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#ifndef __CFASTQ_DEFLINE_MATCHER_HPP__
#define __CFASTQ_DEFLINE_MATCHER_HPP__
/**
* @file fastq_defline_matcher.hpp
* @brief Defline matcher classes
*
*/
#include "fastq_read.hpp"
#include "regexpr.hpp"
#include <absl/strings/match.h>
#include <insdc/sra.h>
using namespace std;
/* platform id
#define sra_platform_id_t "INSDC:SRA:platform_id"
typedef uint8_t INSDC_SRA_platform_id;
enum
{
SRA_PLATFORM_UNDEFINED = 0,
SRA_PLATFORM_454 = 1,
SRA_PLATFORM_ILLUMINA = 2,
SRA_PLATFORM_ABSOLID = 3,
SRA_PLATFORM_COMPLETE_GENOMICS = 4,
SRA_PLATFORM_HELICOS = 5,
SRA_PLATFORM_PACBIO_SMRT = 6,
SRA_PLATFORM_ION_TORRENT = 7,
SRA_PLATFORM_CAPILLARY = 8,
SRA_PLATFORM_OXFORD_NANOPORE = 9
};
*/
class CDefLineMatcher
/// Base class for all defline matchers
{
public:
/**
* @brief Construct a new CDefLineMatcher object
*
* @param defLineName defline description
* @param pattern defline regex pattern
*
* @throws runtime error on invalid pattern
*/
CDefLineMatcher(
const string& defLineName,
const string& pattern)
: mDefLineName( defLineName ),
re( pattern )
{
}
virtual ~CDefLineMatcher() {}
/**
* @brief Check if matcher recrogizes defline
*
* @param[in] defline string_view de fline to check
* @return true if defline matches
* @return false if defline does not match
*/
virtual bool Matches(const re2::StringPiece& defline)
{
return re.Matches(defline);
}
/**
* @brief retrun Defline description
*
* @return const string&
*/
const string& Defline() const { return mDefLineName;}
/**
* @brief Return matcher's pattern
*/
const string& GetPattern() const { return re.GetPattern();}
/**
* @brief Fill CFastqRead with the data from matched defline
*
* @param read
*/
virtual void GetMatch(CFastqRead& read) = 0;
/**
* @brief Return matcher's platform code
*
* @return uint8_t
*/
virtual uint8_t GetPlatform() const = 0;
protected:
string mDefLineName; ///< Defline description
CRegExprMatcher re; ///< regexpr matcher
string m_tmp_spot; ///< variable for spot name assembly
};
class CDefLineMatcher_NoMatch : public CDefLineMatcher
/// Matcher that matches nothing
{
public:
CDefLineMatcher_NoMatch():
CDefLineMatcher("NoMatch", "a^")
{
}
bool Matches(const re2::StringPiece& defline) override {return false;}
virtual void GetMatch(CFastqRead& read) override {}
virtual uint8_t GetPlatform() const override { return 0;}
protected:
};
class CDefLineMatcher_AllMatch : public CDefLineMatcher
/// Matcher that matches everything similar to defline
{
public:
CDefLineMatcher_AllMatch():
CDefLineMatcher("undefined", R"([@>+]([!-~]+)(\s+|$))")
{
}
//bool Matches(const string_view& defline) override { return true;}
virtual void GetMatch(CFastqRead& read) override {
read.SetSpot(re.GetMatch()[0]);
}
virtual uint8_t GetPlatform() const override { return 0;}
protected:
};
class CDefLineMatcherIlluminaNewDataGroup : public CDefLineMatcher
/// illuminaNewDataGroup matcher
{
public:
CDefLineMatcherIlluminaNewDataGroup() :
CDefLineMatcher(
"illuminaNewDataGroup",
R"(^[@>+]([!-~]+?)(\s+|[_|])([12345]|):([NY]):(\d+|O):?([!-~]*?)(\s+|$))")
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_ILLUMINA;
};
virtual void GetMatch(CFastqRead& read) override
{
read.SetSpot(re.GetMatch()[0]);
read.SetReadNum(re.GetMatch()[2]);
read.SetReadFilter(re.GetMatch()[3] == "Y" ? 1 : 0);
read.SetSpotGroup(re.GetMatch()[5]);
}
private:
};
static inline
void s_add_sep(string& s, re2::StringPiece& sep)
{
if (!sep.empty())
s.append(1, (sep[0] == '-') ? ':' : sep[0]);
}
class CDefLineMatcherIlluminaNewBase : public CDefLineMatcher
/// Base class for IlluminaNew matchers
{
public:
CDefLineMatcherIlluminaNewBase(
const string& displayName,
const string& pattern): CDefLineMatcher(displayName, pattern)
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_ILLUMINA;
};
virtual void GetMatch(CFastqRead& read) override
{
m_tmp_spot.clear();
if (!re.GetMatch()[0].empty()) {
m_tmp_spot.assign(re.GetMatch()[0].data(), re.GetMatch()[0].size());
s_add_sep(m_tmp_spot, re.GetMatch()[1]);
}
m_tmp_spot.append(re.GetMatch()[2].data(), re.GetMatch()[2].size()); //lane
s_add_sep(m_tmp_spot, re.GetMatch()[3]);
m_tmp_spot.append(re.GetMatch()[4].data(), re.GetMatch()[4].size()); //tile
s_add_sep(m_tmp_spot, re.GetMatch()[5]);
m_tmp_spot.append(re.GetMatch()[6].data(), re.GetMatch()[6].size()); //x
