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"""
Tests for `sourmash prefetch` command-line and API functionality.
"""
import os
import csv
import gzip
import pytest
import glob
import random
from sourmash.search import PrefetchResult
import sourmash_tst_utils as utils
import sourmash
from sourmash_tst_utils import SourmashCommandFailed
from sourmash import SourmashSignature, sourmash_args
from sourmash.signature import (
save_signatures_to_json,
load_signatures_from_json,
load_one_signature_from_json,
)
def approx_eq(val1, val2):
if val1 == "":
return val1 == val2
return round(float(val1), 3) == round(float(val2), 3)
def test_prefetch_basic(runtmp, linear_gather):
c = runtmp
# test a basic prefetch
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
c.run_sourmash("prefetch", "-k", "31", sig47, sig63, sig2, sig47, linear_gather)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert (
"WARNING: no output(s) specified! Nothing will be saved from this prefetch!"
in c.last_result.err
)
assert "selecting specified query k=31" in c.last_result.err
assert (
"loaded query: NC_009665.1 Shewanella baltica... (k=31, DNA)"
in c.last_result.err
)
assert (
"query sketch has scaled=1000; will be dynamically downsampled as needed"
in c.last_result.err
)
err = c.last_result.err
assert "loaded 5 total signatures from 3 locations." in err
assert "after selecting signatures compatible with search, 3 remain." in err
assert "total of 2 matching signatures." in c.last_result.err
assert (
"of 5177 distinct query hashes, 5177 were found in matches above threshold."
in c.last_result.err
)
assert "a total of 0 query hashes remain unmatched." in c.last_result.err
def test_prefetch_select_query_ksize(runtmp, linear_gather):
# test prefetch where query and subject db both have multiple ksizes
c = runtmp
ss = utils.get_test_data("GCF_000005845.2_ASM584v2_genomic.fna.gz.sig")
c.run_sourmash("prefetch", ss, ss, linear_gather)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert (
"of 4476 distinct query hashes, 4476 were found in matches above threshold."
in c.last_result.err
)
def test_prefetch_subject_scaled_is_larger(runtmp, linear_gather):
# test prefetch where subject scaled is larger
c = runtmp
# make a query sketch with scaled=1000
fa = utils.get_test_data("genome-s10.fa.gz")
c.run_sourmash("sketch", "dna", fa, "-o", "query.sig")
assert os.path.exists(runtmp.output("query.sig"))
# this has a scaled of 10000, from same genome:
against1 = utils.get_test_data("scaled/genome-s10.fa.gz.sig")
against2 = utils.get_test_data("scaled/all.sbt.zip")
against3 = utils.get_test_data("scaled/all.lca.json")
# run against large scaled, then small (self)
c.run_sourmash(
"prefetch",
"query.sig",
against1,
against2,
against3,
"query.sig",
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert "total of 8 matching signatures." in c.last_result.err
assert (
"of 48 distinct query hashes, 48 were found in matches above threshold."
in c.last_result.err
)
assert (
"final scaled value (max across query and all matches) is 10000"
in c.last_result.err
)
def test_prefetch_subject_scaled_is_larger_outsigs(runtmp, linear_gather):
# test prefetch where subject scaled is larger -- output sigs
c = runtmp
# make a query sketch with scaled=1000
fa = utils.get_test_data("genome-s10.fa.gz")
c.run_sourmash("sketch", "dna", fa, "-o", "query.sig")
assert os.path.exists(runtmp.output("query.sig"))
# this has a scaled of 10000, from same genome:
against1 = utils.get_test_data("scaled/genome-s10.fa.gz.sig")
against2 = utils.get_test_data("scaled/all.sbt.zip")
against3 = utils.get_test_data("scaled/all.lca.json")
# run against large scaled, then small (self)
c.run_sourmash(
"prefetch",
"query.sig",
against1,
against2,
against3,
"query.sig",
linear_gather,
"--save-matches",
"matches.sig",
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert "total of 8 matching signatures." in c.last_result.err
assert (
"of 48 distinct query hashes, 48 were found in matches above threshold."
