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/*===========================================================================
*
* PUBLIC DOMAIN NOTICE
* National Center for Biotechnology Information
*
* This software/database is a "United States Government Work" under the
* terms of the United States Copyright Act. It was written as part of
* the author's official duties as a United States Government employee and
* thus cannot be copyrighted. This software/database is freely available
* to the public for use. The National Library of Medicine and the U.S.
* Government have not placed any restriction on its use or reproduction.
*
* Although all reasonable efforts have been taken to ensure the accuracy
* and reliability of the software and data, the NLM and the U.S.
* Government do not and cannot warrant the performance or results that
* may be obtained by using this software or data. The NLM and the U.S.
* Government disclaim all warranties, express or implied, including
* warranties of performance, merchantability or fitness for any particular
* purpose.
*
* Please cite the author in any work or product based on this material.
*
* ===========================================================================
*
*/
typedef struct BamReaderFile BamReaderFile;
typedef struct BamRecord BamRecord;
typedef struct BamSequence BamSequence;
typedef struct BamAlignment BamAlignment;
typedef struct BamCGData BamCGData;
typedef struct BamReferenceInfo BamReferenceInfo;
#define READERFILE_IMPL BamReaderFile
#define RECORD_IMPL BamRecord
#define SEQUENCE_IMPL BamSequence
#define ALIGNMENT_IMPL BamAlignment
#define CGDATA_IMPL BamCGData
#define REFERENCEINFO_IMPL BamReferenceInfo
#include "bam-reader.h"
#include <stdlib.h>
#include <string.h>
#include <assert.h>
#include <loader/common-reader-priv.h>
struct BamSequence
{
Sequence dadSeq;
Alignment dadAlign;
CGData dadCG;
KRefcount refcount;
const BAMAlignment* bam;
uint16_t flags;
};
struct BamRecord
{
Record dad;
BamSequence seq;
Rejected* rej;
};
/*TODO: remove when done refactoring */
const BAMAlignment *ToBamAlignment(const Record* record)
{
assert(record);
return ((const BamRecord*)record)->seq.bam;
}
static rc_t BamSequenceInit(BamSequence* self);
/*--------------------------------------------------------------------------
* BamRecord
*/
static rc_t BamRecordAddRef ( const BamRecord* self );
static rc_t BamRecordRelease ( const BamRecord* self );
static rc_t BamRecordGetSequence ( const BamRecord* self, const Sequence** result);
static rc_t BamRecordGetAlignment ( const BamRecord* self, const Alignment** result);
static rc_t BamRecordGetRejected ( const BamRecord* self, const Rejected** result);
static Record_vt_v1 BamRecord_vt =
{
1, 0,
/* start minor version == 0 */
BamRecordAddRef,
BamRecordRelease,
BamRecordGetSequence,
BamRecordGetAlignment,
BamRecordGetRejected,
/* end minor version == 0 */
};
static rc_t CC BamRecordInit ( BamRecord* self )
{
assert(self);
self->dad.vt.v1 = & BamRecord_vt;
self->rej = 0;
return BamSequenceInit(& self->seq);
}
static rc_t BamRecordWhack( const BamRecord* self )
{
rc_t rc = 0;
assert(self);
rc = BAMAlignmentRelease(self->seq.bam);
if (rc != 0)
RejectedRelease(self->rej);
else
rc = RejectedRelease(self->rej);
free ( (BamRecord*) self );
return rc;
}
static rc_t BamRecordAddRef( const BamRecord* self )
{
assert(self);
KRefcountAdd( & self->seq.refcount, "BamRecord" );
/* TODO: handle rc from KRefcountAdd */
return 0;