s_add_sep(m_tmp_spot, re.GetMatch()[7]);
m_tmp_spot.append(re.GetMatch()[8].data(), re.GetMatch()[8].size()); //y
read.MoveSpot(std::move(m_tmp_spot));
read.SetReadNum(re.GetMatch()[10]);
read.SetReadFilter(re.GetMatch()[11] == "Y" ? 1 : 0);
read.SetSpotGroup(re.GetMatch()[13]);
}
private:
};
static
bool s_is_number(const string_view& s)
{
return !s.empty() && find_if(s.begin(), s.end(), [](unsigned char c) { return !isdigit(c); }) == s.end();
}
class CDefLineMatcherIlluminaOldBase : public CDefLineMatcher
/// Base class for IlluminaNew matchers
{
CRegExprMatcher sub_re1; ///< additional regex to support numDiscards matching
CRegExprMatcher sub_re2; ///< additional regex to support numDiscards matching
CRegExprMatcher sub_re3; ///< additional regex to support numDiscards matching
CRegExprMatcher illuminaOldSuffix2; ///< additional regex for suffix matching
CRegExprMatcher illuminaOldSuffix; ///< additional regex for suffix matching
public:
CDefLineMatcherIlluminaOldBase(
const string& displayName,
const string& pattern): CDefLineMatcher(displayName, pattern),
sub_re1(R"(([!-~]*?)(:)(\d+)$)"),
sub_re2(R"(([!-~]*?)(:)(\d+)(:)(\d+)(\s+|$))"),
sub_re3(R"((\d+)(:)(\d+)(\s+|$))"),
illuminaOldSuffix2(R"((-?\d+\.\d+|-?\d+)([^\d\s.][!-~]+))"),
illuminaOldSuffix(R"((/[12345])([^\d\s][!-~]+))")
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_ILLUMINA;
};
//prefix, sep1, lane, sep2, tile, sep3, x, sep4, y, spotGroup, readNum, endSep
//0 1 2 3 4 5 6 7 8 9 10 11
virtual void GetMatch(CFastqRead& read) override
{
re2::StringPiece prefix{re.GetMatch()[0]};
auto& lane = re.GetMatch()[2];
auto& tile = re.GetMatch()[4];
auto& x = re.GetMatch()[6];
auto& y = re.GetMatch()[8];
auto& readNum = re.GetMatch()[10];
m_tmp_suffix = re2::StringPiece();
if (illuminaOldSuffix2.Matches(y)) {
y = illuminaOldSuffix2.GetMatch()[0];
auto& suffix = illuminaOldSuffix2.GetMatch()[1];
if (suffix.size() >= 3) {
if (absl::StartsWith(suffix, "/1") || absl::StartsWith(suffix, "/2"))
suffix.remove_prefix(2);
m_tmp_suffix = suffix;
}
} else if (!readNum.empty() && illuminaOldSuffix.Matches(readNum)) {
readNum = illuminaOldSuffix.GetMatch()[0];
if (illuminaOldSuffix.GetMatch()[1].size() >= 3)
m_tmp_suffix = illuminaOldSuffix.GetMatch()[1];
}
if (!m_tmp_suffix.empty())
read.SetSuffix(m_tmp_suffix);
int numDiscards = 0;
if (!prefix.empty()) {
numDiscards = countExtraNumbersInIllumina(prefix, re.GetMatch()[7], x, y);
if (numDiscards == 2 && x.find('.') != re2::StringPiece::npos) {
string new_suffix;
new_suffix.append(re.GetMatch()[5].data(), re.GetMatch()[5].size()); // sep3
new_suffix.append(x.data(), x.size()); //x
new_suffix.append(re.GetMatch()[7].data(), re.GetMatch()[7].size()); // sep4
new_suffix.append(y.data(), y.size()); //y
new_suffix.append(read.Suffix());
read.SetSuffix(new_suffix);
} else if (numDiscards == 1 && y.find('.') != re2::StringPiece::npos) {
string new_suffix;
new_suffix.append(re.GetMatch()[7].data(), re.GetMatch()[7].size()); // sep4
new_suffix.append(y.data(), y.size()); //y
new_suffix.append(read.Suffix());
read.SetSuffix(new_suffix);
}
}
m_tmp_spot.clear();
if (numDiscards == 1) {
assert(!prefix.empty());
if (sub_re1.Matches(prefix)) {
m_tmp_spot.append(sub_re1.GetMatch()[0].data(),
sub_re1.GetMatch()[0].size());
m_tmp_spot.append(sub_re1.GetMatch()[1].data(),
sub_re1.GetMatch()[1].size());
m_tmp_spot.append(sub_re1.GetMatch()[2].data(),
sub_re1.GetMatch()[2].size());
} else {
m_tmp_spot.append(prefix.data(), prefix.size());
}
s_add_sep(m_tmp_spot, re.GetMatch()[1]);
m_tmp_spot.append(lane.data(), lane.size());
s_add_sep(m_tmp_spot, re.GetMatch()[3]);
m_tmp_spot.append(tile.data(), tile.size());
s_add_sep(m_tmp_spot, re.GetMatch()[5]);
m_tmp_spot.append(x.data(), x.size());
} else if (numDiscards == 2) {
assert(!prefix.empty());
if (sub_re2.Matches(prefix)) {
m_tmp_spot.append(sub_re2.GetMatch()[0].data(),
sub_re2.GetMatch()[0].size());
m_tmp_spot.append(sub_re2.GetMatch()[1].data(),
sub_re2.GetMatch()[1].size());
m_tmp_spot.append(sub_re2.GetMatch()[2].data(),
sub_re2.GetMatch()[2].size());
m_tmp_spot.append(sub_re2.GetMatch()[3].data(),
sub_re2.GetMatch()[3].size());
m_tmp_spot.append(sub_re2.GetMatch()[4].data(),
sub_re2.GetMatch()[4].size());
} else if (sub_re3.Matches(prefix)) {
m_tmp_spot.append(sub_re3.GetMatch()[0].data(),
sub_re3.GetMatch()[0].size());