in c.last_result.err
)
assert (
"final scaled value (max across query and all matches) is 10000"
in c.last_result.err
)
# make sure non-downsampled sketches were saved.
matches = sourmash.load_file_as_signatures(runtmp.output("matches.sig"))
scaled_vals = set([match.minhash.scaled for match in matches])
assert 1000 in scaled_vals
assert 10000 in scaled_vals
assert len(scaled_vals) == 2
def test_prefetch_query_abund(runtmp, linear_gather):
c = runtmp
# test a basic prefetch w/abund query
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("track_abund/47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
c.run_sourmash("prefetch", "-k", "31", sig47, sig63, sig2, sig47, linear_gather)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert (
"WARNING: no output(s) specified! Nothing will be saved from this prefetch!"
in c.last_result.err
)
assert "selecting specified query k=31" in c.last_result.err
assert (
"loaded query: NC_009665.1 Shewanella baltica... (k=31, DNA)"
in c.last_result.err
)
assert (
"query sketch has scaled=1000; will be dynamically downsampled as needed"
in c.last_result.err
)
assert "total of 2 matching signatures." in c.last_result.err
assert (
"of 5177 distinct query hashes, 5177 were found in matches above threshold."
in c.last_result.err
)
assert "a total of 0 query hashes remain unmatched." in c.last_result.err
def test_prefetch_subj_abund(runtmp, linear_gather):
c = runtmp
# test a basic prefetch w/abund signature.
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("track_abund/63.fa.sig")
c.run_sourmash("prefetch", "-k", "31", sig47, sig63, sig2, sig47, linear_gather)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert (
"WARNING: no output(s) specified! Nothing will be saved from this prefetch!"
in c.last_result.err
)
assert "selecting specified query k=31" in c.last_result.err
assert (
"loaded query: NC_009665.1 Shewanella baltica... (k=31, DNA)"
in c.last_result.err
)
assert (
"query sketch has scaled=1000; will be dynamically downsampled as needed"
in c.last_result.err
)
assert "total of 2 matching signatures." in c.last_result.err
assert (
"of 5177 distinct query hashes, 5177 were found in matches above threshold."
in c.last_result.err
)
assert "a total of 0 query hashes remain unmatched." in c.last_result.err
def test_prefetch_csv_out(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with CSV output
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
csvout = c.output("out.csv")
c.run_sourmash(
"prefetch", "-k", "31", sig47, sig63, sig2, sig47, "-o", csvout, linear_gather
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(csvout)
expected_intersect_bp = [2529000, 5177000]
with open(csvout, newline="") as fp:
r = csv.DictReader(fp)
for row, expected in zip(r, expected_intersect_bp):
print(row)
assert int(row["intersect_bp"]) == expected
def test_prefetch_csv_gz_out(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with CSV output to a .gz file
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
csvout = c.output("out.csv.gz")
c.run_sourmash(
"prefetch", "-k", "31", sig47, sig63, sig2, sig47, "-o", csvout, linear_gather
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(csvout)
expected_intersect_bp = [2529000, 5177000]
with gzip.open(csvout, "rt", newline="") as fp:
r = csv.DictReader(fp)
for row, expected in zip(r, expected_intersect_bp):
print(row)
assert int(row["intersect_bp"]) == expected
def test_prefetch_matches(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with --save-matches
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
matches_out = c.output("matches.sig")
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--save-matches",
matches_out,
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(matches_out)
sigs = sourmash.load_file_as_index(matches_out)
expected_matches = [sig63, sig47]
for match, expected in zip(sigs.signatures(), expected_matches):
ss = load_one_signature_from_json(expected, ksize=31)
assert match == ss
def test_prefetch_matches_to_dir(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with --save-matches to a directory
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
ss63 = load_one_signature_from_json(sig63)
ss47 = load_one_signature_from_json(sig47)
matches_out = c.output("matches_dir/")
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--save-matches",
matches_out,
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(matches_out)
assert os.path.isdir(matches_out)
sigs = sourmash.load_file_as_signatures(matches_out)
match_sigs = list(sigs)
assert ss63 in match_sigs
assert ss47 in match_sigs
assert len(match_sigs) == 2
def test_prefetch_matches_to_sig_gz(runtmp, linear_gather):
c = runtmp
import gzip
# test a basic prefetch, with --save-matches to a sig.gz file
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
ss63 = load_one_signature_from_json(sig63)
ss47 = load_one_signature_from_json(sig47)
matches_out = c.output("matches.sig.gz")
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--save-matches",
matches_out,
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(matches_out)
assert os.path.isfile(matches_out)
with gzip.open(matches_out, "rt") as fp:
