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//***************************************************************************
//* Copyright (c) 2015 Saint Petersburg State University
//* Copyright (c) 2011-2014 Saint Petersburg Academic University
//* All Rights Reserved
//* See file LICENSE for details.
//***************************************************************************
#pragma once
#include "statistics.hpp"
#include "assembly_graph/core/graph.hpp"
#include "pipeline/graph_pack.hpp"
#include "modules/alignment/sequence_mapper.hpp"
#include "pipeline/graphio.hpp"
//FIXME awful dependency to get write_lib_data
#include "pipeline/config_struct.hpp"
#include "visualization/position_filler.hpp"
#include "visualization/visualization.hpp"
#include "assembly_graph/handlers/edges_position_handler.hpp"
#include "assembly_graph/components/graph_component.hpp"
#include "io/reads/rc_reader_wrapper.hpp"
#include "io/reads/delegating_reader_wrapper.hpp"
#include "io/reads/io_helper.hpp"
#include "io/reads/wrapper_collection.hpp"
#include "io/reads/osequencestream.hpp"
#include "io/dataset_support/dataset_readers.hpp"
#include "utils/filesystem/copy_file.hpp"
#include <boost/algorithm/string.hpp>
#include <sys/types.h>
#include <sys/stat.h>
#include <cmath>
namespace debruijn_graph {
namespace stats {
template<class Graph, class Index>
MappingPath<typename Graph::EdgeId>
FindGenomeMappingPath(const Sequence& genome, const Graph& g,
const Index& index,
const KmerMapper<Graph>& kmer_mapper) {
BasicSequenceMapper<Graph, Index> srt(g, index, kmer_mapper);
return srt.MapSequence(genome);
}
template<class graph_pack>
MappingPath<typename graph_pack::graph_t::EdgeId>
FindGenomeMappingPath(const Sequence& genome, const graph_pack& gp) {
return FindGenomeMappingPath(genome, gp.g, gp.index, gp.kmer_mapper);
}
template <class graph_pack>
shared_ptr<visualization::graph_colorer::GraphColorer<Graph>> DefaultColorer(const graph_pack& gp) {
return visualization::graph_colorer::DefaultColorer(gp.g,
FindGenomeMappingPath(gp.genome.GetSequence(), gp.g, gp.index, gp.kmer_mapper).path(),
FindGenomeMappingPath(!gp.genome.GetSequence(), gp.g, gp.index, gp.kmer_mapper).path());
}
template <class graph_pack>
void CollectContigPositions(graph_pack &gp) {
if (!cfg::get().pos.contigs_for_threading.empty() &&
fs::FileExists(cfg::get().pos.contigs_for_threading))
visualization::position_filler::FillPos(gp, cfg::get().pos.contigs_for_threading, "thr_", true);
if (!cfg::get().pos.contigs_to_analyze.empty() &&
fs::FileExists(cfg::get().pos.contigs_to_analyze))
visualization::position_filler::FillPos(gp, cfg::get().pos.contigs_to_analyze, "anlz_", true);
}
template<class Graph, class Index>
class GenomeMappingStat: public AbstractStatCounter {
private:
typedef typename Graph::EdgeId EdgeId;
const Graph &graph_;
const Index& index_;
Sequence genome_;
size_t k_;
public:
GenomeMappingStat(const Graph &graph, const Index &index, GenomeStorage genome, size_t k) :
graph_(graph), index_(index), genome_(genome.GetSequence()), k_(k) {}
virtual ~GenomeMappingStat() {}
virtual void Count() {
INFO("Mapping genome");
size_t break_number = 0;
size_t covered_kp1mers = 0;
size_t fail = 0;
if (genome_.size() <= k_)
return;
RtSeq cur = genome_.start<RtSeq>(k_ + 1);
cur >>= 0;
bool breaked = true;
pair<EdgeId, size_t> cur_position;
for (size_t cur_nucl = k_; cur_nucl < genome_.size(); cur_nucl++) {
cur <<= genome_[cur_nucl];
if (index_.contains(cur)) {
pair<EdgeId, size_t> next = index_.get(cur);
if (!breaked
&& cur_position.second + 1
< graph_.length(cur_position.first)) {
if (next.first != cur_position.first
|| cur_position.second + 1 != next.second) {
fail++;
}
}
cur_position = next;
covered_kp1mers++;
breaked = false;
} else {
if (!breaked) {
breaked = true;
break_number++;
}
}
}
INFO("Genome mapped");