m_tmp_spot.append(sub_re3.GetMatch()[1].data(),
sub_re3.GetMatch()[1].size());
m_tmp_spot.append(sub_re3.GetMatch()[2].data(),
sub_re3.GetMatch()[2].size());
} else {
assert(false);
throw fastq_error(101, "Unexpected IlluminaOld Defline");
}
s_add_sep(m_tmp_spot, re.GetMatch()[1]);
m_tmp_spot.append(lane.data(), lane.size());
s_add_sep(m_tmp_spot, re.GetMatch()[3]);
m_tmp_spot.append(tile.data(), tile.size());
} else {
if (!prefix.empty()) {
m_tmp_spot.assign(prefix.data(), prefix.size());
s_add_sep(m_tmp_spot, re.GetMatch()[1]);
}
m_tmp_spot.append(lane.data(), lane.size()); //lane
s_add_sep(m_tmp_spot, re.GetMatch()[3]); // sep2
m_tmp_spot.append(tile.data(), tile.size()); //tile
s_add_sep(m_tmp_spot, re.GetMatch()[5]); // sep3
m_tmp_spot.append(x.data(), x.size()); //x
s_add_sep(m_tmp_spot, re.GetMatch()[7]); //sep 4
m_tmp_spot.append(y.data(), y.size()); //y
}
read.MoveSpot(std::move(m_tmp_spot));
if (!readNum.empty()) { // readNum
readNum.remove_prefix(1);
read.SetReadNum(readNum);
}
if (!re.GetMatch()[9].empty()) {
re.GetMatch()[9].remove_prefix(1); // spotGroup
read.SetSpotGroup(re.GetMatch()[9]);
}
}
int countExtraNumbersInIllumina(re2::StringPiece& prefix, re2::StringPiece& sep_str, re2::StringPiece& x, re2::StringPiece& y)
{
if (sep_str.empty())
return 0;
const char sep = (sep_str[0] == '-') ? ':' : sep_str[0];
// Determine how many numbers at the end of prefix
// separated by sep
sharq::split(prefix, m_tmp_strlist, sep);
int numCount = 0;
// Determine if prefix ends in one or two digits
// delineated with 'sep'
auto sz = m_tmp_strlist.size();
if (sz == 0)
return 0;
if (s_is_number(m_tmp_strlist[sz - 1]))
++numCount;
if (sz > 1 && s_is_number(m_tmp_strlist[sz - 2]))
++numCount;
// Determine how many numbers to discard (capping at 2 based on what we have seen in the data)
int discardCount = 0;
if (numCount) {
sharq::split(y, m_tmp_strlist, '.');
if (!m_tmp_strlist.empty() && atoi(m_tmp_strlist.front().data()) < 4) {
discardCount += 1;
if (numCount == 2) {
sharq::split(x, m_tmp_strlist, '.');
if (!m_tmp_strlist.empty() && atoi(m_tmp_strlist.front().data()) < 4)
discardCount += 1;
}
}
}
return discardCount;
}
private:
vector<string_view> m_tmp_strlist;
re2::StringPiece m_tmp_suffix;
};
class CDefLineMatcherIlluminaNew : public CDefLineMatcherIlluminaNewBase
/// illuminaNew matcher
{
public:
CDefLineMatcherIlluminaNew() :
CDefLineMatcherIlluminaNewBase(
"illuminaNew",
R"(^[@>+]([!-~]+?)([:_])(\d+)([:_])(\d+)([:_])(-?\d+\.?\d*)([:_])(-?\d+\.\d+|\d+)(\s+|[:_|-])([12345]|):([NY]):(\d+|O):?([!-~]*?)(\s+|$))")
{}
};
class CDefLineMatcherIlluminaNewNoPrefix : public CDefLineMatcherIlluminaNewBase
/// illuminaNewNoPrefix matcher
{
public:
CDefLineMatcherIlluminaNewNoPrefix() :
CDefLineMatcherIlluminaNewBase(
"illuminaNewNoPrefix",
R"(^[@>+]([!-~]*?)(:?)(\d+)([:_])(\d+)([:_])(\d+)([:_])(\d+)(\s+|_)([12345]|):([NY]):(\d+|O):?([!-~]*?)(\s+|$))")
{}
};
class CDefLineMatcherIlluminaNewWithSuffix : public CDefLineMatcherIlluminaNewBase
/// IlluminaNewWithSuffix (aka IlluminaNewWithJunk in fastq-load.py) matcher
{
public:
CDefLineMatcherIlluminaNewWithSuffix() :
CDefLineMatcherIlluminaNewBase(
"illuminaNewWithSuffix",
R"(^[@>+]([!-~]+)([:_])(\d+)([:_])(\d+)([:_])(-?\d+\.?\d*)([:_])(-?\d+\.\d+|\d+)([!-/:-~][!-~]*?\s+|[!-/:-~][!-~]*?[:_|-])([12345]|):([NY]):(\d+|O):?([!-~]*?)(\s+|$))"),
mSuffixPattern("(#[!-~]*?|)(/[12345]|\\[12345])?([!-~]*?)(#[!-~]*?|)(/[12345]|\\[12345])?([:_|]?)(\\s+|$)")
{
}
virtual void GetMatch(CFastqRead& read) override
{
CDefLineMatcherIlluminaNewBase::GetMatch(read);
const auto &m9 = re.GetMatch()[9];
if ( m9.size() > 2 ) {
if ( mSuffixPattern.Matches( m9 ) ) {
read.SetSuffix( mSuffixPattern.GetMatch()[2] );
}
}
}
private:
CRegExprMatcher mSuffixPattern;
};
// if (re2::RE2::PartialMatchN(re.GetMatch()[9], mSuffixPattern, &mSuffixArgs[0], (int)mSuffixArgs.size())) {
// read.SetSuffix(mSuffixMatch[2]);
// }
// }
// }
// re2::RE2 mSuffixPattern{"(#[!-~]*?|)(/[12345]|\\[12345])?([!-~]*?)(#[!-~]*?|)(/[12345]|\\[12345])?([:_|]?)(\\s+|$)"};
// vector<re2::RE2::Arg> mSuffixArgv;
// vector<re2::RE2::Arg*> mSuffixArgs;
// vector<re2::StringPiece> mSuffixMatch;
class CDefLineMatcherIlluminaNewWithPeriods : public CDefLineMatcherIlluminaNewBase
/// illuminaNewWithPeriods matcher
{
public:
CDefLineMatcherIlluminaNewWithPeriods() :