# can we read this as a gz file?
fp.read()
sigs = sourmash.load_file_as_signatures(matches_out)
match_sigs = list(sigs)
assert ss63 in match_sigs
assert ss47 in match_sigs
assert len(match_sigs) == 2
def test_prefetch_matches_to_zip(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with --save-matches to a zipfile
import zipfile
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
ss63 = load_one_signature_from_json(sig63)
ss47 = load_one_signature_from_json(sig47)
matches_out = c.output("matches.zip")
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--save-matches",
matches_out,
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(matches_out)
assert os.path.isfile(matches_out)
with zipfile.ZipFile(matches_out, "r") as fp:
# can we read this as a .zip file?
for zi in fp.infolist():
pass
sigs = sourmash.load_file_as_signatures(matches_out)
match_sigs = list(sigs)
assert ss63 in match_sigs
assert ss47 in match_sigs
assert len(match_sigs) == 2
def test_prefetch_matching_hashes(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with --save-matches
utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
matches_out = c.output("matches.sig")
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
"--save-matching-hashes",
matches_out,
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(matches_out)
ss47 = load_one_signature_from_json(sig47, ksize=31)
ss63 = load_one_signature_from_json(sig63, ksize=31)
matches = set(ss47.minhash.hashes) & set(ss63.minhash.hashes)
intersect = ss47.minhash.copy_and_clear()
intersect.add_many(matches)
ss = load_one_signature_from_json(matches_out)
assert ss.name.endswith("-known")
assert ss.minhash == intersect
def test_prefetch_nomatch_hashes(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with --save-matches
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
nomatch_out = c.output("unmatched_hashes.sig")
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
"--save-unmatched-hashes",
nomatch_out,
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(nomatch_out)
ss47 = load_one_signature_from_json(sig47, ksize=31)
ss63 = load_one_signature_from_json(sig63, ksize=31)
remain = ss47.minhash.to_mutable()
remain.remove_many(ss63.minhash.hashes)
ss = load_one_signature_from_json(nomatch_out)
assert ss.name.endswith("-unknown")
assert ss.minhash == remain
def test_prefetch_no_num_query(runtmp, linear_gather):
c = runtmp
# can't do prefetch with num signatures for query
sig47 = utils.get_test_data("num/47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
with pytest.raises(SourmashCommandFailed):
c.run_sourmash("prefetch", "-k", "31", sig47, sig63, sig47, linear_gather)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status != 0
def test_prefetch_no_num_subj(runtmp, linear_gather):
c = runtmp
# can't do prefetch with num signatures for query; no matches!
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("num/63.fa.sig")
with pytest.raises(SourmashCommandFailed):
c.run_sourmash("prefetch", "-k", "31", sig47, sig63, linear_gather)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status != 0
assert (
"ERROR in prefetch: after picklists and patterns, no signatures to search!?"
in c.last_result.err
)
def test_prefetch_db_fromfile(runtmp, linear_gather):
c = runtmp
# test a basic prefetch
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
from_file = c.output("from-list.txt")
with open(from_file, "w") as fp:
print(sig63, file=fp)
print(sig2, file=fp)
print(sig47, file=fp)
c.run_sourmash(
"prefetch", "-k", "31", sig47, linear_gather, "--db-from-file", from_file
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert (
"WARNING: no output(s) specified! Nothing will be saved from this prefetch!"
in c.last_result.err
)
assert "selecting specified query k=31" in c.last_result.err
assert (
"loaded query: NC_009665.1 Shewanella baltica... (k=31, DNA)"
in c.last_result.err
)
assert (
"query sketch has scaled=1000; will be dynamically downsampled as needed"
in c.last_result.err
)
assert "total of 2 matching signatures." in c.last_result.err
assert (
"of 5177 distinct query hashes, 5177 were found in matches above threshold."