}
static rc_t BamRecordRelease( const BamRecord* cself )
{
if ( cself != NULL )
{
BamRecord *self = ( BamRecord* ) cself;
switch ( KRefcountDrop ( & self ->seq.refcount, "BamRecord" ) )
{
case krefWhack:
BamRecordWhack( self );
break;
default:
/* TODO: handle other values */
break;
}
}
return 0;
}
static rc_t BamRecordGetSequence( const BamRecord* self, const Sequence** result )
{
rc_t rc = 0;
assert(result);
rc = BamRecordAddRef(self);
if (rc == 0)
*result = (const Sequence*) & self->seq;
else
*result = 0;
return rc;
}
static rc_t BamRecordGetAlignment( const BamRecord* self, const Alignment** result)
{
rc_t rc = 0;
assert(result);
/* do not allow access is flagged as unmapped*/
if ((self->seq.flags & BAMFlags_SelfIsUnmapped) != 0)
*result = 0;
else
{
rc = BamRecordAddRef(self);
if (rc == 0)
*result = (const Alignment*) & self->seq.dadAlign;
else
*result = 0;
}
return rc;
}
static rc_t BamRecordGetRejected( const BamRecord* self, const Rejected** result)
{
rc_t rc = 0;
assert(result);
*result = 0;
if (self->rej != 0)
{
rc = RejectedAddRef(self->rej);
if (rc == 0)
*result = self->rej;
}
return rc;
}
/*--------------------------------------------------------------------------
* BamSequence forwards
*/
static rc_t BamSequenceAddRef ( const BamSequence* self );
static rc_t BamSequenceRelease ( const BamSequence* self );
static rc_t BamSequenceGetReadLength ( const BamSequence *self, uint32_t *length );
static rc_t BamSequenceGetRead ( const BamSequence *self, char *sequence );
static rc_t BamSequenceGetRead2 ( const BamSequence *self, char *sequence, uint32_t start, uint32_t stop );
static rc_t BamSequenceGetQuality ( const BamSequence *self, const int8_t **quality, uint8_t *offset, int *qualType );
static rc_t BamSequenceGetSpotGroup ( const BamSequence *self, const char **name, size_t *length );
static rc_t BamSequenceGetSpotName ( const BamSequence *self, const char **name, size_t *length );
static bool BamSequenceIsColorSpace ( const BamSequence *self );
static rc_t BamSequenceGetCSKey ( const BamSequence *self, char cskey[1] );
static rc_t BamSequenceGetCSReadLength ( const BamSequence *self, uint32_t *length );
static rc_t BamSequenceGetCSRead ( const BamSequence *self, char *sequence );
static rc_t BamSequenceGetCSQuality ( const BamSequence *self, const int8_t **quality, uint8_t *offset, int *qualType );
static bool BamSequenceRecordWasPaired ( const BamSequence *self );
static int BamSequenceRecordOrientationSelf ( const BamSequence *self );
static int BamSequenceRecordOrientationMate ( const BamSequence *self );
static bool BamSequenceRecordIsFirst ( const BamSequence *self );
static bool BamSequenceRecordIsSecond ( const BamSequence *self );
static bool BamSequenceRecordIsDuplicate ( const BamSequence *self );
static bool BamSequenceIsLowQuality ( const BamSequence *self );
static rc_t BamSequenceGetTI ( const BamSequence *self, uint64_t *ti );
/*--------------------------------------------------------------------------
* BamCGData
*/
static rc_t BamCGDataAddRef ( const BamCGData* self );
static rc_t BamCGDataRelease ( const BamCGData* self );
static rc_t BamCGDataGetSeqQual ( const BamCGData* self, char sequence[/* 35 */], uint8_t quality[/* 35 */] );
static rc_t BamCGDataGetCigar ( const BamCGData* self, uint32_t *cigar, uint32_t cig_max, uint32_t *cig_act );
static rc_t BamCGDataGetAlignGroup ( const BamCGData* self, char buffer[], size_t max_size, size_t *act_size );
/* CGGetAlignGroup