CDefLineMatcherIlluminaNewBase(
"illuminaNewWithPeriods",
"^[@>+]([!-~]+?)(\\.)(\\d+)(\\.)(\\d+)(\\.)(\\d+)(\\.)(\\d+)(\\s+|_)([12345]|)\\.([NY])\\.(\\d+|O)\\.?([!-~]*?)(\\s+|$)")
{}
};
class CDefLineMatcherIlluminaNewWithUnderscores : public CDefLineMatcherIlluminaNewBase
/// illuminaNewWithUnderscores matcher
{
public:
CDefLineMatcherIlluminaNewWithUnderscores() :
CDefLineMatcherIlluminaNewBase(
"illuminaNewWithUnderscores",
"^[@>+]([!-~]+?)(_)(\\d+)(_)(\\d+)(_)(\\d+)(_)(\\d+)(\\s+|_)([12345]|)_([NY])_(\\d+|O)_?([!-~]*?)(\\s+|$)")
{}
};
class CDefLineMatcherIlluminaOldColon : public CDefLineMatcherIlluminaOldBase
/// illuminaOldColon
{
public:
CDefLineMatcherIlluminaOldColon() :
CDefLineMatcherIlluminaOldBase(
"IlluminaOldColon",
R"(^[@>+]?([!-~]+?)(:)(\d+)(:)(\d+)(:)(-?\d+\.?\d*)([-:])(-?\d+\.\d+|-?\d+)_?[012]?(#[!-~]*?|)\s?(/[12345]|\\[12345])?(\s+|$))")
{}
};
class CDefLineMatcherIlluminaOldUnderscore : public CDefLineMatcherIlluminaOldBase
/// IlluminaOldUnderscore
{
public:
CDefLineMatcherIlluminaOldUnderscore() :
CDefLineMatcherIlluminaOldBase(
"IlluminaOldUnderscore",
R"(^[@>+]?([!-~]+?)(_)(\d+)(_)(\d+)(_)(-?\d+\.?\d*)(_)(-?\d+\.\d+|-?\d+)(#[!-~]*?|)\s?(/[12345]|\\[12345])?(\s+|$))")
{}
};
class CDefLineMatcherIlluminaOldNoPrefix : public CDefLineMatcherIlluminaOldBase
/// IlluminaOldUnderscore
{
public:
CDefLineMatcherIlluminaOldNoPrefix() :
CDefLineMatcherIlluminaOldBase(
"IlluminaOldNoPrefix",
R"(^[@>+]?([!-~]*?)(:?)(\d+)(:)(\d+)(:)(-?\d+\.?\d*)(:)(-?\d+\.\d+|-?\d+)(#[!-~]*?|)\s?(/[12345]|\\[12345])?(\s+|$))")
{}
};
class CDefLineMatcherIlluminaOldWithSuffix : public CDefLineMatcherIlluminaOldBase
/// IlluminaOldUnderscore
{
public:
CDefLineMatcherIlluminaOldWithSuffix() :
CDefLineMatcherIlluminaOldBase(
"IlluminaOldWithSuffix",
R"(^[@>+]?([!-~]+?)(:)(\d+)(:)(\d+)(:)(-?\d+\.?\d*)(:)(-?\d+\.\d+|-?\d+)(#[!-~]*?|)(/[12345][!-~]+)(\s+|$))")
{}
};
class CDefLineMatcherIlluminaOldWithSuffix2 : public CDefLineMatcherIlluminaOldBase
/// IlluminaOldUnderscore
{
public:
CDefLineMatcherIlluminaOldWithSuffix2() :
CDefLineMatcherIlluminaOldBase(
"IlluminaOldWithSuffix2",
R"(^[@>+]?([!-~]+?)(:)(\d+)(:)(\d+)(:)(-?\d+\.?\d*)(:)(-?\d+\.?\d*[!-~]+?)(#[!-~]*?|)\s?(/[12345]|\\[12345])?(\s+|$))")
{}
};
class CDefLineMatcherBgiOld : public CDefLineMatcher
/// BgiOld matcher
{
public:
CDefLineMatcherBgiOld() :
CDefLineMatcher(
"BgiOld",
R"(^[@>+](\S{1,3}\d{9}\S{0,3})(L\d)(C\d{3})(R\d{3})([_]?\d{1,8})(#[!-~]*?|)(/[1234]\S*|)(\s+|$))")
{
}
uint8_t GetPlatform() const override {
return 0;//SRA_PLATFORM_UNDEFINED
};
virtual void GetMatch(CFastqRead& read) override
{
// flowcell, lane, column, row, readNo, spotGroup, readNum, endSep
m_tmp_spot.clear();
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); //flowcell
m_tmp_spot.append(re.GetMatch()[1].data(), re.GetMatch()[1].size()); //lane
m_tmp_spot.append(re.GetMatch()[2].data(), re.GetMatch()[2].size()); //column
m_tmp_spot.append(re.GetMatch()[3].data(), re.GetMatch()[3].size()); //row
m_tmp_spot.append(re.GetMatch()[4].data(), re.GetMatch()[4].size()); // readNo
read.MoveSpot(std::move(m_tmp_spot));
if (!re.GetMatch()[6].empty() && absl::StartsWith(re.GetMatch()[6], "/"))
re.GetMatch()[6].remove_prefix(1);
read.SetReadNum(re.GetMatch()[6]);
if (!re.GetMatch()[5].empty())
re.GetMatch()[5].remove_prefix(1); // spotGroup
read.SetSpotGroup(re.GetMatch()[5]);
}
};
class CDefLineMatcherBgiNew : public CDefLineMatcher
/// BgiNew matcher
{
public:
CDefLineMatcherBgiNew() :
CDefLineMatcher(
"BgiNew",
R"(^[@>+](\S{1,3}\d{9}\S{0,3})(L\d)(C\d{3})(R\d{3})([_]?\d{1,8})(\S*)(\s+|[_|-])([12345]|):([NY]):(\d+|O):?([!-~]*?)(\s+|$))")
{}
uint8_t GetPlatform() const override {
return 0;//SRA_PLATFORM_UNDEFINED
};
virtual void GetMatch(CFastqRead& read) override
{
// 0 1 2 3 4 5 6 7 8 9 10 11
// flowcell, lane, column, row, readNo, suffix, sep1, readNum, filterRead, reserved, spotGroup, endSep
m_tmp_spot.clear();
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); //flowcell
m_tmp_spot.append(re.GetMatch()[1].data(), re.GetMatch()[1].size()); //lane
m_tmp_spot.append(re.GetMatch()[2].data(), re.GetMatch()[2].size()); //column
m_tmp_spot.append(re.GetMatch()[3].data(), re.GetMatch()[3].size()); //row
m_tmp_spot.append(re.GetMatch()[4].data(), re.GetMatch()[4].size()); // readNo
read.MoveSpot(std::move(m_tmp_spot));
read.SetSuffix(re.GetMatch()[5]);
