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#include "GSam.h"
#include <ctype.h>
/*
//for bam1_t (re)allocation functions:
// sam_realloc_bam_data(), realloc_bam_data(), possibly_expand_bam_data()
#include "sam_internal.h"
//for parsing functions hts_str2uint() etc.:
#include "textutils_internal.h"
#define _get_bmem(type_t, x, b, l) if (possibly_expand_bam_data((b), (l)) < 0) \
GError("Error: cannot allocate SAM data\n"); \
*(x) = (type_t*)((b)->data + (b)->l_data); (b)->l_data += (l)
*/
#define _parse_err(cond, msg) if (cond) GError("Error [SAM parsing]: %s\n",msg);
#define _parse_warn(cond, msg) if (cond) GMessage("Warning [SAM parsing]: %s\n",msg);
#define _parse_mem_err() GError("Error [SAM parsing]: memory allocation problem!\n");
#define _cigOp(c) ((c)&BAM_CIGAR_MASK)
#define _cigLen(c) ((c)>>BAM_CIGAR_SHIFT)
GSamRecord::GSamRecord(const char* qname, int32_t gseq_tid,
int pos, bool reverse, GDynArray<uint32_t>& cigar,
const char* qseq, const char* quals):iflags(0), exons(1),
juncsdel(1),clipL(0), clipR(0), mapped_len(0) {
uint16_t flag=0;
novel=true;
b_hdr=NULL;
b=bam_init1();
size_t l_qname=strlen(qname);
size_t l_seq=0;
if (qseq!=NULL) {
l_seq=strlen(qseq);
}
//-- passed pos is 1-based
if (pos<=0 || gseq_tid<0) {
flag |= BAM_FUNMAP;
pos=-1;
}
pos--;
if (reverse) flag|=BAM_FREVERSE;
bam_set1(b, l_qname, qname, flag,
gseq_tid, pos, 60, cigar.Count(), cigar(),
-1, 0, 0, l_seq, qseq, quals, 0);
}
/*
GSamRecord::GSamRecord(const char* qname, int32_t samflags, int32_t g_tid,
int pos, int map_qual, const char* cigar, int32_t mg_tid, int mate_pos,
int insert_size, const char* qseq, const char* quals,
GVec<char*>* aux_strings):iflags(0), exons(1) {
novel=true;
b_hdr=NULL;
b=bam_init1();
b->core.tid=g_tid;
b->core.pos = (pos<=0) ? -1 : pos-1; //BAM is 0-based
b->core.qual=map_qual;
int l_qname=strlen(qname);
//from sam_parse1() in sam.c:
_parse_warn(l_qname <= 1, "empty query name");
_parse_err(l_qname > 255, "query name too long");
// resize large enough for name + extranul
if (possibly_expand_bam_data(b, l_qname + 4) < 0)
_parse_mem_err();
memcpy(b->data + b->l_data, qname, l_qname);
b->l_data += l_qname;
b->core.l_extranul = (4 - (b->l_data & 3)) & 3;
memcpy(b->data + b->l_data, "\0\0\0\0", b->core.l_extranul);
b->l_data += b->core.l_extranul;
b->core.l_qname = l_qname + b->core.l_extranul;
set_cigar(cigar); //this will also set core.bin
if (qseq!=NULL) add_sequence(qseq, strlen(qseq));
if (quals!=NULL) add_quals(quals); //quals must be given as Phred33
set_flags(samflags);
set_mdata(mg_tid, (int32_t)(mate_pos-1), (int32_t)insert_size);
if (aux_strings!=NULL) {
for (int i=0;i<aux_strings->Count();i++) {
add_aux(aux_strings->Get(i));
}
}
}
*/
/*
void GSamRecord::set_cigar(const char* str) {
//requires b->core.pos and b->core.flag to have been set properly PRIOR to this call
// also expects the b record memory to not be allocated already (fresh record creation)
if (b==NULL) GError("Error: invalid call to ::set_cigar() (b is NULL)\n");
//SAM header ptr is in b_hdr
char *p = const_cast<char*>(str);
bam1_core_t *c = &b->core;
int overflow = 0;
hts_pos_t cigreflen;
if (*p == '*') {
_parse_warn(!(c->flag&BAM_FUNMAP),
"mapped query must have a CIGAR; treated as unmapped");
c->flag |= BAM_FUNMAP;
cigreflen = 1;
}
else {
char* q;
uint i=0;
uint32_t *cigar;
size_t n_cigar = 0;
for (q = p; *p!=0; ++p)
if (!isdigit(*p)) ++n_cigar;
_parse_err( n_cigar == 0, "no CIGAR operations");
_parse_err(n_cigar >= 2147483647, "too many CIGAR operations");
c->n_cigar = n_cigar;
_get_bmem(uint32_t, &cigar, b, c->n_cigar * sizeof(uint32_t));
for (i = 0; i < c->n_cigar; ++i) {
int op;
cigar[i] = hts_str2uint(q, &q, 28, &overflow)<<BAM_CIGAR_SHIFT;
op = bam_cigar_table[(unsigned char)*q++];
if (op<0) parse_error("unrecognized CIGAR operator");
cigar[i] |= op;
}
// can't use bam_endpos() directly as some fields not yet set up
cigreflen = (!(c->flag&BAM_FUNMAP))? bam_cigar2rlen(c->n_cigar, cigar) : 1;
}
_parse_err(HTS_POS_MAX - cigreflen <= c->pos,
"read ends beyond highest supported position");
c->bin = hts_reg2bin(c->pos, c->pos + cigreflen, 14, 5);
}
void GSamRecord::add_sequence(const char* qseq, int slen) {
// ---- see sam_parse1() in htslib/sam.c for details
//must be called AFTER set_cigar (cannot replace existing sequence for now)
if (qseq==NULL) return; //should we ever care about this?
