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/* File: sequencecellrenderer.c
* Author: Gemma Barson, 2009-10-15
* Copyright (c) 2009 - 2012 Genome Research Ltd
* ---------------------------------------------------------------------------
* SeqTools is free software; you can redistribute it and/or
* modify it under the terms of the GNU General Public License
* as published by the Free Software Foundation; either version 3
* of the License, or (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program; if not, write to the Free Software
* Foundation, Inc., 59 Temple Place - Suite 330, Boston, MA 02111-1307, USA.
* or see the on-line version at http://www.gnu.org/copyleft/gpl.txt
* ---------------------------------------------------------------------------
* This file is part of the SeqTools sequence analysis package,
* written by
* Gemma Barson (Sanger Institute, UK) <gb10@sanger.ac.uk>
*
* based on original code by
* Erik Sonnhammer (SBC, Sweden) <Erik.Sonnhammer@sbc.su.se>
*
* and utilizing code taken from the AceDB and ZMap packages, written by
* Richard Durbin (Sanger Institute, UK) <rd@sanger.ac.uk>
* Jean Thierry-Mieg (CRBM du CNRS, France) <mieg@kaa.crbm.cnrs-mop.fr>
* Ed Griffiths (Sanger Institute, UK) <edgrif@sanger.ac.uk>
* Roy Storey (Sanger Institute, UK) <rds@sanger.ac.uk>
* Malcolm Hinsley (Sanger Institute, UK) <mh17@sanger.ac.uk>
*
* Description: See sequencecellrenderer.h
*----------------------------------------------------------------------------
*/
#include <blixemApp/sequencecellrenderer.hpp>
#include <blixemApp/blxcontext.hpp>
#include <blixemApp/detailview.hpp>
#include <blixemApp/detailviewtree.hpp>
#include <blixemApp/blxwindow.hpp>
#include <seqtoolsUtils/blxmsp.hpp>
#include <seqtoolsUtils/utilities.hpp>
#include <gtk/gtkcellrenderertext.h>
#include <stdlib.h>
#include <ctype.h>
#include <math.h>
#include <string.h>
#define SEQUENCE_CELL_RENDERER_NAME "SequenceCellRenderer"
#define GAP_WIDTH_AS_FRACTION 0.375 /* multiplier used to get the width of the "gap" marker based on a fraction of char width */
#define MIN_GAP_WIDTH 2
typedef struct _RenderData
{
BlxContext *bc;
GdkRectangle *cell_area;
GdkWindow *window;
GtkStateType state;
GdkGC *gc;
const BlxStrand qStrand;
const int qFrame;
const int selectedBaseIdx;
IntRange *selectionRange;
gboolean seqSelected;
GList *selectedSeqs;
const int cellXPadding;
const int cellYPadding;
const gdouble charWidth;
const gdouble charHeight;
const IntRange* const displayRange;
const gboolean highlightDiffs;
GdkDrawable *drawable;
GtkWidget *blxWindow;
GdkColor *exonColor;
GdkColor *exonColorSelected;
GdkColor *cdsColor;
GdkColor *cdsColorSelected;
GdkColor *utrColor;
GdkColor *utrColorSelected;
GdkColor *crosshatchColor;
GdkColor *crosshatchColorSelected;
GdkColor *insertionColor;
GdkColor *insertionColorSelected;
GdkColor *matchColor;
GdkColor *matchColorSelected;
GdkColor *consColor;
GdkColor *consColorSelected;
GdkColor *mismatchColor;
GdkColor *mismatchColorSelected;
GdkColor *unalignedSeqColor;
GdkColor *unalignedSeqColorSelected;
GdkColor *exonBoundaryColorStart;
GdkColor *exonBoundaryColorEnd;
GdkColor *polyAColor;
GdkColor *polyAColorSelected;
GdkColor *clipMarkerColor;
int exonBoundaryWidth;
GdkLineStyle exonBoundaryStyle;
GdkLineStyle exonBoundaryStylePartial;
gboolean limitUnalignedBases;
int numUnalignedBases;
} RenderData;
/* Properties */
enum
{
PROP_0,
PROP_TEXT,
PROP_MSP,
PROP_DATA
};
/* Some boring function declarations: GObject type system stuff */
static void sequence_cell_renderer_init (SequenceCellRenderer *cellrenderersequence);
static void sequence_cell_renderer_class_init (SequenceCellRendererClass *klass);
static void sequence_cell_renderer_finalize (GObject *gobject);
static gboolean mspGetVisibleRange(MSP *msp, RenderData *data, IntRange *result);
void drawAllVisibleExonBoundaries(GtkWidget *tree, RenderData *data);
static void mspDrawSequenceText(GtkWidget *tree,
gchar *displayText,
const IntRange* const segmentRange,
RenderData *data);
static void segmentGetCoordsForBaseIdx(const int segmentIdx,
const IntRange* const segmentRange,
RenderData *data,
int *x,
int* y);
/* These functions are the heart of our custom cell renderer: */
static void sequence_cell_renderer_get_size (GtkCellRenderer *cell,
GtkWidget *widget,
GdkRectangle *cell_area,
gint *x_offset,
gint *y_offset,
gint *width,
gint *height);
static void sequence_cell_renderer_render (GtkCellRenderer *cell,
GdkDrawable *window,
GtkWidget *tree,
GdkRectangle *background_area,
GdkRectangle *cell_area,
GdkRectangle *expose_area,
GtkCellRendererState flags);
static void sequence_cell_renderer_get_property (GObject *object,
guint param_id,
GValue *value,
GParamSpec *pspec);
static void sequence_cell_renderer_set_property (GObject *object,
guint param_id,
const GValue *value,
GParamSpec *pspec);
static gpointer parent_class;
/***************************************************************************/
/* Custom version of gtk_paint_layout that draws the same thing to two GdkDrawables */
static void paintLayout2(GtkStyle *style,
GdkDrawable *drawable1,
GdkDrawable *drawable2,
GtkStateType state_type,
gboolean use_text,
GdkRectangle *area,
GtkWidget *widget,
gchar *detail,
gint x,
gint y,
PangoLayout *layout)
{
if (drawable1)
gtk_paint_layout (style, drawable1, state_type, use_text, area, widget, detail, x, y, layout);
if (drawable2)
gtk_paint_layout (style, drawable2, state_type, use_text, area, widget, detail, x, y, layout);
}
/* Custom version of gdk_draw_line that draws the same thing to two GdkDrawables */
void drawLine2(GdkDrawable *drawable1,
GdkDrawable *drawable2,
GdkGC *gc,
gint x1,
gint y1,
gint x2,
gint y2)
{
if (drawable1)
{
// cairo_t *cr = gdk_cairo_create(drawable1);
// cairo_set_line_width(cr, 0.5);
// cairo_move_to(cr, x1 + 0.5, y1);
// cairo_line_to(cr, x2 + 0.5, y2);
// cairo_stroke(cr);
// cairo_destroy(cr);
gdk_draw_line(drawable1, gc, x1, y1, x2, y2);
}
if (drawable2)
{
// cairo_t *cr = gdk_cairo_create(drawable2);
// cairo_set_line_width(cr, 0.5);
// cairo_move_to(cr, x1 + 0.5, y1);
// cairo_line_to(cr, x2 + 0.5, y2);
// cairo_stroke(cr);
// cairo_destroy(cr);
gdk_draw_line(drawable2, gc, x1, y1, x2, y2);
}
}
/* Custom version of gdk_draw_rectangle that draws the same thing to two GdkDrawables */
void drawRectangle2(GdkDrawable *drawable1,
GdkDrawable *drawable2,
GdkGC *gc,
gboolean filled,
gint x,
gint y,
gint width,
gint height)
{
// cairo_t *cr = gdk_cairo_create(drawable1);
// cairo_rectangle(cr, x, y, width, height);
// cairo_fill(cr);
// cairo_destroy(cr);
if (drawable1)
{
gdk_draw_rectangle(drawable1, gc, filled, x, y, width, height);
}
if (drawable2)
{
gdk_draw_rectangle(drawable2, gc, filled, x, y, width, height);
}
}
/* Fill a rectangle with crosshatch lines */
void drawCrosshatchRectangle(GdkDrawable *drawable1,
GdkDrawable *drawable2,
GdkGC *gc,
gint x,
gint y,
gint width,
gint height)
{
/* Draw diagonal lines from top-right to bottom-left. */
drawLine2(drawable1, drawable2, gc, x + width, y, x, y + height);
drawLine2(drawable1, drawable2, gc, x + width / 2, y, x, y + height / 2);
drawLine2(drawable1, drawable2, gc, x + width, y + height / 2, x + width / 2, y + height);
}
/***************************************************************************
*
* sequence_cell_renderer_get_type: here we register our type with
* the GObject type system if we
* haven't done so yet. Everything
* else is done in the callbacks.