INFO("Genome mapping results:");
INFO("Covered k+1-mers:" << covered_kp1mers << " of " << (genome_.size() - k_) << " which is "
<< (100.0 * (double) covered_kp1mers / (double) (genome_.size() - k_)) << "%");
INFO("Covered k+1-mers form " << break_number + 1 << " contiguous parts");
INFO("Continuity failtures " << fail);
}
};
template<class Graph>
void WriteErrorLoc(const Graph &g,
const string& folder_name,
std::shared_ptr<visualization::graph_colorer::GraphColorer<Graph>> genome_colorer,
const visualization::graph_labeler::GraphLabeler<Graph>& labeler) {
INFO("Writing error localities for graph to folder " << folder_name);
auto all = GraphComponent<Graph>::WholeGraph(g);
set<typename Graph::EdgeId> edges = genome_colorer->ColoredWith(all.edges().begin(),
all.edges().end(), "black");
set<typename Graph::VertexId> to_draw;
for (auto it = edges.begin(); it != edges.end(); ++it) {
to_draw.insert(g.EdgeEnd(*it));
to_draw.insert(g.EdgeStart(*it));
}
shared_ptr<GraphSplitter<Graph>> splitter = StandardSplitter(g, to_draw);
visualization::visualization_utils::WriteComponents(g, folder_name, splitter, genome_colorer, labeler);
INFO("Error localities written written to folder " << folder_name);
}
template<class graph_pack>
void CountStats(const graph_pack& gp) {
typedef typename graph_pack::graph_t Graph;
typedef typename Graph::EdgeId EdgeId;
INFO("Counting stats");
StatList stats;
Path<EdgeId> path1 = FindGenomeMappingPath(gp.genome.GetSequence(), gp.g, gp.index,
gp.kmer_mapper).path();
Path<EdgeId> path2 = FindGenomeMappingPath(!gp.genome.GetSequence(), gp.g, gp.index,
gp.kmer_mapper).path();
stats.AddStat(new VertexEdgeStat<Graph>(gp.g));
stats.AddStat(new BlackEdgesStat<Graph>(gp.g, path1, path2));
stats.AddStat(new NStat<Graph>(gp.g, path1, 50));
stats.AddStat(new SelfComplementStat<Graph>(gp.g));
stats.AddStat(
new GenomeMappingStat<Graph, Index>(gp.g, gp.index,
gp.genome, gp.k_value));
stats.AddStat(new IsolatedEdgesStat<Graph>(gp.g, path1, path2));
stats.Count();
INFO("Stats counted");
}
template<class Graph>
void WriteGraphComponentsAlongGenome(const Graph& g,
const visualization::graph_labeler::GraphLabeler<Graph>& labeler,
const string& folder,
const Path<typename Graph::EdgeId>& path1,
const Path<typename Graph::EdgeId>& path2) {
INFO("Writing graph components along genome");
make_dir(folder);
visualization::visualization_utils::WriteComponentsAlongPath(g, path1, folder,
visualization::graph_colorer::DefaultColorer(g, path1, path2),
labeler);
INFO("Writing graph components along genome finished");
}
//todo refactoring needed: use graph pack instead!!!
template<class Graph, class Mapper>
void WriteGraphComponentsAlongContigs(const Graph& g,
Mapper &mapper,
const std::string& folder,
std::shared_ptr<visualization::graph_colorer::GraphColorer<Graph>> colorer,
const visualization::graph_labeler::GraphLabeler<Graph>& labeler) {
INFO("Writing graph components along contigs");
auto contigs_to_thread = io::EasyStream(cfg::get().pos.contigs_to_analyze, false);
contigs_to_thread->reset();
io::SingleRead read;
while (!contigs_to_thread->eof()) {
(*contigs_to_thread) >> read;
make_dir(folder + read.name());
visualization::visualization_utils::WriteComponentsAlongPath(g, mapper.MapSequence(read.sequence()).simple_path(),
folder + read.name() + "/", colorer, labeler);
}
INFO("Writing graph components along contigs finished");
}
template<class Graph>
void WriteKmerComponent(conj_graph_pack &gp, RtSeq const& kp1mer, const std::string& file,
std::shared_ptr<visualization::graph_colorer::GraphColorer<Graph>> colorer,
const visualization::graph_labeler::GraphLabeler<Graph>& labeler) {
if(!gp.index.contains(kp1mer)) {
WARN("no such kmer in the graph");
return;
}
VERIFY(gp.index.contains(kp1mer));
auto pos = gp.index.get(kp1mer);
typename Graph::VertexId v = pos.second * 2 < gp.g.length(pos.first) ? gp.g.EdgeStart(pos.first) : gp.g.EdgeEnd(pos.first);