*/
rc_t CC CGDataGetAlignGroup ( const CGData* self,
char buffer[],
size_t max_size,
size_t *act_size );
static CGData_vt_v1 BamCGData_vt = {
1, 0,
/* start minor version == 0 */
BamCGDataAddRef,
BamCGDataRelease,
BamCGDataGetSeqQual,
BamCGDataGetCigar,
BamCGDataGetAlignGroup
/* end minor version == 0 */
};
static const BamSequence*
BamCGDataToSequence(const BamCGData* cg)
{
return ( const BamSequence * ) ( (uint8_t*)cg- offsetof ( BamSequence, dadCG) );
}
static rc_t BamCGDataAddRef ( const BamCGData* self )
{
return BamSequenceAddRef(BamCGDataToSequence(self));
}
static rc_t BamCGDataRelease ( const BamCGData* self )
{
return BamSequenceRelease(BamCGDataToSequence(self));
}
static rc_t BamCGDataGetSeqQual ( const BamCGData* self, char sequence[/* 35 */], uint8_t quality[/* 35 */] )
{
return BAMAlignmentGetCGSeqQual(BamCGDataToSequence(self)->bam, sequence, quality);
}
static rc_t BamCGDataGetCigar ( const BamCGData* self, uint32_t *cigar, uint32_t cig_max, uint32_t *cig_act )
{
return BAMAlignmentGetCGCigar(BamCGDataToSequence(self)->bam, cigar, cig_max, cig_act);
}
static rc_t BamCGDataGetAlignGroup ( const BamCGData* self, char buffer[], size_t max_size, size_t *act_size )
{
return BAMAlignmentGetCGAlignGroup(BamCGDataToSequence(self)->bam, buffer, max_size, act_size);
}
/*--------------------------------------------------------------------------
* BamAlignment
*/
static rc_t BamAlignmentAddRef ( const BamAlignment* self );
static rc_t BamAlignmentRelease ( const BamAlignment* self );
static rc_t BamAlignmentGetRefSeqId ( const BamAlignment *self, int32_t *refSeqId );
static rc_t BamAlignmentGetMateRefSeqId ( const BamAlignment *self, int32_t *refSeqId );
static rc_t BamAlignmentGetPosition ( const BamAlignment *self, int64_t *pos );
static rc_t BamAlignmentGetMatePosition ( const BamAlignment *self, int64_t *pos );
static rc_t BamAlignmentGetMapQuality ( const BamAlignment *self, uint8_t *qual );
static rc_t BamAlignmentGetAlignmentDetail ( const BamAlignment *self, AlignmentDetail *rslt, uint32_t count, uint32_t *actual, int32_t *firstMatch, int32_t *lastMatch );
static rc_t BamAlignmentGetAlignOpCount ( const BamAlignment *self, uint32_t *n );
static rc_t BamAlignmentGetInsertSize ( const BamAlignment *self, int64_t *size );
static rc_t BamAlignmentGetCGData ( const BamAlignment *self, const CGData** cg);
static rc_t BamAlignmentGetBAMCigar ( const BamAlignment *self, uint32_t const **rslt, uint32_t *length );
static bool BamAlignmentIsSecondary ( const BamAlignment *self );
static Alignment_vt_v1 BamAlignment_vt = {
1, 0,
/* start minor version == 0 */
BamAlignmentAddRef,
BamAlignmentRelease,
BamAlignmentGetRefSeqId,
BamAlignmentGetMateRefSeqId,
BamAlignmentGetPosition,
BamAlignmentGetMatePosition,
BamAlignmentGetMapQuality,
BamAlignmentGetAlignmentDetail,
BamAlignmentGetAlignOpCount,
BamAlignmentGetInsertSize,
BamAlignmentGetCGData,
BamAlignmentGetBAMCigar,
BamAlignmentIsSecondary,
/* end minor version == 0 */
};
static const BamSequence*
BamAlignmentToSequence(const BamAlignment* align)
{
return ( const BamSequence * ) ( (uint8_t*)align - offsetof ( BamSequence, dadAlign ) );
}
static rc_t BamAlignmentAddRef ( const BamAlignment* self )
{
return BamSequenceAddRef(BamAlignmentToSequence(self));
}
static rc_t BamAlignmentRelease ( const BamAlignment* self )
{
return BamSequenceRelease(BamAlignmentToSequence(self));
}
static rc_t BamAlignmentGetRefSeqId ( const BamAlignment *self, int32_t *refSeqId )