read.SetReadNum(re.GetMatch()[7]);
read.SetSpotGroup(re.GetMatch()[10]);
read.SetReadFilter(re.GetMatch()[8] == "Y" ? 1 : 0);
}
};
// NANOPORE
class CDefLineMatcherNanoporeBase : public CDefLineMatcher
{
public:
CDefLineMatcherNanoporeBase( const string& defLineName, const string& pattern )
: CDefLineMatcher( defLineName, pattern ),
getPorePass( R"(pass[/\\])" ),
getPoreFail( R"(fail[/\\])" ),
getPoreBarcode2( R"((NB\d{2}|BC\d{2}|barcode\d{2})([/\\]))" )
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_OXFORD_NANOPORE;
};
void PostProcess( CFastqRead& read )
{ // Common Nanopore processing (done after parsing the defline)
if ( read.Channel().empty() )
{
read.SetChannel( "0" );
}
if ( read.NanoporeReadNo().empty() )
{
read.SetNanoporeReadNo( "0" );
}
// Set readNum to None if actually a file number or missing
if ( poreMid == "_file" ||
read.NanoporeReadNo().empty() )
{
read.SetNanoporeReadNo( "0" );
}
// Process poreFile if present
if ( ! poreFile.empty() )
{
// Check for 'pass' or 'fail'
if ( getPorePass.Matches( poreFile ) )
{
read.SetReadFilter( 0 );
}
else if ( getPoreFail.Matches( poreFile ) )
{
read.SetReadFilter( 1 );
}
// Check for barcode
if ( getPoreBarcode2.Matches( poreFile ) )
{
re2::StringPiece barcode = getPoreBarcode2.GetMatch()[0];
const string Barcode = "barcode";
if ( barcode.find( Barcode ) == 0 )
{ // self.spotGroup = re.sub(r'barcode(\d+)$',r'BC\1',self.spotGroup,1)
read.SetSpotGroup( string( "BC" )
+ string(barcode.data() + Barcode.size(),
barcode.size() - Barcode.size()) );
}
else
{
read.SetSpotGroup( barcode );
}
}
}
// # Split poreFile on '/' or '\' if present
// poreFileChunks = re.split(r'[/\\]',self.poreFile)
// if len ( poreFileChunks) > 1:
// self.poreFile = poreFileChunks.pop()
//TODO: ... and then self.poreFile does not seem to be used anywhere (ask Bob)
//TODO: where does appendPoreReadToName come from? (ask Bob)
// if self.poreRead and self.appendPoreReadToName:
// self.name += self.suffix + self.poreRead
// # Check for missing poreRead (from R-based poRe fastq dump) and normalize read type
// if ( poreRead.empty() )
// {
//TODO: where does filename come from? (ask Bob)
// if self.filename:
// if self.pore2Dpresent.search(self.filename):
// self.poreRead = "2D"
// elif self.poreTemplatePresent.search(self.filename):
// self.poreRead = "template"
// elif self.poreComplementPresent.search(self.filename):
// self.poreRead = "complement"
// else:
// self.poreRead = ""
// if self.poreRead and self.appendPoreReadToName:
// self.name += self.suffix + "_" + self.poreRead //AB: this might happen twice
// else:
// self.poreRead = ""
}
// elif ( self.poreRead == "_twodirections" or
// self.poreRead[1:] == "2D" or
// self.poreRead[0:3] == "_2d" ):
// self.poreRead = "2D"
// elif ( self.poreRead[0:9] == "_template" or
// self.poreRead == "-1D" or
// self.poreRead == ".1T" ):
// self.poreRead = "template"
// else:
// self.poreRead = "complement"
// if ( not self.deflineType and
// self.saveDeflineType ):
// self.deflineType = self.NANOPORE
// }
//TODO: ... and then self.poreRead does not seem to be used anywhere (ask Bob)
re2::StringPiece poreMid;
string poreFile;
re2::StringPiece poreRead;
CRegExprMatcher getPorePass;
CRegExprMatcher getPoreFail;
CRegExprMatcher getPoreBarcode2;
};
class CDefLineMatcherNanopore_Basic : public CDefLineMatcherNanoporeBase
{
public:
CDefLineMatcherNanopore_Basic( const string& defLineName, const string& pattern )
: CDefLineMatcherNanoporeBase( defLineName, pattern ), getPoreReadNo2( R"(read_?(\d+))" )
{
}
virtual void GetMatch(CFastqRead& read) override
{
//re.DumpMatch();
// 0 poreStart
// 1 self.channel
// 2 poreMid
// 3 self.readNo
// 4 poreEnd
// 5 self.poreRead
// 6 self.poreFile
// 7 endSep
m_tmp_spot.clear();
if ( !re.GetMatch()[3].empty() )
{ // self.name = poreStart + self.channel + poreMid + self.readNo + poreEnd
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); //poreStart
m_tmp_spot.append(re.GetMatch()[1].data(), re.GetMatch()[1].size()); //channel
m_tmp_spot.append(re.GetMatch()[2].data(), re.GetMatch()[2].size()); //poreMid
m_tmp_spot.append(re.GetMatch()[3].data(), re.GetMatch()[3].size()); //readNo
m_tmp_spot.append(re.GetMatch()[4].data(), re.GetMatch()[4].size()); //poreEnd
read.SetNanoporeReadNo( re.GetMatch()[3] );
}
else
{ // self.name = poreStart + self.channel + poreEnd