*
***************************************************************************/
GType
sequence_cell_renderer_get_type (void)
{
static GType cell_sequence_type = 0;
if (cell_sequence_type == 0)
{
static const GTypeInfo cell_sequence_info =
{
sizeof (SequenceCellRendererClass),
NULL, /* base_init */
NULL, /* base_finalize */
(GClassInitFunc) sequence_cell_renderer_class_init,
NULL, /* class_finalize */
NULL, /* class_data */
sizeof (SequenceCellRenderer),
0, /* n_preallocs */
(GInstanceInitFunc) sequence_cell_renderer_init,
};
/* Derive from GtkCellRenderer */
cell_sequence_type = g_type_register_static (GTK_TYPE_CELL_RENDERER,
"SequenceCellRenderer",
&cell_sequence_info,
(GTypeFlags)0);
}
return cell_sequence_type;
}
/***************************************************************************
*
* sequence_cell_renderer_init: set some default properties of the
* parent (GtkCellRendererText).
*
***************************************************************************/
static void
sequence_cell_renderer_init (SequenceCellRenderer *cellrenderersequence)
{
GTK_CELL_RENDERER(cellrenderersequence)->mode = GTK_CELL_RENDERER_MODE_INERT;
GTK_CELL_RENDERER(cellrenderersequence)->xalign = 0;
GTK_CELL_RENDERER(cellrenderersequence)->yalign = 0;
GTK_CELL_RENDERER(cellrenderersequence)->xpad = 0;
GTK_CELL_RENDERER(cellrenderersequence)->ypad = 0;
cellrenderersequence->data = NULL;
cellrenderersequence->mspGList = NULL;
cellrenderersequence->text = NULL;
}
/***************************************************************************
*
* sequence_cell_renderer_class_init:
*
* If you want cells that can be activated on their own (ie. not
* just the whole row selected) or cells that are editable, you
* will need to override 'activate' and 'start_editing'.
*
***************************************************************************/
static void
sequence_cell_renderer_class_init (SequenceCellRendererClass *klass)
{
GtkCellRendererClass *cell_class = GTK_CELL_RENDERER_CLASS(klass);
GObjectClass *object_class = G_OBJECT_CLASS(klass);
parent_class = g_type_class_peek_parent (klass);
object_class->finalize = sequence_cell_renderer_finalize;
/* Override the two crucial functions that are the heart
* of a cell renderer in the parent class */
cell_class->get_size = sequence_cell_renderer_get_size;
cell_class->render = sequence_cell_renderer_render;
/* Override functions to get/set properties */
object_class->get_property = sequence_cell_renderer_get_property;
object_class->set_property = sequence_cell_renderer_set_property;
g_object_class_install_property (object_class,
PROP_DATA,
g_param_spec_pointer (RENDERER_DATA_PROPERTY,
"Data",
"Pointer to the msp",
G_PARAM_WRITABLE));
g_object_class_install_property (object_class,
PROP_MSP,
g_param_spec_pointer (RENDERER_SEQUENCE_PROPERTY,
"Sequence",
"Pointer to an msp whose sequence to display",
G_PARAM_WRITABLE));
g_object_class_install_property (object_class,
PROP_TEXT,
g_param_spec_string (RENDERER_TEXT_PROPERTY,
"Text",
"Text field",
NULL,
G_PARAM_WRITABLE));
}
/***************************************************************************
*
* sequence_cell_renderer_finalize: free any resources here
*
***************************************************************************/
static void
sequence_cell_renderer_finalize (GObject *object)
{
/*
SequenceCellRenderer *cellrenderersequence = SEQUENCE_CELL_RENDERER(object);
*/
/* Free any dynamically allocated resources here */
(* G_OBJECT_CLASS (parent_class)->finalize) (object);
}
/***************************************************************************
*
* sequence_cell_renderer_new: return a new cell renderer instance
*
***************************************************************************/
GtkCellRenderer *
sequence_cell_renderer_new (void)
{
return (GtkCellRenderer*)g_object_new(SEQUENCE_CELL_RENDERER_TYPE, NULL);
}
/***************************************************************************
*
* sequence_cell_renderer_set_property:
*
***************************************************************************/
static void setAllPropertiesNull(SequenceCellRenderer *renderer)
{
if (renderer->text)
g_free(renderer->text);
renderer->text = NULL;
renderer->mspGList = NULL;
}
static void
sequence_cell_renderer_set_property (GObject *object,
guint param_id,
const GValue *value,
GParamSpec *pspec)
{
SequenceCellRenderer *renderer = SEQUENCE_CELL_RENDERER(object);
switch (param_id)
{
case PROP_DATA:
/* Additional data. DON'T reset other properties. */
renderer->data = (GList*)g_value_get_pointer(value);
break;
case PROP_MSP:
/* We set either the msp data or the text data, not both;
* so make sure nothing else is set */
setAllPropertiesNull(renderer);
renderer->mspGList = (GList*)g_value_get_pointer(value);
break;
case PROP_TEXT:
/* We set either the msp data or the text data, not both;
* so make sure nothing else is set */
setAllPropertiesNull(renderer);
renderer->text = g_strdup(g_value_get_string(value));
break;
default:
G_OBJECT_WARN_INVALID_PROPERTY_ID (object, param_id, pspec);
break;
};
}
/***************************************************************************
*
* sequence_cell_renderer_get_property:
*
***************************************************************************/
static void
sequence_cell_renderer_get_property (GObject *object,
guint param_id,
GValue *value,
GParamSpec *pspec)
{
SequenceCellRenderer *renderer = SEQUENCE_CELL_RENDERER(object);
switch (param_id)
{
case PROP_DATA:
g_value_set_pointer(value, renderer->data);
break;
case PROP_MSP:
g_value_set_pointer(value, renderer->mspGList);
break;
case PROP_TEXT:
g_value_set_string(value, renderer->text);
break;
default:
G_OBJECT_WARN_INVALID_PROPERTY_ID (object, param_id, pspec);
break;
};
}
static void
get_size (GtkCellRenderer *cell,
GtkWidget *widget,
GdkRectangle *cell_area,
gint *x_offset,
gint *y_offset,
gint *width,
gint *height)
{
if (height)
*height = cell->height;
if (width)
*width = cell->width;
if (cell_area)
cell_area->height = cell->height;
}
/* Render function for a cell that contains simple text */
static void rendererDrawSimpleText(SequenceCellRenderer *renderer,
GtkWidget *tree,
GdkWindow *window,
GtkStateType state,
GdkRectangle *cell_area)
{
gchar *displayText = renderer->text;