if (slen<0) slen=strlen(qseq);
if (strcmp(qseq, "*")!=0) {
b->core.l_qseq=slen;
hts_pos_t ql = bam_cigar2qlen(b->core.n_cigar, bam_get_cigar(b));
_parse_err(b->core.n_cigar && ql != b->core.l_qseq,
"CIGAR and query sequence are of different length");
if (b->core.n_cigar && b->core.l_qseq != ql)
GError("Error: CIGAR and sequence length are inconsistent!(%s:%s)\n",
bam_get_qname(b), qseq);
int v = (b->core.l_qseq + 1) >> 1;
uint8_t *t;
_get_bmem(uint8_t, &t, b, v);
unsigned int lqs2 = b->core.l_qseq&~1, i;
for (i = 0; i < lqs2; i+=2)
t[i>>1] = (seq_nt16_table[(unsigned char)qseq[i]] << 4) | seq_nt16_table[(unsigned char)qseq[i+1]];
for (; i < (unsigned int)b->core.l_qseq; ++i)
t[i>>1] = seq_nt16_table[(unsigned char)qseq[i]] << ((~i&1)<<2);
}
else b->core.l_qseq = 0;
}
void GSamRecord::add_quals(const char* quals) {
//must be called immediately AFTER add_sequence()
uint8_t *t;
//this will just append newly allocated mem to b->data:
_get_bmem(uint8_t, &t, b, b->core.l_qseq);
if (quals==NULL || strcmp(quals, "*") == 0) {
memset(t, 0xff, b->core.l_qseq);
return;
}
int qlen=strlen(quals);
_parse_err(qlen!=b->core.l_qseq, "SEQ and QUAL are of different length!" );
//uint8_t* p=bam_get_qual(b);
for (int i=0;i < b->core.l_qseq; i++) t[i] = quals[i]-33;
}
*/
void GSamRecord::add_aux(const char* str) {
//requires: being called AFTER add_quals() for built-from-scratch records
//--check the "// aux" section in sam_parse1() htslib/sam.c
char tag[2];
uint8_t abuf[512];
int strl=strlen(str);
//int doff = b->core.l_qname + b->core.n_cigar*4 + (b->core.l_qseq+1)/2 + b->core.l_qseq + b->l_aux;
//int doff0=doff;
if (strl < 6 || str[2] != ':' || str[4] != ':')
parse_error("missing colon in auxiliary data");
tag[0] = str[0]; tag[1] = str[1];
uint8_t atype = str[3];
uint8_t* adata=abuf;
int alen=0;
if (atype == 'A' || atype == 'a' || atype == 'c' || atype == 'C') { // c and C for backward compatibility
atype='A';
alen=1;
adata=(uint8_t*)&str[5];
}
else if (atype == 'I' || atype == 'i') {
long long x=strtoll(str+5, NULL, 10); //(long long)atoll(str + 5);
//long x=(long)atol(str + 5);
if (x < 0) {
if (x >= -127) {
atype='c';
abuf[0] = (int8_t)x;
alen=1;
}
else if (x >= -32767) {
atype = 's';
*(int16_t*)abuf = (int16_t)x;
alen=2;
}
else {
atype='i';
*(int32_t*)abuf = (int32_t)x;
alen=4;
if (x < -2147483648ll)
GMessage("Parse warning: integer %lld is out of range.", x);
}
} else { //x >=0
if (x <= 255) {
atype = 'C';
abuf[0] = (uint8_t)x;
alen=1;
}
else if (x <= 65535) {
atype='S';
*(uint16_t*)abuf = (uint16_t)x;
alen=2;
}
else {
atype='I';
*(uint32_t*)abuf = (uint32_t)x;
alen=4;
if (x > 4294967295ll)
GMessage("Parse warning: integer %lld is out of range.", x);
}
}
} //integer type
else if (atype == 'f') {
*(float*)abuf = (float)atof(str + 5);
alen = sizeof(float);
}
else if (atype == 'd') { //?
