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Source: smalr
Maintainer: Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.org>
Uploaders: Afif Elghraoui <afif@debian.org>
Section: science
Priority: optional
Build-Depends: debhelper (>= 11),
dh-python,
python-all,
python-setuptools,
python-pbcore,
python-h5py,
python-numpy
Standards-Version: 4.2.1
Vcs-Browser: https://salsa.debian.org/med-team/smalr
Vcs-Git: https://salsa.debian.org/med-team/smalr.git
Homepage: https://github.com/fanglab/SMALR
Package: smalr
Architecture: all
Depends: ${misc:Depends},
${python:Depends},
samtools,
bwa (>= 0.7),
r-base-core (>= 3.0),
r-cran-seqinr
Description: interrogation of the methylation status of nucleotide sequencing reads
The SMALR package conducts single-molecule level interrogation of the
methylation status of single-molecule real-time (SMRT) sequencing reads.
There are two protocols available for use within the pipeline, SMsn and SMp.
* SMsn: Single-molecule, single nucleotide analysis
Each motif site on each sequencing molecule is assessed for methylation
status. This is designed for use with short (~250bp) sequencing library
preps, where the long read lengths of SMRT reads enables multiple passes
over each motif site. The reliability of the SMsn scores increases with
more passes (i.e. higher single-molecule coverage).
* SMp: Single-molecule, motif-pooled analysis
All motif sites on a sequencing molecule are pooled together and the
molecule-wide methylation status for the given motif is assessed. This is
designed for use with long (10Kb+) sequencing library preps, where each
single long subread can span many distinct motif sites. The reliability of
the SMp scores increases with increasing number of distinct motif sites
contained in the subread.
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