{
return BAMAlignmentGetRefSeqId(BamAlignmentToSequence(self)->bam, refSeqId);
}
static rc_t BamAlignmentGetMateRefSeqId ( const BamAlignment *self, int32_t *refSeqId )
{
return BAMAlignmentGetMateRefSeqId(BamAlignmentToSequence(self)->bam, refSeqId);
}
static rc_t BamAlignmentGetPosition ( const BamAlignment *self, int64_t *pos )
{
return BAMAlignmentGetPosition(BamAlignmentToSequence(self)->bam, pos);
}
static rc_t BamAlignmentGetMatePosition ( const BamAlignment *self, int64_t *pos )
{
return BAMAlignmentGetMatePosition(BamAlignmentToSequence(self)->bam, pos);
}
static rc_t BamAlignmentGetMapQuality ( const BamAlignment *self, uint8_t *qual )
{
return BAMAlignmentGetMapQuality(BamAlignmentToSequence(self)->bam, qual);
}
static rc_t BamAlignmentGetAlignmentDetail ( const BamAlignment *self, AlignmentDetail *rslt, uint32_t count, uint32_t *actual, int32_t *firstMatch, int32_t *lastMatch )
{
return BAMAlignmentGetAlignmentDetail ( BamAlignmentToSequence(self)->bam, (BAMAlignmentDetail*)rslt, count, actual, firstMatch, lastMatch );
}
static rc_t BamAlignmentGetAlignOpCount ( const BamAlignment *self, uint32_t *n )
{
return BAMAlignmentGetCigarCount ( BamAlignmentToSequence(self)->bam, n );
}
static rc_t BamAlignmentGetInsertSize ( const BamAlignment *self, int64_t *size )
{
return BAMAlignmentGetInsertSize ( BamAlignmentToSequence(self)->bam, size );
}
static rc_t BamAlignmentGetCGData ( const BamAlignment *self, const CGData** cg)
{
rc_t rc = 0;
if (BAMAlignmentHasCGData(BamAlignmentToSequence(self)->bam))
{
rc_t rc = BamAlignmentAddRef(self);
if (rc == 0)
*cg = (const CGData*) & BamAlignmentToSequence(self)->dadCG;
else
*cg = 0;
}
else
*cg = 0;
return rc;
}
static rc_t BamAlignmentGetBAMCigar ( const BamAlignment *self, uint32_t const **rslt, uint32_t *length )
{
return BAMAlignmentGetRawCigar(BamAlignmentToSequence(self)->bam, rslt, length);
}
static bool BamAlignmentIsSecondary( const BamAlignment *self )
{
return (BamAlignmentToSequence(self)->flags & BAMFlags_IsNotPrimary) != 0;
}
/*--------------------------------------------------------------------------
* BamSequence
*/
static Sequence_vt_v1 BamSequence_vt =
{
1, 0,
/* start minor version == 0 */
BamSequenceAddRef,
BamSequenceRelease,
BamSequenceGetReadLength,
BamSequenceGetRead,
BamSequenceGetRead2,
BamSequenceGetQuality,
BamSequenceGetSpotGroup,
BamSequenceGetSpotName,
BamSequenceIsColorSpace,
BamSequenceGetCSKey,
BamSequenceGetCSReadLength,
BamSequenceGetCSRead,
BamSequenceGetCSQuality,
BamSequenceRecordWasPaired,
BamSequenceRecordOrientationSelf,
BamSequenceRecordOrientationMate,
BamSequenceRecordIsFirst,
BamSequenceRecordIsSecond,
BamSequenceRecordIsDuplicate,
BamSequenceIsLowQuality,
BamSequenceGetTI,
/* end minor version == 0 */
};
static
rc_t
BamSequenceInit(BamSequence* self)
{
self->dadSeq.vt.v1 = & BamSequence_vt;
self->dadAlign.vt.v1 = & BamAlignment_vt;
self->dadCG.vt.v1 = & BamCGData_vt;
KRefcountInit ( & self -> refcount, 1, "BamSequence", "BamSequenceInit", "");
self->bam= 0;
self->flags = 0;
return 0;
}
static const BamRecord*
BamSequenceToRecord(const BamSequence* seq)
{
return ( const BamRecord * ) ( (uint8_t*)seq - offsetof ( BamRecord, seq ) );
}
static rc_t BamSequenceAddRef( const BamSequence* self )
{
switch (KRefcountAdd( & self->refcount, "BamSequence" ))
{
case krefLimit:
return RC ( RC_MODULE, rcData, rcAttaching, rcRange, rcExcessive );
case krefNegative:
return RC ( RC_MODULE, rcData, rcAttaching, rcRefcount, rcInvalid );