PangoLayout *layout = gtk_widget_create_pango_layout(tree, displayText);
PangoFontDescription *font_desc = pango_font_description_copy(tree->style->font_desc);
pango_layout_set_font_description(layout, font_desc);
pango_font_description_free(font_desc);
paintLayout2(tree->style,
window,
widgetGetDrawable(tree),
state,
TRUE,
NULL,
tree,
NULL,
cell_area->x - treeGetCellXPadding(tree),
cell_area->y - treeGetCellYPadding(tree),
layout);
g_object_unref(layout);
}
/* The given renderer is an MSP. This function checks if there is a base index
* selected and, if so, colors the background for that base with the given color. */
static void highlightSelectedBase(const int selectedBaseIdx,
const GdkColor *highlightColor,
RenderData *data)
{
if (selectedBaseIdx != UNSET_INT && valueWithinRange(selectedBaseIdx, data->displayRange))
{
/* Convert the display-range index to a 0-based index for the section of sequence displayed */
const int segmentIdx = selectedBaseIdx - data->displayRange->min();
int x, y;
segmentGetCoordsForBaseIdx(segmentIdx, data->displayRange, data, &x, &y);
gdk_gc_set_foreground(data->gc, highlightColor);
drawRectangle2(data->window, data->drawable, data->gc, TRUE, x, y, roundNearest(data->charWidth), roundNearest(data->charHeight));
}
}
/* Returns true if the min coord (or max coord, if 'start' is false) is a
* partial codon, i.e. if it does not start at base 1 (if start) or end at
* base 3 (if end)... or vice versa if the display is reversed */
static gboolean exonCoordIsPartialCodon(const MSP* const msp, const gboolean start, RenderData *data, int *displayIdxOut)
{
/* To be a complete codon, if we're at the start the the coord must be base 1
* or if we're at the end then coord must be base 3 */
const int reqdBase = (start != data->bc->displayRev) ? 1 : data->bc->numFrames;
/* Calculate the actual base number of the start/end coord */
const int dnaIdx = start ? msp->qRange.min() : msp->qRange.max();
int baseNum = UNSET_INT;
const int frame = mspGetRefFrame(msp, data->bc->seqType);
const int displayIdx = convertDnaIdxToDisplayIdx(dnaIdx, data->bc->seqType, frame,
data->bc->numFrames, data->bc->displayRev, &data->bc->refSeqRange, &baseNum);
if (displayIdxOut)
*displayIdxOut = displayIdx;
gboolean isPartial = (baseNum != reqdBase);
return isPartial;
}
static gboolean coordIsSelected(RenderData *data, const int coord)
{
gboolean result = FALSE;
gboolean coordSelected = FALSE;
if (data->selectionRange && valueWithinRange(coord, data->selectionRange))
coordSelected = TRUE;
if (data->selectionRange && data->selectionRange->length() > 1)
{
/* There is a range of selected coords. We highlight the base if it's in this range.
* We only highlight selection-range coords for the currently selected sequence(s), unless
* there are no selected sequences, in which case highlight all rows. */
if (data->seqSelected || !data->selectedSeqs || g_list_length(data->selectedSeqs) < 1)
result = coordSelected;
else
result = FALSE;
}
else
{
/* We highlight the base if the coord is selected OR the sequence is selected but not if
* both are true, i.e. we invert the highlight colour if both the coord and the sequence
* are selected. */
result = (coordSelected != data->seqSelected);
}
return result;
}
/* Highlight the start (min) peptide of the given msp if it is a partial codon, i.e. if it
* does not start at base 1 (or end at base 3, if the display is reversed). OR highlight the end
* (max) codon if 'start' is FALSE. The x/y coords give the top left corner of the peptide. */
static void exonHighlightPartialCodons(const MSP* const msp,
const gboolean start,
const int x,
const int y,
RenderData *data)
{
int displayIdx = UNSET_INT;
gboolean isPartial = exonCoordIsPartialCodon(msp, start, data, &displayIdx);
if (isPartial && valueWithinRange(displayIdx, data->displayRange))
{
/* It's not a complete codon, so highlight this base */
const gboolean isSelected = coordIsSelected(data, displayIdx);
GdkColor *color = (isSelected ? data->crosshatchColorSelected : data->crosshatchColor);
gdk_gc_set_foreground(data->gc, color);
drawCrosshatchRectangle(data->window, data->drawable, data->gc, x, y, data->charWidth, data->charHeight);
}
}
/* The given renderer is an MSP that is drawn as a simple box. This function draws the feature
* or part of the feature that is in view, if it is within the current display range. */
static void drawBoxFeature(SequenceCellRenderer *renderer,
MSP *msp,
GtkWidget *tree,
RenderData *data)
{
if (mspLayerIsVisible(msp))
{
IntRange segmentRange;
if (!mspGetVisibleRange(msp, data, &segmentRange))
{
return;
}
const int segmentLen = segmentRange.length();
int x, y;
segmentGetCoordsForBaseIdx(0, &segmentRange, data, &x, &y);
const int width = ceil((gdouble)segmentLen * data->charWidth);
const int height = roundNearest(data->charHeight) - 1;
/* Just draw one big rectangle the same color for the whole thing. Color depends if row
* is selected, but note we don't highlight the whole row if a coord range is also selected. */
gboolean fill = TRUE;
const gboolean highlightExon = data->seqSelected && (!data->selectionRange || data->selectionRange->length() < 2);
const GdkColor *color = mspGetColor(msp,
data->bc->defaultColors,
BLXCOLOR_BACKGROUND,
msp->sSequence,
highlightExon,
data->bc->usePrintColors,
fill,
BLXCOLOR_EXON_FILL, BLXCOLOR_EXON_LINE,
BLXCOLOR_CDS_FILL, BLXCOLOR_CDS_LINE,
BLXCOLOR_UTR_FILL, BLXCOLOR_UTR_LINE,
FALSE);
gdk_gc_set_foreground(data->gc, color);
drawRectangle2(data->window, data->drawable, data->gc, fill, x, y, width, height);
/* If a base is selected, highlight it. Its color depends on whether it the base is within the exon range or not. */
if (data->selectionRange)
{
int i = 0;
for (i = data->selectionRange->min(); i <= data->selectionRange->max(); ++i)
{
if (i != UNSET_INT && valueWithinRange(i, &segmentRange))
{
/* Call coordIsSelected to determine highlighting of this base */
const gboolean highlightBase = coordIsSelected(data, i);
color = mspGetColor(msp, data->bc->defaultColors, BLXCOLOR_BACKGROUND, msp->sSequence, highlightBase, data->bc->usePrintColors, TRUE, BLXCOLOR_EXON_FILL, BLXCOLOR_EXON_LINE, BLXCOLOR_CDS_FILL, BLXCOLOR_CDS_LINE, BLXCOLOR_UTR_FILL, BLXCOLOR_UTR_LINE);
highlightSelectedBase(i, color, data);
}
}
}