GraphComponent<Graph> component = omnigraph::VertexNeighborhood<Graph>(gp.g, v);
visualization::visualization_utils::WriteComponent<Graph>(component, file, colorer, labeler);
}
inline
optional<RtSeq> FindCloseKP1mer(const conj_graph_pack &gp,
size_t genome_pos, size_t k) {
VERIFY(gp.genome.size() > 0);
VERIFY(genome_pos < gp.genome.size());
static const size_t magic_const = 200;
for (size_t diff = 0; diff < magic_const; diff++) {
for (int dir = -1; dir <= 1; dir += 2) {
size_t pos = (gp.genome.size() - k + genome_pos + dir * diff) % (gp.genome.size() - k);
RtSeq kp1mer = gp.kmer_mapper.Substitute(
RtSeq (k + 1, gp.genome.GetSequence(), pos));
if (gp.index.contains(kp1mer))
return optional<RtSeq>(kp1mer);
}
}
return boost::none;
}
inline
void PrepareForDrawing(conj_graph_pack &gp) {
gp.EnsureDebugInfo();
CollectContigPositions(gp);
}
struct detail_info_printer {
detail_info_printer(conj_graph_pack &gp,
const visualization::graph_labeler::GraphLabeler<Graph>& labeler,
const string& folder)
: gp_(gp),
labeler_(labeler),
folder_(folder) {
}
void operator() (config::info_printer_pos pos,
const string& folder_suffix = "") {
string pos_name = ModeName(pos, config::InfoPrinterPosNames());
ProduceDetailedInfo(pos_name + folder_suffix, pos);
}
private:
template<typename T>
std::string ToString(const T& t, size_t length) {
std::ostringstream ss;
ss << t;
std::string result = ss.str();
while (result.size() < length)
result = "0" + result;
return result;
}
void ProduceDetailedInfo(const string &pos_name,
config::info_printer_pos pos) {
using namespace visualization;
static size_t call_cnt = 0;
auto it = cfg::get().info_printers.find(pos);
VERIFY(it != cfg::get().info_printers.end());
const config::debruijn_config::info_printer & config = it->second;
if (config.basic_stats) {
VertexEdgeStat<conj_graph_pack::graph_t> stats(gp_.g);
INFO("Number of vertices : " << stats.vertices() << ", number of edges : "
<< stats.edges() << ", sum length of edges : " << stats.edge_length());
}
if (config.save_graph_pack) {
string saves_folder = fs::append_path(fs::append_path(folder_, "saves/"),
ToString(call_cnt++, 2) + "_" + pos_name + "/");
fs::make_dirs(saves_folder);
graphio::ConjugateDataPrinter<conj_graph_pack::graph_t> printer(gp_.g);
graphio::PrintGraphPack(saves_folder + "graph_pack", printer, gp_);
//TODO: separate
graphio::PrintClusteredIndices(saves_folder + "graph_pack", printer, gp_.clustered_indices);
}
if (config.save_all) {
string saves_folder = fs::append_path(fs::append_path(folder_, "saves/"),
ToString(call_cnt++, 2) + "_" + pos_name);
fs::make_dirs(saves_folder);
string p = saves_folder + "/saves";
INFO("Saving current state to " << p);
debruijn_graph::graphio::PrintAll(p, gp_);
debruijn_graph::config::write_lib_data(p);
}
if (config.save_full_graph) {
string saves_folder = fs::append_path(fs::append_path(folder_, "saves/"),
ToString(call_cnt++, 2) + "_" + pos_name + "/");
fs::make_dirs(saves_folder);
graphio::ConjugateDataPrinter<conj_graph_pack::graph_t> printer(gp_.g);
graphio::PrintBasicGraph(saves_folder + "graph", printer);
}
if (config.lib_info) {
string saves_folder = fs::append_path(fs::append_path(folder_, "saves/"),
ToString(call_cnt++, 2) + "_" + pos_name + "/");
fs::make_dirs(saves_folder);
config::write_lib_data(saves_folder + "lib_info");
}
if (config.extended_stats) {
VERIFY(cfg::get().developer_mode);
CountStats(gp_);
}
if (!(config.write_error_loc ||
config.write_full_graph ||
config.write_full_nc_graph ||
config.write_components ||
!config.components_for_kmer.empty() ||
config.write_components_along_genome ||
config.write_components_along_contigs ||
!config.components_for_genome_pos.empty())) {
return;
}
VERIFY(cfg::get().developer_mode);
string pics_folder = fs::append_path(fs::append_path(folder_, "pictures/"),