}
return 0;
}
static rc_t BamSequenceRelease( const BamSequence* self )
{
if ( self != NULL )
{
switch ( KRefcountDrop ( & self -> refcount, "BamSequence" ) )
{
case krefWhack:
return BamRecordWhack ( BamSequenceToRecord(self) );
case krefNegative:
return RC ( RC_MODULE, rcData, rcDestroying, rcSelf, rcDestroyed );
}
}
return 0;
}
static rc_t BamSequenceGetReadLength ( const BamSequence *self, uint32_t *length )
{
assert(self);
assert(self->bam);
return BAMAlignmentGetReadLength(self->bam, length);
}
static rc_t BamSequenceGetRead ( const BamSequence *self, char *sequence )
{
assert(self);
assert(self->bam);
assert(sequence);
return BAMAlignmentGetSequence(self->bam, sequence);
}
static rc_t BamSequenceGetRead2 ( const BamSequence *self, char *sequence, uint32_t start, uint32_t stop )
{
assert(self);
assert(self->bam);
assert(sequence);
return BAMAlignmentGetSequence2(self->bam, sequence, start, stop);
}
static rc_t BamSequenceGetQuality ( const BamSequence *self, const int8_t **quality, uint8_t *offset, int *qualType )
{
assert(self);
assert(self->bam);
assert(quality);
assert(offset);
assert(qualType);
{
rc_t rc = BAMAlignmentGetQuality2(self->bam, (const uint8_t **)quality, offset);
if (rc != 0)
{
*quality = NULL;
*offset = 0;
*qualType = 0;
}
else
*qualType = QT_Phred;
return rc;
}
}
static rc_t BamSequenceGetSpotGroup ( const BamSequence *self, const char **name, size_t *length )
{
assert(self);
assert(self->bam);
assert(name);
{
rc_t rc = BAMAlignmentGetReadGroupName ( self->bam, name );
if (rc != 0)
{
*name = NULL;
*length = 0;
}
else
*length = string_size(*name);
return rc;
}
}
static rc_t BamSequenceGetSpotName ( const BamSequence *self, const char **name, size_t *length )
{
assert(self);
assert(self->bam);
assert(name);
{
rc_t rc = BAMAlignmentGetReadName ( self->bam, name );
if (rc != 0)
{
*name = NULL;
*length = 0;
}
else
*length = string_size(*name);
return rc;
}
}
static bool BamSequenceIsColorSpace ( const BamSequence *self )
{
assert(self);
assert(self->bam);
return BAMAlignmentHasColorSpace(self->bam);
}
static rc_t BamSequenceGetCSKey ( const BamSequence *self, char cskey[1] )
{
assert(self);
assert(self->bam);
return BAMAlignmentGetCSKey(self->bam, cskey);
}
static rc_t BamSequenceGetCSReadLength ( const BamSequence *self, uint32_t *length )
{
assert(self);
assert(self->bam);
assert(length);
return BAMAlignmentGetCSSeqLen(self->bam, length);
}
static rc_t BamSequenceGetCSRead ( const BamSequence *self, char *sequence )
{
assert(self);
assert(self->bam);
assert(sequence);
{
uint32_t length;
rc_t rc = BAMAlignmentGetCSSeqLen(self->bam, &length);
return rc ? rc : BAMAlignmentGetCSSequence(self->bam, sequence, length);
}
}
static rc_t BamSequenceGetCSQuality ( const BamSequence *self, const int8_t **quality, uint8_t *offset, int *qualType )
{
assert(self);
assert(self->bam);
assert(quality);
assert(offset);
assert(qualType);
{
rc_t rc = BAMAlignmentGetCSQuality(self->bam, (const uint8_t **)quality, offset);
if (rc != 0)
{
*quality = NULL;
*offset = 0;
*qualType = 0;
}
else
*qualType = QT_Phred;
return rc;
}
}
static bool BamSequenceRecordWasPaired ( const BamSequence *self )
{
return self->flags & BAMFlags_WasPaired;
}
static int BamSequenceRecordOrientationSelf ( const BamSequence *self )
{
return (self->flags & BAMFlags_SelfIsReverse) ? ReadOrientationReverse : ReadOrientationForward;
}
static int BamSequenceRecordOrientationMate ( const BamSequence *self )