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); //poreStart
m_tmp_spot.append(re.GetMatch()[1].data(), re.GetMatch()[1].size()); //channel
m_tmp_spot.append(re.GetMatch()[4].data(), re.GetMatch()[4].size()); //poreEnd
if ( getPoreReadNo2.Matches(re.GetLastInput()) )
{
read.SetNanoporeReadNo( getPoreReadNo2.GetMatch()[0] );
}
}
read.MoveSpot(std::move(m_tmp_spot));
read.SetChannel( re.GetMatch()[1] );
poreMid = re.GetMatch()[2];
poreRead = re.GetMatch()[5];
poreFile.append(re.GetMatch()[6].data(), re.GetMatch()[6].size());
PostProcess( read );
}
CRegExprMatcher getPoreReadNo2;
};
class CDefLineMatcherNanopore1 : public CDefLineMatcherNanopore_Basic
{
public:
CDefLineMatcherNanopore1() : CDefLineMatcherNanopore_Basic(
"Nanopore1",
R"([@>+]+?(channel_)(\d+)(_read_)?(\d+)?([!-~]*?)(_twodirections|_2d|-2D|_template|-1D|_complement|-complement|\.1C|\.1T|\.2D)?(:[!-~ ]+?_ch\d+_file\d+_strand.fast5)?(\s+|$))"
) {}
};
class CDefLineMatcherNanopore2 : public CDefLineMatcherNanopore_Basic
{
public:
CDefLineMatcherNanopore2() : CDefLineMatcherNanopore_Basic(
"Nanopore2",
R"([@>+]([!-~]*?ch)(\d+)(_file)(\d+)([!-~]*?)(_twodirections|_2d|-2D|_template|-1D|_complement|-complement|\.1C|\.1T|\.2D)(:[!-~ ]+?_ch\d+_file\d+_strand.fast5)?(\s+|$))"
) {}
};
class CDefLineMatcherNanopore3 : public CDefLineMatcherNanoporeBase
{
public:
CDefLineMatcherNanopore3() :
CDefLineMatcherNanoporeBase(
"Nanopore3",
R"([@>+]([!-~]*?)[: ]?([!-~]+?Basecall)(_[12]D[_0]*?|_Alignment[_0]*?|_Barcoding[_0]*?|)(_twodirections|_2d|-2D|_template|-1D|_complement|-complement|\.1C|\.1T|\.2D|)[: ]([!-~]*?)[: ]?([!-~ ]+?_ch)_?(\d+)(_read|_file)_?(\d+)(_strand\d*.fast5|_strand\d*.*|)(\s+|$))"
)
{}
virtual void GetMatch(CFastqRead& read) override
{
//re.DumpMatch();
// 0 prefix
// 1 self.name
// 2 self.suffix
// 3 self.poreRead
// 4 discard
// 5 poreStart
// 6 self.channel
// 7 poreMid
// 8 self.readNo
// 9 poreEnd
// 10 endSep
read.SetSpot( re.GetMatch()[1] );
read.SetSuffix( re.GetMatch()[2] );
// For now, poreRead is expected to be "_template", other variants will be passed to the regular fastq-load.py
read.SetChannel( re.GetMatch()[6] );
read.SetNanoporeReadNo( re.GetMatch()[8] );
poreMid = re.GetMatch()[7];
//self.poreFile = poreStart + self.channel + poreMid + self.readNo + poreEnd
poreFile.append(re.GetMatch()[5].data(), re.GetMatch()[5].size()); //poreStart
poreFile.append(re.GetMatch()[6].data(), re.GetMatch()[6].size()); //channel
poreFile.append(re.GetMatch()[7].data(), re.GetMatch()[7].size()); //poreMid
poreFile.append(re.GetMatch()[8].data(), re.GetMatch()[8].size()); //readNo
poreFile.append(re.GetMatch()[9].data(), re.GetMatch()[9].size()); //poreEnd
poreRead = re.GetMatch()[3];
PostProcess( read );
}
};
class CDefLineMatcherNanopore3_1 : public CDefLineMatcherNanoporeBase
{
public:
CDefLineMatcherNanopore3_1() :
CDefLineMatcherNanoporeBase(
"Nanopore3_1",
R"([@>+]([!-~]+?)[: ]?([!-~]+?Basecall)(_[12]D[_0]*?|_Alignment[_0]*?|_Barcoding[_0]*?|)(_twodirections|_2d|-2D|_template|-1D|_complement|-complement|\.1C|\.1T|\.2D|)[: ]([!-~]*?)[: ]?([!-~ ]+?_read_)(\d+)(_ch_)(\d+)(_strand\d*.fast5|_strand\d*.*)(\s+|$))"
)
{}
virtual void GetMatch(CFastqRead& read) override
{
//re.DumpMatch();
// 0 prefix
// 1 self.name
// 2 self.suffix
// 3 self.poreRead
// 4 discard
// 5 poreStart
// 6 self.readNo
// 7 poreMid
// 8 self.channel
// 9 poreEnd
// 10 endSep
read.SetSpot( re.GetMatch()[1] );
read.SetSuffix( re.GetMatch()[2] );
// For now, poreRead is expected to be "_template", other variants will be passed to the regular fastq-load.py
read.SetNanoporeReadNo( re.GetMatch()[6] );
read.SetChannel( re.GetMatch()[8] );
poreMid = re.GetMatch()[7];
// poreFile = poreStart + self.readNo + poreMid + self.channel + poreEnd
poreFile.append(re.GetMatch()[5].data(), re.GetMatch()[5].size()); //poreStart
poreFile.append(re.GetMatch()[6].data(), re.GetMatch()[6].size()); //readNo
poreFile.append(re.GetMatch()[7].data(), re.GetMatch()[7].size()); //poreMid
poreFile.append(re.GetMatch()[8].data(), re.GetMatch()[8].size()); //channel
poreFile.append(re.GetMatch()[9].data(), re.GetMatch()[9].size()); //poreEnd
poreRead = re.GetMatch()[3];
PostProcess( read );
}
};
class CDefLineMatcherNanopore4 : public CDefLineMatcherNanoporeBase
{
public:
CDefLineMatcherNanopore4() :
CDefLineMatcherNanoporeBase(
