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#!/usr/bin/env perl
use strict;
use warnings;
my $usage = "\n\nusage: $0 chrysalis_dir/ component_no (WELDS|SCAFF|BOTH)\n\n";
my $chrysalis_dir = $ARGV[0] or die $usage;
my $component_no = $ARGV[1];
my $draw_type = $ARGV[2] or die $usage;
unless (defined $component_no) {
die $usage;
}
unless ($draw_type =~ /^(WELDS|SCAFF|BOTH)$/) {
die $usage;
}
main: {
my $graph_from_iworm_file = "$chrysalis_dir/GraphFromIwormFasta.out";
my %iworm_accs = &get_iworm_list_from_component($graph_from_iworm_file, $component_no);
## get the weld links:
my %welds = &parse_welds($graph_from_iworm_file, \%iworm_accs);
## get the scaffolding links:
my $scaffolding_file = "$chrysalis_dir/../iworm_scaffolds.txt";
my %scaffolds = &parse_scaffolded_pairs($scaffolding_file, \%iworm_accs);
## write dot file
print "digraph {\n";
my %iworm_to_id;
## write node labels
my $counter = 0;
foreach my $iworm_acc (keys %iworm_accs) {
$counter++;
my $iworm_id = $iworm_to_id{$iworm_acc} = $counter;
print "\t$iworm_id [label=\"$iworm_acc\"];\n";
}
if ($draw_type =~ /WELDS|BOTH/) {
## add weld links
foreach my $iworm_acc (keys %welds) {
my $iworm_id = $iworm_to_id{$iworm_acc};
foreach my $welded_iworm_acc (keys %{$welds{$iworm_acc}}) {
my $welded_id = $iworm_to_id{$welded_iworm_acc};
print "\t$iworm_id" . "->" . "$welded_id [color=\"blue\"];\n";
}
}
}
if ($draw_type =~ /SCAFF|BOTH/) {
## add scaffolding links
foreach my $iworm_acc (keys %scaffolds) {
my $iworm_id = $iworm_to_id{$iworm_acc};
unless (defined $iworm_id) { next; }
foreach my $scaffolded_iworm_acc (keys %{$scaffolds{$iworm_acc}}) {
my $scaffolded_id = $iworm_to_id{$scaffolded_iworm_acc};
unless (defined $scaffolded_id) { next; } # not all scaffolded entries were chosen for clustering if read support was insufficient
print "\t$iworm_id" . "->" . "$scaffolded_id [color=\"green\"];\n";
}
}
}
print "}\n";
exit(0);
}
####
sub parse_welds {
my ($graph_file, $iworm_accs_href) = @_;
my %welds;
open (my $fh, $graph_file) or die "Error, cannot oepn file $graph_file";
while (<$fh>) {
chomp;
if (/^\#Welding: >(a\d+;\d+)_\S+ to >(a\d+;\d+)_/) {
my $iworm_acc_A = $1;
my $iworm_acc_B = $2;
($iworm_acc_A, $iworm_acc_B) = sort ($iworm_acc_A, $iworm_acc_B);
if ($iworm_accs_href->{$iworm_acc_A} || $iworm_accs_href->{$iworm_acc_B}) { # really should only have situations where they're both included
$welds{$iworm_acc_A}->{$iworm_acc_B} = 1;
}
}
}
close $fh;
return(%welds);
}
####
sub get_iworm_list_from_component {
my ($graph_file, $component_no) = @_;
my %iworm_accs;
open (my $fh, $graph_file) or die "Error, cannot open file $graph_file";
while (<$fh>) {
if (/^>Component_$component_no /) {
/\[iworm>(a\d+;\d+)_/ or die "Error, cannot parse iworm acc from $_ ";
my $iworm_acc = $1;
$iworm_accs{$iworm_acc} = 1;
}
}
close $fh;
return(%iworm_accs);
}
####
sub parse_scaffolded_pairs {
my ($scaffolding_file, $iworm_accs_href) = @_;
my %scaffolded_pairs;
open (my $fh, $scaffolding_file) or die "Error, cannot open file $scaffolding_file";
while (<$fh>) {
chomp;
my @x = split(/\t/);
my ($iworm_acc_A, $iworm_acc_B) = ($x[0], $x[2]);
($iworm_acc_A, $iworm_acc_B) = sort ($iworm_acc_A, $iworm_acc_B);
if ($iworm_accs_href->{$iworm_acc_A} || $iworm_accs_href->{$iworm_acc_B}) {
$scaffolded_pairs{$iworm_acc_A}->{$iworm_acc_B} = 1;
}
}
close $fh;
return(%scaffolded_pairs);
}
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