/* And now draw the outline (do this after the base highlight because otherwise that will
* erase the boundary) */
fill = FALSE;
color = mspGetColor(msp, data->bc->defaultColors, BLXCOLOR_BACKGROUND, msp->sSequence, data->seqSelected, data->bc->usePrintColors, fill, BLXCOLOR_EXON_FILL, BLXCOLOR_EXON_LINE, BLXCOLOR_CDS_FILL, BLXCOLOR_CDS_LINE, BLXCOLOR_UTR_FILL, BLXCOLOR_UTR_LINE);
gdk_gc_set_foreground(data->gc, color);
drawRectangle2(data->window, data->drawable, data->gc, fill, x, y, width, height);
/* If the start or end index is not a full codon, highlight it in a different color */
exonHighlightPartialCodons(msp, !data->bc->displayRev, x, y, data);
exonHighlightPartialCodons(msp, data->bc->displayRev, x + width - data->charWidth, y, data);
}
}
/* Get the base from the given sequence at the given index. Converts to
* upper or lower case as appropriate for the sequence type. The given index is
* assumed to be 1-based. If the given BlxSequence has null sequence data, then
* this function returns a padding character instead */
static char blxSeqGetMatchSeqBase(BlxSequence *blxSeq, const int sIdx, const BlxSeqType seqType)
{
char result = SEQUENCE_CHAR_PAD;
const char *sequence = blxSequenceGetSequence(blxSeq);
if (sequence && sIdx <= (int)strlen(sequence))
{
result = sequence[sIdx - 1];
result = convertBaseToCorrectCase(result, seqType);
}
return result;
}
/* Color in the background of a particular base in the given match sequence. Returns
* the calculated index into the match sequence (for effiency, so that we don't have to
* recalculate it later on). Returns the equivalent index in the subject sequence, or
* UNSET_INT if there is none. */
static void mspDrawBaseBg(MSP *msp,
const int segmentIdx,
const IntRange* const segmentRange,
char *refSeqSegment,
RenderData *data,
const int x,
const int y,
gchar *displayText,
int *sIdx,
int *qIdx)
{
char sBase = '\0';
GdkColor *baseBgColor = NULL;
/* From the segment index, find the display index and the ref seq coord */
const int displayIdx = segmentRange->min() + segmentIdx;
*qIdx = convertDisplayIdxToDnaIdx(displayIdx, data->bc->seqType, data->qFrame, 1, data->bc->numFrames, data->bc->displayRev, &data->bc->refSeqRange);
/* Find the match-sequence coord at this ref-seq coord */
gboolean found_sIdx = mspGetMatchCoord(msp, *qIdx, data->seqSelected, data->numUnalignedBases, data->bc, sIdx);
/* Highlight the base if its base index is selected, or if its sequence is selected.
* (If it is selected in both, show it in the normal color) */
gboolean selected = coordIsSelected(data, displayIdx);
if (!valueWithinRange(*qIdx, &msp->qRange))
{
/* We're outside the alignment range. There might still be a base to display if
* we're displaying unaligned parts of the match sequence or polyA tails; otherwise, we
* show nothing. */
if (found_sIdx)
{
sBase = blxSeqGetMatchSeqBase(msp->sSequence, *sIdx, data->bc->seqType);
if (data->bc->flags[BLXFLAG_SHOW_POLYA_SITE] &&
(!data->bc->flags[BLXFLAG_SHOW_POLYA_SITE_SELECTED] || data->seqSelected) &&
mspCoordInPolyATail(*qIdx, msp))
{
baseBgColor = selected ? data->polyAColorSelected : data->polyAColor;
}
else
{
baseBgColor = selected ? data->unalignedSeqColorSelected : data->unalignedSeqColor;
}
}
}
else if (!found_sIdx)
{
/* We're inside the alignment range but there is no base to display: we must be in a deletion. */
sBase = SEQUENCE_CHAR_DELETION;
baseBgColor = selected ? data->mismatchColorSelected : data->mismatchColor;
}
else
{
/* There is a base in the match sequence. See if it matches the ref sequence */
sBase = blxSeqGetMatchSeqBase(msp->sSequence, *sIdx, data->bc->seqType);
char qBase = refSeqSegment[segmentIdx];
if (tolower(sBase) == tolower(qBase))
{
/* Match */
baseBgColor = selected ? data->matchColorSelected : data->matchColor;
/* If we're highlighting differences, don't show this base (show a dash instead) */
if (data->highlightDiffs)
{
sBase = SEQUENCE_CHAR_BLANK;
}
}
else if (data->bc->blastMode != BLXMODE_BLASTN && PAM120[aa_atob[(unsigned int)qBase]-1 ][aa_atob[(unsigned int)sBase]-1 ] > 0)
{
/* 'Conserved' match (i.e. similar amino acid) */
baseBgColor = selected ? data->consColorSelected : data->consColor;
}
else
{
/* Mismatch */
baseBgColor = selected ? data->mismatchColorSelected : data->mismatchColor;
}
}
/* Draw the background color */
if (baseBgColor)
{
gdk_gc_set_foreground(data->gc, baseBgColor);
drawRectangle2(data->window, data->drawable, data->gc, TRUE, x, y, ceil(data->charWidth), roundNearest(data->charHeight));
}
if (sBase != '\0')
{
/* Add this character into the display text */
displayText[segmentIdx] = sBase;
}
else
{
displayText[segmentIdx] = ' ';
}
}
static PangoLayout* pangoGetLayoutFromText(gchar *displayText, GtkWidget *tree, PangoFontDescription *font_desc)
{
PangoLayout *layout = gtk_widget_create_pango_layout(tree, displayText);
pango_layout_set_font_description(layout, font_desc);
return layout;
}
/* Return the x/y coords for the top-left corner where we want to draw the base
* with the given index in the segment, where the segment starts at the given
* index in the display. */
static void segmentGetCoordsForBaseIdx(const int segmentIdx,
const IntRange* const segmentRange,
RenderData *data,
int *x,
int* y)
{
/* Find the start of the segment with respect to the display range */
const int startPos = segmentRange->min() - data->displayRange->min();
/* Find the position of the character within the segment */
int charIdx = startPos + segmentIdx;
/* Calculate the coords */
*x = data->cell_area->x - data->cellXPadding + (int)((gdouble)charIdx * data->charWidth);
*y = data->cell_area->y - data->cellYPadding;
}
/* Draw the start/end boundaries for the given MSP if it is an exon whose start/end
* coords are within the current display range */
static gboolean exonDrawBoundary(const MSP *msp, RenderData *rd)
{
if (msp && msp->type == BLXMSP_EXON)
{
/* Get the msp's start/end in terms of the display coords */
const IntRange* const mspRange = mspGetDisplayRange(msp);
if (valueWithinRange(mspRange->min(), rd->displayRange))
{
/* Draw the lower index. The color and line style depend on whether it's the start or end index. */
GdkColor *color = rd->bc->displayRev ? rd->exonBoundaryColorEnd : rd->exonBoundaryColorStart;
gdk_gc_set_foreground(rd->gc, color);
/* Check if it's the boundary of a partial codon - we'll draw a dotted