{
return (self->flags & BAMFlags_MateIsReverse) ? ReadOrientationReverse : ReadOrientationForward;;
}
static bool BamSequenceRecordIsFirst ( const BamSequence *self )
{
return self->flags & BAMFlags_IsFirst;
}
static bool BamSequenceRecordIsSecond ( const BamSequence *self )
{
return self->flags & BAMFlags_IsSecond;
}
static bool BamSequenceRecordIsDuplicate ( const BamSequence *self )
{
return self->flags & BAMFlags_IsDuplicate;
}
static bool BamSequenceIsLowQuality ( const BamSequence *self )
{
return self->flags & BAMFlags_IsLowQuality;
}
static rc_t BamSequenceGetTI ( const BamSequence *self, uint64_t *ti )
{
return BAMAlignmentGetTI(self->bam, ti);
}
/*--------------------------------------------------------------------------
* BamReferenceInfo forwards
*/
static rc_t BamReferenceAddRef ( const BamReferenceInfo* self );
static rc_t BamReferenceRelease ( const BamReferenceInfo* self );
static rc_t BamReferenceInfoGetRefSeqCount ( const BamReferenceInfo* self, uint32_t* count );
static rc_t BamReferenceInfoGetRefSeq ( const BamReferenceInfo* self, uint32_t n, ReferenceSequence* result );
static rc_t BamReferenceInfoGetReadGroupCount ( const BamReferenceInfo* self, uint32_t* count );
static rc_t BamReferenceInfoGetReadGroup ( const BamReferenceInfo* self, unsigned n, ReadGroup* result );
static rc_t BamReferenceInfoGetReadGroupByName ( const BamReferenceInfo* self, const char* name, ReadGroup* result );
static ReferenceInfo_vt_v1 BamReferenceInfo_vt =
{
1, 0,
/* start minor version == 0 */
BamReferenceAddRef,
BamReferenceRelease,
BamReferenceInfoGetRefSeqCount,
BamReferenceInfoGetRefSeq,
BamReferenceInfoGetReadGroupCount,
BamReferenceInfoGetReadGroup,
BamReferenceInfoGetReadGroupByName,
/* end minor version == 0 */
};
/*--------------------------------------------------------------------------
* BamReaderFile
*/
static rc_t BamReaderFileWhack ( BamReaderFile* self );
static rc_t BamReaderFileGetRecord ( const BamReaderFile *self, const Record** result );
static float BamReaderFileGetProportionalPosition ( const BamReaderFile *self );
static rc_t BamReaderFileGetReferenceInfo ( const BamReaderFile *self, const ReferenceInfo** result );
static ReaderFile_vt_v1 BamReaderFile_vt =
{
1, 0,
/* start minor version == 0 */
BamReaderFileWhack,
BamReaderFileGetRecord,
BamReaderFileGetProportionalPosition,
BamReaderFileGetReferenceInfo,
/* end minor version == 0 */
};
struct BamReaderFile
{
ReaderFile dad;
ReferenceInfo refInfo;
const BAMFile* reader;
};
const BAMFile* ToBam(const ReaderFile* reader)
{
assert(reader);
return ((const BamReaderFile*)reader)->reader;
}
float BamReaderFileGetProportionalPosition ( const BamReaderFile *f )
{
BamReaderFile* self = (BamReaderFile*) f;
return BAMFileGetProportionalPosition(self->reader);
}
rc_t BamReaderFileGetReferenceInfo ( const BamReaderFile *self, const ReferenceInfo** result )
{
*result = & self->refInfo;
return ReaderFileAddRef( & self->dad );
}
rc_t BamReaderFileWhack( BamReaderFile* f )
{
BamReaderFile* self = (BamReaderFile*) f;
BAMFileRelease(self->reader);
free ( (void*)self->dad.pathname );
free ( self );
return 0;
}
rc_t BamReaderFileGetRecord ( const BamReaderFile *f, const Record** result )
{
rc_t rc;
BamReaderFile* self = (BamReaderFile*) f;
BamRecord* record = (BamRecord*)malloc(sizeof(BamRecord));
if (record == NULL)
{
rc = RC ( RC_MODULE, rcData, rcAllocating, rcMemory, rcExhausted );
return 0;
}
rc = BamRecordInit(record);