ToString(call_cnt++, 2) + "_" + pos_name + "/");
fs::make_dirs(pics_folder);
PrepareForDrawing(gp_);
auto path1 = FindGenomeMappingPath(gp_.genome.GetSequence(), gp_.g, gp_.index,
gp_.kmer_mapper).path();
auto colorer = DefaultColorer(gp_);
if (config.write_error_loc) {
make_dir(pics_folder + "error_loc/");
WriteErrorLoc(gp_.g, pics_folder + "error_loc/", colorer, labeler_);
}
if (config.write_full_graph) {
visualization_utils::WriteComponent(GraphComponent<Graph>::WholeGraph(gp_.g),
pics_folder + "full_graph.dot", colorer, labeler_);
}
if (config.write_full_nc_graph) {
visualization_utils::WriteSimpleComponent(GraphComponent<Graph>::WholeGraph(gp_.g),
pics_folder + "nc_full_graph.dot", colorer, labeler_);
}
if (config.write_components) {
make_dir(pics_folder + "components/");
visualization_utils::WriteComponents(gp_.g, pics_folder + "components/",
omnigraph::ReliableSplitter<Graph>(gp_.g), colorer, labeler_);
}
if (!config.components_for_kmer.empty()) {
string kmer_folder = fs::append_path(pics_folder, "kmer_loc/");
make_dir(kmer_folder);
auto kmer = RtSeq(gp_.k_value + 1, config.components_for_kmer.substr(0, gp_.k_value + 1).c_str());
string file_name = fs::append_path(kmer_folder, pos_name + ".dot");
WriteKmerComponent(gp_, kmer, file_name, colorer, labeler_);
}
if (config.write_components_along_genome) {
make_dir(pics_folder + "along_genome/");
visualization_utils::WriteComponentsAlongPath
(gp_.g, path1.sequence(), pics_folder + "along_genome/", colorer, labeler_);
}
if (config.write_components_along_contigs) {
make_dir(pics_folder + "along_contigs/");
BasicSequenceMapper<Graph, Index> mapper(gp_.g, gp_.index, gp_.kmer_mapper);
WriteGraphComponentsAlongContigs(gp_.g, mapper, pics_folder + "along_contigs/", colorer, labeler_);
}
if (!config.components_for_genome_pos.empty()) {
string pos_loc_folder = fs::append_path(pics_folder, "pos_loc/");
make_dir(pos_loc_folder);
vector<string> positions;
boost::split(positions, config.components_for_genome_pos,
boost::is_any_of(" ,"), boost::token_compress_on);
for (auto it = positions.begin(); it != positions.end(); ++it) {
boost::optional<RtSeq> close_kp1mer = FindCloseKP1mer(gp_,
std::stoi(*it), gp_.k_value);
if (close_kp1mer) {
string locality_folder = fs::append_path(pos_loc_folder, *it + "/");
make_dir(locality_folder);
WriteKmerComponent(gp_, *close_kp1mer, fs::append_path(locality_folder, pos_name + ".dot"), colorer, labeler_);
} else {
WARN(
"Failed to find genome kp1mer close to the one at position "
<< *it << " in the graph. Which is " << RtSeq (gp_.k_value + 1, gp_.genome.GetSequence(), std::stoi(*it)));
}
}
}
}
conj_graph_pack& gp_;
const visualization::graph_labeler::GraphLabeler<Graph>& labeler_;
string folder_;
};
inline
std::string ConstructComponentName(std::string file_name, size_t cnt) {
stringstream ss;
ss << cnt;
string res = file_name;
res.insert(res.length(), ss.str());
return res;
}
template<class Graph>
double AvgCoverage(const Graph& g,
const std::vector<typename Graph::EdgeId>& edges) {
double total_cov = 0.;
size_t total_length = 0;
for (auto it = edges.begin(); it != edges.end(); ++it) {
total_cov += g.coverage(*it) * (double) g.length(*it);
total_length += g.length(*it);
}
return total_cov / (double) total_length;
}
template<class Graph>
size_t Nx(Graph &g, double percent) {
size_t sum_edge_length = 0;
vector<size_t> lengths;
for (auto iterator = g.ConstEdgeBegin(); !iterator.IsEnd(); ++iterator) {
lengths.push_back(g.length(*iterator));
sum_edge_length += g.length(*iterator);
}
sort(lengths.begin(), lengths.end());
double len_perc = (1.0 - percent * 0.01) * (double) (sum_edge_length);
for (size_t i = 0; i < lengths.size(); i++) {
if (lengths[i] >= len_perc)
return lengths[i];
else
len_perc -= (double) lengths[i];
}
return 0;
}
}
}
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