* line if it is, to indicate that the boundary is not exactly at this position. */
const gboolean isPartial = exonCoordIsPartialCodon(msp, !rd->bc->displayRev, rd, NULL);
GdkLineStyle lineStyle = isPartial ? rd->exonBoundaryStylePartial : rd->exonBoundaryStyle;
gdk_gc_set_line_attributes(rd->gc, rd->exonBoundaryWidth, lineStyle, GDK_CAP_BUTT, GDK_JOIN_MITER);
const int idx = mspRange->min() - rd->displayRange->min();
int x = UNSET_INT, y = UNSET_INT;
segmentGetCoordsForBaseIdx(idx, rd->displayRange, rd, &x, &y);
drawLine2(rd->window, rd->drawable, rd->gc, x, y, x, y + roundNearest(rd->charHeight));
}
if (valueWithinRange(mspRange->max(), rd->displayRange))
{
/* Draw the upper index. The color and line style depend on whether it's the start or end index. */
GdkColor *color = rd->bc->displayRev ? rd->exonBoundaryColorStart : rd->exonBoundaryColorEnd;
gdk_gc_set_foreground(rd->gc, color);
/* Check if it's the boundary of a partial codon - we'll draw a dotted
* line if it is, to indicate that the boundary is not exactly at this position. */
const gboolean isPartial = exonCoordIsPartialCodon(msp, rd->bc->displayRev, rd, NULL);
GdkLineStyle lineStyle = isPartial ? rd->exonBoundaryStylePartial : rd->exonBoundaryStyle;
gdk_gc_set_line_attributes(rd->gc, rd->exonBoundaryWidth, lineStyle, GDK_CAP_BUTT, GDK_JOIN_MITER);
const int idx = mspRange->max() + 1 - rd->displayRange->min();
int x = UNSET_INT, y = UNSET_INT;
segmentGetCoordsForBaseIdx(idx, rd->displayRange, rd, &x, &y);
drawLine2(rd->window, rd->drawable, rd->gc, x, y, x, y + roundNearest(rd->charHeight));
}
}
return FALSE;
}
/* Draw the boundaries of all exons in the given tree that are within the current
* display range */
void drawAllVisibleExonBoundaries(GtkWidget *tree, RenderData *data)
{
/* Loop through all MSPs. */
const MSP *msp = blxWindowGetMspList(data->blxWindow);
for ( ; msp; msp = msp->next)
{
if (mspIsExon(msp) && mspGetRefFrame(msp, data->bc->seqType) == data->qFrame && mspGetRefStrand(msp) == data->qStrand)
{
exonDrawBoundary(msp, data);
}
}
}
/* Draw a vertical yellow line to indicate an insertion between two bases in the match sequence */
static void mspDrawInsertionMarker(int sIdx,
int lastFoundSIdx,
int qIdx,
int lastFoundQIdx,
int x,
int y,
RenderData *data)
{
if (sIdx != UNSET_INT && lastFoundSIdx != UNSET_INT && abs(sIdx - lastFoundSIdx) > 1)
{
/* There is a gap between this index and the previous one (in a left-to-right sense),
* so draw an insertion marker between these two bases (at the position given by x and y). */
/* This is not very sophisticated - just uses a fudge factor to find a suitable width and
* draws it half over the current base and half over the previous one. */
int gapWidth = roundNearest(data->charWidth * GAP_WIDTH_AS_FRACTION);
if (gapWidth < MIN_GAP_WIDTH)
{
gapWidth = MIN_GAP_WIDTH;
}
/* No selections to worry about. Draw the whole thing in the normal color */
gdk_gc_set_foreground(data->gc, data->insertionColor);
drawRectangle2(data->window, data->drawable, data->gc, TRUE, x - gapWidth/2, y, gapWidth, roundNearest(data->charHeight));
}
}
/* Draw the given sequence text at the given coords */
static void mspDrawSequenceText(GtkWidget *tree,
gchar *displayText,
const IntRange* const segmentRange,
RenderData *data)
{
if (g_utf8_validate(displayText, -1, NULL))
{
/* Get the coords for the first base. The display text should have been
* was constructed such that everything else will line up from here. */
int x, y;
segmentGetCoordsForBaseIdx(0, segmentRange, data, &x, &y);
PangoFontDescription *font_desc = pango_font_description_copy(tree->style->font_desc);
PangoLayout *layout = pangoGetLayoutFromText(displayText, tree, font_desc);
pango_font_description_free(font_desc);
if (layout)
{
paintLayout2(tree->style, data->window, data->drawable, data->state, TRUE, NULL, tree, NULL, x, y, layout);
g_object_unref(layout);
}
else
{
g_warning("Error creating layout while trying to display sequence:\n%s\n", displayText);
}
}
else
{
g_warning("Invalid string constructed when trying to display sequence.\n");
}
}
/* Get the range of the msp that is inside the currently displayed range. The
* return value is FALSE if no part of the msp is visible. The result coords
* are in display coords. */
static gboolean mspGetVisibleRange(MSP *msp, RenderData *data, IntRange *result)
{
gboolean found = FALSE;
/* Get the full display range of the MSP (including any portions of unaligned sequence etc.) */
const IntRange *fullRange = mspGetFullDisplayRange(msp, data->seqSelected, data->bc);
result->set(fullRange);
if (rangesOverlap(result, data->displayRange))
{
/* Limit the returned range to the display range. */
result->boundsLimit(data->displayRange, FALSE);
found = TRUE;
}
else
{
/* No portion of the MSP range is in the display range, so return unset range */
result->reset();
}
return found;
}
/* If the current MSP has been clipped (i.e. extends outside the current reference
* sequence range) then draw a marker at the clip point so that the user knows there
* is more data for the match that isn't shown. */
static void mspDrawClippedMarker(const MSP* const msp,
const int qIdx,
const int segmentIdx,
const IntRange* const segmentRange,
const int x,
const int y,
RenderData *data)
{
gboolean clipStart = FALSE;
gboolean clipEnd = FALSE;
/* Check if we're at the very first/last index in the reference sequence range */
if (qIdx == data->bc->refSeqRange.min() && msp->qRange.min() < qIdx)
{
if (data->bc->displayRev)
{
clipEnd = TRUE;
}
else
{
clipStart = TRUE;
}
}
else if (qIdx == data->bc->refSeqRange.max() && msp->qRange.max() > qIdx)
{
if (data->bc->displayRev)
{
clipStart = TRUE;
}
else
{
clipEnd = TRUE;
}
}
if (clipStart || clipEnd)
{
gdouble gapWidth = data->charWidth * GAP_WIDTH_AS_FRACTION;
const int xPos = clipStart ? x : roundNearest((gdouble)x + data->charWidth - gapWidth);
gdk_gc_set_foreground(data->gc, data->clipMarkerColor);
drawRectangle2(data->window, data->drawable, data->gc, TRUE, xPos, y, gapWidth, roundNearest(data->charHeight));
}
}
/* Draw the the given MSP. As well as the sequence text this also draws the background
* color for each base based on how well it matches the reference sequence, and also
* draws insertion markers and "clipped" markers.