if (rc != 0)
{
free(record);
return rc;
}
rc = BAMFileRead2(self->reader, &record->seq.bam);
if (rc)
{
if (GetRCModule(rc) == rcAlign && GetRCObject(rc) == rcRow && GetRCState(rc) == rcNotFound)
{ /* end of input */
rc = 0;
}
*result = 0;
BamRecordRelease(record);
}
else
{
*result = (const Record*)record;
BAMAlignmentGetFlags(record->seq.bam, &record->seq.flags);
}
return rc;
}
rc_t CC BamReaderFileMake( const ReaderFile **reader, char const headerText[], char const path[])
{
rc_t rc;
BamReaderFile* self = (BamReaderFile*) malloc ( sizeof * self );
if ( self == NULL )
{
rc = RC ( RC_MODULE, rcFileFormat, rcAllocating, rcMemory, rcExhausted );
*reader = 0;
}
else
{
rc = ReaderFileInit ( self );
self->dad.vt.v1 = & BamReaderFile_vt;
self->refInfo.vt.v1 = & BamReferenceInfo_vt;
self->dad.pathname = string_dup(path, strlen(path)+1);
if ( self->dad.pathname == NULL )
{
rc = RC ( RC_MODULE, rcFileFormat, rcAllocating, rcMemory, rcExhausted );
}
rc = BAMFileMakeWithHeader ( &self->reader, headerText, "%s", self->dad.pathname );
if (rc != 0)
{
BamReaderFileWhack( self );
*reader = 0;
}
else
{
*reader = (const ReaderFile *) self;
}
}
return rc;
}
/*--------------------------------------------------------------------------
* BamReferenceInfo
*/
static const BamReaderFile*
BamRefInfoToReader(const BamReferenceInfo* ref)
{
return ( const BamReaderFile * ) ( (uint8_t*)ref - offsetof ( BamReaderFile, refInfo ) );
}
rc_t BamReferenceAddRef ( const BamReferenceInfo* self )
{
return ReaderFileAddRef( & BamRefInfoToReader(self)->dad );
}
rc_t BamReferenceRelease ( const BamReferenceInfo* self )
{
return ReaderFileRelease ( & BamRefInfoToReader(self)->dad );
}
rc_t BamReferenceInfoGetRefSeqCount ( const BamReferenceInfo* self, uint32_t* count )
{
return BAMFileGetRefSeqCount( BamRefInfoToReader(self)->reader, count );
}
rc_t BamReferenceInfoGetRefSeq ( const BamReferenceInfo *self, uint32_t n, ReferenceSequence *result )
{
const BAMRefSeq *refSeq;
rc_t rc = BAMFileGetRefSeq( BamRefInfoToReader(self)->reader, n, &refSeq );
if (rc != 0 || refSeq == NULL)
{
return RC ( RC_MODULE, rcHeader, rcAccessing, rcParam, rcOutofrange );
}
result->length = refSeq->length;
result->name = refSeq->name;
result->checksum = refSeq->checksum;
return 0;
}
rc_t BamReferenceInfoGetReadGroupCount ( const BamReferenceInfo *self, uint32_t *count )
{
return BAMFileGetReadGroupCount ( BamRefInfoToReader(self)->reader, count );
}
rc_t BamReferenceInfoGetReadGroup ( const BamReferenceInfo *self, unsigned n, ReadGroup* result )
{
const BAMReadGroup *rg;
rc_t rc = BAMFileGetReadGroup( BamRefInfoToReader(self)->reader, n, &rg );
if (rc != 0 || rg == NULL)
{
return RC ( RC_MODULE, rcHeader, rcAccessing, rcParam, rcOutofrange );
}
result->name = rg->name;
result->platform = rg->platform;
return 0;
}
rc_t BamReferenceInfoGetReadGroupByName ( const BamReferenceInfo *self, const char *name, ReadGroup *result )
{
const BAMReadGroup *rg;
rc_t rc = BAMFileGetReadGroupByName( BamRefInfoToReader(self)->reader, name, &rg );
if (rc != 0 || rg == NULL)
{
return RC ( RC_MODULE, rcHeader, rcAccessing, rcParam, rcOutofrange );
}
result->name = rg->name;
result->platform = rg->platform;
return 0;
}
#ifdef TENTATIVE
/**************************** future parsing-on-a-thread code *****************************************/
#include "bam-reader.h"
#include <atomic32.h>
#include <stdlib.h>
#include <string.h>
#include <klib/rc.h>
#include <kproc/lock.h>
#include <kproc/cond.h>
