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#!/usr/bin/env perl
use strict;
use warnings;
use Carp;
use Getopt::Long qw(:config no_ignore_case bundling);
use File::Basename;
$ENV{PATH} .= ":/usr/lib/trinityrnaseq/trinity-plugins/slclust/bin/";
my $help_flag;
my $min_per_id = 99;
my $max_per_gap = 1;
my $min_per_length = 99;
my $usage = <<__EOUSAGE__;
############################################################################################################################
#
#
# --target <string> transcript fasta file (headers must have accessions that look like so: transcript_id;gene_id
# --query <string> Trinity.fa
#
# --min_per_id <int> min percent identity (default: $min_per_id)
# --max_per_gap <int> max percent gaps (default: $max_per_gap)
# --min_per_length <float> min percent of reference transcript length to align and still consider FL (default: $min_per_length)
#
# --forward_orient strand-specific assemblies, only consider the forward strand.
#
# --include_tiers write the top tiers file
#
# --allow_non_unique_mappings a single query transcript counts towards all transcripts encapsulated
#
# --reuse no prompting to reuse any existing blat output files, just use them.
# --no_reuse no prompting, and do NOT reuse any existing files.
#
# --out_prefix prefix for output files generated.
#
#############################################################################################################################
__EOUSAGE__
;
my $target;
my $query;
my $forward_orient = 0;
my $include_tiers = 0;
my $reuse_flag = 0;
my $no_reuse_flag = 0;
my $allow_non_unique_mappings = 0;
my $out_prefix;
&GetOptions ( 'h' => \$help_flag,
'target=s' => \$target,
'query=s' => \$query,
'min_per_id=i' => \$min_per_id,
'forward_orient' => \$forward_orient,
'max_per_gap=i' => \$max_per_gap,
'include_tiers' => \$include_tiers,
'allow_non_unique_mappings' => \$allow_non_unique_mappings,
'min_per_length=f' => \$min_per_length,
'reuse' => \$reuse_flag,
'no_reuse' => \$no_reuse_flag,
'out_prefix=s' => \$out_prefix,
);
unless ($target && $query) {
die $usage;
}
if ($help_flag) {
die $usage;
}
my $util_dir = "/usr/lib/trinityrnaseq/util";
my $found_all_tools = 1;
my @required_tools = qw (blat slclust);
foreach my $tool (@required_tools) {
my $path = `which $tool`;
unless ($path =~ /\w/) {
print STDERR "Error, cannot locate required tool: $tool\n";
$found_all_tools = 0;
}
}
unless ($found_all_tools) {
die "Error, cannot proceed due to tools missing from PATH setting. ";
}
main: {
unless ($out_prefix) {
$out_prefix = basename($query);
}
my $cmd = "$util_dir/blat_full_length_mappings.pl --target $target --query $query "
. " --min_per_id $min_per_id --min_per_length $min_per_length --max_per_gap $max_per_gap ";
if ($forward_orient) {
$cmd .= " --forward_orient ";
}
if ($allow_non_unique_mappings) {
$cmd .= " --allow_non_unique_mappings ";
}
if ($reuse_flag) {
$cmd .= " --reuse ";
}
elsif ($no_reuse_flag) {
$cmd .= " --no_reuse ";
}
$cmd .= " --out_prefix $out_prefix ";
$cmd .= " > $out_prefix.pslx.maps";
&process_cmd($cmd); ## generates: $query.pslx, $query.pslx.FL_entries, $query.pslx.maps
if ($include_tiers) {
## generate top tiers
$cmd = "$util_dir/blat_top_tier_genes.pl $out_prefix.pslx > $out_prefix.pslx.tiers";
&process_cmd($cmd);
}
## generate summary statistics:
$cmd = "$util_dir/blat_map_filter_with_isoforms.pl $out_prefix.pslx.maps | tee $out_prefix.pslx.maps.summary";
&process_cmd($cmd);
exit(0);
}
####
sub process_cmd {
my ($cmd) = @_;
print "CMD: $cmd\n";
my $ret = system($cmd);
if ($ret) {
die "Error, cmd: $cmd died with ret $ret";
}
return;
}
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