in c.last_result.err
)
assert "a total of 0 query hashes remain unmatched." in c.last_result.err
def test_prefetch_no_db(runtmp, linear_gather):
c = runtmp
# test a basic prefetch with no databases/signatures
sig47 = utils.get_test_data("47.fa.sig")
with pytest.raises(SourmashCommandFailed):
c.run_sourmash("prefetch", "-k", "31", sig47, linear_gather)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status != 0
assert "ERROR: no databases or signatures to search!?" in c.last_result.err
def test_prefetch_check_scaled_bounds_negative(runtmp, linear_gather):
c = runtmp
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
with pytest.raises(SourmashCommandFailed) as exc:
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--scaled",
"-5",
linear_gather,
)
assert "ERROR: scaled value must be positive" in str(exc.value)
def test_prefetch_check_scaled_bounds_less_than_minimum(runtmp, linear_gather):
c = runtmp
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
with pytest.raises(SourmashCommandFailed) as exc:
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--scaled",
"50",
linear_gather,
)
assert "WARNING: scaled value should be >= 100. Continuing anyway." in str(
exc.value
)
def test_prefetch_check_scaled_bounds_more_than_maximum(runtmp, linear_gather):
c = runtmp
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
with pytest.raises(SourmashCommandFailed) as exc:
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--scaled",
"1e9",
linear_gather,
)
assert "WARNING: scaled value should be <= 1e6. Continuing anyway." in str(
exc.value
)
def test_prefetch_downsample_scaled(runtmp, linear_gather):
c = runtmp
# test --scaled
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--scaled",
"1e5",
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert "downsampling query from scaled=1000 to 10000" in c.last_result.err
def test_prefetch_downsample_multiple(runtmp, linear_gather):
# test multiple different downsamplings in prefetch code
query_sig = utils.get_test_data("GCF_000006945.2-s500.sig")
# load in the hashes and do split them into four bins, randomly.
ss = load_one_signature_from_json(query_sig)
hashes = list(ss.minhash.hashes)
random.seed(a=1) # fix seed so test is reproducible
random.shuffle(hashes)
# split into 4 bins:
mh_bins = [ss.minhash.copy_and_clear() for i in range(4)]
for i, hashval in enumerate(hashes):
mh_bins[i % 4].add_hash(hashval)
# downsample with different scaleds; initial scaled is 500, note.
mh_bins[0] = mh_bins[0].downsample(scaled=750)
mh_bins[1] = mh_bins[1].downsample(scaled=600)
mh_bins[2] = mh_bins[2].downsample(scaled=1000)
mh_bins[3] = mh_bins[3].downsample(scaled=650)
gathersigs = []
for i in range(4):
binsig = SourmashSignature(mh_bins[i], name=f"bin{i}")
with open(runtmp.output(f"bin{i}.sig"), "wb") as fp:
save_signatures_to_json([binsig], fp)
gathersigs.append(f"bin{i}.sig")
runtmp.sourmash("prefetch", linear_gather, query_sig, *gathersigs)
print(runtmp.last_result.out)
print(runtmp.last_result.err)
assert (
"final scaled value (max across query and all matches) is 1000"
in runtmp.last_result.err
)
def test_prefetch_empty(runtmp, linear_gather):
c = runtmp
# test --scaled
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
with pytest.raises(SourmashCommandFailed):
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"--scaled",
"1e9",
linear_gather,
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status != 0
assert "no query hashes!? exiting." in c.last_result.err
def test_prefetch_basic_many_sigs(runtmp, linear_gather):
c = runtmp
# test what happens with many (and duplicate) signatures
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
manysigs = [sig63, sig2, sig47] * 5
c.run_sourmash("prefetch", "-k", "31", sig47, *manysigs, linear_gather)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert "total of 10 matching signatures so far." in c.last_result.err
assert "total of 10 matching signatures." in c.last_result.err
assert (
"of 5177 distinct query hashes, 5177 were found in matches above threshold."
in c.last_result.err
)
assert "a total of 0 query hashes remain unmatched." in c.last_result.err
def test_prefetch_with_picklist(runtmp):
# test 'sourmash prefetch' with picklists
gcf_sigs = glob.glob(utils.get_test_data("gather/GCF*.sig"))
metag_sig = utils.get_test_data("gather/combined.sig")
picklist = utils.get_test_data("gather/thermotoga-picklist.csv")
runtmp.sourmash(
"prefetch", metag_sig, *gcf_sigs, "--picklist", f"{picklist}:md5:md5"
)
err = runtmp.last_result.err
print(err)
assert "for given picklist, found 3 matches to 9 distinct values" in err
# these are the different ksizes
assert "WARNING: 6 missing picklist values." in err
out = runtmp.last_result.out
print(out)
assert "total of 3 matching signatures." in err
assert (
"of 1466 distinct query hashes, 453 were found in matches above threshold."