"Nanopore4",
R"([@>+]([!-~]*?\S{8}-\S{4}-\S{4}-\S{4}-\S{12}\S*[_]?\d?)[\s+[!-~ ]*?|]$)"
),
getPoreReadNo( R"(read[=_]?(\d+))" ),
getPoreChannel( R"(ch[=_]?(\d+))" ),
getPoreBarcode( R"(barcode=(\S+))" )
{}
virtual void GetMatch(CFastqRead& read) override
{
// 0 self.name
read.SetSpot( re.GetMatch()[0] );
if ( getPoreReadNo.Matches(re.GetLastInput()) )
{
read.SetNanoporeReadNo( getPoreReadNo.GetMatch()[0] );
}
if ( getPoreChannel.Matches(re.GetLastInput()) )
{
read.SetChannel( getPoreChannel.GetMatch()[0] );
}
const string Unclassified = string("unclassified");
if ( getPoreBarcode.Matches(re.GetLastInput()) &&
getPoreBarcode.GetMatch()[0] != Unclassified )
{
read.SetSpotGroup( getPoreBarcode.GetMatch()[0] );
}
PostProcess( read );
}
CRegExprMatcher getPoreReadNo;
CRegExprMatcher getPoreChannel;
CRegExprMatcher getPoreBarcode;
};
class CDefLineMatcherNanopore5 : public CDefLineMatcherNanoporeBase
{
public:
CDefLineMatcherNanopore5() :
CDefLineMatcherNanoporeBase(
"Nanopore5",
R"([@>+]([!-~]*?[0-9a-fA-F]{8}-[0-9a-fA-F]{4}-[0-9a-fA-F]{4}-[0-9a-fA-F]{4}-[0-9a-fA-F]{12}_Basecall)(_[12]D[_0]*?|_Alignment[_0]*?|_Barcoding[_0]*?)(_twodirections|_2d|-2D|_template|-1D|_complement|-complement|\.1C|\.1T|\.2D)\S*?$)"
)
{}
virtual void GetMatch(CFastqRead& read) override
{
// 0 self.name
// 1 self.suffix
// 2 self.poreRead }
read.SetSpot( re.GetMatch()[0] );
read.SetSuffix( re.GetMatch()[1] );
// For now, poreRead is expected to be "_template", other variants will be passed to the regular fastq-load.py
PostProcess( read );
}
};
// LS454
class CDefLineMatcherLS454 : public CDefLineMatcher
/// LS454 matcher
{
public:
CDefLineMatcherLS454() :
CDefLineMatcher(
"LS454",
R"(^[@>+]([!-~]+_|)([A-Z0-9]{7})(\d{2})([A-Z0-9]{5})(/[12345])?(\s+|$))")
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_454;
};
virtual void GetMatch(CFastqRead& read) override
{
// prefix, dateAndHash454, region454, xy454, readNum, endSep
// 0 1 2 3 4 5
m_tmp_spot.clear();
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); //prefix
m_tmp_spot.append(re.GetMatch()[1].data(), re.GetMatch()[1].size()); //dateAndHash454
m_tmp_spot.append(re.GetMatch()[2].data(), re.GetMatch()[2].size()); //region454
m_tmp_spot.append(re.GetMatch()[3].data(), re.GetMatch()[3].size()); //xy454
read.MoveSpot(std::move(m_tmp_spot));
auto& readNo = re.GetMatch()[4];
if (!readNo.empty()) {
readNo.remove_prefix(1);
read.SetReadNum(readNo);
}
}
};
/*
self.ionTorrent = re.compile(r"[@>+]([A-Z0-9]{5})(:)(\d{1,5})(:)(\d{1,5})([^#/\s]*)(#[!-~]*?|)(/[12345]|\\[12345]|[LR])?(\s+|$)")
self.ionTorrent2 = re.compile(r"[@>+]([A-Z0-9]{5})(:)(\d{1,5})(:)(\d{1,5})([!-~]*)(\s+|[_|])([12345]|):([NY]):(\d+):?([!-~]*?)(\s+|$)")
*/
class CDefLineMatcherIonTorrent : public CDefLineMatcher
/// ION_TORRENT
{
public:
CDefLineMatcherIonTorrent() :
CDefLineMatcher(
"IonTorrent",
R"(^[@>+]([A-Z0-9]{5})(:)(\d{1,5})(:)(\d{1,5})([^#/\s]*)(#[!-~]*?|)(/[12345]|\\[12345]|[LR])?(\s+|$))")
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_ION_TORRENT;
};
virtual void GetMatch(CFastqRead& read) override
{
// runId, sep1, row, sep2, column, readNum, endSep
// runId, sep1, row, sep2, column, suffix, spotGroup, readNum, endSep
// 0 1 2 3 4 5 6 7 8
m_tmp_spot.clear();
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); //runId
m_tmp_spot.append(re.GetMatch()[1].data(), re.GetMatch()[1].size()); //sep1
m_tmp_spot.append(re.GetMatch()[2].data(), re.GetMatch()[2].size()); //row
m_tmp_spot.append(re.GetMatch()[3].data(), re.GetMatch()[3].size()); //sep2
m_tmp_spot.append(re.GetMatch()[4].data(), re.GetMatch()[4].size()); //column
read.MoveSpot(std::move(m_tmp_spot));
auto& suffix = re.GetMatch()[5];
auto& spotGroup = re.GetMatch()[6];
if (!spotGroup.empty()) {
spotGroup.remove_prefix(1);
read.SetSpotGroup(spotGroup);
}
auto& readNum = re.GetMatch()[7];
static const re2::StringPiece readNum1{"1"};
static const re2::StringPiece readNum2{"2"};
if (readNum.empty() && !suffix.empty() && (suffix[0] == 'L' || suffix[0] == 'R')) {
assert(suffix.size() == 1 && (suffix[0] == 'L' || suffix[0] == 'R'));
read.SetReadNum( suffix == "L" ? readNum1 : readNum2 );
} else {
read.SetSuffix(suffix);
if (absl::StartsWith(readNum, "/") || absl::StartsWith(readNum, "\\"))
readNum.remove_prefix(1);
else if (readNum == "L")
readNum = "1";
else if (readNum == "R")