*(float*)abuf = (float)atof(str + 9);
alen=8;
}
else if (atype == 'Z' || atype == 'H') {
if (atype == 'H') { // check whether the hex string is valid
if ((strl - 5) % 2 == 1) parse_error("length of the hex string not even");
for (int i = 0; i < strl - 5; ++i) {
int c = toupper(str[5 + i]);
if (!((c >= '0' && c <= '9') || (c >= 'A' && c <= 'F')))
parse_error("invalid hex character");
}
}
memcpy(abuf, str + 5, strl - 5);
abuf[strl-5] = 0;
alen=strl-4;
}
else if (atype == 'B') { //FIXME
// -- see sam_parse_B_vals() function in htslib/sam.c
//Integer or numeric array -- too messy to parse again here
GMessage("Warning: sorry, B tags not supported yet.\n");
}
else parse_error("unrecognized aux type");
//this->add_aux(tag, atype, alen, adata);
bam_aux_append(b, tag, atype, alen, adata);
}//add_aux()
int interpret_CIGAR(char cop, int cl, int aln_start) {
// returns the number of bases "aligned" (matches or mismatches) from the read
// gpos = current genomic position (will end up as right coordinate on the genome)
// rpos = read position (will end up as the length of the read)
// cop = CIGAR operation, cl = operation length
int mbases = 0; //count "aligned" bases (includes mismatches)
int rpos = 0;
int gpos = aln_start;
int num_mismatches=0; //NM tag value = edit distance
switch (cop) {
case BAM_CDIFF: // X
num_mismatches+=cl;
//fall-through
case BAM_CMATCH: // M
//have to actually check for mismatches: num_mismatches+=count_mismatches;
//fall-through
case BAM_CEQUAL: // =
//printf("[%d-%d]", gpos, gpos + cl - 1);
gpos+=cl;
rpos+=cl;
++mbases;
break;
case BAM_CPAD:
// printf("[%d-%d]", pos, pos + cl - 1); // Spans positions, No Coverage
gpos+=cl;
break;
case BAM_CHARD_CLIP:
// printf("[%d]", pos); // No coverage
// gpos is not advanced by this operation
break;
case BAM_CSOFT_CLIP: // S
//soft clipped bases, present in SEQ
rpos+=cl;
break;
case BAM_CINS: // I
// No Coverage
// adds cl bases "throughput" but not genomic position "coverage" (gap in genomic seq)
// should also add cl to the number of "mismatches" (unaligned read bases)
num_mismatches+=cl;
// How you handle this is application dependent
// gpos is not advanced by this operation
rpos+=cl;
break;
case BAM_CDEL: // D
//deletion in reference sequence relative to the read (gap in read sequence)
// printf("[%d-%d]", pos, pos + cl - 1);
// Spans positions
num_mismatches+=cl;
gpos += cl;
break;
case BAM_CREF_SKIP: // N
// intron
//special skip operation, not contributing to "edit distance",
// printf("[%d-%d]", pos, pos + cl - 1); // Spans positions, No Coverage
// so num_mismatches is not updated
gpos+=cl;
break;
default:
fprintf(stderr, "Unhandled cigar_op %d:%d\n", cop, cl);
//printf("?");
}
return mbases;
} // interpret_CIGAR(), just a reference of CIGAR operations interpretation
void GSamRecord::setupCoordinates() {
if (!b) return;
const bam1_core_t *c = &b->core;
if (c->flag & BAM_FUNMAP) return; /* skip unmapped reads */
uint32_t *cigar = bam_get_cigar(b);
int l=0;
mapped_len=0;
clipL=0;
clipR=0;
start=c->pos+1; //genomic start coordinate, 1-based (BAM core.pos is 0-based)
int exstart=c->pos;
GSeg exon;
bool intron=false;
bool ins=false;
uint del=0;
uint prevdel=0;
for (uint i = 0; i < c->n_cigar; ++i) {
unsigned char op = _cigOp(cigar[i]);
switch(op) {
case BAM_CEQUAL: // =
case BAM_CDIFF: // X
case BAM_CMATCH: // M
l+=_cigLen(cigar[i]);
if(intron) { // op comes after intron --> update juncdel
GSeg deljunc(prevdel,0);
juncsdel.Add(deljunc);
}
intron=false;
