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#!/usr/bin/python3
# encoding: utf-8
from __future__ import (absolute_import, division,
print_function, unicode_literals)
import os, sys, re
import logging
import argparse
import collections
import numpy
import time
import TGraph
logger = logging.getLogger(__name__)
class TNode:
"""
generic Trinity graph node object representing a node in the Trinity isoform reconstruction graph
Node's are objects within a gene and can be shared among transcript isoforms.
instance members include:
tgraph : (TGraph obj) graph for the Trinity gene, which will hold the nodes.
transcripts: list(str) names of the isoforms that contains this node.
loc_node_id : (int) identifier of the node
seq : (str) nucleotide sequence for this node in the transcript
len : (int) length of the node sequence
prev : (set) node objects connected as parental nodes in the graph
next : (set) node objects connected as descendant nodes in the graph
class members include:
merged_nodeset_counter : (int) tracking nodes that get merged under squeeze operations.
"""
node_cache = dict()
merged_nodeset_counter = 0
all_nodes_counter = 0
def __init__(self, tgraph, transcript_id, loc_node_id, node_seq):
"""
constructor, but don't use directly.... instead, use TGraph.get_node() factory function
"""
if len(node_seq) == 0:
raise RuntimeError("Error, TNode instantiation requires node sequence of length > 0")
self.tgraph = tgraph
self.transcripts = set()
self.add_transcripts(transcript_id)
self.loc_node_id = loc_node_id
self.seq = node_seq
self.len = len(node_seq)
TNode.all_nodes_counter += 1
self._id = TNode.all_nodes_counter
#logger.info("{}\t{}".format(loc_node_id, node_seq))
self.prev = set()
self.next = set()
self.stashed_prev = set() # for manipulation during topological sorting
self.stashed_next = set()
self.touched = 0
self.dead = False
self.topological_order = -1 # updated on topological sorting
#########################
## various Node ID values
#########################
def get_id(self):
# a private unique identifier for all nodes
return self._id
def get_loc_id(self):
return self.loc_node_id
def set_loc_id(self, loc_node_id):
self.loc_node_id = loc_node_id
def get_gene_id(self):
return self.tgraph.get_gene_id()
def get_gene_node_id(self):
gene_id = self.get_gene_id()
loc_id = self.get_loc_id()
node_id = gene_id + "::" + loc_id
return node_id
def get_touched_val(self):
return self.touched
def is_dead(self):
return(self.dead)
def is_ancestral(self, node, visited=None):
if visited is None:
visited = set() #init round
#logger.debug("is_ancestral search from {} of node {}".format(self, node))
if node == self:
#logger.debug("node is self")
return True
if node in self.prev:
#logger.debug("node in self.prev")
return True
else:
#logger.debug("continuing search")
visited.add(self)
#logger.debug("visited: {}".format(visited))
for prev_node in self.prev:
#logger.debug("cascading towards prev_node: {}".format(prev_node))
if prev_node in visited:
#logger.debug("prev_node in visited")
pass
else:
#logger.debug("prev_node not in visited")
found = prev_node.is_ancestral(node, visited)
if found:
return True
return False
def is_descendant(self, node, visited=None):
if visited == None:
visited = set() # init round
if node == self:
return True
if node in self.next:
return True
else:
visited.add(self)
for next_node in self.next:
if next_node not in visited:
found = next_node.is_descendant(node, visited)
if found:
return True
return False
## Other accessors
def get_graph(self):
return self.tgraph
def get_seq(self):
return self.seq
def set_seq(self, seq):
self.seq = seq
def get_topological_order(self):
return self.topological_order
def set_topological_order(self, topo_order):
self.topological_order = topo_order
def get_transcripts(self):
return self.transcripts
def add_transcripts(self, transcript_name_or_set):
if type(transcript_name_or_set) is set:
self.transcripts.update(transcript_name_or_set)
elif type(transcript_name_or_set) is str:
self.transcripts.add(transcript_name_or_set)
else:
raise RuntimeError("Error, parameter must be a string or a set ")
def get_prev_nodes(self):
return set(self.prev)
def get_next_nodes(self):
return set(self.next)
def add_next_node(self, next_node_obj):
self.next.add(next_node_obj)
def remove_next_node(self, remove_node_obj):
self.next.remove(remove_node_obj)
def stash_next_node(self, stash_node_obj):
self.remove_next_node(stash_node_obj)
self.stashed_next.add(stash_node_obj)
def add_prev_node(self, prev_node_obj):
self.prev.add(prev_node_obj)
def remove_prev_node(self, remove_node_obj):
self.prev.remove(remove_node_obj)
def stash_prev_node(self, stash_node_obj):
self.remove_prev_node(stash_node_obj)
self.stashed_prev.add(stash_node_obj)
def restore_stashed_nodes(self):
self.prev.update(self.stashed_prev)
self.stashed_prev = set()
self.next.update(self.stashed_next)
self.stashed_next = set()
def get_prev_node_loc_ids(self):
loc_ids = list()
for node in self.get_prev_nodes():
loc_ids.append(node.get_loc_id())
return loc_ids
def get_next_node_loc_ids(self):
loc_ids = list()
for node in self.get_next_nodes():
loc_ids.append(node.get_loc_id())
return loc_ids
def __repr__(self):
return(self.loc_node_id)
## Touching nodes
def touch(self):
self.touched += 1
def untouch(self):
self.touched -= 1
def clear_touch(self):
self.touched = 0
def toString(self):
txt = str("prev: " + str(self.get_prev_node_loc_ids()) +
", me: " + str(self.get_loc_id()) +
", next: " + str(self.get_next_node_loc_ids()) +
", transcripts: " + str(self.transcripts) +
", " + self.get_seq())
if self.topological_order >= 0:
txt += ", topo_order={}".format(self.topological_order)
if self.dead:
txt += " ** dead ** "
return txt
@classmethod
def merge_nodes(cls, node_list):
"""
Merges linear stretches of nodes into a single new node that has
concatenated sequences of the input nodes
"""
logger.debug("Merging nodes: {}".format(node_list))
merged_node_seq = ""
TNode.merged_nodeset_counter += 1
merged_loc_node_id = "M{}".format(TNode.merged_nodeset_counter)
# transcript list should be the intersection from nodes being merged (not the union)
# because repeat nodes could be part of the merge.
transcripts = node_list[0].get_transcripts()
for node_obj in node_list:
logger.debug("node being merge: {}".format(node_obj.toString()))
seq = node_obj.get_seq()
merged_node_seq += seq
transcripts = transcripts.intersection(node_obj.get_transcripts())
tgraph = node_list[0].get_graph()
merged_node = TNode(tgraph, transcripts, merged_loc_node_id, merged_node_seq)
return merged_node
def is_burr(self):
"""
returns true if node (x) is in this graphical context:
X X
\ or /
C-- A--? ?-- A--B
where X dangles.
So, X has only one parent or child and not otherwise connected in the graph.
"""
if self.get_prev_nodes() and self.get_next_nodes():
return False
if len(self.get_prev_nodes()) > 1 or len(self.get_next_nodes()) > 1:
return False
# illustration above on left side
if (len(self.get_next_nodes()) == 1
and
len(self.get_prev_nodes()) == 0
and
len(self.get_next_nodes().pop().get_prev_nodes()) > 1):
return True
# illustration above on right side
if (len(self.get_next_nodes()) == 0
and
len(self.get_prev_nodes()) == 1
and
len(self.get_prev_nodes().pop().get_next_nodes()) > 1):
return True
# more complex structure
return False
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