readNum = "2";
read.SetReadNum(readNum);
}
}
};
class CDefLineMatcherIonTorrent2 : public CDefLineMatcher
/// ION_TORRENT
{
public:
CDefLineMatcherIonTorrent2() :
CDefLineMatcher(
"IonTorrent2",
R"(^[@>+]([A-Z0-9]{5})(:)(\d{1,5})(:)(\d{1,5})([!-~]*)(\s+|[_|])([12345]|):([NY]):(\d+|O):?([!-~]*?)(\s+|$))")
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_ION_TORRENT;
};
virtual void GetMatch(CFastqRead& read) override
{
// runId, sep1, row, sep2, column, suffix, sep3, readNum, filterRead, reserved, spotGroup, endSep
// 0 1 2 3 4 5 6 7 8 9 10 11
m_tmp_spot.clear();
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); //runId
m_tmp_spot.append(re.GetMatch()[1].data(), re.GetMatch()[1].size()); //sep1
m_tmp_spot.append(re.GetMatch()[2].data(), re.GetMatch()[2].size()); //row
m_tmp_spot.append(re.GetMatch()[3].data(), re.GetMatch()[3].size()); //sep2
m_tmp_spot.append(re.GetMatch()[4].data(), re.GetMatch()[4].size()); //column
read.MoveSpot(std::move(m_tmp_spot));
read.SetSuffix(re.GetMatch()[5]);
read.SetReadNum(re.GetMatch()[7]);
read.SetReadFilter(re.GetMatch()[8] == "Y" ? 1 : 0);
read.SetSpotGroup(re.GetMatch()[10]);
}
};
class CDefLineMatcherPacBio : public CDefLineMatcher
/// PacBio
{
public:
CDefLineMatcherPacBio() :
CDefLineMatcher(
"PacBio",
R"(^[@>+](m\d{5,6}_\d{6}_[!-~]+?_c\d{33}_s\d+_[pX]\d/\d+/?\d*_?\d*|m\d{6}_\d{6}_[!-~]+?_c\d{33}_s\d+_[pX]\d[|/]\d+[|/]ccs[!-~]*?)(\s+|$))")
{
}
CDefLineMatcherPacBio(const string& defLineName, const string& pattern) :
CDefLineMatcher(defLineName, pattern)
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_PACBIO_SMRT;
};
virtual void GetMatch(CFastqRead& read) override
{
// Name
// 0
m_tmp_spot.clear();
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); // name
read.MoveSpot(std::move(m_tmp_spot));
}
};
class CDefLineMatcherPacBio2 : public CDefLineMatcherPacBio
/// PacBio2
{
public:
CDefLineMatcherPacBio2() :
CDefLineMatcherPacBio(
"PacBio2",
R"(^[@>+]([!-~]*?m\d{5,6}\S{0,3}_\d{6}_\d{6}\S*[/_]\d+[!-~]*?)(\s+|$))")
{
}
};
class CDefLineMatcherPacBio3 : public CDefLineMatcherPacBio
/// PacBio3
{
public:
CDefLineMatcherPacBio3() :
CDefLineMatcherPacBio(
"PacBio3",
R"(^[@>+]([!-~]*?m\d{5,6}\S{0,3}_\d{6}_\d{6}\S*[/_]\d+/ccs[!-~]*?)(\s+|$))")
{
}
};
class CDefLineMatcherPacBio4 : public CDefLineMatcherPacBio
/// PacBio4
{
public:
CDefLineMatcherPacBio4() :
CDefLineMatcherPacBio(
"PacBio4",
R"(^[@>+]([!-~]*?m\d{5,6}\S{0,3}_\d{6}_\d{6}\S*[/_]\d+/\d+_\d+[!-~]*?)(\s+|$))")
{
}
};
//self.illuminaOldBcRnOnly = re.compile(r"^[@>+]([!-~]+?)(#[!-~]+?)(/[12345]|\\[12345])(\s+|$)")
class CDefLineIlluminaOldBcRn : public CDefLineMatcher
/// IlluminaOld BarCode and ReadNum only
{
public:
CDefLineIlluminaOldBcRn() :
CDefLineMatcher(
"illuminaOldBcRnOnly",
R"(^[@>+]([!-~]+?)(#[!-~]+?)(/[1234]|\\[1234])(\s+|$))")
{
}
CDefLineIlluminaOldBcRn(const string& defLineName, const string& pattern) :
CDefLineMatcher(defLineName, pattern)
{
}
uint8_t GetPlatform() const override {
return SRA_PLATFORM_UNDEFINED;
};
virtual void GetMatch(CFastqRead& read) override
{
// Name SpotGroup ReadNum endSep
// 0 1 2 3
m_tmp_spot.clear();
m_tmp_spot.append(re.GetMatch()[0].data(), re.GetMatch()[0].size()); // name
read.MoveSpot(std::move(m_tmp_spot));
auto& spot_group = re.GetMatch()[1];
if (absl::StartsWith(spot_group, "#")) {
spot_group.remove_prefix(1);
read.SetSpotGroup(spot_group);
auto& read_num = re.GetMatch()[2];
auto sz = read_num.size();
if ((sz > 0 && read_num[0] == '/') || (sz > 1 && read_num[0] == '\\')) {
read_num.remove_prefix(1);
read.SetReadNum(read_num);
}
} else {
spot_group.remove_prefix(1);
read.SetReadNum(spot_group);
}
}
};
//self.illuminaOldBcOnly = re.compile(r"^[@>+]([!-~]+?)(#[!-~]+)(\s+|$)(.?)")
class CDefLineIlluminaOldBcOnly : public CDefLineIlluminaOldBcRn
/// IlluminaOld BarCode only
{
public:
CDefLineIlluminaOldBcOnly() :
CDefLineIlluminaOldBcRn(
"illuminaOldBcOnly",
R"(^[@>+]([!-~]+?)(#[!-~]+)(\s+|$)(.?))")
{
}
};
//self.illuminaOldRnOnly = re.compile(r"^[@>+]([!-~]+?)(/[12345]|\\[12345])(\s+|$)(.?)")
class CDefLineIlluminaOldRnOnly : public CDefLineIlluminaOldBcRn
/// IlluminaOld ReadNum only
{
public:
CDefLineIlluminaOldRnOnly() :
CDefLineIlluminaOldBcRn(
"illuminaOldRnOnly",
R"(^[@>+]([!-~]+?)(/[1234]|\\[1234])(\s+|$)(.?))")
{
}
};
#endif
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