ins=false;
del=0;
break;
case BAM_CDEL:
del=_cigLen(cigar[i]);
l+=del;
if (intron) { // deletion after intron --> update juncsdel
GSeg deljunc(prevdel,del);
juncsdel.Add(deljunc);
}
ins=false;
break;
case BAM_CINS: // I
//rpos+=cl; //gpos not advanced
//take care of cases where there is an ins within an intron
ins=true;
break;
case BAM_CREF_SKIP: // N
// exon ends here
if(!ins || !intron) { // insertion in the middle of an intron --> adjust last exon
exon.end=c->pos+l;
exon.start=exstart+1;
exons.Add( exon );
mapped_len+=exon.len();
}
has_Introns=true;
l += _cigLen(cigar[i]);
exstart=c->pos+l;
prevdel=del;
intron=true;
del=0;
break;
case BAM_CSOFT_CLIP: // S
soft_Clipped=true;
if (l) clipR=_cigLen(cigar[i]);
else clipL=_cigLen(cigar[i]);
intron=false; ins=false;
del=0;
break;
case BAM_CHARD_CLIP:
hard_Clipped=true;
intron=false; ins=false;
del=0;
break;
case BAM_CPAD:
//gpos+=cl;
break;
default:
int cl=_cigLen(cigar[i]);
fprintf(stderr, "Unhandled CIGAR operation %d:%d\n", op, cl);
}
}
exon.start=exstart+1;
exon.end=c->pos+l;
exons.Add(exon);
mapped_len+=exon.len();
end=exon.end; //genomic end coordinate
}
uint8_t* GSamRecord::find_tag(const char tag[2]) {
return bam_aux_get(this->b, tag);
}
int GSamRecord::remove_tag(const char tag[2]) {
uint8_t* p=bam_aux_get(this->b, tag);
if (p!=NULL) return bam_aux_del(this->b, p);
return 0;
}
char GSamRecord::tag_char(const char tag[2]) { //retrieve tag data as single char
uint8_t* s=find_tag(tag);
if (s) return ( bam_aux2A(s) );
return 0;
}
char GSamRecord::tag_char1(const char tag[2]) { //just the first char from Z type tags
uint8_t* s=bam_aux_get(this->b, tag);
if (s==NULL) return 0;
int type;
type = *s++;
if (s == 0) return 0;
if (type == 'A' || type == 'Z') return *(char*)s;
else return 0;
}
int64_t GSamRecord::tag_int(const char tag[2], int nfval) { //get the numeric value of tag
uint8_t *s=find_tag(tag);
if (s) return ( bam_aux2i(s) );
return nfval;
}
double GSamRecord::tag_float(const char tag[2]) { //get the float value of tag
uint8_t *s=bam_aux_get(this->b, tag);;
if (s) return ( bam_aux2f(s) );
return 0;
}
char* GSamRecord::tag_str(const char tag[2]) { //return string value for a tag
uint8_t *s=find_tag(tag);
if (s) return ( bam_aux2Z(s) );
return NULL;
}
char GSamRecord::spliceStrand() { // '+', '-' from the XS tag, or 0 if no XS tag
char c=tag_char1("XS");
if (c==0) {
//try minimap2's "ts" tag
char m=tag_char1("ts");
if (m=='+' || m=='-') {
if ((this->b->core.flag & BAM_FREVERSE) != 0) c=((m=='+') ? '-' : '+');
else c=m;
}
}
return ((c=='+' || c=='-') ? c : '.');
}
char* GSamRecord::sequence() { //user must free this after use
char *s = (char*)bam_get_seq(b);
char* qseq=NULL;
GMALLOC(qseq, (b->core.l_qseq+1));
int i;
for (i=0;i<(b->core.l_qseq);i++) {
int8_t v = bam_seqi(s,i);
qseq[i] = seq_nt16_str[v];
}
qseq[i] = 0;
return qseq;
}
char* GSamRecord::qualities() {//user must free this after use
char *qual = (char*)bam_get_qual(b);
char* qv=NULL;
GMALLOC(qv, (b->core.l_qseq+1) );
int i;
for(i=0;i<(b->core.l_qseq);i++) {
qv[i]=qual[i]+33;
}
qv[i]=0;
return qv;
}
char* GSamRecord::cigar() { //returns text version of the CIGAR string; must be freed by user
kstring_t str = KS_INITIALIZE;
if (b->core.n_cigar == 0) kputc('*', &str);
else {
for (uint i = 0; i < b->core.n_cigar; ++i) {
kputw(bam_get_cigar(b)[i]>>BAM_CIGAR_SHIFT, &str);
kputc(BAM_CIGAR_STR[bam_get_cigar(b)[i]&BAM_CIGAR_MASK], &str);
}
}
return str.s;
}
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