in err
)
assert "a total of 1013 query hashes remain unmatched." in err
def test_prefetch_with_picklist_exclude(runtmp):
# test 'sourmash prefetch' with picklists, exclude
gcf_sigs = glob.glob(utils.get_test_data("gather/GCF*.sig"))
metag_sig = utils.get_test_data("gather/combined.sig")
picklist = utils.get_test_data("gather/thermotoga-picklist.csv")
runtmp.sourmash(
"prefetch", metag_sig, *gcf_sigs, "--picklist", f"{picklist}:md5:md5:exclude"
)
err = runtmp.last_result.err
print(err)
assert "for given picklist, found 9 matches by excluding 9 distinct values" in err
# these are the different ksizes
out = runtmp.last_result.out
print(out)
assert "total of 9 matching signatures." in err
assert (
"of 1466 distinct query hashes, 1013 were found in matches above threshold."
in err
)
assert "a total of 453 query hashes remain unmatched." in err
def test_prefetch_with_pattern_include(runtmp):
# test 'sourmash prefetch' with --include-db-pattern
gcf_sigs = glob.glob(utils.get_test_data("gather/GCF*.sig"))
metag_sig = utils.get_test_data("gather/combined.sig")
runtmp.sourmash("prefetch", metag_sig, *gcf_sigs, "--include", "thermotoga")
err = runtmp.last_result.err
print(err)
out = runtmp.last_result.out
print(out)
assert "total of 3 matching signatures." in err
assert (
"of 1466 distinct query hashes, 453 were found in matches above threshold."
in err
)
assert "a total of 1013 query hashes remain unmatched." in err
def test_prefetch_with_pattern_exclude(runtmp):
# test 'sourmash prefetch' with --exclude-db-pattern
gcf_sigs = glob.glob(utils.get_test_data("gather/GCF*.sig"))
metag_sig = utils.get_test_data("gather/combined.sig")
runtmp.sourmash("prefetch", metag_sig, *gcf_sigs, "--exclude", "thermotoga")
err = runtmp.last_result.err
print(err)
out = runtmp.last_result.out
print(out)
assert "total of 9 matching signatures." in err
assert (
"of 1466 distinct query hashes, 1013 were found in matches above threshold."
in err
)
assert "a total of 453 query hashes remain unmatched." in err
def test_prefetch_output_with_abundance(runtmp, prefetch_gather, linear_gather):
c = runtmp
query = utils.get_test_data("gather-abund/reads-s10x10-s11.sig")
against = utils.get_test_data("gather-abund/genome-s10.fa.gz.sig")
c.run_sourmash(
"prefetch",
linear_gather,
query,
against,
"--save-matching-hashes",
c.output("match-hash.sig"),
"--save-unmatched-hashes",
c.output("nomatch-hash.sig"),
)
print(c.last_result.out)
assert os.path.exists(c.output("match-hash.sig"))
ss = list(sourmash.load_file_as_signatures(c.output("match-hash.sig")))[0]
assert ss.minhash.track_abundance
assert os.path.exists(c.output("nomatch-hash.sig"))
ss = list(sourmash.load_file_as_signatures(c.output("nomatch-hash.sig")))[0]
assert ss.minhash.track_abundance
def test_prefetch_ani_csv_out(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with CSV output
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
csvout = c.output("out.csv")
c.run_sourmash(
"prefetch", "-k", "31", sig47, sig63, sig2, sig47, "-o", csvout, linear_gather
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(csvout)
prefetch_result_names = PrefetchResult.prefetch_write_cols
exp1 = {
"q_ani": "0.9771552502238963",
"m_ani": "0.9767860811200507",
"ac_ani": "0.9769706656719734",
"mc_ani": "0.9771552502238963",
"pfn": "False",
}
exp2 = {
"q_ani": "1.0",
"m_ani": "1.0",
"ac_ani": "1.0",
"mc_ani": "1.0",
"pfn": "False",
}
expected_ani_vals = [exp1, exp2]