* Optionally returns the visible range of the MSP in segmentRange_out. */
static void drawMsp(SequenceCellRenderer *renderer,
MSP *msp,
GtkWidget *tree,
RenderData *data)
{
/* Extract the section of the reference sequence that we're interested in. */
IntRange segmentRange;
if (!mspGetVisibleRange(msp, data, &segmentRange))
{
return;
}
/* The ref seq is in nucleotide coords, so convert the segment coords to nucleotide coords */
const int coord1 = convertDisplayIdxToDnaIdx(segmentRange.min(), data->bc->seqType, data->qFrame, 1, data->bc->numFrames, data->bc->displayRev, &data->bc->refSeqRange);
const int coord2 = convertDisplayIdxToDnaIdx(segmentRange.max(), data->bc->seqType, data->qFrame, data->bc->numFrames, data->bc->numFrames, data->bc->displayRev, &data->bc->refSeqRange);
IntRange qRange(coord1, coord2);
GError *error = NULL;
gchar *refSeqSegment = getSequenceSegment(data->bc->refSeq,
&qRange,
data->qStrand,
BLXSEQ_DNA, /* ref seq is always in nucleotide coords */
data->bc->seqType, /* required segment is in display coords */
data->qFrame,
data->bc->numFrames,
&data->bc->refSeqRange,
data->bc->blastMode,
data->bc->geneticCode,
data->bc->displayRev,
data->bc->displayRev,
TRUE,
&error);
if (!refSeqSegment)
{
g_assert(error);
prefixError(error, "Could not draw alignment for sequence '%s'. ", mspGetSName(msp));
reportAndClearIfError(&error, G_LOG_LEVEL_WARNING);
return;
}
else
{
/* If there's an error but the sequence was still returned it's a non-critical warning */
reportAndClearIfError(&error, G_LOG_LEVEL_WARNING);
}
/* We'll populate a string with the characters we want to display as we loop through the indices. */
const int segmentLen = strlen(refSeqSegment);
gchar displayText[segmentLen + 1];
displayText[0] = '\0';
int lastFoundSIdx = UNSET_INT; /* remember the last index where we found a valid base */
int lastFoundQIdx = UNSET_INT; /* remember the last index where we found a valid base */
int segmentIdx = 0;
for ( ; segmentIdx < segmentLen; ++segmentIdx)
{
int x = UNSET_INT, y = UNSET_INT;
segmentGetCoordsForBaseIdx(segmentIdx, &segmentRange, data, &x, &y);
/* Find the base in the match sequence and draw the background color according to how well it matches */
int sIdx = UNSET_INT, qIdx = UNSET_INT;
mspDrawBaseBg(msp, segmentIdx, &segmentRange, refSeqSegment, data, x, y, displayText, &sIdx, &qIdx);
/* If there is an insertion (i.e. extra bases on the match sequence) between this
* and the previous coord, draw a marker */
mspDrawInsertionMarker(sIdx, lastFoundSIdx, qIdx, lastFoundQIdx, x, y, data);
/* If this match has been clipped, draw a marker to indicate as such */
mspDrawClippedMarker(msp, qIdx, segmentIdx, &segmentRange, x, y, data);
if (sIdx != UNSET_INT)
{
lastFoundSIdx = sIdx;
lastFoundQIdx = qIdx;
}
}
/* Null-terminate the string */
displayText[segmentLen] = '\0';
// insertChar(displayText, &segmentIdx, '\0', msp);
/* Draw the sequence text */
mspDrawSequenceText(tree, displayText, &segmentRange, data);
g_free(refSeqSegment);
}
/* Draw a colinearity line between the two given MSPs, if applicable */
static void mspDrawColinearityLine(const MSP* msp1, const MSP* msp2, const gboolean selected, RenderData *data)
{
if (msp1 && msp2 && data && data->bc)
{
/* If the display is reversed we need to swap the order of the msps */
if (data->bc->displayRev)
{
const MSP* tmp = msp1;
msp1 = msp2;
msp2 = tmp;
}
ColinearityType colinearityType = COLINEAR_INVALID;
if ((data->bc->displayRev && msp1->qStrand == BLXSTRAND_FORWARD) || (!data->bc->displayRev && msp1->qStrand != BLXSTRAND_FORWARD))
colinearityType = mspIsColinear(msp2, msp1);
else
colinearityType = mspIsColinear(msp1, msp2);
if (colinearityType != COLINEAR_INVALID)
{
/* get the line color */
GdkColor *color = NULL;
if (colinearityType == COLINEAR_PERFECT)
color = getGdkColor(BLXCOLOR_COLINEAR_PERFECT, data->bc->defaultColors, FALSE, data->bc->usePrintColors);
else if (colinearityType == COLINEAR_IMPERFECT)
color = getGdkColor(BLXCOLOR_COLINEAR_IMPERFECT, data->bc->defaultColors, FALSE, data->bc->usePrintColors);
else
color = getGdkColor(BLXCOLOR_COLINEAR_NOT, data->bc->defaultColors, FALSE, data->bc->usePrintColors);
gdk_gc_set_foreground(data->gc, color);
/* Get the coords of the line */
const int y = data->cell_area->y + (data->charHeight / 2);
int x1 = data->cell_area->x + ((msp1->displayRange.max(true, data->bc->displayRev) - data->displayRange->min()) * data->charWidth); /* +1 to get rightmost edge of char */
int x2 = data->cell_area->x + ((msp2->displayRange.min(true, data->bc->displayRev) - data->displayRange->min()) * data->charWidth) - 1; /* -1 offset by 1 pixel so we don't overdraw the char */
if (x1 < data->cell_area->x + data->cell_area->width && x2 > data->cell_area->x)
{
if (x1 < data->cell_area->x)
x1 = data->cell_area->x;
if (x2 > data->cell_area->x + data->cell_area->width)
x2 = data->cell_area->x + data->cell_area->width;
drawLine2(data->window, data->drawable, data->gc, x1, y, x2, y);
}
}
}
}
/* Draw a colinearity line between the given msp and the previous/next one in the BlxSequence's list */
static void mspDrawColinearityLineAdjacent(const MSP* msp, const gboolean selected, RenderData *data, gboolean prev)
{
if (data && msp && msp->sSequence)
{
/* Find the current msp in the list, and get the one before it */
GList *mspItem = msp->sSequence->mspList;
for ( ; mspItem; mspItem = mspItem->next)
{
if (mspItem->data == msp)
break;
}
if (mspItem) /* found it */
{
GList *adjacentItem = (prev ? mspItem->prev : mspItem->next);
if (adjacentItem)
{
const MSP* adjacentMsp = (const MSP*)(adjacentItem->data);
if (prev)
mspDrawColinearityLine(adjacentMsp, msp, selected, data);
else
mspDrawColinearityLine(msp, adjacentMsp, selected, data);
}
}
}
}
/* Draw colinearity lines between the two given, adjacent MSPs in the same row, if applicable */
static void mspDrawColinearityLines(const MSP* cur_msp, const MSP* prev_msp, const gboolean selected, RenderData *data)
{
g_return_if_fail(data);
if (data && data->bc && data->bc->flags[BLXFLAG_SHOW_COLINEARITY] &&
(selected || !data->bc->flags[BLXFLAG_SHOW_COLINEARITY_SELECTED]))
{
if (data->bc->seqType == BLXSEQ_DNA)
{
/* Draw a line between the adjacent MSPs*/
mspDrawColinearityLine(prev_msp, cur_msp, selected, data);
