
/* Create properties struct for the paned window, which will cache the splitter position */
panedWindowCreateProperties(GTK_WIDGET(paned), -1);
return GTK_WIDGET(paned);
}
GtkWidget* createDetailView(GtkWidget *blxWindow,
BlxContext *bc,
GtkContainer *parent,
GtkWidget *toolbar,
GtkAdjustment *adjustment,
GtkWidget *fwdStrandGrid,
GtkWidget *revStrandGrid,
MSP *mspList,
GList *columnList,
BlxBlastMode mode,
BlxSeqType seqType,
int numFrames,
const char* const refSeqName,
const int startCoord,
const gboolean sortInverted,
const BlxColumnId sortColumn,
const gboolean optionalDataLoaded,
char *windowColor)
{
/* We'll group the trees in their own container so that we can pass them all around
* together (so that operations like zooming and scrolling can act on the group). The
* trees might be a direct child of this or a grandchild/grand-grandchild, so we will need
* to look at all children recursively and check if they're the correct type. (We'll give
* all of our detail-view trees the same name so that we can identify them.) */
GtkWidget *detailView = gtk_vbox_new(FALSE, 0);
gtk_container_add(parent, detailView);
GtkWidget *panedWin = gtk_vpaned_new();
gtk_widget_set_name(panedWin, DETAIL_VIEW_WIDGET_NAME);
/* Update the sequence column with a custom header type. (Must be done
* before calling createDetailViewHeader) */
createSeqColHeader(detailView, seqType, numFrames, columnList);
/* Create the toolbar. We need to remember the feedback box and status bar so we can set them in the properties. */
GtkWidget *feedbackBox = NULL;
GtkWidget *statusBar = NULL;
GtkWidget *buttonBar = createDetailViewButtonBar(detailView, toolbar, mode, sortColumn, columnList, windowColor, &feedbackBox, &statusBar);
/* Create the header bar. If viewing protein matches include one SNP track in the detail
* view header; otherwise create SNP tracks in each tree header. */
const gboolean singleSnpTrack = (seqType == BLXSEQ_PEPTIDE);
GtkWidget *snpTrack = NULL;
GtkWidget *header = createDetailViewHeader(detailView, seqType, numFrames, columnList, singleSnpTrack, &snpTrack);
/* Create a custom cell renderer to render the sequences in the detail view */
GtkCellRenderer *renderer = sequence_cell_renderer_new();
/* Create the trees. */
GList *fwdStrandTrees = NULL, *revStrandTrees = NULL;
createDetailViewPanes(detailView,
GTK_PANED(panedWin),
renderer,
fwdStrandGrid,
revStrandGrid,
numFrames,
&fwdStrandTrees,
&revStrandTrees,
seqType,
columnList,
refSeqName,
!singleSnpTrack);
/* Add the elements into the main widget */
gtk_box_pack_start(GTK_BOX(detailView), buttonBar, FALSE, TRUE, 0);
if (snpTrack)
{
/* Add the snp track in a pane so it can be resized vs everything below it.
* Top pane is the snp track, bottom pane is everything else (in a vbox) */
GtkWidget *vbox = gtk_vbox_new(FALSE, 0);
gtk_box_pack_start(GTK_BOX(vbox), header, FALSE, TRUE, 0);
gtk_box_pack_start(GTK_BOX(vbox), panedWin, TRUE, TRUE, 0);
GtkWidget *paned = snpTrackCreatePanedWin(detailView, snpTrack, vbox);
gtk_box_pack_start(GTK_BOX(detailView), GTK_WIDGET(paned), TRUE, TRUE, 0);
}
else
{
gtk_box_pack_start(GTK_BOX(detailView), header, FALSE, TRUE, 0);
gtk_box_pack_start(GTK_BOX(detailView), panedWin, TRUE, TRUE, 0);
}
/* Add a coverage view */
CoverageViewProperties *coverageViewP = createCoverageView(blxWindow, bc);
gtk_box_pack_start(GTK_BOX(detailView), coverageViewP->widget(), FALSE, TRUE, 0);
/* Connect signals */
gtk_widget_add_events(detailView, GDK_BUTTON_PRESS_MASK);
g_signal_connect(G_OBJECT(detailView), "button-press-event", G_CALLBACK(onButtonPressDetailView), NULL);
g_signal_connect(G_OBJECT(detailView), "button-release-event", G_CALLBACK(onButtonReleaseDetailView), NULL);
g_signal_connect(G_OBJECT(detailView), "motion-notify-event", G_CALLBACK(onMouseMoveDetailView), NULL);
g_signal_connect(G_OBJECT(detailView), "scroll-event", G_CALLBACK(onScrollDetailView), NULL);
DetailViewProperties *properties = detailViewCreateProperties(detailView,
blxWindow,
bc,
coverageViewP,
renderer,
fwdStrandTrees,
revStrandTrees,
feedbackBox,
statusBar,
columnList,
adjustment,
startCoord,
sortColumn);
coverageViewP->setPanel(properties);
return detailView;
}
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