with open(csvout, newline="") as fp:
r = csv.DictReader(fp)
for row, expected in zip(r, expected_ani_vals):
print(row)
assert prefetch_result_names == list(row.keys())
assert approx_eq(row["query_containment_ani"], expected["q_ani"])
assert approx_eq(row["match_containment_ani"], expected["m_ani"])
assert approx_eq(row["max_containment_ani"], expected["mc_ani"])
assert approx_eq(row["average_containment_ani"], expected["ac_ani"])
assert row["potential_false_negative"] == expected["pfn"]
def test_prefetch_ani_csv_out_estimate_ci(runtmp, linear_gather):
c = runtmp
# test a basic prefetch, with CSV output
sig2 = utils.get_test_data("2.fa.sig")
sig47 = utils.get_test_data("47.fa.sig")
sig63 = utils.get_test_data("63.fa.sig")
csvout = c.output("out.csv")
c.run_sourmash(
"prefetch",
"-k",
"31",
sig47,
sig63,
sig2,
sig47,
"-o",
csvout,
linear_gather,
"--estimate-ani-ci",
)
print(c.last_result.status)
print(c.last_result.out)
print(c.last_result.err)
assert c.last_result.status == 0
assert os.path.exists(csvout)
prefetch_result_names_ci = PrefetchResult.prefetch_write_cols_ci
exp1 = {
"q_ani": "0.9771552502238963",
"m_ani": "0.9767860811200507",
"q_ani_low": "0.9762537506990911",
"q_ani_high": "0.9780336875157754",
"m_ani_low": "0.9758801604653301",
"m_ani_high": "0.9776692390768575",
"ac_ani": "0.9769706656719734",
"mc_ani": "0.9771552502238963",
"pfn": "False",
}
exp2 = {
"q_ani": "1.0",
"m_ani": "1.0",
"q_ani_low": "1.0",
"q_ani_high": "1.0",
"m_ani_low": "1.0",
"m_ani_high": "1.0",
"ac_ani": "1.0",
"mc_ani": "1.0",
"pfn": "False",
}
expected_ani_vals = [exp1, exp2]
with open(csvout, newline="") as fp:
r = csv.DictReader(fp)
for row, expected in zip(r, expected_ani_vals):
print(row)
assert prefetch_result_names_ci == list(row.keys())
assert approx_eq(row["query_containment_ani"], expected["q_ani"])
assert approx_eq(row["query_containment_ani_low"], expected["q_ani_low"])
assert approx_eq(row["query_containment_ani_high"], expected["q_ani_high"])
assert approx_eq(row["match_containment_ani"], expected["m_ani"])
assert approx_eq(row["match_containment_ani_low"], expected["m_ani_low"])
assert approx_eq(row["match_containment_ani_high"], expected["m_ani_high"])
assert approx_eq(row["max_containment_ani"], expected["mc_ani"])
assert approx_eq(row["average_containment_ani"], expected["ac_ani"])
assert row["potential_false_negative"] == expected["pfn"]
def test_prefetch_ani_containment_asymmetry(runtmp):
# test contained_by asymmetries, viz #2215
query_sig = utils.get_test_data("47.fa.sig")
merged_sig = utils.get_test_data("47-63-merge.sig")
runtmp.sourmash("prefetch", query_sig, merged_sig, "-o", "query-in-merged.csv")
runtmp.sourmash("prefetch", merged_sig, query_sig, "-o", "merged-in-query.csv")
with sourmash_args.FileInputCSV(runtmp.output("query-in-merged.csv")) as r:
query_in_merged = list(r)[0]
with sourmash_args.FileInputCSV(runtmp.output("merged-in-query.csv")) as r:
merged_in_query = list(r)[0]
assert query_in_merged["query_containment_ani"] == "1.0"
assert query_in_merged["match_containment_ani"] == "0.9865155060423993"
assert query_in_merged["average_containment_ani"] == "0.9932577530211997"
assert merged_in_query["match_containment_ani"] == "1.0"
assert merged_in_query["query_containment_ani"] == "0.9865155060423993"
assert merged_in_query["average_containment_ani"] == "0.9932577530211997"
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