/* If it's the first MSP in the row, then check for adjacent MSPs in the sequence that may be
* in different rows. */
if (prev_msp == NULL)
mspDrawColinearityLineAdjacent(cur_msp, selected, data, TRUE);
/* If it's the last MSP in the row, then check for adjacent MSPs in the sequence that may be
* in different rows. */
if (cur_msp == NULL)
mspDrawColinearityLineAdjacent(prev_msp, selected, data, FALSE);
}
else
{
/* Protein mode. The two given MSPs are in the same row and hence the same frame, but
* we therefore can't assume they're adjacent because the real next/previous MSP might
* be in a different frame. Therefore we have to draw the line to the previous AND next
* MSP for every MSP (i.e. we draw the joining lines twice; if they are in the same frame
* they will overlap but otherwise they need to be shown in each frame) */
if (cur_msp)
mspDrawColinearityLineAdjacent(cur_msp, selected, data, TRUE);
if (prev_msp)
mspDrawColinearityLineAdjacent(prev_msp, selected, data, FALSE);
}
}
}
/* There can be multiple MSPs in the same cell. This function loops through them
* and draws each one (IF it is in the correct strand/frame for this tree). */
static void rendererDrawMsps(SequenceCellRenderer *renderer,
GtkWidget *tree,
GdkWindow *window,
GtkStateType state,
GdkRectangle *cell_area)
{
/* Extract all the info from the tree that we'll need repeatedly. */
TreeProperties *treeProperties = treeGetProperties(tree);
DetailViewProperties *detailViewProperties = detailViewGetProperties(treeProperties->detailView);
BlxContext *bc = blxWindowGetContext(detailViewProperties->blxWindow());
const gboolean highlightDiffs = bc->flags[BLXFLAG_HIGHLIGHT_DIFFS]; /* swap match/mismatch colors if this is true */
const MSP *firstMsp = (const MSP*)(renderer->mspGList->data);
const BlxSequence *seq = firstMsp ? firstMsp->sSequence : NULL;
GdkColor *matchColor = getGdkColor(BLXCOLOR_MATCH, bc->defaultColors, FALSE, bc->usePrintColors);
GdkColor *matchColorSelected = getGdkColor(BLXCOLOR_MATCH, bc->defaultColors, TRUE, bc->usePrintColors);
GdkColor *mismatchColor = getGdkColor(BLXCOLOR_MISMATCH, bc->defaultColors, FALSE, bc->usePrintColors);
GdkColor *mismatchColorSelected = getGdkColor(BLXCOLOR_MISMATCH, bc->defaultColors, TRUE, bc->usePrintColors);
GdkColor *backgroundColorSelected = getGdkColor(BLXCOLOR_BACKGROUND, bc->defaultColors, TRUE, bc->usePrintColors);
GdkGC *gc = gdk_gc_new(window);
/* Make the dashes of the partial-boundary lines very short and closely
* packed (i.e. dash length of 2 pixels and gaps of 1 pixel) */
int listLen = 2;
gint8 dashList[listLen];
dashList[0] = 2;
dashList[1] = 1;
gdk_gc_set_dashes(gc, 1, dashList, listLen);
RenderData data = {
bc,
cell_area,
window,
state,
gc,
treeGetStrand(tree),
treeProperties->readingFrame,
detailViewGetSelectedDisplayIdx(treeProperties->detailView),
detailViewGetSelectedDisplayIdxRange(treeProperties->detailView),
blxWindowIsSeqSelected(detailViewProperties->blxWindow(), seq),
blxWindowGetSelectedSeqs(detailViewProperties->blxWindow()),
detailViewProperties->cellXPadding,
detailViewProperties->cellYPadding,
detailViewProperties->charWidth(),
detailViewProperties->charHeight(),
&detailViewProperties->displayRange,
highlightDiffs,
widgetGetDrawable(tree),
detailViewProperties->blxWindow(),
getGdkColor(BLXCOLOR_EXON_FILL, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_EXON_FILL, bc->defaultColors, TRUE, bc->usePrintColors),
getGdkColor(BLXCOLOR_CDS_FILL, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_CDS_FILL, bc->defaultColors, TRUE, bc->usePrintColors),
getGdkColor(BLXCOLOR_UTR_FILL, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_UTR_FILL, bc->defaultColors, TRUE, bc->usePrintColors),
getGdkColor(BLXCOLOR_PARTIAL_EXON_CROSSHATCH, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_PARTIAL_EXON_CROSSHATCH, bc->defaultColors, TRUE, bc->usePrintColors),
getGdkColor(BLXCOLOR_INSERTION, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_INSERTION, bc->defaultColors, TRUE, bc->usePrintColors),
highlightDiffs ? mismatchColor : matchColor,
highlightDiffs ? mismatchColorSelected : matchColorSelected,
getGdkColor(BLXCOLOR_CONS, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_CONS, bc->defaultColors, TRUE, bc->usePrintColors),
highlightDiffs ? matchColor : mismatchColor,
highlightDiffs ? matchColorSelected : mismatchColorSelected,
getGdkColor(BLXCOLOR_UNALIGNED_SEQ, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_UNALIGNED_SEQ, bc->defaultColors, TRUE, bc->usePrintColors),
getGdkColor(BLXCOLOR_EXON_START, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_EXON_END, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_POLYA_TAIL, bc->defaultColors, FALSE, bc->usePrintColors),
getGdkColor(BLXCOLOR_POLYA_TAIL, bc->defaultColors, TRUE, bc->usePrintColors),
getGdkColor(BLXCOLOR_CLIP_MARKER, bc->defaultColors, FALSE, bc->usePrintColors),
detailViewProperties->exonBoundaryLineWidth,
detailViewProperties->exonBoundaryLineStyle,
detailViewProperties->exonBoundaryLineStylePartial,
bc->flags[BLXFLAG_LIMIT_UNALIGNED_BASES],
detailViewProperties->numUnalignedBases
};
/* If a range is selected highlight it now in case we don't come to process it (in
* which case this will get drawn over). */
if (data.selectionRange)
{
int i = 0;
for (i = data.selectionRange->min(); i <= data.selectionRange->max(); ++i)
{
if (coordIsSelected(&data, i))
highlightSelectedBase(i, backgroundColorSelected, &data);
}
}
/* Draw all MSPs in this row */
GList *mspListItem = renderer->mspGList;
MSP *savedMsp = NULL;
MSP *prevMsp = NULL;
for ( ; mspListItem; mspListItem = mspListItem->next)
{
MSP *msp = (MSP*)(mspListItem->data);
if (mspIsBlastMatch(msp))
{
gboolean selected = blxWindowIsSeqSelected(detailViewProperties->blxWindow(), msp->sSequence);
if (mspGetFlag(msp, MSPFLAG_SQUASH_IDENTICAL_FEATURES) && !mspGetFlag(msp, MSPFLAG_SQUASH_LINKED_FEATURES))
{
/* The first condition here means that identical matches are placed in the
* same row and the second means that matches linked in any other way aren't. Hence
* if we get here we know that if we have multiple MSPs in this row then they
* must be identical (i.e. duplicate) matches and we only need to draw one of them.
* This is an important optimisation for BAM data, where we can have hundreds of
* identical reads on the same row.