#include <kproc/thread.h>
#define BUFFER_COUNT (3)
struct BAMReader
{
atomic32_t refcount;
const BAMFile* file;
KLock *lock;
KCondition *have_data;
KCondition *need_data;
KThread *th;
const BAMAlignment* que[BUFFER_COUNT];
unsigned volatile nque;
rc_t volatile rc;
bool eof;
};
static rc_t BAMReaderThreadMain(KThread const *const th, void *const vp);
#define END_OF_DATA RC(rcAlign, rcFile, rcReading, rcRow, rcNotFound)
rc_t BAMReaderMake( const BAMReader **result,
char const headerText[],
char const path[] )
{
rc_t rc;
BAMReader *self = malloc(sizeof(BAMReader));
if ( self == NULL )
{
*result = NULL;
return RC(rcAlign, rcFile, rcConstructing, rcMemory, rcExhausted);
}
else
{
atomic32_set( & self->refcount, 1 );
rc = BAMFileMakeWithHeader( & self->file, headerText, "%s", path);
if ( rc != 0 )
{
free(self);
*result = 0;
}
else
*result = self;
}
self->nque = 0;
self->rc = 0;
self->eof = false;
rc = KLockMake(&self->lock);
if (rc == 0)
{
rc = KConditionMake(&self->have_data);
if (rc == 0)
{
rc = KConditionMake(&self->need_data);
if (rc == 0)
{
rc = KThreadMake(&self->th, BAMReaderThreadMain, self);
if (rc == 0)
return 0;
KConditionRelease(self->need_data);
}
KConditionRelease(self->have_data);
}
KLockRelease(self->lock);
}
return rc;
}
static void BAMReaderWhack(BAMReader *const self)
{
KThreadCancel(self->th);
KThreadWait(self->th, NULL);
BAMFileRelease(self->file);
KConditionRelease(self->need_data);
KConditionRelease(self->have_data);
KLockRelease(self->lock);
KThreadRelease(self->th);
}
/* AddRef
* Release
*/
rc_t BAMReaderAddRef ( const BAMReader *self )
{
if (self != NULL)
atomic32_inc(&((BAMReader *)self)->refcount);
return 0;
}
rc_t BAMReaderRelease ( const BAMReader *cself )
{
BAMReader *self = (BAMReader *)cself;
if (cself != NULL)
{
if (atomic32_dec_and_test(&self->refcount))
{
BAMReaderWhack(self);
free(self);
}
}
return 0;
}
/* GetBAMFile
*/
const BAMFile* BAMReaderGetBAMFile ( const BAMReader *self )
{
if (self == NULL)
return NULL;
return self->file;
}
static rc_t BAMReaderThreadMain(KThread const *const th, void *const vp)
{
BAMReader *const self = (BAMReader *)vp;
rc_t rc = 0;
const BAMAlignment* rec;
KLockAcquire(self->lock);
do
{
while (self->nque == BUFFER_COUNT)
KConditionWait(self->need_data, self->lock);
{
rc = BAMFileRead( self->file, &rec);
if (rc == END_OF_DATA)
{
rec = NULL;
rc = 0;
}
else if (rc)
break;
self->que[self->nque] = rec;
++self->nque;
KConditionSignal(self->have_data);
}
}
while (rec);
self->rc = rc;
KLockUnlock(self->lock);
return 0;
}
/* Read
* read an aligment
*
* "result" [ OUT ] - return param for BAMAlignment object
* must be released with BAMAlignmentRelease
*
* returns RC(..., ..., ..., rcRow, rcNotFound) at end
*/
rc_t BAMReaderRead ( const BAMReader *cself, const BAMAlignment **result )
{
rc_t rc;
BAMReader *self = (BAMReader *)cself;
if (self == NULL)
return RC(rcAlign, rcFile, rcReading, rcParam, rcNull);
if (self->eof)
return RC(rcAlign, rcFile, rcReading, rcData, rcInsufficient);
KLockAcquire(self->lock);
if ((rc = self->rc) == 0)
{
while (self->nque == 0 && (rc = self->rc) == 0)
KConditionWait(self->have_data, self->lock);
if (rc == 0)
{
*result = self->que[0];
if (*result)
{
--self->nque;
memmove(&self->que[0], &self->que[1], self->nque * sizeof(self->que[0]));
KConditionSignal(self->need_data);
}
else
{
self->eof = true;
rc = END_OF_DATA;
}
}
}
KLockUnlock(self->lock);
return rc;
}
#endif
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