* gb10 2014: Ideally we should add optimisation for the case where we have both
* linked features and identical matches in the same row, but this isn't used at the
* moment. */
/* If any of the MSPs is selected, we want to draw the row as selected, so loop through
* checking if any are selected. If a selected one is found then draw it; otherwise,
* save the first MSP so we can go back and draw that. */
if (selected)
{
data.seqSelected = TRUE;
drawMsp(renderer, msp, tree, &data);
savedMsp = NULL;
break;
}
else if (!savedMsp)
{
savedMsp = msp;
}
}
else
{
/* Ordinary row: draw all MSPs */
drawMsp(renderer, msp, tree, &data);
mspDrawColinearityLines(msp, prevMsp, selected, &data);
}
}
else if (mspIsBoxFeature(msp))
{
drawBoxFeature(renderer, msp, tree, &data);
}
prevMsp = msp;
}
/* Draw colinearity lines for the last msp. Passing curMsp as null indicates it's the last msp
* so we search for adjacent msps in different rows. */
if (prevMsp)
mspDrawColinearityLines(NULL, prevMsp, blxWindowIsSeqSelected(detailViewProperties->blxWindow(), prevMsp->sSequence), &data);
if (savedMsp)
drawMsp(renderer, savedMsp, tree, &data);
drawAllVisibleExonBoundaries(tree, &data);
g_object_unref(gc);
if (data.selectionRange)
{
delete data.selectionRange;
data.selectionRange = NULL;
}
}
static GtkStateType getState(GtkWidget *widget, GtkCellRendererState flags)
{
GtkStateType state;
if ((flags & GTK_CELL_RENDERER_SELECTED) == GTK_CELL_RENDERER_SELECTED)
{
state = GTK_WIDGET_HAS_FOCUS(widget) ? GTK_STATE_SELECTED : GTK_STATE_ACTIVE;
}
else if ((flags & GTK_CELL_RENDERER_PRELIT) == GTK_CELL_RENDERER_PRELIT && GTK_WIDGET_STATE (widget) == GTK_STATE_PRELIGHT)
{
state = GTK_STATE_PRELIGHT;
}
else if (GTK_WIDGET_STATE (widget) == GTK_STATE_INSENSITIVE)
{
state = GTK_STATE_INSENSITIVE;
}
else
{
state = GTK_STATE_NORMAL;
}
return state;
}
/* Utility function that returns true if any of the MSPs in the given list
* is selected. */
static gboolean mspListContainsSelectedMsp(GList *mspList, const BlxContext* const bc)
{
gboolean isSelected = FALSE;
GList *mspItem = mspList;
for ( ; mspItem && !isSelected; mspItem = mspItem->next)
{
const MSP* const msp = (const MSP*)(mspItem->data);
isSelected = bc->isSeqSelected(msp->sSequence);
}
return isSelected;
}
/* Utility to determine if any MSP in the given list is in a group and, if so
* to return that group. Returns the first group found and ignores any
* subsequent MSPs in the list that also have groups. Returns null if no group
* was found. */
static SequenceGroup* mspListContainsGroupedMsp(GList *mspList, const BlxContext* const bc)
{
SequenceGroup *group = NULL;
GList *mspItem = mspList;
for ( ; mspItem && !group; mspItem = mspItem->next)
{
const MSP* const msp = (const MSP*)(mspItem->data);
group = bc->getFirstSequenceGroup(msp->sSequence);
}
return group;
}
/* This function sets the background color for the row. */
static void rendererSetBackgroundColor(GtkCellRenderer *cell, GtkWidget *tree, GdkWindow *window, GdkRectangle *background_area)
{
SequenceCellRenderer *renderer = SEQUENCE_CELL_RENDERER(cell);
if (renderer->data)
{
/* Find out whether the MSP(s) that this cell is displaying are in
* a grouped sequence or are selected. */
const BlxContext* const bc = blxWindowGetContext(treeGetBlxWindow(tree));
GList *mspList = renderer->data;
const gboolean isSelected = mspListContainsSelectedMsp(mspList, bc);
const SequenceGroup* const group = mspListContainsGroupedMsp(mspList, bc);
GdkGC *gc = gdk_gc_new(window);
if (isSelected || (group && group->highlighted))
{
if (group && group->highlighted && isSelected)
{
/* Use the group's highlight color but darken it because it is also selected */
GdkColor color;
getSelectionColor(&group->highlightColor, &color);
gdk_gc_set_foreground(gc, &color);
}
else if (group && group->highlighted)
{
/* Use the group's highlight color */
gdk_gc_set_foreground(gc, &group->highlightColor);
}
else
{
/* Not in a group but is selected. Use the background color but darken it. */
GdkColor color;
getSelectionColor(&tree->style->bg[GTK_STATE_NORMAL], &color);
gdk_gc_set_foreground(gc, &color);
}
}
else
{
gdk_gc_set_foreground(gc, &tree->style->bg[GTK_STATE_NORMAL]);
}
drawRectangle2(window, widgetGetDrawable(tree), gc, TRUE, background_area->x, background_area->y, background_area->width, background_area->height);
g_object_unref(gc);
}
}
/* Draw the tree grid lines (i.e. column separators) */
static void rendererDrawGridLines(GtkWidget *tree, GdkWindow *window, GdkRectangle *cell_area)
{
GdkDrawable *drawable = widgetGetDrawable(tree);
BlxContext *bc = treeGetContext(tree);
GdkGC *gc = gdk_gc_new(window);
/* Set the line color */
GdkColor *color = getGdkColor(BLXCOLOR_TREE_GRID_LINES, bc->defaultColors, FALSE, bc->usePrintColors);
gdk_gc_set_foreground(gc, color);
/* We want dashed lines */
const int lineWidth = 1;
gdk_gc_set_line_attributes(gc, lineWidth, GDK_LINE_ON_OFF_DASH, GDK_CAP_BUTT, GDK_JOIN_MITER);
/* Make the dashes very short and closely packed (i.e. dash length of 1 pixel and gaps of 1 pixel) */
int listLen = 1;
gint8 dashList[listLen];
dashList[0] = 1;
gdk_gc_set_dashes(gc, 0, dashList, listLen);
/* Draw vertical lines. Draw the right edge of each cell (because we don't really want a
* line at the leftmost edge of the first cell.) */
const int x = cell_area->x + cell_area->width - lineWidth;
drawLine2(window, drawable, gc, x, cell_area->y, x, cell_area->y + cell_area->height);
g_object_unref(gc);
}
/***************************************************************************
*
* sequence_cell_renderer_get_size: crucial - calculate the size
* of our cell, taking into account
* padding and alignment properties
* of parent.
*
***************************************************************************/
static void
sequence_cell_renderer_get_size (GtkCellRenderer *cell,
GtkWidget *widget,
GdkRectangle *cell_area,
gint *x_offset,
gint *y_offset,
gint *width,
gint *height)
{
get_size(cell, widget, cell_area, x_offset, y_offset, width, height);
}
/***************************************************************************
*
* sequence_cell_renderer_render: crucial - do the rendering.
*
***************************************************************************/
static void
sequence_cell_renderer_render (GtkCellRenderer *cell,
GdkDrawable *window,
GtkWidget *tree,
GdkRectangle *background_area,
GdkRectangle *cell_area,
GdkRectangle *expose_area,
GtkCellRendererState flags)
{
rendererSetBackgroundColor(cell, tree, window, background_area);
SequenceCellRenderer *renderer = SEQUENCE_CELL_RENDERER(cell);
MSP *msp = renderer->mspGList ? (MSP*)(renderer->mspGList->data) : NULL;
if (msp)
{
rendererDrawMsps(renderer, tree, window, getState(tree, flags), cell_area);
}
else
{
rendererDrawSimpleText(renderer, tree, window, getState(tree, flags), cell_area);
}
rendererDrawGridLines(tree, window, background_area);
}
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